CN105941334A - Method for incubation and rapid separation of Artemia nauplii - Google Patents

Abstract

The invention provides a method for incubation and rapid separation of Artemia nauplii. The method can realize high-level incubation rate and rapid separation of Artemia nauplii. According to a plurality of years of experiences of incubation and separation of Artemia in the process of fish breeding, directed at biological characteristics of Artemia, a novel scientific and reasonable method and technology with good operability for incubation and separation of Artemia are provided, so incubation rate and separation speed of Artemia nauplii are improved; and a stable and high-quality biological feed is provided for fry breeding of aquatic livestock.

Description

A method for hatching and rapidly separating Artemia nauplii

technical field

The invention relates to hatching and separation techniques of Artemia nauplii in the process of raising aquatic animal seedlings, in particular to a method for hatching and rapidly separating Artemia nauplii.

Background technique

Artemia (Brine Shrimp), also known as Saltwater Artemia, belongs to the phylum Arthropoda, with subphylum Braniopoda, Crustacea, Subclass Branchiopoda, No Armores, Saltline Artemia, and is an ideal biological bait for seawater fish and crustaceans , has received extensive attention. The hatching water temperature of Artemia is 25-30°C, and the salinity is 20-30. The separation of Artemia nauplii usually adopts the method of combining standing and light induction. When the eggs have hatched, the Artemia nauplii are collected from the hatching containers. First, take out the inflatable tube and the air stone from the incubator, and cover the top of the incubator with a black cloth to make the inside of the tank dark. After 10 to 15 minutes, siphon the mixture of nauplii and unhatched eggs from the bottom of the container into the sieve. , this process should try to avoid mixing empty shells. After the nauplii are collected, it is necessary to separate the mixed empty shells from the unhatched eggs, otherwise the empty shells will be swallowed by the fry and cause a large number of deaths. At present, the separation methods mainly include phototactic separation and specific gravity separation. Although these two methods can complete the separation of Artemia larvae, they have the disadvantages of long time and incomplete separation. It is very necessary to find out a new method with fast separation speed and high fractionation rate for the separation of Artemia larvae.

Contents of the invention

The purpose of the present invention is to provide a method for hatching and rapidly separating Artemia nauplii, which can realize a higher level of hatching rate and rapid separation of Artemia nauplii.

The technical solution adopted by the present invention to solve its technical problems is:

A method for hatching and rapidly separating Artemia nauplii, the method comprising the following steps:

①. Treatment of Artemia eggs before hatching

Use a glass fiber reinforced plastic tank with a conical bottom to hatch. Artemia eggs are soaked in fresh water for 1 to 3 hours before hatching to make the eggs fully absorb water. Before hatching, sterilize the eggs and soak them in 2-3% formalin for 10-3 hours. 15min;

②, Artemia eggs hatching

The hatching water temperature is maintained at 25-30°C, preferably 28°C; the seawater with a salinity of 20-30; the pH value is 7.5-8.5; an air stone is placed at the bottom of the hatching tank, and the air is continuously inflated during the hatching process to make the water tumbling to avoid A dead angle is formed at the bottom of the tank, and the density of high-quality eggs (hatch rate above 85%) is 3-4g/L. Artemia eggs are soaked in fresh water and fully absorbed, and lighted within 1 hour (generally, 2000lx artificial light is used to illuminate the top of the hatching tank) );

③, Artemia nauplius nutritional enhancement

Under the above conditions, after about 25 hours, the artemia nauplii begin to break their shells. At this time, the artemia nutritional enhancer is fully stirred and dissolved by the mixer, and then added to the hatching bucket for nutritional enhancement;

④ Rapid and efficient isolation of Artemia nauplii

As preferably, the process of step ④ is:

First, prepare a glass fiber reinforced plastic tank with a conical bottom and a 60-mesh silk bag with a length of 80 cm and a diameter of 30 cm. There is an outlet valve at the bottom of the tank and an inlet valve on the top, and 2/3 of clean seawater is added to the tank;

Secondly, after 12 hours of adding the nutrient enhancer, the incubator stopped aerating, covered the top of the incubator with a black cloth, and let it stand for 10 minutes; slowly opened the bottom of the incubator, drained the first unhatched eggs, Put a 120-mesh silk screen bag on the water outlet to collect the nauplii; when the liquid level in the container drops to the bottom of the cone, remove the screen bag and drain the egg shells; transfer the nauplii in the screen bag into a bucket filled with clean sea water;

Again, pour an appropriate amount of shelled Artemia nauplii in the bucket into the sieve bag, hold the mouth of the sieve bag tightly with your right hand, shake it vigorously in the prepared glass fiber reinforced plastic tank for several times, open the water inlet valve, and use The water flow concentrates to wash the nauplii and egg shells in the net bag, so that they are concentrated at the bottom of the net bag, and continue to shake several times in the glass fiber reinforced plastic tank. After 2-3 repetitions, the nauplii in the net bag will Throw it into the glass fiber reinforced plastic water tank, the egg shell stays in the mesh bag, drain the egg shell in the mesh bag, add the Artemia nauplii to be separated, continue to separate by this method, and complete the separation of all Artemia nauplii Finally, open the water outlet valve of the glass fiber reinforced plastic water tank, collect the artemia nauplii separated in the glass fiber reinforced plastic water tank with a 120-mesh sieve tulle bag, and put it into the seedling pond for feeding after being fully washed.

As a preference, the formula of the nutrition enhancer is: DL-methionine 1.2g/L, oleic acid 0.5 g/L, corn gluten powder 3 g/L, soybean protein powder 3 g/L, casein phosphopeptide 0.3 g/L, α-linolenic acid 0.2 g/L.

Preferably, in step ②, DO is maintained at a level of 2 mg/L to obtain the best hatching effect. The amount of inflation should be properly controlled so that the eggs can be evenly distributed and mechanical damage can be avoided.

Artemia is an indispensable biological bait in the breeding process of seawater fish and crustaceans, and its pre-hatching treatment, hatching, separation and strengthening all directly affect the hatching rate and survival rate of Artemia. The beneficial effect of the present invention is: the present invention proposes a scientific, reasonable and operable new method and technology for Artemia hatching and separation based on years of experience in hatching and separating Artemia in the process of fish breeding, aiming at the biological characteristics of Artemia , to improve the hatching rate and separation speed of Artemia larvae, and provide stable and high-quality biological bait for the breeding of aquatic animal seedlings.

detailed description

The technical solution of the present invention will be further specifically described below through specific examples. It should be understood that the implementation of the present invention is not limited to the following examples, and any modifications and/or changes made to the present invention will fall within the protection scope of the present invention.

In the present invention, unless otherwise specified, all parts and percentages are in weight units, and the equipment and raw materials used can be purchased from the market or commonly used in the field. The methods in the following examples, unless otherwise specified, are conventional methods in the art.

Example:

1. Treatment of Artemia eggs before hatching

Use a glass fiber reinforced plastic tank with a conical bottom to incubate, and the incubator should be sterilized before use. Artemia eggs are soaked in fresh water for 1 to 3 hours before hatching, so that the eggs can fully absorb water, and the eggs are disinfected before hatching, soaked in 2-3% formalin for 10-15 minutes.

2. Artemia eggs hatch

The hatching water temperature is maintained at 25-30°C; seawater with a salinity of 20-30; the pH value is preferably 7.5-8.5. Place enough air stones at the bottom of the hatching tank. During the hatching process, it needs to be continuously inflated to make the water roll and avoid forming a dead angle at the bottom of the tank. The best hatching effect can be obtained by maintaining DO at the level of 2mg/L. The amount of inflation should be properly controlled so that the eggs can be evenly distributed and mechanical damage can be avoided. The density of high-quality eggs (hatch rate above 85%) is generally 3g/L. Artemia ovum is soaked in fresh water and the light in 1h after fully absorbing water is important to improving hatching rate, generally adopts 2000lx artificial light to illuminate above the hatching tank.

3. Nutritional enhancement of Artemia nauplii

Under the above conditions, after about 25 hours, the Artemia nauplii began to break their shells. At this time, the Artemia nutritional enhancer was fully stirred and dissolved by the mixer, and then added to the hatching bucket for nutritional enhancement.

The formula of nutritional enhancer is: DL-methionine 1.2g/L, oleic acid 0.5 g/L, corn gluten powder 3 g/L, soybean protein powder 3 g/L, casein phosphopeptide 0.3 g/L, α-flax Acid 0.2 g/L.

4. Rapid and efficient isolation of Artemia nauplii

First, prepare a glass fiber reinforced plastic tank with a conical bottom and a 60-mesh silk bag with a length of 80 cm and a diameter of 30 cm. There is an outlet valve at the bottom of the tank and an inlet valve on the top, and 2/3 of clean seawater is added to the tank.

Secondly, 12 hours after adding the nutrient fortifier, the incubator stopped aerating, covered the top of the incubator with a black cloth, and left it for 10 minutes. Slowly open the hatch at the bottom of the hatching tank, drain the unhatched eggs that flow out first, put a 120-mesh sieve tulle bag on the water outlet, and collect the nauplii. When the liquid level in the container drops to the bottom of the cone, the sieve tulle bag is removed and the egg shells are drained. Transfer the nauplii in the silk bag to a bucket with clean seawater.

Again, pour an appropriate amount of shelled Artemia nauplii in the bucket into the sieve bag, hold the mouth of the sieve bag tightly with your right hand, shake it vigorously in the prepared glass fiber reinforced plastic tank for several times, open the water inlet valve, and use The water flow concentrates to wash the nauplii and egg shells in the net bag, so that they are concentrated at the bottom of the net bag, and continue to shake several times in the glass fiber reinforced plastic tank. After 2-3 repetitions, the nauplii in the net bag will Throw it into the glass fiber reinforced plastic water tank, the egg shell stays in the mesh bag, drain the egg shell in the mesh bag, add the Artemia nauplii to be separated, continue to separate by this method, and complete the separation of all Artemia nauplii Finally, open the water outlet valve of the glass fiber reinforced plastic water tank, collect the artemia nauplii separated in the glass fiber reinforced plastic water tank with a 120-mesh sieve tulle bag, and put it into the seedling pond for feeding after being fully washed.

The hatching rate of Artemia was 96.8%.

The embodiment described above is only a preferred solution of the present invention, and does not limit the present invention in any form. There are other variations and modifications on the premise of not exceeding the technical solution described in the claims.

Claims (4)

1. A method for hatching and rapidly separating Artemia nauplii is characterized in that the method comprises the steps: ①. Treatment of Artemia eggs before hatching Use a glass fiber reinforced plastic tank with a conical bottom to hatch. Artemia eggs are soaked in fresh water for 1 to 3 hours before hatching to make the eggs fully absorb water. Before hatching, sterilize the eggs and soak them in 2-3% formalin for 10-3 hours. 15min; ②, Artemia eggs hatching The hatching water temperature is maintained at 25-30°C; the seawater with a salinity of 20-30; the pH value is 7.5-8.5; an air stone is placed at the bottom of the hatching tank, and air is continuously inflated during the hatching process to make the water tumbling and avoid forming a dead angle at the bottom of the tank , the density of high-quality eggs is 3-4g/L, Artemia eggs are soaked in fresh water and fully absorbed, and then lighted within 1 hour; ③, Artemia nauplius nutritional enhancement Under the above conditions, after about 25 hours, the artemia nauplii begin to break their shells. At this time, the artemia nutritional enhancer is fully stirred and dissolved by the mixer, and then added to the hatching bucket for nutritional enhancement; ④ Rapid and efficient isolation of Artemia nauplii. 2. the hatching of artemia nauplii according to claim 1 and the method for fast separation, it is characterized in that the process of step 4. is: First, prepare a glass fiber reinforced plastic tank with a conical bottom and a 60-mesh silk bag with a length of 80 cm and a diameter of 30 cm. There is an outlet valve at the bottom of the tank and an inlet valve on the top, and 2/3 of clean seawater is added to the tank; Secondly, after 12 hours of adding the nutrient enhancer, the incubator stopped aerating, covered the top of the incubator with a black cloth, and let it stand for 10 minutes; slowly opened the bottom of the incubator, drained the first unhatched eggs, Put a 120-mesh silk screen bag on the water outlet to collect the nauplii; when the liquid level in the container drops to the bottom of the cone, remove the screen bag and drain the egg shells; transfer the nauplii in the screen bag into a bucket filled with clean sea water; Again, pour an appropriate amount of shelled Artemia nauplii in the bucket into the sieve bag, hold the mouth of the sieve bag tightly with your right hand, shake it vigorously in the prepared glass fiber reinforced plastic tank for several times, open the water inlet valve, and use The water flow concentrates to wash the nauplii and egg shells in the net bag, so that they are concentrated at the bottom of the net bag, and continue to shake several times in the glass fiber reinforced plastic tank. After 2-3 repetitions, the nauplii in the net bag will Throw it into the glass fiber reinforced plastic water tank, the egg shell stays in the mesh bag, drain the egg shell in the mesh bag, add the Artemia nauplii to be separated, continue to separate by this method, and complete the separation of all Artemia nauplii Finally, open the water outlet valve of the glass fiber reinforced plastic water tank, collect the artemia nauplii separated in the glass fiber reinforced plastic water tank with a 120-mesh sieve tulle bag, and put it into the seedling pond for feeding after being fully washed. 3. The method for hatching and rapidly separating Artemia nauplii according to claim 1, characterized in that: the formula of the nutrition enhancer is: DL-methionine 1.2g/L, oleic acid 0.5 g/L, corn gluten powder 3 g/L, soybean protein powder 3 g/L, casein phosphopeptide 0.3 g/L, α-linolenic acid 0.2 g/L. 4. The method for hatching and rapidly separating Artemia nauplii according to claim 1, characterized in that: in step ②, DO is maintained at a level of 2 mg/L.