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CN109599150A - A kind of peanut specific test approximation method for screening varieties based on phenotype distance - Google Patents

A kind of peanut specific test approximation method for screening varieties based on phenotype distance Download PDF

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Publication number
CN109599150A
CN109599150A CN201811462637.7A CN201811462637A CN109599150A CN 109599150 A CN109599150 A CN 109599150A CN 201811462637 A CN201811462637 A CN 201811462637A CN 109599150 A CN109599150 A CN 109599150A
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phenotypic
varieties
variety
peanut
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Inventor
张晗
李汝玉
段丽丽
王雪梅
王玮
孙加梅
郑永胜
王穆穆
王晖
李华
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Institute of Biotechnology of Fujian Academy of Agricultural Science
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Institute of Biotechnology of Fujian Academy of Agricultural Science
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Abstract

本发明公开了一种基于表型距离的花生特异性测试近似品种筛选方法。该方法根据各个表型性状的表达状态观测值受环境条件影响的程度和人员观测偏差的大小,对各个花生表型性状的所有表达状态的代码差值设置不同的权重(如表1),计算品种间有差异性状权重之和作为品种间的表型距离。通过对大量真实品种间表型距离和差异明显程度的比较分析,确定一个安全的表型距离阈值,低于该表型距离阈值的品种作为近似品种。采用这种方法,较好排除了人员观测偏差、互作和数据录入错误和对近似品种筛选的影响,提高了近似品种筛选的严谨性。采用该方法,便于田间试验时将最近似的品种与申请品种相邻种植,提高了特异性测试的针对性。

The invention discloses a method for screening similar varieties of peanuts based on phenotypic distance specificity test. This method sets different weights for the code differences of all expression states of each peanut phenotypic trait according to the degree to which the observed value of the expression state of each phenotypic trait is affected by environmental conditions and the magnitude of the deviation of personnel observations (as shown in Table 1), and calculates The sum of the weights of different traits among varieties was used as the phenotypic distance between varieties. Through the comparative analysis of the phenotypic distance and the obvious degree of difference between a large number of real varieties, a safe phenotypic distance threshold was determined, and the varieties below the phenotype distance threshold were regarded as similar varieties. By adopting this method, personnel observation bias, interaction and data entry errors and the influence on the screening of similar varieties are eliminated, and the rigor of screening of similar varieties is improved. By adopting this method, it is convenient to plant the most similar variety adjacent to the applied variety during the field test, which improves the pertinence of the specific test.

Description

A kind of peanut specific test approximation method for screening varieties based on phenotype distance
Technical field
The present invention relates to a kind of peanut specific test approximation method for screening varieties based on phenotype distance, it is new to belong to plant Kind test (or DUS test) technical field.
Background technique
Specificity, consistency and stability are the basic demands to plant variety, are that new variety of plant obtains kind power guarantor The precondition of shield, staple crops kind variety certification and the registration of non-principal variety of crops.Specificity refers to application kind All before the applying date known belong to or kind of the same race should be clearly distinguishable from.Have specificity for one kind of identification, needs Prove that the kind is different from other any kinds of known similar crop on phenotypic character.Kind known to chief crop is often It is ten hundreds of, by the kind (" application kind ") of each application DUS test compared with these known kinds carry out field planting, It is infeasible in practice.For this reason, it may be necessary to which those, which are not needed plantation, relatively can determine and apply by some Filtering systems The visibly different kind of kind phenotypic character excludes, and only planting those and not passing through field comparative test then not can determine that and application product Whether kind has the kind (i.e. approximate kind) of obvious phenotypic difference.It is tested by field planting, compares application kind and approximate product Kind whether there is notable difference, show whether application kind has the conclusion of specificity accordingly.Therefore, the selection of approximate kind is The key link of specific test.
Although kind is large number of known to chief crop, for most of known kinds, the especially product of breeding morning Kind, because the reasons such as yielding ability have been unable to meet current production needs, can not considered in approximate screening varieties.Approximate kind Screening generally have by comparing application kind and still the known kind (such as the kind being bred as nearly ten years) of application potential Trait expression state carries out.If can determine a known kind and apply kind expression status difference be big enough in Notable difference is inherently shown when field planting between the two, then can be excluded the known kind, remaining kind is as close Like kind.For quantitative character and false qualitative character (expression status in the consecutive variations region of especially false qualitative character), Following characteristics can be presented because of the influence of environmental condition and human factor in expression status observation: (1) same by environmental influence The same character of a kind, when different year and different regions are planted, expression status observation (expression status code) meeting Certain fluctuation occurs;(2) different trait expressions degree affected by environment is different, show as expression status degree of fluctuation because Character and it is different.(3) different testers are easy to produce deviation to the observed result of trait expression state.The size of deviation, with property Shape and personnel have relationship.How to determine approximate screening varieties index, is the difficult point of approximate screening varieties.A kind of way is basis The expression way of character, trait expression are influenced degree of fluctuation by environment degree and are influenced size by human error, by applying for product Kind screens approximate kind: for false qualitative character and quantitative character, using therewith compared with each expression status of known kind Differ the expression status range of 1-3 code.It is very high for the accuracy requirement of data using above-mentioned way.In practice, exist Individual character expression status data it is excessive or due to data inputting due to being influenced to deviate normal value by personnel's observed deviation Mistake, or due to the interaction (under rare occasion) of character and environmental condition, will lead to should be as the known product of approximate kind Kind fails screening and comes in, and causes the omission of approximate kind, directly affects the accuracy of specific test.In addition, to each kind Screening conditions be configured, process is cumbersome, and screening efficiency is lower.
Applicant develops the approximate method for screening varieties in a kind of peanut specific test based on phenotypic character before (application number of invention patent 201610061376.2).Approximate method for screening varieties of this method based on single phenotypic character, is root Degree of fluctuation is influenced by environment degree according to the expression way of character, trait expression and is influenced size by human error, for application The false qualitative character of each of kind and quantitative character, it is known that kind uses the expression status range for differing 1-3 code therewith, Through application kind compared with each expression status of known kind, approximate kind is screened.Applicant has found in follow-up study, This method has still had following deficiency, needs further to improve: (1) poor fault tolerance, very high to the accuracy requirement of data.It is real In trampling, there is the data of individual character expression status due to by personnel's observed deviation influenced deviate normal value it is excessive or by In data inputting mistake, or due to the interaction (under rare occasion) of character and environmental condition, will lead to should be as approximate product The known kind of kind fails screening and comes in, and causes the omission of approximate kind.(2) differential screening of this method based on single character is close Like kind, interracial difference cannot be added up, be selected into less approximate kind;(3) selected approximate kind cannot be shown With the degree of approximation of application kind, it is not easy to design field specific test test.
Summary of the invention
Aiming at the problem that the above-mentioned approximate method for screening varieties based on single character is easy to cause omission approximation kind, this hair It is bright to provide a kind of peanut specific test approximation method for screening varieties based on phenotype distance.This method is directed to kind dissimilarity Shape expression status observation is observed this different feature of influence degree by environment and personnel, between application kind and known kind The expression status code difference of selected phenotypic character gives certain weight, by application kind and known kind in one group of character Weight be added, obtain interracial phenotype distance.The size of weight depends on the character, expression status observation by environment and The size of personnel observation influence degree and code difference.Trait expression is more stable, observed deviation is smaller, and weight is bigger;Code is poor Value is bigger, and weight is bigger.By, there are the statistics of a large amount of test article inter-species phenotype distances of notable difference, finding out one to character It is a approximation screening varieties safe distance, guarantee be higher than the safe distance kind there are apparent differences.Screening approximate product When kind, using the safe distance as the threshold value of approximate screening varieties: the known kind higher than the safe distance is deposited with approximate kind In apparent difference, do not need to plant;Field is carried out as approximate kind and application kind less than the known kind of the safe distance Between adjacent plantation and Evaluation on specificity.In this way, personnel's observed deviation, interaction and data inputting mistake are preferably eliminated With the influence of pairing approximation screening varieties, the preciseness of approximate screening varieties is improved.
The technical scheme is that a kind of peanut specific test approximation method for screening varieties based on phenotype distance, It is characterized in that
(1) weight of each peanut phenotypic character code difference is set
The phenotypic character number of the current still peanut varieties (such as the kind being bred as nearly ten years) with application potential of acquisition According to (the high character of Variety identification power in DUS Testing Guideline, two growth cycles of each kind general test), to the table of acquisition Type trait data is arranged, and peanut varieties phenotype trait data library is established;It is observed according to the expression status of each phenotypic character Value is by the degree of environmental influence and the size of personnel's observed deviation, to all expression status of each peanut phenotypic character (such as code is 1-9 to code difference, and difference 0,1,2 ... 8) is arranged different weights, obtains data as shown in Table 1, is inputted Peanut varieties phenotype trait data library;
For qualitative character, when code difference is more than or equal to 1, weight 2-6, for quantitative character and false qualitative character, It is poor to different codes according to trait expression State Viewpoint measured value by the degree of environmental influence and the size of personnel's observed deviation Value, gives different weights.The weight of peanut difference DUS test character expression status difference is as follows:
The weight of 1 peanut difference DUS test character expression status difference of table
Note: 1.QN: quantitative character, PQ: false qualitative character;QL: qualitative character
2. "-" indicates do not have (weight of corresponding respective code difference)
(2) setting of phenotype distance threshold
To the phenotypic character expression status code difference of pairs of peanut varieties, weight is assigned by the method for step (1), is owned Weight addition on phenotypic character obtains two interracial phenotype distances;By the obvious journey of a large amount of phenotype distances and Differences Degree is compared, and obtains relationship between the two, determines a phenotype distance threshold on this basis: when phenotype distance between kind When greater than the threshold value, difference compares it is obvious that not needing field planting between kind;And by phenotype distance threshold input database System.
The present invention after years of research and found that, suitable phenotype distance threshold be 2, i.e., when between kind phenotype distance >=2 when, product Difference is apparent between kind;It is determined as approximate kind when two interracial phenotype distance < 2.
(3) approximate screening varieties
The approximate screening varieties software based on phenotype distance is developed in peanut varieties phenotype trait data library based on foundation. The phenotype trait data of application kind to be measured is inputted into peanut varieties phenotype trait data library, which can be according to step Suddenly the method for (2) calculates the phenotype distance of application kind to be measured and each known kind, and is compared with phenotype distance threshold Compared with phenotype distance is less than approximate kind of the known kind of threshold value as the application kind to be measured.
Method of the invention is different with influence degree of the personnel to various trait expression status observation according to environmental condition This feature gives a weight, the sum of variant character weight is used as kind between calculating kind to the difference of code kind Between phenotype distance.By the comparative analysis between phenotype distance and difference obvious degree a large amount of true kinds, a peace is determined Full phenotype distance threshold when guaranty inter-species phenotype distance is higher than the threshold value, certainly exists apparent difference between kind.Into When row approximation screening varieties, lower than the kind of the phenotype distance threshold as approximate kind.Method of the invention has the advantage that (1) fault-tolerance is strong, and preciseness is good.Even if individual character expression status observations are due to artificial origin (observed deviation, individual data Mistake) or kind and environment interaction, deviate normal value, approximate kind will not be omitted, i.e. the fault-tolerance of this method is stronger, mentions The high preciseness of approximate screening varieties.(2) with strong points.This method calculates application kind and approximate product by Database Systems The phenotype distance of kind, convenient for, by the plantation adjacent with application kind of closest kind, improving specific test when field trial Specific aim;(3) high-efficient.Using this method, do not need that each character is separately provided according to the expression status of application kind Expression status range shortens the approximate screening varieties time.
Detailed description of the invention
Fig. 1 is the approximate screening varieties result of peanut application kind 2011-0887A (mountain flower No. 9).
Specific embodiment
Embodiment 1: kind phenotype trait data library known to peanut is established
2004 to 2017, applicant carried out DUS test to the peanut varieties of Huang-Huai-Hai 388 parts times altogether.Each Two growth cycles of kind general test acquire data to all 31 characters in Testing Guideline in each period, adopt altogether Collect phenotype trait data more than 1.2 ten thousand.To the phenotype trait data of acquisition, according to test character expression way, data type into Arrangement is gone.On this basis, Huang-Huai-Hai peanut varieties phenotype trait data library is established, stores a product more than 300 altogether The DUS test character data of kind.The screening of this example approximation kind relies on above-mentioned phenotype trait data library to carry out.
According to the expression status observation of each phenotypic character by the degree and personnel's observed deviation of environmental influence Size, to all expression status code differences of selected rice phenotypic character, (such as code is 1-9, and difference 0,1,2 ... 8) Different weights is set, and the weight of each character is arranged as shown in table 2.1-31.2, is further arranged based on the table To one group of statistical data (table 1), above-mentioned phenotype trait data library is inputted.
1 florescence of character
Trait expression mode: QN;
Stability grade: 4 (generally 3-4 codes of fluctuation).
2.1 expression status of table and code
2.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 1 2 3 4 5 6
2 plant of character: flowering habit;
Trait expression mode: QL;
Stability grade: 2 (generally 2 codes of fluctuation).
3.1 expression status of table and code
Expression status Alternating is bloomed Continuously bloom
Code 1 2
3.2 code difference of table and weight
Code difference 0 1
Weight 0 6
3 stem of character: flowering habit;
Trait expression mode: QL;
Stability grade: 2 (generally 2 codes of fluctuation).
4.1 expression status of table and code
Expression status Nothing Alternating is bloomed Continuously bloom
Code 1 2 3
4.2 code difference of table and weight
Code difference 0 1 2
Weight 0 6 6
4 leaf of character: leaflet shape;
Trait expression mode: PQ;
Stability grade: 3 (general 2 codes).
5.1 expression status of table and code
Expression status Oblong Ellipse Obovate Wide obovate
Code 1 2 3 4
5.2 code difference of table and weight
Code difference 0 1 2 3
Weight 0 0 1 2
The flower of character 6: corolla color;
Trait expression mode: QN;
Stability grade: 1.
6.1 expression status of table and code
Expression status It is light yellow Yellow It is orange-yellow
Code 1 2 3
6.2 code difference of table and weight
Code difference 0 1 2
Weight 0 0 2
7 plant of character: habit;
Trait expression mode: QN;
Stability grade: 2 (generally 2 codes of fluctuation).
7.1 expression status of table and code
Expression status Uprightly Half is upright It crawls
Code 1 2 3
7.2 code difference of table and weight
Code difference 0 1 2
Weight 0 0 1
Character 8 is only applicable to kind of crawling: stem: habit;
Trait expression mode: QL;
Stability grade: 1.
8.1 expression status of table and code
Expression status Uprightly It is open and flat
Code 1 2
8.2 code difference of table and weight
Code difference 0 1
Weight 0 4
Character 9 is only applicable to kind of crawling: side shoot: top upwarps degree;
Trait expression mode: QN;
Stability grade: 3 (general 3 codes).
9.1 expression status of table and code
9.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 1 2 3 4 5 6 7
10 leaf of character: leaflet size;
Trait expression mode: QN;
Stability grade: 4 (generally 4 codes of fluctuation).
10.1 expression status of table and code
10.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 0 1 2 3 4 5
11 stem of character: anthocyanin colour developing;
Trait expression mode: QL;
Stability grade: 1.
11.1 expression status of table and code
Expression status Nothing Have
Code 1 2
11.2 code difference of table and weight
Code difference 0 1
Weight 0 4
12 stem of character: density of pubescent;
Trait expression mode: QN;
Stability grade: 4 (generally 4 codes of fluctuation).
12.1 expression status of table and code
12.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 1 2 3 4 5 6
13 plant of character: stem height;
Trait expression mode: QN;
Stability grade: 3 (generally 3 codes of fluctuation).
13.1 expression status of table and code
13.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 1 2 3 4 5 6
14 plant of character: branch quantity;
Trait expression mode: QN;
Stability grade: 4 (can up to 4 codes).
14.1 expression status of table and code
14.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 1 2 3 4 5 6
15 plant of character: side shoot length;
Trait expression mode: QN;
Stability grade: 4 (general 4 codes).
15.1 expression status of table and code
15.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 1 2 3 4 5 6
16 maturity period of character
Trait expression mode: QN;
Stability grade: 3 (generally 3 codes of fluctuation).
16.1 expression status of table and code
16.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 1 2 3 4 5 6
17 pod of character: seed benevolence rate;
Trait expression mode: QN;
Stability grade: 3 (main 3 codes).
17.1 expression status of table and code
Expression status 1 to 2 2 to 3 3 or more
Code 1 2 3
17.2 code difference of table and weight
Code difference 0 1 2
Weight 0 0 1
18 plant of character: pod number;
Trait expression mode: QN;
Stability grade: 4 (can up to 4 codes).
18.1 expression status of table and code
18.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 1 2 3 4 5 6
19 seed benevolence of character: dormant period;
Trait expression mode: QN;
Stability grade: 4 (can up to 5 codes).
19.1 expression status of table and code
19.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 1 2 3 4 5 6
20 pod of character: length;
Trait expression mode: QN;
Stability grade: 4 (generally 4 codes of fluctuation).
20.1 expression status of table and code
20.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 1 2 3 4 5 6
21 pod of character: contracting degree of hanging;
Trait expression mode: QN;
Stability grade: 4 (generally 4 codes of fluctuation).
21.1 expression status of table and code
21.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 1 2 3 4 5 6
22 pod of character: fruit mouth obvious degree;
Trait expression mode: QN;
Stability grade: 4 (generally 4 codes of fluctuation).
22.1 expression status of table and code
22.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 1 2 3 4 5 6
Character 23 has been only applicable to fruit mouth kind: pod: fruit mouth shape;
Trait expression mode: QL;
Stability grade: 1.
23.1 expression status of table and code
Expression status Directly It is curved
Code 1 2
23.2 code difference of table and weight
Code difference 0 1
Weight 0 2
Character 24: pod: surface texturisation;
Trait expression mode: PQ;
Stability grade: 3 (generally 2 codes of fluctuation).
24.1 expression status of table and code
Expression status It is smooth It is smooth in In In to coarse It is coarse
Code 1 2 3 4 5
24.2 code difference of table and weight
Code difference 0 1 2 3 4
Weight 0 0 1 2 3
Character 25: pod: kernel percent;
Trait expression mode: QN;
Stability grade: 4 (generally 3 codes of fluctuation).
25.1 expression status of table and code
25.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 1 2 3 4 5 6
Character 26:* seed benevolence: hundred benevolence weights;
Trait expression mode: QN;
Stability grade: 3 (generally 3 codes of fluctuation).
26.1 expression status of table and code
26.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 1 2 3 4 5 6
27 seed benevolence of character: shape;
Trait expression mode: PQ;
Stability grade: 2 (generally 2 codes of fluctuation).
27.1 expression status of table and code
Expression status It is spherical Cylindricality Taper
Code 1 2 3
27.2 code difference of table and weight
Code difference 0 1 2
Weight 0 0 2
Character 28: seed benevolence: kernel seed coat colour quantity;
Trait expression mode: QL;
Stability grade: 1.
28.1 expression status of table and code
Expression status It is monochromatic Polychrome
Code 1 2
28.2 code difference of table and weight
Code difference 0 1
Weight 0 4
Character 29: it is only applicable to monochromatic kind of skin kind: seed benevolence: kernel seed coat colour;
Trait expression mode: PQ;
Stability grade: 2 (generally 2 codes of fluctuation).
29.1 expression status of table and code
Expression status White It is light brown Brown Light red Peony Purple Darkviolet Black
Code 1 2 3 4 5 6 7 8
29.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7
Weight 0 0 4 4 4 4 4 4
Character 30: seed benevolence: kind skin crackle;
Trait expression mode: QN;
Stability grade: 4 (generally 4 codes of fluctuation).
30.1 expression status of table and code
30.2 code difference of table and weight
Code difference 0 1 2 3 4 5 6 7 8
Weight 0 0 0 1 2 3 4 5 6
Character 31: seed benevolence: intradermal surface color is planted;
Trait expression mode: QN;
Stability grade: 2 (generally 2 codes of fluctuation).
31.1 expression status of table and code
Expression status White It is light yellow Buff
Code 1 2 3
31.2 code difference of table and weight
Code difference 0 1 2
Weight 0 0 2
Embodiment 2: the screening of peanut approximation kind
1. peanut application kind totally 4, code name and title are as shown in table 32;
2. application kind is passed through each trait data (table 33) of the acquisition of plantation in 1 year, importing peanut phenotype trait data library;
3. setting 2 for safe distance, " screening " button is clicked, Database Systems list the close of corresponding each application kind Like kind inventory (table 34, Fig. 1).Table 34 only lists the approximate kind that phenotype distance is 0.
Table 32 applies for kind
Serial number Apply for kind number Apply for variety name
1 2011-0887A Mountain flower No. 9
2 2011-0888A Mountain flower No. 11
3 2011-0889A Mountain flower No. 12
4 2012-0416A Agricultural university 056
Table 33 applies for the data (expression status of each character, i.e. code value) of kind First Year acquisition
The part approximation kind that table 34 filters out
This method automatically can be lined up selected approximate kind according to phenotype by Database Systems apart from size, be convenient for By the plantation adjacent with application kind of closest kind when field trial, the specific aim of specific test is improved.

Claims (2)

1.一种基于表型距离的花生特异性测试近似品种筛选方法,其特征是,1. a kind of peanut specificity test based on phenotypic distance approximate variety screening method, it is characterized in that, (1)设置各个花生表型性状代码差值的权重(1) Set the weight of each peanut phenotypic trait code difference 采集当前仍具有应用潜力的花生品种,对采集的表型性状数据进行整理,建立花生品种表型性状数据库;根据各个表型性状的表达状态观测值受环境条件影响的程度和人员观测偏差的大小,对各个花生表型性状的所有表达状态的代码差值设置不同的权重,得到如表1所示的数据,输入花生品种表型性状数据库;Collect peanut varieties that still have application potential, organize the collected phenotypic trait data, and establish a peanut variety phenotypic trait database. , set different weights for the code differences of all expression states of each peanut phenotypic trait, obtain the data shown in Table 1, and input it into the peanut variety phenotypic trait database; 表1Table 1 (2)表型距离阈值的设定(2) Setting of phenotypic distance threshold 对成对花生品种的表型性状表达状态代码差值,按步骤(1)的方法赋予权重,所有表型性状上的权重相加得出两品种间的表型距离;将大量表型距离与品种间差异的明显程度进行比较,确定一个表型距离阈值,并将其输入花生品种表型性状数据库;For the difference between the expression status codes of the phenotypic traits of the paired peanut varieties, weights are assigned according to the method in step (1), and the weights on all phenotypic traits are added to obtain the phenotypic distance between the two varieties; Compare the obvious degree of differences between varieties, determine a phenotypic distance threshold, and input it into the peanut variety phenotypic trait database; (3)近似品种筛选(3) Screening of similar varieties 基于建立的花生品种表型性状数据库,开发基于表型距离的近似品种筛选软件;向花生品种表型性状数据库中输入待测的申请品种的表型性状数据,该筛选软件能根据步骤(2)的方法计算待测的申请品种与每个已知品种的表型距离,并与表型距离阈值进行比较,表型距离小于阈值的已知品种作为该待测的申请品种的近似品种。Based on the established peanut variety phenotypic trait database, develop approximate variety screening software based on phenotypic distance; input the phenotypic trait data of the application variety to be tested into the peanut variety phenotypic trait database, the screening software can be based on step (2) The method calculates the phenotypic distance between the application variety to be tested and each known variety, and compares it with the phenotypic distance threshold, and the known variety whose phenotypic distance is smaller than the threshold value is regarded as the approximate variety of the application variety to be tested. 2.如权利要求1所述的一种基于表型距离的花生特异性测试近似品种筛选方法,其特征是,所述步骤(2)的表型距离阈值为2,两品种间的表型距离<2时判定为近似品种。2. a kind of peanut specificity testing approximate variety screening method based on phenotypic distance as claimed in claim 1, is characterized in that, the phenotypic distance threshold value of described step (2) is 2, the phenotypic distance between two varieties When <2, it is judged as a similar variety.
CN201811462637.7A 2018-12-03 2018-12-03 A kind of peanut specific test approximation method for screening varieties based on phenotype distance Withdrawn CN109599150A (en)

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CN115836644A (en) * 2022-12-31 2023-03-24 浙江万里学院 Screening method and application of azalea hybrid progeny flower phenotype excellent single plant

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