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CN109549615A - The device and method of oxygen saturation endoscope monitoring optical dynamic therapy effect - Google Patents

The device and method of oxygen saturation endoscope monitoring optical dynamic therapy effect Download PDF

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CN109549615A
CN109549615A CN201811439213.9A CN201811439213A CN109549615A CN 109549615 A CN109549615 A CN 109549615A CN 201811439213 A CN201811439213 A CN 201811439213A CN 109549615 A CN109549615 A CN 109549615A
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light source
oxygen
wavelength
endoscope
oxygen saturation
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李江华
苏美英
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Shenzhen Institute of Information Technology
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/04—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0073—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by tomography, i.e. reconstruction of 3D images from 2D projections
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
    • A61B5/0084—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
    • A61B5/0086—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters using infrared radiation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue
    • A61B5/1455—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue using optical sensors, e.g. spectral photometrical oximeters
    • A61B5/14551—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue using optical sensors, e.g. spectral photometrical oximeters for measuring blood gases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue
    • A61B5/1455—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue using optical sensors, e.g. spectral photometrical oximeters
    • A61B5/1459—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue using optical sensors, e.g. spectral photometrical oximeters invasive, e.g. introduced into the body by a catheter
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/48—Other medical applications
    • A61B5/4836—Diagnosis combined with treatment in closed-loop systems or methods
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/48—Other medical applications
    • A61B5/4848—Monitoring or testing the effects of treatment, e.g. of medication
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00—Radiation therapy
    • A61N5/06—Radiation therapy using light
    • A61N5/0613—Apparatus adapted for a specific treatment
    • A61N5/062—Photodynamic therapy, i.e. excitation of an agent
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00—Radiation therapy
    • A61N5/06—Radiation therapy using light
    • A61N5/067—Radiation therapy using light using laser light

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  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)

Abstract

The present invention relates to a kind of device and method of oxygen saturation endoscope monitoring optical dynamic therapy effect, comprising: in advance after injection photosensitizer to destination organization；Destination organization is irradiated using the light source that endoscope imports, function or metamorphosis occur for induction destination organization, and then lead to cellular damage and necrosis effect, as optical dynamic therapy, the participation that this effect must be aerobic.Therefore, the oxygen distribution image by comparison destination organization before and after photodynamic action quantitatively calculates the variation of each point oxygen saturation value to reflect the variation of oxygen metabolism in fine vascular in therapeutic process, to monitor optical dynamic therapy effect in real time.

Description

The device and method of oxygen saturation endoscope monitoring optical dynamic therapy effect
Technical field
The present invention relates to medicine and medical instrument.The specifically device of oxygen saturation endoscope monitoring optical dynamic therapy effect And method.
Background technique
Optical dynamic therapy (PDT) acts on the selectivity intake and retention of photosensitizer using sick cell, is allowed to certain It is more in time to retain in pathological tissues, at this time with the laser irradiation lesions position of specific wavelength, divide in biological tissues Under the participation of sub- oxygen, strong photochemical reaction can be induced, to destroy blood vessel and inhibit new vessels, to reach treatment mesh 's.PDT has the function of that tissue selectivity is good, toxic side effect is low, treatment time is short, can protect the features such as appearance and vitals, Recently as its reaching its maturity technically, it is widely used to tumour, skin disease, inflammation, ophthalmology disease and infection class Disease etc..In recent years, near infrared spectrum (NIRS) method realizes noninvasive, the continuous monitoring of blood oxygen saturation in tissue.When Angiocarpy beating it is faint even with extracorporal circulatory system when, pulse oximetry non-metering, but tissue oxygenation non-destructive monitoring is still Information can be provided.Therefore, near infrared light blood oxygen detection method has been used to monitoring brain, muscle, flap, the local organizations such as tumour Blood transports situation.With NIRS measurement oxygen saturation rather than blood oxygen saturation can greatly reduce cost；Oxygen content is important in PDT Parameter, therefore, with NIRS detection PDT therapeutic effect be an ideal selection.And oxygen is measured with endoscope made of this Saturation degree is sensitive, quick come the device performance for monitoring optical dynamic therapy effect, at low cost, noninvasive, quantitative, continuous, excellent in real time Point.
Summary of the invention
It is an object of the invention in view of the deficienciess of the prior art, to provide a kind of oxygen saturation endoscope monitoring light dynamic The device and method of power therapeutic effect.The characteristics of being distributed using the oxygen content of oxygen saturation endoscopic technique measurement destination organization, By the damage effect that the variation of oxygen content distribution and oxygen saturation value before and after optical dynamic therapy is assessed to blood vessel.It is operated Performance sensitive, quick, expense is low, can accurately monitor optical dynamic therapy effect in real time.
The present invention is monitored for destination organization in its optical dynamic therapy effect of research；Moreover, the present invention is not limited only to this, also It can be used for the organism of other monitoring oxygen contents.
Another object of the present invention is to provide a kind of endoscope apparatus for realizing the above method.
The method of oxygen saturation endoscope of the present invention monitoring optical dynamic therapy effect include: had accumulated in laser irradiation it is photosensitive During the destination organization of agent carries out optical dynamic therapy, oxygen distribution image of the destination organization before and after photodynamic action is compared, Reflect the change of destination organization by the quantitative change for calculating oxygen saturation numerical value, to monitor the effect of optical dynamic therapy in real time Fruit.
Further, in advance after injection photosensitizer to destination organization；Endoscope import laser irradiation destination organization into Row optical dynamic therapy compares oxygen distribution image of the destination organization before and after photodynamic action, passes through quantitative calculating each point oxygen saturation The variation of angle value is to reflect the changes in oxygen metabolism of capilary in therapeutic process, to monitor target optical dynamic therapy effect in real time.
Further, it is placed in target tissue surface with interior snooping head, destination organization is carried out with dual wavelength near-infrared light source Tomoscan closes the principle with reduced hemoglobin in the difference measurement oxygen saturation of near infrared absorbing coefficient using oxygen, and use is black White high sensitivity Near Infrared CCD video camera shoots the blood distribution figure of available destination organization.
Further, comprising the following steps:
Step s100: before optical dynamic therapy, by the destination organization of endoscope position tissue, before scanning obtains optical dynamic therapy The oxygen distribution image of destination organization and corresponding quantitative oxygen saturation value；
Step s200 carries out optical dynamic therapy: i.e. injection photosensitizer, with corresponding photosensitizer after photosensitizer concentrates on destination organization The light source of absorbing wavelength irradiates, and makes destination organization that thermal response and irreversible chemical reaction occur；
Step s300, in optical dynamic therapy irradiation process, continuous scanning obtains the oxygen distribution of destination organization when optical dynamic therapy Image and corresponding quantitative oxygen saturation value；
Step s400 continues to scan on to obtain the oxygen distribution image of destination organization after optical dynamic therapy after optical dynamic therapy And corresponding quantitative oxygen saturation value；
Step s500, oxygen distribution image and corresponding quantitative oxygen saturation value to the destination organization of acquisition carry out data processing, The effect of optical dynamic therapy is assessed by the comparison of oxygen metabolism in pretherapy and post-treatment capilary.
Further, in the step s100 and step s400 light source assembly use oxygen saturation endoscope light source；Institute Step s200 light source is stated using photodynamic therapy light source；The step s300 uses two kinds of light sources simultaneously.
The photodynamic therapy light source and double-wavelength light source are confocal；Photodynamic therapy light source depends on the absorption of photosensitizer Peak；Double-wavelength light source is respectively 760nm and 850nm；If the absorption peak of photosensitizer is between 760nm and 850nm, double Wavelength light source may act as photodynamic therapy light source.Two kinds of light sources are LED light fibre output light source；What light source front end was placed synchronizes list There are two circular hole on wavelength filter switch, double-wavelength light source is emitted from different circular holes, scans positioned destination organization； Light source is connected to probe by optical fiber；Optical power density is up to 0.1 ~ 1W/cm when irradiation at short distance focal area2Magnitude
Further, the scanning process in the step s100, step s300 and step s400 is the control system by computer System software control synchronous control system；The light source of synchronous control system control light source transmitting is taken the photograph with black and white near-infrared high sensitivity CCD Camera is synchronous, when the light source of light source transmitting passes through a certain optical filter, via the trigger signal that synchronous control system is fed back, computer Control System Software be connected to after the trigger signal of synchronous control system to black and white near-infrared high sensitivity CCD camera shooting instruction And complete automatically scanning process.
Further, the collection process in the step s100, step s300 and step s400 be by data collecting card without The optical imagery of distortion ground acquisition near-infrared high sensitivity CCD camera photographic subjects tumor tissues, and vision signal is converted into Digital signal is simultaneously transmitted to computer；Image processing software algorithm realization in computer highlights tiny blood vessels and focal zone Domain.
Further, the quantitative scoring of oxygen content distribution map and oxygen content passes through in computer at last in the step s500 Image processing software independently derives the calculating of oxygen content according to improved Lambertian-Beer law and absorbance adduction law Formula, oxygen imaging diagnosis system show the vascular distribution figure of tissue and quantitatively provide local oxygen content.
Further, a kind of device for realizing the above method, including light source assembly, Single wavelength optical-filter switcher, optics Coupler, interior snooping head assembly, near-infrared high sensitivity CCD camera, signal acquisition component, computer and synchronous control system； The signal acquisition component, control assembly and computer are successively electrically connected；The Single wavelength optical-filter switcher is arranged in institute State the front end of light source assembly；There are two circular holes on the Single wavelength optical-filter switcher, are respectively the window that 760nm wavelength passes through The window that mouth and 850nm wavelength pass through；In this way, Single wavelength optical-filter switcher only passes through a kind of wavelength within the single time, it is real Existing Single wavelength imaging；
The interior snooping head assembly includes interior snooping head and probe-protecting device, before probe-protecting device is installed on interior snooping head End；The interior snooping head includes that the optical fiber of a branch of transmission light source and multi beam collect the optical fiber for scattering light；The outermost of interior snooping head It is the conduit to shield；The output end of the optical fiber of the transmission light source is spherical expansion tip；
The optical coupler is installed between Single wavelength optical-filter switcher and interior snooping head assembly；Light source assembly issues double wave Long near infrared light is emitted from Single wavelength optical-filter switcher, and optically coupled device enters the optical fiber of transmission light source, then through transmitting The scattering light of the output end Scan orientation destination organization of the optical fiber of light source, destination organization excitation is collected the optical fiber of scattering light by multi beam It collects；
Synchronous control system in light source assembly light source and near-infrared high sensitivity CCD camera be connected with conducting wire or data line； Meanwhile synchronous control system is connected with computer.
The optical fiber that the multi beam collects scattering light is connected by optical coupler with near-infrared high sensitivity CCD camera； The near-infrared high sensitivity CCD camera is electrically connected with data collecting card, and the data collecting card is connect with computer；
Control System Software and image processing software are installed in the computer；Described image processing software includes that oxygen imaging is examined Disconnected system；The oxygen imaging diagnosis system includes digital image acquisition system, oxygen imaging algorithm system, oxygen value assistant analysis system System.
Further, the light source in the light source assembly includes the light of photodynamic therapy light source and oxygen saturation endoscope Source；The light source of the oxygen saturation endoscope is dual wavelength near infrared light, and dual wavelength near infrared light includes 760nm near infrared light With 850nm near infrared light；The light source of optical dynamic therapy in light source assembly depends on the absorbing wavelength of photosensitizer, when it is positioned at double When the region of wavelength light source 760nm and 850nm, the light source of photodynamic therapy light source and oxygen saturation endoscope is used in conjunction with same Light source；Light source in the light source assembly is LED light source.
Further, the synchronous control system is made of single-chip microcontroller and relay, and single-chip microcontroller controls relay, relay Duration and the transfer sequence of light source assembly transmitting light source are controlled, while single-chip microcontroller controls probe assembly, control near-infrared is high The scattering light that sensitivity CCD camera energy sync pulse jamming is excited to Single wavelength each time.
The present invention compared with prior art have following advantages and effects
(1) high-resolution of the present invention.
(2) present invention monitors the numerical value change of tissue oxygenation saturation using oxygen saturation technology, with traditional venous oxygen Saturation degree monitoring, is a kind of undamaged monitoring technology, is capable of providing and monitors in body.
(3) the method for the present invention can directly utilize the absolute value of oxygen saturation quantitative expression oxygen content, so can accomplish The degree of impairment of the accurate optical dynamic therapy process medium vessels of monitoring in real time.
(4) in the present invention light source selected be both optical dynamic therapy simultaneously and the light source of oxygen content monitoring, it is possible to it is real Integration is now treated and monitored, provides a kind of more convenient and fast method for clinical research.
(5) for the device of the invention using the oxygen calculation formula simplified, the cost of each component is lower, especially uses LED light Source combination, so the cost of single unit system is also relatively low, is easy to application.
Detailed description of the invention
Fig. 1 is the structural schematic diagram that oxygen saturation endoscope monitors optical dynamic therapy effects device.
Fig. 2 is the interior snooping head cross-sectional view that oxygen saturation endoscope monitors optical dynamic therapy effect.
Fig. 3 oxygen saturation endoscope of the present invention monitors optical dynamic therapy effect imaging device work step figure.
Fig. 4 is the flow chart that oxygen saturation endoscope monitors optical dynamic therapy effect method.
In figure: 1, light source assembly；2, Single wavelength optical-filter switcher；3, the window that 760nm wavelength passes through；4,850nm wave The long window passed through；5, the optical fiber of transmission light source；6, the optical fiber of scattering light is collected；7, probe-protecting device；8, conduit；9, synchronous Control system；10, near-infrared high sensitivity CCD camera；11, signal acquisition component；12, computer；13, optical coupler.
Specific embodiment
The device of optical dynamic therapy effect is monitored to this oxygen saturation endoscope below with reference to specific example and attached drawing It is described in detail with the technical solution of method, so that it is clearer.
It please refers to shown in Fig. 1, the structure of oxygen saturation endoscope monitoring optical dynamic therapy effect imaging device of the present invention is shown It is intended to;Described device includes light source assembly 1, Single wavelength optical-filter switcher 2, optical coupler 13, interior snooping head assembly, close red Outer high sensitivity CCD camera 10, signal acquisition component 11, computer 12 and synchronous control system 9；The signal acquisition component, Control assembly is successively electrically connected with computer.
Light source in the light source assembly 1 includes the light source of photodynamic therapy light source and oxygen saturation endoscope；The oxygen The light source of saturation degree endoscope is dual wavelength near infrared light, and dual wavelength near infrared light includes 760nm near infrared light and 850nm close Infrared light；Light source in the light source assembly 1 is LED light source；
The front end of the light source assembly 1, the Single wavelength optical-filter switcher 2 is arranged in the Single wavelength optical-filter switcher 2 Upper there are two circular holes, are respectively the window 3 that 760nm wavelength passes through and the window 4 that 850nm wavelength passes through.
The interior snooping head assembly includes interior snooping head and probe-protecting device 7；Probe-protecting device 7 is installed on interior snooping The front end of head plays the role of spying upon head in protection；It please refers to shown in Fig. 2, the interior snooping head includes a branch of transmission light source Optical fiber 5 and multi beam collect the optical fiber 6 of scattering light；The outermost of interior snooping head is the conduit 8 to shield；The transmission light source Optical fiber 5 output end be spherical expansion tip.
The optical coupler 13 is installed between Single wavelength optical-filter switcher 2 and interior snooping head assembly；Light source assembly 1 Dual wavelength near infrared light is issued, dual wavelength near infrared light is emitted from two circular holes of Single wavelength optical-filter switcher 2, through optics coupling Clutch 13 enters the optical fiber 5 of transmission light source, then the output end Scan orientation destination organization of the optical fiber 5 through transmission light source, target The scattering light of excitation is organized to be collected by the optical fiber 6 that multi beam collects scattering light.
The synchronous control system 9 is made of single-chip microcontroller and relay, and single-chip microcontroller controls relay, and relay controls light source Component 1 emits duration and the transfer sequence of light source, while single-chip microcontroller controls probe assembly, controls near-infrared high sensitivity CCD The scattering light that 10 energy sync pulse jamming of video camera is excited to Single wavelength each time；Light source in synchronous control system 9 and light source assembly 1 It is connected with near-infrared high sensitivity CCD camera 10 with conducting wire or data line.Meanwhile synchronous control system 9 is connected with computer 12.
The optical fiber 6 that the multi beam collects scattering light passes through optical coupler 13 and near-infrared high sensitivity CCD camera 10 It is connected；The near-infrared high sensitivity CCD camera 10 is electrically connected with data collecting card 11, the data collecting card 11 and calculating Machine 12 connects；Data collecting card 11 acquires the telecommunications for each wavelength near infrared light that near-infrared high sensitivity CCD camera 10 is shot Number；Meanwhile the electric signal that near-infrared high sensitivity CCD camera 10 is shot is converted to digital signal and is sent to computer 12.
Control System Software and image processing software are installed in the computer 12.Described image processing software includes oxygen Imaging diagnosis system.Computer 12 controls synchronous control system 9 by Control System Software；Computer 10 is acquired by data The data image of each wavelength near infrared light needed for card 11 obtains oxygen imaging algorithm；Then, the number of each wavelength near infrared light According to image input oxygen imaging diagnosis system obtain each point containing oxygen value.
The oxygen imaging diagnosis system includes digital image acquisition system, oxygen imaging algorithm system, oxygen value assistant analysis system System.The optical imagery of each wavelength near infrared light needed for oxygen imaging algorithm can be obtained by digital image acquisition system.Number Image synthesis and image procossing are carried out according to image input oxygen imaging algorithm system, Pseudo Col ored Image is carried out to image later, it will be black The gray value of white image is mapped to corresponding colour.Oxygen value diagnostic system for doctor provide content and oxygen analysis function and to Quantitative result out.
Wherein, the light source of the optical dynamic therapy in light source assembly 1 depends on the absorbing wavelength of photosensitizer, when it is located at double wave When the region of long light source 760nm and 850nm, the light source of photodynamic therapy light source and oxygen saturation endoscope is used in conjunction with same light Source, i.e. selection dual wavelength near-infrared LED.
The near-infrared high sensitivity CCD camera 10 is black and white high sensitivity Near Infrared CCD video camera 10, is used to image group Knit the optical imagery of inner surface；Near-infrared high sensitivity CCD camera 10 uses high-definition camera.
The Single wavelength optical-filter switcher 2 is used to ensure in the single time only realize that unicast grows up to by a kind of wavelength Picture.
The data collecting card 11 is black and white high-definition image capture card, can without distortions be taken the photograph near-infrared high sensitivity CCD The video signal collection that camera 10 inputs guarantees the accuracy of raw image data to computer 12.
It please refers to shown in Fig. 3, when oxygen saturation endoscope monitoring optical dynamic therapy effect imaging device of the present invention works:
(1) initialization step realizes that two kinds of light sources are confocal and the synchronous operation of light source and camera.The unicast being sequentially connected is provided Long length filter switch 2, near-infrared high sensitivity CCD camera 10, data collecting card 11, oxygen imaging diagnosis system；
(2) destination organization for passing through endoscope position tissue, is shone with the light source of photodynamic therapy light source and oxygen saturation endoscope It penetrates；
(3) scanning step controls synchronous control system 9 by the Control System Software of computer.Synchronous control system 9 controls light The transmitting light source of source component 1 is synchronous with black and white near-infrared high sensitivity CCD camera 10, when a certain optical filter has light transmission, via The trigger signal that synchronous control system 9 is fed back, the Control System Software of computer 12 are connected to the trigger signal of synchronous control system 9 Automatic collection process is completed to 10 shooting instruction of black and white near-infrared high sensitivity CCD camera afterwards.Therefore, one acquisition process can To obtain the image data of several different Single wavelengths；
(4) video acquisition step, it is swollen that data collecting card 11 acquires 10 photographic subjects of near-infrared high sensitivity CCD camera without distortions The optical imagery of tumor tissue, and vision signal is converted into digital signal and is transmitted to computer 12；Image in computer 12 The realization of processing software algorithm highlights tiny blood vessels and focal area；
(5) the quantitative calculating of oxygen content distribution map and oxygen content.Image processing software in computer 12 is according to improved lambert Spy-Beer law and absorbance adduction law independently derive the calculation formula of oxygen content, oxygen imaging diagnosis system display group The vascular distribution figure knitted and quantitatively provide local oxygen content.
Above-mentioned oxygen saturation endoscope monitors photodynamic therapy effect method, passes through the target of endoscope position tissue Tissue, near-infrared high sensitivity CCD camera 10 acquire the organism optical image, provide video image, data collecting card 11 is nearly The vision signal that infrared high sensitivity CCD camera 10 inputs is converted into digital signal and is transmitted to computer 12, oxygen imaging diagnosis System is realized to full depth accurately image is organized, and the accuracy of detection is improved.
Before optical dynamic therapy, repeat step (1)-(5), obtain before optical dynamic therapy the oxygen distribution image of destination organization and Corresponding quantitative oxygen saturation value；
During optical dynamic therapy, injection photosensitizer repeats step (1)-after photosensitizer concentrates on destination organization first (5), obtain destination organization in optical dynamic therapy oxygen distribution image and corresponding quantitative oxygen saturation value；
After optical dynamic therapy, repeat step (1)-(5), obtain after optical dynamic therapy the oxygen distribution image of destination organization and Corresponding quantitative oxygen saturation value.
Fig. 4 oxygen saturation endoscope monitoring optical dynamic therapy effect method is please referred to, specifically includes the following steps:
Step s100: before optical dynamic therapy, scanning obtains before optical dynamic therapy the oxygen distribution image of destination organization and corresponding Quantitative oxygen saturation value；
Step s200 carries out optical dynamic therapy: i.e. injection photosensitizer, with corresponding photosensitizer after photosensitizer concentrates on destination organization The light source of absorbing wavelength irradiates, and makes destination organization that thermal response and irreversible chemical reaction occur；
Step s300, in optical dynamic therapy irradiation process, continuous scanning obtains the oxygen distribution of destination organization when optical dynamic therapy Image and corresponding quantitative oxygen saturation value；
Step s400 continues to scan on to obtain the oxygen distribution image of destination organization after optical dynamic therapy after optical dynamic therapy And corresponding quantitative oxygen saturation value；
Step s500, oxygen distribution image and corresponding quantitative oxygen saturation value to the destination organization of acquisition carry out data processing, The effect of optical dynamic therapy is assessed by the comparison of oxygen metabolism in pretherapy and post-treatment capilary.
In described step s100, s300 and s300, photodynamic therapy light source is confocal with double-wavelength light source；Optical dynamic therapy light Source depends on the absorption peak of photosensitizer；Double-wavelength light source is respectively 760nm and 850nm；If the absorption peak of photosensitizer between Between 760nm and 850nm, then double-wavelength light source may act as photodynamic therapy light source.Two kinds of light sources are LED light fibre output light Source.There are two circular hole on the synchronization Single wavelength optical-filter switcher that light source front end is placed, double-wavelength light source goes out from different circular holes It penetrates, scans positioned destination organization；Light source is connected to probe by optical fiber；Optical power density when irradiation at short distance focal area Up to 0.1 ~ 1W/cm2Magnitude.
In the step s100, control system is made of devices such as single-chip microcontroller and relays, and single-chip microcontroller controls relay, after Electrical equipment control light source duration and transfer sequence, while single-chip microcontroller control light source is synchronous with camera CCD.
In described step s100, s300 and s400, scanning step: under the action of control circuit, dual wavelength passes through unicast The successive irradiating sample of long length filter switch, while camera receives the sample signal of 760nm wavelength and the sample of 850nm wavelength respectively Product signal.
In the step s200, the absorbing wavelength of the photosensitizer is photodynamic therapy light source, and two kinds of technologies use simultaneously Diagnosing and treating integration may be implemented.
In described step s100, s300 and s400, the output end of output optical fibre described in component is peeped in the probe as spherical shape Extend tip.
In described step s100, s300 and s400, light source and CCD camera are run simultaneously in probe assembly, by single-chip microcontroller control System.
In described step s100, s300 and s400, acquisition system acquires the tissue light of inner surface by the CCD camera It learns image and is converted into digital signal, the digital data transmission is saved and shown with imaging unit to the control.
In described step s100, s300 and s400, data collecting card is connected to the rear end CCD, and acquisition digital signal simultaneously adopt by handle Image processing system of the digital data transmission collected to computer；
In described step s100, s300 and s400, the signal processing circuit of system include sync separator, adjust amplifier group, Synchronous integrator, sampling hold circuit, the circuit modules such as lock-in amplifier.
In the step s500, oxygen imaging algorithm system, oxygen value quantify computing system, thus obtain sample oxygen Butut with And local oxygen content value.
In the step s500, software calibration: the determination of system tentatively calibrated need to be adjusted by analyzing experimental data Section, the result of adjusting make the blood oxygen level of healthy individuals 76% or so.System further calibrates needs and a variety of method phases In conjunction with more person-times of measurement results compare, and obtain more accurate check formula and coefficient by regression analysis.
The device and method of the oxygen saturation endoscope monitoring optical dynamic therapy effect of invention described above can not have to Vascular contrast agent is the structure of display blood vessel, therefore to the mutual identification between blood vessel, lump, lymph node and blood vessel structure, there is it Distinctive feature.Its high resolution sensitively detects the metabolic alterations of morphological element's medium sized vein oxygen, can monitor optical dynamic therapy in time Effect.
One embodiment of the present invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.

Claims (10)

1.氧饱和度内窥镜监控光动力治疗效果的方法，其特征在于,预先注射光敏剂至目标组织后；在内窥镜导入的激光照射目标组织进行光动力治疗，对比目标组织在光动力作用前后的氧分布图像，通过定量计算各点氧饱和度值的变化来反映治疗过程中微血管的氧代谢变化，从而实时监控目标光动力治疗效果。1. the method for monitoring the effect of photodynamic therapy with oxygen saturation endoscope, it is characterized in that, after pre-injecting photosensitizer to the target tissue; the laser introduced by the endoscope irradiates the target tissue to carry out photodynamic therapy, and compares the target tissue in photodynamic therapy. The oxygen distribution images before and after the action reflect the changes in the oxygen metabolism of the microvessels during the treatment process by quantitatively calculating the change of the oxygen saturation value at each point, so as to monitor the effect of the target photodynamic therapy in real time. 2.氧饱和度内窥镜监控光动力治疗效果的方法，其特征在于,用内窥探头置于目标组织表面，用双波长近红外光源对目标组织进行断层扫描，利用氧合和还原血红蛋白在近红外吸光系数的差异测量氧饱和度的原理，用黑白高感度近红外CCD摄像机拍摄可以得到目标组织的血流分布图。2. A method for monitoring the effect of photodynamic therapy with an oxygen saturation endoscope, characterized in that an endoscope probe is placed on the surface of the target tissue, a dual-wavelength near-infrared light source is used to perform tomography on the target tissue, and oxygenated and reduced hemoglobin are used to The principle of measuring the oxygen saturation by the difference of the near-infrared absorption coefficient, the blood flow distribution map of the target tissue can be obtained by shooting with a black and white high-sensitivity near-infrared CCD camera. 3.根据权利要求1或2所述的方法，其特征在于，包括以下步骤：3. method according to claim 1 and 2, is characterized in that, comprises the following steps: 步骤s100：在光动力治疗前，通过内窥镜定位组织的目标组织，扫描得到光动力治疗前目标组织的氧分布图像及对应的定量氧饱和度值；Step s100: before photodynamic therapy, locate the target tissue of the tissue through an endoscope, and scan to obtain an oxygen distribution image of the target tissue before photodynamic therapy and a corresponding quantitative oxygen saturation value; 步骤s200，进行光动力治疗：即注射光敏剂，待光敏剂集中在目标组织后用相应光敏剂吸收波长的光源照射，使目标组织发生热反应和不可逆转的化学反应；Step s200, performing photodynamic therapy: that is, injecting a photosensitizer, after the photosensitizer is concentrated in the target tissue, irradiated with a light source having a wavelength absorbed by the corresponding photosensitizer, so that the target tissue undergoes a thermal reaction and an irreversible chemical reaction; 步骤s300，在光动力治疗照射过程中，连续扫描得到光动力治疗时目标组织的氧分布图像及对应的定量氧饱和度值；Step s300, in the photodynamic therapy irradiation process, continuously scan to obtain the oxygen distribution image of the target tissue during the photodynamic therapy and the corresponding quantitative oxygen saturation value; 步骤s400，在光动力治疗结束后，继续扫描得到光动力治疗后目标组织的氧分布图像及对应的定量氧饱和度值；Step s400, after the photodynamic therapy ends, continue to scan to obtain the oxygen distribution image of the target tissue after the photodynamic therapy and the corresponding quantitative oxygen saturation value; 步骤s500，对采集的目标组织的氧分布图像及对应的定量氧饱和度值进行数据处理，通过治疗前后微血管中氧代谢的对比来评估光动力治疗的效果。In step s500, data processing is performed on the collected oxygen distribution image of the target tissue and the corresponding quantitative oxygen saturation value, and the effect of the photodynamic therapy is evaluated by comparing the oxygen metabolism in the microvessels before and after the treatment. 4.根据权利要求3所述的方法，其特征在于，所述步骤s100和步骤s400中光源组件采用氧饱和度内窥镜的光源；所述步骤s200光源采用光动力治疗光源；所述步骤s300同时采用两种光源。4 . The method according to claim 3 , wherein in the steps s100 and s400 , the light source assembly adopts a light source of an oxygen saturation endoscope; in the step s200 , the light source adopts a photodynamic therapy light source; in the step s300 Two light sources are used at the same time. 5.根据权利要求3所述的方法，其特征在于，所述步骤s100、步骤s300和步骤s400中的扫描过程是通过计算机的控制系统软件控制同步控制系统；同步控制系统控制光源发射的光源与黑白近红外高感度CCD摄像机同步，光源发射的光源经过某一滤光片时，经由同步控制系统反馈的触发信号，计算机的控制系统软件接到同步控制系统的触发信号后给黑白近红外高感度CCD摄像机拍摄指令而完成自动扫描过程。5. The method according to claim 3, wherein the scanning process in the step s100, the step s300 and the step s400 is to control the synchronous control system through the control system software of the computer; the synchronous control system controls the light source emitted by the light source and The black and white near-infrared high-sensitivity CCD cameras are synchronized. When the light source emitted by the light source passes through a certain filter, the trigger signal fed back by the synchronous control system, the control system software of the computer receives the trigger signal of the synchronous control system and gives the black and white near-infrared high sensitivity. CCD camera shooting instructions to complete the automatic scanning process. 6.根据权利要求3所述的方法，其特征在于，所述步骤s100、步骤s300和步骤s400中的采集过程是由数据采集卡无失真地采集近红外高感度CCD摄像机拍摄目标肿瘤组织的光学图像，并把视频信号转化为数字信号并传送到计算机；计算机内的图像处理软件用算法实现凸显微小血管及病灶区域。6. The method according to claim 3, wherein the acquisition process in the step s100, the step s300 and the step s400 is to collect the optical data of the target tumor tissue by a near-infrared high-sensitivity CCD camera without distortion by the data acquisition card. image, and convert the video signal into a digital signal and send it to the computer; the image processing software in the computer uses algorithms to highlight tiny blood vessels and lesion areas. 7.根据权利要求3所述的方法，其特征在于，所述步骤s500中氧含量分布图和氧含量的定量计算是通过计算机中的图像处理软件根据改进的朗伯特-比尔定律和吸光度加和定律独立地推导出氧含量的计算公式，氧成像诊断系统显示组织的血管分布图和定量给出局部氧含量。7. The method according to claim 3, characterized in that, in the step s500, the oxygen content distribution map and the quantitative calculation of the oxygen content are based on the improved Lambert-Beer law and the absorbance addition by the image processing software in the computer. The formula for calculating the oxygen content is derived independently from the law and the oxygen imaging diagnostic system displays the vascular distribution map of the tissue and quantitatively gives the local oxygen content. 8.根据权利要求1或2所述的方法，一种实现权利要求1-3所述方法的装置，其特征在于，包括光源组件、单波长滤光片切换器、光学耦合器、内窥探头组件，近红外高感度CCD摄像机、信号采集组件、计算机和同步控制系统；所述信号采集组件、控制组件与计算机依次电气连接；所述单波长滤光片切换器设置在所述光源组件的前端；所述单波长滤光片切换器上有两个圆孔,其分别为760nm波长通过的窗口和850nm波长通过的窗口；这样，单波长滤光片切换器在单一时间内只通过一种波长，实现单波长成像；8. The method according to claim 1 or 2, a device for implementing the method according to claim 1-3, characterized in that it comprises a light source assembly, a single-wavelength filter switch, an optical coupler, an endoscope probe components, a near-infrared high-sensitivity CCD camera, a signal acquisition component, a computer and a synchronous control system; the signal acquisition component, the control component and the computer are electrically connected in sequence; the single-wavelength filter switcher is arranged at the front end of the light source component ; There are two circular holes on the single-wavelength filter switcher, which are respectively the window through which the wavelength of 760nm passes and the window through which the wavelength of 850nm passes; in this way, the single-wavelength filter switcher only passes one wavelength in a single time. , to achieve single-wavelength imaging; 所述内窥探头组件包括内窥探头和探头保护装置，探头保护装置安装于内窥探头的前端；所述内窥探头包括一束传输光源的光纤和多束收集散射光的光纤；内窥探头的最外围是起保护作用的导管；所述传输光源的光纤的输出端为球形扩展尖端；The endoscopic probe assembly includes an endoscopic probe and a probe protection device, and the probe protection device is installed at the front end of the endoscopic probe; the endoscopic probe includes a bundle of optical fibers for transmitting light sources and multiple bundles of optical fibers for collecting scattered light; the endoscopic probe The outermost periphery is a protective conduit; the output end of the optical fiber transmitting the light source is a spherical extension tip; 所述光学耦合器安装于单波长滤光片切换器和内窥探头组件之间；光源组件发出双波长近红外光从单波长滤光片切换器出射，经光学耦合器进入传输光源的光纤，然后经传输光源的光纤的输出端扫描定位目标组织，目标组织激发的散射光由多束收集散射光的光纤收集；The optical coupler is installed between the single-wavelength filter switcher and the endoscope probe assembly; the light source assembly emits dual-wavelength near-infrared light from the single-wavelength filter switcher, and enters the optical fiber of the transmission light source through the optical coupler, Then, the target tissue is scanned and positioned through the output end of the optical fiber of the transmission light source, and the scattered light excited by the target tissue is collected by multiple optical fibers that collect scattered light; 同步控制系统与光源组件中的光源和近红外高感度摄像机用导线或数据线相连；同时，同步控制系统与计算机相连；The synchronous control system is connected with the light source in the light source assembly and the near-infrared high-sensitivity camera by wires or data lines; at the same time, the synchronous control system is connected with the computer; 所述的多束收集散射光的光纤通过光学耦合器与近红外高感度CCD摄像机相连；所述近红外高感度CCD摄像机与数据采集卡电气相连，所述数据采集卡与计算机连接；The multiple optical fibers for collecting scattered light are connected with the near-infrared high-sensitivity CCD camera through an optical coupler; the near-infrared high-sensitivity CCD camera is electrically connected with a data acquisition card, and the data acquisition card is connected with a computer; 所述计算机内安装有控制系统软件和图像处理软件；所述图像处理软件包括氧成像诊断系统；所述氧成像诊断系统包括数据图像采集系统、氧成像算法系统、氧值辅助分析系统。Control system software and image processing software are installed in the computer; the image processing software includes an oxygen imaging diagnostic system; the oxygen imaging diagnostic system includes a data image acquisition system, an oxygen imaging algorithm system, and an oxygen value auxiliary analysis system. 9.根据权利要求8所述的装置，其特征在于，所述光源组件中的光源包括光动力治疗光源和氧饱和度内窥镜的光源；所述氧饱和度内窥镜的光源是双波长近红外光，双波长近红外光包括760nm 近红外光和850nm近红外光；光源组件中的光动力治疗的光源取决于光敏剂的吸收波长，当它位于双波长光源760nm和850nm的区域时，光动力治疗光源和氧饱和度内窥镜的光源共同使用同一光源；所述光源组件中的光源均为LED光源。9. The device according to claim 8, wherein the light source in the light source assembly comprises a photodynamic therapy light source and a light source of an oxygen saturation endoscope; the light source of the oxygen saturation endoscope is a dual-wavelength light source Near-infrared light, dual-wavelength near-infrared light includes 760nm near-infrared light and 850nm near-infrared light; the light source of photodynamic therapy in the light source assembly depends on the absorption wavelength of the photosensitizer, when it is located in the region of 760nm and 850nm of the dual-wavelength light source, The photodynamic therapy light source and the light source of the oxygen saturation endoscope share the same light source; the light sources in the light source assembly are all LED light sources. 10.根据权利要求8所述的装置，其特征在于，所述同步控制系统由单片机和继电器组成，单片机控制继电器，继电器控制光源组件发射光源的持续时间和切换顺序，同时单片机控制探头组件，控制近红外高感度CCD摄像机能同步拍摄到每一次单波长激发的散射光。10. The device according to claim 8, wherein the synchronous control system is composed of a single-chip microcomputer and a relay, the single-chip microcomputer controls the relay, and the relay controls the duration and switching sequence of the light source assembly emitting light source, while the single-chip microcomputer controls the probe assembly, controls the The near-infrared high-sensitivity CCD camera can simultaneously capture the scattered light excited by each single wavelength.
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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202960439U (en) * 2012-08-01 2013-06-05 深圳市雷迈科技有限公司 Multi-purpose light source for medical endoscope
CN103920248A (en) * 2014-04-29 2014-07-16 北京理工大学 Synchronous photodynamic therapy device
CN105263576A (en) * 2013-05-13 2016-01-20 荒井光医学研究所有限公司 Therapy-progress-level monitoring device and method
CN106073801A (en) * 2016-08-16 2016-11-09 深圳信息职业技术学院 A kind of external cavum nasopharyngeum vena systemica blood oxygen saturation formation method and device
JP2017000838A (en) * 2012-06-01 2017-01-05 ソニー株式会社 Medical device and control method
US20170224205A1 (en) * 2016-02-04 2017-08-10 Wright State University Light endoscope system for imaging, light delivery, and therapy response monitoring

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017000838A (en) * 2012-06-01 2017-01-05 ソニー株式会社 Medical device and control method
CN202960439U (en) * 2012-08-01 2013-06-05 深圳市雷迈科技有限公司 Multi-purpose light source for medical endoscope
CN105263576A (en) * 2013-05-13 2016-01-20 荒井光医学研究所有限公司 Therapy-progress-level monitoring device and method
CN103920248A (en) * 2014-04-29 2014-07-16 北京理工大学 Synchronous photodynamic therapy device
US20170224205A1 (en) * 2016-02-04 2017-08-10 Wright State University Light endoscope system for imaging, light delivery, and therapy response monitoring
CN106073801A (en) * 2016-08-16 2016-11-09 深圳信息职业技术学院 A kind of external cavum nasopharyngeum vena systemica blood oxygen saturation formation method and device

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