CN109414399B - Cosmetic composition for skin irritation reduction and skin inflammation reduction comprising hydrangea fermentation product - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin irritation reduction and skin inflammation reduction comprising fermented product of hydrangea macrophylla as an active ingredient. According to the present invention, the cosmetic composition comprising the fermented product of hydrangea macrophylla of the present invention as an active ingredient relieves skin irritation and skin inflammation by inhibiting the expression of inflammation-inducing cytokines (IL-6, IL-8, IL-1 β), and is thus finally effectively applicable to the use for preventing and improving skin allergy, improving redness, preventing and improving dermatitis, and the like.

Description

Skin irritation relief and skin inflammation relief cosmetic containing hydrangea fermented product combination

technical field

The present invention relates to a cosmetic composition for alleviating skin inflammation, and more particularly, to a cosmetic composition for alleviating skin irritation and skin inflammation comprising a hydrangea fermented product.

Background technique

One of the most important functions of the skin is its protective function as a barrier. The skin that is directly exposed to the external environment is the most important first line of defense against loss of body fluids and protects the body from the harmful environment. Also, perform a barrier function against toxic substances or microorganisms, physical stimuli or ultraviolet rays. However, if this skin barrier function is subjected to various external and internal stimuli of the skin, the skin barrier is damaged, resulting in dryness of the skin or an inflammatory reaction of the skin. At the same time, if the initial inflammatory reaction that occurs at this time cannot be effectively controlled, it may deteriorate into severe dermatitis.

However, due to the effects of population growth, urbanization, industrialization, and increase in automobiles, air pollution has become serious, and thus, the types of pollutants have become diverse. In particular, due to the location factor of South Korea, the damage of yellow sand and fine dust caused by the movement of air pollutants discharged from China has also become more serious. Particulate matter is classified into fine dust (PM10) with a diameter of 10 μm or less and ultrafine dust (PM2.5) with a diameter of 2.5 μm or less according to the size of the particles. The Korean Ministry of Environment has restricted PM10 as an air pollutant. It is known that such air pollutants such as fine dust induce respiratory diseases and heart diseases, and at the same time, the influence on the skin which occupies a wide area in the body is also attracting more and more attention. In particular, it is reported that fine dust in atmospheric pollutants impairs the function of the epidermal barrier and worsens atopic dermatitis. Moreover, compared with ordinary skin, sensitive skin reacts more sensitively to external stimuli or environmental changes, thereby showing symptoms of irritation or dermatitis. According to research, compared with normal people, people with this sensitive skin are more sensitive to the degree of stimulation of dust particles, and can feel more than three times the stimulation. Therefore, contaminants such as fine dust are highly likely to cause skin problems especially for those with sensitive skin. It also affects skin aging, and according to recent epidemiological studies, increased wrinkles and pigmentation in dust-exposed skin are directly related to aging. The fine dust produces reactive oxygen species to inhibit collagen synthesis, and can activate the decomposition. Polycyclic aromatic hydrocarbons (PAHs) adsorbed on the surface of the dust particles increase the decomposition of collagen through AhR activation of foreign body metabolism, thereby causing skin aging. Therefore, methods to reduce the direct contact between fine dust and skin and new therapeutic methods through molecular mechanism related research should be explored.

The inflammatory response in the skin is a defense against damage to the skin caused by physical stimulation, chemical substances, bacteria, and the like, and a variety of immune cells and inflammation-inducing cytokines are involved. Interleukin-6 (IL-6, interleukin-6), interleukin-8 (IL-8, interleukin-8), interleukin-1β (IL-1β, interleukin-β) are representative inflammations induce cytokines. IL-1β is a typical cytokine that mediates inflammatory responses formed by activated mononuclear phagocytes, epithelial cells, and vascular endothelial cells. IL-6 is secreted by T cells and macrophages, stimulates immune responses, and regulates Cytokines of inflammatory response. In addition, not only inflammation-inducing cytokines, but also activated macrophages overproduce nitric oxide (NO) or prostagladin E2 (PGE2), thereby further activating the inflammatory process. Pro- and anti-inflammatory dust particles have been reported to increase the expression of this inflammation-inducing cytokine in human keratinocytes.

In the past, various attempts have been made to develop cosmetics that can use natural extracts to impart anti-inflammatory functions to the skin. Typical examples are as follows: Korean Laid-Open Patent Publication No. 2007-0011939 proposes a cosmetic composition containing camellia extract, Korean Laid-Open Patent No. 2010-0086698 proposes a skin-soothing and irritation-relieving cosmetic composition containing mung bean fermentation-enzyme extract, and Korean Laid-Open Patent No. 2013-0016930 mentions that it contains sage, lemon balm, balsam Cosmetic composition for allergic skin and skin irritation relief containing cauliflower and onion extracts as active ingredients. In addition, Korean Laid-Open Patent No. 2015-0078948 proposes a cosmetic composition for improving skin inflammation or relieving skin irritation containing mixed extracts of mistletoe, pine bark, and tilapia as active ingredients, and Korean Laid-Open Patent No. 2009-0091547 Anti-inflammatory and skin irritation-relieving cosmetic compositions comprising mixed extracts of Eclipta and Cnidium are proposed. However, none of the above-mentioned cosmetic compositions describes the skin irritation alleviation and skin inflammation alleviation effects of the hydrangea fermented extract of the present invention.

In this regard, the present inventors have made diligent studies to overcome the above-mentioned problems of the prior art, and finally determined that the inflammatory cytokines increased by various stimuli, especially by the stimulation of fine dust, are related to skin irritation and skin inflammation, in order to explore and improve this problem. A comparison was made by screening plant extracts for inhibiting the expression of inflammatory cytokines, among which, the fermented product of fermented hydrangea extract was confirmed to have the ability to inhibit IL-6, an inflammation-inducing cytokine, and the like. The expression of IL-8 and IL-1β has the effect of relieving skin irritation and skin inflammation, thereby completing the present invention.

SUMMARY OF THE INVENTION

technical problem

Therefore, the main object of the present invention is to provide a cosmetic composition for relieving skin inflammation comprising a hydrangea fermented product, which has an inflammation-inducing cytokine by inhibiting the increase due to various stimuli, especially the stimulation of fine dust. The expression of IL-6, IL-8 and IL-1β can relieve skin irritation and skin inflammation.

In addition, another object of the present invention is to provide a cosmetic composition in which the hydrangea fermented product of the present invention is applied as a basic dosage form of a functional cosmetic composition to impart skin irritation relieving and skin inflammation relieving effects, thereby realizing skin allergy prevention. and improvement, flushing improvement, dermatitis prevention and improvement.

Technical solutions

According to one embodiment of the present invention, the present invention provides a cosmetic composition for alleviating skin irritation and skin inflammation, comprising a hydrangea fermented product as an active ingredient.

Hydrangea macrophylla (Hydrangea macrophylla), a deciduous shrub of the family Rosaceae, blooms from June to July, initially light purple, then sky blue, and finally light red. If the soil is neutral, the flowers are white, if the soil is acidic, the flowers are blue, and if the soil is alkaline, the flowers are pink. As a deciduous shrub, the ovate leaves are opposite, the margins are serrated, the length is 10-15cm, and the tip of the leaf is suddenly pointed. It is commonly known as a garden tree because of its prosperous flowers in early summer, but it is also known to be a Chinese herbal medicine for treating heart disease and an antipyretic. Mainly use whole grasses containing the roots. In addition, a whitening effect (Korean Laid-Open Patent No. 2014-0082555), a skin aging phenomenon improvement effect (Korean Laid-Open Patent No. 2009-0081178), and the like are well known. However, there is also no mention of the skin inflammation improving effect for external stimulation of the hydrangea fermented extract by pear blossom fermentation as in the present invention.

In this regard, the present inventors have confirmed that skin irritation and skin inflammation can be alleviated by suppressing the expression of inflammation-inducing cytokines by using a hydrangea fermented product, and completed the present invention.

The present invention is characterized in that the above-mentioned hydrangea fermented product is obtained by fermenting a hydrangea extract using pear koji.

The above-mentioned "using pear flower koji to ferment" refers to mixing pear flower koji and water for culturing. Specifically, it refers to mixing pear flower koji 10%, hydrangea extract 1%, and water 89% at a temperature of 25° C. Oxygen culture for 4 days.

The present invention is characterized in that the above-mentioned pear flower koji refers to rice koji, preferably prepared by inoculating a koji culture and a yeast liquid culture in a beat cake made of rice.

The above-mentioned koji culture is specifically a solid culture (Koji) obtained by inoculating, culturing and drying rice with Aspergillus oryzae after sterilizing rice adjusted to a moisture content of 38 to 42% at a temperature of 121°C for 30 minutes. . In addition, the yeast liquid culture (Saccharomyces cerevisiae) refers to a culture obtained by culturing yeast in a potato dextrose liquid medium.

The present invention is characterized in that the above-mentioned hydrangea extract can be extracted using any solvent used in the past, preferably, water, lower alcohols having 1 to 4 carbon atoms, butanediol, propylene glycol, glycerin or a mixed solvent thereof. to extract.

As the extraction method using the above-mentioned extraction solvent, common plant extraction methods such as hot water extraction, reflux cooling extraction, ultrasonic extraction, supercritical extraction and the like can be used. Specifically, hydrangea is crushed to prepare powder and immersed in Extraction at room temperature for 24 to 72 hours in the extraction solvent as described above.

The present invention is characterized in that the above-mentioned hydrangea fermented product is contained in an amount of 0.001 to 30 weight percent relative to the total weight of the cosmetic composition. If the hydrangea fermented product is less than 0.001 wt%, there is a concern that the effect of relieving skin inflammation is weak, which is not preferable. On the contrary, if the hydrangea fermented product exceeds 30 wt%, it is not preferable in terms of economy.

The present invention is characterized in that the above-mentioned hydrangea fermented product relieves skin irritation and skin inflammation by inhibiting inflammatory cytokines.

According to the experimental example of the present invention, the inhibitory effect of inflammatory cytokines increased by fine dust was confirmed. As a result, the hydrangea fermented product of the present invention exhibited an excellent inhibitory effect. The fermented hydrangea extract has a better inhibitory effect on skin inflammation. From this result, it can be seen that the hydrangea fermented product of the present invention not only relieves skin inflammation by inhibiting inflammation-inducing cytokines, but also relieves skin irritation such as fine dust.

Furthermore, the effect of relieving the irritation of the epidermis caused by fine dust was confirmed, and as a result, it was found that the fermented hydrangea of the present invention recovered the epidermis and epidermal cells damaged by the stimulation. From this result, it can be seen that the hydrangea fermented product can alleviate the damage of the epidermis caused by the irritation caused by the fine dust.

The present invention is characterized in that the cosmetic composition is selected from the group consisting of softening lotion, nutritional lotion, nutritional essence, nutritional oil, moisturizing oil, nutritional cream, pressed powder, facial mask, foundation, barrier cream, facial cleanser, shampoo, One or more dosage forms in the group consisting of lotions and ointments.

The ingredients contained in the cosmetic composition of the present invention may include, as active ingredients, various ingredients commonly used in cosmetic compositions, such as stabilizers, solubilizers, vitamins, pigments, and fragrances, in addition to the above-mentioned fermented products. conventional supplements and carriers.

effect of invention

As described above, the hydrangea fermented product of the present invention can effectively inhibit the expression of inflammation-inducing cytokines (IL-6, IL-8, IL-1β) that are overexpressed by various stimuli, especially dust, to relieve skin irritation and skin inflammation.

Description of drawings

FIG. 1 is a graph showing the results of Experimental Example 1 showing the degree of inhibition of IL-6 gene expression among inflammation-inducing cytokines.

FIG. 2 is a graph showing the results of Experimental Example 1 showing the degree of inhibition of IL-8 gene expression among inflammation-inducing cytokines.

FIG. 3 is a graph showing the results of Experimental Example 1 showing the degree of inhibition of IL-1β gene expression among inflammation-inducing cytokines.

Fig. 4 is a view showing a photograph of a measurement result of recovery of the epidermis damaged by fine dust when the hydrangea pear flower fermented extract was treated.

Detailed ways

Hereinafter, the present invention will be described in more detail by way of examples. These examples are only for illustrating the present invention, and thus the scope of the present invention is not to be construed as being limited to these examples.

Example 1: Preparation of Hydrangea Extract

After powdering the hydrangea, 100 g of each was extracted into a separate container, immersed in 1 L of a 70% by weight ethanol aqueous solution as an extraction solvent, and left at room temperature for 24 hours to obtain an extract. After filtering the obtained extract with filter paper, the filtrate was concentrated under reduced pressure with a rotary evaporative dryer to obtain an extract.

Example 2: Preparation of Hydrangea fermented product

The hydrangea fermented product was produced by fermenting the hydrangea extract obtained in the above Example 1 using pear flower koji. The present invention is characterized in that two kinds of inoculum, ie, a koji culture and a yeast liquid culture, are used for pear koji.

Koji culture refers to a solid culture (Koji) obtained by inoculating Aspergillus oryzae after sterilizing rice adjusted to a moisture content of 38 to 42% at 121° C. for 30 minutes and then culturing and drying it. Yeast liquid culture (Saccharomyces cerevisiae) refers to a culture in which yeast is cultured in a potato dextrose liquid medium.

The pear flower koji of the present invention is produced by the following method. First, 100 parts by weight of washed rice is dried at a temperature of 60° C. for 20 to 24 hours, and then pulverized. The powder that has passed through a sieve of about 40 meshes is collected, and water is added so that the moisture content becomes 40 to 45%, and then the powder is formed into a circular shape. After the round rice was reformed into a hole-shaped rice cake, it was steamed at a temperature of 100° C. for 1 hour. The two kinds of koji culture and yeast liquid culture cultured in advance were inoculated and mixed so as to be 1% of each weight part. At 25°C, aerobically cultivated for 4 days to complete the pear blossom koji.

The hydrangea pear flower fermented product is prepared by mixing 10% pear flower koji, 1% hydrangea flower extract, and 89% water, and anaerobic culture at 25°C for 4 days. The hydrangea pear flower fermented product is used in In the following experimental example 1.

Experimental Example 1: Evaluation of Anti-inflammatory Efficacy

In order to evaluate the anti-inflammatory effect of the fermented extract, IL-1β, IL-6, and IL-8, which are inflammation-inducing cytokines, were treated with fine dust (PM10), and their expression levels were measured. More specifically, HaCaT cells, which are keratinocytes, were treated with fine dust, and the final concentration of each raw material prepared in Example 1 and Example 2 was 10 μg/ml, and cultured for 4 hours. Next, mRNA was extracted from the cells, cDNA was synthesized, and a real-time polymerase chain reaction (PCR) was performed using a target template (primer), thereby finally evaluating the gene expression level of inflammatory cytokines. The results are shown in FIGS. 1 to 3 .

As a result, as shown in Fig. 1 to Fig. 3 , when each cytokine was induced by fine dust, the degree of inhibition by the hydrangea extract and the hydrangea fermented extract could be evaluated, and the pear fermented extract showed the highest level of all cytokines. and a significant induction inhibitory effect. Therefore, it was confirmed that the fermentation extract of the present invention is a very useful raw material for a cosmetic composition having a higher anti-inflammatory effect than before fermentation.

Experimental Example 2: Evaluation of Epidermal Irritation Relief Effect

In order to evaluate the effect of relieving skin epidermis irritation, three-dimensionally cultured artificial skin tissue was used to evaluate the relieving effect of the epidermal layer irritated and damaged by fine dust. 50 μg/ml of dust particles (PM10) were applied to the artificial skin surface, and the hydrangea extract and hydrangea pear flower fermented extract obtained in Example 1 and Example 2 were treated with a concentration of 10 μg/ml in the culture medium. thing. After additional culturing for 24 hours, after fixation in formalin, a paraffin block was prepared and H&E staining was performed. Thereby, the morphology of the stratum corneum and keratinocytes of the epidermis was measured. In addition, the measurement results are shown in FIG. 4 .

As a result, as shown in FIG. 4 , it was confirmed that the stratum corneum of the artificial skin tissue damaged by the dust treatment was relieved. Furthermore, it was confirmed that the density and shape of the keratinocytes in the base layer were restored. Therefore, it was confirmed that the fermentation extract of the present invention is a very useful raw material for a cosmetic composition having an irritation-relieving effect on an epidermal layer damaged by irritation.

Formulation Example 1: Preparation of Lotion

According to the composition components and composition ratios shown in Table 1 below, a lotion containing the fermented extract obtained in Example 2 above was prepared by a conventional method.

Table 1

Formulation Example 2: Preparation of Nutrient Essence

According to the composition components and composition ratios shown in Table 2 below, a moisturizing cream containing the fermented extract obtained in Example 2 was prepared by a conventional method.

Table 2

Formulation Example 3: Moisturizer

According to the compositional components and compositional ratios shown in Table 3 below, a moisturizing cream containing the fermented extract obtained in Example 2 was prepared by a conventional method.

table 3

Industrial Applicability

As described above, according to the present invention, the cosmetic composition comprising the hydrangea fermented product of the present invention as an active ingredient relieves skin irritation and skin irritation by inhibiting the expression of inflammation-inducing cytokines (IL-6, IL-8, IL-1β). Inflammation is relieved, and finally, it can be effectively used for the prevention and improvement of skin allergies, the improvement of flushing, the prevention and improvement of dermatitis, and the like.

references

(Patent Document 0001) KR 10-2007-0011939 A

(Patent Document 0002) KR 10-2010-0086698 A

(Patent Document 0003) KR 10-2013-0016930 A

(Patent Document 0004) KR 10-2015-0078948 A

(Patent Document 0005) KR 10-2009-0091547 A

Claims (6)

1. a cosmetic composition for skin irritation alleviation and skin inflammation alleviation, is characterized in that, comprises and utilizes pear blossom song to make the hydrangea pear flower fermented product obtained by fermenting the hydrangea flower extract as an active ingredient, and the hydrangea pear flower fermented The product is prepared by mixing 10% pear flower koji, 1% hydrangea extract, and 89% water at 25° C., and anaerobic culture for 4 days. 1.0% by weight, the pear flower koji is prepared by inoculating a koji culture and a yeast liquid culture in a rice cake made of rice. 2 . The cosmetic composition for relieving skin irritation and skin inflammation according to claim 1 , wherein the hydrangea extract is prepared by using water, a lower alcohol having a carbon number of 1 to 4 or a mixed solvent thereof. 3 . extract. 3 . The cosmetic composition for relieving skin irritation and skin inflammation according to claim 1 , wherein the hydrangea extract is extracted with water, butylene glycol, propylene glycol, glycerin or a mixed solvent thereof. 4 . 4. The cosmetic composition for relieving skin irritation and skin inflammation according to claim 1, wherein the fermented hydrangea pear flower ferments induces IL-6, IL-8, IL-1β of cytokines by inhibiting inflammation expression to relieve skin irritation and skin inflammation. 5 . The cosmetic composition for relieving skin irritation and skin inflammation according to claim 1 , wherein the hydrangea pear flower fermented product relieves skin irritation and skin inflammation by inhibiting inflammatory cytokines. 6 . 6. The cosmetic composition for alleviating skin irritation and skin inflammation according to claim 1, wherein the cosmetic composition for alleviating skin irritation and skin inflammation is selected from the group consisting of softening lotion, nutritional lotion, nutrition Serum, Nutrient Oil, Moisturizing Oil, Nutrient Cream, Pressed Powder, Mask, Foundation, Cream, Cleanser, Shampoo, Lotion, Ointment.

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