CN109371090A - A kind of process for extracting bread worm protein - Google Patents
A kind of process for extracting bread worm protein Download PDFInfo
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- CN109371090A CN109371090A CN201811591677.1A CN201811591677A CN109371090A CN 109371090 A CN109371090 A CN 109371090A CN 201811591677 A CN201811591677 A CN 201811591677A CN 109371090 A CN109371090 A CN 109371090A
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Abstract
The invention discloses a kind of high-quality process for extracting bread worm protein, include the following steps: that (1) carries out cleaning removal of impurities to yellow meal worm, sterilizing, drying, crushes to obtain yellow meal worm dry powder；(2) degreasing: dehydrated alcohol is added in yellow meal worm dry powder after the pre-treatment, is subsequently placed in the supercritical CO of tyre microwave generator2It is extracted in extraction equipment, the yellow meal worm dry powder after obtaining degreasing；(3) microwave just mentions: the yellow meal worm dry powder after degreasing is dry, NaOH solution is added later, is subsequently placed in progress microwave catalysis in micro-wave oven and obtains tenebrio molitor protein crude extract；(4) enzyme extraction: complex enzyme is added in tenebrio molitor protein crude extract obtained, carries out ultrasonic extraction, enzyme deactivation, obtains extracting solution of protein；(5) obtained extracting solution of protein is filtered, then dry high-quality tenebrio molitor protein.Extraction rate of protein prepared by extraction process of the invention is high, and fat content is low, and excellent quality, has a extensive future.
Description
Technical field
The invention belongs to the preparation technical fields of food protein, and in particular to a kind of extraction work of tenebrio molitor protein
Skill.
Background technique
Protein is the important component in food, is referred to as the first nutrient of the mankind or biogen, accounts for human body composition
45%, growth in humans development it is invariably closely related with protein, without protein just without life.In order to maintain mankind itself
Health, it is necessary to constantly from extraneous panning protein.It is reported that there are about 1/5 populations to lack protein in the world, especially it is a lack of
Animal protein.Protein resource shortage has been the world today, especially developing country's common problem.China is one
A populous nation, protein resource shortage situation are especially serious.Currently, China per capita in diet animal protein intake
It greatly differs from each other with world standard, just corresponds to 1/8-the 1/5 of developed country.With the continuous improvement of people's living standards,
People will be more and more big to the demand of high-quality high-grade animal protein, the conventional proteins food such as milk, egg, fish, meat
Demand of the people to protein will be far from satisfying.At that time, China will face serious protein resource crisis.Therefore, it seeks
Seek protein new resources, it has also become a urgent task of the pendulum in face of the mankind.
Insect is concerned as the living resources treasure-house not being fully utilized maximum on the earth.With science and technology
Development, people are increasingly distinctly understood that insect is fabulous protein resource.The type of insect is estimated at 2,000,000 kinds
Left and right, accounts for about 2/3 or more of entire animal kingdom's type, and the Known Species of plant are only 33.5 ten thousand kinds, and only the 1/3 of insect is left
It is right.The biomass of insect is big, can exceed that the total biomass of all animals, belong to renewable resource.And its food conversion
Rate is high, and reproduction speed is fast, and protein content is high, wherein determining edible up to more than 3650.Insect based food is fresh with its taste
The hot spots such as beauty, high protein, low fat, low in calories, Multivitamin, multiple trace element are favored by people.Some insects also contain
Special effective component has certain healthcare function, has very tempting development prospect.
Insect is concerned as the living resources treasure-house not being fully utilized maximum on the earth.With science and technology
Development, people are increasingly distinctly understood that insect is fabulous protein resource.The type of insect is estimated at 2,000,000 kinds
Left and right, accounts for about 2/3 or more of entire animal kingdom's type, and the Known Species of plant are only 33.5 ten thousand kinds, and only the 1/3 of insect is left
It is right.The biomass of insect is big, can exceed that the total biomass of all animals, belong to renewable resource.And its food conversion
Rate is high, and reproduction speed is fast, and protein content is high, wherein determining edible up to more than 3650.Insect based food is fresh with its taste
The hot spots such as beauty, high protein, low fat, low in calories, Multivitamin, multiple trace element are favored by people.Some insects also contain
Special effective component has certain healthcare function, has very tempting development prospect.
Summary of the invention
For overcome the deficiencies in the prior art, mentioning the purpose of the present invention is to provide a kind of high-quality tenebrio molitor protein
Taking technique, extraction process recovery rate is high, and extraction efficiency is high, and is capable of the nutritional ingredient of utmostly retaining protein, application
It has a extensive future.
To achieve the above object, the technical solution used in the present invention are as follows:
A kind of high-quality process for extracting bread worm protein, includes the following steps:
(1) pretreatment of yellow meal worm: yellow meal worm dry powder is obtained after carrying out cleaning removal of impurities, sterilizing, drying, crushing to yellow meal worm；
(2) degreasing: yellow meal worm dry powder quality 0.1- is accounted for by being added in pretreated yellow meal worm dry powder in step (1)
Then yellow meal worm dry powder is placed in the supercritical CO of tyre microwave generator by 0.2% dehydrated alcohol2In extraction equipment, micro-
Amplitude shines frequency 500-1500MHz, and temperature is 35-45 DEG C in extraction equipment, extracting pressure 30-35MPa, CO2Fluid flow
Yellow meal worm dry powder to extract 2-3h under conditions of 20-24L/h, after obtaining degreasing；
(3) microwave just mentions: the yellow meal worm dry powder after step (2) degreasing is dry, and it is 0.05- that concentration is added later
The NaOH solution of 0.1mol/L simultaneously stirs evenly, and will mix in the ratio of yellow meal worm dry powder and NaOH solution solid-liquid ratio 1:5-15g/mL
Conjunction object, which is placed in micro-wave oven, carries out microwave catalysis 60-240s up to tenebrio molitor protein crude extract；
(4) it enzyme extraction: is added after being about 7.0 by the adjusting of tenebrio molitor protein crude extract made from step (3) pH multiple
Synthase is heated to 55-65 DEG C later, and extracts 30-60min with 400-500W ultrasonic power, and enzyme deactivation later obtains Protein Extraction
Liquid；
(5) extracting solution of protein that step (4) obtains is filtered by coarse filter and is placed in ceramic micro filter film
It is filtered, ceramic micro filter film filters obtained filtrate and is filtered using ceramic super-filtering film, finally by ultrafiltration membrance filter
Resulting concentrate be spray-dried up to the high-quality tenebrio molitor protein.
Preferably, the moisture content of yellow meal worm dry powder is lower than 5% in step (1).
Preferably, the power in step (3) when microwave catalysis is 200-800W.
Preferably, complex enzyme described in step (4) is made of neutral proteinase and papain, neutral proteinase and wood
The mass ratio of melon protease is 1:1-1:2.
Preferably, enzyme deactivation described in step (4) is that the feed liquid after digesting is sent into enzyme deactivation device, slightly to 75-85 DEG C
Enzyme deactivation 4-8min is carried out afterwards.
Preferably, coarse filtration is filtered using filter cloth in step (5), and the average pore size of ceramic micro filter film is 200-
240nm, crossflow velocity 2-2.5m/s, the average pore size of ceramic super-filtering film are 20-30nm, crossflow velocity 2.5-3.5m/s.
Preferably, transmembrane pressure when ceramic micro filter film filters in step (5) is 0.15MPa, when ceramic super-filtering film filters
Transmembrane pressure be 0.1MPa.
Preferably, the inlet amount in step (5) when spray drying is 20ml/min, and the revolving speed of atomizer is 1000r/min.
In addition, the high-quality tenebrio molitor protein extracted by the extraction process is also claimed in the present invention.
Compared with prior art, the advantages and benefits of the present invention are:
(1) present invention substitutes simple supercritical extraction method using the method for microwave-assisted supercritical extract first and carries out
The separating and extracting of grease mainly improves grease by the penetrability of microwave irradiation, high frequency and is separated from yellow meal worm
Effect and efficiency, to improve the DNA purity of tenebrio molitor protein；
(2) present invention is just mentioned using microwave and ultrasonic enzyme mentions the extraction that the method combined carries out protein, one side energy
Greatly improve the efficiency extracted, it is often more important that two methods combine the extraction that can improve protein to the greatest extent
Rate obtains the maximum amount of protein extract；
(3) method that the present invention is combined using coarse filtration, ceramic micro filter and Ceramic excessive filtration three, can effectively remove de-etiolation
The ingredients such as impurity, ash content, grease in mealworm protein, to obtain most pure tenebrio molitor protein.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Embodiment 1
A kind of high-quality process for extracting bread worm protein, includes the following steps:
(1) pretreatment of yellow meal worm: to yellow meal worm carry out after cleaning removal of impurities, sterilizing, drying, crushing water content is lower than
5% yellow meal worm dry powder；
(2) degreasing: yellow meal worm dry powder quality 0.1% is accounted for by being added in pretreated yellow meal worm dry powder in step (1)
Dehydrated alcohol, then yellow meal worm dry powder is placed in the supercritical CO of tyre microwave generator2In extraction equipment, in microwave irradiation
Frequency 600MHz, temperature is 40 DEG C in extraction equipment, extracting pressure 30MPa, CO2Fluid flow extracts under conditions of being 20L/h
2h is taken, the yellow meal worm dry powder after obtaining degreasing；
(3) microwave just mentions: the yellow meal worm dry powder after step (2) degreasing is dry, and it is 0.07mol/L's that concentration is added later
NaOH solution simultaneously stirs evenly, and mixture is placed in microwave in the ratio of yellow meal worm dry powder and NaOH solution solid-liquid ratio 1:8g/mL
Microwave catalysis 90s is carried out in furnace up to tenebrio molitor protein crude extract；Wherein, power when microwave catalysis is 400W；
(4) enzyme extraction: by tenebrio molitor protein crude extract made from step (3) adjusting pH be about 7.0 after be added by
The complex enzyme of neutral proteinase and papain composition is heated to 55 DEG C later, and extracts 45min with 450W ultrasonic power,
The feed liquid after enzymatic hydrolysis is sent into enzyme deactivation device later, enzyme deactivation 5min is carried out after being warming up to 75 DEG C, obtains extracting solution of protein；Its
In, the mass ratio of neutral proteinase and papain is 1:1.5；
(5) extracting solution of protein that step (4) obtains is filtered to be placed in ceramic micro filter film by filter cloth and is carried out
Filtering, ceramic micro filter film filter obtained filtrate and are filtered using ceramic super-filtering film, finally will be obtained by ultrafiltration membrance filter
Concentrate be spray-dried up to the high-quality tenebrio molitor protein；Wherein, the average pore size of ceramic micro filter film is
The average pore size of 200nm, crossflow velocity 2m/s, transmembrane pressure 0.15MPa, ceramic super-filtering film are 25nm, and crossflow velocity is
2.5m/s, transmembrane pressure 0.1MPa.
Embodiment 2
A kind of high-quality process for extracting bread worm protein, includes the following steps:
(1) pretreatment of yellow meal worm: to yellow meal worm carry out after cleaning removal of impurities, sterilizing, drying, crushing water content is lower than
5% yellow meal worm dry powder；
(2) degreasing: yellow meal worm dry powder quality 0.1% is accounted for by being added in pretreated yellow meal worm dry powder in step (1)
Dehydrated alcohol, then yellow meal worm dry powder is placed in the supercritical CO of tyre microwave generator2In extraction equipment, in microwave irradiation
Frequency 600MHz, temperature is 40 DEG C in extraction equipment, extracting pressure 30MPa, CO2Fluid flow extracts under conditions of being 22L/h
2h is taken, the yellow meal worm dry powder after obtaining degreasing；
(3) microwave just mentions: the yellow meal worm dry powder after step (2) degreasing is dry, and it is 0.08mol/L's that concentration is added later
NaOH solution simultaneously stirs evenly, and mixture is placed in microwave in the ratio of yellow meal worm dry powder and NaOH solution solid-liquid ratio 1:10g/mL
Microwave catalysis 90s is carried out in furnace up to tenebrio molitor protein crude extract；Wherein, power when microwave catalysis is 400W；
(4) enzyme extraction: by tenebrio molitor protein crude extract made from step (3) adjusting pH be about 7.0 after be added by
The complex enzyme of neutral proteinase and papain composition is heated to 55 DEG C later, and extracts 45min with 450W ultrasonic power,
The feed liquid after enzymatic hydrolysis is sent into enzyme deactivation device later, enzyme deactivation 5min is carried out after being warming up to 75 DEG C, obtains extracting solution of protein；Its
In, the mass ratio of neutral proteinase and papain is 1:1.5；
(5) extracting solution of protein that step (4) obtains is filtered to be placed in ceramic micro filter film by filter cloth and is carried out
Filtering, ceramic micro filter film filter obtained filtrate and are filtered using ceramic super-filtering film, finally will be obtained by ultrafiltration membrance filter
Concentrate be spray-dried up to the high-quality tenebrio molitor protein；Wherein, the average pore size of ceramic micro filter film is
The average pore size of 200nm, crossflow velocity 2m/s, transmembrane pressure 0.15MPa, ceramic super-filtering film are 25nm, and crossflow velocity is
2.5m/s, transmembrane pressure 0.1MPa.
Embodiment 3
A kind of high-quality process for extracting bread worm protein, includes the following steps:
(1) pretreatment of yellow meal worm: to yellow meal worm carry out after cleaning removal of impurities, sterilizing, drying, crushing water content is lower than
5% yellow meal worm dry powder；
(2) degreasing: yellow meal worm dry powder quality 0.1% is accounted for by being added in pretreated yellow meal worm dry powder in step (1)
Dehydrated alcohol, then yellow meal worm dry powder is placed in the supercritical CO of tyre microwave generator2In extraction equipment, in microwave irradiation
Frequency 700MHz, temperature is 40 DEG C in extraction equipment, extracting pressure 32MPa, CO2Fluid flow extracts under conditions of being 20L/h
2h is taken, the yellow meal worm dry powder after obtaining degreasing；
(3) microwave just mentions: the yellow meal worm dry powder after step (2) degreasing is dry, and it is 0.06mol/L's that concentration is added later
NaOH solution simultaneously stirs evenly, and mixture is placed in microwave in the ratio of yellow meal worm dry powder and NaOH solution solid-liquid ratio 1:9g/mL
Microwave catalysis 90s is carried out in furnace up to tenebrio molitor protein crude extract；Wherein, power when microwave catalysis is 400W；
(4) enzyme extraction: by tenebrio molitor protein crude extract made from step (3) adjusting pH be about 7.0 after be added by
The complex enzyme of neutral proteinase and papain composition is heated to 55 DEG C later, and extracts 45min with 450W ultrasonic power,
The feed liquid after enzymatic hydrolysis is sent into enzyme deactivation device later, enzyme deactivation 5min is carried out after being warming up to 75 DEG C, obtains extracting solution of protein；Its
In, the mass ratio of neutral proteinase and papain is 1:1.2；
(5) extracting solution of protein that step (4) obtains is filtered to be placed in ceramic micro filter film by filter cloth and is carried out
Filtering, ceramic micro filter film filter obtained filtrate and are filtered using ceramic super-filtering film, finally will be obtained by ultrafiltration membrance filter
Concentrate be spray-dried up to the high-quality tenebrio molitor protein；Wherein, the average pore size of ceramic micro filter film is
The average pore size of 200nm, crossflow velocity 2m/s, transmembrane pressure 0.15MPa, ceramic super-filtering film are 25nm, and crossflow velocity is
2.5m/s, transmembrane pressure 0.1MPa.
Embodiment 4
A kind of high-quality process for extracting bread worm protein, includes the following steps:
(1) pretreatment of yellow meal worm: to yellow meal worm carry out after cleaning removal of impurities, sterilizing, drying, crushing water content is lower than
5% yellow meal worm dry powder；
(2) degreasing: yellow meal worm dry powder quality 0.1% is accounted for by being added in pretreated yellow meal worm dry powder in step (1)
Dehydrated alcohol, then yellow meal worm dry powder is placed in the supercritical CO of tyre microwave generator2In extraction equipment, in microwave irradiation
Frequency 800MHz, temperature is 40 DEG C in extraction equipment, extracting pressure 30MPa, CO2Fluid flow extracts under conditions of being 20L/h
2h is taken, the yellow meal worm dry powder after obtaining degreasing；
(3) microwave just mentions: the yellow meal worm dry powder after step (2) degreasing is dry, and it is 0.07mol/L's that concentration is added later
NaOH solution simultaneously stirs evenly, and mixture is placed in microwave in the ratio of yellow meal worm dry powder and NaOH solution solid-liquid ratio 1:10g/mL
Microwave catalysis 90s is carried out in furnace up to tenebrio molitor protein crude extract；Wherein, power when microwave catalysis is 500W；
(4) enzyme extraction: by tenebrio molitor protein crude extract made from step (3) adjusting pH be about 7.0 after be added by
The complex enzyme of neutral proteinase and papain composition is heated to 55 DEG C later, and extracts 45min with 450W ultrasonic power,
The feed liquid after enzymatic hydrolysis is sent into enzyme deactivation device later, enzyme deactivation 5min is carried out after being warming up to 75 DEG C, obtains extracting solution of protein；Its
In, the mass ratio of neutral proteinase and papain is 1:1.2；
(5) extracting solution of protein that step (4) obtains is filtered to be placed in ceramic micro filter film by filter cloth and is carried out
Filtering, ceramic micro filter film filter obtained filtrate and are filtered using ceramic super-filtering film, finally will be obtained by ultrafiltration membrance filter
Concentrate be spray-dried up to the high-quality tenebrio molitor protein；Wherein, the average pore size of ceramic micro filter film is
The average pore size of 200nm, crossflow velocity 2m/s, transmembrane pressure 0.15MPa, ceramic super-filtering film are 25nm, and crossflow velocity is
2.5m/s, transmembrane pressure 0.1MPa.
Embodiment 5
A kind of high-quality process for extracting bread worm protein, includes the following steps:
(1) pretreatment of yellow meal worm: to yellow meal worm carry out after cleaning removal of impurities, sterilizing, drying, crushing water content is lower than
5% yellow meal worm dry powder；
(2) degreasing: yellow meal worm dry powder quality 0.1% is accounted for by being added in pretreated yellow meal worm dry powder in step (1)
Dehydrated alcohol, then yellow meal worm dry powder is placed in the supercritical CO of tyre microwave generator2In extraction equipment, in microwave irradiation
Frequency 600MHz, temperature is 40 DEG C in extraction equipment, extracting pressure 30MPa, CO2Fluid flow extracts under conditions of being 20L/h
2h is taken, the yellow meal worm dry powder after obtaining degreasing；
(3) microwave just mentions: the yellow meal worm dry powder after step (2) degreasing is dry, and it is 0.09mol/L's that concentration is added later
NaOH solution simultaneously stirs evenly, and mixture is placed in microwave in the ratio of yellow meal worm dry powder and NaOH solution solid-liquid ratio 1:12g/mL
Microwave catalysis 90s is carried out in furnace up to tenebrio molitor protein crude extract；Wherein, power when microwave catalysis is 500W；
(4) enzyme extraction: by tenebrio molitor protein crude extract made from step (3) adjusting pH be about 7.0 after be added by
The complex enzyme of neutral proteinase and papain composition is heated to 55 DEG C later, and extracts 45min with 450W ultrasonic power,
The feed liquid after enzymatic hydrolysis is sent into enzyme deactivation device later, enzyme deactivation 5min is carried out after being warming up to 75 DEG C, obtains extracting solution of protein；Its
In, the mass ratio of neutral proteinase and papain is 1:1.5；
(5) extracting solution of protein that step (4) obtains is filtered to be placed in ceramic micro filter film by filter cloth and is carried out
Filtering, ceramic micro filter film filter obtained filtrate and are filtered using ceramic super-filtering film, finally will be obtained by ultrafiltration membrance filter
Concentrate be spray-dried up to the high-quality tenebrio molitor protein；Wherein, the average pore size of ceramic micro filter film is
The average pore size of 200nm, crossflow velocity 2m/s, transmembrane pressure 0.15MPa, ceramic super-filtering film are 25nm, and crossflow velocity is
2.5m/s, transmembrane pressure 0.1MPa.
Embodiment 6
A kind of high-quality process for extracting bread worm protein, includes the following steps:
(1) pretreatment of yellow meal worm: to yellow meal worm carry out after cleaning removal of impurities, sterilizing, drying, crushing water content is lower than
5% yellow meal worm dry powder；
(2) degreasing: yellow meal worm dry powder quality 0.1% is accounted for by being added in pretreated yellow meal worm dry powder in step (1)
Dehydrated alcohol, then yellow meal worm dry powder is placed in the supercritical CO of tyre microwave generator2In extraction equipment, in microwave irradiation
Frequency 700MHz, temperature is 40 DEG C in extraction equipment, extracting pressure 32MPa, CO2Fluid flow extracts under conditions of being 22L/h
2h is taken, the yellow meal worm dry powder after obtaining degreasing；
(3) microwave just mentions: the yellow meal worm dry powder after step (2) degreasing is dry, and it is 0.08mol/L's that concentration is added later
NaOH solution simultaneously stirs evenly, and mixture is placed in microwave in the ratio of yellow meal worm dry powder and NaOH solution solid-liquid ratio 1:8g/mL
Microwave catalysis 90s is carried out in furnace up to tenebrio molitor protein crude extract；Wherein, power when microwave catalysis is 500W；
(4) enzyme extraction: by tenebrio molitor protein crude extract made from step (3) adjusting pH be about 7.0 after be added by
The complex enzyme of neutral proteinase and papain composition is heated to 55 DEG C later, and extracts 45min with 450W ultrasonic power,
The feed liquid after enzymatic hydrolysis is sent into enzyme deactivation device later, enzyme deactivation 5min is carried out after being warming up to 75 DEG C, obtains extracting solution of protein；Its
In, the mass ratio of neutral proteinase and papain is 1:1.5；
(5) extracting solution of protein that step (4) obtains is filtered to be placed in ceramic micro filter film by filter cloth and is carried out
Filtering, ceramic micro filter film filter obtained filtrate and are filtered using ceramic super-filtering film, finally will be obtained by ultrafiltration membrance filter
Concentrate be spray-dried up to the high-quality tenebrio molitor protein；Wherein, the average pore size of ceramic micro filter film is
The average pore size of 200nm, crossflow velocity 2m/s, transmembrane pressure 0.15MPa, ceramic super-filtering film are 25nm, and crossflow velocity is
2.5m/s, transmembrane pressure 0.1MPa.
Comparative example 1
A kind of high-quality process for extracting bread worm protein, includes the following steps:
(1) pretreatment of yellow meal worm: to yellow meal worm carry out after cleaning removal of impurities, sterilizing, drying, crushing water content is lower than
5% yellow meal worm dry powder；
(2) degreasing: yellow meal worm dry powder quality 0.1% is accounted for by being added in pretreated yellow meal worm dry powder in step (1)
Dehydrated alcohol, yellow meal worm dry powder is then placed in supercritical CO2In extraction equipment, temperature is 40 DEG C in extraction equipment, extraction
Pressure power is 30MPa, CO2Fluid flow extracts 2h under conditions of being 20L/h, the yellow meal worm dry powder after obtaining degreasing；
(3) microwave just mentions: the yellow meal worm dry powder after step (2) degreasing is dry, and it is 0.07mol/L's that concentration is added later
NaOH solution simultaneously stirs evenly, and mixture is placed in microwave in the ratio of yellow meal worm dry powder and NaOH solution solid-liquid ratio 1:8g/mL
Microwave catalysis 90s is carried out in furnace up to tenebrio molitor protein crude extract；Wherein, power when microwave catalysis is 400W；
(4) enzyme extraction: by tenebrio molitor protein crude extract made from step (3) adjusting pH be about 7.0 after be added by
The complex enzyme of neutral proteinase and papain composition is heated to 55 DEG C later, and extracts 45min with 450W ultrasonic power,
The feed liquid after enzymatic hydrolysis is sent into enzyme deactivation device later, enzyme deactivation 5min is carried out after being warming up to 75 DEG C, obtains extracting solution of protein；Its
In, the mass ratio of neutral proteinase and papain is 1:1.5；
(5) extracting solution of protein that step (4) obtains is filtered to be placed in ceramic micro filter film by filter cloth and is carried out
Filtering, ceramic micro filter film filter obtained filtrate and are filtered using ceramic super-filtering film, finally will be obtained by ultrafiltration membrance filter
Concentrate be spray-dried up to the high-quality tenebrio molitor protein；Wherein, the average pore size of ceramic micro filter film is
The average pore size of 200nm, crossflow velocity 2m/s, transmembrane pressure 0.15MPa, ceramic super-filtering film are 25nm, and crossflow velocity is
2.5m/s, transmembrane pressure 0.1MPa.
Comparative example 2
A kind of high-quality process for extracting bread worm protein, includes the following steps:
(1) pretreatment of yellow meal worm: to yellow meal worm carry out after cleaning removal of impurities, sterilizing, drying, crushing water content is lower than
5% yellow meal worm dry powder；
(2) degreasing: yellow meal worm dry powder quality 0.1% is accounted for by being added in pretreated yellow meal worm dry powder in step (1)
Dehydrated alcohol, then yellow meal worm dry powder is placed in the supercritical CO of tyre microwave generator2In extraction equipment, in microwave irradiation
Frequency 600MHz, temperature is 40 DEG C in extraction equipment, extracting pressure 30MPa, CO2Fluid flow extracts under conditions of being 20L/h
2h is taken, the yellow meal worm dry powder after obtaining degreasing；
(3) it just mentions: the yellow meal worm dry powder after step (2) degreasing is dry, the NaOH that concentration is 0.07mol/L is added later
Solution & stir is uniform, in the ratio of yellow meal worm dry powder and NaOH solution solid-liquid ratio 1:8g/mL extraction 1.5h up to yellow meal worm egg
White matter crude extract；
(4) enzyme extraction: by tenebrio molitor protein crude extract made from step (3) adjusting pH be about 7.0 after be added by
The complex enzyme of neutral proteinase and papain composition, is heated to enzymolysis and extraction 2h at 55 DEG C later, later will be after enzymatic hydrolysis
Feed liquid is sent into enzyme deactivation device, and enzyme deactivation 5min is carried out after being warming up to 75 DEG C, obtains extracting solution of protein；Wherein, neutral proteinase and
The mass ratio of papain is 1:1.5；
(5) extracting solution of protein that step (4) obtains is filtered to be placed in ceramic micro filter film by filter cloth and is carried out
Filtering, ceramic micro filter film filter obtained filtrate and are filtered using ceramic super-filtering film, finally will be obtained by ultrafiltration membrance filter
Concentrate be spray-dried up to the high-quality tenebrio molitor protein；Wherein, the average pore size of ceramic micro filter film is
The average pore size of 200nm, crossflow velocity 2m/s, transmembrane pressure 0.15MPa, ceramic super-filtering film are 25nm, and crossflow velocity is
2.5m/s, transmembrane pressure 0.1MPa.
Performance evaluation:
1. extracting the content of protein: being measured using triumphant formula nitriding.
2. the content of fat: being measured using soxhlet extraction.
3. ash content: being measured according to GB/T5009.4-2003 burning constant weight method.
4. moisture: being measured according to GB/T5009.4-2003 direct drying method.
To embodiment 1-6 and the just performance test of the extracted protein of comparative example 1-2, result such as the following table 1:
The content of the extracted protein of 1-6 of the embodiment of the present invention is very high it can be seen from the result of upper table 1, fat
Content is lower, and the quality of protein is good, and is superior to the extracted protein of comparative example 1-2.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (8)
1. a kind of high-quality process for extracting bread worm protein, which comprises the steps of:
(1) pretreatment of yellow meal worm: yellow meal worm dry powder is obtained after carrying out cleaning removal of impurities, sterilizing, drying, crushing to yellow meal worm；
(2) degreasing: yellow meal worm dry powder quality 0.1-0.2% is accounted for by being added in pretreated yellow meal worm dry powder in step (1)
Dehydrated alcohol, then yellow meal worm dry powder is placed in the supercritical CO of tyre microwave generator2In extraction equipment, in microwave irradiation
Frequency 500-1500MHz, temperature is about 35-45 DEG C in extraction equipment, extracting pressure 30-35MPa, CO2Fluid flow is 20-
2-3h is extracted under conditions of 24L/h, the yellow meal worm dry powder after obtaining degreasing；
(3) microwave just mentions: the yellow meal worm dry powder after step (2) degreasing is dry, and it is 0.05-0.1mol/L's that concentration is added later
NaOH solution simultaneously stirs evenly, and is placed in mixture in the ratio of yellow meal worm dry powder and NaOH solution solid-liquid ratio 1:5-15g/mL micro-
Microwave catalysis 60-240s is carried out in wave furnace up to tenebrio molitor protein crude extract；
(4) enzyme extraction: being added complex enzyme after being about 7.0 by the adjusting of tenebrio molitor protein crude extract made from step (3) pH,
It is heated to 55-65 DEG C later, and extracts 30-60min with 400-500W ultrasonic power, enzyme deactivation later obtains extracting solution of protein；
(5) extracting solution of protein that step (4) obtains is filtered to be placed in ceramic micro filter film by coarse filter and is carried out
Filtering, ceramic micro filter film filter obtained filtrate and are filtered using ceramic super-filtering film, finally will be obtained by ultrafiltration membrance filter
Concentrate be spray-dried up to the high-quality tenebrio molitor protein.
2. extraction process according to claim 1, which is characterized in that the moisture content of yellow meal worm dry powder is lower than in step (1)
5%.
3. extraction process according to claim 1, which is characterized in that the power in step (3) when microwave catalysis is 200-
800W。
4. extraction process according to claim 1, which is characterized in that complex enzyme described in step (4) is by neutral proteinase
It is formed with papain, the mass ratio of neutral proteinase and papain is 1:1-1:2.
5. extraction process according to claim 1, which is characterized in that enzyme deactivation described in step (4) is the material after digesting
Liquid is sent into enzyme deactivation device, and enzyme deactivation 4-8min is carried out after being warming up to 75-85 DEG C.
6. extraction process according to claim 1, which is characterized in that coarse filtration was carried out using filter cloth in step (5)
The average pore size of filter, ceramic micro filter film is 200-240nm, and the average pore size of crossflow velocity 2-2.5m/s, ceramic super-filtering film are
20-30nm, crossflow velocity 2.5-3.5m/s.
7. extraction process according to claim 1, which is characterized in that the inlet amount in step (5) when spray drying is
20ml/min, the revolving speed of atomizer are 1000r/min.
8. a kind of high-quality tenebrio molitor protein extracted by any one of the claim 1-7 extraction process.
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| CN113372409A (en) * | 2021-06-08 | 2021-09-10 | 山东农业工程学院 | Method for extracting tenebrio molitor protein by ultrafine grinding-ultrasonic wave-microwave coupling |
| CN113563411A (en) * | 2021-08-25 | 2021-10-29 | 青岛新农康源生物工程有限公司 | Tenebrio molitor protein extraction process |
| CN114317653A (en) * | 2021-12-23 | 2022-04-12 | 海南盛美诺生物技术有限公司 | A method for extracting elastin from bovine heart vessels |
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| CN116649456A (en) * | 2023-06-13 | 2023-08-29 | 东北农业大学 | A method for assisted degreasing of Tenebrio molitor larva protein without organic solvent residue |
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| CN110511061A (en) * | 2019-09-23 | 2019-11-29 | 红河县山地未来科技有限公司 | The method and amino acid Water soluble fertilizer of tenebrio molitor protein extraction polypeptide Amino acid |
| CN113372409A (en) * | 2021-06-08 | 2021-09-10 | 山东农业工程学院 | Method for extracting tenebrio molitor protein by ultrafine grinding-ultrasonic wave-microwave coupling |
| CN113563411A (en) * | 2021-08-25 | 2021-10-29 | 青岛新农康源生物工程有限公司 | Tenebrio molitor protein extraction process |
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| CN114317653B (en) * | 2021-12-23 | 2024-09-13 | 海南盛美诺生物技术有限公司 | A method for extracting elastin using bovine cardiac vessels |
| CN114478687A (en) * | 2022-04-01 | 2022-05-13 | 青岛浩然海洋科技有限公司 | Hippocampus protein crude extraction process |
| WO2024100577A1 (en) * | 2022-11-08 | 2024-05-16 | Universidade Católica Portuguesa | Method for fractioning of an edible insect |
| CN116649456A (en) * | 2023-06-13 | 2023-08-29 | 东北农业大学 | A method for assisted degreasing of Tenebrio molitor larva protein without organic solvent residue |
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