CN109349270A - Cell preservation solution and method for preserving cells using cell preservation solution - Google Patents
Cell preservation solution and method for preserving cells using cell preservation solution Download PDFInfo
- Publication number
- CN109349270A CN109349270A CN201811329711.8A CN201811329711A CN109349270A CN 109349270 A CN109349270 A CN 109349270A CN 201811329711 A CN201811329711 A CN 201811329711A CN 109349270 A CN109349270 A CN 109349270A
- Authority
- CN
- China
- Prior art keywords
- cell
- cells
- preservation solution
- preservation
- proclin300
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000003761 preservation solution Substances 0.000 title claims 13
- 238000004321 preservation Methods 0.000 claims abstract description 56
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 42
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 36
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 30
- 239000011780 sodium chloride Substances 0.000 claims abstract description 15
- 102000009027 Albumins Human genes 0.000 claims abstract description 14
- 108010088751 Albumins Proteins 0.000 claims abstract description 14
- 229910021555 Chromium Chloride Inorganic materials 0.000 claims abstract description 14
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 14
- QSWDMMVNRMROPK-UHFFFAOYSA-K chromium(3+) trichloride Chemical compound [Cl-].[Cl-].[Cl-].[Cr+3] QSWDMMVNRMROPK-UHFFFAOYSA-K 0.000 claims abstract description 14
- 239000008103 glucose Substances 0.000 claims abstract description 13
- 239000002504 physiological saline solution Substances 0.000 claims abstract description 10
- -1 proclin300 Chemical compound 0.000 claims abstract description 4
- 210000004027 cell Anatomy 0.000 claims description 119
- 238000003908 quality control method Methods 0.000 claims description 15
- 239000000243 solution Substances 0.000 claims description 15
- 239000006228 supernatant Substances 0.000 claims description 10
- 210000004271 bone marrow stromal cell Anatomy 0.000 claims description 5
- 239000006285 cell suspension Substances 0.000 claims description 5
- 238000000338 in vitro Methods 0.000 claims description 5
- 238000012360 testing method Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000001727 in vivo Methods 0.000 claims 1
- 238000000464 low-speed centrifugation Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 61
- 230000000694 effects Effects 0.000 abstract description 3
- 239000004615 ingredient Substances 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 238000004901 spalling Methods 0.000 abstract description 2
- 238000005119 centrifugation Methods 0.000 description 4
- 238000005138 cryopreservation Methods 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 230000001681 protective effect Effects 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 230000001225 therapeutic effect Effects 0.000 description 4
- 230000035479 physiological effects, processes and functions Effects 0.000 description 3
- 238000010257 thawing Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 102000029797 Prion Human genes 0.000 description 1
- 108091000054 Prion Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000010339 medical test Methods 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/126—Physiologically active agents, e.g. antioxidants or nutrients
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of cell-preservation liquids, including methanol, proclin300, EDTA, NaCl, acetic acid, albumin, glucose, physiological saline and chromium chloride, it is characterized by: being prepared by the component of following parts by weight: methanol: 15.00%-35.00%, proclin300:0.20%-0.35%, EDTA:0.10%-0.52%, NaCl:0.10%-0.23%, acetic acid: 53.20%-68.70%, albumin: 2.30%-25.0%, glucose: 0.10%-1.00%, physiological saline 0.5-0.9% and chromium chloride 0.1%-1%.The method for saving cell using the cell-preservation liquid, in actual use, good preservation can be carried out to the cell after acquisition, the form, activity and structure feature of original cell can be kept completely, it will not there is a phenomenon where aqtocytolysis or spallings, in addition, the ingredient of the cell-preservation liquid is simple, and deployment cost is cheap, using simple, it is easy to operate, it is suitable for promoting the use of.
Description
Technical field
The present invention relates to cell preservation technique field, specially cell-preservation liquid and use cell-preservation liquid saves cell
Method.
Background technique
Currently, normal human's living cells is with physiologic function, biological activity and complete cell shape before falling off
State has important value in biological study and medical test.If leaving human body, cell its physiology function for falling off
Energy, biological activity and cellular prion protein will also change, and therefore, after cell is detached from from human body, need by thin
Born of the same parents save liquid and maintain to form, activity and the structure of cell after disengaging human body.
But currently used cell-preservation liquid not only complicated component, but also function is more single, in addition, using cell
When preservation liquid saves cell, since the method for operation is lack of standardization, and then to cell during being saved, survival
Rate substantially reduces, for this purpose, we have proposed a kind of cell-preservation liquid and the methods for saving cell using cell-preservation liquid.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the existing defects, provides a kind of cell-preservation liquid and is protected using cell
The method that liquid storage saves cell can effectively solve in background technique cell-preservation liquid not only complicated component, but also function is more
It is single, in addition, existing when being saved using cell-preservation liquid to cell since the method for operation is lack of standardization, and then to cell
During being saved, the problem of survival rate substantially reduces.
To achieve the above object, the invention provides the following technical scheme: a kind of cell-preservation liquid, including methanol,
Proclin300, EDTA, NaCl, acetic acid, albumin, glucose, physiological saline and chromium chloride, it is characterised in that: by as follows
The component of parts by weight is prepared: methanol: 15.00%-35.00%, proclin300:0.20%-0.35%, EDTA:
0.10%-0.52%, NaCl:0.10%-0.23%, acetic acid: 53.20%-68.70%, albumin: 2.30%-25.0%, Portugal
Grape sugar: 0.10%-1.00%, physiological saline 0.5-0.9% and chromium chloride 0.1%-1%.
It as a preferred technical solution of the present invention, is prepared by the component of following parts by weight: methanol: 18.00%-
33.00%, proclin300:0.28%-0.30%, EDTA:0.22%-0.42%, NaCl:0.15%-0.20%, acetic acid:
55.38%-60.21%, albumin: 10.30%-18.91%, glucose: 0.34%-0.85%, physiological saline 0.7-0.8%
And chromium chloride 0.1%-0.5%.
It as a preferred technical solution of the present invention, is prepared by the component of following parts by weight: methanol: 25%,
Proclin300:0.29%, EDTA:0.39%, NaCl:0.18%, acetic acid: 58.45%, albumin: 15.35%, glucose:
0.56%, physiological saline 0.61% and chromium chloride 0.3%.
As a preferred technical solution of the present invention, Quality Control cell, Quality Control cell are added into above-mentioned cell-preservation liquid
For Hela cell, the concentration of Quality Control cell be added is 2 × 106A/ml, 100ul, temperature control in the range of 2-8 DEG C, protect
After depositing seven days, cell quantity is not more than 40% with quantity relative deviation when cell is just added, and the PH of above-mentioned cell-preservation liquid is answered
Control is in the range of 4.0-7.0
The method for saving cell using any one of the above cell-preservation liquid, includes the following steps: 24 before cell collection
Hour, above-mentioned Cell protective solutions are taken out from -20 DEG C of refrigerators, is put into 4 DEG C of refrigerators and melts, and saving liquid and saving concentration is 2 × 106
Cv value is no more than 10% in the cell quantity of the Hela cell liquid of a/ml batch, and cv value is no more than 15% between batch；
1. part cell is taken to carry out cell count, supernatant is removed in the centrifugation of remaining cell；106-107 is saved with every milliliter of preservation liquid
The ratio of cell is resuspended cell with preservation liquid prepared by aforementioned proportion, is placed in centrifuge tube, softly mixes well cell and preservation
Liquid；
2. cell suspension is transferred in sterile centrifuge tube, sealed with sealed membrane；
It saves, at least observes and records daily refrigerator temperature 4 times 3. centrifuge tube is transferred in 2-6 DEG C of refrigerator, guarantee refrigerator
Temperature is controlled at 2-6 DEG C；
4. the MSCs in storage life can take out in gnotobasis at any time, low-speed centrifugal abandons supernatant under the conditions of 2-6 DEG C, receives
Collect cell, is used for inside and outside；
5. cryo-conservation is no more than 14 days, when needing to detect, with 37 DEG C of quick recovery temperatures of water-bath, that is, can be used.
The method that above-mentioned cell-preservation liquid saves cell, application field are as follows: be only used for preservation, transport is derived from the thin of human body
Born of the same parents are only used for analyzed in vitro testing goal, are not used in therapeutic use.
Compared with prior art, the beneficial effects of the present invention are: the cell preservation method of the cell-preservation liquid, actually makes
Used time can carry out good preservation to the cell after acquisition, can keep the form, activity and structure of original cell completely
Feature, will not be there is a phenomenon where aqtocytolysis or spalling, in addition, the ingredient of the cell-preservation liquid is simple, deployment cost is cheap, makes
It is easy to operate with simple, it is suitable for promoting the use of.
Specific embodiment
Embodiment 1:
The present invention provides a kind of technical solution: a kind of cell-preservation liquid, including methanol, proclin300, EDTA, NaCl,
Acetic acid, albumin, glucose, physiological saline and chromium chloride, it is characterised in that: be prepared by the component of following parts by weight:
Methanol: 15.00%-35.00%, proclin300:0.20%-0.35%, EDTA:0.10%-0.52%, NaCl:0.10%-
0.23%, acetic acid: 53.20%-68.70%, albumin: 2.30%-25.0%, glucose: 0.10%-1.00%, physiology salt
Water 0.5-0.9% and chromium chloride 0.1%-1%.
Preferably, the cell-preservation liquid, store method are as follows: as a preferred technical solution of the present invention, to above-mentioned
Quality Control cell is added in cell-preservation liquid, Quality Control cell is Hela cell, and the concentration of Quality Control cell be added is 2 × 106A/
Ml, 100ul, temperature control in the range of 2-8 DEG C, and after saving seven days, cell quantity is opposite with quantity when rigid addition cell
Deviation is not more than 40%, and the PH of above-mentioned cell-preservation liquid should be controlled in the range of 4.0-7.0
Preferably, 24 hours before cell collection, above-mentioned Cell protective solutions are taken out from -20 DEG C of refrigerators, are put into 4 DEG C of refrigerators
Middle thawing saves cv value in the cell quantity batch for the Hela cell liquid that liquid preservation concentration is 2 × 106/ml and is no more than 10%, criticizes
Between cv value be no more than 15%；
Preferably, the cell-preservation liquid, store method are as follows: include the following steps: that part cell is 1. taken to carry out cytometer
Supernatant is removed in number, the centrifugation of remaining cell；The ratio that liquid saves 106-107 cell is saved with every milliliter, the guarantor prepared with aforementioned proportion
Cell is resuspended in liquid storage, is placed in centrifuge tube, softly mixes well cell and saves liquid；
2. cell suspension is transferred in sterile centrifuge tube, sealed with sealed membrane；
It saves, at least observes and records daily refrigerator temperature 4 times 3. centrifuge tube is transferred in 2-6 DEG C of refrigerator, guarantee refrigerator
Temperature is controlled at 2-6 DEG C；
4. the MSCs in storage life can take out in gnotobasis at any time, low-speed centrifugal abandons supernatant under the conditions of 2-6 DEG C, receives
Collect cell, is used for inside and outside；
5. cryo-conservation is no more than 14 days, when needing to detect, with 37 DEG C of quick recovery temperatures of water-bath, that is, can be used.
The method that above-mentioned cell-preservation liquid saves cell, application field are as follows: be only used for preservation, transport is derived from the thin of human body
Born of the same parents are only used for analyzed in vitro testing goal, are not used in therapeutic use.
Embodiment 2:
The present invention provides a kind of technical solution: a kind of cell-preservation liquid is prepared: first by the component of following parts by weight
Alcohol: 18.00%-33.00%, proclin300:0.28%-0.30%, EDTA:0.22%-0.42%, NaCl:0.15%-
0.20%, acetic acid: 55.38%-60.21%, albumin: 10.30%-18.91%, glucose: 0.34%-0.85%, physiology
Salt water 0.7-0.8% and chromium chloride 0.1%-0.5%.
Preferably, the cell-preservation liquid, store method are as follows: as a preferred technical solution of the present invention, to above-mentioned
Quality Control cell is added in cell-preservation liquid, Quality Control cell is Hela cell, and the concentration of Quality Control cell be added is 2 × 106A/
Ml, 100ul, temperature control in the range of 2-8 DEG C, and after saving seven days, cell quantity is opposite with quantity when rigid addition cell
Deviation is not more than 40%, and the PH of above-mentioned cell-preservation liquid should be controlled in the range of 4.0-7.0
Preferably, 24 hours before cell collection, above-mentioned Cell protective solutions are taken out from -20 DEG C of refrigerators, are put into 4 DEG C of refrigerators
Middle thawing saves cv value in the cell quantity batch for the Hela cell liquid that liquid preservation concentration is 2 × 106/ml and is no more than 10%, criticizes
Between cv value be no more than 15%；
Preferably, the cell-preservation liquid, store method are as follows: include the following steps: that part cell is 1. taken to carry out cytometer
Supernatant is removed in number, the centrifugation of remaining cell；The ratio that liquid saves 106-107 cell is saved with every milliliter, the guarantor prepared with aforementioned proportion
Cell is resuspended in liquid storage, is placed in centrifuge tube, softly mixes well cell and saves liquid；
2. cell suspension is transferred in sterile centrifuge tube, sealed with sealed membrane；
It saves, at least observes and records daily refrigerator temperature 4 times 3. centrifuge tube is transferred in 2-6 DEG C of refrigerator, guarantee refrigerator
Temperature is controlled at 2-6 DEG C；
4. the MSCs in storage life can take out in gnotobasis at any time, low-speed centrifugal abandons supernatant under the conditions of 2-6 DEG C, receives
Collect cell, is used for inside and outside；
5. cryo-conservation is no more than 14 days, when needing to detect, with 37 DEG C of quick recovery temperatures of water-bath, that is, can be used.
A kind of cell preservation method of above-mentioned cell-preservation liquid, application field are as follows: be only used for preservation, transport is derived from human body
Cell, be only used for analyzed in vitro testing goal, be not used in therapeutic use.
Embodiment 3:
The present invention provides a kind of technical solution: a kind of cell-preservation liquid is prepared: first by the component of following parts by weight
Alcohol: 25%, proclin300:0.29%, EDTA:0.39%, NaCl:0.18%, acetic acid: 58.45%, albumin:
15.35%, glucose: 0.56%, physiological saline 0.61% and chromium chloride 0.3%.
Preferably, the cell-preservation liquid, store method are as follows: as a preferred technical solution of the present invention, to above-mentioned
Quality Control cell is added in cell-preservation liquid, Quality Control cell is Hela cell, and the concentration of Quality Control cell be added is 2 × 106A/
Ml, 100ul, temperature control in the range of 2-8 DEG C, and after saving seven days, cell quantity is opposite with quantity when rigid addition cell
Deviation is not more than 40%, and the PH of above-mentioned cell-preservation liquid should be controlled in the range of 4.0-7.0
Preferably, 24 hours before cell collection, above-mentioned Cell protective solutions are taken out from -20 DEG C of refrigerators, are put into 4 DEG C of refrigerators
Middle thawing saves cv value in the cell quantity batch for the Hela cell liquid that liquid preservation concentration is 2 × 106/ml and is no more than 10%, criticizes
Between cv value be no more than 15%；
Preferably, the cell-preservation liquid, store method are as follows: include the following steps: that part cell is 1. taken to carry out cytometer
Supernatant is removed in number, the centrifugation of remaining cell；The ratio that liquid saves 106-107 cell is saved with every milliliter, the guarantor prepared with aforementioned proportion
Cell is resuspended in liquid storage, is placed in centrifuge tube, softly mixes well cell and saves liquid；
2. cell suspension is transferred in sterile centrifuge tube, sealed with sealed membrane；
It saves, at least observes and records daily refrigerator temperature 4 times 3. centrifuge tube is transferred in 2-6 DEG C of refrigerator, guarantee refrigerator
Temperature is controlled at 2-6 DEG C；
4. the MSCs in storage life can take out in gnotobasis at any time, low-speed centrifugal abandons supernatant under the conditions of 2-6 DEG C, receives
Collect cell, is used for inside and outside；
5. cryo-conservation is no more than 14 days, when needing to detect, with 37 DEG C of quick recovery temperatures of water-bath, that is, can be used.
A kind of cell preservation method of above-mentioned cell-preservation liquid, application field are as follows: be only used for preservation, transport is derived from human body
Cell, be only used for analyzed in vitro testing goal, be not used in therapeutic use.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811329711.8A CN109349270A (en) | 2018-11-09 | 2018-11-09 | Cell preservation solution and method for preserving cells using cell preservation solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811329711.8A CN109349270A (en) | 2018-11-09 | 2018-11-09 | Cell preservation solution and method for preserving cells using cell preservation solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109349270A true CN109349270A (en) | 2019-02-19 |
Family
ID=65344489
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811329711.8A Pending CN109349270A (en) | 2018-11-09 | 2018-11-09 | Cell preservation solution and method for preserving cells using cell preservation solution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109349270A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110305834A (en) * | 2019-05-24 | 2019-10-08 | 科索瑞生物科技(天津)有限公司 | It is a kind of for being transported at room temperature protection liquid, preparation method and the purposes of zooblast |
| CN110463686A (en) * | 2019-07-08 | 2019-11-19 | 深圳市华晨阳科技有限公司 | A kind of cell-preservation liquid that can effectively save cell for a long time |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030013074A1 (en) * | 2000-12-04 | 2003-01-16 | Kenzo Bamba | Cell-preservation liquid and method of preserving cells by using the liquid |
| CN104041484A (en) * | 2014-05-23 | 2014-09-17 | 科蒂亚(新乡)生物技术有限公司 | Cell preservation liquid |
| CN106386785A (en) * | 2016-08-30 | 2017-02-15 | 浙江博真生物科技有限公司 | Cell preservation liquid and application thereof in preservation of flow-type sample |
| CN107250346A (en) * | 2015-06-30 | 2017-10-13 | 希森美康株式会社 | Cell-preservation liquid and its utilization and the manufacture method of cell-preservation liquid |
-
2018
- 2018-11-09 CN CN201811329711.8A patent/CN109349270A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030013074A1 (en) * | 2000-12-04 | 2003-01-16 | Kenzo Bamba | Cell-preservation liquid and method of preserving cells by using the liquid |
| CN104041484A (en) * | 2014-05-23 | 2014-09-17 | 科蒂亚(新乡)生物技术有限公司 | Cell preservation liquid |
| CN107250346A (en) * | 2015-06-30 | 2017-10-13 | 希森美康株式会社 | Cell-preservation liquid and its utilization and the manufacture method of cell-preservation liquid |
| CN106386785A (en) * | 2016-08-30 | 2017-02-15 | 浙江博真生物科技有限公司 | Cell preservation liquid and application thereof in preservation of flow-type sample |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110305834A (en) * | 2019-05-24 | 2019-10-08 | 科索瑞生物科技(天津)有限公司 | It is a kind of for being transported at room temperature protection liquid, preparation method and the purposes of zooblast |
| CN110305834B (en) * | 2019-05-24 | 2021-10-29 | 科索瑞生物科技(天津)有限公司 | Protective solution for transporting animal cells at normal temperature, preparation method and application thereof |
| CN110463686A (en) * | 2019-07-08 | 2019-11-19 | 深圳市华晨阳科技有限公司 | A kind of cell-preservation liquid that can effectively save cell for a long time |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110050782B (en) | A kind of stem cell cryopreservation liquid and its preparation method and cryopreservation method | |
| AU760659B2 (en) | Improved methods for storing neural cells | |
| CN112889810B (en) | A kind of cryopreservation solution of human umbilical cord mesenchymal stem cell injection and preparation method thereof | |
| CN109349270A (en) | Cell preservation solution and method for preserving cells using cell preservation solution | |
| CN112021304A (en) | A kind of cryopreservation method and resuscitation method of umbilical cord mesenchymal stem cells | |
| CN109315386A (en) | A kind of cryopreservation solution and cryopreservation method that can be used for hematopoietic stem cells or lymphocytes | |
| CN106982822A (en) | A kind of porcine islet frozen stock solution and cryopreservation methods | |
| CN109892319A (en) | A kind of cryopreservation resuscitation method and frozen stock solution and resuscitation fluid for porcine islet | |
| CN107258766A (en) | A kind of cell freezing method and cells frozen storing liquid | |
| CN108739795A (en) | A kind of fat stem cell frozen stock solution and its cryopreservation methods | |
| JP2003267801A (en) | Preservative composition and animal cell or organ preservative containing the composition | |
| CN103798224A (en) | Sweat gland cell cryopreservation and recovery liquid and cryopreservation recovery method maintaining cell activity | |
| CN113973805A (en) | Cell cryopreservation kit and using method thereof | |
| CN113678818A (en) | Cryopreservation liquid for dental pulp or dental pulp stem cells | |
| US10278993B2 (en) | Cell-based composition and use thereof for treatment of acute stroke | |
| US20070087320A1 (en) | Medium for conservation of organs, biological tissues or living cells | |
| CN109122666A (en) | A kind of recovery instant red blood cell cryoprotector and application method | |
| CN116999429A (en) | Application of Puerarin in Preparing Medicines for Preventing or Treating Preeclampsia | |
| CN115039759B (en) | Erythrocyte preservation solution and application thereof | |
| CN115517242A (en) | A kit for cryopreservation of pig essence and its application | |
| CN110896946A (en) | A kind of hematopoietic stem cell cryopreservation solution and hematopoietic stem cell cryopreservation method | |
| CN115968863A (en) | Menstrual blood preservative fluid and cryopreservation method of menstrual blood stem cells | |
| CN108048398A (en) | A kind of stem cell cryopreserving and method for resuscitation | |
| CN108812647A (en) | Low irritability suitable for cat dog mescenchymal stem cell saves liquid and preparation method thereof | |
| CN111972397A (en) | Skin cold storage preservation solution and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190219 |
|
| RJ01 | Rejection of invention patent application after publication |