CN109288889A - A kind of antibacterial gynecological composition, gel and preparation method thereof - Google Patents
A kind of antibacterial gynecological composition, gel and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of antibacterial gynecological compositions, gel and preparation method thereof, comprise the following components in parts by weight: 2-5 parts of Chinese mugwort extracting solution, 4-10 parts of frutus cnidii, 4-10 parts of kuh-seng, 3-7 parts of safflower.Antibacterial gynecological gel of the invention, including auxiliary material and the composition, the auxiliary material includes gel-type vehicle, emulsifier, moisturizer and acidity regulator.Antibacterial gynecological composition of the invention is all used inhibiting effect to most of Gram-negative bacteria and gram-positive bacteria, ensure that drug bacteriostasis property using Chinese mugwort extracting solution as monarch drug in a prescription；Using kuh-seng, frutus cnidii as ministerial drug, gynaecological imflammation complication is assisted in the treatment of, improves drug effect, accelerates recovery from illness, both can effectively kill germ, Anti-Puritic and insecticidal, moreover it is possible to safeguard vagina acid-base balance, removing damp-heat, mass dissipating and swelling eliminating；Using safflower as adjutant, safflower is discharged the symptoms such as unsmooth and has a good effect to treatment deficiency of Yin anemia, dysmenorrhea, menstruation.
Description
Technical field
The present invention relates to gynecologic medicine technical field more particularly to a kind of antibacterial gynecological compositions, gel and its preparation side
Method.
Background technique
Gynaecological imflammation, including vaginitis, cervicitis etc. are to cause pathogen bacteria to be invaded due to outer damage or infection
And the inflammation formed.To current treatment gynecological disease to go be divided into medicine for oral administration and externally applied drug.Based on a kind of medicine of medicine for oral administration, dosage form
Including by capsule, dripping pill etc.；Then based on chemicals, dosage form is in the majority with built-in suppository, tablet for externally applied drug.Gynaecology's chemical drugs
Product excessively eat be easily destroyed lead the way flora between relationship, lead to the ill-effects such as drug resistance.The edible inconvenience of suppository, tablet, easily
Vaginal injury is caused, and suppository easily flows out, pollution clothes.Gelling agent is one of most active drug novel form in recent years, is close
The hot spot of several years local topical antibacterial gynecological gels research and development, has smooth in appearance, transparent exquisiteness；It is nonirritant, do not interfere skin
Normal function；Consistency, viscosity are suitable for being easy to be coated with use, and carrying, use, storage, transport are extremely convenient, and stability is good etc.
Advantage.The treatment of gynaecologic vaginal and cervical disease mainly has oral drugs type and topical drug at present.Since oral drugs pair is made
Slow with big and action, patient is more likely to topical drug.Topical drug is divided into Chinese patent drug, chemical drug and Chinese and Western medicament mixture again；Chemical drug
Bactericidal effect clearly, it is quick, but it is with strong points, for infecting caused by other complication and to no effect, and recurrence
Rate is high；Chinese patent drug will often cooperate other drugs simultaneously using can be only achieved effect since bactericidal effect is limited；However it is existing
Chinese patent drug effect is undesirable, and drug effect is to be improved.
Summary of the invention
It is an object of the invention to overcome the defect of the prior art, provide a kind of antibacterial gynecological composition, gel and its
Preparation method all uses inhibiting effect to part Gram-negative bacteria and gram-positive bacteria using Chinese mugwort extracting solution as monarch drug in a prescription, protects
Drug bacteriostasis property has been demonstrate,proved, it is warm-natured, there are antibacterial, antiviral, antiallergy, eliminating cold to stop pain, the functions such as warming channel for arresting bleeding；With kuh-seng,
Frutus cnidii is ministerial drug, assists in the treatment of gynaecological imflammation complication, improves drug effect, accelerates recovery from illness, both can effectively kill germ, antipruritic
Desinsection, moreover it is possible to safeguard that vagina acid-base balance, removing damp-heat, desinsection be antipruritic, mass dissipating and swelling eliminating, be suitable for bacterium, fungi, protozoon etc. and cause
Gynaecological imflammation caused by cause of disease；Using safflower as adjutant, the symptoms such as unsmooth are discharged to treatment deficiency of Yin anemia, dysmenorrhea, menstruation in safflower
It has a good effect, and also has effects that activating microcirculation and removing stasis medicinal.
The present invention is implemented as follows:
It is an object of the present invention to provide a kind of antibacterial gynecological composition, the composition includes the group of following parts by weight
Point: 2-5 parts of Chinese mugwort extracting solution, 4-10 parts of frutus cnidii, 4-10 parts of kuh-seng, 3-7 parts of safflower.
The second object of the present invention is to provide a kind of antibacterial gynecological gel, described including auxiliary material and the composition
Auxiliary material includes gel-type vehicle, emulsifier, moisturizer and acidity regulator.
Preferably, score meter by weight, described gel-type vehicle 0.3-1.5 parts；1-3 parts of emulsifier；1-5 parts of moisturizer.
Preferably, the gel-type vehicle is carbomer, xanthan gum, chitosan, sodium alginate, one in cellulose derivative
Kind is a variety of.
Preferably, the emulsifier is one or both of Tween 80, polysorbas20.
Preferably, the moisturizer is one or both of glycerine, sorbierite.
Preferably, the acidity regulator is dilute hydrochloric acid, in lactic acid, citric acid, citric acid, sodium hydroxide, triethanolamine
It is one or more, the dosage of the acidity regulator make final finished pH be 4.5-5.5.
Preferably, the auxiliary material further includes menthol and borneol, by weight score meter, described borneol 0.05-0.2 parts, thin
0.05-0.2 parts of lotus brain.
The third object of the present invention is to provide a kind of preparation method of antibacterial gynecological gel, include the following steps:
Step 1 takes the Chinese mugwort extracting solution, frutus cnidii, kuh-seng and safflower respectively in proportion, bitter by the frutus cnidii
Ginseng and safflower are soaked in water cleaning, then add Chinese mugwort extracting solution one and reinstate decocting and endure, obtain mixing Chinese medicine after finally filtering
Extracting solution；
Auxiliary material is added in step 2, the mixing Chinese medicine extract obtained by step 1, is concentrated after being mixed evenly anti-to get this
Bacterium gynecological gel.
Activity is as follows the effect of each primary raw material medicine in the present invention:
1, Chinese mugwort extracting solution: returning liver,spleen,kidney to pass through, and acrid flavour is bitter, warm-natured, and there is antibacterial, antiviral, antiallergy, cold dispelling to stop
Bitterly, the functions such as warming channel for arresting bleeding；And there is eliminating cold to stop pain, warming channel for arresting bleeding, menstruation regulating miscarriage prevention, removing dampness to relieve itching and clearing and activating the channels and collaterals and other effects.
Chinese mugwort extracting solution all uses inhibiting effect to part Gram-negative bacteria and gram-positive bacteria, to staphylococcus aureus, large intestine
Bacillus has good inhibiting effect, also there is certain inhibition to bacillus subtilis, Dipel and bacillus cereus
Effect.
2, frutus cnidii: fruit medicine, acrid flavour, hardship are warm-natured.With eliminating dampness wind-dispelling, desinsection is antipruritic, warming kidney and enhancing body and other effects.
For pruritus vulvae, scabies, eczema itch, impotence, palace cold, morbid leucorrhoea duye, arthritis with fixed pain caused by dampness lumbago；Vaginal eczema, pruritus vulvae, trichomonas is controlled outside
Vaginitis；There is inhibiting effect to drug-resistant S. aureus, Pseudomonas aeruginosa and dermatophyte；Contained xanthotoxol have compared with
Strong anti-inflammatory and analgesic activity.
3, kuh-seng: root is used as medicine, and bitter is cold in nature.There is the effect of heat-clearing and damp-drying drug, desinsection is antipruritic, diuresis.For hot dysentery, hematochezia,
Jaundice urodialysis, leukorrhea with reddish discharge, swelling of vulva pruritus vulvae, eczema, wet sore, pruitus, castor, the diseases such as trichomonas vaginitis.Kuh-seng
Decoction has obvious inhibiting effect to shigella dysenteriae, staphylococcus aureus, Escherichia coli, tubercle bacillus etc.；Trichomonas yin is controlled outside
Road is scorching.
4, safflower: safflower acrid flavour, warm-natured, converge to heart and liver channels；Gas perfume (or spice) row dissipates, and enters blood system with promoting blood circulation, dredging channels, removing blood stasis and relieving pain
Effect；Dysmenorrhea is cured mainly, menostasis, postpartum anemic fainting, stasis of blood, chest impediment and cardialgia, blood is long-pending, beats the stasis of blood swell, arthralgia, middle paralysis
Paralysis, macular eruption being dark-purple.
Compared with prior art, the invention has the advantages that and effect:
1, a kind of antibacterial gynecological composition and gel provided by the invention remove from office part using Chinese mugwort extracting solution as monarch drug in a prescription
Lan Shi negative bacterium and gram-positive bacteria all use inhibiting effect, ensure that drug bacteriostasis property, warm-natured, have antibacterial, it is antiviral,
Antiallergy, eliminating cold to stop pain, the functions such as warming channel for arresting bleeding；Using kuh-seng, frutus cnidii as ministerial drug, gynaecological imflammation complication is assisted in the treatment of, is mentioned
High-drug-effect accelerates recovery from illness, both can effectively kill germ, Anti-Puritic and insecticidal, moreover it is possible to safeguard vagina acid-base balance, removing damp-heat, kill
Worm is antipruritic, mass dissipating and swelling eliminating, suitable for gynaecological imflammation caused by the pathogenic original such as bacterium, fungi, protozoon；It is red using safflower as adjutant
It spends to treatment deficiency of Yin anemia, dysmenorrhea, menstruation are discharged the symptoms such as unsmooth and have a good effect, and also with the function of activating microcirculation and removing stasis medicinal
Effect.
2, each component is formulated in Pharmaceutical composition of the present invention is reasonable distribution: most of gynaecological imflammation is due to bacterium
Caused by group's imbalance, killing pathogenic bacteria, maintaining intravaginal colony balance is top priority, and inventor has selected biocidal property very
Good Chinese mugwort extracting solution is as main bacteriostatic agent；It is concurrent that gynaecological imflammation is accompanied by vulva and pruritus of vagina, cusalgia, redness etc.
Disease is suitable for caused by the pathogenic original such as bacterium, fungi, protozoon so inventor selects frutus cnidii and kuh-seng as adjuvant drug
Gynaecological imflammation, to heat-clearing and damp-drying drug, desinsection is antipruritic, removing toxic substances analgesic, anti-corrosion detumescence, can greatly make the life better quality；With
Safflower is adjutant, and to treatment deficiency of Yin anemia, dysmenorrhea, menstruation are discharged the symptoms such as unsmooth and have a good effect safflower, and also have
The effect of activating microcirculation and removing stasis medicinal.
3, a kind of antibacterial gynecological composition provided by the invention all has staphylococcus aureus, Escherichia coli stronger
Inhibiting effect, the minimum inhibitory concentration to staphylococcus aureus are only 0.4mg/ml, to the minimum inhibitory concentration of Escherichia coli
Only 0.8mg/ml；Four kinds of components in antibacterial gynecological composition provided by the invention cooperate, synergistic, so that its is right
Staphylococcus aureus, Escherichia coli minimum inhibitory concentration substantially reduce, bacteriostasis rate greatly increases.
Specific embodiment
Embodiment 1
1, a kind of antibacterial gynecological composition
The composition comprises the following components in parts by weight: 2 parts of Chinese mugwort extracting solution, 4 parts of frutus cnidii, and 4 parts of kuh-seng, safflower 3
Part.
2, a kind of antibacterial gynecological gel
Composition: 2 parts of Chinese mugwort extracting solution, 4 parts of frutus cnidii, 4 parts of kuh-seng, 3 parts of safflower；
Auxiliary material: 0.3 part of gel-type vehicle (xanthan gum)；1 part of emulsifier (Tween 80)；1 part of moisturizer (glycerine)；
3, the preparation method of antibacterial gynecological gel
It operates according to the following steps:
(1) after frutus cnidii, kuh-seng and safflower are dry, pulverizer crushing is respectively adopted, obtains Chinese medicine powder, powder diameter control
It is made as 60-80 mesh；
(2) frutus cnidii, kuh-seng and safflower Chinese medicine powder are taken respectively, and 5 times of inventory of water is added and is extracted；Extraction 3
Secondary, each 5h, obtains extract of Chinese traditional medicine, and the Extracting temperature of kuh-seng, frutus cnidii and safflower is 80 DEG C；
(3) the corresponding extract of Chinese traditional medicine for obtaining step (2) is added auxiliary material, is concentrated after being mixed evenly；Use lactic acid
It is adjusted to pH 4.5-5.5, stands, filter, sterilizing dispenses to obtain the final product.
Embodiment 2
1, a kind of antibacterial gynecological composition
The composition comprises the following components in parts by weight: 5 parts of Chinese mugwort extracting solution, 10 parts of frutus cnidii, 10 parts of kuh-seng, red
Spend 7 parts.
2, a kind of antibacterial gynecological gel
Composition: 5 parts of Chinese mugwort extracting solution, 10 parts of frutus cnidii, 10 parts of kuh-seng, 7 parts of safflower；
Auxiliary material: 1.5 parts of gel-type vehicle (carbomer)；3 parts of emulsifier (Tween 80)；5 parts of moisturizer (sorbierite)；
3, the preparation method of antibacterial gynecological gel
It operates according to the following steps:
(1) after frutus cnidii, kuh-seng and safflower are dry, pulverizer crushing is respectively adopted, obtains Chinese medicine powder, powder diameter control
It is made as 60-80 mesh；
(2) frutus cnidii, kuh-seng and safflower Chinese medicine powder are taken respectively, and 5 times of inventory of water is added and is extracted；Extraction 3
Secondary, each 5h, obtains extract of Chinese traditional medicine, and the Extracting temperature of kuh-seng, frutus cnidii and safflower is 80 DEG C；
(3) the corresponding extract of Chinese traditional medicine for obtaining step (2) is added auxiliary material, is concentrated after being mixed evenly；Use lactic acid
It is adjusted to pH 4.5-5.5, stands, filter, sterilizing dispenses to obtain the final product.
Embodiment 3
1, a kind of antibacterial gynecological composition
The composition comprises the following components in parts by weight: 3 parts of Chinese mugwort extracting solution, 7 parts of frutus cnidii, and 8 parts of kuh-seng, safflower 5
Part.
2, a kind of antibacterial gynecological gel
Composition: 3 parts of Chinese mugwort extracting solution, 7 parts of frutus cnidii, 8 parts of kuh-seng, 5 parts of safflower；
Auxiliary material: 1 part of gel-type vehicle (xanthan gum)；2 parts of emulsifier (Tween 80)；2 parts of moisturizer (glycerine)；
3, the preparation method of antibacterial gynecological gel
It operates according to the following steps:
(1) after frutus cnidii, kuh-seng and safflower are dry, pulverizer crushing is respectively adopted, obtains Chinese medicine powder, powder diameter control
It is made as 60-80 mesh；
(2) frutus cnidii, kuh-seng and safflower Chinese medicine powder are taken respectively, and 5 times of inventory of water is added and is extracted；Extraction 3
Secondary, each 5h, obtains extract of Chinese traditional medicine, and the Extracting temperature of kuh-seng, frutus cnidii and safflower is 80 DEG C；
(3) the corresponding extract of Chinese traditional medicine for obtaining step (2) is added auxiliary material, is concentrated after being mixed evenly；Use lactic acid
It is adjusted to pH 4.5-5.5, stands, filter, sterilizing dispenses to obtain the final product.
The formula of 1 antibacterial gynecological composition of experimental example
1, prepared by bacteriostatic agent, is divided into five groups of experimental groups: experimental group 1: Chinese mugwort extracting solution；Experimental group 2: frutus cnidii；Experimental group
3: kuh-seng；Experimental group 4: 3 parts of Chinese mugwort extracting solution, 7 parts of frutus cnidii, 8 parts of kuh-seng, 5 parts of safflower.
Precision weighs each 512mg of Chinese mugwort extracting solution, safflower, frutus cnidii, shrubby sophora extract respectively, and being dissolved in water (can be added dropwise
A small amount of DMSO dissolution solution), with 10mL volumetric flask constant volume, shake up the Chinese medicine extract for being 51.2mg/mL up to concentration, bacteriostatic agent
It was used only for the same day.4 total amount of experimental group is also the Chinese medicine extract of 51.2mg/mL.
2, antibacterial experiment in vitro strain selects
Bacillus subtilis (ATCC 6633), Escherichia coli (ATCC 35218), staphylococcus aureus (ATCC
29213)。
3, the preparation of bacteria suspension
It takes 2 generation nutrient agar slopes cultures of experimental bacteria a little, is seeded in 5ml fluid nutrient medium, suspension is made,
36 ± 1 DEG C culture 18~for 24 hours after, bacteria suspension is diluted to 0.5 Maxwell than the fluid nutrient medium with double concentration by turbidimetry
Bacterium solution is diluted to 5 × 105~5 × 106CFu/mL, this bacterium solution were used only for the same day.
4, the detection of minimum inhibitory concentration
48 sterile test tubes are taken, 4 groups of parallel tests are equally divided into, are numbered.Take first group of sterile test tube (13 × 100mm)
It 12, is arranged in a row, the Chinese medicine extract that concentration is 51.2mg/mL, No. 2 Guan Zhongjia water 1mL, mixing is added in No. 1 and No. 2 pipes
Uniformly after, draw 1mL to No. 3 pipe in, if method successively dilutes, No. 11 Guan Jiashui 1mL, at this time 1~No. 11 each pipe drug concentration according to
It is secondary be 51.2,25.6,12.8,6.4,3.2,1.6,0.8,0.4,0.2,0.1,0mg/mL,.Then it is added in 11 test tubes
The above-mentioned each 1mL of bacteria suspension prepared, shakes up.1st pipe to the 11st pipe drug concentration is respectively 25.6,12.8,6.4,3.2,
1.6, blank broth bouillon negative control the most is added in 0.8,0.4,0.2,0.1,0.05,0mg/mL, No. 12 pipes.37 DEG C of constant temperature
It is cultivated in case for 24 hours, after culture, observes culture medium, record fluid nutrient medium does not occur muddy minimum bacteriostatic agent concentration,
As minimal inhibitory concentration.
(6) frutus cnidii and kuh-seng minimum inhibitory concentration measurement result in accordance with the law
Chinese mugwort extracting solution, frutus cnidii and kuh-seng are to the minimum inhibitory concentration result of four kinds of bacteriums as shown in following table table 1:
Tetra- kinds of Chinese medicinal material extract minimum inhibitory concentrations (mg/mL) of table 1-
By selection and the collocation result of the above pharmaceutical composition it is found that antibacterial gynecological gel provided by the invention, safflower are assistant
Medicine, to treatment deficiency of Yin anemia, dysmenorrhea, menstruation are discharged the symptoms such as unsmooth and have a good effect safflower, and also have activating microcirculation and removing stasis medicinal
The effect of, remaining in 3 ingredient cooperate, it is synergistic so that it is to Escherichia coli, staphylococcus aureus, withered grass gemma
The minimum inhibitory concentration of bacillus substantially reduces, and bacteriostasis rate greatly increases.
The bacteria resistance function of 2 antibacterial gynecological gel of experimental example is tested
(1) selection of strain
The strain of selection extracorporeal bacteria inhibitor test is respectively as follows: Escherichia coli (ATCC 35218), staphylococcus aureus (ATCC
29213).Escherichia coli, staphylococcus aureus in the present invention are purchased from Nat'l Pharmaceutical & Biological Products Control Institute.
(2) carrier quantitation sterilization experiment
By " disinfection technology standard " (version in 2002) carrier quantitative germicidal test requirement, the antibacterial of the gel of above-mentioned preparation is taken
Gynecology externally used pharmaceutical combination, acts on 5min, 10min, 60min, as a result see the table below:
Table 2
Can be seen that the antibacterial gynecological gel that 1-3 of the embodiment of the present invention is provided from 2 test result of table has quick sterilization
Can, can efficient sterilizing in a short time, and can be antibacterial for a long time.
3 toxicological experiment of experimental example
Toxicological experiment is carried out to experimental animal using the antibacterial gynecological gel of embodiment 1-3, test result is as follows:
Table 3
It can be seen that from the experimental result of table 3 and experimental animal pacified using the antibacterial gynecological gel of embodiment 1- embodiment 5
Quan Xinggao, with good application prospect.
Described is only presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on should all include within protection scope of the present invention.
Claims (10)
1. a kind of antibacterial gynecological composition, which is characterized in that the composition comprises the following components in parts by weight: Chinese mugwort extracting solution
2-5 parts, 4-10 parts of frutus cnidii, 4-10 parts of kuh-seng, 3-7 parts of safflower.
2. a kind of antibacterial gynecological gel, which is characterized in that including auxiliary material and composition described in claim 1, the accessory package
Include gel-type vehicle, emulsifier, moisturizer and acidity regulator.
3. antibacterial gynecological gel as claimed in claim 2, which is characterized in that score meter by weight, the gel-type vehicle 0.3-
1.5 parts, 1-3 parts of emulsifier, 1-5 parts of moisturizer.
4. antibacterial gynecological gel as claimed in claim 2, which is characterized in that the gel-type vehicle is carbomer, xanthan gum, shell
One of glycan, sodium alginate, cellulose derivative are a variety of.
5. antibacterial gynecological gel as claimed in claim 2, which is characterized in that the emulsifier is Tween 80, in polysorbas20
It is one or two kinds of.
6. antibacterial gynecological gel as claimed in claim 2, which is characterized in that the moisturizer is glycerine, in sorbierite
It is one or two kinds of.
7. antibacterial gynecological gel as claimed in claim 2, which is characterized in that the acidity regulator is dilute hydrochloric acid, lactic acid, Chinese holly
One of rafter acid, citric acid, sodium hydroxide, triethanolamine are a variety of, and the dosage of the acidity regulator makes final finished
PH be 4.5-5.5.
8. antibacterial gynecological gel as claimed in claim 2, which is characterized in that the auxiliary material further includes menthol and borneol, is pressed
Weight fraction meter, described borneol 0.05-0.2 parts, 0.05-0.2 parts of menthol.
9. a kind of preparation method of the antibacterial gynecological gel as described in claim 2-8 is any, which is characterized in that including walking as follows
It is rapid:
Step 1 takes the Chinese mugwort extracting solution, frutus cnidii, kuh-seng and safflower respectively in proportion, by the frutus cnidii, kuh-seng and
Safflower is soaked in water cleaning, then adds Chinese mugwort extracting solution one and reinstates decocting and endure, mixing traditional Chinese medicine extraction is obtained after finally filtering
Liquid；
Auxiliary material is added in step 2, the mixing Chinese medicine extract obtained by step 1, concentration is after being mixed evenly to get antibacterial woman
Section's gel.
10. the preparation method of antibacterial gynecological gel as claimed in claim 8, which is characterized in that in the step 1, the leaching
Steeping the time is 20~40 minutes, and the decocting time is 1~2 hour.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110638985A (en) * | 2019-09-16 | 2020-01-03 | 江苏煦音生物科技有限公司 | Chinese medicinal composition gel suppository for treating gynecological inflammation and preparation method thereof |
| CN111658564A (en) * | 2020-05-26 | 2020-09-15 | 李时珍医药集团有限公司 | Gynecological vulva foam lotion and preparation method thereof |
| CN112353866A (en) * | 2020-11-21 | 2021-02-12 | 陕西远志医药生物工程有限公司 | Polypeptide nursing antibacterial gel and preparation method thereof |
| CN113940981A (en) * | 2021-11-23 | 2022-01-18 | 徐州源丰美健康科技发展有限公司 | Gynecological herbal nursing gel |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120020533A (en) * | 2010-08-30 | 2012-03-08 | 강동원 | Vaginal cleanser composition for preventing or treating a vaginitis and a kit for vaginal cleansing comprising the same |
| CN103007124A (en) * | 2012-12-28 | 2013-04-03 | 吉林大学公共卫生学院 | Chinese medicine preparation for treating gynecologic inflammation |
| CN103316266A (en) * | 2013-06-28 | 2013-09-25 | 李时珍医药集团有限公司 | Chinese herbal medicine antibacterial lotion and preparation technology thereof |
-
2018
- 2018-11-09 CN CN201811329620.4A patent/CN109288889A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120020533A (en) * | 2010-08-30 | 2012-03-08 | 강동원 | Vaginal cleanser composition for preventing or treating a vaginitis and a kit for vaginal cleansing comprising the same |
| CN103007124A (en) * | 2012-12-28 | 2013-04-03 | 吉林大学公共卫生学院 | Chinese medicine preparation for treating gynecologic inflammation |
| CN103316266A (en) * | 2013-06-28 | 2013-09-25 | 李时珍医药集团有限公司 | Chinese herbal medicine antibacterial lotion and preparation technology thereof |
Non-Patent Citations (3)
| Title |
|---|
| 本书编委会: "《药学专业知识 1 第7版 2017版》", 31 January 2017, 北京：中国医药科技出版社 * |
| 王辉武: "《中医百家药论荟萃 修订版》", 28 February 2017, 重庆：重庆出版社 * |
| 郑州博凯医药保健品有限公司: "妇洁紧致抑菌凝胶", 《企业标准信息公共服务平台》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110638985A (en) * | 2019-09-16 | 2020-01-03 | 江苏煦音生物科技有限公司 | Chinese medicinal composition gel suppository for treating gynecological inflammation and preparation method thereof |
| CN111658564A (en) * | 2020-05-26 | 2020-09-15 | 李时珍医药集团有限公司 | Gynecological vulva foam lotion and preparation method thereof |
| CN112353866A (en) * | 2020-11-21 | 2021-02-12 | 陕西远志医药生物工程有限公司 | Polypeptide nursing antibacterial gel and preparation method thereof |
| CN113940981A (en) * | 2021-11-23 | 2022-01-18 | 徐州源丰美健康科技发展有限公司 | Gynecological herbal nursing gel |
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