CN109112062A - A kind of head space Liquid sample introduction bottle aerator and aeration method - Google Patents
A kind of head space Liquid sample introduction bottle aerator and aeration method Download PDFInfo
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- CN109112062A CN109112062A CN201810927118.7A CN201810927118A CN109112062A CN 109112062 A CN109112062 A CN 109112062A CN 201810927118 A CN201810927118 A CN 201810927118A CN 109112062 A CN109112062 A CN 109112062A
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- 239000007788 liquid Substances 0.000 title claims abstract description 25
- 238000005273 aeration Methods 0.000 title claims abstract description 19
- 238000005276 aerator Methods 0.000 title claims description 22
- 238000000034 method Methods 0.000 title claims description 16
- 238000007664 blowing Methods 0.000 claims abstract description 61
- 230000001580 bacterial effect Effects 0.000 claims abstract description 25
- 230000018044 dehydration Effects 0.000 claims abstract description 17
- 238000006297 dehydration reaction Methods 0.000 claims abstract description 17
- 238000001514 detection method Methods 0.000 claims abstract description 15
- 238000010926 purge Methods 0.000 claims abstract description 15
- 239000007789 gas Substances 0.000 claims description 84
- 238000002347 injection Methods 0.000 claims description 27
- 239000007924 injection Substances 0.000 claims description 27
- 239000000243 solution Substances 0.000 claims description 22
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 14
- 229910052757 nitrogen Inorganic materials 0.000 claims description 7
- 239000003638 chemical reducing agent Substances 0.000 claims description 5
- 229920000557 Nafion® Polymers 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 230000005587 bubbling Effects 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 238000007689 inspection Methods 0.000 claims 1
- 238000005070 sampling Methods 0.000 abstract 3
- GQPLMRYTRLFLPF-UHFFFAOYSA-N Nitrous Oxide Chemical compound [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 229910002651 NO3 Inorganic materials 0.000 description 5
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000001307 helium Substances 0.000 description 2
- 229910052734 helium Inorganic materials 0.000 description 2
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 2
- 238000006396 nitration reaction Methods 0.000 description 2
- 239000001272 nitrous oxide Substances 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 1
- GQENKOUTFPKGLE-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3].[OH4+2] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3].[OH4+2] GQENKOUTFPKGLE-UHFFFAOYSA-N 0.000 description 1
- 239000005030 aluminium foil Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000004173 biogeochemical cycle Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 108010076678 nitrous oxide reductase Proteins 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 230000004043 responsiveness Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Sampling And Sample Adjustment (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
本发明涉及了一种顶空液体进样瓶曝气装置，包括供气系统，曝气系统、检测系统，供气系统和曝气系统通过吹气管路相连通，曝气系统和检测系统通过排气管路相连通，吹气管路和排气管路均有多条分支，吹气管路和排气管路的每条分支的对应位置连接有进样瓶，检测系统包括相互连接的脱水阱和气体纯度检测器，脱水阱与排气管路连接，供气系统和曝气系统之间的吹气管路和脱水阱之间连接有反吹气管路。本发明吹气管路和排气管路均有多条分支，每条分支均可连接数个进样瓶，每批可以对于多个进样瓶中的细菌溶液进行吹扫，进行批量化操作，大幅提高工作效率，同批次的样品可以同时进行曝气，加之曝气后气体纯度检测，可以有效控制批次吹扫样品本底一致化。
The invention relates to an aeration device for a headspace liquid sampling bottle, comprising an air supply system, an aeration system and a detection system. The air supply system and the aeration system are connected through an air blowing pipeline, and the aeration system and the detection system are The gas pipeline is connected, and both the blowing pipeline and the exhaust pipeline have multiple branches. The corresponding position of each branch of the blowing pipeline and the exhaust pipeline is connected with a sample bottle. The detection system includes interconnected dehydration traps and The gas purity detector, the dehydration trap is connected with the exhaust pipeline, and the blowing pipeline between the air supply system and the aeration system and the dehydration trap are connected with a backflushing gas pipeline. Both the air blowing pipeline and the exhaust pipeline of the invention have multiple branches, each branch can be connected to several sampling bottles, and each batch can purge the bacterial solution in the multiple sampling bottles for batch operation. The work efficiency is greatly improved. The samples of the same batch can be aerated at the same time. In addition, the gas purity detection after aeration can effectively control the background consistency of the batch purged samples.
Description
Technical field
The invention belongs to aeration process fields, in particular to a kind of head space Liquid sample introduction bottle aerator and control
The method of background.
Background technique
Nitrogen is a kind of important nutrient in vital movement, and nitrogen has form and valence state in more in water, mainly there is NO3 -
(+5 valence), NO2 -(+trivalent), N2O (+1 valence), N2(0 valence), NH4 +(- trivalent), it can be carried out between different valence state and form a series of
Conversion, therefore the biogeochemical cycle of nitrogen is extremely complex.
In recent years enter water body as various forms are polluted with different channels, cause water pollution to get worse, precise measurement
Nitrate nitrogen oxygen isotope in water can effectively distinguish pollutant sources (discharge of industrial wastes, farmland fertilization, human and animal excreta etc.), in turn
Scientific basis is provided targetedly to administer.
Bacterial Denitrification at One Time method is one of the common method of current nitrate nitrogen in ground water isotope analysis, and this method generates
In 2001, anti-nitration reaction is carried out to nitrate in water sample using specific bacteria, nitrogen is ultimately generated or nitrous oxide is surveyed
Nitrate is fully converted into N by examination, selection and culture, denitrifying bacterium including denitrifying bacterium2O gas, N2The extraction of O gas with
Purifying, N2O the GN 2 isotope assay, Bacterial Denitrification at One Time method can analyze nitrate nitrogen isotope in low concentration minor amount of water, fast
Reliably.
Bacterial Denitrification at One Time method is very high to nitrate responsiveness in water, but needs to cultivate specified strain, and in Spawn incubation
Environmental requirement is similarly very harsh in the process, and every sample strain activity, background control will affect test result.Since bacterium is anti-
The bacterium that nitrification process lacks nitrous oxide reductase using specific carries out anti-nitration reaction, as long as theoretically there is nitrate will be by
React into nitrous oxide, it is necessary to which effectively control background prevents atmosphere from generating contamination to sample, only prepares in same background level
The on-line analysis precision that sample out could have, it is therefore desirable to which a kind of device that bacterium solution can be made to uniform is provided.
Summary of the invention
To solve the above problems, the present invention devises a kind of head space Liquid sample introduction bottle aerator and aeration method, blow
Pipeline and gas exhaust piping have a plurality of branch, and every branch can connect several sample injection bottles, and every batch of can be for multiple sample injection bottles
In bacterial solution purged, carry out batched operation, greatly improve working efficiency, the sample with batch can carry out simultaneously
Aeration, gas purity detects be aerated in addition after, can effectively control batch purging sample copy bottom unification.
To achieve the above object, technical scheme is as follows:
A kind of head space Liquid sample introduction bottle aerator, including air supply system, aerating system, detection system, air supply system and
Aerating system is connected by blowing pipe, and aerating system is connected with detection system by gas exhaust piping, blowing pipe and row
Air pipe has an a plurality of branch, and the corresponding position of every branch of blowing pipe and gas exhaust piping is connected with sample injection bottle, detection system
System includes dehydration trap interconnected and gas purity detector, and dehydration trap is connect with gas exhaust piping, air supply system and aeration system
Blowback air pipe, the system that blowback air pipe is used to adjust aerator are connected between blowing pipe between system and dehydration trap
Pressure difference.
In the technical program, further, aerating system uses two needle modes, including blowing needle and exhaust needle, blowing needle
It is sealedly connected in blowing pipe, exhaust needle is sealedly connected on gas exhaust piping, the bottle of blowing needle and exhaust needle insertion sample injection bottle
Lid.
Further, sample injection bottle is inserted in aerating system.
Further, switch shut-off valve is installed additional on blowing needle and exhaust needle.
Further, air supply system is gas bomb, and the outlet side of gas bomb is equipped with gas pressure reducer.
Further, the gas input of every branch of blowing pipe is equipped with blowing pipe two-way valve.
Further, blowback air pipe is equipped with pressure gauge and blowback air pipe two-way valve.
Further, dehydration trap removes aqueous vapor using Nafion selectively penetrating membrane tube.
Further, gas purity detector is online flow type Nitrogen Detector.
A kind of aeration method of head space Liquid sample introduction bottle aerator, comprising the following steps:
(1) cultured bacterial solution, press seal bottle cap are pre-loaded into sample injection bottle；
(2) air supply system is connected, required pressure is adjusted to, opens at least one branch and blowback tracheae of blowing pipe
The opening quantity on road, the branch of blowing pipe is selected according to the quantity for the sample injection bottle for being packed into bacterial liquid；
(3) packaged sample bottle is inserted into aerating system, guarantees that the outlet side of blowing needle under bacterial solution liquid level, is arranged
The outlet side of air tube is on bacterial solution liquid level；
(4) sample injection bottle of sample headspace is purged using high-purity gas, high-purity gas enters sample injection bottle from blowing needle
In bacterial solution, the Atmospheric components dissolved in bacterial solution are discharged from exhaust needle, are filled by way of the exhaust of this bubblings
It splits and is changed to high-purity gas used；
(5) the exhaust end concatenation of exhaust needle is single outlet, and exit seals in dehydration trap, persistently does to the gas of discharge
It is dry, into gas purity detector, whether purged by gas purity detector feedback gas purity figure intuitive judgment sample
Completely.
In the technical program, blowing pipe and gas exhaust piping have a plurality of branch, and every branch can connect several sample introductions
Bottle, every batch of can purge the bacterial solution in multiple sample injection bottles, carry out batched operation, greatly improve work effect
Rate, the sample with batch can be aerated simultaneously, and gas purity detects be aerated in addition after, can effectively control batch purging sample
Product background unification.
In the technical program, increases detection system behind aerating system, pass through gas purity detector feedback gas
Whether purity figure intuitive judgment sample purges completely, closes air valve in time, can save with gas cost.
In the technical program, headspace sample bottle, which is inverted, is bubbled purging, can effectively blow out dissolved gas in bacterium solution, liquid falls
It sets and enters sample bottle when air can be prevented on bottle cap in plug aeration needle.
In the technical program, purging separates with exhaust needle pipe-line system and installs additional under every needle switch shut-off valve, such as blocks up
The failures such as needle need to only close corresponding failure pipeline replacement syringe needle, easy to maintain, not influence remaining normal work of system.
Detailed description of the invention
Present invention will be further explained below with reference to the attached drawings and examples.
Fig. 1 is the structural schematic diagram of head space Liquid sample introduction bottle aerator of the present invention.
Fig. 2 is the blowing pipe structural schematic diagram of head space Liquid sample introduction bottle aerator of the present invention.
Fig. 3 is the gas exhaust piping structural schematic diagram of head space Liquid sample introduction bottle aerator of the present invention.
In attached drawing:
1, air supply system 101, gas bomb 102, gas pressure reducer 2, aerating system
201, blowing needle 202, exhaust needle 3, detection system 301, dehydration trap
302, gas purity detector 4, blowing pipe 401, blowing pipe two-way valve 5, gas exhaust piping
6, sample injection bottle 601, bottle cap 7, blowback air pipe 701, pressure gauge
702, blowback air pipe two-way valve
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
As shown in Figure 1, a kind of head space Liquid sample introduction bottle aerator, it is characterised in that: including air supply system 1, aeration system
System 2, detection system 3, air supply system 1 and aerating system 2 are connected by blowing pipe 4, and aerating system 2 and detection system 3 are logical
Gas exhaust piping 5 is crossed to be connected.
Air supply system 1 is gas bomb 101, and the outlet side of gas bomb 101 is equipped with gas pressure reducer 102.
Blowing pipe 4 and gas exhaust piping 5 have a plurality of branch, and blowing pipe 4 is located at the top of gas exhaust piping 5,
As shown in Figures 2 and 3, a plurality of branch of blowing pipe 4 is in horizontally arranged, and a plurality of branch of gas exhaust piping 5 is in water
Flat raft column, and the upper and lower position of a plurality of branch of a plurality of branch and gas exhaust piping 5 of blowing pipe 4 is corresponding in turn to.The present embodiment
In, a plurality of branch of blowing pipe 4 and gas exhaust piping 5 respectively have 4 branches.
The corresponding position of every branch of blowing pipe 4 and gas exhaust piping 5 can be connected with sample injection bottle 6, every branch
Connect several sample injection bottles 6.In the present embodiment, there are 8 gas outlets in every branch of blowing pipe 4, every of gas exhaust piping 5 is divided
There are 8 air inlets in branch.Entire scavenger system is 32 (4 × 8) sample purge systems.
The front end of every branch of blowing pipe 4 is equipped with blowing pipe two-way valve 401, and blowback air pipe 7 is equipped with pressure
Power table 701 and blowback air pipe two-way valve 702.In the present embodiment, all connecting lines are all made of deactivation stainless steel tube.
Aerating system 2 uses two needle modes, including blowing needle 201 and exhaust needle 202, and blowing needle 201, which is sealedly connected on, to be blown
On air pipe 4, exhaust needle 202 is sealedly connected on gas exhaust piping 5, the bottle of blowing needle 201 and the insertion sample injection bottle 6 of exhaust needle 202
Lid 601, so that sample injection bottle 6 pours on blowing needle 201 and exhaust needle 202 in aerating system 2.
Install switch shut-off valve on blowing needle 201 and exhaust needle 202 additional.
Bottle cap 601 is aluminium foil rubber jaw bottle cap.
Detection system 3 includes dehydration trap 301 interconnected and gas purity detector 302, is dehydrated trap 301 and exhaust pipe
Road 5 connects, and is connected with blowback air pipe 7 between the blowing pipe 4 between air supply system 1 and aerating system 2 and dehydration trap 301,
Blowback air pipe 7 is used to adjust the system pressure difference of aerator.
It is dehydrated trap 301 and aqueous vapor is removed using Nafion selectively penetrating membrane tube.Gas purity detector 302 is online circulation
Formula Nitrogen Detector.
A kind of head space Liquid sample introduction bottle aeration method, comprising the following steps:
(1) cultured bacterial solution, press seal bottle cap 601 are pre-loaded into sample injection bottle 6, sample injection bottle 6 is U.S. Thermo
Scientific (match is silent to fly) National sample bottle, model C4020-25, bottle cap 601 is U.S. restek (auspicious think of Tyke)
Bottle cap, model 22359；
(2) air supply system 1 is connected, the air valve of gas bomb 101 is opened, adjusts the gas pressure reducer connecting with steel cylinder 101
102 adjust pressure to 0.4mPa, and high-purity helium is housed in gas bomb 101, open blowing pipe 4 at least one branch and
Blowback air pipe 7 needs to select the opening number of the branch of blowing pipe 4 according to the quantity for the sample injection bottle 6 for being packed into bacterial liquid
Amount；
(3) packaged sample bottle 5 is inverted insertion aerating system 2, guarantees the outlet side of blowing needle 201 in bacterial solution
Under liquid level, the outlet side of exhaust needle 202 is then on bacterial solution liquid level close to bottom of bottle；
(4) sample injection bottle 6 of sample headspace is purged using high-purity gas, high-purity gas from blowing needle 201 enter into
Bacterial solution in sample bottle 6, the Atmospheric components dissolved in bacterial solution are discharged from exhaust needle 202, pass through and this are bubbled exhaust
Mode is sufficiently displaced from as high-purity gas used；
(5) the exhaust end concatenation of exhaust needle 202 is single outlet, and exit seals in dehydration trap 301, holds to the gas of discharge
Continuous drying passes through 302 feedback gas purity figure intuitive judgment sample of gas purity detector into gas purity detector 302
Whether product purge completely, are dehydrated the model MD-050-144-S-1, the model GCT- of gas purity detector 302 of trap 301
HE-P32。
In the present embodiment, blowing pipe and gas exhaust piping have 4 branches, and every branch can connect 8 sample injection bottles, often
The bacterial solution in 32 sample injection bottles can be purged by criticizing, and carried out batched operation, greatly improved working efficiency, and same batch
Secondary sample can be aerated simultaneously, and gas purity detects be aerated in addition after, can effectively control batch purging sample copy bottom
Unification.
In the present embodiment, since purging aeration time is longer, the helium purge time was at 1 hour or more, and flow velocity is big, was exposing
Increase detection system behind gas system, whether is blown by gas purity detector feedback gas purity figure intuitive judgment sample
It sweeps completely, closes air valve in time, can save with gas cost.
In the present embodiment, headspace sample bottle, which is inverted, is bubbled purging, can effectively blow out dissolved gas in bacterium solution, liquid is inverted
Enter sample bottle when air can be prevented on bottle cap in plug aeration needle.
In the present embodiment, purging separates with exhaust needle pipe-line system and installs switch shut-off valve under every needle additional, stifled needle such as occurs
Etc. failures need to only close corresponding failure pipeline replacement syringe needle, it is easy to maintain, do not influence system remaining normal work.
Claims (10)
1. a kind of head space Liquid sample introduction bottle aerator, it is characterised in that: including air supply system (1), aerating system (2), detection
System (3), the air supply system (1) are connected with aerating system (2) by blowing pipe (4), the aerating system (2) and inspection
Examining system (3) is connected by gas exhaust piping (5), and the blowing pipe (4) and gas exhaust piping (5) have a plurality of branch, described
The corresponding position of every branch of blowing pipe (4) and gas exhaust piping (5) is connected with sample injection bottle (6), detection system (3) packet
Dehydration trap (301) interconnected and gas purity detector (302) are included, the dehydration trap (301) and gas exhaust piping (5) are even
It connects, is connected with blowback air between the blowing pipe (4) and dehydration trap (301) between the air supply system (1) and aerating system (2)
Pipeline (7).
2. aerator as described in claim 1, it is characterised in that: the aerating system (2) uses two needle modes, including blows
Air tube (201) and exhaust needle (202), the blowing needle (201) are sealedly connected on the blowing pipe (4), the exhaust needle
(202) it is sealedly connected on the gas exhaust piping (5), the blowing needle (201) and exhaust needle (202) are inserted into the sample injection bottle
(6) bottle cap (601).
3. aerator as claimed in claim 2, it is characterised in that: the sample injection bottle (6) is inserted in the aerating system (2)
In blowing needle (201) and exhaust needle (202) on.
4. aerator as claimed in claim 3, it is characterised in that: add on the blowing needle (201) and exhaust needle (202)
Dress switch shut-off valve.
5. aerator as described in claim 1, it is characterised in that: the air supply system (1) is gas bomb (101), institute
The outlet side for stating gas bomb (101) is equipped with gas pressure reducer (102).
6. aerator as described in claim 1, it is characterised in that: the gas of every branch of the blowing pipe (4) is defeated
Enter end and is equipped with blowing pipe two-way valve (401).
7. aerator as described in claim 1, it is characterised in that: the blowback air pipe (7) is equipped with pressure gauge (701)
With blowback air pipe two-way valve (702).
8. aerator as described in claim 1, it is characterised in that: the dehydration trap (301) is selectively seeped using Nafion
Permeable membrane pipe removes aqueous vapor.
9. aerator as described in claim 1, it is characterised in that: the gas purity detector (302) is online circulation
Formula Nitrogen Detector.
10. a kind of aeration method of head space Liquid sample introduction bottle aerator as claimed in claim 2, which is characterized in that including with
Lower step:
(1) cultured bacterial solution, press seal bottle cap (601) are pre-loaded into sample injection bottle (6)；
(2) air supply system (1) is connected, is adjusted to required pressure, open at least one branch and blowback tracheae of blowing pipe (4)
The opening quantity on road (7), the branch of blowing pipe (4) is selected according to the quantity for the sample injection bottle (6) for being packed into bacterial liquid；
(3) packaged sample bottle (5) is inserted into aerating system (2), guarantees the outlet side of blowing needle (201) in bacterial solution liquid
Under face, the outlet side of exhaust needle (202) is on bacterial solution liquid level；
(4) sample injection bottle (6) of sample headspace is purged using high-purity gas, high-purity gas from blowing needle (201) enter into
Bacterial solution in sample bottle (6), the Atmospheric components dissolved in bacterial solution are discharged from exhaust needle (202), pass through this bubblings row
The mode of gas is sufficiently displaced from as high-purity gas used；
(5) the exhaust end concatenation of exhaust needle (202) is single outlet, and exit seals in dehydration trap (301), holds to the gas of discharge
Continuous drying is intuitively sentenced into gas purity detector (302) by gas purity detector (302) feedback gas purity figure
Whether disconnected sample purges completely.
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| CN113804798A (en) * | 2021-10-18 | 2021-12-17 | 北京锆年领航科技有限公司 | Gas replacement purging device and method for headspace bottle |
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