CN108823252A - A kind of technique of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylitol, lignin - Google Patents
A kind of technique of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylitol, lignin Download PDFInfo
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- CN108823252A CN108823252A CN201810732898.XA CN201810732898A CN108823252A CN 108823252 A CN108823252 A CN 108823252A CN 201810732898 A CN201810732898 A CN 201810732898A CN 108823252 A CN108823252 A CN 108823252A
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- stalk
- xylitol
- lignin
- ethyl alcohol
- enzyme
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title abstract description 114
- 235000019441 ethanol Nutrition 0.000 title abstract description 61
- 229920005610 lignin Polymers 0.000 title abstract description 35
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 title abstract description 27
- 230000007071 enzymatic hydrolysis Effects 0.000 title abstract description 27
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 title abstract description 27
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 title abstract description 27
- 239000000811 xylitol Substances 0.000 title abstract description 27
- 235000010447 xylitol Nutrition 0.000 title abstract description 27
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 title abstract description 27
- 229960002675 xylitol Drugs 0.000 title abstract description 27
- 238000000034 method Methods 0.000 title abstract description 26
- 238000004519 manufacturing process Methods 0.000 title abstract description 23
- 238000000855 fermentation Methods 0.000 title abstract description 19
- 230000004151 fermentation Effects 0.000 title abstract description 19
- 230000001360 synchronised effect Effects 0.000 title abstract description 19
- 108090000790 Enzymes Proteins 0.000 abstract description 60
- 102000004190 Enzymes Human genes 0.000 abstract description 60
- 239000010902 straw Substances 0.000 abstract description 43
- 239000000835 fiber Substances 0.000 abstract description 24
- 238000000926 separation method Methods 0.000 abstract description 17
- 238000001728 nano-filtration Methods 0.000 abstract description 11
- 238000001223 reverse osmosis Methods 0.000 abstract description 11
- 239000002994 raw material Substances 0.000 abstract description 7
- 238000004821 distillation Methods 0.000 abstract description 6
- 238000005265 energy consumption Methods 0.000 abstract description 6
- 238000006243 chemical reaction Methods 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 5
- 239000012528 membrane Substances 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 229940088598 enzyme Drugs 0.000 description 59
- 239000001913 cellulose Substances 0.000 description 22
- 229920002678 cellulose Polymers 0.000 description 22
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 18
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 241000209140 Triticum Species 0.000 description 11
- 235000021307 Triticum Nutrition 0.000 description 11
- 240000008042 Zea mays Species 0.000 description 11
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 11
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 11
- 235000005822 corn Nutrition 0.000 description 11
- 229920002488 Hemicellulose Polymers 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 10
- 230000018044 dehydration Effects 0.000 description 10
- 238000006297 dehydration reaction Methods 0.000 description 10
- 239000000446 fuel Substances 0.000 description 10
- 239000007788 liquid Substances 0.000 description 10
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 9
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 9
- 108010059892 Cellulase Proteins 0.000 description 8
- 108010067035 Pancrelipase Proteins 0.000 description 8
- 229940106157 cellulase Drugs 0.000 description 8
- 229940059442 hemicellulase Drugs 0.000 description 8
- 108010002430 hemicellulase Proteins 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 241000209094 Oryza Species 0.000 description 7
- 235000007164 Oryza sativa Nutrition 0.000 description 7
- AEMOLEFTQBMNLQ-BKBMJHBISA-N alpha-D-galacturonic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-BKBMJHBISA-N 0.000 description 7
- 235000013339 cereals Nutrition 0.000 description 7
- 230000007062 hydrolysis Effects 0.000 description 7
- 238000006460 hydrolysis reaction Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 235000009566 rice Nutrition 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 241000209219 Hordeum Species 0.000 description 6
- 235000007340 Hordeum vulgare Nutrition 0.000 description 6
- 240000006394 Sorghum bicolor Species 0.000 description 6
- 235000015505 Sorghum bicolor subsp. bicolor Nutrition 0.000 description 6
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 6
- 238000001914 filtration Methods 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 238000003860 storage Methods 0.000 description 6
- 239000010907 stover Substances 0.000 description 6
- 230000015556 catabolic process Effects 0.000 description 5
- 238000001816 cooling Methods 0.000 description 5
- 238000002425 crystallisation Methods 0.000 description 5
- 230000008025 crystallization Effects 0.000 description 5
- 238000006731 degradation reaction Methods 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 239000012535 impurity Substances 0.000 description 5
- 238000004898 kneading Methods 0.000 description 5
- 238000001471 micro-filtration Methods 0.000 description 5
- 239000002002 slurry Substances 0.000 description 5
- 238000000108 ultra-filtration Methods 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 239000006174 pH buffer Substances 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 229920001277 pectin Polymers 0.000 description 3
- 239000001814 pectin Substances 0.000 description 3
- 235000010987 pectin Nutrition 0.000 description 3
- 208000009146 rhinoscleroma Diseases 0.000 description 3
- 239000002023 wood Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009996 mechanical pre-treatment Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07G—COMPOUNDS OF UNKNOWN CONSTITUTION
- C07G1/00—Lignin; Lignin derivatives
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
- C08H6/00—Macromolecular compounds derived from lignin, e.g. tannins, humic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical & Material Sciences (AREA)
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- Engineering & Computer Science (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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Abstract
The invention discloses the techniques of a kind of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylitol, lignin, include the following steps：（1）Pretreatment of raw material；（2）Enzymatic hydrolysis；（3）Separation；（4）Distillation, fermentation, nanofiltration；（5）Reverse osmosis purifying.Stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol provided by the invention, xylitol, lignin technique bioenzymatic conversion technology and membrane separation technique are organically combined, plant straw fibers matter enzyme used can destroy the labyrinth of stalk, improve enzymolysis efficiency, membrane separation process is simple, low energy consumption, it can be realized synchronous production multiple product, maximally utilize agricultural straw resource, generate good economic benefit.
Description
Technical field
The present invention relates to technical field of biochemical industry, and in particular to a kind of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylose
The technique of alcohol, lignin.
Background technique
With the rapid development of economic society, resource consumption is getting faster, and resource scarcity, which has become, restricts global evolution
Serious problem.866,600,000,000 tons of plant organic matters are approximately formed on the earth every year, are that distributed in nature is most wide, content is most, price
Renewable resource that is cheap and being underutilized.The energy is converted by plant fiber waste by biotechnology, is changed
Chemical product, to solving, energy crisis is of far-reaching significance.
In recent years, polysaccharide is extracted and by polysaccharide conversion at the technology of alcohol using microorganism enzymatic hydrolysis stalk, can save grain
Food can make full use of renewable resource again.However microorganism enzymatic hydrolysis stalk products obtained therefrom low yield, water consume are high, energy consumption height, generate
A large amount of residues both increase production cost in this way, also pollute the environment.Therefore, exploitation can improve the warp of stalk enzymatic hydrolysis
Ji benefit, but the production technology that stalk resource can be promoted to comprehensively utilize is of great significance to low-carbon economy development.
Summary of the invention
In view of the deficienciess of the prior art, the present invention provides a kind of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylose, wood
The technique of quality.
The purpose of the present invention is achieved through the following technical solutions：
The technique of a kind of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylitol, lignin, includes the following steps：
(1) pretreatment of raw material：Straw is kneaded into 4-8cm filiform with wire kneading machine, stoking is without scleroma；
(2) it digests：By -06 series mixing of pretreated stalk and the cellulosic enzyme of corresponding straw type, water, pH is added
Buffer, then with the double-deck material bag packing is sealed, indoor storage, hydrolysis temperature is 2-30 DEG C, and enzymolysis time is 7-150 days, pH
Value≤7；
(3) it separates：Zymolyte is directly loadable into vacuum pulp washing machine, by squeezing, filtering, by liquid from cellulose slurry
It separates, after liquid is sent into micro-filtration extraction lignin, continues to produce second after being re-fed into ultrafiltration apparatus recycled fiber matter enzyme
Pure and mild xylitol；
(4) it distills, ferment, nanofiltration：It is passed through vapor in ethanol solution, controlled at 78.5-80 DEG C, ethyl alcohol is steamed
Gas is sent out into, steam carries out dehydration purification through cooling reverse osmosis unit of being sent into；Product, that is, liquid glucose passes through under aerobic conditions after distillation
It is sent into nanofiltration device after everfermentation and carries out dehydration separation, then purified, crystallization, separation, drying process, produces finished product xylose
Alcohol；
(5) reverse osmosis purifying：By the water removal in ethanol solution, motive force is pressure difference, pressure be 1-10MPa to get
Alcohol fuel, alcohol fuel contain the impurity such as a small amount of methanol, can further rectifying removal.
Further, straw of the present invention is corn stover, broomcorn straw, wheat stalk, Barley straw, rice
One or more of stalk, grain straw.
Further, -06 series of plant straw fibers matter enzyme of the present invention is multi-functional complex enzyme, by cellulase,
The composition such as hemicellulase, lignoenzyme, pectic substance enzyme, protease, zymase, these enzyme components, which can cooperate with, destroys stalk group
Structure is knitted, cellulose, hemicellulose, lignin degradation ethyl alcohol, xylitol, polymeric lignin are made.Plant straw fibers matter enzyme
It is divided into 3 classes according to straw type, corn stalk fiber matter enzyme -06 is suitable for corn and broomcorn straw；Wheat straw fibre matter enzyme -06
Suitable for wheat, barley；Straw fibers matter enzyme -06 is suitable for rice, grain straw.
Under the action of plant straw fibers matter enzyme, stalk institutional framework decomposable process is：In the common of enzyme and hydrone
Under effect, first hydrone gradually penetrate into stalk tissue make stalk tissue generate expansion, make knot of tissue generate variation, tissue by
Close to become thin, water soluble pectin dissolution, this just enters substrate surface for enzyme and provides channel；Secondly as enzyme enters substrate surface,
The function that enzyme component begins to play hydrolysis substrate makes pectin object be hydrolyzed into solable matter, makes under the action of pectic substance enzyme
Histocyte separation, until histocyte thoroughly becomes discrete state；In the association of cellulase, hemicellulase, lignoenzyme
Under same-action, cellulose, hemicellulose, the advanced labyrinth formed between lignin are destroyed, cellulose, hemicellulose
Element, lignin just become discrete state；Finally, in cellulose components, hemicellulase component, the effect of lignoenzyme component
Under, corresponding substrate is further hydrolyzed to glucose, xylose, lignin, and glucose is converted into second under the action of zymase
Alcohol, xylose further microbe conversion under aerobic conditions is xylitol.
Stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol provided by the invention, xylitol, lignin technique by bioenzymatic conversion
Technology and membrane separation technique organically combine, and maximally utilize agricultural straw resource.In the feelings for not changing technique, equipment
Under condition, using corn stover, Wheat Straw, rice straw as raw material, research and development adaptable fibre therewith the characteristics of for each stalk
Matter enzyme is tieed up, stalk active principle limitization is utilized, synchronous production multiple product greatly reduces process costs, overcomes existing
Have stalk enzymolysis time is long, difficulty is big, product is single, water consumption, energy consumption problem.
The membrane separation technique that the present invention uses has the function of separation, concentration, purifying and purification, energy-efficient, environmentally friendly, filtering
Process is simple, easily controllable；It carries out at normal temperature, only electric energy is needed to push, energy consumption is about the 1/3-1/8 being concentrated by evaporation；It is allusion quotation
The physical separating process of type, does not have to chemical reagent, and product is not contaminated.
The present invention has the advantages that compared with prior art：
(1) stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol provided by the invention, xylitol, the technique of lignin and existing stalk
Enzymolysis process is compared, and using cellulose material enzyme corresponding with straw type, compared with marketed cellulose enzyme, enzymatic hydrolysis condition is mild,
Only need room temperature that can carry out, enzymolysis efficiency is high, can destroy the labyrinth of stalk, stalk is without complicated pretreatment, packing storage
Enzymolysis process can be carried out by depositing, and simplified operation, saved equipment cost, overcome the enzymatic hydrolysis and fermentation period it is long caused by equipment at
This big and energy consumption height, the limited defect of scale；
(2) technique of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol provided by the invention, xylitol, lignin is using film point
From technology, simple process is easy to operate, and the degree of automation is higher, and low energy consumption, pollution-free, can be realized returning for cellulose material enzyme
It receives, greatly reduces enzyme cost；
(3) technique of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol provided by the invention, xylitol, lignin, can be large quantities of
Continuous production is measured, technological flexibility is high, and cellulose, hemicellulose, the lignin in stalk are completely converted into production product, ethyl alcohol
High with xylitol conversion rate, hemicellulose utilization rate is high, good in economic efficiency, to comprehensive utilization of resources and it is energy-efficient have it is important
Meaning.
Detailed description of the invention
Fig. 1 is the flow chart of the technique of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylitol, lignin.
Specific embodiment
Further to illustrate technological means and its effect adopted by the present invention, below in conjunction with drawings and the specific embodiments
It is described in detail.
Embodiment 1
The technique (shown in Fig. 1) of a kind of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylitol, lignin, including following step
Suddenly：
(1) pretreatment of raw material：Corn stover, broomcorn straw are kneaded into 4-8cm filiform with wire kneading machine, stoking is without scleroma；
(2) it digests：1kg corn stalk fiber matter enzyme-is added by the pretreated corn stover of every 200kg, broomcorn straw
01 is uniformly mixed, and is then respectively adding water, pH buffer, is then packaged with the double-deck material bag of sealing, indoor storage, and hydrolysis temperature is
2-30 DEG C, enzymolysis time is 7-150 days, pH value≤7；
(3) it separates：After the completion of enzymatic hydrolysis, zymolyte is directly loadable into vacuum pulp washing machine, by squeezing, filtering, by liquid
It is separated from cellulose slurry, after liquid is sent into micro-filtration extraction lignin, is re-fed into ultrafiltration apparatus recycled fiber matter enzyme
After continue to produce ethyl alcohol and xylitol；
(4) it distills, ferment, nanofiltration：It is passed through vapor in ethanol solution, controlled at 78.5-80 DEG C, ethyl alcohol is steamed
Gas is sent out into, steam carries out dehydration purification through cooling reverse osmosis unit of being sent into；Product, that is, liquid glucose passes through under aerobic conditions after distillation
It is sent into nanofiltration device after everfermentation and carries out dehydration separation, then purified, crystallization, separation, drying process, produces finished product xylose
Alcohol；
(5) reverse osmosis purifying：By the water removal in ethanol solution, motive force is pressure difference, pressure be 1-10MPa to get
Alcohol fuel, alcohol fuel contain the impurity such as a small amount of methanol, can further rectifying removal.
Further, corn stalk fiber matter enzyme -06 of the present invention is multi-functional complex enzyme, is 5 by quality proportioning:5:
1:1:The composition such as 5 cellulase, hemicellulase, lignoenzyme, pectic substance enzyme, zymase, these enzyme components can cooperate with
Corn stover, the institutional framework of broomcorn straw are destroyed, makes cellulose, hemicellulose, lignin degradation ethyl alcohol, xylitol, gather
Close lignin.
Embodiment 2
The technique (shown in Fig. 1) of a kind of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylitol, lignin, including following step
Suddenly：
(1) pretreatment of raw material：Wheat stalk, Barley straw are kneaded into 4-8cm filiform with wire kneading machine respectively, stoking is without hard
Knot；
(2) it digests：1.5kg wheat straw fibre matter enzyme-is added by the pretreated wheat stalk of every 200kg, Barley straw
01 is uniformly mixed, and is then respectively adding water, pH buffer, is then packaged with the double-deck material bag of sealing, indoor storage, and hydrolysis temperature is
2-30 DEG C, enzymolysis time is 7-150 days, pH value≤7；
(3) it separates：After the completion of enzymatic hydrolysis, zymolyte is directly loadable into vacuum pulp washing machine, by squeezing, filtering, by liquid
It is separated from cellulose slurry, after liquid is sent into micro-filtration extraction lignin, is re-fed into ultrafiltration apparatus recycled fiber matter enzyme
After continue to produce ethyl alcohol and xylitol；
(4) it distills, ferment, nanofiltration：It is passed through vapor in ethanol solution, controlled at 78.5-80 DEG C, ethyl alcohol is steamed
Gas is sent out into, steam carries out dehydration purification through cooling reverse osmosis unit of being sent into；Product, that is, liquid glucose passes through under aerobic conditions after distillation
It is sent into nanofiltration device after everfermentation and carries out dehydration separation, then purified, crystallization, separation, drying process, produces finished product xylose
Alcohol；
(5) reverse osmosis purifying：By the water removal in ethanol solution, motive force is pressure difference, pressure be 1-10MPa to get
Alcohol fuel, alcohol fuel contain the impurity such as a small amount of methanol, can further rectifying removal.
Further, wheat straw fibre matter enzyme -06 of the present invention is multi-functional complex enzyme, is 6 by quality proportioning:4:1:
1:6 cellulase, hemicellulase, lignoenzyme, pectic substance enzyme, zymase composition, these enzyme components can cooperate with destruction
The institutional framework of Barley straw, wheat stalk makes cellulose, hemicellulose, lignin degradation ethyl alcohol, xylitol, polymerization wood
Quality.
Embodiment 3
The technique (shown in Fig. 1) of a kind of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylitol, lignin, including following step
Suddenly：
(1) pretreatment of raw material：Rice straw, grain straw are kneaded into 4-8cm filiform with wire kneading machine respectively, stoking is without hard
Knot；
(2) it digests：1kg paddy stalk fibre matter enzyme-is added by the pretreated rice straw of every 200kg, grain straw
01 is uniformly mixed, and is then respectively adding water, pH buffer, is then packaged with the double-deck material bag of sealing, indoor storage, and hydrolysis temperature is
2-30 DEG C, enzymolysis time is 7-150 days, pH value≤7；
(3) it separates：After the completion of enzymatic hydrolysis, zymolyte is directly loadable into vacuum pulp washing machine, by squeezing, filtering, by liquid
It is separated from cellulose slurry, after liquid is sent into micro-filtration extraction lignin, is re-fed into ultrafiltration apparatus recycled fiber matter enzyme
After continue to produce ethyl alcohol and xylitol；
(4) it distills, ferment, nanofiltration：It is passed through vapor in ethanol solution, controlled at 78.5-80 DEG C, ethyl alcohol is steamed
Gas is sent out into, steam carries out dehydration purification through cooling reverse osmosis unit of being sent into；Product, that is, liquid glucose passes through under aerobic conditions after distillation
It is sent into nanofiltration device after everfermentation and carries out dehydration separation, then purified, crystallization, separation, drying process, produces finished product xylose
Alcohol；
(5) reverse osmosis purifying：By the water removal in ethanol solution, motive force is pressure difference, pressure be 1-10MPa to get
Alcohol fuel, alcohol fuel contain the impurity such as a small amount of methanol, can further rectifying removal.
Further, paddy stalk fibre matter enzyme -06 of the present invention is multi-functional complex enzyme, is 6 by quality proportioning:5:
1:1:6 cellulase, hemicellulase, lignoenzyme, pectic substance enzyme, zymase composition, these enzyme components can cooperate with brokenly
The institutional framework of bad rice straw, grain straw makes cellulose, hemicellulose, lignin degradation ethyl alcohol, xylitol, polymerization
Lignin.
Embodiment 4
The technique (shown in Fig. 1) of a kind of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylitol, lignin, including following step
Suddenly：
(1) pretreatment of raw material：By corn stover, broomcorn straw, wheat stalk, Barley straw, rice straw, grain straw
It is kneaded into 4-8cm filiform with wire kneading machine respectively, stoking is without scleroma；
(2) it digests：The cellulosic enzyme -01 of the corresponding straw type of 1.5kg is added by the pretreated straw of every 200kg
Series is uniformly mixed, and is then respectively adding water, pH buffer, is then packaged with the double-deck material bag of sealing, indoor storage, hydrolysis temperature
It is 2-30 DEG C, enzymolysis time is 7-150 days, pH value≤7；
(3) it separates：After the completion of enzymatic hydrolysis, zymolyte is directly loadable into vacuum pulp washing machine, by squeezing, filtering, by liquid
It is separated from cellulose slurry, after liquid is sent into micro-filtration extraction lignin, is re-fed into ultrafiltration apparatus recycled fiber matter enzyme
After continue to produce ethyl alcohol and xylitol；
(4) it distills, ferment, nanofiltration：It is passed through vapor in ethanol solution, controlled at 78.5-80 DEG C, ethyl alcohol is steamed
Gas is sent out into, steam carries out dehydration purification through cooling reverse osmosis unit of being sent into；Product, that is, liquid glucose passes through under aerobic conditions after distillation
It is sent into nanofiltration device after everfermentation and carries out dehydration separation, then purified, crystallization, separation, drying process, produces finished product xylose
Alcohol；
(6) reverse osmosis purifying：By the water removal in ethanol solution, motive force is pressure difference, pressure be 1-10MPa to get
Alcohol fuel, alcohol fuel contain the impurity such as a small amount of methanol, can further rectifying removal.
Further, -06 series of plant fiber matter enzyme of the present invention is multi-functional complex enzyme, corn stalk fiber used
Matter enzyme -06 is 5 by quality proportioning:5:1:1:5 cellulase, hemicellulase, lignoenzyme, pectic substance enzyme, zymase group
At；Wheat straw fibre matter enzyme -06 is 6 by quality proportioning:4:1:1:6 cellulase, hemicellulase, lignoenzyme, pectin
Matter enzyme, zymase composition；Paddy stalk fibre matter enzyme -06 is 6 by quality proportioning:5:1:1:6 cellulase, hemicellulose
Enzyme, lignoenzyme, pectic substance enzyme, zymase composition；These enzyme components can cooperate with the institutional framework for destroying corresponding stalk, make each
Class stalk institute containing cellulose, hemicellulose, lignin degradation are ethyl alcohol, xylitol, polymeric lignin.
Comparative example 1
In addition to enzyme used is marketed cellulose enzyme, remaining is the same as embodiment 1.
Comparative example 2
In addition to enzyme used is marketed cellulose enzyme, remaining is the same as embodiment 2.
Comparative example 3
In addition to enzyme used is marketed cellulose enzyme, remaining is the same as embodiment 3.
Comparative example 4
In addition to enzyme used is marketed cellulose enzyme, remaining is the same as embodiment 4.
Interpretation of result
1~4 products obtained therefrom yield of Examples 1 to 4 and comparative example (every 200kg stalk) is shown in Table 1.
1 unit of table：kg
| Product type | Ethyl alcohol | Xylitol | Lignin |
| Embodiment 1 | 31.83 | 49.02 | 8.11 |
| Embodiment 2 | 41.28 | 34.5 | 15.90 |
| Embodiment 3 | 37.69 | 43.09 | 10.55 |
| Embodiment 4 | 36.50 | 43.12 | 11.54 |
| Comparative example 1 | 3.50 | 1.31 | - |
| Comparative example 2 | 4.11 | 1.23 | - |
| Comparative example 3 | 3.62 | 1.22 | - |
| Comparative example 4 | 3.68 | 1.27 | - |
As can be seen from the above data, stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, the wood of the offer of the embodiment of the present invention 1~4
Sugar alcohol, lignin the yield of technique products obtained therefrom be significantly larger than comparative example 1~4, show that straw provided by the invention is fine
It ties up the serial complex enzyme of matter enzyme -06 to be adapted with corresponding straw type and production product category, can targetedly destroy stalk
Composed structure, improve enzymolysis efficiency, stalk only needs simple and mechanical pretreatment, and hydrolysis result is good, and stalk effective component is filled
Divide and utilize, product yield is high, good in economic efficiency.And marketed cellulose enzyme cannot destroy stalk institutional framework, product yield is extremely low.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, ability
Other modifications or equivalent replacement that domain those of ordinary skill makes technical solution of the present invention, without departing from skill of the present invention
The spirit and scope of art scheme, are intended to be within the scope of the claims of the invention.
Claims (5)
1. the technique of a kind of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylitol, lignin, which is characterized in that including following step
Suddenly：
（1）Pretreatment of raw material：Straw is kneaded into 4-8 cm filiform with wire kneading machine, stoking is without scleroma；
（2）Enzymatic hydrolysis：By -06 series mixing of pretreated stalk and the cellulosic enzyme of corresponding straw type, water, pH buffering is added
Liquid, then with the double-deck material bag packing is sealed, indoor storage is digested；
（3）Separation：Zymolyte is directly loadable into vacuum pulp washing machine, by squeezing, filtering, liquid is separated from cellulose slurry
Out, liquid is sent into after micro-filtration extracts lignin, be re-fed into after ultrafiltration apparatus recycled fiber matter enzyme continue to produce ethyl alcohol and
Xylitol；
（4）Distillation, fermentation, nanofiltration：Vapor is passed through in ethanol solution, by ethanol evaporation at gas, steam is sent into through cooling
Reverse osmosis unit carries out dehydration purification；Product, that is, liquid glucose is sent into nanofiltration device after everfermentation under aerobic conditions and is carried out after distillation
Dehydration separation, then purified, crystallization, separation, drying process, produce finished product xylitol；
（5）Reverse osmosis purifying：By the water removal in ethanol solution, motive force is pressure difference, and pressure is 1-10 MPa to get fuel
Ethyl alcohol, alcohol fuel contain the impurity such as a small amount of methanol, can further rectifying removal.
2. the technique of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol according to claim 1, xylitol, lignin, feature
It is, the straw is corn stover, in broomcorn straw, wheat stalk, Barley straw, rice straw, grain straw
It is one or more of.
3. the technique of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol according to claim 1, xylitol, lignin, feature
It is, -06 series of plant straw fibers matter enzyme is multi-functional complex enzyme, and enzyme includes cellulase, hemicellulase, wood
Quality enzyme, pectic substance enzyme, protease, zymase.
4. the technique of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol according to claim 1, xylitol, lignin, feature
It is, step（2）Middle hydrolysis temperature is 2-30 DEG C, and enzymolysis time is 7-150 days, pH value≤7.
5. the technique of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol according to claim 1, xylitol, lignin, feature
It is, step（4）Middle ethanol evaporation process control temp is 78.5-80 DEG C.
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