CN108753847A - A kind of technique of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylo-oligosaccharide, lignin, cellulose fiber slurry - Google Patents
A kind of technique of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylo-oligosaccharide, lignin, cellulose fiber slurry Download PDFInfo
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- CN108753847A CN108753847A CN201810731301.XA CN201810731301A CN108753847A CN 108753847 A CN108753847 A CN 108753847A CN 201810731301 A CN201810731301 A CN 201810731301A CN 108753847 A CN108753847 A CN 108753847A
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- Prior art keywords
- stalk
- oligosaccharide
- xylo
- lignin
- ethyl alcohol
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 143
- 235000019441 ethanol Nutrition 0.000 title claims abstract description 68
- 229920005610 lignin Polymers 0.000 title claims abstract description 43
- 239000002002 slurry Substances 0.000 title claims abstract description 37
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 title claims abstract description 36
- 229920003043 Cellulose fiber Polymers 0.000 title claims abstract description 32
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 29
- 230000007071 enzymatic hydrolysis Effects 0.000 title claims abstract description 24
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 title claims abstract description 24
- 230000001360 synchronised effect Effects 0.000 title claims abstract description 24
- 238000000855 fermentation Methods 0.000 title claims abstract description 23
- 230000004151 fermentation Effects 0.000 title claims abstract description 23
- 108090000790 Enzymes Proteins 0.000 claims abstract description 69
- 102000004190 Enzymes Human genes 0.000 claims abstract description 69
- 239000010902 straw Substances 0.000 claims abstract description 58
- 239000000835 fiber Substances 0.000 claims abstract description 37
- 238000004821 distillation Methods 0.000 claims abstract description 13
- 238000001728 nano-filtration Methods 0.000 claims abstract description 13
- 238000001223 reverse osmosis Methods 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 238000000926 separation method Methods 0.000 claims abstract description 9
- 229940088598 enzyme Drugs 0.000 claims description 68
- 229920002678 cellulose Polymers 0.000 claims description 33
- 239000001913 cellulose Substances 0.000 claims description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 13
- 241000209140 Triticum Species 0.000 claims description 12
- 235000021307 Triticum Nutrition 0.000 claims description 12
- 240000008042 Zea mays Species 0.000 claims description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 12
- 239000000446 fuel Substances 0.000 claims description 12
- 108010067035 Pancrelipase Proteins 0.000 claims description 9
- 108010002430 hemicellulase Proteins 0.000 claims description 9
- 229940059442 hemicellulase Drugs 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 9
- 241000196324 Embryophyta Species 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 235000007164 Oryza sativa Nutrition 0.000 claims description 8
- AEMOLEFTQBMNLQ-BKBMJHBISA-N alpha-D-galacturonic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-BKBMJHBISA-N 0.000 claims description 8
- 235000013339 cereals Nutrition 0.000 claims description 8
- 238000001704 evaporation Methods 0.000 claims description 8
- 230000008020 evaporation Effects 0.000 claims description 8
- 235000009566 rice Nutrition 0.000 claims description 8
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 7
- 240000006394 Sorghum bicolor Species 0.000 claims description 7
- 235000015505 Sorghum bicolor subsp. bicolor Nutrition 0.000 claims description 7
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 7
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 7
- 230000018044 dehydration Effects 0.000 claims description 7
- 238000006297 dehydration reaction Methods 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 230000007062 hydrolysis Effects 0.000 claims description 7
- 238000006460 hydrolysis reaction Methods 0.000 claims description 7
- 235000009973 maize Nutrition 0.000 claims description 7
- 238000003860 storage Methods 0.000 claims description 7
- 239000007844 bleaching agent Substances 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 6
- 239000012535 impurity Substances 0.000 claims description 6
- 238000004898 kneading Methods 0.000 claims description 6
- 238000001471 micro-filtration Methods 0.000 claims description 6
- 238000000746 purification Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- 238000000108 ultra-filtration Methods 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- 208000009146 rhinoscleroma Diseases 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 2
- 240000005979 Hordeum vulgare Species 0.000 claims 1
- 240000007594 Oryza sativa Species 0.000 claims 1
- 108091005804 Peptidases Proteins 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 230000003139 buffering effect Effects 0.000 claims 1
- 238000004886 process control Methods 0.000 claims 1
- 238000005265 energy consumption Methods 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 5
- 239000012528 membrane Substances 0.000 abstract description 5
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 10
- 229920002488 Hemicellulose Polymers 0.000 description 9
- 108010059892 Cellulase Proteins 0.000 description 8
- 229940106157 cellulase Drugs 0.000 description 8
- 241000209094 Oryza Species 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 241000209219 Hordeum Species 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 5
- 239000007853 buffer solution Substances 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 5
- 235000005822 corn Nutrition 0.000 description 5
- 238000006731 degradation reaction Methods 0.000 description 5
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229960003487 xylose Drugs 0.000 description 3
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 150000004804 polysaccharides Chemical class 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000009996 mechanical pre-treatment Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 229920001221 xylan Polymers 0.000 description 1
- 150000004823 xylans Chemical class 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
- C08H6/00—Macromolecular compounds derived from lignin, e.g. tannins, humic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Life Sciences & Earth Sciences (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Materials Engineering (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses the techniques of a kind of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylo-oligosaccharide, lignin, cellulose fiber slurry, include the following steps：（1）Pretreatment of raw material；（2）Enzymolysis；（3）Separation；（4）Distillation, nanofiltration；（5）Reverse osmosis purifying.Stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol provided by the invention, xylo-oligosaccharide, lignin, cellulose fiber slurry technique bioenzymatic conversion technology and membrane separation technique are organically combined, plant straw fibers matter enzyme used can destroy the labyrinth of stalk, improve enzymolysis efficiency, membrane separation process is simple, low energy consumption, it can realize synchronous production multiple product, agricultural straw resource is made to be maximally utilized, generate good economic benefit.
Description
Technical field
The present invention relates to technical field of biochemical industry, and in particular to a kind of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, oligomeric
The technique of xylose, lignin, cellulose fiber slurry.
Background technology
With the rapid development of economic society, resource consumption is getting faster, and resource scarcity, which has become, restricts global evolution
Tough questions.866,600,000,000 tons of plant organic matters are approximately formed on the earth every year, are that distributed in nature is most wide, content is most, price
Renewable resource that is cheap and being underutilized.It converts plant fiber waste to the energy by biotechnology, change
Chemical product, it is of far-reaching significance to solving energy crisis.
In recent years, grain can be saved at the technology of alcohol using microorganism enzymolysis stalk extraction polysaccharide and by polysaccharide conversion
Food can make full use of renewable resource again.However microorganism enzymolysis stalk products obtained therefrom low yield, water consume are high, high energy consumption, generate
A large amount of residues both increase production cost, also pollute the environment in this way.Therefore, exploitation can improve the warp of stalk enzymolysis
Ji benefit, and the production technology that stalk resource comprehensively utilizes can be promoted to be of great significance low-carbon economy development.
Invention content
In view of the deficienciess of the prior art, a kind of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol of present invention offer, oligomeric wood
The technique of sugar, lignin, cellulose fiber slurry.
The purpose of the present invention is achieved through the following technical solutions：
A kind of technique of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylo-oligosaccharide, lignin, cellulose fiber slurry, including
Following steps：
(1) pretreatment of raw material：Straw is kneaded into 4-8cm filiforms with wire kneading machine, stoking is without scleroma；
(2) it digests：By -02 series mixing of pretreated stalk and the cellulosic enzyme of corresponding straw type, water, pH is added
Then buffer solution is packaged with the double-deck material bag of sealing, indoor storage, hydrolysis temperature is 2-30 DEG C, and enzymolysis time is 7-150 days, pH
Value≤7；
(3) it detaches：Zymolyte is directly loadable into vacuum pulp washing machine, by squeezing, filtering, by liquid from cellulose slurry
It separates, liquid, which is sent into after lignin and cellulosic enzyme is separately recovered in micro-filtration and ultrafiltration apparatus, to be continued to produce ethyl alcohol and oligomeric wood
Sugar, cellulose slurry are sent into lower step and are screened, bleach and obtain cellulose fiber slurry；
(4) distillation, nanofiltration：Vapor is passed through in ethanol solution, controlled at 78.5-80 DEG C, by ethanol evaporation at
Gas, steam carry out dehydration purification through cooling reverse osmosis unit of being sent into；Product, that is, liquid glucose is sent into nanofiltration device and is taken off after distillation
Water detaches, then purified, drying process, produces finished product xylo-oligosaccharide；
(5) reverse osmosis purifying：By the water removal in ethanol solution, motive force is pressure difference, pressure be 1-10MPa to get
Alcohol fuel, alcohol fuel contain the impurity such as a small amount of methanol, can further rectifying removal.
Further, straw of the present invention is maize straw, broomcorn straw, wheat stalk, Barley straw, rice
One or more of stalk, grain straw.
Further, -02 series of plant straw fibers matter enzyme of the present invention is multi-functional complex enzyme, by cellulase,
The compositions such as hemicellulase, lignoenzyme, pectic substance enzyme, zymase, these enzyme components, which can cooperate with, destroys stalk institutional framework,
It is fiber micro wire, ethyl alcohol, xylose, polymeric lignin to make cellulose, hemicellulose, lignin degradation.Plant straw fibers matter enzyme
It is divided into 3 classes according to straw type, corn stalk fiber matter enzyme -02 is suitable for corn and broomcorn straw；Wheat straw fibre matter enzyme -02
Suitable for wheat, barley；Straw fibers matter enzyme -02 is suitable for rice, grain straw.
Under the action of plant straw fibers matter enzyme, stalk institutional framework decomposable process is：In the common of enzyme and hydrone
Under effect, first hydrone gradually penetrate into stalk tissue make stalk tissue generate expansion, make knot of tissue generate variation, tissue by
Close to become thin, water soluble pectin dissolving, this just enters substrate surface for enzyme and provides channel；Secondly as enzyme enters substrate surface,
The function that enzyme component begins to play hydrolysis substrate makes pectin object be hydrolyzed into solable matter, makes under the action of pectic substance enzyme
Histocyte detaches, until histocyte thoroughly becomes discrete state；In cellulase, hemicellulase, lignoenzyme, albumen
Under the synergistic effect of enzyme, the advanced labyrinth formed between cellulose, hemicellulose, lignin is destroyed, cellulose, half
Cellulose, lignin just become discrete state；Finally, in cellulose components, hemicellulase component, lignoenzyme component
Under effect, corresponding substrate is further hydrolyzed to fiber micro wire, glucose, xylo-oligosaccharide, lignin, and glucose is in zymase
Under the action of be converted into ethyl alcohol.
The work of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol provided by the invention, xylo-oligosaccharide, lignin, cellulose fiber slurry
Skill organically combines bioenzymatic conversion technology and membrane separation technique, and agricultural straw resource is made to be maximally utilized.Do not changing
In the case of becoming technique, equipment, using maize straw, Wheat Straw, rice straw as raw material, researched and developed for the characteristics of each stalk
Adaptable cellulosic enzyme therewith, makes stalk active principle limitization utilize, and synchronous production multiple product greatly reduces technique
Cost, overcome existing stalk enzymolysis time is long, difficulty is big, product is single, water consumption, energy consumption problem.
The membrane separation technique that the present invention uses has separation, concentration, purifying and refined function, energy-efficient, environmentally friendly, filtering
Process is simple, easily controllable；It carries out at normal temperatures, only electric energy is needed to push, energy consumption is about the 1/3-1/8 being concentrated by evaporation；It is allusion quotation
The physical separating process of type, does not have to chemical reagent, and product is not contaminated.
The present invention has the advantages that compared with prior art：
(1) stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol provided by the invention, xylo-oligosaccharide, lignin, cellulose fiber slurry
Technique compared with existing stalk enzymolysis process, using cellulose material enzyme corresponding with straw type, with marketed cellulose enzyme phase
Than enzymatic hydrolysis condition is mild, only needs room temperature that can carry out, and enzymolysis efficiency is high, can destroy the labyrinth of stalk, stalk is without multiple
Miscellaneous pretreatment, packing storage can carry out enzymolysis process, simplify operation, saved equipment cost, overcome enzymatic hydrolysis and fermentation week
Equipment cost caused by phase is grown is big and the limited defect of high energy consumption, scale；
(2) stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol provided by the invention, xylo-oligosaccharide, lignin, cellulose fiber slurry
Technique use membrane separation technique, simple for process, easy to operate, the degree of automation is higher, and low energy consumption, pollution-free, Neng Goushi
The recycling of existing cellulose material enzyme, greatly reduces enzyme cost；
(3) stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol provided by the invention, xylo-oligosaccharide, lignin, cellulose fiber slurry
Technique, can high-volume continuous production, technological flexibility is high, and cellulose, hemicellulose, the lignin in stalk are totally converted
At production product, ethyl alcohol and xylo-oligosaccharide high conversion rate, hemicellulose utilization rate is high, and cellulose slurry can not only be used for regenerated fiber original
Production material also can be used as and make low raw material, good in economic efficiency, to comprehensive utilization of resources and energy-efficient be of great significance.
Description of the drawings
Fig. 1 is the stream of the technique of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylo-oligosaccharide, lignin, cellulose fiber slurry
Cheng Tu.
Specific implementation mode
Further to illustrate the technological means and its effect of the invention taken, below in conjunction with drawings and the specific embodiments
It is described in detail.
Embodiment 1
A kind of technique (Fig. 1 institutes of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylo-oligosaccharide, lignin, cellulose fiber slurry
Show), include the following steps：
(1) pretreatment of raw material：Maize straw, broomcorn straw are kneaded into 4-8cm filiforms with wire kneading machine, stoking is without scleroma；
(2) it digests：1kg corn stalk fiber matter enzyme-is added by the pretreated maize straws of every 200kg, broomcorn straw
02 is uniformly mixed, and is then respectively adding water, pH buffer solutions, is then packaged with the double-deck material bag of sealing, indoor storage, and hydrolysis temperature is
2-30 DEG C, enzymolysis time is 7-150 days, pH value≤7；
(3) it detaches：After the completion of enzymolysis, zymolyte is directly loadable into vacuum pulp washing machine, by squeezing, filtering, by liquid
It is separated from cellulose slurry, after liquid is sent into micro-filtration extraction lignin, is re-fed into ultrafiltration apparatus recycled fiber matter enzyme
After continue to produce ethyl alcohol and xylo-oligosaccharide, cellulose slurry is sent into lower step and screen to be starched up to essential color fiber cellulose fiber；It is sieved
It selects, bleach and starched up to bleached cellulose fibres；
(4) distillation, nanofiltration：Vapor is passed through in ethanol solution, controlled at 78.5-80 DEG C, by ethanol evaporation at
Gas, steam carry out dehydration purification through cooling reverse osmosis unit of being sent into；Product, that is, liquid glucose is sent into nanofiltration device and is taken off after distillation
Water detaches, then purified, drying process, produces finished product xylo-oligosaccharide；
(5) reverse osmosis purifying：By the water removal in ethanol solution, motive force is pressure difference, pressure be 1-10MPa to get
Alcohol fuel, alcohol fuel contain the impurity such as a small amount of methanol, can further rectifying removal.
Further, corn stalk fiber matter enzyme -02 of the present invention is multi-functional complex enzyme, is 2 by quality proportioning:4:
1:1:The compositions such as 2 cellulase, hemicellulase, lignoenzyme, pectic substance enzyme, zymase, these enzyme components can cooperate with
Destroy maize straw, broomcorn straw institutional framework, make cellulose, hemicellulose, lignin degradation be fiber micro wire, ethyl alcohol,
Xylo-oligosaccharide, polymeric lignin.
Embodiment 2
A kind of technique (Fig. 1 institutes of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylo-oligosaccharide, lignin, cellulose fiber slurry
Show), include the following steps：
(1) pretreatment of raw material：Barley straw, wheat stalk are kneaded into 4-8cm filiforms with wire kneading machine respectively, stoking is without hard
Knot；
(2) it digests：1.3kg wheat straw fibre matter enzyme-is added by the pretreated Barley straws of every 200kg, wheat stalk
02 is uniformly mixed, and is then respectively adding water, pH buffer solutions, is then packaged with the double-deck material bag of sealing, indoor storage, and hydrolysis temperature is
2-30 DEG C, enzymolysis time is 7-150 days, pH value≤7；
(3) it detaches：After the completion of enzymolysis, zymolyte is directly loadable into vacuum pulp washing machine, by squeezing, filtering, by liquid
It is separated from cellulose slurry, after liquid is sent into micro-filtration extraction lignin, is re-fed into ultrafiltration apparatus recycled fiber matter enzyme
After continue to produce ethyl alcohol and xylo-oligosaccharide, cellulose slurry is sent into lower step and screen to be starched up to essential color fiber cellulose fiber；It is sieved
It selects, bleach and starched up to bleached cellulose fibres；
(4) distillation, nanofiltration：Vapor is passed through in ethanol solution, controlled at 78.5-80 DEG C, by ethanol evaporation at
Gas, steam carry out dehydration purification through cooling reverse osmosis unit of being sent into；Product, that is, liquid glucose is sent into nanofiltration device and is taken off after distillation
Water detaches, then purified, drying process, produces finished product xylo-oligosaccharide；
(5) reverse osmosis purifying：By the water removal in ethanol solution, motive force is pressure difference, pressure be 1-10MPa to get
Alcohol fuel, alcohol fuel contain the impurity such as a small amount of methanol, can further rectifying removal.
Further, wheat straw fibre matter enzyme -02 of the present invention is multi-functional complex enzyme, is 4 by quality proportioning:3:1:
1:The compositions such as 4 cellulase, hemicellulase, lignoenzyme, pectic substance enzyme, zymase, these enzyme components can cooperate with brokenly
The institutional framework of bad Barley straw, wheat stalk makes cellulose, hemicellulose, lignin degradation be fiber micro wire, is ethyl alcohol, low
Xylan, polymeric lignin.
Embodiment 3
A kind of technique (Fig. 1 institutes of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylo-oligosaccharide, lignin, cellulose fiber slurry
Show), include the following steps：
(1) pretreatment of raw material：Rice straw, grain straw are kneaded into 4-8cm filiforms with wire kneading machine respectively, stoking is without hard
Knot；
(2) it digests：1.3kg paddy stalk fibre matter is added by the pretreated rice straws of every 200kg, grain straw
Enzyme -02 is uniformly mixed, and is then respectively adding water, pH buffer solutions, is then packaged with the double-deck material bag of sealing, indoor storage, enzymolysis temperature
Degree is 2-30 DEG C, and enzymolysis time is 7-150 days, pH value≤7；
(3) it detaches：After the completion of enzymolysis, zymolyte is directly loadable into vacuum pulp washing machine, by squeezing, filtering, by liquid
It is separated from cellulose slurry, after liquid is sent into micro-filtration extraction lignin, is re-fed into ultrafiltration apparatus recycled fiber matter enzyme
After continue to produce ethyl alcohol and xylo-oligosaccharide, cellulose slurry is sent into lower step and screen to be starched up to essential color fiber cellulose fiber；It is sieved
It selects, bleach and starched up to bleached cellulose fibres；
(4) distillation, nanofiltration：Vapor is passed through in ethanol solution, controlled at 78.5-80 DEG C, by ethanol evaporation at
Gas, steam carry out dehydration purification through cooling reverse osmosis unit of being sent into；Product, that is, liquid glucose is sent into nanofiltration device and is taken off after distillation
Water detaches, then purified, drying process, produces finished product xylo-oligosaccharide；
(5) reverse osmosis purifying：By the water removal in ethanol solution, motive force is pressure difference, pressure be 1-10MPa to get
Alcohol fuel, alcohol fuel contain the impurity such as a small amount of methanol, can further rectifying removal.
Further, paddy stalk fibre matter enzyme -02 of the present invention is multi-functional complex enzyme, is 3 by quality proportioning:5:
1:1:The compositions such as 3 cellulase, hemicellulase, lignoenzyme, pectic substance enzyme, zymase, these enzyme components can cooperate with
Destroy rice straw, grain straw institutional framework, make cellulose, hemicellulose, lignin degradation be fiber micro wire, ethyl alcohol,
Xylo-oligosaccharide, polymeric lignin.
Embodiment 4
A kind of technique (Fig. 1 institutes of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylo-oligosaccharide, lignin, cellulose fiber slurry
Show), include the following steps：
(1) pretreatment of raw material：By maize straw, broomcorn straw, wheat stalk, Barley straw, oat straw, rice straw
Stalk, grain straw are kneaded into 4-8cm filiforms with wire kneading machine respectively, and stoking is without scleroma；
(2) it digests：- 02 series of 1.3kg cellulosics enzyme is added by the pretreated straws of every 200kg to be uniformly mixed,
It is then respectively adding water, pH buffer solutions, is then packaged with the double-deck material bag of sealing, indoor storage, hydrolysis temperature is 2-30 DEG C, enzymolysis
Time is 7-150 days, pH value≤7；
(3) it detaches：After the completion of enzymolysis, zymolyte is directly loadable into vacuum pulp washing machine, by squeezing, filtering, by liquid
It is separated from cellulose slurry, after liquid is sent into micro-filtration extraction lignin, is re-fed into ultrafiltration apparatus recycled fiber matter enzyme
After continue to produce ethyl alcohol and xylo-oligosaccharide, cellulose slurry is sent into lower step and screen to be starched up to essential color fiber cellulose fiber；It is sieved
It selects, bleach and starched up to bleached cellulose fibres；
(4) distillation, nanofiltration：Vapor is passed through in ethanol solution, controlled at 78.5-80 DEG C, by ethanol evaporation at
Gas, steam carry out dehydration purification through cooling reverse osmosis unit of being sent into；Product, that is, liquid glucose is sent into nanofiltration device and is taken off after distillation
Water detaches, then purified, drying process, produces finished product xylo-oligosaccharide；
(5) reverse osmosis purifying：By the water removal in ethanol solution, motive force is pressure difference, pressure be 1-10MPa to get
Alcohol fuel, alcohol fuel contain the impurity such as a small amount of methanol, can further rectifying removal.
Further, -02 series of plant fiber matter enzyme of the present invention is multi-functional complex enzyme, corn stalk fiber used
Matter enzyme -01 is 2 by quality proportioning:4:1:1:2 cellulase, hemicellulase, lignoenzyme, pectic substance enzyme, zymase etc.
Composition；Wheat straw fibre matter enzyme -02 is 4 by quality proportioning:3:1:1:4 cellulase, hemicellulase, lignoenzyme, fruit
The compositions such as colloid enzyme, zymase；Paddy stalk fibre matter enzyme -02 is 3 by quality proportioning:5:1:1:3 cellulase, hemicellulose
The compositions such as plain enzyme, lignoenzyme, pectic substance enzyme, zymase；These enzyme components can cooperate with the institutional framework for destroying corresponding stalk,
It is fiber micro wire, ethyl alcohol, xylo-oligosaccharide, polymeric lignin to make all kinds of stalk institutes containing cellulose, hemicellulose, lignin degradation.
Comparative example 1
In addition to enzyme used is marketed cellulose enzyme, remaining is the same as embodiment 1.
Comparative example 2
In addition to enzyme used is marketed cellulose enzyme, remaining is the same as embodiment 2.
Comparative example 3
In addition to enzyme used is marketed cellulose enzyme, remaining is the same as embodiment 3.
Comparative example 4
In addition to enzyme used is marketed cellulose enzyme, remaining is the same as embodiment 4.
Interpretation of result
1~4 products obtained therefrom yield of Examples 1 to 4 and comparative example is shown in Table 1 (per 200kg stalks).
1 unit of table：kg
| Product type | Ethyl alcohol | Xylo-oligosaccharide | Lignin | Cellulose fiber slurry |
| Embodiment 1 | 15.8 | 58.66 | 7.87 | 32.90 |
| Embodiment 2 | 20.74 | 38.30 | 16.25 | 43.20 |
| Embodiment 3 | 19.02 | 58.65 | 10.77 | 39.60 |
| Embodiment 4 | 18.6 | 52.01 | 11.75 | 38.64 |
| Comparative example 1 | 3.04 | 1.42 | - | - |
| Comparative example 2 | 3.22 | 1.14 | - | - |
| Comparative example 3 | 3.19 | 1.59 | - | - |
| Comparative example 4 | 3.27 | 1.37 | - | - |
As can be seen from the above data, stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, the wood of the offer of the embodiment of the present invention 1~4
Sugar, lignin, cellulose fiber slurry the yield of technique products obtained therefrom be significantly larger than comparative example 1~4, show provided by the invention
The serial complex enzyme of plant straw fibers matter enzyme -01 is adapted with corresponding straw type and production product category, can be targeted
Ground destroys the composed structure of stalk, improves enzymolysis efficiency, and stalk only needs simple and mechanical pretreatment, and hydrolysis result is good, and stalk is effective
Ingredient is fully used, and product utilization rate is high, good in economic efficiency.And marketed cellulose enzyme cannot destroy stalk institutional framework,
Product yield is extremely low.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, ability
Other modifications or equivalent replacement that domain those of ordinary skill makes technical scheme of the present invention, without departing from skill of the present invention
The spirit and scope of art scheme, are intended to be within the scope of the claims of the invention.
Claims (5)
1. the technique of a kind of stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol, xylo-oligosaccharide, lignin, cellulose fiber slurry, feature
It is, includes the following steps：
（1）Pretreatment of raw material：Straw is kneaded into 4-8 cm filiforms with wire kneading machine, stoking is without scleroma；
（2）Enzymolysis：By -02 series mixing of pretreated stalk and the cellulosic enzyme of corresponding straw type, water, pH bufferings is added
Then liquid is packaged, indoor storage, enzymolysis with the double-deck material bag of sealing；
（3）Separation：Zymolyte is directly loadable into vacuum pulp washing machine, by squeezing, filtering, liquid is detached from cellulose slurry
Out, after liquid is sent into micro-filtration extraction lignin, be re-fed into after ultrafiltration apparatus recycled fiber matter enzyme continue to produce ethyl alcohol and
Xylo-oligosaccharide, cellulose slurry are sent into lower step and are screened, bleach and obtain cellulose fiber slurry；
（4）Distillation, nanofiltration：Vapor is passed through in ethanol solution, by ethanol evaporation at gas, steam is reverse osmosis through cooling feeding
Device carries out dehydration purification；Product, that is, liquid glucose is sent into nanofiltration device and carries out dehydration separation, then purified, drying process after distillation,
Produce finished product xylo-oligosaccharide；
（5）Reverse osmosis purifying：By the water removal in ethanol solution, motive force is pressure difference, and pressure is 1-10 MPa to get fuel
Ethyl alcohol, alcohol fuel contain the impurity such as a small amount of methanol, can further rectifying removal.
2. stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol according to claim 1, xylo-oligosaccharide, lignin, cellulose fibre
The technique of slurry, which is characterized in that the straw is maize straw, broomcorn straw, wheat stalk, Barley straw, rice straw
One or more of stalk, grain straw.
3. stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol according to claim 1, xylo-oligosaccharide, lignin, cellulose fibre
The technique of slurry, which is characterized in that -02 series of the plant straw fibers matter enzyme is multi-functional complex enzyme, and enzyme includes cellulose
Enzyme, hemicellulase, lignoenzyme, pectic substance enzyme, protease, zymase.
4. stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol according to claim 1, xylo-oligosaccharide, lignin, cellulose fibre
The technique of slurry, which is characterized in that step（2）Middle hydrolysis temperature is 2-30 DEG C, and enzymolysis time is 7-150 days, pH value≤7.
5. stalk enzymatic hydrolysis and fermentation synchronous production ethyl alcohol according to claim 1, xylo-oligosaccharide, lignin, cellulose fibre
The technique of slurry, which is characterized in that step（4）Middle ethanol evaporation process control temp is 78.5-80 DEG C.
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| CN109988699A (en) * | 2019-02-25 | 2019-07-09 | 车团结 | Stalk enzymatic hydrolysis and fermentation prepares the feedstock composition and its methods and applications of vinegar |
| CN110904159A (en) * | 2019-12-27 | 2020-03-24 | 孟保奎 | Method for co-producing feruloyl oligosaccharide and ethanol |
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