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CN108782768A - A kind of fermented tea ferments full beans soya-bean milk and its production method - Google Patents

A kind of fermented tea ferments full beans soya-bean milk and its production method Download PDF

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CN108782768A
CN108782768A CN201810423936.3A CN201810423936A CN108782768A CN 108782768 A CN108782768 A CN 108782768A CN 201810423936 A CN201810423936 A CN 201810423936A CN 108782768 A CN108782768 A CN 108782768A
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fermented
bean milk
fermented tea
fermentation
tea
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夏秀东
周剑忠
王英
刘小莉
单成俊
李莹
张丽霞
范琳琳
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Jiangsu Yanjiang Agricultural Science Research Institute
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C11/00Milk substitutes, e.g. coffee whitener compositions
    • A23C11/02Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
    • A23C11/10Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
    • A23C11/103Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins containing only proteins from pulses, oilseeds or nuts, e.g. nut milk
    • A23C11/106Addition of, or treatment with, microorganisms

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  • Beans For Foods Or Fodder (AREA)
  • Dairy Products (AREA)

Abstract

本发明公开一种红茶菌发酵全豆豆乳及其生产方法,大豆经清洗、浸泡、粗粉碎、胶体磨粉碎、高压均质、巴氏灭菌后得到灭菌全豆豆乳,然后加入红茶菌发酵种子后于适宜条件下发酵,最后经过后熟得到红茶菌发酵全豆豆乳成品。本发明提供了一种以全豆为原料的红茶菌发酵全豆豆乳生产方法,不仅实现了全豆的全价利用,保留了大豆全部营养和保健物质,不产生任何副产物,且经红茶菌发酵的全豆豆乳豆腥味大大改善,胀气因子大大降低,生成了大量的游离氨基酸、苷元型大豆异黄酮、葡萄糖醛酸等营养保健物质,增强了豆乳的营养保健功能,同时本发明设备要求低,生产投入少,可大大降低生产成本,拥有广阔的市场前景。

The invention discloses a kombucha fermented whole bean soymilk and a production method thereof. After soybeans are cleaned, soaked, coarsely crushed, colloid mill crushed, high-pressure homogenized and pasteurized, sterilized whole bean soymilk is obtained, and then kombucha is added for fermentation After the seeds are fermented under suitable conditions, the finished product of kombucha-fermented whole soy bean milk can be obtained through post-ripening. The invention provides a method for producing soybean milk fermented by kombucha fungus from whole beans, which not only realizes the full price utilization of whole beans, but also retains all nutrition and health care substances of soybeans without producing any by-products. The fermented whole soy milk has a greatly improved beany smell, a greatly reduced flatulence factor, and a large amount of free amino acids, aglycon-type soybean isoflavones, glucuronic acid and other nutritional and health-care substances are produced, which enhances the nutritional and health-care functions of the soy milk. At the same time, the device of the present invention Low requirements and low production input can greatly reduce production costs and have broad market prospects.

Description

一种红茶菌发酵全豆豆乳及其生产方法A kind of kombucha fungus fermented whole soybean milk and its production method

技术领域technical field

本发明涉及一种红茶菌发酵全豆豆乳及其生产方法,属于豆制品深加工领域。The invention relates to kombucha fermented whole soybean milk and a production method thereof, belonging to the field of deep processing of soybean products.

背景技术Background technique

大豆具有丰富的营养成分而被广泛地用于制作各种豆类加工制品。大豆中蛋白质含量约为40%,最高可达到50%,与其他农作物如小麦,玉米及大米相比高出3-6倍。同时大豆蛋白中除蛋氨酸含量稍低外,其他氨基酸含量丰富,是植物性的完全蛋白质,其组成和鸡蛋牛奶相近,营养价值相当高,可以有效解决发达国家摄入过多的动物性蛋白导致肥胖的问题。大豆中含有约15%-20%的脂肪,其中85%是人体必需的脂肪酸,亚油酸含量在50%以上,有益于人体健康。除此之外,大豆中还含有多种维生素,糖类,矿物质,以及天然的功能性活性成分。多食大豆可以有效预防心血管,老年痴呆,脂肪肝,肝硬化等多种疾病,大豆中的异黄酮还具有抗氧化,防衰老,降低胆固醇,预防骨质疏松等疾病的功效。Soybeans are rich in nutrients and are widely used to make various processed soy products. The protein content in soybean is about 40%, up to 50%, which is 3-6 times higher than that of other crops such as wheat, corn and rice. At the same time, except for the slightly low methionine content in soybean protein, other amino acids are rich in content. It is a complete plant-based protein. Its composition is similar to that of eggs and milk, and its nutritional value is quite high. It can effectively solve the problem of obesity caused by excessive intake of animal protein in developed countries. The problem. Soybeans contain about 15%-20% fat, 85% of which are essential fatty acids for the human body, and the content of linoleic acid is more than 50%, which is beneficial to human health. In addition, soybeans also contain a variety of vitamins, sugars, minerals, and natural functional active ingredients. Eating more soybeans can effectively prevent cardiovascular diseases, Alzheimer's disease, fatty liver, liver cirrhosis and other diseases. The isoflavones in soybeans also have the effects of anti-oxidation, anti-aging, lowering cholesterol, and preventing osteoporosis and other diseases.

发酵豆乳最早在20世纪初的欧洲出现,这也是在西方起源的唯一一种传统豆制品。某些益生菌可以在牛乳中生长,并应用于乳品中,生产出各类发酵乳制品。在微生物的作用下,发酵后的豆乳不但延长了保质期,品质和口味均有改变,使得乳制品变得更好吃、更好喝。大豆中除了有些特殊风味外,其余都与牛奶比较接近。进行豆乳发酵处理是豆浆延长货架期的一种有效的方法,而且对豆乳的质构和风味有了调整。与豆乳相比,发酵豆乳好处极多,发酵豆乳中保留了豆乳原有的营养成分,在微生物发酵作用下豆乳中的抗营养因子被分解,进一步提高了豆乳的营养价值,且活菌及其代谢产物还能促进人体肠道有益菌群的生长,因此,酸豆乳拥有比豆乳更高的营养价值和保健功能。微生物在生长、代谢过程中不仅能够有效降低豆乳中的豆腥味,还能产生醇香、清爽的酸奶香味,因此,相较于豆乳,酸豆乳的风味品质具有更为广泛的接受度(尤其是对豆腥味十分敏感的西方消费者)。益生菌还能利用豆乳中的碳源产酸,赋予酸豆乳细腻的凝乳质地和酸甜滑润的口感。而且发酵完成后胀气因子有所降解,不与微生物作用产气,也就没有了胃胀现象;维生素和大豆异黄酮等营养成分都有提高;由于发酵豆乳中不含有乳糖,乳糖不耐症人群也可食用。此外发酵后的豆乳蛋白质消化率达到95%,对于人体利用有益。可见,酸豆乳优良的营养特性和独特的产品形式显现出巨大的开发价值以及广阔的市场前景。Fermented soy milk first appeared in Europe in the early 20th century, and it is the only traditional soy product that originated in the West. Certain probiotics can grow in milk and are used in dairy products to produce various fermented dairy products. Under the action of microorganisms, the shelf life of fermented soy milk is not only extended, but also the quality and taste are changed, making dairy products more delicious and delicious. Except for some special flavors in soybeans, the rest are closer to milk. Fermentation of soymilk is an effective method to prolong the shelf life of soymilk, and the texture and flavor of soymilk have been adjusted. Compared with soymilk, fermented soymilk has many benefits. Fermented soymilk retains the original nutrients of soymilk, and the anti-nutritional factors in soymilk are decomposed under the action of microbial fermentation, which further improves the nutritional value of soymilk, and live bacteria and its Metabolites can also promote the growth of beneficial bacteria in the human intestinal tract. Therefore, sour soy milk has higher nutritional value and health care functions than soy milk. During the growth and metabolism of microorganisms, not only can effectively reduce the beany smell in soy milk, but also produce a mellow and refreshing yogurt aroma. Therefore, compared with soy milk, the flavor quality of soy milk is more widely accepted (especially Western consumers who are very sensitive to beany smell). Probiotics can also use the carbon source in soy milk to produce acid, giving soy milk a fine curd texture and a sweet and sour taste. Moreover, after the fermentation is completed, the flatulence factor is degraded, and it does not interact with microorganisms to produce gas, so there is no bloating phenomenon; vitamins and soybean isoflavones and other nutrients are improved; since fermented soy milk does not contain lactose, lactose intolerant people Also edible. In addition, the protein digestibility of fermented soymilk reaches 95%, which is beneficial for human body utilization. It can be seen that the excellent nutritional properties and unique product form of sour soy milk have shown great development value and broad market prospects.

传统豆制品加工中的制浆工艺,多去皮去渣,造成资源的浪费,同时也对环境造成了污染。研究表明,豆渣中膳食纤维占50%~70%,蛋白质含量占19%~23%,脂肪含量8%~11%,但是由于豆渣口感粗糙,目前大部分豆渣被当作动物饲料、肥料,甚至废弃物直接扔掉。豆渣膳食纤维含量高且生产成本较低,可将其加工成一种高膳食纤维粉体,作为一种膳食纤维补充剂来预防糖尿病、肥胖症、心血管疾病等;也可以作为一种食品原料,在提高产品品质的同时,减少感官影响。因此开发以全豆豆浆为原料制备发酵豆乳,对大豆进行全利用,不仅能实现全豆的全价利用,还能避免了对环境的污染。The pulping process in the traditional soybean product processing often removes the skin and slag, which causes a waste of resources and also pollutes the environment. Studies have shown that the dietary fiber in bean dregs accounts for 50% to 70%, the protein content accounts for 19% to 23%, and the fat content is 8% to 11%. However, due to the rough taste of bean dregs, most of them are currently used as animal feed, fertilizer, or even The waste is thrown away directly. Okara has high dietary fiber content and low production cost. It can be processed into a high dietary fiber powder as a dietary fiber supplement to prevent diabetes, obesity, cardiovascular disease, etc.; it can also be used as a food raw material, While improving product quality, reduce sensory impact. Therefore, the development of fermented soymilk using whole soybean soymilk as raw material and full utilization of soybeans can not only realize the full price utilization of whole beans, but also avoid environmental pollution.

红茶菌是有着悠久历史的一种民间传统酸性发酵饮料,它主要是是酵母菌、醋酸菌和乳酸菌以糖茶水为原料的微生物发酵饮料,味道酸甜可口,是一种纯天然保健饮品。红茶菌的菌膜酷似海蜇皮,故又称为“海宝”,由于它能帮助消化,对多种胃病有一定的医疗保健作用,所以在有些地方又称其为“胃宝”。Kombucha is a folk traditional acidic fermented drink with a long history. It is mainly a microbial fermented drink made of yeast, acetic acid bacteria and lactic acid bacteria using sugar tea as raw material. It tastes sweet and sour, and is a pure natural health drink. The pellicle of kombucha fungus resembles jellyfish skin, so it is also called "haibao". Because it can help digestion and has certain medical and health care effects on various stomach diseases, it is also called "stomach treasure" in some places.

人们之所以如此广泛的喜欢饮用红茶菌,不仅因为红茶菌清香可口、制作简便,更重要的是它还具有多种保健功能。培养成熟的红茶菌液中含有多种对人体健康有益的营养成分,比如醋酸、葡萄糖酸、葡萄糖、果糖、氨基酸、蛋白质、维生素、咖啡因、茶多酚、乙醇、维生素及二氧化碳等化合物及微量元素,因此,它被认为是一种具有多种保健功能的健康饮品,它的保健功能有:清理肠道,预防和治疗便秘及痔疮;帮助消化;可以预防和治疗高血脂、高血压、动脉硬化等心脑血管疾病及糖尿病;还可以预防各种结石的发生;有助于身体及时排除体内毒素,防癌、抗癌并增强机体免疫力等。The reason why people like drinking kombucha so widely is not only because kombucha is delicious and easy to make, but more importantly, it also has multiple health care functions. The mature kombucha liquid contains a variety of nutrients beneficial to human health, such as acetic acid, gluconic acid, glucose, fructose, amino acids, proteins, vitamins, caffeine, tea polyphenols, ethanol, vitamins and carbon dioxide and other compounds and trace amounts element, therefore, it is considered to be a health drink with multiple health functions. Its health functions include: cleaning the intestinal tract, preventing and treating constipation and hemorrhoids; helping digestion; preventing and treating hyperlipidemia, high blood pressure, arterial Cardiovascular and cerebrovascular diseases such as sclerosis and diabetes; it can also prevent the occurrence of various stones; it helps the body to eliminate toxins in time, prevent cancer, fight cancer and enhance the body's immunity.

本发明利用红茶菌发酵全豆豆乳,不仅实现了大豆的全价利用,最大程度的保留了大豆中的成分,而且赋予了发酵豆乳更多的营养和保健功能,改善了豆乳的风味,是一种具有广阔市场前景的发酵豆制品。The present invention uses kombucha to ferment whole soybean milk, not only realizes the full price utilization of soybeans, retains the ingredients in soybeans to the greatest extent, but also endows fermented soybean milk with more nutrition and health care functions, and improves the flavor of soybean milk. A fermented bean product with broad market prospects.

发明内容Contents of the invention

发明目的:本发明针对传统豆乳生产方式会产生大量豆渣副产物,不仅使大豆利用率降低,造成大豆营养保健物质流失,而且不合理利用会造成环境污染,以及豆乳豆腥味重,发酵豆制品品种单一,消费者选择余地较小等问题,提供了一种以全豆为原料的红茶菌发酵全豆豆乳生产方法,不仅实现了全豆的全价利用,保留了大豆全部营养和保健物质,不产生任何副产物,而且经红茶菌发酵的全豆豆乳豆腥味大大改善,胀气因子大大降低,生成了大量的游离氨基酸、苷元型大豆异黄酮、葡萄糖醛酸等营养保健物质,增强了豆乳的营养保健功能。Purpose of the invention: The present invention aims at producing a large amount of by-products of bean dregs in the traditional soymilk production method, which not only reduces the utilization rate of soybeans, causes the loss of soybean nutrition and health care substances, but also causes environmental pollution due to unreasonable use, and the beany smell of soymilk is heavy, and fermented soy products For the problems of single variety and limited choice for consumers, a production method of kombucha fermented whole soybean milk with whole soybeans as raw material is provided, which not only realizes the full price utilization of whole soybeans, but also retains all the nutrients and health-care substances of soybeans, No by-products are produced, and the beany smell of whole soy milk fermented by kombucha is greatly improved, the flatulence factor is greatly reduced, and a large amount of free amino acids, aglycon-type soybean isoflavones, glucuronic acid and other nutritional and health substances are produced, which enhances the Nutrition and health function of soybean milk.

为了解决上述技术问题,本发明公开了一种红茶菌发酵全豆豆乳及其生产方法,包括如下步骤:In order to solve the above-mentioned technical problems, the present invention discloses a kombucha-fermented whole soybean milk and a production method thereof, comprising the following steps:

(1)将大豆清洗干净后按照豆水质量比为1∶6-8的比例将大豆浸泡于水中8-24小时;(1) Soak the soybeans in water for 8-24 hours according to the ratio of soybean water mass ratio of 1:6-8 after cleaning the soybeans;

(2)将浸泡好的大豆和水一起先用粗粉碎机粉碎,然后加入胶体磨中粉碎2-3次后再用高压均质机均质2-3次得到全豆豆乳;(2) The soaked soybeans and water are first pulverized with a coarse pulverizer, then added to a colloid mill and pulverized 2-3 times, and then homogenized 2-3 times with a high-pressure homogenizer to obtain whole soybean milk;

(3)全豆豆乳巴氏灭菌、冷却至室温后得到灭菌全豆豆乳;(3) Whole soy milk is pasteurized and cooled to room temperature to obtain sterilized whole soy milk;

(4)将红茶或绿茶、蔗糖和水按照质量比为0.3-0.8∶5-8∶100的比例混合、搅拌,蔗糖充分溶解后加在95-100℃条件下加热5-10分钟,冷却,滤掉茶叶,得到糖茶水。(4) Mix and stir black tea or green tea, sucrose and water according to the mass ratio of 0.3-0.8:5-8:100. After the sucrose is fully dissolved, heat it at 95-100°C for 5-10 minutes and cool it down. Filter out the tea leaves to obtain sugar tea water.

(5)将购自市售的红茶菌或利用醋酸菌、酵母菌和乳酸菌纯菌混合发酵的红茶菌(制备方法参照发明专利:利用纯菌混合发酵生产红茶菌饮料,专利号为:CN101491362B)加入到配好的糖茶水中发酵3天以上,得到红茶菌发酵种子;(5) Kombucha purchased from the market or kombucha mixed with acetic acid bacteria, saccharomyces and lactic acid bacteria (for the preparation method, refer to the invention patent: production of kombucha beverage by mixed fermentation of pure bacteria, patent number: CN101491362B) Add it to the prepared sugar tea water and ferment for more than 3 days to obtain kombucha fermented seeds;

(6)步骤(5)所得红茶菌发酵种子加入步骤(3)所得灭菌全豆豆乳中于适宜发酵温度下发酵得到红茶菌发酵全豆豆乳;(6) adding the kombucha fermented seeds obtained in step (5) to the sterilized whole soybean milk obtained in step (3) and fermenting at a suitable fermentation temperature to obtain kombucha fermented whole soybean milk;

(7)红茶菌发酵全豆豆后熟后得到红茶菌发酵全豆豆乳成品。(7) After ripening the whole beans fermented by kombucha fungus, the whole soybean milk product fermented by kombucha fungus is obtained.

步骤(2)中,所述高压均质为两段式高压均质,温度为60-65℃,第一段均质压强为25~30MPa,第二段均质压强为4.0~4.5MPa。In step (2), the high-pressure homogenization is a two-stage high-pressure homogenization, the temperature is 60-65° C., the homogenization pressure of the first stage is 25-30 MPa, and the homogenization pressure of the second stage is 4.0-4.5 MPa.

步骤(3)中,所述巴氏杀菌条件为80-90℃,20-30分钟。In step (3), the pasteurization condition is 80-90° C. for 20-30 minutes.

步骤(5)中,所述红茶菌发酵种子为红茶菌发酵液或红茶菌菌膜或红茶菌发酵液和红茶菌菌膜任意比例的混合物。In step (5), the kombucha fermented seeds are kombucha fermentation liquid or kombucha pellicle or a mixture of kombucha fermentation liquid and kombucha pellicle in any proportion.

步骤(6)中,红茶菌发酵种子的添添加量为全豆豆乳质量的5-30%;发酵温度为25-35℃;发酵时间为1-3天;发酵终点判断依据为红茶菌发酵全豆豆乳的pH为4.2-4.6。In step (6), the amount of kombucha fermented seeds is 5-30% of the mass of whole soybean milk; the fermentation temperature is 25-35°C; the fermentation time is 1-3 days; The pH of soy milk is 4.2-4.6.

步骤(7)中,所述的后熟条件为温度为2-6℃,时间为4-8小时In step (7), the post-ripening condition is that the temperature is 2-6°C and the time is 4-8 hours

以上任意一种制备方法制备得到的蓝莓奶啤饮品也在本发明的保护范围中。The blueberry milk beer drink prepared by any one of the above preparation methods is also within the protection scope of the present invention.

有益效果:Beneficial effect:

1、本申请以全豆为原料,实现了大豆的全价利用,不产生任何副产物,最大程度的保留了大豆中的所有营养和保健物质,是一种绿色、健康的发酵豆乳生产技术。1. This application uses whole soybeans as raw materials, realizes the full price utilization of soybeans, does not produce any by-products, and retains all the nutrients and health-care substances in soybeans to the greatest extent. It is a green and healthy fermented soybean milk production technology.

2、本申请创造性的利用红茶菌发酵全豆豆乳,大大改善了豆乳的风味,降低了豆腥味,又能赋予豆乳更多的游离氨基酸、维生素、苷元型大豆异黄酮、葡萄糖醛酸等营养保健物质。2. This application creatively uses kombucha to ferment whole soy milk, which greatly improves the flavor of soy milk, reduces the beany smell, and can endow soy milk with more free amino acids, vitamins, aglycon-type soybean isoflavones, glucuronic acid, etc. Nutrition and health substances.

3、本申请工艺简单,生产设备均为常见设备,普通奶制品和豆制品生产厂家只需增购简单设备即可实现生产,新厂家购置设备价格低廉,可大大减少企业投入,降低企业生产成本,增加企业利润。3. The process of this application is simple, and the production equipment is common equipment. Ordinary dairy and bean product manufacturers only need to purchase simple equipment to realize production. New manufacturers purchase equipment at a low price, which can greatly reduce enterprise investment and production costs. , increase corporate profits.

附图说明Description of drawings

下面结合附图和具体实施方式对本发明做更进一步的具体说明,本发明的上述和/或其他方面的优点将会变得更加清楚。The advantages of the above and/or other aspects of the present invention will become clearer as the present invention will be further described in detail in conjunction with the accompanying drawings and specific embodiments.

图1为本生产方法工艺流程示意图。Figure 1 is a schematic diagram of the process flow of the production method.

具体实施方式Detailed ways

根据下述实施例,可以更好地理解本发明。实施例所描述的内容仅用于说明本发明,而不应当也不会限制权利要求书中所详细描述的本发明。The present invention can be better understood from the following examples. The content described in the embodiments is only for illustrating the present invention, and shall not and will not limit the present invention described in the claims.

实施例1Example 1

(1)将大豆清洗干净后按照豆水质量比为1∶6的比例将大豆浸泡于水中8小时;(1) Soak the soybeans in water for 8 hours according to the ratio of soybean water mass ratio of 1:6 after cleaning the soybeans;

(2)将浸泡好的大豆和水一起先用粗粉碎机粉碎,然后加入胶体磨中粉碎3次后再用两段式高压均质法均质两次,温度为65℃,第一段均质压强为25MPa,第二段均质压强为4.0MPa;(2) First crush the soaked soybeans and water with a coarse pulverizer, then add them to a colloid mill and pulverize them for 3 times, and then use a two-stage high-pressure homogenization method to homogenize twice at a temperature of 65°C. The mass pressure is 25MPa, and the homogeneous pressure of the second stage is 4.0MPa;

(3)全豆豆乳经85℃灭菌25分钟、冷却至室温后得到灭菌全豆豆乳;(3) Whole soy milk is sterilized at 85°C for 25 minutes and cooled to room temperature to obtain sterilized whole soy milk;

(4)将绿茶、蔗糖和水按照质量比为0.3∶8∶100的比例混合、搅拌,蔗糖充分溶解后加在95℃条件下加热10分钟,冷却,滤掉茶叶,得到糖茶水。(4) Green tea, sucrose and water are mixed and stirred according to the mass ratio of 0.3:8:100. After the sucrose is fully dissolved, the mixture is heated at 95° C. for 10 minutes, cooled, and the tea leaves are filtered to obtain sugar tea water.

(5)将购自市售的红茶菌加入到配好的糖茶水中发酵5天,得到红茶菌发酵液;(5) adding commercially available kombucha into the prepared sugar tea water and fermenting for 5 days to obtain kombucha fermentation liquid;

(6)向灭菌全豆豆乳中加入总质量5%的红茶菌发酵液,于25℃温度下发酵3天得到红茶菌发酵全豆豆乳;(6) Add 5% of the total mass of kombucha fermented liquid to the sterilized whole soymilk, and ferment at 25° C. for 3 days to obtain kombucha fermented whole soymilk;

(7)红茶菌发酵全豆豆乳在4℃温度下后熟5小时后得到红茶菌发酵全豆豆乳成品。(7) Kombucha-fermented whole-soybean soymilk was post-ripened at 4° C. for 5 hours to obtain a finished kombucha-fermented whole-soybean-soybean milk.

如表1结果所示,经红茶菌发酵3天后全豆豆乳中绝大多数人体利用很低的糖苷型大豆异黄酮转化为苷元型大豆异黄酮,而且具有增强肝脏解毒功能的葡萄糖醛酸含量大大增加。说明全豆豆乳经红茶菌发酵后具有更高的保健功能。As shown in the results in Table 1, after 3 days of kombucha fermentation, most of the glycoside-type soy isoflavones in the whole soy milk are converted into aglycon-type soy isoflavones, which are very low in human body utilization, and have glucuronic acid content that enhances liver detoxification function greatly increase. It shows that the whole soy milk has higher health care function after being fermented by kombucha.

表1 发酵前后主要生理活性物质含量变化情况(mg/L)Table 1 Changes of main physiologically active substances before and after fermentation (mg/L)

如表2结果所示,经红茶菌发酵后全豆豆乳中的必需氨基酸含量均大大提高,增加了全豆豆乳的营养价值。As shown in the results in Table 2, the content of essential amino acids in the whole soybean soymilk was greatly increased after being fermented by kombucha, which increased the nutritional value of the whole soybean soymilk.

表2 发酵前后游离氨基酸含量变化情况(mg/100g)Table 2 Changes of free amino acid content before and after fermentation (mg/100g)

注:*代表必需氨基酸.Note: * stands for essential amino acids.

实施例2Example 2

(1)将大豆清洗干净后按照豆水质量比为1∶8的比例将大豆浸泡于水中24小时;(1) Soak the soybeans in water for 24 hours according to the ratio of soybean water mass ratio of 1:8 after cleaning the soybeans;

(2)将浸泡好的大豆和水一起先用粗粉碎机粉碎,然后加入胶体磨中粉碎2次后再用两段式高压均质法均质3次,温度为60℃,第一段均质压强为25MPa,第二段均质压强为5.0MPa;(2) Grind the soaked soybeans and water together with a coarse pulverizer first, then add them to a colloid mill and pulverize them twice, and then use a two-stage high-pressure homogenization method to homogenize three times at a temperature of 60°C. The mass pressure is 25MPa, and the homogeneous pressure of the second stage is 5.0MPa;

(3)全豆豆乳经90℃灭菌20分钟、冷却至室温后得到灭菌全豆豆乳;(3) Whole soy milk is sterilized at 90°C for 20 minutes and cooled to room temperature to obtain sterilized whole soy milk;

(4)将红茶、蔗糖和水按照质量比为0.8∶8∶100的比例混合、搅拌,蔗糖充分溶解后加在95℃条件下加热8分钟,冷却,滤掉茶叶,得到糖茶水。(4) Black tea, sucrose and water are mixed and stirred according to the mass ratio of 0.8:8:100. After the sucrose is fully dissolved, the mixture is heated at 95° C. for 8 minutes, cooled, and the tea leaves are filtered to obtain sugar tea water.

(5)将购自市售的红茶菌加入到配好的糖茶水中发酵15天,得到红茶菌发酵液和菌膜;(5) adding the commercially available kombucha into the prepared sugar tea water and fermenting for 15 days to obtain the kombucha fermented liquid and pellicle;

(6)向灭菌全豆豆乳中加入总质量5%的红茶菌发酵液和5%的菌膜,于28℃温度下发酵2天得到红茶菌发酵全豆豆乳;(6) Adding 5% of the total mass of kombucha fermented liquid and 5% of the bacterial film to the sterilized whole soymilk, and fermenting at 28° C. for 2 days to obtain kombucha fermented whole soymilk;

(7)红茶菌发酵全豆豆乳在4℃温度下后熟4小时后得到红茶菌发酵全豆豆乳成品。(7) Kombucha-fermented whole-soybean soymilk was post-ripened at 4° C. for 4 hours to obtain a finished kombucha-fermented whole-soybean-soybean milk.

实施例3Example 3

(1)将大豆清洗干净后按照豆水质量比为1∶8的比例将大豆浸泡于水中12小时;(1) Soak the soybeans in water for 12 hours according to the ratio of soybean water mass ratio of 1:8 after cleaning the soybeans;

(2)将浸泡好的大豆和水一起先用粗粉碎机粉碎,然后加入胶体磨中粉碎2次后再用两段式高压均质法均质2次,温度为60℃,第一段均质压强为25MPa,第二段均质压强为5.0MPa;(2) Grind the soaked soybeans and water together with a coarse pulverizer first, then add them to a colloid mill and pulverize them twice, and then use a two-stage high-pressure homogenization method to homogenize twice at a temperature of 60°C. The mass pressure is 25MPa, and the homogeneous pressure of the second stage is 5.0MPa;

(3)全豆豆乳经85℃灭菌20分钟、冷却至室温后得到灭菌全豆豆乳;(3) Whole soy milk is sterilized at 85°C for 20 minutes and cooled to room temperature to obtain sterilized whole soy milk;

(4)将红茶、蔗糖和水按照质量比为0.5∶8∶100的比例混合、搅拌,蔗糖充分溶解后加在98℃条件下加热5分钟,冷却,滤掉茶叶,得到糖茶水。(4) Black tea, sucrose and water are mixed and stirred according to the mass ratio of 0.5:8:100, the sucrose is fully dissolved, heated at 98° C. for 5 minutes, cooled, and the tea leaves are filtered to obtain sugar tea.

(5)将葡糖酸醋杆菌(Gluconacetobacter sp.,购自中国工业微生物菌种保藏管理中心,菌种保藏号为CICC 10773)、酿酒酵母(Saccharomyces cerevisiae,菌种购自中国普通微生物菌种保藏管理中心,菌种保藏号为CGMCC 2.3888)、植物乳杆菌(Lactobacillusplantarum,购自中国工业微生物菌种保藏中心,菌种保藏号为CICC 20264)按照菌种数1∶1∶1的比例加入到配好的糖茶水中发酵3天,得到红茶菌发酵液;(5) Gluconacetobacter sp. (Gluconacetobacter sp., purchased from the China Industrial Microorganism Culture Collection Management Center, the strain preservation number is CICC 10773), Saccharomyces cerevisiae (Saccharomyces cerevisiae, the strains were purchased from the China General Microbiology Culture Collection Management Center, strain preservation number is CGMCC 2.3888), Lactobacillus plantarum (Lactobacillus plantarum, purchased from China Industrial Microbiology Culture Collection Center, strain preservation number is CICC 20264) was added to the formula according to the ratio of strain number 1:1:1 The good sugar tea is fermented in water for 3 days to obtain the kombucha fermented liquid;

(6)向灭菌全豆豆乳中加入总质量30%的红茶菌发酵液,于30℃温度下发酵1天得到红茶菌发酵全豆豆乳;(6) Add 30% of the total mass of kombucha fermented liquid to the sterilized whole soymilk, and ferment at 30° C. for 1 day to obtain kombucha fermented whole soymilk;

(7)红茶菌发酵全豆豆乳在2℃温度下后熟8小时后得到红茶菌发酵全豆豆乳成品。(7) Kombucha-fermented whole-soybean soymilk was post-ripened at 2° C. for 8 hours to obtain a finished kombucha-fermented whole-soybean-soybean milk.

实施例4Example 4

(1)将大豆清洗干净后按照豆水质量比为1∶7的比例将大豆浸泡于水中15小时;(1) Soak the soybeans in water for 15 hours according to the ratio of soybean water mass ratio of 1:7 after cleaning the soybeans;

(2)将浸泡好的大豆和水一起先用粗粉碎机粉碎,然后加入胶体磨中粉碎2次后再用两段式高压均质法均质,温度为63℃,第一段均质压强为20MPa,第二段均质压强为4.5MPa;(2) Grind the soaked soybeans and water together with a coarse pulverizer first, then add them to the colloid mill and pulverize them twice, and then use the two-stage high-pressure homogenization method to homogenize. The temperature is 63°C, and the pressure of the first stage is 20MPa, and the homogeneous pressure of the second stage is 4.5MPa;

(3)全豆豆乳经80℃灭菌30分钟、冷却至室温后得到灭菌全豆豆乳;(3) Whole soy milk is sterilized at 80°C for 30 minutes and cooled to room temperature to obtain sterilized whole soy milk;

(4)将绿茶、蔗糖和水按照质量比为0.4∶6∶100的比例混合、搅拌,蔗糖充分溶解后加在100℃条件下加热5分钟,冷却,滤掉茶叶,得到糖茶水。(4) Green tea, sucrose and water are mixed and stirred according to the mass ratio of 0.4:6:100, the sucrose is fully dissolved, heated at 100° C. for 5 minutes, cooled, and the tea leaves are filtered to obtain sugar tea water.

(5)将葡糖酸醋杆菌(Gluconacetobacter sp.,购自中国工业微生物菌种保藏管理中心,菌种保藏号为CICC 10773)、酿酒酵母(Saccharomyces cerevisiae,菌种购自中国普通微生物菌种保藏管理中心,菌种保藏号为CGMCC 2.3888)、植物乳杆菌(Lactobacillusplantarum,购自中国工业微生物菌种保藏中心,菌种保藏号为CICC 20264)按照菌种数3∶1∶1的比例加入到配好的糖茶水中发酵20天,得到红茶菌发酵液和菌膜;(5) Gluconacetobacter sp. (Gluconacetobacter sp., purchased from the China Industrial Microorganism Culture Collection Management Center, the strain preservation number is CICC 10773), Saccharomyces cerevisiae (Saccharomyces cerevisiae, the strains were purchased from the China General Microbiology Culture Collection Management Center, strain preservation number is CGMCC 2.3888), Lactobacillus plantarum (Lactobacillus plantarum, purchased from China Industrial Microorganism Culture Collection Center, strain preservation number is CICC 20264) was added to the formula according to the ratio of strain number 3:1:1 The good sugar tea is fermented in water for 20 days to obtain kombucha fermented liquid and bacterial film;

(6)向灭菌全豆豆乳中加入总质量10%的红茶菌菌膜,于35℃温度下发酵3天得到红茶菌发酵全豆豆乳;(6) Adding 10% of the total mass of kombucha fungus film to the sterilized whole soy milk, fermenting at 35° C. for 3 days to obtain kombucha fermented whole soy milk;

(7)红茶菌发酵全豆豆乳在8℃温度下后熟4小时后得到红茶菌发酵全豆豆乳成品。(7) Kombucha-fermented whole-soybean soymilk was post-ripened at 8° C. for 4 hours to obtain a finished product of kombucha-fermented whole-soybean-soybean milk.

本发明提供了一种红茶菌发酵全豆豆乳及其生产方法的思路及方法,具体实现该技术方案的方法和途径很多,以上所述仅是本发明的优选实施方式,应当指出,对于本技术领域的普通技术人员来说,在不脱离本发明原理的前提下,还可以做出若干改进和润饰,这些改进和润饰也应视为本发明的保护范围。本实施例中未明确的各组成部分均可用现有技术加以实现。The present invention provides a train of thought and method for kombucha fermented whole soy milk and its production method. There are many methods and approaches for realizing the technical solution. The above descriptions are only preferred embodiments of the present invention. Those of ordinary skill in the art can make some improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention. All components that are not specified in this embodiment can be realized by existing technologies.

Claims (7)

  1. Full beans soya-bean milk and its production method 1. a kind of fermented tea ferments, which is characterized in that include the following steps:
    (1) by washing soybean after clean according to beans water quality ratio be 1: 6-8 ratio by soybeans soaking 8-24 hours in water;
    (2) at first with Roughpulverizer, is then added in colloid mill after crushing 2-3 times and uses again High pressure homogenizer homogeneous obtains full beans soya-bean milk 2-3 times;
    (3) full beans soya-bean milk pasteurization, be cooled to room temperature after obtain the full beans soya-bean milk that sterilizes;
    (4) black tea or green tea, sucrose and water are mixed according to the ratio that mass ratio is 0.3-0.8: 5-8: 100, stirring, sucrose fills Divide after dissolving under the conditions of being added in 95-100 DEG C and heats 5-10 minutes, it is cooling, tealeaves is filtered, glucose-tea broth is obtained.
    (5) it (will be prepared purchased from commercially available fermented tea or using acetic acid bacteria, saccharomycete and the fermented tea of the pure bacterium mixed fermentation of lactic acid bacteria Method is with reference to patent of invention:Black-tea fungus drink, Patent No. are produced using pure bacterium mixed fermentation:CN101491362B it) is added to It ferments 3 days or more in the glucose-tea broth prepared, obtains fermented tea fermentation seed;
    (6) fermented tea fermentation seed obtained by step (5) is added obtained by step (3) in the full beans soya-bean milk that sterilizes in suitable under fermentation temperature Fermentation obtains the full beans soya-bean milk of fermented tea fermentation;
    (7) the full beans soya-bean milk of fermented tea fermentation obtains the full beans soya-bean milk finished product of fermented tea fermentation after after-ripening.
  2. Full beans soya-bean milk and its production method 2. a kind of fermented tea according to claim 1 ferments, which is characterized in that step (2) in, described high-pressure homogeneous high-pressure homogeneous for two-part, temperature is 60-65 DEG C, and first segment homogeneous pressure is 25~30MPa, the Two sections of homogeneous pressure are 4.0~4.5MPa.
  3. Full beans soya-bean milk and its production method 3. a kind of fermented tea according to claim 1 ferments, which is characterized in that step (3) in, the pasteurize condition is 80-90 DEG C, 20-30 minutes.
  4. Full beans soya-bean milk and its production method 4. a kind of fermented tea according to claim 1 ferments, which is characterized in that step (5) in, the fermented tea fermentation seed is red tea fungus fermented date liquid or fermented tea mycoderm or red tea fungus fermented date liquid and fermented tea mycoderm The mixture of arbitrary proportion.
  5. Full beans soya-bean milk and its production method 5. a kind of fermented tea according to claim 1 ferments, which is characterized in that step (6) in, the additive amount of fermented tea fermentation seed is the 5-30% of full beans soya-bean milk quality;Fermentation temperature is 25-35 DEG C;Fermentation time It is 1-3 days;Fermentation termination basis for estimation is that the ferment pH of full beans soya-bean milk of fermented tea is 4.2-4.6.
  6. Full beans soya-bean milk and its production method 6. a kind of fermented tea according to claim 1 ferments, which is characterized in that step (7) in, it is 2-6 DEG C that the after-ripening condition, which is temperature, and the time is 4-8 hours
  7. The full beans soya-bean milk 7. the fermented tea that the preparation method described in claim 1~6 any one is prepared ferments.
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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109566758A (en) * 2018-12-04 2019-04-05 苏州金记食品有限公司 A kind of production method of fermented tea Fermentation Function soy protein beverages
CN110115300A (en) * 2019-06-20 2019-08-13 江苏省农业科学院 A kind of fermented tea bean curd and its production method
CN110200078A (en) * 2019-06-25 2019-09-06 吉林农业大学 A kind of preparation method of lipid-loweringing composite probiotics ferment soy yogurt
CN112841537A (en) * 2021-01-28 2021-05-28 吉林省农业科学院 Kangpu tea fermented soymilk and preparation method thereof
CN113068805A (en) * 2021-04-08 2021-07-06 东北农业大学 Fermented soybean milk free of beany flavor and rich in vitamins and preparation method and application thereof
CN114410523A (en) * 2022-01-20 2022-04-29 中国农业科学院茶叶研究所 Strain combination for efficiently preparing black tea fungus and application thereof
CN114468233A (en) * 2022-02-28 2022-05-13 东北农业大学 A kind of fermentation method and application of combining yeast, acetic acid bacteria and lactic acid bacteria for fermenting soybean milk
CN114532490A (en) * 2022-03-01 2022-05-27 东北农业大学 Fermentation method for fermenting soybean milk by combining lactic acid bacteria and black tea fungus and application
CN114568554A (en) * 2020-11-30 2022-06-03 内蒙古伊利实业集团股份有限公司 Fermented tea, preparation method thereof and application thereof in food
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CN116849328A (en) * 2023-07-20 2023-10-10 巴中市产品质量检验检测中心 Compound starter fermented sour soymilk and soybean powder for home use and its preparation method

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103098905A (en) * 2012-12-14 2013-05-15 苏州科谷米业有限公司 Method for making natto milk powder
CN103828928A (en) * 2014-03-21 2014-06-04 王学安 Sour soybean milk drink and making method thereof
CN105145852A (en) * 2015-09-29 2015-12-16 江南大学 Method for improving flavor of bacillus natto fermented whole-soybean milk
CN105707876A (en) * 2016-04-12 2016-06-29 通泰心脑健康管理有限公司 Diet composition with functions of stabilizing and dissolving plaques as well as preparation method and application of diet composition
CN106173721A (en) * 2016-06-30 2016-12-07 东北农业大学 A kind of soy molasses red tea fungus fermented beverage and preparation method thereof
CN106900872A (en) * 2017-03-27 2017-06-30 华南农业大学 A kind of preparation method of the mulberry soya-bean milk containing gamma aminobutyric acid
CN107646988A (en) * 2017-11-08 2018-02-02 广州市妙豆生物科技有限公司 A kind of fermented tea bean curd coagulant and preparation method thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103098905A (en) * 2012-12-14 2013-05-15 苏州科谷米业有限公司 Method for making natto milk powder
CN103828928A (en) * 2014-03-21 2014-06-04 王学安 Sour soybean milk drink and making method thereof
CN105145852A (en) * 2015-09-29 2015-12-16 江南大学 Method for improving flavor of bacillus natto fermented whole-soybean milk
CN105707876A (en) * 2016-04-12 2016-06-29 通泰心脑健康管理有限公司 Diet composition with functions of stabilizing and dissolving plaques as well as preparation method and application of diet composition
CN106173721A (en) * 2016-06-30 2016-12-07 东北农业大学 A kind of soy molasses red tea fungus fermented beverage and preparation method thereof
CN106900872A (en) * 2017-03-27 2017-06-30 华南农业大学 A kind of preparation method of the mulberry soya-bean milk containing gamma aminobutyric acid
CN107646988A (en) * 2017-11-08 2018-02-02 广州市妙豆生物科技有限公司 A kind of fermented tea bean curd coagulant and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
黄友谊: "《茶叶微生物产品学》", 31 August 2017, 中国轻工业出版社 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109566758A (en) * 2018-12-04 2019-04-05 苏州金记食品有限公司 A kind of production method of fermented tea Fermentation Function soy protein beverages
CN110115300A (en) * 2019-06-20 2019-08-13 江苏省农业科学院 A kind of fermented tea bean curd and its production method
CN110200078A (en) * 2019-06-25 2019-09-06 吉林农业大学 A kind of preparation method of lipid-loweringing composite probiotics ferment soy yogurt
CN114568554A (en) * 2020-11-30 2022-06-03 内蒙古伊利实业集团股份有限公司 Fermented tea, preparation method thereof and application thereof in food
CN112841537A (en) * 2021-01-28 2021-05-28 吉林省农业科学院 Kangpu tea fermented soymilk and preparation method thereof
CN113068805A (en) * 2021-04-08 2021-07-06 东北农业大学 Fermented soybean milk free of beany flavor and rich in vitamins and preparation method and application thereof
CN114410523A (en) * 2022-01-20 2022-04-29 中国农业科学院茶叶研究所 Strain combination for efficiently preparing black tea fungus and application thereof
CN114410523B (en) * 2022-01-20 2023-10-31 中国农业科学院茶叶研究所 A strain combination for preparing kombucha and its application
CN114468233A (en) * 2022-02-28 2022-05-13 东北农业大学 A kind of fermentation method and application of combining yeast, acetic acid bacteria and lactic acid bacteria for fermenting soybean milk
CN114532490A (en) * 2022-03-01 2022-05-27 东北农业大学 Fermentation method for fermenting soybean milk by combining lactic acid bacteria and black tea fungus and application
CN116268291A (en) * 2023-02-27 2023-06-23 江苏大学 Preparation and application of a kombucha tofu coagulant
CN116849328A (en) * 2023-07-20 2023-10-10 巴中市产品质量检验检测中心 Compound starter fermented sour soymilk and soybean powder for home use and its preparation method

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Application publication date: 20181113