CN108743401A - A kind of oral cavity root canal sealant and preparation method thereof, application method - Google Patents

Abstract

The invention discloses an oral root canal sealing medicine, a preparation method and a use method thereof. The preparation method of the oral root canal sealing medicine of the present invention is as follows: mixing a cross-linking agent with a glucose chlorhexidine solution, and mixing the obtained mixed solution with chitosan to obtain a hydrogel. Wherein, the mixed solution is obtained by mixing the cross-linking agent of 1.0～30.0mg/mL with 4.0～8.0% glucose chlorhexidine solution according to the volume ratio of 1:1～2:1; the concentration of the chitosan solution 1.0-4.0 mg/mL; the volume ratio of the chitosan solution to the mixed solution is 1:3-1:5. The oral root canal sealing medicine provided by the present invention has better antibacterial and disinfection effects during use, and is very easy to remove after the needle is rinsed, without affecting subsequent root canal filling.

Description

A kind of oral root canal sealing medicine and its preparation method and use method

technical field

The invention relates to a root canal sealing medicine for oral root canal treatment, a preparation method and a use method thereof.

Background technique

Pulpitis and periodontitis are common diseases in stomatology, and the currently recognized treatment for pulpitis and periodontitis is root canal therapy. Mainly, the root canal is preformed, disinfected and cleaned by mechanochemical methods, and the root canal is filled and sealed. Sealing medicine between root canal clinics is a key step in root canal treatment. Its purpose is to completely remove the source of infection in the root canal and prevent re-infection. At present, calcium hydroxide is commonly used as medicine for root canal clinics, but its disinfection and antibacterial effects are not Not ideal, and difficult to remove, affecting subsequent root canal filling. Therefore, it is necessary to find a more effective and thorough root canal disinfection drug.

Contents of the invention

In order to overcome the deficiency of the existing root canal sealing medicine and further improve the effect of the root canal sealing medicine, the present invention utilizes hydrogel-loaded chlorhexidine medicine for disinfection of the root canal sealing medicine between consultations. In vitro experiments show that the antibacterial effect is better (as shown in Figure 2), and can effectively inhibit the growth of Enterococcus faecalis (Enterococcus faecalis is considered to be the main bacteria that causes root canal treatment failure); and needle flushing can quickly remove the root canal seal. The medicine does not affect the follow-up treatment.

The technical solution adopted by the present invention to solve its technical problems is:

An oral root canal sealing medicine is characterized in that it comprises a cross-linking agent, chitosan and chlorhexidine, wherein the cross-linking agent and chitosan are mixed according to the gel-forming ratio, and the chlorhexidine is added into the gel.

Further, the oral root canal sealant is a chlorhexidine hydrogel obtained by mixing and reacting a chitosan solution and a mixed solution; wherein, the mixed solution is obtained by mixing a cross-linking agent and a glucose chlorhexidine solution.

Further, the mixed solution is obtained by mixing the cross-linking agent of 1.0～30.0mg/mL with 4.0～8.0% glucose chlorhexidine solution in a volume ratio of 1:1～2:1; the chitosan solution The concentration is 1.0-4.0 mg/mL; the volume ratio of the chitosan solution to the mixed solution is 1:3-1:5.

Further, the crosslinking agent includes a single component or a mixture of polyethylene glycol di-4-formyl benzoate, oxidized β-cyclodextrin, and polyvinyl alcohol di-4-formyl benzoate; The degree of deacetylation of the polysaccharide is between 85% and 99.9%, the average molecular weight is between 20,000 and 100,000, and the chitosan is soluble chitosan.

A preparation method of an oral root canal sealing medicine, the steps comprising: mixing a cross-linking agent with a glucose chlorhexidine solution, and mixing the obtained mixed solution with chitosan to obtain a hydrogel.

Further, the concentration of the chitosan solution is 3.0 mg/mL, the concentration of the cross-linking agent is 10.0 mg/mL, and the concentration of the dextrose chlorhexidine solution is 6%.

Further, the crosslinking agent includes a single component or a mixture of two 4-formyl benzoic acid polyethylene glycol esters, oxidized β-cyclodextrin, and two 4-formyl benzoic acid polyvinyl alcohol esters; the mixing The solution is mixed and reacted with the chitosan for 2-3 minutes to obtain a hydrogel.

A method for using oral root canal sealing medicine, the steps comprising:

1) For the tooth with pulpitis or periodontitis, it is used to seal the root canal after opening the pulp, uncovering the top of the pulp, extracting the pulp, and preparing the root canal;

2) Pour the root canal sealant into the root canal treated in step 1), and temporarily seal with zinc oxide for a set time;

3) Remove the temporary seal, rinse the root canal, and remove the internal sealant of the root canal.

The oral root canal sealing medicine provided by the present invention is chlorhexidine hydrogel, as long as the ratio of cross-linking agent and chitosan is within the gel-forming range, chlorhexidine can be added arbitrarily, and the ratio is not limited.

The preparation method of chlorhexidine hydrogel is shown in Fig. 1: after cross-linking agent and glucose chlorhexidine solution are mixed, react with chitosan to obtain hydrogel. Specifically: mix 1.0-30.0 mg/mL cross-linking agent with 4.0-8.0% glucose chlorhexidine solution at a ratio of 1:1-2:1; mix chitosan with a concentration of 1.0-4.0 mg/mL The volume ratio of the solution to the above-mentioned mixed solution is 1:3-1:5, and the mixture is reacted at room temperature for 1-5 minutes to obtain the chlorhexidine hydrogel.

Preferably, the degree of deacetylation of the chitosan in the chitosan solution is between 85% and 99.9%, and the average molecular weight is between 20,000 and 100,000, and the chitosan is a soluble chitosan; Hydroxyethyl chitosan.

Preferably, the crosslinking agent includes DF-PEG (polyethylene glycol di-4-formyl benzoate), oxidized β-cyclodextrin, DF-PVA (polyvinyl alcohol di-4-formyl benzoate) ) as a single component or as a mixture.

Preferably, the concentration of the chitosan solution is 3.0 mg/mL, the concentration of the cross-linking agent is 10.0 mg/mL, and the concentration of the glucose chlorhexidine solution is: 6%.

Preferably, the reaction time is 2-3 minutes.

The beneficial effects of the present invention are:

The oral root canal sealing medicine provided by the present invention has better antibacterial and disinfection effects during use, and is very easy to remove after the needle is rinsed, without affecting subsequent root canal filling.

Description of drawings

Fig. 1 is the flow chart of preparation method of chlorhexidine hydrogel of the present invention.

Fig. 2 is the comparison of the antibacterial effects of the present invention and calcium hydroxide after being used for sealing medicine of infected root canals.

Detailed ways

The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

In the invention, the cross-linking agent is mixed with glucose chlorhexidine solution, and then reacted with chitosan to obtain chlorhexidine hydrogel.

Example 1

Get 1 volume concentration of chitosan solution that is 3.0mg/mL, add 3 volume concentrations of 10.0mg/mL DF-PEG and 6% glucose chlorhexidine mixed solution, mix and react at room temperature for 2min to obtain chlorhexidine Hydrogels.

The method of use is as follows:

1. Open the pulp, uncover the pulp roof, pull out the pulp, and use it for root canal sealing after the root canal is prepared.

2. Inject the root canal sealing medicine of the present invention into the root canal with a syringe, temporarily seal with zinc oxide, and seal the medicine for 1 week.

3. Remove the temporary seal, flush the root canal with the syringe needle, and remove the internal sealant of the root canal.

Example 2

Get 1 volume concentration and be the chitosan solution of 3.0mg/mL, add 3 volume concentrations and be the mixed solution of DF-PVA and 6% glucose chlorhexidine of 10.0mg/mL, mix and react 2min at room temperature, obtain chlorhexidine Hydrogels.

The method of use is as follows:

1. Open the pulp, uncover the pulp roof, pull out the pulp, and use it for root canal sealing after the root canal is prepared.

2. Inject the root canal sealing medicine of the present invention into the root canal with a syringe, temporarily seal with zinc oxide, and seal the medicine for 1 week.

3. Remove the temporary seal, flush the root canal with the syringe needle, and remove the internal sealant of the root canal.

Example 3

Take 1 chitosan solution with a volume concentration of 3.0 mg/mL, add 3 volume concentrations of a mixed solution of 10.0 mg/mL oxidized β-cyclodextrin and 6% glucose chlorhexidine, and mix and react at room temperature for 1 min to obtain Chlorhexidine hydrogel.

The method of use is as follows:

1. Open the pulp, uncover the pulp roof, pull out the pulp, and use it for root canal sealing after the root canal is prepared.

2. Inject the root canal sealing medicine of the present invention into the root canal with a syringe, temporarily seal with zinc oxide, and seal the medicine for 1 week.

3. Remove the temporary seal, flush the root canal with the syringe needle, and remove the internal sealant of the root canal.

Example 4

Take 1 volume concentration of ethylene glycol chitosan solution that is 3.0mg/mL, add 4 volume concentrations of 10.0mg/mL DF-PEG and 6% glucose chlorhexidine mixed solution, mix and react at room temperature for 2min, get Chlorhexidine hydrogel.

The method of use is as follows:

1. Open the pulp, uncover the pulp roof, pull out the pulp, and use it for root canal sealing after the root canal is prepared.

2. Inject the root canal sealing medicine of the present invention into the root canal with a syringe, temporarily seal with zinc oxide, and seal the medicine for 1 week.

3. Remove the temporary seal, flush the root canal with the syringe needle, and remove the internal sealant of the root canal.

Example 5

Take 1 volume concentration of ethylene glycol chitosan solution of 3.0mg/mL, add 4 volume concentrations of DF-PVA and 6% glucose chlorhexidine mixed solution of 10.0mg/mL, mix and react at room temperature for 2min, to obtain Chlorhexidine hydrogel.

The method of use is as follows:

1. Open the pulp, uncover the pulp roof, pull out the pulp, and use it for root canal sealing after the root canal is prepared.

2. Inject the root canal sealing medicine of the present invention into the root canal with a syringe, temporarily seal with zinc oxide, and seal the medicine for 1 week.

3. Remove the temporary seal, flush the root canal with the syringe needle, and remove the internal sealant of the root canal.

Example 6

Take 1 volume concentration of ethylene glycol chitosan solution of 3.0 mg/mL, add 3 volume concentration of 10.0 mg/mL oxidized β-cyclodextrin and 6% glucose chlorhexidine mixed solution, mix and react at room temperature After 1 min, chlorhexidine hydrogel was obtained.

The method of use is as follows:

1. Open the pulp, uncover the pulp roof, pull out the pulp, and use it for root canal sealing after the root canal is prepared.

2. Inject the root canal sealing medicine of the present invention into the root canal with a syringe, temporarily seal with zinc oxide, and seal the medicine for 1 week.

3. Remove the temporary seal, flush the root canal with the syringe needle, and remove the internal sealant of the root canal.

The above examples have shown and described the basic principles and preparation methods of the present invention. It should be understood by those skilled in the art that the present invention is not limited by the above-mentioned embodiments. The above-mentioned embodiments and descriptions only illustrate the principle of the present invention, rather than limit the scope of the present invention in any way, without departing from the scope of the present invention. Under the premise, the present invention also has various changes and improvements, and these changes and improvements all fall into the scope of protection.

Claims (10)

1. a kind of oral cavity root canal sealing medicine, it is characterized in that, comprise cross-linking agent, chitosan and chlorhexidine, wherein cross-linking agent and chitosan are mixed to obtain by gelling ratio, and chlorhexidine adds this glue in vivo. 2. oral cavity root canal sealing medicine as claimed in claim 1, is characterized in that, described oral cavity root canal sealing medicine is the chlorhexidine hydrogel that chitosan solution and a mixed solution mixed reaction obtain; Wherein, described The mixed solution is obtained by mixing the cross-linking agent and the glucose chlorhexidine solution. 3. oral root canal sealer as claimed in claim 2, is characterized in that, described mixed solution is by the cross-linking agent of 1.0～30.0mg/mL and 4.0～8.0% glucose chlorhexidine solution according to the volume ratio is 1 : 1-2:1 mixed to obtain; the concentration of the chitosan solution is 1.0-4.0 mg/mL; the volume ratio of the chitosan solution to the mixed solution is 1:3-1:5. 4. The oral root canal sealer according to any one of claims 1 to 3, wherein the crosslinking agent comprises polyethylene glycol di-4-formylbenzoate, oxidized β-cyclodextrin, di A single component or a mixture of 4-formyl polyvinyl benzoate; the degree of deacetylation of the chitosan is between 85% and 99.9%, the average molecular weight is between 20,000 and 100,000, and the chitosan is With soluble chitosan. 5. A preparation method of an oral root canal sealer, the steps comprising: mixing a cross-linking agent with a glucose chlorhexidine solution, and mixing the obtained mixed solution with chitosan to obtain a hydrogel. 6. The method according to claim 5, wherein the mixed solution is 1:1 to 2 by volume of the cross-linking agent of 1.0 to 30.0 mg/mL and 4.0 to 8.0% glucose chlorhexidine solution. : 1 mixed to obtain; the concentration of the chitosan solution is 1.0-4.0 mg/mL; the volume ratio of the chitosan solution to the mixed solution is 1:3-1:5. 7. The method according to claim 5 or 6, wherein the crosslinking agent comprises two 4-formyl benzoic acid polyethylene glycol esters, oxidized β-cyclodextrin, two 4-formyl benzoic acid A single component or a mixture of polyvinyl alcohol ester; the deacetylation degree of the chitosan is between 85% and 99.9%, and the average molecular weight is between 20,000 and 100,000, and the chitosan is a soluble chitosan . 8. method as claimed in claim 7, is characterized in that, the concentration of described chitosan solution is 3.0mg/mL, and the concentration of described linking agent is 10.0mg/mL, and the concentration of described glucose chlorhexidine solution The concentration is 6%. 9. The method of claim 7, wherein the crosslinking agent comprises two 4-formyl benzoic acid polyethylene glycol esters, oxidized β-cyclodextrin, two 4-formyl benzoic acid polyethylene A single component or a mixture of alcohol esters; the mixed solution and the chitosan are mixed and reacted for 2 to 3 minutes to obtain a hydrogel. 10. A method for using oral root canal sealing medicine, the steps comprising: 1) For the tooth with pulpitis or periodontitis, it is used to seal the root canal after opening the pulp, uncovering the top of the pulp, extracting the pulp, and preparing the root canal; 2) Pour the root canal sealant into the root canal treated in step 1), and temporarily seal with zinc oxide for a set time; 3) Remove the temporary seal, rinse the root canal, and remove the internal sealant of the root canal.