CN108719345A - A kind of Herba Origani Vulgaris quintessence oil preparation, preparation method and applications - Google Patents

Abstract

The invention discloses an oregano essential oil preparation, a preparation method and an application thereof. The application of the oregano essential oil in preparing medicines for preventing and controlling Thysanoptera pests on crops; an oregano essential oil preparation, the active ingredient of which is oregano Essential oil, in terms of mass percentage, the content of the oregano essential oil in the preparation is 10% to 20%. The pesticide containing oregano essential oil of the present invention can prevent and treat a variety of thrips, has sufficient raw materials, is easy to process, has low cost, is not easy to produce drug resistance, and can improve the immune system of crops at the same time. It is a kind of pesticide that is safe for humans and animals and has no pollution to the environment Pollution-free plant source thrips control agent.

Description

A kind of oregano essential oil preparation, preparation method and application thereof

technical field

The invention relates to the technical field of pesticides, in particular to an oregano essential oil preparation, a preparation method and an application thereof.

Background technique

Thrips is a general term for insects of the thrips family (Thripida) in the order Thysanoptera. It is small in size, strong in concealment and fast in reproduction. Thrips mainly damage a variety of grains, fruits and vegetables, flowers and gardening. In recent years, the occurrence and spread of thrips have been increasing year by year, and it has become one of the world's important agricultural and forestry pests, causing huge economic losses to agricultural production practices.

At present, the prevention and control of thrips at home and abroad mainly depends on chemical agents, however, the problem of drug resistance is becoming more and more serious, and the pressure on the environment has been increased. Therefore, it is necessary to develop new thrips control agents, and botanical pesticides have become a hot spot in the development and application of new pesticides because of their environmental friendliness, safety to non-target organisms, and resistance to pesticides.

Contents of the invention

The first object of the present invention is to provide the application of oregano essential oil in the preparation of medicines for preventing and controlling Thysanoptera pests on crops, which has a good control effect on such pests.

The second object of the present invention is to provide a preparation of oregano essential oil, which has the characteristics of high efficiency, no residue, safety to non-target organisms, less resistance to drugs, and environmental friendliness.

The third object of the present invention is to provide a preparation method of oregano essential oil, which has easy-to-obtain raw materials, simple processing, low equipment requirements, and high extraction efficiency of active ingredients

The present invention is achieved through the following technical solutions:

According to the first object of the present invention, the oregano essential oil is used for the preparation of a medicine for preventing and controlling Thysanoptera pests on crops.

Optionally, the oregano essential oil is an essential oil product obtained from whole herb of oregano after water extraction and organic solvent distillation.

Optionally, the crops include food, fruits and vegetables, flowers and/or horticultural plants.

Optionally, the Thysanoptera pests include Thrips maize, Thrips western flower and/or Thrips tabaci.

According to the second object of the present invention, a preparation of oregano essential oil, the active ingredient of the preparation is oregano essential oil, and the content of the oregano essential oil in the preparation is 5% to 5% by mass percentage. 30%.

Also, it is an oregano essential oil preparation, the active ingredient of the preparation is oregano essential oil, and the content of the oregano essential oil in the preparation is 10% to 20% in terms of mass percentage.

Optionally, the dosage form of the preparation is selected from thermal mist and soluble liquid.

Optionally, the auxiliary agent used in the hot fog or soluble liquid is at least one of an emulsifier and a solvent;

The emulsifier is selected from one or a mixture of Nongru 0201-B, Nongru 0203-B, Nongru 1601, Nongru 1602 and Nongru 500;

The solvent is selected from one or a mixture of N,N-dimethylformamide, methylnaphthalene, turpentine, soybean oil, dimethyl sulfoxide, methylnaphthalene and absolute ethanol.

According to the third object of the present invention, a preparation method of oregano essential oil preparation comprises: taking 300 g of dried oregano in 400 ml of water and extracting it under ultrasonic waves for 60 min, then distilling for 2 h with a steam distillation device to obtain a distillate, The distillate is distilled with dichloromethane to obtain fractions, and the oregano essential oil is obtained after removing moisture from the fractions, and then the oregano essential oil is prepared into a preparation.

The oregano essential oil preparation of the invention is used for the application of medicines for preventing and controlling Thysanoptera pests on crops.

Compared with the prior art, the present invention has the following advantages:

The pesticide containing oregano essential oil of the present invention has obvious control effect on thrips, is not easy to produce drug resistance, and can play the role of sterilizing and improving the ability of crop immune system at the same time.

Detailed ways

Origanum vulgare (Origanum vulgare L.) is a plant of the Labiatae family. Its whole herb extract contains more than 30 antibacterial compounds. It is a potential natural antibiotic and drug growth promoter. It is of great significance, and after searching relevant literature, it is found that oregano essential oil has a good insecticidal effect, but there is no research report on oregano essential oil in the prevention and treatment of thrips.

The preparation of the oregano essential oil used in the present invention comprises: taking 300g of dried oregano whole herb and putting it into a flask, adding 400ml of water, extracting under ultrasonic waves for 60min, distilling for 2h with a steam distillation device, adding a certain amount of distillate Dichloromethane was distilled with a rotary evaporator, and then filtered after adding an appropriate amount of anhydrous sodium sulfate to absorb water to obtain oregano essential oil. Then, make oregano essential oil into a hot mist or soluble liquid.

The whole herb of oregano was purchased fresh and dried from Zhongxiang Natural Products Company in Ji'an City, Jiangxi Province.

In order to improve the stability of the pesticide containing oregano essential oil, the oregano essential oil is prepared into a hot mist or a soluble liquid for use, and the auxiliary agent used in the hot mist or the soluble liquid is at least one of an emulsifier and a solvent; The emulsifier is selected from one or more of Nongru 0201-B, Nongru 0203-B, Nongru 1601, Nongru 1602 and Nongru 500; the solvent is selected from N,N-dimethylformamide , methyl naphthalene, turpentine oil, soybean oil, dimethyl sulfoxide, methyl naphthalene and absolute ethanol or a mixture of more than one.

The main technical indicators of the soluble liquid of the present invention:

Main technical indicators of the thermal spray agent of the present invention:

The preparation containing oregano essential oil can prevent and treat Thrips maize, Thrips western flower and Thrips tabaci on food, fruits and vegetables, flowers and gardening.

The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

Indoor bioassay research:

Indoor bioassay of oregano essential oil was fumigated in a triangular flask. Before the test, the oregano essential oil was diluted with acetone into different concentration gradients and refrigerated for later use. The specific method is: put 20 healthy and neat test insects into the triangular flask (V=60mL), then fix a filter paper strip (3*1cm) on the rubber stopper, and use a micro-sampler to drip the 1 μL of essential oil solution, quickly tighten the rubber stopper, so that the medicine paper is just suspended at the 1/3 of the center of the bottle, and placed in an intelligent incubator (L:D=16:8, T=25±1°C, RH=60± 10%), after 6 hours, pull out the stopper with filter paper, seal the bottle mouth with plastic wrap, observe the fumigation lethal situation after 24 hours, and use the Abbott formula to calculate the corrected death rate. The death standard of Thrips zea zea is as follows: under a dissecting microscope, touch the insect body lightly with a brush, and its appendages and head and abdomen do not respond. Each treatment was repeated three times, and an acetone control was set.

Corrected mortality rate (%)=(treatment mortality rate−control mortality rate)/(1−control mortality rate)×100%.

Indoor biotest results of oregano essential oil:

Table 1 Laboratory bioassay results of oregano essential oil on thrips

Oregano essential oil is a potential natural antibiotic and drug growth promoter, which is of great significance in poultry feed, feed antibiotics, immunity, etc. Insect effect, the inventor found that oregano essential oil has a good fumigation and killing effect on thrips through indoor bioassay research. From the experimental results in Table 1, it can be known that it has a good effect on various thrips, including thrips maize, Western flower The corrected mortality rates of both thrips and thrips tabaci are more than 96%, and the effect is good.

Based on this, the inventors prepared oregano essential oil into hot mist and soluble liquid respectively for further research.

Example 1: Preparation and application of 10% oregano essential oil hot mist

10% oregano essential oil thermal mist weight percentage: oregano essential oil: 10%, Nongru 0201-B: 5% to 35%, N,N-dimethylformamide: 5% to 35%, turpentine: 5% ~20%, and the rest is made up to 100% by methyl naphthalene.

Example 2: Preparation and application of 15% oregano essential oil hot mist

15% oregano essential oil thermal mist weight percentage: oregano essential oil: 15%, Nongru 0201-B: 5% to 35%, N,N-dimethylformamide: 5% to 35%, soybean oil: 5% % to 20%, and the rest is made up to 100% by methyl naphthalene.

Example 3: Preparation and application of 20% oregano essential oil hot mist

20% oregano essential oil thermal mist weight percentage: oregano essential oil: 20%, Nongru 0203-B: 5% to 35%, dimethyl sulfoxide: 5% to 35%, turpentine oil: 5% to 20%, The rest is 100% from mending to methyl naphthalene.

The field efficacy test of thermal spray adopts plot test:

The plot test was randomly arranged, repeated 3 times, and the area of the plot was 1m ² . 10 pieces of thrips leaves were marked in each plot, the population base of insects was investigated before the medicine, and the number of live thrips was investigated 1, 3, and 7 days after the medicine. Pesticide application is carried out with a hot mist machine. With the help of a hot mist machine, the hot mist of oregano essential oil is pressed into the smoke pipe, mixed with high-temperature and high-speed hot air and sprayed into the atmosphere to form smoke, which diffuses in the field, making the The pesticide enters the body of the pest, thus playing a role in prevention and control. Record the number of insects before and after application of each chemical treatment, calculate the control effect of each chemical treatment, and calculate the corrected control effect.

Calculated as follows:

Control effect = [1-(the number of living insects in the control area before spraying

×Number of live insects after spraying in the treatment area)/(Number of live insects after spraying in the control area

×Number of living insects in the treatment area before spraying)×100%

The test results are as follows:

Table 2 Efficacy test of oregano essential oil thermal mist against thrips

It can be seen from Table 2 that the hot spray of oregano essential oil has a good field control effect on three kinds of thrips, and the control effect period is long, and it has no adverse effects on the target crops within the scope of the test application.

Embodiment 4: Preparation and application of 10% oregano essential oil soluble liquid

10% oregano essential oil soluble liquid by weight percentage: oregano essential oil: 10%, Nongru-1601: 5%-15%, Nongru-500: 1%-5%, and the rest is made up to 100% by absolute ethanol.

Embodiment five: preparation and application of 15% oregano essential oil soluble liquid

15% oregano essential oil soluble liquid by weight percentage: oregano essential oil: 15%, Nongru-1602: 5%-10%, Nongru-500: 1%-5%, and the rest is made up to 100% by absolute ethanol.

Embodiment 6: Preparation and application of 20% oregano essential oil soluble liquid

20% oregano essential oil soluble liquid by weight percentage: oregano essential oil: 20%, Nongru-1602: 5%-15%, Nongru-500: 1%-5%, and the rest is made up to 100% by absolute ethanol.

The test results are as follows:

Soluble liquid formulation field efficacy test adopts plot test:

The plot test was randomly arranged, repeated 3 times, and the area of the plot was 1m ² . 10 pieces of thrips leaves were marked in each plot, the population base of insects was investigated before the medicine, and the number of live thrips was investigated 1, 3, and 7 days after the medicine. The pesticide application uses a sprayer to dilute the pesticide by a corresponding multiple and then apply it, so that the pesticide can enter the body of the pest, so as to play a role in prevention and control. Record the number of insects before and after application of each chemical treatment, calculate the control effect of each chemical treatment, and calculate the corrected control effect.

Calculated as follows:

Control effect = [1-(the number of living insects in the control area before spraying

×Number of live insects after spraying in the treatment area)/(Number of live insects after spraying in the control area

×Number of living insects in the treatment area before spraying)×100%

Table 3 Efficacy test of oregano essential oil soluble liquid in preventing and treating thrips

It can be seen from Table 3 that the soluble liquid preparation of oregano essential oil has good field control effect on three kinds of thrips, and the control effect period is long, and it has no adverse effect on the target crops within the scope of the test drug.

Claims (10)

1. Herba Origani Vulgaris quintessence oil is used to prepare the application of Thysanoptera drug on prevention crops.

2. application according to claim 1, which is characterized in that the Herba Origani Vulgaris quintessence oil be wild marjoram herb through water carry with it is organic The essential oil product of gained after solvent distillation.

3. application according to claim 1, which is characterized in that the crops include grain, fruits and vegetables, flowers and/or Gardening plant.

4. application according to claim 1, which is characterized in that the Thysanoptera includes that maize stays thrips, west Flower thrips and/or onion thrips.

5. a kind of Herba Origani Vulgaris quintessence oil preparation, which is characterized in that the active constituent of the preparation is Herba Origani Vulgaris quintessence oil, by mass percentage Meter, content of the Herba Origani Vulgaris quintessence oil in the preparation are 5%~30%.

6. a kind of Herba Origani Vulgaris quintessence oil preparation, which is characterized in that the active constituent of the preparation is Herba Origani Vulgaris quintessence oil, by mass percentage Meter, content of the Herba Origani Vulgaris quintessence oil in the preparation are 10%~20%.

7. Herba Origani Vulgaris quintessence oil preparation according to claim 5 or 6, which is characterized in that the dosage form of the preparation is selected from thermal fog And soluble liquid.

8. Herba Origani Vulgaris quintessence oil preparation according to claim 7, which is characterized in that make in the thermal fog or soluble liquid Auxiliary agent is at least one of emulsifier and solvent；

The one kind or one kind of emulsifier in agriculture breast 0201-B, agriculture breast 0203-B, agriculture breast 1601, agriculture breast 1602 and agriculture breast 500 Above mixture；

Solvent is selected from N,N-dimethylformamide, methyl naphthalene, turpentine oil, soybean oil, dimethyl sulfoxide (DMSO), methyl naphthalene and absolute ethyl alcohol In one or more kinds of mixtures.

9. a kind of preparation method of Herba Origani Vulgaris quintessence oil preparation, which is characterized in that including：300g is taken to dry wild marjoram grass in 400ml water After extracting 60min under ultrasonic wave, steam distillation device distillation 2h is recycled to obtain distillate, distillate is steamed with dichloromethane It evaporates to obtain fraction, fraction obtains Herba Origani Vulgaris quintessence oil after removing moisture removal, preparation is made in Herba Origani Vulgaris quintessence oil later.

10. the Herba Origani Vulgaris quintessence oil preparation described in the arbitrary claims of claim 5-8 is for preventing Thysanoptera medicine on crops The application of object.