CN108812707B - Thrips killing plant essential oil composition, preparation and application thereof - Google Patents
Thrips killing plant essential oil composition, preparation and application thereof Download PDFInfo
- Publication number
- CN108812707B CN108812707B CN201810650697.5A CN201810650697A CN108812707B CN 108812707 B CN108812707 B CN 108812707B CN 201810650697 A CN201810650697 A CN 201810650697A CN 108812707 B CN108812707 B CN 108812707B
- Authority
- CN
- China
- Prior art keywords
- thrips
- oil
- preparation
- plant essential
- essential oil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 46
- 241001414989 Thysanoptera Species 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 38
- 239000000341 volatile oil Substances 0.000 title claims abstract description 37
- OSWPMRLSEDHDFF-UHFFFAOYSA-N methyl salicylate Chemical compound COC(=O)C1=CC=CC=C1O OSWPMRLSEDHDFF-UHFFFAOYSA-N 0.000 claims abstract description 76
- 239000009637 wintergreen oil Substances 0.000 claims abstract description 71
- 241000196324 Embryophyta Species 0.000 claims abstract description 43
- 239000010661 oregano oil Substances 0.000 claims abstract description 40
- 229940111617 oregano oil Drugs 0.000 claims abstract description 40
- 239000003814 drug Substances 0.000 claims description 19
- 239000004521 hot fogging concentrate Substances 0.000 claims description 12
- 239000004530 micro-emulsion Substances 0.000 claims description 12
- 239000004094 surface-active agent Substances 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 10
- 238000009472 formulation Methods 0.000 claims description 6
- 235000013399 edible fruits Nutrition 0.000 claims description 2
- 235000013311 vegetables Nutrition 0.000 claims description 2
- 241000339374 Thrips tabaci Species 0.000 claims 1
- 230000002265 prevention Effects 0.000 abstract description 13
- 230000002195 synergetic effect Effects 0.000 abstract description 11
- 239000003795 chemical substances by application Substances 0.000 abstract description 10
- 206010059866 Drug resistance Diseases 0.000 abstract description 5
- 210000000987 immune system Anatomy 0.000 abstract description 4
- 244000144972 livestock Species 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 description 33
- 241001529744 Origanum Species 0.000 description 23
- 235000011203 Origanum Nutrition 0.000 description 23
- 239000003921 oil Substances 0.000 description 23
- 230000000694 effects Effects 0.000 description 18
- 239000000575 pesticide Substances 0.000 description 15
- 241000607479 Yersinia pestis Species 0.000 description 11
- 241000238631 Hexapoda Species 0.000 description 10
- 231100000419 toxicity Toxicity 0.000 description 10
- 230000001988 toxicity Effects 0.000 description 10
- 239000004480 active ingredient Substances 0.000 description 8
- 230000001018 virulence Effects 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 229940079593 drug Drugs 0.000 description 6
- 229940051841 polyoxyethylene ether Drugs 0.000 description 6
- 229920000056 polyoxyethylene ether Polymers 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 239000002904 solvent Substances 0.000 description 6
- 241000927584 Frankliniella occidentalis Species 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 5
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 5
- 238000004364 calculation method Methods 0.000 description 5
- 235000009973 maize Nutrition 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 239000012752 auxiliary agent Substances 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 241000894007 species Species 0.000 description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 241000189571 Thrips Species 0.000 description 3
- 241000365763 Thrips setosus Species 0.000 description 3
- 230000001094 effect on targets Effects 0.000 description 3
- 238000013401 experimental design Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000003958 fumigation Methods 0.000 description 3
- 231100000636 lethal dose Toxicity 0.000 description 3
- 229960001047 methyl salicylate Drugs 0.000 description 3
- 231100000989 no adverse effect Toxicity 0.000 description 3
- 231100000820 toxicity test Toxicity 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 241000935799 Lefroyothrips lefroyi Species 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 239000003945 anionic surfactant Substances 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 239000003139 biocide Substances 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002736 nonionic surfactant Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- TVFWYUWNQVRQRG-UHFFFAOYSA-N 2,3,4-tris(2-phenylethenyl)phenol Chemical compound C=1C=CC=CC=1C=CC1=C(C=CC=2C=CC=CC=2)C(O)=CC=C1C=CC1=CC=CC=C1 TVFWYUWNQVRQRG-UHFFFAOYSA-N 0.000 description 1
- DMAXMXPDVWTIRV-UHFFFAOYSA-N 2-(2-phenylethyl)phenol Chemical compound OC1=CC=CC=C1CCC1=CC=CC=C1 DMAXMXPDVWTIRV-UHFFFAOYSA-N 0.000 description 1
- DUIOKRXOKLLURE-UHFFFAOYSA-N 2-octylphenol Chemical compound CCCCCCCCC1=CC=CC=C1O DUIOKRXOKLLURE-UHFFFAOYSA-N 0.000 description 1
- 235000007297 Gaultheria procumbens Nutrition 0.000 description 1
- 235000013628 Lantana involucrata Nutrition 0.000 description 1
- 235000006677 Monarda citriodora ssp. austromontana Nutrition 0.000 description 1
- IGFHQQFPSIBGKE-UHFFFAOYSA-N Nonylphenol Natural products CCCCCCCCCC1=CC=C(O)C=C1 IGFHQQFPSIBGKE-UHFFFAOYSA-N 0.000 description 1
- 240000007673 Origanum vulgare Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 241000736246 Pyrola Species 0.000 description 1
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- OOCMUZJPDXYRFD-UHFFFAOYSA-L calcium;2-dodecylbenzenesulfonate Chemical compound [Ca+2].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O.CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O OOCMUZJPDXYRFD-UHFFFAOYSA-L 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- SNQQPOLDUKLAAF-UHFFFAOYSA-N nonylphenol Chemical compound CCCCCCCCCC1=CC=CC=C1O SNQQPOLDUKLAAF-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000003016 pheromone Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000010773 plant oil Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- -1 polyol fatty acid ester Chemical class 0.000 description 1
- 229920002503 polyoxyethylene-polyoxypropylene Polymers 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001846 repelling effect Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a thrips killing plant essential oil composition, a preparation and application thereof, wherein the composition comprises wintergreen oil and oregano oil, and the weight ratio of the wintergreen oil to the oregano oil is 1: 6-6: 1. the composition can prevent and control thrips, has obvious synergistic effect, is not easy to generate drug resistance, can improve the capability of crop immune system, can obviously improve the vitality of crops, and is a pollution-free botanical thrips prevention and control agent which is safe to human and livestock and has no pollution to the environment.
Description
Technical Field
The invention relates to a preparation method in the technical field of pesticides, in particular to a thrips-killing plant essential oil composition, a preparation and application thereof.
Background
Thrips is a general term for insects of the thrips (thysannoptera) family (Thripida), and is small in size, highly hiding, and fast in reproduction. Up to now, the species of thrips recorded all over the world are more than 2000 species, belonging to about 276 genus in subfamily 4, and 315 species in 79 genus in China. According to incomplete statistics, thrips pests account for about 1% of the recorded species, the phytophagous thrips are taken as the main part, the piercing-sucking mouthparts are used for sucking nutrient substances in tissues and organs to meet the requirements of self growth and development, harm is carried out by directly taking food, laying eggs or spreading viruses, and the loss caused by toxicity transmission is far more than that of direct harm. Thrips is mainly harmful to various grains, fruits, vegetables, flowers, gardening and the like. In recent years, thrips has been increasingly developed year by year, and has become one of the world-wide important agricultural and forestry pests, causing great economic loss to agricultural production practices.
At present, the prevention and treatment of thrips domestically and abroad mainly depend on chemical agents, however, the problem of drug resistance is becoming more serious and the pressure on the environment is increased. The botanical pesticide is a hotspot for research and application of new pesticides due to the advantages of environmental friendliness, safety to non-target organisms, difficulty in generating drug resistance and the like.
Disclosure of Invention
The first purpose of the invention is to provide a thrips-killing plant essential oil composition, the pesticide of wintergreen oil and origanum oil has synergistic effect, the effect of controlling thrips is obvious, the pesticide resistance is not easy to generate, simultaneously, the crop immune system capability can be improved, and the activity can be obviously improved.
The second purpose of the invention is to provide a thrips-killing plant essential oil preparation which has the advantages of high efficiency, no residue, safety to non-target organisms, difficult generation of drug resistance and environmental friendliness.
The third purpose of the invention is to provide the application of the thrips-killing plant essential oil composition.
The invention is realized by the following technical scheme:
according to a first object of the present invention, a thrips-killing plant essential oil composition comprises wintergreen oil and oregano oil.
Optionally, the weight ratio of the wintergreen oil to the oregano oil is 1: 6-6: 1.
optionally, the weight ratio of the wintergreen oil to the oregano oil is 1: 1.
optionally, the weight ratio of the wintergreen oil to the oregano oil is 2: 1.
according to the second object of the present invention, a thrips-killing plant essential oil preparation, the active ingredient of which is the thrips-killing plant essential oil composition of the present invention.
Optionally, the thrips-killing plant essential oil composition accounts for 2-90% of the preparation by mass.
Optionally, the thrips-killing plant essential oil composition accounts for 5-80% of the preparation by mass.
Optionally, the preparation is selected from microemulsion, soluble liquid and hot fogging concentrate, the preparation at least contains a surfactant, and the mass percentage of the surfactant in the preparation is 2-30%.
According to the third object of the invention, the plant essential oil composition is used for preparing the medicine for preventing and treating the thrips of the grain, fruit and vegetable, flowers and/or horticultural plants.
Optionally, the thrips is maize yellow foolish thrips, western flower thrips and/or tobacco thrips.
Compared with the prior art, the invention has the following advantages:
(1) the pesticide containing the wintergreen oil and the origanum oil has a synergistic effect, has an obvious effect of preventing and treating thrips, is not easy to generate drug resistance, can improve the capability of a crop immune system, and can obviously improve the activity of the crop immune system.
(2) The raw material natural resources are extremely rich, the processing is simple, and the requirement on equipment is low.
(3) The main component of the thrips killing agent is derived from natural traditional Chinese medicine plant extracts, so the thrips killing agent has no pollution to the environment, is safe to people and livestock, and is a pollution-free botanical thrips prevention and control agent.
Detailed Description
The wintergreen oil is a plant essential oil extracted from wintergreen leaves, and the main component of the wintergreen oil is methyl salicylate, and the content of the methyl salicylate reaches more than 99%. Methyl salicylate is a pheromone of plants with which plants can be used to combat insect attack. The wintergreen oil has certain effects of bacteriostasis, sterilization, fever reduction, pain relief and the like, has good poisoning and repelling activities on various pests, and has the potential of being developed into an environment-friendly pesticide.
Oregano oil is plant essential oil extracted from whole plant of Oregano, contains more than 30 antibacterial compounds, is a potential natural antibiotic and medicinal growth promoter, and has important significance in livestock and fowl feed, feed antibiotic, immunity, etc. The prevention and control of agricultural diseases, insect pests and weeds by using plant oil is one of the hotspots of the current pesticide research.
The amount of active ingredient in the composition of the present invention depends on the amount of the essential oil applied alone, the compounding ratio of wintergreen oil to oregano oil and the degree of synergy, as well as on thrips. The active ingredient is generally present in the composition in an amount of from 2% to 90% by weight, preferably from 5% to 80% by weight, based on the total weight of the composition. The range of active ingredient content varies from formulation type to formulation type.
The preparation of the invention at least contains one kind of surfactant and solvent, and the preparation can also contain stabilizer, anti-freezing agent and the like which are well known by the technical personnel according to different preparation forms, and the rest is supplemented by water.
The content of the surfactant is 2-30% of the total weight of the preparation, and at least one of nonionic surfactant or anionic surfactant can be adopted. The nonionic surfactant can be at least one of styrol polyoxyethylene polyoxypropylene ether, tristyryl phenol polyoxyethylene ether, octyl phenol polyoxyethylene ether, nonylphenol polyoxyethylene ether, castor oil ethylene oxide adduct and derivatives thereof, polyol fatty acid ester and ethylene oxide adduct thereof, fatty alcohol polyoxyethylene ether, etc. The anionic surfactant is at least one of calcium dodecylbenzene sulfonate, alkylphenol polyoxyethylene ether phosphate, phenethyl phenol polyoxyethylene ether phosphate, etc. The solvent can be at least one of ethanol, isopropanol, n-butanol, ethyl acetate, acetone, etc. The water may be one of distilled water, deionized water and tap water, and is more preferably deionized water.
For example, when the microemulsion is prepared, the thrips-killing plant essential oil composition is 5-40 percent, the surfactant is 5-50 percent, the auxiliary agent is 5-35 percent and the deionized water is added to 100 percent according to the mass percentage.
When the composition is prepared into a soluble liquid, the thrips killing plant essential oil composition accounts for 5-45 percent, the surfactant accounts for 5-10 percent and the solvent accounts for 5-90 percent.
When the composition is prepared into the hot fogging concentrate, the thrips killing plant essential oil composition accounts for 10-40%, the surfactant accounts for 5-35%, the solvent accounts for 5-35%, and the auxiliary agent accounts for 5-20%.
The microemulsion of the invention has the main technical indexes that:
the main technical indexes of the soluble liquid of the invention are as follows:
the hot fogging concentrate of the invention has the main technical indexes that:
and (3) synergistic research:
1. the research method comprises the following steps:
the invention adopts a method combining indoor toxicity measurement and field test. Firstly, determining the co-toxicity coefficient (CTC) of two medicaments compounded according to a certain proportion by indoor toxicity measurement, wherein the CTC is less than 80 and is antagonistic action, the CTC is more than or equal to 80 and is less than or equal to 120 and is additive action, and the CTC is more than 120 and is synergistic action, and then carrying out field test on the basis.
The test method comprises the following steps: the fumigation activity is measured by a triangular flask fumigation method. The specific method comprises the following steps: 20 healthy and tidy test insects are inoculated into a triangular flask (V is 60mL), then a filter paper strip (3 x 1cm) is fixed on a rubber plug, 1 mu L of essential oil solution is dripped on the filter paper strip by using a micro-sample injector, the rubber plug is quickly plugged, the paper strip is just hung at 1/3 in the center of the flask, the flask is placed in an intelligent incubator (L: D is 16: 8, T is 25 +/-1 ℃, RH is 60 +/-10%), after 6h, the triangular flask mouth is opened, the plug with the filter paper is pulled off, the flask mouth is sealed by a preservative film, the fumigation death condition is observed after 24h, each treatment is repeated three times, and acetone control is set. If the control mortality is greater than 10%, the test is considered invalid and the corrected mortality is calculated using the Abbott equation.
The calculation formula is as follows:
mortality (%). mortality vs. total number of insects × 100%
Corrected mortality (%) - (treatment mortality-control mortality)/(1-control mortality) × 100%
Calculating toxicity regression equation by using probability value analysis method and calculating LC50And (4) calculating the cotoxicity coefficient CTC according to a Sun cloud Peel formula method.
The calculation formula is as follows (taking wintergreen oil as a standard medicament, the virulence index is 100):
virulence index (TI) of active ingredient B-LC 50 of wintergreen oil/LC 50X 100 of active ingredient B
Virulence index (ATI) of M-LC 50/M LC 50X 100 of wintergreen oil
The theoretical virulence index (TTI) of M is TI XP wintergreen oil of wintergreen oil + TI XP active B of active B
TTI x 100 of ATI/M with co-toxicity coefficient (CTC) ═ M
In the formula: m is a mixture with different proportions; b is oregano oil; p active ingredient B is the proportion of the active ingredient B in the composition; p wintergreen oil is the proportion of wintergreen oil in the composition;
2. the research results are as follows:
2.1 synergism study of maize yellow foolish thrips
The test agents are provided by Zhongxiang natural plants, Inc. in Jian city, Jiangxi province.
And (3) experimental design: the effective lethal concentration range of the wintergreen oil, the origanum oil and the mixture of the wintergreen oil and the origanum oil in different proportions is determined through a preparation test. Virulence assay results were as follows:
TABLE 1 toxicity test result analysis table of combined wintergreen oil and origanum oil for Microthrips zeae
As can be seen from Table 1, the proportion of the wintergreen oil and the origanum oil for preventing and treating maize yellow foolish thrips is 1: 6-6: when the toxicity coefficient is 1, the co-toxicity coefficients are all larger than 120, which indicates that the toxicity coefficients are 1: 6-6: the synergistic effect is shown in the range of 1, when the ratio of the wintergreen oil to the origanum oil is 1:1, the co-toxicity coefficient is the largest, and the synergistic effect is most obvious.
2.2 synergy study of frankliniella occidentalis:
the test agents are provided by Zhongxiang natural plants, Inc. in Jian city, Jiangxi province.
And (3) experimental design: the effective lethal concentration range of the wintergreen oil, the origanum oil and the mixture of the wintergreen oil and the origanum oil in different proportions is determined through a preparation test. Results of virulence determination
TABLE 2 toxicity test result analysis table of combined wintergreen oil and origanum oil for Frankliniella occidentalis
| Reagent for testing | Proportioning | LC50 | Co-toxicity coefficient (CTC) |
| Wintergreen oil | - | 3.02 | - |
| Oregano oil | - | 3.78 | - |
| Wintergreen oil: oregano oil | 1:6 | 3.10 | 121.23 |
| Wintergreen oil: oregano oil | 1:5 | 3.26 | 130.50 |
| Wintergreen oil: oregano oil | 1:4 | 2.69 | 133.79 |
| Wintergreen oil: oregano oil | 1:3 | 2.46 | 146.35 |
| Wintergreen oil: oregano oil | 1:2 | 2.86 | 147.78 |
| Wintergreen oil: oregano oil | 1:1 | 2.34 | 152.62 |
| Wintergreen oil: oregano oil | 2:1 | 3.34 | 161.04 |
| Wintergreen oil: oregano oil | 3:1 | 2.19 | 145.19 |
| Wintergreen oil: oregano oil | 4:1 | 3.55 | 135.05 |
| Wintergreen oil: oregano oil | 5:1 | 3.43 | 127.54 |
| Wintergreen oil: oregano oil | 6:1 | 3.67 | 124.87 |
As can be seen from Table 2, the ratio of wintergreen oil to origanum oil for preventing and treating frankliniella occidentalis is 1: 6-6: when the toxicity coefficient is 1, the co-toxicity coefficients are all larger than 120, which indicates that the toxicity coefficients are 1: 6-6: the synergistic effect is shown in the range of 1, when the ratio of the wintergreen oil to the origanum oil is 2:1, the co-toxicity coefficient is the largest, and the synergistic effect is most obvious.
2.3 tobacco thrips synergy research:
the test agents are provided by Zhongxiang natural plants, Inc. in Jian city, Jiangxi province.
And (3) experimental design: the effective lethal concentration range of the wintergreen oil, the origanum oil and the mixture of the wintergreen oil and the origanum oil in different proportions is determined through a preparation test. Results of virulence determination
TABLE 3 toxicity test result analysis table for tobacco thrips by compounding wintergreen oil and origanum oil
| Reagent for testing | Proportioning | LC50 | Co-toxicity coefficient (CTC) |
| Wintergreen oil | - | 3.34 | - |
| Oregano oil | - | 3.97 | - |
| Wintergreen oil: oregano oil | 1:6 | 3.18 | 121.5682 |
| Wintergreen oil: oregano oil | 1:5 | 3.01 | 127.8749 |
| Wintergreen oil: oregano oil | 1:4 | 2.77 | 132.8372 |
| Wintergreen oil: oregano oil | 1:3 | 2.98 | 127.2233 |
| Wintergreen oil: oregano oil | 1:2 | 2.29 | 163.1084 |
| Wintergreen oil: oregano oil | 1:1 | 2.15 | 168.7382 |
| Wintergreen oil: oregano oil | 2:1 | 2.35 | 150.0662 |
| Wintergreen oil: oregano oil | 3:1 | 3.11 | 144.9162 |
| Wintergreen oil: oregano oil | 4:1 | 2.44 | 120.1912 |
| Wintergreen oil: oregano oil | 5:1 | 2.65 | 129.462 |
| Wintergreen oil: oregano oil | 6:1 | 2.36 | 123.3747 |
As can be seen from Table 3, the ratio of wintergreen oil to origanum oil for preventing and treating frankliniella occidentalis is 1: 6-6: when the toxicity coefficient is 1, the co-toxicity coefficients are all larger than 120, which indicates that the toxicity coefficients are 1: 6-6: the synergistic effect is shown in the range of 1, when the ratio of the wintergreen oil to the origanum oil is 1:1, the co-toxicity coefficient is the largest, and the synergistic effect is most obvious.
The following examples are given for the detailed implementation and specific operation of the present invention, but the scope of the present invention is not limited to the following examples.
Example 1: microemulsion preparation
(1) Preparation of a preparation: when the plant essential oil composition is prepared into microemulsion, the components and the weight percentage are as follows: 5-40% of plant essential oil composition, 5-50% of surfactant, 5-35% of auxiliary agent and deionized water added to 100%. The specific formulation is shown in Table 4.
TABLE 4 preparation of microemulsions containing wintergreen oil and oregano oil
(2) The microemulsion thrips prevention and treatment efficacy test: the field drug effect test adopts a cell test, the cell test is randomly arranged and repeated for 3 times, and the cell area is 1m2. The number of thrips leaves is 10 pieces per cell, the population base number is investigated before the drug is taken, and the number of thrips is investigated 1, 3 and 7 days after the drug is taken. And recording the insect quantity before and after the treatment and application of each medicament, and calculating the control effect of each medicament treatment. And calculating the correction control effect.
The calculation formula is as follows:
the control effect is [1- (number of live insects before drug application in control area x number of live insects after drug application in treatment area)/(number of live insects after drug application in control area x number of live insects before drug application in treatment area x 100%
The experiment is arranged in a test field of northwest agriculture and forestry science and technology university, the test medicament is provided by Zhongxiang natural plant Co., Ltd in Jian city, Jiangxi province, and the control medicaments are wintergreen oil microemulsion and origanum oil microemulsion which are self-prepared. The indexes of the yellow and slow-release thrips zeae pests are investigated before the pesticides are applied, the pesticides are applied at the initial stage of the pest generation, the indexes of the pests are investigated 1 day, 3 days and 7 days after the pesticides are applied, and the control effect is calculated.
The test results are shown below:
TABLE 5 microemulsion thrips prevention and treatment efficacy test
As can be seen from the table 5, the microemulsion compounded by the wintergreen oil and the origanum oil has good control effect on three thrips, the control effect is superior to that of a single agent, and the control period is long. Has no adverse effect on target crops in the application range of the test.
Example 2: soluble liquid agent
(1) Preparation of a preparation: when the plant essential oil composition is prepared into a soluble liquid, the components and the weight percentage are as follows: 5-45% of plant essential oil composition, 5-10% of surfactant and 5-90% of solvent. The specific formulation is shown in Table 6.
TABLE 6 preparation of soluble solutions containing wintergreen oil and oregano oil
(2) The efficacy test of the thrips prevention and cure: the experiment is arranged in a test field of northwest agriculture and forestry science and technology university, the test medicament is provided by Zhongxiang natural plant Co., Ltd in Jian city, Jiangxi province, and the control medicament is a wintergreen oil soluble liquid and an origanum oil soluble liquid which are both self-prepared. The indexes of the yellow thrips tabaci pests in the maize are investigated before the pesticide is applied, the indexes of the pests are investigated 1 day, 3 days and 7 days after the pesticide is applied, and the prevention effect is calculated (the calculation method is the same as the example 1).
The test results are shown below:
TABLE 7 test of the efficacy of soluble liquid for controlling thrips
As can be seen from Table 7, the wintergreen oil and origanum oil compounded soluble liquid has good control effect on three thrips, the control effect is superior to that of a single agent, and the control period is longer. Has no adverse effect on target crops in the application range of the test.
Example 3: hot fogging concentrate
(1) Preparation of a preparation: when the plant essential oil composition is prepared into the hot fogging concentrate, the components and the weight percentage are as follows: 10-40% of plant essential oil composition, 5-35% of surfactant, 5-35% of solvent and 5-20% of auxiliary agent. The specific formulations are shown in Table 8.
TABLE 8 preparation of hot fogging concentrate containing wintergreen oil and oregano oil
(2) The drug effect test of the hot fogging concentrate for preventing and treating thrips: the experiment is arranged in a test field of northwest agriculture and forestry science and technology university, the test medicament is provided by Zhongxiang natural plant Co., Ltd in Jian city, Jiangxi province, and the control medicaments are wintergreen oil hot fogging concentrate and origanum oil hot fogging concentrate which are self-prepared. The indexes of the yellow thrips tabaci pests in the maize are investigated before the pesticide is applied, the indexes of the pests are investigated 1 day, 3 days and 7 days after the pesticide is applied, and the prevention effect is calculated (the calculation method is the same as the example 1).
The test results are shown below:
TABLE 9 test of efficacy of hot fogging concentrate on prevention and treatment of thrips
As can be seen from Table 9, the wintergreen oil and origanum oil compounded hot fogging concentrate has good prevention effect on three thrips, the prevention and treatment effect is superior to that of a single agent, and the prevention and treatment period is long. Has no adverse effect on target crops in the application range of the test.
Claims (9)
1. A thrips-killing plant essential oil composition, which comprises wintergreen oil and oregano oil;
the weight ratio of the wintergreen oil to the oregano oil is 1: 6-4: 1.
2. a thrips-killing plant essential oil composition according to claim 1, wherein the weight ratio of said wintergreen oil to oregano oil is 1: 1.
3. a thrips-killing plant essential oil composition according to claim 1, wherein the weight ratio of said wintergreen oil to oregano oil is 2: 1.
4. a thrips-killing plant essential oil preparation, characterized in that the effective component of the preparation is the thrips-killing plant essential oil composition as claimed in any one of claims 1-3.
5. The thrips-killing plant essential oil preparation as claimed in claim 4, wherein the thrips-killing plant essential oil composition is contained in the preparation in an amount of 2-90% by mass.
6. The thrips-killing plant essential oil preparation as claimed in claim 4, wherein the thrips-killing plant essential oil composition is contained in the preparation in an amount of 5-80% by weight.
7. The thrips-killing plant essential oil preparation according to claim 4, wherein the formulation is selected from the group consisting of microemulsion, soluble liquid and hot fogging concentrate, the preparation at least contains surfactant, and the surfactant accounts for 2-30% of the preparation by weight.
8. Use of the thrips-killing plant essential oil composition as claimed in any one of claims 1-3 for preparing a medicament for controlling thrips in foodstuffs, fruits, vegetables and flower plants.
9. The use according to claim 8, wherein said thrips is thrips zeae or thrips tabaci.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711449833.6 | 2017-12-27 | ||
| CN2017114498336 | 2017-12-27 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108812707A CN108812707A (en) | 2018-11-16 |
| CN108812707B true CN108812707B (en) | 2021-06-08 |
Family
ID=64094919
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810319827.7A Withdrawn CN108782615A (en) | 2017-12-27 | 2017-12-27 | One kind killing thrips plant essential oil composition, preparation and its application |
| CN201810650697.5A Active CN108812707B (en) | 2017-12-27 | 2018-06-22 | Thrips killing plant essential oil composition, preparation and application thereof |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810319827.7A Withdrawn CN108782615A (en) | 2017-12-27 | 2017-12-27 | One kind killing thrips plant essential oil composition, preparation and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN108782615A (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113367134B (en) * | 2021-05-13 | 2022-07-26 | 中国热带农业科学院环境与植物保护研究所 | Pesticide composition for preventing and treating banana thrips |
| CN114287450B (en) * | 2022-01-13 | 2023-02-28 | 南宁市农业科学研究所 | Pesticide composition for preventing and treating sugarcane thrips |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102014616A (en) * | 2008-03-19 | 2011-04-13 | 蒂拉德克公司 | Pest control using natural pest control agent blends |
| JP2012036097A (en) * | 2010-08-03 | 2012-02-23 | Sumitomo Chemical Co Ltd | Pest-controlling composition |
| WO2013050967A1 (en) * | 2011-10-04 | 2013-04-11 | 0903608 B.C. Ltd. | Pest control formulations and methods of making and using same |
| CN104041504A (en) * | 2014-05-08 | 2014-09-17 | 中国农业科学院茶叶研究所 | Behavior control agent for control tea tree and leaf empoasca vitis and dendrothrips minowai priesner |
-
2017
- 2017-12-27 CN CN201810319827.7A patent/CN108782615A/en not_active Withdrawn
-
2018
- 2018-06-22 CN CN201810650697.5A patent/CN108812707B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102014616A (en) * | 2008-03-19 | 2011-04-13 | 蒂拉德克公司 | Pest control using natural pest control agent blends |
| JP2012036097A (en) * | 2010-08-03 | 2012-02-23 | Sumitomo Chemical Co Ltd | Pest-controlling composition |
| WO2013050967A1 (en) * | 2011-10-04 | 2013-04-11 | 0903608 B.C. Ltd. | Pest control formulations and methods of making and using same |
| CN104041504A (en) * | 2014-05-08 | 2014-09-17 | 中国农业科学院茶叶研究所 | Behavior control agent for control tea tree and leaf empoasca vitis and dendrothrips minowai priesner |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108812707A (en) | 2018-11-16 |
| CN108782615A (en) | 2018-11-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103081912B (en) | Celastrus angulatus and cnidium lactone compounded pesticide and preparation method thereof | |
| CN108184835B (en) | Acaricide and its application | |
| CN101176460B (en) | Insecticidal composition with synergistic effect containing flubenzuron and abamectin | |
| CN103141488B (en) | Insecticide compounded with azadirachtin and osthole and preparation method thereof | |
| CN104322572B (en) | A kind of plant insecticide | |
| CN106508991A (en) | Biological pesticide and preparation method thereof | |
| CN104381255A (en) | Reagent used for inducing frankliniella occidentalis | |
| CN108812707B (en) | Thrips killing plant essential oil composition, preparation and application thereof | |
| CN107494080A (en) | A kind of method that coccid is killed in the potted plant cultivating process of succulent | |
| CN105284917B (en) | A kind of plant pesticide composition containing nimbin and caryophyllus oil | |
| CN101803605B (en) | Insecticidal composition of butene-fipronil and spinosad | |
| CN101849556A (en) | A kind of botanical synergistic compound acaricide and preparation method thereof | |
| CN103636621A (en) | Pesticide composition containing etoxazole and azadirachtin | |
| CN102657216A (en) | Use of pesticide mixed preparation containing chlorpyrifos and high-efficiency cypermethrin in control of Athetis lepigone Moschler | |
| CN111011367A (en) | Plant source bait for killing solenopsis invicta and preparation method thereof | |
| CN102524292A (en) | Special effect formula for controlling whitefly | |
| CN113475529B (en) | Nematode preparation for preventing and treating flower slugs and preparation method and application thereof | |
| CN107821413A (en) | A kind of cockroach bait formulation and preparation method thereof | |
| CN103875714A (en) | Bacillus thuringiensis.spinosyns.trisiloxane polyoxyethylene ether composition and use of bacillus thuringiensis.spinosyns.trisiloxane polyoxyethylene ether composition for preventing and controlling rice boring insects | |
| CN108402061B (en) | Biopesticide composition for preventing and treating tea geometrid | |
| CN115474608B (en) | Composition for controlling Wuta-tsai diseases and application thereof | |
| CN110050799A (en) | A kind of biological prevention and control agent containing wood vinegar and its application, preparation and application | |
| CN103918705A (en) | Pesticidal composition containing spirotetramat and lambda-cyhalothrin and application thereof | |
| CN104413082A (en) | Insect-expelling agent special for planting elausena lansium | |
| CN109907051B (en) | Insecticidal composition containing fluazinam and propargite |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20210203 Address after: 712100 Shaanxi Province, Xi'an city Yangling District Tai Road No. 3 demonstration Applicant after: NORTHWEST A & F University Address before: 712100 Shaanxi Province, Xi'an city Yangling District Tai Road No. 3 demonstration Applicant before: RESEARCH & DEVELOPMENT CENTER OF BIORATIONAL PESTICIDE YANGLING NONGKEDA |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |