CN108679448B - Microfluidic flow online adjustment device and detection method - Google Patents
Microfluidic flow online adjustment device and detection method Download PDFInfo
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- CN108679448B CN108679448B CN201810559797.7A CN201810559797A CN108679448B CN 108679448 B CN108679448 B CN 108679448B CN 201810559797 A CN201810559797 A CN 201810559797A CN 108679448 B CN108679448 B CN 108679448B
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Abstract
本发明公开了一种微流体流量在线调节装置与检测方法，所述装置包括供气气源、减压阀、比例压力阀、第一液体容器、阀门、第一压力传感器、第二压力传感器、微流体通道、微流控芯片、第二液体容器、称重仪和微处理器。减压阀与供气气源、比例压力阀的气体入口连通；比例压力阀与第一液体容器连通；第一液体容器与阀门连通；阀门与微流体通道连通；微流体通道与微流控芯片连通；微流控芯片与第二液体容器连通；第一压力传感器布置于微流体通道的入口，第二压力传感器布置于微流体通道的出口；第二液体容器放置于称重仪上；压力传感器与微处理器相连；微处理器与比例压力阀相连。本发明可实现微流体流量在线的稳定调节与精确测量，具有微流体流量调节稳定、检测速度快、检测精度高等优点，为促进微流控系统在交叉学科领域的应用研究，提供技术支持。
The invention discloses a microfluidic flow online adjustment device and a detection method. The device comprises an air supply air source, a pressure reducing valve, a proportional pressure valve, a first liquid container, a valve, a first pressure sensor, a second pressure sensor, Microfluidic channel, microfluidic chip, second liquid container, weighing instrument and microprocessor. The pressure reducing valve is communicated with the gas supply air source and the gas inlet of the proportional pressure valve; the proportional pressure valve is communicated with the first liquid container; the first liquid container is communicated with the valve; the valve is communicated with the microfluidic channel; the microfluidic channel is communicated with the microfluidic chip communication; the microfluidic chip is communicated with the second liquid container; the first pressure sensor is arranged at the inlet of the microfluidic channel, and the second pressure sensor is arranged at the outlet of the microfluidic channel; the second liquid container is placed on the weighing instrument; the pressure sensor Connected to the microprocessor; the microprocessor is connected to the proportional pressure valve. The invention can realize the stable regulation and accurate measurement of the microfluidic flow online, has the advantages of stable microfluidic flow regulation, fast detection speed, high detection accuracy, and provides technical support for promoting the application research of the microfluidic system in the interdisciplinary field.
Description
Technical Field
The invention relates to an online micro-fluid flow adjusting device and a detection method, belonging to the field of micro-fluid.
Background
Syringe pumps are common flow regulating elements for microfluidic systems. The injection pump is driven by a stepping motor, the diameter of the injector is selected, the rotating speed of the stepping motor is set, and an ideal value of the output flow of the injection pump can be calculated. However, the use of syringe pumps to regulate microfluidic flow in microfluidic systems has a number of disadvantages, such as: the actual output flow has periodic pulsation, the flow cannot be detected on line, the flow regulation speed is slow, the precision is low and the like. With the wide application of the microfluidic system in the fields of chemistry, biology, medicine and the like, the pressure-driven microfluidic flow regulating device can replace an injection pump to realize stable flow regulation of the microfluidic system. The pressure driving device is adopted to adjust the flow of the microfluid, so that the periodic flow pulsation caused by a pump source is avoided, the flow adjustment is stable, and the speed is high. However, as the driving pressure increases, there is a nonlinear characteristic of the microfluidic flow rate, and the ideal output flow rate of the pressure driving device is difficult to calculate accurately. Particularly, the pressure driving device and the liquid drop micro-fluidic system are integrated, and the volume of the liquid drop is directly related to the flow rate of the micro-fluid, so that how to detect and adjust the flow rate of the micro-fluid on line in the liquid drop forming process is very critical for improving the stability of liquid drop formation and the control precision of the volume of the liquid drop and promoting the development of the liquid drop micro-fluidic system in the interdisciplinary field.
Disclosure of Invention
The invention provides a microfluid flow online adjusting device and a detection method, which are integrated with a microfluid system, can realize online stable adjustment and accurate measurement of microfluid flow, and provide technical support for promoting the application research of the microfluid system in the cross subject field.
The purpose of the invention is realized by the following technical scheme:
the invention relates to a microfluid flow online adjusting device and a detection method, which comprises an air supply source, a pressure reducing valve, a proportional pressure valve, a first liquid container, a valve, a first pressure sensor, a second pressure sensor, a microfluid channel, a microfluidic chip, a second liquid container, a weighing instrument and a microprocessor. Wherein:
the gas inlet of the pressure reducing valve is communicated with the compressed air outlet of the gas supply source, and the gas outlet of the pressure reducing valve is communicated with the gas inlet of the proportional pressure valve;
the gas outlet of the proportional pressure valve is communicated with the gas inlet of the first liquid container, and the liquid outlet of the first liquid container is communicated with the inlet of the valve;
the outlet of the valve is communicated with the inlet of the microfluidic channel, and the outlet of the microfluidic channel is communicated with the inlet of the microfluidic chip;
the outlet of the microfluidic chip is communicated with the inlet of the second liquid container;
the first pressure sensor is arranged at the inlet of the microfluidic channel, the second pressure sensor is arranged at the outlet of the microfluidic channel, and the second liquid container is placed on the weighing instrument;
the pressure signal output ends of the first pressure sensor and the second pressure sensor are connected with the pressure signal input end of the microprocessor;
and the control command signal output end of the microprocessor is connected with the control command signal input end of the proportional pressure valve.
A microfluid flow online detection method using the device comprises the following steps:
firstly, a gas supply source enters a first liquid container filled with liquid through a pressure reducing valve and a proportional pressure valve, and a certain gas driving pressure is formed in the first liquid container;
secondly, as the driving pressure of the gas is increased, the liquid in the first liquid container flows into the microfluidic channel through the valve under the driving of the gas pressure, the inlet pressure and the outlet pressure of the microfluidic channel are respectively measured by the first pressure sensor and the second pressure sensor, the pressure difference at two ends of the microfluidic channel is obtained, and the theoretical flow of the microfluidic is calculated;
and thirdly, the microprocessor collects pressure signals of the first pressure sensor and the second pressure sensor in real time, outputs a control instruction signal to the proportional pressure valve, changes the gas control pressure of the proportional pressure valve, adjusts the microfluid flow on line, and measures the change rule of the liquid mass in the second liquid container along with time by the weighing instrument to obtain the actual flow of the microfluid, thereby realizing the on-line detection of the microfluid flow.
Compared with the prior art, the invention has the following advantages:
1) the micro-fluid flow is stably regulated, the actual output flow has no periodic flow pulsation, and the pressure driving device is adopted to regulate the micro-fluid flow, so that the dynamic regulation speed of the flow can be improved, and the regulation range of the flow is enlarged.
2) The online detection precision of the microfluid flow is high, and the pressure sensor has high measurement precision, and the microfluid flow and the pressure difference at two ends of the microfluid channel have good linear characteristics, so that the online accurate detection of the microfluid flow can be realized by measuring the pressure difference at two ends of the microfluid channel.
3) The on-line detection speed of the microfluid flow is high, and the on-line rapid detection of the microfluid flow can be realized by measuring the change of the pressure difference at two ends of a microfluid channel when the microfluid flow is changed due to the high response speed of the pressure sensor.
4) The micro-fluid flow on-line adjustment has high precision, the pressure difference at two ends of the micro-fluid channel is measured by the pressure sensor and fed back to the microprocessor, the microprocessor outputs a control signal to change the gas control pressure of the proportional pressure valve, and the on-line precise adjustment of the micro-fluid flow can be realized.
Drawings
FIG. 1 is a schematic diagram of the operation of the microfluidic flow online regulating device and the detection method of the present invention;
FIG. 2 is a schematic diagram of a circular tube of PTFE according to an embodiment;
FIG. 3 is a control block diagram of the on-line regulation of three microfluidic flows according to an embodiment.
Detailed Description
The first embodiment is as follows: the following describes the present embodiment with reference to fig. 1 and fig. 2, and the microfluidic flow online adjustment device and the detection method according to the present embodiment are composed of an air supply source 1, a pressure reducing valve 2, a proportional pressure valve 3, a first liquid container 4, a valve 5, a first pressure sensor 6, a second pressure sensor 7, a microfluidic channel 8, a microfluidic chip 9, a second liquid container 10, a weighing instrument 11, and a microprocessor 12. Wherein:
the gas inlet of the pressure reducing valve 2 is communicated with the compressed air outlet of the gas supply source 1, and the gas outlet of the pressure reducing valve 2 is communicated with the gas inlet of the proportional pressure valve 3;
the gas outlet of the proportional pressure valve 3 is communicated with the gas inlet of the first liquid container 4, and the liquid outlet of the first liquid container 4 is communicated with the inlet of the valve 5;
the outlet of the valve 5 is communicated with the inlet of the microfluidic channel 8, and the outlet of the microfluidic channel 8 is communicated with the inlet of the microfluidic chip 9;
the outlet of the microfluidic chip 9 is communicated with the inlet of the second liquid container 10;
the first pressure sensor 6 is arranged at the inlet of the microfluidic channel 8, the second pressure sensor 7 is arranged at the outlet of the microfluidic channel 8, and the second liquid container 10 is placed on a weighing instrument 12;
the pressure signal output ends of the first pressure sensor 6 and the second pressure sensor 7 are connected with the pressure signal input end of the microprocessor 12;
and the control command signal output end of the microprocessor 12 is connected with the control command signal input end of the proportional pressure valve 3.
The first pressure sensor 6 and the second pressure sensor 7 respectively measure the inlet pressure and the outlet pressure of the microfluidic channel 8, the pressure difference at two ends of the microfluidic channel 8 is obtained and fed back to the microprocessor 12, the microprocessor 12 outputs a control signal, the gas control pressure of the proportional pressure valve 3 is changed, and the online accurate adjustment of the microfluidic flow is realized.
The working principle is as follows:
in the embodiment, compressed air is supplied by an air supply source, the compressed air enters a first liquid container filled with liquid through a pressure reducing valve and a proportional pressure valve, a certain air driving pressure is formed in the first liquid container, the liquid in the first liquid container flows into a microfluidic channel through a valve under the driving of air pressure along with the increase of the air driving pressure, the air driving pressure of the first liquid container is controlled by the proportional pressure valve, and the flow of the liquid flowing into the microfluidic channel is adjusted by changing the air driving pressure. The first pressure sensor and the second pressure sensor are arranged at two ends of the microfluidic channel, respectively measure the inlet pressure and the outlet pressure of the microfluidic channel, simultaneously feed back the pressure difference at the two ends of the microfluidic channel to the microprocessor, calculate the theoretical flow of the microfluidic and compare the theoretical flow with the set flow of the microfluidic based on the linear relation between the microfluidic flow and the pressure difference at the two ends of the microfluidic channel, and output a control signal by the microprocessor to change the gas control pressure of the proportional pressure valve, thereby realizing the online accurate regulation of the microfluidic flow. Meanwhile, the change rule of the mass of the liquid in the second liquid container along with time is measured by the weighing instrument, so that the actual flow of the microfluid is obtained, and the online accurate detection of the microfluid flow is realized.
The implementation mode can realize the online stable regulation and accurate detection of the micro-fluid flow, and provides technical support for promoting the application research of the micro-fluid system in the interdisciplinary field. The air supply pressure of the air supply source 1 is 100-200 kPa, the pressure adjusting range of the proportional pressure valve 3 is 0.1-100 kPa, the maximum measuring range of the first pressure sensor 6 and the second pressure sensor 7 is 100 kPa, the measuring precision reaches 0.05kPa, the maximum measuring range of the weighing instrument is 500 g, and the minimum resolution is 0.0001 g. Microfluidic flow rateQIs regulated byThe range is 0.002 mL/min-120 mL/min.
The second embodiment is as follows: this embodiment mode will be described below with reference to fig. 2, and the first embodiment mode will be further described. To ensure that the microfluidic flow rate has a linear relationship with the pressure difference across the microfluidic channel 8 (microfluidic flow rate)Q= proportionality coefficientKX pressure differenceΔP) The online accurate detection of the microfluid flow is realized, the microfluid channel 8 adopts a PTFE round tube structure, and the length of the round tube is requiredLAnd inner diameterdThe ratio of the ratio is more than 20:1, and the Reynolds number Re of the micro-fluid flow is satisfied in the circular tube<1.0, the microfluidics is in laminar flow regime.
In the present embodiment, the inner diameter of the PTFE circular tubedThe variation range of (1) is 0.1-1.0 mm, and the length of the PTFE round tubeLThe variation range of (a) is 10.0-40.0 mm according to the flow rate of the microfluidQTo obtain the pressure difference between two ends of the microfluidic channelΔPThe variation range of (A) is 0.2 to 35.0 kPa. The online detection precision of the microfluid flow reaches 0.1%.
The third concrete implementation mode: this embodiment mode will be described below with reference to fig. 3, and the first embodiment mode will be further described. In order to realize the online stable adjustment of the micro-fluid flow, the first pressure sensor 6 and the second pressure sensor 7 respectively measure the inlet pressure and the outlet pressure of the micro-fluid channel 8, the pressure difference at two ends of the micro-fluid channel 8 is obtained and fed back to the microprocessor 12, the microprocessor 12 outputs a control signal, the gas control pressure of the proportional pressure valve 3 is changed, and the micro-fluid flow is adjusted online.
In the embodiment, the online adjustment time of the micro-fluid flow is less than 0.5 s, and the online adjustment precision of the micro-fluid flow reaches 0.1%.
Claims (4)
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| CN110508339A (en) * | 2019-08-30 | 2019-11-29 | 上海理工大学 | A kind of microfluid sampling device of air pressure driving |
| CN112051251B (en) * | 2020-09-09 | 2024-02-09 | 北京北分瑞利分析仪器(集团)有限责任公司 | Gas circuit control system of atomic fluorescence spectrometer |
| CN112934282A (en) * | 2021-03-26 | 2021-06-11 | 西北大学 | Self-feedback high-flux microfluidic system and method |
| CN113280262A (en) * | 2021-05-08 | 2021-08-20 | 中山市鸿盛生物科技有限公司 | Chemical raw material measurable supplement system |
| CN113960216A (en) * | 2021-11-10 | 2022-01-21 | 北京莱伯泰科仪器股份有限公司 | Microfluid quantitative adding device |
| CN114415746A (en) * | 2021-11-29 | 2022-04-29 | 中国船舶工业集团公司第七0八研究所 | A novel cascaded microfluidic intelligent control system |
| CN114460325A (en) * | 2022-01-21 | 2022-05-10 | 苏州莱博睿思生物科技有限公司 | A liquid micro-flow control system and method and a micro-fluidic detection device |
| CN115489141A (en) * | 2022-09-23 | 2022-12-20 | 中国航空制造技术研究院 | Injection pipeline system of composite material forming mold and injection method thereof |
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| US4188968A (en) * | 1977-10-28 | 1980-02-19 | Johnson Controls, Inc. | Flow system with pressure level responsive air admission control |
| CN101750194B (en) * | 2010-01-18 | 2012-07-04 | 北京艾迪西暖通科技有限公司 | Test platform for comprehensive performance of pressure-reducing valve |
| CN102840995B (en) * | 2012-09-10 | 2015-03-25 | 临海市伟达汽车部件有限公司 | Performance test analysis system for EGR (Exhaust Gas Recirculation) cooler |
| CN103091095B (en) * | 2013-01-11 | 2015-08-19 | 浙江大学台州研究院 | Pneumatic proportional pressure valve test unit |
| CN103775700A (en) * | 2014-03-03 | 2014-05-07 | 北京航空航天大学 | Pressure regulating valve |
| CN104696706B (en) * | 2015-01-20 | 2017-08-04 | 哈尔滨工业大学 | Microfluidic Flow Regulator Based on Pneumatic Drive |
| CN105278587A (en) * | 2015-10-26 | 2016-01-27 | 哈尔滨工业大学 | Multi-channel microfluidic pressure source |
| CN207297311U (en) * | 2017-09-07 | 2018-05-01 | 北京长城华冠汽车科技股份有限公司 | Test device and testboard bay |
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