CN108651457A - Application of the isoamyl alcohol in luring mythimna separata - Google Patents

Abstract

The invention relates to the application of isoamyl alcohol in luring armyworms. The invention discloses a kind of armyworm attractant belonging to the technical field of insect attractant preparation. The active ingredient of the attractant is isoamyl alcohol, phytol or phytyl 2-methylbutyrate. The armyworm attractant of the invention has the characteristics of high efficiency in trace amounts, low cost, non-toxic to humans and animals, environmental friendliness, resistance to pests and the like.

Description

Application of Isoamyl Alcohol in Attracting Armyworm

technical field

The invention relates to the application of isoamyl alcohol in attracting armyworms and belongs to the technical field of preparation of insect attractants.

Background technique

The armyworm Mythimna separate Walker belongs to Lepidoptera: Noctuidae. It is a typical long-distance migratory pest and an important pest that seriously threatens the safety of food production in my country.

According to the regulation effect of plant volatiles on the behavior of armyworm, the discovery and preparation of armyworm attractants are of great significance for improving the comprehensive management level of armyworm, the quality of agricultural products and reducing the loss of insect pests in food production.

Contents of the invention

The object of the present invention is to provide the application of isoamyl alcohol in luring armyworms. The isoamyl alcohol is used for the prevention and control and control of the armyworm, and reduces the pest loss of target crops.

The structural formula of described isoamyl alcohol is:

The armyworm is preferably a female armyworm, and the luring effect is more remarkable.

The use concentration of the isoamyl alcohol is 0.01-0.6 mg/m ² ; preferably 0.25-0.4 mg/m ² . The placement position is suitable for a height of 60-120 cm from the ground, preferably 100 cm.

The present invention also provides an attractant for armyworms, comprising: using isoamyl alcohol as an active component alone, or using isoamyl alcohol and phytol as active components, or using isoamyl alcohol, 2-methyl The active ingredient is phyllyl butyrate, or isoamyl alcohol, phytol, and 2-methyl phytyl butyrate are used as active ingredients.

The structural formula of the phytol is:

The structural formula of the 2-methyl butyrate phyllyl ester is:

Specifically, the mass percentage of the active ingredient in the attractant is 40-100%.

When the active ingredients of the attractant are isoamyl alcohol and phytol, the mass ratio of isoamyl alcohol to phytol is 1-3:1-3, preferably 1:1.

When the active ingredient of the attractant is isoamyl alcohol and 2-methyl butyric acid phyllyl ester, the mass ratio of isoamyl alcohol to 2-methyl butyric acid phyllyl ester is 1-3:1-3, preferably 1 :1.

When the active ingredient of the attractant is isoamyl alcohol, phytol, and 2-methyl phytyl butyrate, the mass ratio of isoamyl alcohol, phytol, and 2-methyl phytyl butyrate is 4- 8:1-6:1-6, preferably 2:1:1.

Besides the active components, the attractant also includes a solvent and a carrier, the solvent is selected from dichloromethane, and can also be replaced by n-hexane.

As one of the embodiments of the present invention, the attractant includes: an active component and a carrier; the carrier can be selected from various types of rubber material carriers known by those skilled in the art. Specifically, each of the rubber material carriers contains 10-40 mg of the active component as the lure.

The preparation of the attractant can be prepared by the conventional attractant preparation methods mastered by those skilled in the art.

The present invention also provides a method for preparing an attractant for armyworms, comprising:

(1) adding the active component into the carrier to prepare the lure;

(2) adding a solvent to the carrier for the first time to dissolve the active component;

(3) After the solvent is volatilized, add the solvent for the second time, so that the active component can fully penetrate into the carrier;

(4) After the solvent is volatilized again, put the lure core into the insect trapping device.

Wherein, the active ingredient is selected from isoamyl alcohol, or isoamyl alcohol and phytol, or isoamyl alcohol and 2-methyl butyrate phytol, or isoamyl alcohol and phytol, 2-methylbutanol Folate.

Wherein, the carrier is a rubber material carrier, and the content of isoamyl alcohol in the carrier is 10-40 mg.

Wherein, the solvent is selected from dichloromethane or n-hexane, and the amount added each time is preferably 50-150 μl.

The use mode of the attractant is: the concentration is 0.01-0.6 mg/m ² , preferably 0.25-0.4 mg/m ² . The placement position of the lure is suitable for 60-120cm from the ground, preferably 100cm.

The present invention also provides the application of the above-mentioned attractant in the preparation of armyworm attractant drugs.

The beneficial effects of the present invention are as follows:

The present invention uses plant volatiles such as isoamyl alcohol, phytol and phytol 2-methyl butyrate as active ingredients for attracting armyworms. The above active ingredients have a strong luring effect, and the method for preparing the attractant by using the above active ingredients is simple , the conditions are feasible, and it has the characteristics of high efficiency, low cost, non-toxic to humans and animals, environmental friendliness, and not easy to produce resistance.

Detailed ways

The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

The preparation of embodiment 1 armyworm attractant lure core

The standard sample of isoamyl alcohol, phytol and 2-methyl phytyl butyrate is added to the rubber carrier (each rubber carrier contains 10-40mg of drug), and the lure is prepared, and then 50-150ul of dichloromethane is added to the Dissolve the compound in the rubber carrier, and then add 50-150ul of clean dichloromethane after the solvent has evaporated, so that the compound components can fully penetrate into the rubber. After the solvent is volatilized again, the lure core is put into the device for placing the lure core in the insect trap.

Effect verification 1 Outdoor lure experiment on armyworm

Time and place: From August 27, 2015 to September 14, 2013, the experiment of attracting migratory armyworms was conducted at the Plant Protection Postgraduate Experimental Base of the Chinese Academy of Agricultural Sciences in Beihuangcheng Township, Changdao County, Yantai City, Shandong Province.

The test was carried out on the playground at the base.

Specific method: Place a high-altitude searchlight insect trap light in the center of the playground, put the lure core prepared in Example 1 into the insect trap, and set 3 repetitions for each treatment, arrange them randomly, and simultaneously use infiltrated clean dichloromethane The rubber carrier was used as a control. Set up an insect trap every 5-15m. The lure core of the insect trap is 100cm above the ground. After setting, investigate the number of armyworms attracted, the ratio of males and females, the progress of ovarian development and the number of mating times of female moths every two days. The results are shown in Table 1.

Attractive effect of table 1 compound on armyworm

The test results show that the average trapping amount of the active ingredient isoamyl alcohol of the present invention to armyworms is obviously higher than that of phytol, 2-methyl butyric acid phytol and the control. The development progress of the ovaries of the female moths is all first grade, and none of them have mated, which shows that the active ingredient isoamyl alcohol of the present invention has a very strong attracting activity on the newly emerged armyworm adults.

Effect Verification 2 Determination of Attractive Activity against Armyworm

Using 1-2 day-old armyworm adults (bred indoors) as test insects, a Y-type olfactometer was used to test the attracting activity of the compound isoamyl alcohol of the present invention and active compositions with different ratios. Wherein, the contrast is clean dichloromethane.

The compound to be tested isoamyl alcohol was formulated into a 0.1mol/L test drug solution. The air flow rate of the Y-shaped olfactometer was set at 0.4 L/min. Release a single armyworm adult into the base arm of the Y-shaped tube for observation, and start timing when the armyworm adult is at 1/2 of the length of the Y-shaped tube base arm. When the armyworm adults crossed the left or right arm 3cm and stayed in this area for more than 10s, it was recorded as a selection response; when there was no obvious selection tendency after putting the armyworm adults in for 5 minutes, it was recorded as no response.

59-65 armyworms were tested for each treatment, and the reaction rate of armyworm and the selectivity rate of the compound to be tested were calculated. The formula is as follows, and the test results are shown in Table 2.

Response rate (%)=100×(test armyworm total number-non-responsive armyworm number)/test armyworm total number

Selectivity (%)=100×select the armyworm number/test armyworm total number of compound to be tested=100 * the active composition of compound/different proportioning is to the attracting activity of armyworm

The results in Table 2 show that the compound isoamyl alcohol of the present invention and active compositions of different proportions such as isoamyl alcohol, 1:1 isoamyl alcohol and phytol and 1:1 isoamyl alcohol and 2-methylbutyric acid Leaf alcohol esters have very strong attractant activity to armyworms that have just emerged, and the selection rates of armyworms are all above 60%. Therefore, using the active ingredients of the present invention to make an armyworm attractant to recruit and kill armyworms will be of great significance to improving the level of prevention and control of armyworms, the quality of agricultural products and reducing the loss of insect pests in food production.

Although the present invention has been described in detail with general descriptions and specific embodiments above, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, the modifications or improvements made on the basis of not departing from the spirit of the present invention all belong to the protection scope of the present invention.

Claims (10)

1. application of the isoamyl alcohol in luring mythimna separata.

2. application according to claim 1, which is characterized in that the mythimna separata is female mythimna separata.

3. application according to claim 1 or 2, which is characterized in that a concentration of 0.01-0.6mg/ of use of the isoamyl alcohol m², preferably 0.25-0.4mg/m²。

4. according to any applications of claim 1-3, which is characterized in that the isoamyl alcohol placement location is apart from ground height 60-120cm。

5. a kind of attractant for mythimna separata, which is characterized in that include active constituent, the active constituent is：

It is individually for active component with isoamyl alcohol；

Alternatively, using isoamyl alcohol, phytol as active component；

Alternatively, using isoamyl alcohol, 2-Methyl Butyric Acid leaf-alcohol ester as active component；

Or using isoamyl alcohol, phytol, 2-Methyl Butyric Acid leaf-alcohol ester as active component.

6. attractant according to claim 5, which is characterized in that the mass percent of active constituent is in the attractant 40-100%.

7. attractant according to claim 5 or 6, which is characterized in that the active constituent of the attractant is isoamyl alcohol, leaf When green alcohol, the mass ratio of isoamyl alcohol and phytol is 1-3:1-3, preferably 1:1；

When the active constituent of the attractant is isoamyl alcohol, 2-Methyl Butyric Acid leaf-alcohol ester, isoamyl alcohol and 2-Methyl Butyric Acid leaf-alcohol ester Mass ratio be 1-3:1-3, preferably 1:1；

When the active constituent of the attractant is isoamyl alcohol, phytol, 2-Methyl Butyric Acid leaf-alcohol ester, isoamyl alcohol, phytol, 2- first The mass ratio of base butyric acid leaf-alcohol ester is 4-8:1-6:1-6, preferably 2:1:1.

8. according to any attractants of claim 5-7, which is characterized in that the attractant also includes solvent, carrier.

9. attractant according to claim 5, which is characterized in that the attractant includes：Active component, carrier；It is described Carrier is rubber material carrier；Contain 10-40mg active components in each rubber material carrier.

10. a kind of preparation method of attractant for mythimna separata, which is characterized in that including：

(1) active component is added in carrier, prepares lure；

(2) solvent is added for the first time into carrier, dissolves active component；

(3) after waiting for solvent volatilization, second of addition solvent makes active component be fully infiltrated into carrier；

(4) after waiting for that solvent volatilizees again, lure is put into insect trap device.