CN108627583A - The detection method of royal jelly ingredient the amount of migration in a kind of filter stick - Google Patents

Abstract

The present invention relates to a kind of detection methods of royal jelly ingredient the amount of migration in filter stick, include the following steps：Step（1）, puff number determine；Step（2）, according to puff number set needed for heating chamber temperature；Step（3）, suction；Step（4）, constituent analysis.The present invention is eluted using purified air stream, eliminate the complicated smoke components background interference generated during practical cigarette burning, the precision and accuracy of measurement result significantly improve, and avoid the complex sample purification during conventional special flavoring filter stick ingredient is detected to flue gas migration analysis and enrichment procedure.The configuration of the present invention is simple, the situation of oral cavity suction temperatures cannot really be reflected by avoiding conventional temperature unanimously, reduce the influence of complicated flue gas.

Description

A detection method for the migration of royal jelly components in filter sticks

technical field

The invention relates to a method for detecting the migration of royal jelly components, in particular to a method for detecting the migration of royal jelly components in filter sticks, and belongs to the field of physical and chemical testing of tobacco auxiliary materials.

Background technique

At present, the commonly used special flavoring filter sticks include multi-component compound flavoring filter, pop-bead filter, flavoring sheet filter, flavoring thread filter, particle flavoring filter, molded paper flavoring filter, triacetin flavoring filter etc. Royal jelly is a pulpy substance secreted by the hypopharyngeal and upper glands of worker bees after eating pollen and nectar, and is exclusively eaten by bee larvae and queen bees. Royal jelly contains proteins, amino acids, vitamins, acetylcholine and organic acids and other nutrients. It is a natural health food suitable for human beings and has various medical and health functions. At present, coffee royal jelly is widely used in special flavoring filters such as multi-component compound flavoring filters, pop-bead filters, flavoring slice filters, and granular flavoring filters.

At present, the test method for the migration of ingredients added to special flavoring filter sticks into cigarette smoke usually uses a smoking machine to suck cigarettes, and then capture the smoke components for analysis. Due to the very complex background generated when the cigarette is burned, it will seriously interfere with the determination of the migration components of the special flavoring filter rod, the determination of its components is very difficult, and the error of the analysis results is also very large. How to truly and accurately analyze and detect the safety components of special flavoring filter sticks has become the focus of tobacco analysis research.

Contents of the invention

In order to solve the above problems, the object of the present invention is to provide a method for detecting the migration of royal jelly components in filter sticks. Concrete scheme of the present invention is as follows:

A method for detecting the migration of royal jelly components in a filter stick, comprising the steps of:

Step (1), determine the number of suction ports

Insert a temperature measuring probe into the starting part of the far lip end of the cigarette to be tested that contains the flavoring filter, smoke on a conventional smoking machine, and measure the starting position of the flavoring filter through the temperature measuring probe when each puff is smoked the temperature of the smoke and determine the number of puffs for the whole cigarette;

Step (2), set the temperature of the required heating chamber according to the number of suction ports

Set the air temperature in several heating chambers of the detection device according to the actual smoking temperature of the cigarette, and set the number of suction ports;

Step (3), suction

Connect the smoking machine, the smoke trap and the filter holder in sequence, remove the filter tip of the special filter cigarette from the cigarette, install it on the filter holder, start the smoking machine and smoke according to the standard conditions, The air sucked comes from heating chambers of different temperatures, which are switched sequentially according to the number of suction ports. The airflow temperature of each suction through the filter tip is consistent with the actual airflow temperature of the cigarette smoking machine; the simulated smoke is tested with methanol-acetonitrile solution. Flue gas elution and capture;

Step (4), component analysis;

Perform ultra-high performance liquid chromatography analysis on the captured liquid, analysis conditions:

Symmetry 300TM C18, 4.6mm×250mm, 5μm;

The mobile phase is acetone: 1% acetic acid solution; 0-3min is 40:60, v/v; 5-10min is 70:30, v/v, gradient elution; flow rate: 1.5 mL/min; column temperature 40°C, Injection volume: 10 μL.

Further, a detection device is used for detection. The detection device includes several heating chambers. One end of the heating chamber is connected to the air inlet, and the other end is connected to the air outlet pipe. The air outlet pipe is arranged around the turntable. As the turntable rotates, one end of the first air intake pipe communicates with one of the air outlet pipes at a certain moment, the other end of the first air intake pipe communicates with one end of the second air intake pipe, the second air intake pipe is fixed, and the other end is connected to the filter holder. The filter holder is connected with the smoke trap, and the smoke trap is connected with the smoking machine.

Further, there are 10 heating chambers, each of which is equipped with a temperature regulating device, and the heating temperature range is 30-200°C.

Further, in step (4), 200ml of methanol-acetonitrile solution is used to elute and trap the simulated flue gas, wherein the volume ratio of methanol-acetonitrile is 1:1.

Compared with the prior art, the present invention has the following beneficial effects:

(1) The present invention adopts the purified air flow for elution, which eliminates the background interference of complex smoke components generated during the actual cigarette burning process, and significantly improves the precision and accuracy of the measurement results.

(2) Simple headspace or solvent extraction can be used for direct analysis, which makes the determination of royal jelly components very easy, and avoids the complicated sample purification and enrichment in the analysis and detection of conventional special flavored filter rod components to flue gas migration analysis. set operation.

(3) The present invention is simple in structure and easy to operate. The detection device of the present invention is provided with several heating chambers, and different temperatures are set, so that the suction temperature of the human oral cavity can be very good, avoiding that the conventional temperature cannot truly reflect the suction temperature of the oral cavity situation, reducing the impact of complex smoke.

(4), the analysis method of the present invention is simple, fast, accurate, high sensitivity, low detection limit, effective and feasible, easy to operate, accurate and sensitive, and the test period is shortened by 10-15 minutes compared with the existing detection method, which has the advantages of popularization and application value.

Description of drawings

Fig. 1 is the structural representation of detection device of the present invention;

In Figure 1: I is the turntable; II is the filter holder; III is the smoke trap; IV is the smoking machine; V is the air inlet;

1-the first communication port, 1'-the first heating chamber; 2-the second communication port, 2'-the second heating chamber; 3-the third communication port, 3'-the third heating chamber; 4-the fourth communication 4'-the fourth heating chamber; 5-the fifth communication port, 5'-the fifth heating chamber; 6-the sixth communication port, 6'-the sixth heating chamber; 7-the seventh communication port, 7'- Seventh heating chamber; 8-eighth communication port, 8'-eighth heating chamber; 9-ninth communication port, 9'-ninth heating chamber; 10-tenth communication port, 10'-tenth heating chamber; 11-the first air intake pipe; 12-the second air intake pipe.

Detailed ways

The present invention will be described in further detail below in conjunction with the accompanying drawings and examples, but the present invention is not limited in any way, and any changes or improvements based on the teachings of the present invention fall within the protection scope of the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The materials or equipment used are not indicated by the manufacturer, and they are all conventional products that can be obtained through purchase.

Example 1

The detection device of this embodiment includes a first heating chamber 1', a second heating chamber 2', a third heating chamber 3', a fourth heating chamber 4', a fifth heating chamber 5', a sixth heating chamber 6', The seventh heating chamber 7', the eighth heating chamber 8', the ninth heating chamber 9', and the tenth heating chamber 10', one end of each heating chamber is connected to the air inlet, and the other end is connected to the air outlet pipe, and the air outlet pipe surrounds the turntable 1 Set to form the first communication port 1, the second communication port 2, the third communication port 3, the fourth communication port 4, the fifth communication port 5, the sixth communication port 6, the seventh communication port 7, The eighth communication port 8, the ninth communication port 9, the tenth communication port 10, the first air intake pipe 11 is included on the turntable 1, and the first air intake pipe 11 rotates with the turntable 1, and one end of the first air intake pipe 11 connects with it at a certain moment. One communication port communicates, the other end of the first air intake pipe 11 communicates with one end of the second air intake pipe 12, the second air intake pipe 12 is fixed, the other end of the second air intake pipe 12 is connected with the filter holder II, and the filter holder II Connect the smoke trap III, the smoke trap III is connected to the smoking machine IV, an air intake pipe can be arranged at one end of the air inlet, and one end of the air intake pipe is connected with the air inlet V.

The heating chamber is equipped with a temperature regulating device, and the heating temperature range is 30-200°C. There is an electronic heating component on the heating chamber, which can quickly heat the air in the chamber to the specified temperature and control the temperature precisely.

The turntable and the smoking machine are connected with the control system, and the control system controls the automatic rotation and switching of the turntable, and is consistent with the cigarette smoking process of the smoking machine, that is, when the first puff is taken, the first communication port communicates with the first intake pipe 11; When the second port is used, switch to the second communication port so that the second communication port communicates with the first air intake pipe 11; When switching, the outside air will not directly enter the first air intake pipe, and the air in the entire suction process comes from each heating chamber, which highly simulates human smoking.

The filter rod holder is a filter tip holder with adjustable circumference, and its circumference can be adjusted to meet all the circumference specifications of commercially available cigarettes. The smoke trap is a Cambridge filter. The gas paths between all components are metal or glass heat-insulated pipelines.

The detection method of the royal jelly component migration amount used for the special flavoring filter stick of the present embodiment is carried out as follows using the above-mentioned detection device:

Insert a temperature-measuring probe at the far lip end of the cigarette with a flavored composite filter of A brand, and insert a temperature-measuring probe into it, smoke it on an ordinary smoking machine, and use the temperature-measuring probe to measure the amount of time passed during each puff. The temperature of the smoke at the beginning of the incense filter, and determine the number of puffs in the whole cigarette.

According to the measured suction temperature and the number of suction ports, set the air temperature in the air heating chamber in the detection device, among which, heating chamber 1' (first port 36.5 ℃), heating chamber 2' (second port 37.9 ℃) , heating chamber 3' (third port 41.2 ℃), heating chamber 4' (fourth port 43.8 ℃), heating chamber 5' (fifth port 45.1 ℃), heating chamber 6' (sixth port 46.7 ℃), heating chamber Chamber 7' (7th port 48.4 ℃), heating chamber 8' (eighth port 50.3 ℃). Set the number of suction ports to 8 in total.

Remove the filter tip of the brand A flavored compound filter cigarette from the cigarette, install it on the filter holder, start the smoking machine to smoke according to the standard conditions (ISO smoking mode), and when you take the first puff, The hot air in the first heating chamber passes through the filter stick; then it is switched by the turntable, when the second port is sucked, the hot air in the second heating chamber passes through the filter rod, and so on, the number of ports is switched in turn. The air flow through the filter simulates the smoke flow when the cigarette is smoked, and elutes the components of the flavoring compound filter.

Use 200ml of methanol-acetonitrile (1:1) solution to carry out flue gas elution and trapping on the simulated flue gas. The collected solution was subjected to ultra-high performance liquid chromatography analysis.

Analysis conditions: Symmetry 300TM C18 (4.6 mm×250 mm, 5 μm); mobile phase is acetone: 1% acetic acid solution, 0-3min (40:60, v/v), 5-10min (70:30, v/v ), gradient elution, flow rate: 1.5 mL/min; column temperature 40°C, injection volume: 10 μL. The rest are the same as in Example 1, and the analysis results of the components of royal jelly are shown in Table 1.

Example 2

The detection device of the present embodiment is the same as that of Example 1, and the migration amount of the royal jelly component of the special perfuming filter stick is detected by using the above-mentioned detection device, as follows:

Insert a temperature-measuring probe at the beginning of the fragrance-thread-containing filter cigarette of brand B, smoke it on an ordinary smoking machine, and use the temperature-measuring probe to measure the temperature of the smoke passing through the fragrance thread during each puff. And determine the puff number of the whole cigarette.

According to the measured suction temperature and the number of suction ports, set the air temperature in the air heating chamber in the detection device, among which, heating chamber 1' (the first port is 35.4°C), heating chamber 2' (the second port is 36.5°C) , heating chamber 3' (third port 40.1 ℃), heating chamber 4' (fourth port 41.5 ℃), heating chamber 5' (fifth port 43.6 ℃), heating chamber 6' (sixth port 45.2 ℃), heating Chamber 7' (7th port 47.1°C). Set the number of suction ports to 7 in total.

Remove the filter tip of brand B flavored line filter cigarette from the cigarette, install it on the filter holder, start the smoking machine and smoke according to the standard conditions. When taking the first puff, the one that passes through the filter stick is the first. The hot air in the heating chamber; then it is switched by the turntable, and the hot air in the second heating chamber passes through the filter rod when sucking the second port, and so on, and the number of ports is switched in turn. The air flow through the filter simulates the smoke flow when smoking a cigarette, and elutes the fragrance line components.

Use 200ml of methanol-acetonitrile (1:1) solution to carry out flue gas elution and trapping on the simulated flue gas. The collected solution was subjected to ultra-high performance liquid chromatography analysis.

Analysis conditions: Symmetry 300TM C18 (4.6 mm×250 mm, 5 μm); mobile phase is acetone: 1% acetic acid solution, 0-3min (40:60, v/v), 5-10min (70:30, v/v ), gradient elution, flow rate: 1.5mL/min; column temperature 40°C, injection volume: 10μL. The rest are the same as in Example 1, and the analysis results of the components of royal jelly are shown in Table 1.

Example 3

The detection device of the present embodiment is the same as that of Example 1, and the migration amount of the royal jelly component of the special perfuming filter stick is detected by using the above-mentioned detection device, as follows:

Insert a temperature-measuring probe into the initial part of the cigarette with the preformed paper and flavored filter of brand C, smoke on an ordinary smoking machine, and use the temperature-measuring probe to measure the temperature that passes through the initial part of the preformed paper during each puff. The temperature of the smoke, and determine the number of puffs in the whole cigarette.

According to the measured suction temperature and the number of suction ports, set the air temperature in the air heating chamber in the detection device, among which, heating chamber 1' (first port 36.5 ℃), heating chamber 2' (second port 37.9 ℃) , heating chamber 3' (third port 41.2 ℃), heating chamber 4' (fourth port 43.8 ℃), heating chamber 5' (fifth port 45.1 ℃), heating chamber 6' (sixth port 46.7 ℃), heating chamber Chamber 7' (7th port 48.4 ℃), heating chamber 8' (eighth port 50.3 ℃). Set the number of suction ports to 8 in total.

Remove the filter tip of the brand C shaped paper flavored filter cigarette from the cigarette, install it on the filter holder, start the smoking machine and smoke according to the standard conditions. The hot air in the first heating chamber; then switch through the turntable, and the hot air in the second heating chamber passes through the filter rod when sucking the second port, and so on, and the number of ports is switched in turn. The air flow through the filter simulates the smoke flow when the cigarette is smoked, and the components of the flavored molding paper are eluted.

Use 200ml of methanol-acetonitrile (1:1) solution to carry out flue gas elution and trapping on the simulated flue gas. The collected solution was subjected to ultra-high performance liquid chromatography analysis.

Analysis conditions: Symmetry 300TM C18 (4.6 mm×250 mm, 5 μm); mobile phase is acetone: 1% acetic acid solution, 0-3min (40:60, v/v), 5-10min (70:30, v/v ), gradient elution, flow rate: 1.5 mL/min; column temperature 40°C, injection volume: 10 μL. The rest are the same as in Example 1, and the analysis results of the components of royal jelly are shown in Table 1.

Example 4

The detection device of the present embodiment is the same as that of Example 1, and the migration amount of the royal jelly component of the special perfuming filter stick is detected by using the above-mentioned detection device, as follows:

Insert a temperature-measuring probe into the starting part of D-brand filter cigarettes containing granular materials, smoke on an ordinary smoking machine, and use the temperature-measuring probe to measure the amount of smoke that passes through the starting part of the granular material during each puff. temperature, and determine the number of puffs taken throughout the cigarette.

According to the measured suction temperature and the number of suction ports, set the air temperature in the air heating chamber in the detection device, among which, heating chamber 1' (the first port is 35.4°C), heating chamber 2' (the second port is 36.5°C) , heating chamber 3' (third port 40.1 ℃), heating chamber 4' (fourth port 41.5 ℃), heating chamber 5' (fifth port 43.6 ℃), heating chamber 6' (sixth port 45.2 ℃), heating Chamber 7' (7th port 47.1°C). Set the number of suction ports to 7 in total.

Remove the filter tip of the grade D filter cigarette containing particulate material from the cigarette, install it on the filter holder, start the smoking machine and smoke according to the standard conditions. When taking the first puff, the filter stick is the first The hot air in the heating chamber; then it is switched by the turntable, and the hot air in the second heating chamber passes through the filter rod when sucking the second port, and so on, and the number of ports is switched in turn. The air flow through the filter simulates the smoke flow when the cigarette is smoked, and the particulate material components are eluted.

Use 200ml of methanol-acetonitrile (1:1) solution to carry out flue gas elution and trapping on the simulated flue gas. The collected solution was subjected to ultra-high performance liquid chromatography analysis.

Analysis conditions: Symmetry 300TM C18 (4.6 mm×250 mm, 5 μm); mobile phase is acetone: 1% acetic acid solution, 0-3min (40:60, v/v), 5-10min (70:30, v/v ), gradient elution, flow rate: 1.5 mL/min; column temperature 40°C, injection volume: 10 μL. The rest are the same as in Example 1, and the analysis results of the components of royal jelly are shown in Table 1.

Example 5

The detection device of the present embodiment is the same as that of Example 1, and the migration amount of the royal jelly component of the special perfuming filter stick is detected by using the above-mentioned detection device, as follows:

Insert a temperature-measuring probe into the beginning of the filter on a cigarette with an E-brand triacetin flavored filter, smoke on an ordinary smoking machine, and use the temperature-measuring probe to measure the beginning of each puff through the filter The temperature of the smoke, and determine the number of puffs of the whole cigarette.

According to the measured suction temperature and the number of suction ports, set the air temperature in the air heating chamber in the detection device, among which, heating chamber 1' (first port 36.5 ℃), heating chamber 2' (second port 37.9 ℃) , heating chamber 3' (third port 41.2 ℃), heating chamber 4' (fourth port 43.8 ℃), heating chamber 5' (fifth port 45.1 ℃), heating chamber 6' (sixth port 46.7 ℃), heating chamber Chamber 7' (7th port 48.4 ℃), heating chamber 8' (eighth port 50.3 ℃). Set the number of suction ports to 8 in total.

Remove the filter tip of the brand E triacetin flavored filter cigarette from the cigarette, install it on the filter tip holder, start the smoking machine and smoke according to the standard conditions. The hot air in the first heating chamber is the hot air in the first heating chamber; then it is switched through the turntable, and when the second port is sucked, the hot air in the second heating chamber passes through the filter stick, and so on, and the number of ports is switched in turn. The air flow through the filter simulates the smoke flow when smoking a cigarette, and elutes the flavored triacetin component.

The simulated flue gas was eluted and captured with 200ml methanol-acetonitrile (1:1) solution. The collected solution was subjected to ultra-high performance liquid chromatography analysis. Analysis conditions: Symmetry 300TM C18 (4.6 mm×250 mm, 5 μm); mobile phase is acetone: 1% acetic acid solution, 0-3min (40:60, v/v), 5-10min (70:30, v/v ), gradient elution, flow rate: 1.5 mL/min; column temperature 40°C, injection volume: 10 μL. The rest are the same as in Example 1, and the analysis results of the components of royal jelly are shown in Table 1.

Example 6

The detection device of the present embodiment is the same as that of Example 1, and the migration amount of the royal jelly component of the special perfuming filter stick is detected by using the above-mentioned detection device, as follows:

Insert a temperature-measuring probe into the part of the F-brand puffed-pear cigarette where the puffed-puff is installed, smoke it on an ordinary smoking machine, use the temperature-measured probe to measure the temperature of the smoke passing through the puff-puff part during each puff, and determine the temperature of the whole cigarette number of suction ports.

According to the measured suction temperature and the number of suction ports, set the air temperature in the air heating chamber in the detection device, among which, heating chamber 1' (the first port is 35.4°C), heating chamber 2' (the second port is 36.5°C) , heating chamber 3' (third port 40.1 ℃), heating chamber 4' (fourth port 41.5 ℃), heating chamber 5' (fifth port 43.6 ℃), heating chamber 6' (sixth port 45.2 ℃), heating Chamber 7' (7th port 47.1°C). Set the number of suction ports to 7 in total.

Remove the filter tip of the brand F explosive bead cigarette from the cigarette, install it on the filter holder, pinch the explosive bead, start the smoking machine and smoke according to the standard conditions. The hot air in the first heating chamber; then switch through the turntable, when the second port is sucked, the hot air in the second heating chamber passes through the filter rod, and so on, the number of ports is switched in turn. The air flow through the filter simulates the smoke flow when smoking a cigarette, and elutes the added ingredients in the popping beads.

The simulated flue gas was eluted and captured with 200ml methanol-acetonitrile (1:1) solution. The collected solution was subjected to ultra-high performance liquid chromatography analysis.

Analysis conditions: Symmetry 300TM C18 (4.6 mm×250 mm, 5 μm); mobile phase is acetone: 1% acetic acid solution, 0-3min (40:60, v/v), 5-10min (70:30, v/v ), gradient elution, flow rate: 1.5 mL/min; column temperature 40°C, injection volume: 10 μL. The rest are the same as in Example 1, and the analysis results of the components of royal jelly are shown in Table 1.

Example 7

The detection device of the present embodiment is the same as that of Example 1, and the migration amount of the royal jelly component of the special perfuming filter stick is detected by using the above-mentioned detection device, as follows:

Insert a temperature-measuring probe at the beginning of the cigarette with the flavor-filter on G brand cigarettes containing the flavor-filter filter, smoke on an ordinary smoking machine, and use the temperature-measuring probe to measure the temperature of the smoke passing through the flavor-piece during each puff, and determine The number of puffs in a whole cigarette.

According to the measured suction temperature and the number of suction ports, set the air temperature in the air heating chamber in the detection device, among which, heating chamber 1' (first port 36.5 ℃), heating chamber 2' (second port 37.9 ℃) , heating chamber 3' (third port 41.2 ℃), heating chamber 4' (fourth port 43.8 ℃), heating chamber 5' (fifth port 45.1 ℃), heating chamber 6' (sixth port 46.7 ℃), heating chamber Chamber 7' (7th port 48.4 ℃), heating chamber 8' (eighth port 50.3 ℃). Set the number of suction ports to 8 in total.

Remove the filter tip of the brand G cigarette containing flavor filter from the cigarette, install it on the filter holder, start the smoking machine and smoke according to the standard conditions. The hot air in the first heating chamber; then switch through the turntable, and the hot air in the second heating chamber is drawn through the filter rod when sucking the second port, and so on, and the number of ports is switched in turn. The air flow through the filter simulates the smoke flow when smoking a cigarette, and elutes the ingredients of the flavor sheet.

The simulated flue gas was eluted and captured with 200ml methanol-acetonitrile (1:1) solution. The collected solution was subjected to ultra-high performance liquid chromatography analysis. Analysis conditions: Symmetry 300TM C18 (4.6 mm×250 mm, 5 μm); mobile phase is acetone: 1% acetic acid solution, 0-3min (40:60, v/v), 5-10min (70:30, v/v ), gradient elution, flow rate: 1.5 mL/min; column temperature 40°C, injection volume: 10 μL. The rest are the same as in Example 1, and the analysis results of the components of royal jelly are shown in Table 1.

Table 1 Analysis results of royal jelly components

It can be seen from Table 1 that the sample is not affected by complex smoke and can truly reflect the content of royal jelly in the filter.

It can be seen that using the detection device and method of this embodiment can accurately analyze the migration of royal jelly components in special flavoring filters, improve the analysis sensitivity of samples, shorten the detection cycle, and effectively guide product development and design.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the scope of the present invention. within the scope of protection.

Claims (4)

1. the detection method of royal jelly ingredient the amount of migration in a kind of filter stick, it is characterised in that：Include the following steps：

Step（1）, puff number determine

It is inserted into thermoprobe at the remote lip end charging starting position containing Flavoring filter of cigarette to be measured, is taken out on conventional smoking machine It inhales, originates the temperature of position flue gas when measuring every mouthful of suction by thermoprobe by Flavoring filter, and determine whole branch cigarette Puff number；

Step（2）, according to puff number set needed for heating chamber temperature

The air themperature in several heating chambers of detection device is set by the practical suction temperatures of cigarette, and sets puff number；

Step（3）, suction

Smoking machine, Fume collector and filter tip clamper are sequentially connected, the filter tip of extraordinary filter tip cigarette is taken from cigarette Under, it is installed on filter tip clamper, starts smoking machine and aspirated by standard conditions, heating of the air from different temperatures of suction Chamber switches successively according to puff number, and every mouthful of gas flow temperature for being aspirated through filter tip is and when the practical suction of cigarette smoking machine Gas flow temperature it is consistent；Flue gas elution trapping is carried out to simulated flue gas using methanol-acetonitrile solution；

Step（4）, constituent analysis；

After trapping, ultra performance liquid chromatography analysis, analysis condition are carried out to trapping solution：

Symmetry 300TM C18,4.6 mm × 250 mm, 5 μm；

Mobile phase is acetone：1% acetum；0-3min is 40：60, v/v；5-10min is 70：30, v/v, gradient elution；Stream Speed: 1.5 mL/min；40 DEG C of column temperature, sample size：10μL.

2. detection method according to claim 1, it is characterised in that：It is detected using detection device, the detection dress It sets including several heating chambers, heating chamber one end connects air inlet, and the other end connects escape pipe, and escape pipe is arranged around turntable, turns Include the first air inlet pipe on disk, the first air inlet pipe is rotated with turntable, and first air inlet pipe one end at a time goes out with one of them Tracheae is connected to, and the first air inlet pipe other end is connected to second air inlet pipe one end, and the second air inlet pipe is fixed, and the other end is clamped with filter tip Device connects, and filter tip clamper connects Fume collector, and Fume collector connects smoking machine.

3. detection method according to claim 1, it is characterised in that：Heating chamber is 10, and temperature is equipped in heating chamber Regulating device, heating temperature range are 30-200 DEG C.

4. detection method according to claim 1, it is characterised in that：Step（4）In, using 200ml methanol-acetonitrile solution Flue gas elution trapping is carried out to simulated flue gas, wherein the volume ratio of methanol-acetonitrile is 1：1.