CN108586116A - A kind of biological organic fertilizer and preparation method thereof of prevention pepper ralstonia solanacearum - Google Patents
A kind of biological organic fertilizer and preparation method thereof of prevention pepper ralstonia solanacearum Download PDFInfo
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- CN108586116A CN108586116A CN201810907519.6A CN201810907519A CN108586116A CN 108586116 A CN108586116 A CN 108586116A CN 201810907519 A CN201810907519 A CN 201810907519A CN 108586116 A CN108586116 A CN 108586116A
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- ralstonia solanacearum
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Fertilizers (AREA)
Abstract
The present invention provides a kind of biological organic fertilizer and preparation method thereof of prevention pepper ralstonia solanacearum, belongs to technical field of fertilizers, includes the raw material of following parts by weight：50~150g of luffa, 2~20g of yeast, 10~30g of ferment, 10~30g of activated biomass, 20~50g of N P and K ternary compound fertilizer, 5~15g of non-pathogenic Ralstonia solanacearum.The present invention passes through the microbial synergies such as carrier luffa and yeast, ferment, symbiosis is formed with capsicum, yeast and ferment decompose the field planting of the nutriment and microorganism that generate, improve capsicum Nutrient Absorption efficiency, then the ecological niche of Ralstonia solanacearum is occupied using non-pathogenic Ralstonia solanacearum, the proliferation of pathogen, biological organic fertilizer of the invention is effectively inhibited to can be used for improving Quality of Capsicum and increase its yield.
Description
Technical field
The present invention relates to technical field of fertilizers, more particularly to the biological organic fertilizer of a kind of prevention pepper ralstonia solanacearum and its preparation
Method.
Background technology
Capsicum is the important industrial crops in China, is that the main vegetables of daily life congratulate flavouring.With Ralstonia solanacearum
Based on soil-borne disease be restrict capsicum production an important factor for, in order to improve yield of hot pepper and prevention capsicum pest and disease damage, plantation
Family puts into a large amount of chemical fertilizer and pesticide in planting process, with a large amount of applications of chemical fertilizer, pesticide, is ensureing and increasing crop life
While long, a series of adverse effects are brought to soil environment and crop, soil is made to lack organic matter, hardened, acidification, adherency
Power decrease, nutrient loss, crop cannot be metabolized chemical fertilizer element etc. completely.In order to reduce chemical fertilizer and applications of pesticide amount, have developed
Specific microorganism, vegetable-derived components are added to and form novel, efficient, safe microorganism-in organic fertilizer by biological organic fertilizer
Organic manure is rich in a large amount of benefit materials, including：A variety of organic acids, peptides and enriching including nitrogen, phosphorus, potassium
Nutrient, nutrition can not only be provided for crops, and fertilizer efficiency is long, can increase and update the soil organism, promote micro- life
Object is bred, and the physicochemical property and bioactivity of soil are improved, but existing capsicum is not high with microbial manure fertilizer efficiency, and is retained
Poor performance.
China Patent No. CN201710289455.3 discloses a kind of capsicum Special compound microbial fertilizer and its preparation side
Method, including organic fertilizer component, inorganic fertilizer component, complex microorganism component and chitosan oligosaccharide；The disease-resistant of plant is induced using chitosan oligosaccharide
Property, being conducive to capsicum resists powdery mildew, epidemic disease etc., and still, chitosan oligosaccharide has broad-spectrum antibacterial activity, not only inhibits harmful to capsicum
Microorganism, certain killing effect is also will appear to beneficial microbe, to make structure of soil microbial community lack of proper care；Its
Secondary, bacteriostasis makes it weaken the fermentation of organic fertilizer component to a certain degree, reduces organic substance and nutrient, and cannot press down completely
Crops are caused secondary pollution by the pathogen in system, elimination excrement；Furthermore the loss of microorganism in the actual production process
Bigger, not easy to maintain, effective bacterial content is too low, keeps its getting fat resistant effect limited；Separately there is China Patent No.
CN201310097453.6 disclose it is a kind of can laying hen manure organic fertilizer capable of preventing and removing pepper phytophthora blight and pepper anthracnose production method,
Bacterial strain-microorganism Aspergillus aculeatus is combined using biological control and skin falls mould and prevents capsicum epidemic disease, is reduced to a certain degree to the micro- life of soil
The influence of object structure of community, but bacterial strain uses therefor has a single function, and lacks decomposition of the microorganism to organic matter, fertilizer efficiency is caused to drop
It is low, reduce absorption efficiency of the capsicum to nutriment.
Therefore, exploitation can either increase soil fertility, and improve soil, preserve the ecological environment, and improve crop yield and quality,
The biological organic fertilizer of the soil-borne disease of energy antagonism crop again, for promoting agricultural benign cycle and sustainable development with important meaning
Justice.
Invention content
The purpose of the present invention is to provide a kind of biological organic fertilizers and preparation method thereof of prevention pepper ralstonia solanacearum, pass through load
The microbial synergies such as body luffa and yeast, ferment form symbiosis with capsicum, and yeast and ferment decompose the nutrients generated
The field planting of matter and microorganism improves capsicum Nutrient Absorption efficiency, is then occupied using non-pathogenic Ralstonia solanacearum green withered
The ecological niche of bacterium effectively inhibits the proliferation of pathogen, biological organic fertilizer of the invention to can be used for improving Quality of Capsicum and increase
Its yield.
The present invention above-mentioned technical purpose technical scheme is that：
A kind of biological organic fertilizer of prevention pepper ralstonia solanacearum, includes the raw material of following parts by weight：It is 50~150g of luffa, big
2~20g of song, 10~30g of ferment, 10~30g of activated biomass, 20~50g of N P and K ternary compound fertilizer, non-pathogenic Solanaceae labor
You are 5~15g of Salmonella.
Further, the luffa is magnetic luffa.
Further, the activated biomass is at least two in humus, yolk, six ring stones.
Further, the N P and K ternary compound fertilizer is mixed by plant ash, calcium superphosphate, urea.
Further, plant ash, calcium superphosphate, urea adding proportion (by mass) be 3~5：1：5.
A kind of preparation method of the biological organic fertilizer of prevention pepper ralstonia solanacearum, includes the following steps：
S1, by ripe loofah peeling, remove seed, cut both ends narrower portion, it is 0.5~2cm left that remainder, which is cut into thickness,
Right column；
S2, daqu fermentation liquid, shaken cultivation is added in luffa；
S3, ferment zymotic fluid, non-pathogenic Ralstonia solanacearum zymotic fluid are uniformly sprayed onto in step S2 load have it is big
On the luffa of bent microorganism, activated biomass is added and is uniformly mixed, adjusts water content 50~70%, compost fermentation；
S4, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S5, by moisture evaporation to water content be 30~40% when, be granulated, pack, quality inspection, certified products sealing storage.
Further, the luffa is magnetic luffa, and preparation method is：Deionized water is added in the reactor
100g、Fe3O40.2g、MgSO4 0.15g、MnSO4 0.15g、CoCl2 0.2g、ZnSO40.2g, ultrasonic disperse at suspension,
Luffa is added, mixed solution is ultrasonically treated, then filters, is dry, obtaining magnetic luffa.
Further, the condition of the step S2 shaken cultivations is：25~37 DEG C, 40~100rpm, shaken cultivation 48~
72h。
Further, the condition of the step S3 compost fermentations is：First aerobic fermentation 2d, 20~30d of subsequent decomposed fermentation,
Period is primary per 2d turnings.
A kind of application of the biological organic fertilizer of prevention pepper ralstonia solanacearum, the biological organic fertilizer is for inhibiting capsicum blueness withered
Disease.
The beneficial effects of the invention are as follows：
1. using luffa as carrier, luffa has larger specific surface area, flourishing pore structure and degradable right
It is environmental-friendly, it is added in organic fertilizer, water retention in soil and the confinement capabilities to nutrient, increase organic carbon can be improved
Accumulation and influence microbial population development and stable state and enhance improve soil microbe quantity and activity etc., Jin Ergai
Kind soil aggregate structure, oxygen content, aeration status, fertility etc., promote plant growth, improve yield.
2. adsorbing the microorganism in ferment and yeast by luffa is fixed to luffa surface, one improves biology
Amount, microorganism is stable and not easily runs off, and resistance to murder by poisoning ability is strong, and two carry out surface aggregation microorganism degrades under aerobic/anoxia condition
Organic matter in soil increases the content of available N and P, potassium, sulphur etc. in soil, increase ASI systematic approach, while inside
Microbial degradation loofah fiber is bred for the microbe survival on surface, and the activity of being kept fixed microorganism is unattenuated, until
Luffa degradation is finished, in the microorganism Spontaneous release to soil of absorption, increases diversity of soil microorganism.
3. first co-culturing yeast and luffa, ferment compost is added, to make the microorganism in yeast and luffa
It comes into full contact with, is adsorbed in the porous structure of luffa, the high active enzyme of secretion makes the cellulose dissolution of luffa and secretes one
A little biotic factors etc., provide nutrient for follow-up ferment Microorganism colonization, can also make the cell membrane swelling of plant duff
Softening destroys, and makes to contain the protein in cell and carbohydrate release, is decomposed for ferment, then improve nutrition in soil
The utilization rate of element, and promote raw material quick composting, shorten the compost time；Secondly, by the degradation of enzyme to luffa into
Row is modified, and makes its rough surface, very Big mutation rate occurs for hole, small to one nanometer, greatly to tens nanometers or even some tens of pm, shape
The microenvironment that life can be inhabited at microorganism is conducive to the attachment of follow-up ferment microorganism and non-pathogenic Ralstonia solanacearum；
The unformed partial hydrolysis of another aspect fiber makes the hole of crystal region become larger, and helps to improve water-retaining property and water absorption.
4. can effectively inhibit the proliferation of pathogen using non-pathogenic Ralstonia solanacearum as biocontrol microorganisms, deposited with Ralstonia solanacearum
In higher niche overlap, therefore Ralstonia solanacearum available resources can be made to reduce by preferentially seizing the resource in rhizosphere soil,
Achieve the purpose that inhibit Ralstonia solanacearum, and there is no the nutrient competition with other microorganisms, does not influence the ecologic structure of indigenous flora.
5. forming symbiosis by the way that the microorganisms such as yeast, ferment and capsicum are added, yeast and ferment decompose the battalion generated
The field planting for supporting substance and microorganism, have stimulated the root growth of capsicum and increases root absorption area, improve the nutrient of capsicum
Then absorption efficiency occupies the ecological niche of Ralstonia solanacearum using non-pathogenic Ralstonia solanacearum, effectively inhibit the proliferation of pathogen,
The growth and development for promoting capsicum can be used for improving Quality of Capsicum and increases its production in biological organic fertilizer agriculturally of the invention
Amount, there is huge economic benefit.
Specific implementation mode
Technical scheme of the present invention is clearly and completely described below in conjunction with specific embodiment.Obviously, described
Embodiment be only the present invention a part of the embodiment, instead of all the embodiments.Based on the embodiment of the present invention, ability
The every other embodiment that domain those of ordinary skill is obtained without creative efforts, belongs to guarantor of the present invention
The range of shield.
A kind of biological organic fertilizer of prevention pepper ralstonia solanacearum, includes the raw material of following parts by weight：It is 50~150g of luffa, big
2~20g of song, 10~30g of ferment, 10~30g of activated biomass, 20~50g of N P and K ternary compound fertilizer, non-pathogenic Solanaceae labor
You are 5~15g of Salmonella.
Luffa is the natural vascular bundle that ripe sponge gourd fruit removes the cellulosic reticular structure obtained after crust and seed
Tissue, is made of 60% cellulose, 30% hemicellulose and 10% lignin, has on morphosis continuous hollow
Microcellular structure, formed vascular bundle simultaneously generate multichannel and multistage pore system, this unique space structure makes its large specific surface area,
Specific surface area average value is up to 123m2/ g, and adsorption capacity is strong, water absorption is big, is 3 times or so of material dead weight, mean water absorption
Rate 2106g/m2, be averaged resolution factor 98.27%, environmentally safe.The present invention using luffa as microbe carrier have with
Lower benefit：
First, luffa rough surface has porous structure, provides habitat and space for the existence of microorganism, be
The growth and breeding of peculiar microorganism provides hotbed, increases Soil Biodiversity, while regulating and controlling the physics and chemistry of Soil Micro-environment
Property stores moisture and nutrient, influences and regulate and control the growth and development and metabolism of edaphon, and then improve soil fertility；Together
The fiber of Shi Zuowei microbe carriers, luffa is relatively high to the compatibility of microorganism, is conducive to microorganism attachment, biological load
Amount is high, and cellulose is degraded during soil reaction, and meeting slow release carbon source supplies microorganism growth and development and metabolism,
Conducive to microbial activities, soil microbe quantity and activity can be improved.
Second, after the vesicular texture of luffa, suitable porosity make it apply farmland, be conducive to increase soil total
Porosity, capillary porosity and aeration porosity can quickly absorb moisture to improve the wicking of soil, and be maintained at soil
In earth hole, the effective moisture content and moisture holding capacity of soil are improved, and luffa is rich in cellulose, hemicellulose and lignin etc.
Hydroaropic substance is also beneficial to preserve moisture.
Third, luffa delay biological organic fertilizer release in the soil and leaching loss as carrier sticking part nutrient；Silk
Melon network generates absorption to mineral cation by its surface acidity functional group and is acted on withholding, reduces the molten of water-soluble nutrient ions
Solution migration avoids the leaching loss of nutrient, and continues in the soil and slowly discharge, and is equivalent to the sustained release of nutrient
Carrier improves utilization rate of fertilizer to achieve the effect that keep fertility；Secondly, luffa is similar to sponge state, is sent out in organic fertilizer
During ferment, microcosmic effect can be carried out with other material compositions, the loose and porous structure having can improve organic fertilizer
Structure makes organic fertilizer after shaping, and sponge state institutional framework also is presented can be effective after entering applying fertilizer in soil
The hardened state for improving soil, forms loose porous soil texture, improves oxygen content, oxidation-reduction quality and the ventilation shape of soil
Condition etc., to increase the yield of soil fertility and crops.
4th, soil granular is soil organic colloid such as humus and inorganic colloid such as calcium carbonate, calcium sulfate etc. by more
The electrostatic interaction of valence cation, which bonds, to be formed, after luffa adsorbs mineral cation, be agron, activated biomass,
Calcium superphosphate etc. provides abundant bridging substance-bivalent cation, and the electrostatic attraction for making it through positive and negative charge quickly agglomerates,
Soil organic-inorganic glue compound is formed, soil granular is promoted to be formed, the formation of soil granular can keep soil from packing together, and preserve
Moisture, the consumption and accumulation for coordinating nutrient reduce nutrition leak, provide tilth to a soil.
In short, luffa has larger specific surface area, flourishing pore structure and degradable environmentally friendly, addition
Into organic fertilizer, water retention in soil can be improved and to the confinement capabilities of nutrient, the accumulation of increase organic carbon and shadow
The development of sound microbial population and stable state and the activity etc. for enhancing edaphon, and then improve soil texture, oxygen content, lead to
Vaporous condition, fertility etc. promote plant growth.But to promote the increase of crop yield, it is to be unable to reach that luffa, which is administered alone,
, need the synergistic effect of mineral nutrient element and microorganism such as ferment.
Ferment, also known as ferment fertilizer are a kind of microbial manures, by nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, saccharomycete, unwrapping wire
What bacterium, fungi formed can generate the profitable strains of a variety of catalytic decomposition enzymes, these biological floras energy in cultivating reproductive process
Ten several inhomogeneous enzymes such as amylase, protease, lipase, lactalase are generated, catalytic decomposition weeds, plant duff etc. have
Machine substance, and the chemical substances such as chemical fertilizer, pesticide can be decomposed, the minerals such as shale, zeolite, bentonite can also be decomposed, are allowed to
The active ingredient utilized for plant is converted into short time, release stores a large amount of fixed phosphorus potassium and micro member in the soil
Element increases soil nutrient availability, while microorganism breeds with surprising rapidity in the soil, and the secretion of generation can promote soil
The formation of earth crumb structure, eliminates hardened, and then increases soil permeability, water-retaining property, builds up fertility with sustainability etc., improving
Crop growth environment, the effect for reducing fertilizer and pesticide amount.
Yeast, also known as block are bent or brick is bent, for a kind of raw material made wine, with barley, wheat, pea etc. for raw material, pass through
It crushes, adds water kneading, be pressed into bent unstrained spirits, the various microorganisms of nature is allowed to grow and be made above.Yeast pectase, hemicellulose
Enzyme, cellulase, protease, the enzyme activity of carbohydrase are higher, and pectase, cellulase etc. can make the cellulose of luffa molten
Solution, nutrition is provided for follow-up ferment Microorganism colonization, and the cell membrane swelling of plant duff can also be made to soften and destroyed, make to contain
Protein in cell and carbohydrate release, decompose for ferment, then improve the utilization rate of Nutrient Elements in Soil,
It is secondary, luffa is modified.
Non-pathogenic Ralstonia solanacearum can effectively inhibit the proliferation of pathogen as biocontrol microorganisms, exist with Ralstonia solanacearum
Higher niche overlap, therefore by preferentially seizing the resource in rhizosphere soil, so that Ralstonia solanacearum available resources is reduced, reach
Inhibit the purpose of Ralstonia solanacearum.Under the conditions of optimum temperature and nutrient concentration, suppression of the non-pathogenic Ralstonia solanacearum to Ralstonia solanacearum
Ability processed is strong.
The present invention forms symbiosis by the way that the microorganisms such as yeast, ferment and capsicum are added, and yeast and ferment, which decompose, to be generated
Nutriment and microorganism field planting, have stimulated the root growth of capsicum and increase root absorption area, improve capsicum
Nutrient Absorption efficiency, resist capsicum root system it is unsound phenomena such as, then occupied using non-pathogenic Ralstonia solanacearum green withered
The ecological niche of bacterium effectively inhibits the proliferation of pathogen, chili growth development is promoted, in biological organic fertilizer agriculturally of the invention
It can be used for improving the quality of capsicum and increase its yield, there is huge economic benefit.
The luffa is magnetic luffa.First luffa is immersed in solion, keeps its adsorption certain
Metal cation helps to activate cellulase, improves enzymatic activity, shortens the compost time.
The activated biomass is at least two in humus, yolk, six ring stones.Humus is organic matter through microorganism
The colloidal substance that decomposition and inversion is formed, generally black or burgundy, are the chief component of the soil organism, humus master
It to be made of nutrients such as carbon, hydrogen, oxygen, nitrogen, sulphur, phosphorus, humus has the cohesiveness of appropriateness, is to form crumb structure good
Good cementing agent, secondly, the antimicrobial effect of eating of humus energy keep the soil organism horizontal, but also help guarantee nitrogen
With other required nutrition, these all exist in humus complexs combination of antidecomposition.The part turned to be yellow inside yolk, that is, egg,
Rich in trace elements such as liposoluble vitamin, monounsaturated fatty acids, phosphatide, phosphorus, iron, yolk is in addition to being rich in nutrient, energy
It dramatically increases outside the content of organic matter in soil, the phosphatide being rich in has certain protective effect to microorganism, can be improved micro-
The activity of biology.Six ring stones are the condensates with four big energy：Energy one, the far infrared that launch wavelength is 8-15 μm, make
For water, can activated water molecule, so that water clusters is become smaller, be more suitable for soil absorption, act on microorganism, ferment is promoted to generate,
Activate microbial cell；Redox reaction, and coupling collar easily occur for energy two, permanent generating negative ions, variable valency metal Fe
Hydrone in border forms a large amount of anion, has the function of inhibiting or killing soil-borne disease bacterium；Energy three, six ring stones contain
The micro- nutrients such as " germanium " and calcium, magnesium, sodium, potassium, iron, selenium, zinc, improve soil ion type；Energy four generates biology
Electricity.
The N P and K ternary compound fertilizer is mixed by plant ash, calcium superphosphate, urea.Improve soil using plant ash
And supplement potash fertilizer, calcium superphosphate supplement phosphate fertilizer, urea supplements nitrogenous fertilizer.
The plant ash, calcium superphosphate, urea adding proportion (by mass) be 3~5：1：5.
A kind of preparation method of the biological organic fertilizer of prevention pepper ralstonia solanacearum, includes the following steps：
S1, by ripe loofah peeling, remove seed, cut both ends narrower portion, it is 0.5~2cm left that remainder, which is cut into thickness,
Right column；
S2, daqu fermentation liquid, shaken cultivation is added in luffa；
S3, ferment zymotic fluid, non-pathogenic Ralstonia solanacearum zymotic fluid are uniformly sprayed onto in step S2 load have it is big
On the luffa of bent microorganism, activated biomass is added and is uniformly mixed, adjusts water content 50~70%, compost fermentation；
S4, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S5, by moisture evaporation to water content be 30~40% when, be granulated, pack, quality inspection, certified products sealing storage.
The present invention compared with the prior art in, will each raw material mix after carry out compost fermentation together, have the advantages：First
Yeast and luffa are co-cultured, ferment compost is added, to make the microorganism in yeast and luffa come into full contact with, absorption
In the porous structure of luffa, and using luffa as nutriment, the degradation such as eccrine fiber element enzyme, pectase, carbohydrase is fine
Tie up the carbohydrate that element is small molecule and secrete some biotic factors etc., nutrient is provided for follow-up ferment Microorganism colonization,
It is secondary, luffa is modified by the degradation of enzyme, after enzymolysis, a large amount of pectin, cellulose dissolution, lignin are also gone
It removes, surface is relatively rough, and very Big mutation rate occurs for hole, small to one nanometer, greatly to tens nanometers or even some tens of pm, becomes micro-
Biology can inhabit the microenvironment of life, be conducive to the attachment of follow-up ferment microorganism and non-pathogenic Ralstonia solanacearum；It is another
The unformed partial hydrolysis of aspect fiber makes the hole of crystal region become larger, and helps to improve water-retaining property and water absorption.
The present invention adsorbs the microorganism in ferment and yeast by luffa and is fixed to luffa surface, and one improves
Biomass, microorganism is stable and not easily runs off, and resistance to murder by poisoning ability is strong, and two carry out surface aggregation microorganism under aerobic/anoxia condition
Organic matter in degradation soil, increases the content of available N and P, potassium, sulphur etc. in soil, increases ASI systematic approach, while interior
The microbial degradation loofah fiber in portion is bred for the microbe survival on surface, and the activity of being kept fixed microorganism is unattenuated,
Until luffa degradation is finished, in the microorganism Spontaneous release to soil of absorption, increase diversity of soil microorganism.
The luffa is magnetic luffa, and preparation method is：Deionized water 100g, Fe is added in the reactor3O4
0.2g、MgSO4 0.15g、MnSO4 0.15g、CoCl2 0.2g、ZnSO4Sponge gourd is added at suspension in 0.2g, ultrasonic disperse
Network is ultrasonically treated mixed solution, then filters, is dry, obtaining magnetic luffa.
The condition of the step S2 shaken cultivations is：25~37 DEG C, 40~100rpm, 48~72h of shaken cultivation.
The condition of the step S3 compost fermentations is：First aerobic fermentation 2d, 20~30d of subsequent decomposed fermentation, during which per 2d
Turning is primary.
A kind of application of the biological organic fertilizer of prevention pepper ralstonia solanacearum, the biological organic fertilizer is for inhibiting capsicum blueness withered
Disease.
One, embodiment
Embodiment 1
A kind of biological organic fertilizer of prevention pepper ralstonia solanacearum, includes the raw material of following parts by weight：Luffa 100g, yeast
10g, ferment 20g, activated biomass 20g (six ring stone 10g, yolk 10g), N P and K ternary compound fertilizer 35g, non-pathogenic Solanaceae
Raul Salmonella 10g, the N P and K ternary compound fertilizer are mixed by plant ash, calcium superphosphate, urea, plant ash, peroxophosphoric acid
Calcium, urea adding proportion (by mass) be 4：1：5；Preparation method includes the following steps：
S1, by ripe loofah peeling, remove seed, cut both ends narrower portion, it is 0.5~2cm left that remainder, which is cut into thickness,
Right column；
S2, daqu fermentation liquid, 30 DEG C, 70rpm, shaken cultivation 60h is added in luffa；
S3, ferment zymotic fluid, non-pathogenic Ralstonia solanacearum zymotic fluid are uniformly sprayed onto in step S2 load have it is big
On the luffa of bent microorganism, activated biomass is added and is uniformly mixed, adjusting water content 50~70%, first aerobic fermentation 2d, with
Decomposed fermentation 25d afterwards, it is during which primary per 2d turnings；
S4, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S5, by moisture evaporation to water content be 30~40% when, be granulated, pack, quality inspection, certified products sealing storage.
Embodiment 2
A kind of biological organic fertilizer of prevention pepper ralstonia solanacearum, includes the raw material of following parts by weight：It is magnetic luffa 50g, big
Bent 2g, ferment 10g, activated biomass 10g (humus 5g, yolk 5g), N P and K ternary compound fertilizer 50g, non-pathogenic Solanaceae
Raul Salmonella 5g, the N P and K ternary compound fertilizer are mixed by plant ash, calcium superphosphate, urea, plant ash, peroxophosphoric acid
Calcium, urea adding proportion (by mass) be 3：1：5；Preparation method includes the following steps：
S1, by ripe loofah peeling, remove seed, cut both ends narrower portion, it is 0.5~2cm left that remainder, which is cut into thickness,
Right column；
S2, magnetic luffa is prepared：Deionized water 100g, Fe is added in the reactor3O4 0.2g、MgSO4 0.15g、
MnSO40.15g、C℃l2 0.2g、ZnSO40.2g, ultrasonic disperse are added luffa, surpass to mixed solution at suspension
Then sonication filters, is dry, obtaining magnetic luffa；
S3, daqu fermentation liquid, 25 DEG C, 40rpm, shaken cultivation 72h is added in magnetic luffa；
S4, ferment zymotic fluid, non-pathogenic Ralstonia solanacearum zymotic fluid are uniformly sprayed onto in step S2 load have it is big
On the luffa of bent microorganism, activated biomass is added and is uniformly mixed, adjusting water content 50~70%, first aerobic fermentation 2d, with
Decomposed fermentation 30d afterwards, it is during which primary per 2d turnings；
S5, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S6, by moisture evaporation to water content be 30~40% when, be granulated, pack, quality inspection, certified products sealing storage.
Embodiment 3
A kind of biological organic fertilizer of prevention pepper ralstonia solanacearum, includes the raw material of following parts by weight：It is magnetic luffa 80g, big
Bent 6g, ferment 15g, activated biomass 15g ((humus 8g, yolk 7g)), N P and K ternary compound fertilizer 42g, non-pathogenic eggplant
Section Raul Salmonella 8g, the N P and K ternary compound fertilizer are mixed by plant ash, calcium superphosphate, urea, plant ash, peroxophosphoric acid
Calcium, urea adding proportion (by mass) be 4：1：5；Preparation method includes the following steps：
S1, by ripe loofah peeling, remove seed, cut both ends narrower portion, it is 0.5~2cm left that remainder, which is cut into thickness,
Right column；
S2, magnetic luffa is prepared：Deionized water 100g, Fe is added in the reactor3O4 0.2g、MgSO4 0.15g、
MnSO40.15g、C℃l2 0.2g、ZnSO40.2g, ultrasonic disperse are added luffa, surpass to mixed solution at suspension
Then sonication filters, is dry, obtaining magnetic luffa；
S3, daqu fermentation liquid, 28 DEG C, 60rpm, shaken cultivation 66h is added in magnetic luffa；
S4, ferment zymotic fluid, non-pathogenic Ralstonia solanacearum zymotic fluid are uniformly sprayed onto in step S2 load have it is big
On the luffa of bent microorganism, activated biomass is added and is uniformly mixed, adjusting water content 50~70%, first aerobic fermentation 2d, with
Decomposed fermentation 28d afterwards, it is during which primary per 2d turnings；
S5, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S6, by moisture evaporation to water content be 30~40% when, be granulated, pack, quality inspection, certified products sealing storage.
Embodiment 4
A kind of biological organic fertilizer of prevention pepper ralstonia solanacearum, includes the raw material of following parts by weight：It is magnetic luffa 100g, big
Bent 10g, ferment 20g, activated biomass 20g (humus 10g, yolk 10g), N P and K ternary compound fertilizer 35g, non-pathogenic eggplant
Section Raul Salmonella 10g, the N P and K ternary compound fertilizer are mixed by plant ash, calcium superphosphate, urea, and plant ash crosses phosphorus
Sour calcium, urea adding proportion (by mass) be 4：1：5；Preparation method includes the following steps：
S1, by ripe loofah peeling, remove seed, cut both ends narrower portion, it is 0.5~2cm left that remainder, which is cut into thickness,
Right column；
S2, magnetic luffa is prepared：Deionized water 100g, Fe is added in the reactor3O4 0.2g、MgSO4 0.15g、
MnSO40.15g、C℃l2 0.2g、ZnSO40.2g, ultrasonic disperse are added luffa, surpass to mixed solution at suspension
Then sonication filters, is dry, obtaining magnetic luffa；
S3, daqu fermentation liquid, 30 DEG C, 70rpm, shaken cultivation 60h is added in magnetic luffa；
S4, ferment zymotic fluid, non-pathogenic Ralstonia solanacearum zymotic fluid are uniformly sprayed onto in step S2 load have it is big
On the luffa of bent microorganism, activated biomass is added and is uniformly mixed, adjusting water content 50~70%, first aerobic fermentation 2d, with
Decomposed fermentation 25d afterwards, it is during which primary per 2d turnings；
S5, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S6, by moisture evaporation to water content be 30~40% when, be granulated, pack, quality inspection, certified products sealing storage.
Embodiment 5
A kind of biological organic fertilizer of prevention pepper ralstonia solanacearum, includes the raw material of following parts by weight：It is magnetic luffa 120g, big
Bent 15g, ferment 25g, activated biomass 25g (humus 15g, six ring stone 10g), N P and K ternary compound fertilizer 27g, non-pathogenic
Ralstonia solanacearum 13g, the N P and K ternary compound fertilizer are mixed by plant ash, calcium superphosphate, urea, plant ash, mistake
Calcium phosphate, urea adding proportion (by mass) be 4：1：5；Preparation method includes the following steps：
S1, by ripe loofah peeling, remove seed, cut both ends narrower portion, it is 0.5~2cm left that remainder, which is cut into thickness,
Right column；
S2, magnetic luffa is prepared：Deionized water 100g, Fe is added in the reactor3O4 0.2g、MgSO4 0.15g、
MnSO40.15g、C℃l2 0.2g、ZnSO40.2g, ultrasonic disperse are added luffa, surpass to mixed solution at suspension
Then sonication filters, is dry, obtaining magnetic luffa；
S3, daqu fermentation liquid, 32 DEG C, 85rpm, shaken cultivation 54h is added in magnetic luffa；
S4, ferment zymotic fluid, non-pathogenic Ralstonia solanacearum zymotic fluid are uniformly sprayed onto in step S2 load have it is big
On the luffa of bent microorganism, activated biomass is added and is uniformly mixed, adjusting water content 50~70%, first aerobic fermentation 2d, with
Decomposed fermentation 23d afterwards, it is during which primary per 2d turnings；
S5, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S6, by moisture evaporation to water content be 30~40% when, be granulated, pack, quality inspection, certified products sealing storage.
Embodiment 6
A kind of biological organic fertilizer of prevention pepper ralstonia solanacearum, includes the raw material of following parts by weight：It is magnetic luffa 150g, big
Bent 20g, ferment 30g, activated biomass 30g (yolk 15g, six ring stone 15g), N P and K ternary compound fertilizer 20g, non-pathogenic eggplant
Section Raul Salmonella 15g.The N P and K ternary compound fertilizer is mixed by plant ash, calcium superphosphate, urea, and plant ash crosses phosphorus
Sour calcium, urea adding proportion (by mass) be 5：1：5；Preparation method includes the following steps：
S1, by ripe loofah peeling, remove seed, cut both ends narrower portion, it is 0.5~2cm left that remainder, which is cut into thickness,
Right column；
S2, magnetic luffa is prepared：Deionized water 100g, Fe is added in the reactor3O4 0.2g、MgSO4 0.15g、
MnSO40.15g、C℃l2 0.2g、ZnSO40.2g, ultrasonic disperse are added luffa, surpass to mixed solution at suspension
Then sonication filters, is dry, obtaining magnetic luffa；
S3, daqu fermentation liquid, 35 DEG C, 100rpm, shaken cultivation 48h is added in magnetic luffa；
S4, ferment zymotic fluid, non-pathogenic Ralstonia solanacearum zymotic fluid are uniformly sprayed onto in step S2 load have it is big
On the luffa of bent microorganism, activated biomass is added and is uniformly mixed, adjusting water content 50~70%, first aerobic fermentation 2d, with
Decomposed fermentation 20d afterwards, it is during which primary per 2d turnings；
S5, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S6, by moisture evaporation to water content be 30~40% when, be granulated, pack, quality inspection, certified products sealing storage.
Two, experimental example
It is common vegetable field soil, basic physical and chemical pH6.3, organic matter 20.2g/kg, full nitrogen for examination soil
1.62g/Kg, full phosphorus 0.61g/Kg, full potassium 24.5g/Kg fill soil 15kg per basin using pot experiment, plant 4 plants of capsicum, Miao Zuo
Spacing under upper right is 10cm, and experiment is divided into 8 processing, 10 repetitions of each processing setting；By pepper planting conventional method pipe
Reason waits for measuring the influence to yield after its influence and final result to chili growth situation before capsicum blooms, and according to upper
It states experiment to repeat to do pot experiment, is inoculated with capsicum Ralstonia solanacearum, observing pepper ralstonia solanacearum, a situation arises.
Comparative example 1：It does not apply fertilizer, is control group；
Comparative example 2：N, P, K fertilizer are applied, amount of application is urea 8g/ basins, and Diammonium phosphate (DAP) 15g/ basins, potassium phosphate is 5g/ basins；
Embodiment 1-6：Dose be 20g/ basins, living bacteria count >=2.5 hundred million/gram；
Influence of 1 different disposal of table to chili growth and yield
A situation arises to pepper ralstonia solanacearum for 2 different disposal of table
Upper table 1 is the manure trial of embodiment 1-6, comparative example 1-2, it can be deduced that biological organic fertilizer processing of the invention
The total output of capsicum has different degrees of increase, the yield of hot pepper of Processing Example 4 to improve at most, reach with contrast ratio
206g, the control group that less applies fertilizer improve 64.8%；The each measurement index analysis of capsicum plant height, stem thickness, root fresh weight is apparent to be compared
The production effect of ratio 1-2 is good, shows that applying biological organic fertilizer processing has chili growth different degrees of facilitation；It is former
Because being to apply bio-organic fertilizer to improve the character of soil fertility and pepper plant, keep soil nutrient sufficient, crop inhales
A large amount of nutriment is received, to make to improve upgrowth situation, yield increases.
Upper table 2 is the bacterial wilt controlling experiment of embodiment 1-6, comparative example 1-2, it can be deduced that biological organic fertilizer of the invention
There is good control effect to pepper ralstonia solanacearum, reduce the generation of capsicum pest and disease damage, improve Quality of Capsicum.
Claims (10)
1. a kind of biological organic fertilizer of prevention pepper ralstonia solanacearum, which is characterized in that include the raw material of following parts by weight：Luffa 50
~150g, 2~20g of yeast, 10~30g of ferment, 10~30g of activated biomass, 20~50g of N P and K ternary compound fertilizer, non-cause
5~15g of characteristic of disease Ralstonia solanacearum.
2. biological organic fertilizer according to claim 1, which is characterized in that the luffa is magnetic luffa.
3. biological organic fertilizer according to claim 1, which is characterized in that the activated biomass is humus, yolk, six
At least two in ring stone.
4. biological organic fertilizer according to claim 1, which is characterized in that the N P and K ternary compound fertilizer by plant ash,
Calcium superphosphate, urea mix.
5. biological organic fertilizer according to claim 4, which is characterized in that plant ash, calcium superphosphate, urea adding proportion
(by mass) it is 3~5：1：5.
6. a kind of preparation method of biological organic fertilizer as described in claim 1, which is characterized in that include the following steps：
S1, by ripe loofah peeling, remove seed, cut both ends narrower portion, it is 0.5~2cm's or so that remainder, which is cut into thickness,
Column；
S2, daqu fermentation liquid, shaken cultivation is added in luffa；
S3, ferment zymotic fluid, non-pathogenic Ralstonia solanacearum zymotic fluid are uniformly sprayed onto in step S2 load there is yeast micro-
On the luffa of biology, activated biomass is added and is uniformly mixed, adjusts water content 50~70%, compost fermentation；
S4, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S5, by moisture evaporation to water content be 30~40% when, be granulated, pack, quality inspection, certified products sealing storage.
7. the preparation method of biological organic fertilizer according to claim 6, which is characterized in that the luffa is magnetic sponge gourd
Network, preparation method are：Deionized water 100g, Fe is added in the reactor3O4 0.2g、MgSO4 0.15g、MnSO4 0.15g、
CoCl2 0.2g、ZnSO40.2g, ultrasonic disperse are added luffa, are ultrasonically treated to mixed solution, then at suspension
Filtering, drying, obtain magnetic luffa.
8. the preparation method of biological organic fertilizer according to claim 6, which is characterized in that the step S2 shaken cultivations
Condition is：25~37 DEG C, 40~100rpm, 48~72h of shaken cultivation.
9. the preparation method of biological organic fertilizer according to claim 6, which is characterized in that the step S3 compost fermentations
Condition is：First aerobic fermentation 2d, 20~30d of subsequent decomposed fermentation, during which every 2d turnings are primary.
10. a kind of application of such as Claims 1 to 5 any one of them biological organic fertilizer, which is characterized in that the biological organic
Fertilizer is for inhibiting pepper ralstonia solanacearum.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117510281A (en) * | 2023-11-03 | 2024-02-06 | 成都市四友生物科技有限公司 | Organic fertilizer based on chitosan oligosaccharide and preparation method thereof |
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| CN101805716A (en) * | 2010-04-02 | 2010-08-18 | 南京农业大学 | Antagonistic bacterium for controlling bacterial wilt of capsicum and microorganism organic fertilizer thereof |
| CN103641631A (en) * | 2013-12-17 | 2014-03-19 | 湛江师范学院 | Broad-spectrum antibacterial biological fertilizer |
| CN103704069A (en) * | 2013-12-11 | 2014-04-09 | 南京农业大学 | Method of three-dimensionally preventing and controlling tomato bacterial wilt |
| CN105309082A (en) * | 2015-10-21 | 2016-02-10 | 贵州省烟草科学研究院 | Comprehensive preventing and controlling method for tobacco bacterial wilt |
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|---|---|---|---|---|
| CN101805716A (en) * | 2010-04-02 | 2010-08-18 | 南京农业大学 | Antagonistic bacterium for controlling bacterial wilt of capsicum and microorganism organic fertilizer thereof |
| CN103704069A (en) * | 2013-12-11 | 2014-04-09 | 南京农业大学 | Method of three-dimensionally preventing and controlling tomato bacterial wilt |
| CN103641631A (en) * | 2013-12-17 | 2014-03-19 | 湛江师范学院 | Broad-spectrum antibacterial biological fertilizer |
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