CN108578541A - A kind of Chinese medical extract that treating rheumatism and cardiovascular and cerebrovascular disease, preparation method and applications - Google Patents
A kind of Chinese medical extract that treating rheumatism and cardiovascular and cerebrovascular disease, preparation method and applications Download PDFInfo
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Abstract
Rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract, preparation method and applications are treated the present invention provides a kind of.Each raw material relative weight ratio is bark of ash：Rhizoma Chuanxiong：Peach kernel：Safflower：Radix Glycyrrhizae：Rhizoma Et Radix Notopterygii：Myrrh：Radix Angelicae Sinensis：Excrementum pteropi (vinegar-fried)：Rhizoma cyperi：The root of bidentate achyranthes：Earthworm=1：2：3：3：2：1：2：3：2：1：3：2.Preparation method is respectively：1) water extraction method；2) alcohol extracting is followed the example of；3) water extraction and alcohol precipitation method；4) water extraction combines macroporous resin purification method；5) alcohol extracting combination macroporous resin purification method；6) classical decoction preparation method (classics recipe matter basis).The process optimization of object is extracted to the party, is prepared into all kinds of preparations of Chinese medicine, and carries out defining for active part main component.The present invention is detected the product of preparation, the Active regenerator clear and definite of Chinese medical extract, and content is higher.
Description
Technical field
The present invention relates to " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract, preparation method and applications, belong to traditional Chinese medicine technology neck
Domain.
Background technology
Impediment syndrome is the impatency channels and collaterals since the exopathogens such as wind, cold, wet, heat invade human body, and qi and blood is unsmooth caused, with
Pain occurs for muscle, muscles and bones, joint, numb, severe one, joint stuffiness, even scorching hot wait of arthrocele is main clinical manifestation
Disease.《General Treatise on the Cause and Symptoms of Diseases wandering arthritis is waited》It says：" numbness person, wet three gas of chill are miscellaneous extremely, close and form numbness ".
Rheumatism is Traditional Chinese Medical Concepts, is equivalent to the chronic auto-immune systemic disease of modern medicine, the rheumatic of modern medicine
The connective tissue diseases such as arthritis, rheumatoid arthritis, osteoproliferation, spondylitis, osteoarthritis belong to rheumatism scope, master
It is redness and swelling of joints heat pain to want clinical manifestation, and swelling deformation, involving in one or more joints or even articular cavity has liquid exudation,
Function limitation (morning stiffness), some causes life-long disabilities, and it is a kind of not dead cancer to be commonly called as rheumatism.
Rheumatic Bi syndrome is a kind of common refractory disease, and clinical manifestation most outstanding is pain.How effectively to alleviate
Pain is to treat a problem of arthralgia pain due to rheumatism.Doctor trained in Western medicine thinks that this pain belongs to complex regional pain syndrome (CRPS)
One kind, partly cause are to form chronic long-term local inflammation by vasodilation, swelling and oedema to cause relevant cell factor
Local reaction caused by release, doctor trained in Western medicine, which mostly uses, takes glucocorticoid or non-steroidal anti-inflammatory painkiller carrys out symptomatic treatment, controls
Therapeutic effect is very unsatisfactory, often palliative, attends to one thing and lose sight of another, and can only postpone the symptom of patient, and brings alimentary canal
Many side effects such as ulcer, osteoporosis, Cushing syndrome.Chinese medicine generally use dialectical treatmert, achieves certain curative effect, controls
The medicines for focusing on the promoting flow of qi and blood circulation in treatment, to reach gas row then blood, gas row then remove then rheumatism and be cured by wet row, damp evil more.
Traditional Chinese medicine decoction for treating-ache and expelling atagnate derives from Qing Dynasty's Wang Qingren《The correction of the errors of medical works》, " all shoulder pains, brachialgia, pain in the back, skelagia, or the whole body are ached
Bitterly, total name says rheumatism.Know perfectly well and have a cold, is not cured with warm diverging medicine；Know perfectly well have it is damp and hot, it is idle with dampness removing fall fire medicine.Flesh long
Meat is become thin, and yin deficiency is discussed, and is not imitated again with drugs for nourishing yin.So far just cloud：Disease is easy in Pi Mai as work(；Disease is shown in muscles and bones, real difficulty
Effect.Because do not think chill it is damp and hot enter skin, where is had a pain.Enter tracheae, must flow away bitterly；Enter blood vessel, pain does not move place.Such as discuss void
It is weak, it is that void is caused because of disease, it is non-to cause a disease because of void.... ancient prescription is a lot of, if what ancient prescription was controlled does not imitate, uses Traditional Chinese medicine decoction for treating-ache and expelling atagnate." decocting
Clothes.Drug composition is one money of bark of ash, two money of Rhizoma Chuanxiong, three money of peach kernel, three money of safflower, two money of Radix Glycyrrhizae, one money of Rhizoma Et Radix Notopterygii, myrrh two in side
Money, three money of Radix Angelicae Sinensis, two money (stir-fry) of excrementum pteropi (vinegar-fried), one money of rhizoma cyperi, three money of the root of bidentate achyranthes, two money of earthworm (removing soil) cure mainly hemostasis and hold under the arm
Rheumatism, obstruction of meridians and collaterals, shoulder pain, brachialgia, pain in waist and lower extremities, or have aches and pains all over, the person of not recovering for a long time is mainly used for promoting blood circulation, and wind-dispelling removes
It is wet, numbness relieving and pain relieving.Bark of ash, notopterygium wind dispelling dehumidifying, peach kernel, safflower, Radix Angelicae Sinensis, Rhizoma Chuanxiong promoting blood circulation, myrrh, excrementum pteropi (vinegar-fried),
Rhizoma cyperi qi and blood circulation promotion, relieve the pain pain, and the root of bidentate achyranthes, earthworm dredging the channel are with sharp joint, Radix Glycyrrhizae coordinating the drug actions of a prescription.
Traditionally, the preparation process of the prescription and instructions of taking are mainly decocted in water for oral dose：And traditional extraction process by water parameter does not have
Have, it is seen then that traditionally its technique is coarse, and takes, is inconvenient to carry, and dose is big, is also not suitable for being processed into other dosage forms.Have
The modern preparation process of " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " is closed, China Patent No. 02129569.7 discloses《A kind of glue for treating cervical spondylosis
Capsule and preparation method thereof》, but in the patent, medicinal material proportional region is wide, formula is indefinite, and processing step has water extraction,
Steam distillation after water carries and goes ethyl alcohol to stir, has completely disengaged the ingredient that the preparation process of classical ancient prescription is embodied.Technique
Step is very complicated, and only capsule, meanwhile, extract Active regenerator is indefinite.Patent《One kind having stasis-dispelling and pain-killing
Chinese herbal granules of effect and preparation method thereof》In 201310470444.7, the content ratio of reported for work composition
Example is significantly different, and technique uses enzymolysis process, may make certain ingredients that enzymolysis occur like that and even destroy, obtained ingredient with
Our technique acquisition ingredients are entirely different, which has no to limit for active constituent.In addition in novel formulation, new drug, new product
Exploitation and nothing, main active are also blank in research in the embodiment of compound.
Invention content
The purpose of the present invention is overcome the deficiencies of the prior art and provide a kind of to treat rheumatism and cardiovascular and cerebrovascular disease Chinese medicine
Extract, preparation method and applications.System optimization and confirmation are carried out to its main component, solve extract Active regenerator
Unstructured problem.Confirm that pharmacologically curing rheumatism disease is outer, is also suitable on cardiovascular and cerebrovascular disease.
The present invention includes following technical solution：
It is a kind of to treat rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract, with bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Qiang
Work, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm are raw material, and each raw material relative weight ratio is：Bark of ash, Rhizoma Chuanxiong,
Peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：
1：3：2.
In the present invention, bark of ash used, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried),
Rhizoma cyperi, the root of bidentate achyranthes, earthworm raw material are their prepared slices of Chinese crude drugs.
In the present invention, the centival % of involved ethyl alcohol is concentration expressed in percentage by volume %v/v.
Preparation method includes the following steps：Above-mentioned ingredients is cleaned, weighs raw material in proportion, adds 10~15 times of waters
20-30min is impregnated, heating extraction 3 times, first time 2h, second of 1h, third time 30min, each extraction time is after boiling
Start to calculate, each amount of water is identical；Merge extracting solution, extracting solution to the extracting liquid volume for concentrating acquisition is the original
Expect weight 3~5 times obtain initial concentration extracting solution；It is further dried to the Chinese medicine that relative density is 1.0~1.2g/cm3 and carries
Take object.
It can also be following preparation method, include the following steps：Above-mentioned ingredients is cleaned, weighs raw material in proportion,
10~15 times of 65%~85% ethyl alcohol is added to impregnate 20-30min, heating and refluxing extraction 2 times, each 1h, each adds amount of alcohol phase
Together.Merge extracting solution, the volume for concentrating extracting solution to the extracting solution of acquisition is 3~5 times of the raw material weight, is obtained just
Walk concentrated extracting solution；It is 1.0~1.2g/cm3 Chinese medical extracts to be further dried to relative density.
It can also be and 1) prepare concentrate following preparation method：Above-mentioned ingredients is cleaned, weighs raw material in proportion, is added
10~15 times of waters impregnate 20-30min, heating extraction 3 times, first time 2h, second of 1h, third time 30min, each extraction
Time after boiling calculate, each amount of water is identical.Merge extracting solution, concentrates the extracting solution of acquisition to the extracting solution
Volume is 3~5 times of the raw material weight, obtains initial concentration extracting solution；
2) add ethanol precipitation：Add in ethanol in proper amount to the initial concentration extracting solution of above-mentioned preparation, it is 65 to make solvent alcohol content
~75%, 24 hours are stood, filtration, filtrate recycling ethanol obtains refined liquid, concentrates, is drying to obtain Chinese medical extract.
It can also be macroporous resin purification preparation method, include the following steps：
1) concentrate is prepared：Above-mentioned ingredients is cleaned, weighs raw material in proportion, 10~15 times of waters is added to impregnate 20-
30min, heating extraction 3 times, first time 2h, second of 1h, third time 30min, each extraction time after boiling count
It calculates, each amount of water is identical.Merge extracting solution, extracting solution to the extracting liquid volume for concentrating acquisition is the raw material weight
3~5 times, obtain initial concentration extracting solution；
2) macroporous resin purification：Respectively plus in the initial concentration extracting solution that is obtained to upper step of 3~5 times of volume hot water, take
Clear liquid；Supernatant is crossed into macroporous resin column absorption, abandons efflux；Then with the water of 1~5BV with 0.5~2.5BV/ hours stream
Speed washs the adsorption column, abandons water lotion；50~70% ethyl alcohol, 3~5BV of elution volume, with 0.5~2.5BV/ are used later
The flow velocity of hour elutes adsorption column, collects the ethanol eluate, and concentration, the dry ethanol eluate obtain Chinese medical extract.
It can also be another macroporous resin purification preparation method, include the following steps：
1) concentrate is prepared：Each taste prepared slices of Chinese crude drugs are taken, weigh raw material in proportion, add 10~15 times of 65%~85% ethyl alcohol
20-30min is impregnated, heating and refluxing extraction 2 times, each 1h, each adds amount of alcohol identical.Merge extracting solution, concentrates acquisition
The volume of extracting solution to the extracting solution is 3~5 times of the raw material weight, obtains initial concentration extracting solution；
2) macroporous resin purification：Respectively plus in the initial concentration extracting solution that is obtained to step 1) of 3~5 times of volume hot water, take
Supernatant；Supernatant is crossed into macroporous resin column absorption, abandons efflux；Adsorption column described in the water washing of 5BV is first used after loading, abandons water
Washing lotion；Then the adsorption column was washed with 0.5~2.5BV/ hours flow velocitys with the water of 1~5BV, abandons water lotion；It uses later
50~70% ethyl alcohol, 3~5BV of elution volume elute adsorption column with 0.5~2.5BV/ hours flow velocitys, collect the ethyl alcohol and wash
De- liquid, concentration, the dry ethanol eluate, obtains " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract.
In " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract that the above method of the present invention is prepared, amount is with weight
Meter, ferulic acid is not less than 6.0% not less than 0.07%, crocin-I and crocin-II total amounts, using high performance liquid chromatography
Method (HPLC) is measured by ferulic acid, crocin-I and crocin-II total amount contents in the extract that is prepared, with not low
In for reference substance, measurement obtains.
It is a kind of to treat rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract decoction preparation method, which is characterized in that with bark of ash,
Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm are made of raw material, each original
Material relative weight ratio is bark of ash：Rhizoma Chuanxiong：Peach kernel：Safflower：Radix Glycyrrhizae：Rhizoma Et Radix Notopterygii：Myrrh：Radix Angelicae Sinensis：Excrementum pteropi (vinegar-fried)：Rhizoma cyperi：The root of bidentate achyranthes：
Earthworm=1：2：3：3：2：1：2：3：2：1：3：2.
Include the following steps：Each taste prepared slices of Chinese crude drugs are taken, weigh raw material in proportion, add 10 times of waters, heating extraction 2.5h,
The extracting solution that extracting solution concentration obtains obtains initial concentration extracting solution to 5 times that the extracting liquid volume is the raw material weight；
It is further dried to the matter basis for " Traditional Chinese medicine decoction for treating-ache and expelling atagnate " that relative density is 1.0g/cm3.The active ingredient of obtained material benchmark
Ferulic acid is not less than 2.3% not less than 0.03%, crocin-I and crocin-II total amounts.
A kind of oral solid formulation for treating rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract, wherein oral solid formulation
Including：Tablet, hard capsule, soft capsule, granule, oral solution；Granule, hard capsule and additive of tablet be selected from starch, Icing Sugar,
Lactose, dextrin, stevioside, pregelatinized starch, microcrystalline cellulose, calcium bicarbonate, mannitol, hydroxypropyl methyl cellulose, tristearin
The common auxiliary material such as sour magnesium, superfine silica gel powder, talcum powder, polyethylene glycols；Soft capsule auxiliary material is selected from polyethylene glycol (polyethylene glycol
2000-6000), beeswax, atoleine, vegetable oil, glycerine, gelatin etc..Oral solution auxiliary material selects beta-cyclodextrin, sorbierite etc.,
Wherein tablet refers specifically to common compressed tablets, coating tablet, sugar coated tablet, Film coated tablets.
The present invention also provides " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extracts that the above method is prepared to prepare treatment wind
Application in diseases caused by dampness and cardiovascular and cerebrovascular diseases medicament or health products.
The present invention has following advantages：
1, Chinese medical extract of the invention is with bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi
(vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm prepare for raw material.Wherein bark of ash is gentianaceae plant bark of ash (Gentianamacroph μ
LlaPall.), gentiana straminea maxim (GentianastramineaMaxim.), gentiana crassicaulis Duthie (Gentianacras-
SicaulisDuthieexBurk.) or the dry root of bark of ash (GentianadahuricaFisch.), Rhizoma Chuanxiong are samphires
Dry rhizome, the peach kernel of Rhizoma Chuanxiong (Ligusticumchuanxiong.) are rosaceous plant peach (Prunuspersica (L.)
Batsch) or the dry mature seed of mountain peach (Prunusdavidiana (Carr.) Franch.), safflower are feverfew safflower
(CarthamustinctoriusL.) drying flower, radix glycyrrhizae taste are glycyrrhizic legume
(GlycyrrhizauralensisFisch.), glycyrrhiza glabra (GlycyrrhizaglabraL.), swollen fruit Radix
(GlycyrrhizainflataBatal.) root and rhizome, Rhizoma Et Radix Notopterygii are samphire Rhizoma Et Radix Notopterygii (back of the body wing celery)
(NotopterygiumincisumTingexH.T.Chang) or notopterygium forbesii (NotopterygiumforbesiiBoiss.)
Dry rhizome and root, myrrh be Burseraceae myrrh platymiscium myrrh (CommiphoramyrrhaEngl.
[C.molmolEngl.]) and belong to him and plant the oleo-gum-resin of the trunk skin zone exudation of plant, when being classified as umbelliferae angelica
The dry root of (Angelicasinensis (Oliv.) Diels), five excrementum pteropis (vinegar-fried) are flying squirrel section animal trogopterus xanthipes
(TrogopterusXanthipesMilne-Edwards) or the drying of the small flying squirrel of winged murine (PteromysvolansL.)
Excrement,:Vinegar excrementum pteropi method：Net excrementum pteropi is taken, is made popular, vinegar, then fried dry are sprayed.Per excrementum pteropi 100kg, with vinegar 9kg.Rhizoma cyperi is
It is the amaranthaceous plant root of bidentate achyranthes for dry rhizome, the root of bidentate achyranthes of sedge nutgrass flatsedge (CyperusrotundusL.)
(AchyranthesbidentataBlume.) dry root, earthworm are Ju Yin sections animal Pheretima aspergillum
(Pheretimaaspergillum (E.Perrier)), popular Pheretimatschiliensis (PheretimavulgarisChen), William's ring hair
Earthworm (Pheretimaguillelmi (Michaelsen)) or the blind Pheretimatschiliensis of comb
(PheretimapectiniferaMichaelsen) hirudo leech.The Chinese medical extract of acquisition be contained various activity of drug ingredients at
The mixture for dividing collaboration to work, it is different from activated monomer, and drug effect is simple also different from various active ingredients of medicinal materials drug effects
Adduction.
A variety of methods such as 2, present invention employs water to carry, alcohol extracting, water extract-alcohol precipitation, macroporous resin purification, fundamentally ensure
The superiority of " Traditional Chinese medicine decoction for treating-ache and expelling atagnate " side traditional Chinese herbal decoction, the different dosage forms product to be prepared into high quality provide safeguard.The present invention carries
The preparation process of the extract of confession can effectively be enriched with main active such as：Ferulic acid, crocin-I and crocin-II
Total amount etc..
3, quality control carries out assay using high efficient liquid phase analysis method, simple and easy to control.The extract of the present invention
Material base is a certain proportion of multiple active component content compositions, still clear to be put forward for the first time active constituent for the formula
Material base.Embody the novelty of invention.
4, the extract component and ancient prescription preparation process that the various preparation processes of extract according to the present invention are obtained
Compared to (classics recipe matter basis), have active ingredient high, at low cost, process preparation of being more convenient for convenient for marketing and is answered
With.
5, use purifying resin method in macroporous resin purification preparation method provided by the invention, this method is easy, it is quick,
Favorable reproducibility；Selected macroporous resin adsorption amount is big, and desorption efficiency is big, can Reusability, reduce process costs；Solvent used
Ethyl alcohol is inexpensive nontoxic.The extract that preparation method through macroporous resin purification obtains is compared with the decocting object of former prescription, volume
Small, Chinese medicine inherent quality height, product moisture absorption is reduced, stability increases, and is easily formed the various dosage forms of appearance looks elegant.
6, " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract tool treatment rheumatism prepared by preparation method of the invention, rheumatoid and
The functions such as cardiovascular and cerebrovascular disease.And toxicity is low, has good drug safety, and this has fully prompted carrying for this technique preparation
Take the safety of the clinical application of object.
Therefore, heretofore described " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract is suitble to industrialized production, has very strong
Practicability.
Specific implementation mode
The essence of the present invention, but do not limit the invention in any way can be better understood from by the following examples.
Material source：Bark of ash is gentianaceae plant bark of ash (Gentianamacroph μ llaPall.), gentiana straminea maxim
(GentianastramineaMaxim.), gentiana crassicaulis Duthie (Gentianacras-sicaulisDuthieexBurk.) or bark of ash
(GentianadahuricaFisch.) dry root, Rhizoma Chuanxiong are samphire Rhizoma Chuanxiong (Ligusticumchuanxiong.)
Dry rhizome, peach kernel be rosaceous plant peach (Prunuspersica (L.) Batsch) or mountain peach (Prunusdavidiana
(Carr.) Franch.) dry mature seed, safflower be feverfew safflower (CarthamustinctoriusL.) drying
Flower, radix glycyrrhizae taste are glycyrrhizic legume (GlycyrrhizauralensisFisch.), glycyrrhiza glabra
(GlycyrrhizaglabraL.), the root and rhizome of swollen fruit Radix (GlycyrrhizainflataBatal.), Rhizoma Et Radix Notopterygii are umbrella
Shape section plant Rhizoma Et Radix Notopterygii (back of the body wing celery) (NotopterygiumincisumTingexH.T.Chang) or notopterygium forbesii
(NotopterygiumforbesiiBoiss.) dry rhizome and root, myrrh is Burseraceae myrrh platymiscium myrrh
(CommiphoramyrrhaEngl. [C.molmolEngl.]) and belong to he plant plant trunk skin zone exudation oleo-gum-resin,
When be classified as the dry root of umbelliferae angelica (Angelicasinensis (Oliv.) Diels), five excrementum pteropis it is (vinegar-fried) for for
Flying squirrel section animal trogopterus xanthipes (TrogopterusXanthipesMilne-Edwards) or the small flying squirrel of winged murine
(PteromysvolansL.) drying excrement, the drying that rhizoma cyperi is sedge nutgrass flatsedge (CyperusrotundusL.)
Rhizome, the root of bidentate achyranthes are the dry root of the amaranthaceous plant root of bidentate achyranthes (AchyranthesbidentataBlume.), earthworm is that Ju Yin sections are dynamic
Object Pheretima aspergillum (Pheretimaaspergillum (E.Perrier)), popular Pheretimatschiliensis
(PheretimavulgarisChen), power simulation (Pheretimaguillelmi (Michaelsen)) or the blind ring hair of comb
The hirudo leech of earthworm (PheretimapectiniferaMichaelsen).Wherein material is bought by commercial sources.
Embodiment 1 is a kind of to treat rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract preparation method (water extraction method) method 1：
It is raw material to take bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm,
Using water as raw material described in solvent extraction, each raw material weight ratio be bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis,
Excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；Above-mentioned ingredients is cleaned, by than
Example weighs 10 kilograms of bark of ash, 20 kilograms of Rhizoma Chuanxiong, 30 kilograms of peach kernel, 30 kilograms of safflower, 20 kilograms of Radix Glycyrrhizae, 10 kilograms of Rhizoma Et Radix Notopterygii, myrrh
20 kilograms, 30 kilograms of Radix Angelicae Sinensis, 20 kilograms of excrementum pteropi (vinegar-fried), 10 kilograms of rhizoma cyperi, 30 kilograms of the root of bidentate achyranthes, 20 kilograms of earthworm, 2.5 tons of water
Amount impregnates 20min, and 3 times (first time 2h, second of 1h, third time 30min, each extraction time is after boiling for heating extraction
Start to calculate, each amount of water is identical), merge extracting solution, it is described to concentrate extracting solution to the extracting liquid volume of acquisition
3 times of raw material weight obtain initial concentration extracting solution；It is further dried to " the Traditional Chinese medicine decoction for treating-ache and expelling atagnate that relative density is 1.0g/cm3
Side " Chinese medical extract (lot number 18010106).
Method 2：Take bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, ox
Knee, earthworm be raw material, using water as raw material described in solvent extraction, each raw material weight ratio be bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae,
Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；It will be above-mentioned each
Taste medicinal material cleans, weigh in proportion 15 kilograms of bark of ash, 30 kilograms of Rhizoma Chuanxiong, 45 kilograms of peach kernel, 45 kilograms of safflower, 30 kilograms of Radix Glycyrrhizae,
15 kilograms of Rhizoma Et Radix Notopterygii, 30 kilograms of myrrh, 45 kilograms of Radix Angelicae Sinensis, 30 kilograms of excrementum pteropi (vinegar-fried), 15 kilograms of rhizoma cyperi, 45 kilograms of the root of bidentate achyranthes,
30 kilograms of dragon, 3.75 tons of waters impregnate 30min, heating extraction 3 times (first time 2h, second of 1h, third time 30min, each
Extraction time after boiling calculate, each amount of water is identical), merge extracting solution, concentrate the extracting solution of acquisition to described
Extracting liquid volume is 5 times of the raw material weight, obtains initial concentration extracting solution；It is 1.2g/ to be further dried to relative density
" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract (lot number 18010208) of cm3.
Method 3：Take bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, ox
Knee, earthworm be raw material, using water as raw material described in solvent extraction, each raw material weight ratio be bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae,
Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；It will be above-mentioned each
Taste medicinal material cleans, weigh in proportion 13 kilograms of bark of ash, 26 kilograms of Rhizoma Chuanxiong, 39 kilograms of peach kernel, 39 kilograms of safflower, 26 kilograms of Radix Glycyrrhizae,
13 kilograms of Rhizoma Et Radix Notopterygii, 26 kilograms of myrrh, 39 kilograms of Radix Angelicae Sinensis, 26 kilograms of excrementum pteropi (vinegar-fried), 13 kilograms of rhizoma cyperi, 39 kilograms of the root of bidentate achyranthes,
26 kilograms of dragon, 3.25 tons of waters impregnate 25min, heating extraction 3 times (first time 2h, second of 1h, third time 30min, each
Extraction time after boiling calculate, each amount of water is identical), merge extracting solution, concentrate the extracting solution of acquisition to described
Extracting liquid volume is 4 times of the raw material weight, obtains initial concentration extracting solution；It is 1.1g/ to be further dried to relative density
" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract (lot number 18010309) of cm3.
Embodiment 2 is a kind of to treat rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract (alcohol extracting method)
Method 1：Take bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, ox
Knee, earthworm be raw material, using 65% ethyl alcohol as raw material described in solvent extraction, each raw material weight ratio be bark of ash, Rhizoma Chuanxiong, peach kernel, safflower,
Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；It will be upper
It states ingredients to clean, it is public to weigh 10 kilograms of bark of ash, 20 kilograms of Rhizoma Chuanxiong, 30 kilograms of peach kernel, 30 kilograms of safflower, Radix Glycyrrhizae 20 in proportion
Jin, 10 kilograms of Rhizoma Et Radix Notopterygii, 20 kilograms of myrrh, 30 kilograms of Radix Angelicae Sinensis, 20 kilograms of excrementum pteropi (vinegar-fried), 10 kilograms of rhizoma cyperi, 30 kilograms of the root of bidentate achyranthes,
20 kilograms of earthworm, 2.5 ton of 65% ethyl alcohol impregnate 20min, heating and refluxing extraction 2 times, each 1h (each reflux extracting time from
Start to calculate after boiling, each adds amount of alcohol identical), extracting solution merges, and stands 24 hours, supernatant is filtered to take, by supernatant
Liquid is concentrated to 3 times of crude drug, obtains initial concentration extracting solution；It is further dried to " the bodily pain that relative density is 1.0g/cm3
By stasis of blood soup side " Chinese medical extract (lot number 18020423).
Method 2：Take bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, ox
Knee, earthworm be raw material, using 85% ethyl alcohol as raw material described in solvent extraction, each raw material weight ratio be bark of ash, Rhizoma Chuanxiong, peach kernel, safflower,
Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；It will be upper
It states ingredients to clean, it is public to weigh 15 kilograms of bark of ash, 30 kilograms of Rhizoma Chuanxiong, 45 kilograms of peach kernel, 45 kilograms of safflower, Radix Glycyrrhizae 30 in proportion
Jin, 15 kilograms of Rhizoma Et Radix Notopterygii, 30 kilograms of myrrh, 45 kilograms of Radix Angelicae Sinensis, 30 kilograms of excrementum pteropi (vinegar-fried), 15 kilograms of rhizoma cyperi, 45 kilograms of the root of bidentate achyranthes,
30 kilograms of earthworm, 3.75 ton of 85% ethyl alcohol impregnate 30min, heating and refluxing extraction 2 times, each 1h (each reflux extracting times
Calculated after boiling, each adds amount of alcohol identical), extracting solution merges, and stands 24 hours, filters to take supernatant, will be upper
Clear liquid is concentrated to 5 times of crude drug, obtains initial concentration extracting solution；It is further dried to " the body that relative density is 1.2g/cm3
Pain is by stasis of blood soup side " Chinese medicine alcohol extracting thing (lot number 18020524).
Method 3：Take bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, ox
Knee, earthworm be raw material, using 75% ethyl alcohol as raw material described in solvent extraction, each raw material weight ratio be bark of ash, Rhizoma Chuanxiong, peach kernel, safflower,
Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；It will be upper
It states ingredients to clean, it is public to weigh 13 kilograms of bark of ash, 26 kilograms of Rhizoma Chuanxiong, 39 kilograms of peach kernel, 39 kilograms of safflower, Radix Glycyrrhizae 26 in proportion
Jin, 13 kilograms of Rhizoma Et Radix Notopterygii, 26 kilograms of myrrh, 39 kilograms of Radix Angelicae Sinensis, 26 kilograms of excrementum pteropi (vinegar-fried), 13 kilograms of rhizoma cyperi, 39 kilograms of the root of bidentate achyranthes,
26 kilograms of earthworm, 3.25 ton of 75% ethyl alcohol impregnate 25min, heating and refluxing extraction 2 times, each 1h (each reflux extracting times
Calculated after boiling, each adds amount of alcohol identical), extracting solution merges, and stands 24 hours, filters to take supernatant, will be upper
Clear liquid is concentrated to 4 times of crude drug, obtains initial concentration extracting solution；It is further dried to " the body that relative density is 1.1g/cm3
Pain is by stasis of blood soup side " Chinese medicine alcohol extracting thing (lot number 18020625).
Embodiment 3 is a kind of to treat rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract (water extract-alcohol precipitation method)
Method 1：Take bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, ox
Knee, earthworm be raw material, using water as raw material described in solvent extraction, each raw material weight ratio be bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae,
Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；It will be above-mentioned each
Taste medicinal material cleans, weigh in proportion 10 kilograms of bark of ash, 20 kilograms of Rhizoma Chuanxiong, 30 kilograms of peach kernel, 30 kilograms of safflower, 20 kilograms of Radix Glycyrrhizae,
10 kilograms of Rhizoma Et Radix Notopterygii, 20 kilograms of myrrh, 30 kilograms of Radix Angelicae Sinensis, 20 kilograms of excrementum pteropi (vinegar-fried), 10 kilograms of rhizoma cyperi, 30 kilograms of the root of bidentate achyranthes,
20 kilograms of dragon adds 2.5 tons of waters to impregnate 20min, and heating extraction 3 times (first time 2h, second of 1h, third time 30min, every time
Extraction time after boiling calculate, each amount of water is identical), merge extracting solution, concentrate the extracting solution of acquisition to institute
3 times that extracting liquid volume is the raw material weight are stated, initial concentration extracting solution is obtained；It is slowly added to ethanol in proper amount while stirring,
It is 65% to make solvent alcohol content, stands 24 hours, and filtration, filtrate recycling ethanol obtains refined liquid, is concentrated, dry to obtain " bodily pain
By stasis of blood soup side " Chinese medical extract (lot number 18030518).Method 2：It takes bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, have
Medicine, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm are raw material, using water as raw material described in solvent extraction, each raw material weight ratio
For bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：
3：2：1：2：3：2：1：3：2；Above-mentioned ingredients is cleaned, it is public to weigh 15 kilograms of bark of ash, 30 kilograms of Rhizoma Chuanxiong, peach kernel 45 in proportion
Jin, 45 kilograms of safflower, 30 kilograms of Radix Glycyrrhizae, 15 kilograms of Rhizoma Et Radix Notopterygii, 30 kilograms of myrrh, 45 kilograms of Radix Angelicae Sinensis, 30 kilograms of excrementum pteropi (vinegar-fried),
15 kilograms of rhizoma cyperi, 45 kilograms of the root of bidentate achyranthes, 30 kilograms of earthworm add 3.75 tons of waters to impregnate 30min, heating extraction 3 times (first time 2h,
Second of 1h, third time 30min, each extraction time after boiling calculate, each amount of water is identical), merging carries
Liquid is taken, the extracting solution of acquisition is concentrated to 5 times that the extracting liquid volume is the raw material weight, obtains initial concentration extracting solution；
It is slowly added to ethanol in proper amount while stirring, it is 75% to make solvent alcohol content, stands 24 hours, and filtration, filtrate recycling ethanol obtains
Refined liquid, concentration are dry to obtain " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract (lot number 18030620).Method 3：Take bark of ash, river
Rhizome of chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm are raw material, using water as solvent
The raw material is extracted, each raw material weight ratio is bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar
Fry), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；Above-mentioned ingredients is cleaned, weighs bark of ash in proportion
13 kilograms, 26 kilograms of Rhizoma Chuanxiong, 39 kilograms of peach kernel, 39 kilograms of safflower, 26 kilograms of Radix Glycyrrhizae, 13 kilograms of Rhizoma Et Radix Notopterygii, 26 kilograms of myrrh, Radix Angelicae Sinensis
39 kilograms, 26 kilograms of excrementum pteropi (vinegar-fried), 13 kilograms of rhizoma cyperi, 39 kilograms of the root of bidentate achyranthes, 26 kilograms of earthworm, add 3.25 tons of waters to impregnate
25min, heating extraction 3 times (first time 2h, second of 1h, third time 30min, each extraction time after boiling count
Calculate, each amount of water is identical), merge extracting solution, extracting solution to the extracting liquid volume for concentrating acquisition is the raw material weight
4 times of amount obtain initial concentration extracting solution；It is slowly added to ethanol in proper amount while stirring, it is 70% to make solvent alcohol content, stands 24
Hour, filtration, filtrate recycling ethanol obtains refined liquid, concentrates, dry to obtain " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract (lot number
For 18030722).
Embodiment 4 is a kind of to treat rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract (macroporous resin method)
Method 1：Take bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, ox
Knee, earthworm be raw material, using water as raw material described in solvent extraction, each raw material weight ratio be bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae,
Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；It will be above-mentioned each
Taste medicinal material cleans, weigh in proportion 10 kilograms of bark of ash, 20 kilograms of Rhizoma Chuanxiong, 30 kilograms of peach kernel, 30 kilograms of safflower, 20 kilograms of Radix Glycyrrhizae,
10 kilograms of Rhizoma Et Radix Notopterygii, 20 kilograms of myrrh, 30 kilograms of Radix Angelicae Sinensis, 20 kilograms of excrementum pteropi (vinegar-fried), 10 kilograms of rhizoma cyperi, 30 kilograms of the root of bidentate achyranthes,
20 kilograms of dragon, 2.5 tons of waters impregnate 20min, heating extraction 3 times (first time 2h, second of 1h, third time 30min, each
Extraction time after boiling calculate, each amount of water is identical), merge extracting solution, concentrate the extracting solution of acquisition to described
Extracting liquid volume is 3 times of the raw material weight, obtains initial concentration extracting solution；Add 3 times of hot water dilute initial concentration extracting solution
It releases, appropriate Chinese medicine fining agent chitosan is added, centrifuge, go to precipitate, take supernatant, be splined on D101 macroporous resin columns (in loading
Preceding to use acid respectively, alkali, water, alcohol carries out pre-treatment to resin column), first use after loading 1BV pure water rinsings, aqueous solution reject to use instead
50% ethanol elution of 3BV, eluent flow rate 0.5BV/h collect the ethanol eluate.Continue that solvent is recovered under reduced pressure, collects
Medicinal extract, it is dry to obtain " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract (lot number 18040817).
Method 2：Take bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, ox
Knee, earthworm be raw material, using water as raw material described in solvent extraction, each raw material weight ratio be bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae,
Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；It will be above-mentioned each
Taste medicinal material cleans, weigh in proportion 15 kilograms of bark of ash, 30 kilograms of Rhizoma Chuanxiong, 45 kilograms of peach kernel, 45 kilograms of safflower, 30 kilograms of Radix Glycyrrhizae,
15 kilograms of Rhizoma Et Radix Notopterygii, 30 kilograms of myrrh, 45 kilograms of Radix Angelicae Sinensis, 30 kilograms of excrementum pteropi (vinegar-fried), 15 kilograms of rhizoma cyperi, 45 kilograms of the root of bidentate achyranthes,
30 kilograms of dragon, 3.75 tons of waters impregnate 30min, heating extraction 3 times (first time 2h, second of 1h, third time 30min, each
Extraction time after boiling calculate, each amount of water is identical), merge extracting solution, concentrate the extracting solution of acquisition to described
Extracting liquid volume is 5 times of the raw material weight, obtains initial concentration extracting solution；Add 5 times of hot water dilute initial concentration extracting solution
It releases, appropriate Chinese medicine fining agent chitosan is added, centrifuge, go to precipitate, take supernatant, be splined on D101 macroporous resin columns (in loading
Preceding to use acid respectively, alkali, water, alcohol carries out pre-treatment to resin column), first use after loading 5BV pure water rinsings, aqueous solution reject to use instead
70% ethanol elution of 5BV, eluent flow rate 2.5BV/h collect the ethanol eluate.Continue that solvent is recovered under reduced pressure, collects
Medicinal extract, it is dry to obtain " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract (lot number 18040927).
Method 3：Take bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, ox
Knee, earthworm be raw material, using water as raw material described in solvent extraction, each raw material weight ratio be bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae,
Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；It will be above-mentioned each
Taste medicinal material cleans, weigh in proportion 13 kilograms of bark of ash, 26 kilograms of Rhizoma Chuanxiong, 39 kilograms of peach kernel, 39 kilograms of safflower, 26 kilograms of Radix Glycyrrhizae,
13 kilograms of Rhizoma Et Radix Notopterygii, 26 kilograms of myrrh, 39 kilograms of Radix Angelicae Sinensis, 26 kilograms of excrementum pteropi (vinegar-fried), 13 kilograms of rhizoma cyperi, 39 kilograms of the root of bidentate achyranthes,
26 kilograms of dragon, 3.25 tons of waters impregnate 25min, heating extraction 3 times (first time 2h, second of 1h, third time 30min, each
Extraction time after boiling calculate, each amount of water is identical), merge extracting solution, concentrate the extracting solution of acquisition to described
Extracting liquid volume is 4 times of the raw material weight, obtains initial concentration extracting solution；Add 4 times of hot water dilute initial concentration extracting solution
It releases, appropriate Chinese medicine fining agent chitosan is added, centrifuge, go to precipitate, take supernatant, be splined on D101 macroporous resin columns (in loading
Preceding to use acid respectively, alkali, water, alcohol carries out pre-treatment to resin column), first use after loading 2.5BV pure water rinsings, aqueous solution reject to change
With 60% ethanol elution of 4BV, eluent flow rate 1.5BV/h collects the ethanol eluate.Continue that solvent is recovered under reduced pressure, receives
Collect medicinal extract, it is dry to obtain " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract (lot number 18041021).
Embodiment 5 is a kind of to treat rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract (macroporous resin method)
Method 1：Take bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, ox
Knee, earthworm be raw material, using 65% ethyl alcohol as raw material described in solvent extraction, each raw material weight ratio be bark of ash, Rhizoma Chuanxiong, peach kernel, safflower,
Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；It will be upper
It states ingredients to clean, it is public to weigh 10 kilograms of bark of ash, 20 kilograms of Rhizoma Chuanxiong, 30 kilograms of peach kernel, 30 kilograms of safflower, Radix Glycyrrhizae 20 in proportion
Jin, 10 kilograms of Rhizoma Et Radix Notopterygii, 20 kilograms of myrrh, 30 kilograms of Radix Angelicae Sinensis, 20 kilograms of excrementum pteropi (vinegar-fried), 10 kilograms of rhizoma cyperi, 30 kilograms of the root of bidentate achyranthes,
20 kilograms of earthworm, 2.5 ton of 65% ethyl alcohol impregnate 20min, heating and refluxing extraction 2 times, each 1h (each reflux extracting time from
Start to calculate after boiling, each adds amount of alcohol identical), extracting solution merges, and stands 24 hours, supernatant is filtered to take, by supernatant
Liquid is concentrated to 3 times of crude drug, obtains initial concentration extracting solution；By concentrated extracting solution plus 3 times of hot water dilutions, appropriate Chinese medicine is added
Fining agent chitosan, centrifugation, go to precipitate, take supernatant, be splined on D101 macroporous resin columns (before loading respectively use acid, alkali,
Water, alcohol carry out pre-treatment to resin column), it first uses after loading 5BV water (being 5 with 1% hydrochloric acid adjusting PH) to wash the adsorption column, abandons
Water lotion；It is eluted again with neutrality 1BV aqueous solutions, aqueous solution reject uses 50% ethanol elution of 3BV instead, and elution flow rate is
0.5BV/h collects the ethanol eluate.Continue that solvent is recovered under reduced pressure, collects medicinal extract, it is dry to obtain " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medicine
Extract (lot number 18051103).
Method 2：Take bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, ox
Knee, earthworm be raw material, using 85% ethyl alcohol as raw material described in solvent extraction, each raw material weight ratio be bark of ash, Rhizoma Chuanxiong, peach kernel, safflower,
Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；It will be upper
It states ingredients to clean, it is public to weigh 15 kilograms of bark of ash, 30 kilograms of Rhizoma Chuanxiong, 45 kilograms of peach kernel, 45 kilograms of safflower, Radix Glycyrrhizae 30 in proportion
Jin, 15 kilograms of Rhizoma Et Radix Notopterygii, 30 kilograms of myrrh, 45 kilograms of Radix Angelicae Sinensis, 30 kilograms of excrementum pteropi (vinegar-fried), 15 kilograms of rhizoma cyperi, 45 kilograms of the root of bidentate achyranthes,
30 kilograms of earthworm, 3.75 ton of 85% ethyl alcohol impregnate 30min, heating and refluxing extraction 2 times, each 1h (each reflux extracting times
Calculated after boiling, each adds amount of alcohol identical), extracting solution merges, and stands 24 hours, filters to take supernatant, will be upper
Clear liquid is concentrated to 5 times of crude drug, obtains initial concentration extracting solution；By concentrated extracting solution plus 5 times of hot water dilutions, in being added in right amount
Medicine fining agent chitosan, centrifugation, go to precipitate, take supernatant, be splined on D101 macroporous resin columns (before loading respectively use acid, alkali,
Water, alcohol carry out pre-treatment to resin column), it first uses after loading 5BV water (being 6 with 1% hydrochloric acid adjusting PH) to wash the adsorption column, abandons
Water lotion；It is eluted again with neutrality 5BV aqueous solutions, aqueous solution reject uses 70% ethanol elution for washing 5BV instead, and elution flow rate is
2.5BV/h collects the ethanol eluate.Continue that solvent is recovered under reduced pressure, collects medicinal extract, it is dry to obtain " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medicine
Extract (lot number 18051205).
Method 3：Take bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, ox
Knee, earthworm be raw material, using 75% ethyl alcohol as raw material described in solvent extraction, each raw material weight ratio be bark of ash, Rhizoma Chuanxiong, peach kernel, safflower,
Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；It will be upper
It states ingredients to clean, it is public to weigh 13 kilograms of bark of ash, 26 kilograms of Rhizoma Chuanxiong, 39 kilograms of peach kernel, 39 kilograms of safflower, Radix Glycyrrhizae 26 in proportion
Jin, 13 kilograms of Rhizoma Et Radix Notopterygii, 26 kilograms of myrrh, 39 kilograms of Radix Angelicae Sinensis, 26 kilograms of excrementum pteropi (vinegar-fried), 13 kilograms of rhizoma cyperi, 39 kilograms of the root of bidentate achyranthes,
26 kilograms of earthworm, 3.25 ton of 75% ethyl alcohol impregnate 25min, heating and refluxing extraction 2 times, each 1h (each reflux extracting times
Calculated after boiling, each adds amount of alcohol identical), extracting solution merges, and stands 24 hours, filters to take supernatant, will be upper
Clear liquid is concentrated to 4 times of crude drug, obtains initial concentration extracting solution；By concentrated extracting solution plus 5 times of hot water dilutions, in being added in right amount
Medicine fining agent chitosan, centrifugation, go to precipitate, take supernatant, be splined on D101 macroporous resin columns (before loading respectively use acid, alkali,
Water, alcohol carry out pre-treatment to resin column), first use after loading 5BV water (being 5.5 with 1% hydrochloric acid adjusting PH) to wash the adsorption column,
Abandon water lotion；It is eluted again with neutrality 3BV aqueous solutions, aqueous solution reject uses 60% ethanol elution for washing 4BV instead, and elution flow rate is
1.5BV/h collects the ethanol eluate.Continue that solvent is recovered under reduced pressure, collects medicinal extract, it is dry to obtain " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medicine
Extract (lot number 18051228).
6 water extracting method of embodiment prepares " a kind of to treat rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract " Chinese medicine soup
(classics recipe matter basis)
Take bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm
For raw material, using water as raw material described in solvent extraction, each raw material weight ratio is bark of ash, Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, does not have
Medicine, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm=1：2：3：3：2：1：2：3：2：1：3：2；Above-mentioned ingredients is net
It is public to weigh 10 kilograms of bark of ash, 20 kilograms of Rhizoma Chuanxiong, 30 kilograms of peach kernel, 30 kilograms of safflower, 20 kilograms of Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii 10 in proportion for choosing
Jin, 20 kilograms of myrrh, 30 kilograms of Radix Angelicae Sinensis, 20 kilograms of excrementum pteropi (vinegar-fried), 10 kilograms of rhizoma cyperi, 30 kilograms of the root of bidentate achyranthes, 20 kilograms of earthworm,
2.5 tons of water heating extraction 2h, the extracting solution that extracting solution concentration obtains to 5 that the extracting liquid volume is the raw material weight
Times, obtain initial concentration extracting solution；It is further dried to the substance base for " the Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " that relative density is 1.0g/cm3
Accurate (lot number 18011118)
A kind of oral preparation preparation method that treating rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract of embodiment 7
1) preparation prescription of " a kind of to treat rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract " Chinese medical extract granule：
(" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract is taken respectively from embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5, embodiment
6 " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract)
" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract：500.0g
Starch：200.0g
Calcium monohydrogen phosphate：150.0g
Dextrin：100.0g
Lactose：50.0g
Recipe quantity " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract is weighed, the auxiliary material of recipe quantity is added, adds 60% ethyl alcohol softwood, crosses 20
Mesh is pelletized, 50~60 DEG C of forced air dryings (moisture control is 5%~8%), crosses 16 mesh sieves to get " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side "
Grain.
2) preparation of " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract coating tablet
Prescription：(" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract be taken respectively from embodiment 1, embodiment 2, embodiment 3, embodiment 4,
" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract of embodiment 5, embodiment 6)
" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract：500.0g
Pregelatinized starch：200.0g
Microcrystalline cellulose：230.0g
Stevioside：25.0g
Sucrose：25.0g
Magnesium stearate：10.0g
Superfine silica gel powder：10.0g
Coating agent：Promise coloured silk TM coating agents soluble in the stomach (appropriate)
The auxiliary material of recipe quantity is added (outside magnesium stearate, superfine silica gel powder in " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract for weighing recipe quantity
Add), add 60% ethyl alcohol softwood, cross the granulation of 20 mesh, 50~60 DEG C of forced air dryings (moisture control is 5%~8%) cross 16 mesh sieve
Whole grain is uniformly mixed, tabletting with additional auxiliary material magnesium stearate, superfine silica gel powder, is coated to get " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " coating tablet.
3) preparation of " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract capsule
Prescription：(" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract be taken respectively from embodiment 1, embodiment 2, embodiment 3, embodiment 4,
" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract of embodiment 5, embodiment 6)
" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract：500.0g
Hydroxypropyl methylcellulose：40.0g (the additional half Inner of half adds)
Pregelatinized starch：190.0g
Microcrystalline cellulose：200.0g
Lactose：25.0g
Icing Sugar：25.0g
Magnesium stearate：10.0g
Talcum powder：10.0g
Recipe quantity " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract is weighed, the auxiliary material (magnesium stearate, talcum powder are additional) of recipe quantity is added,
Add 60% ethyl alcohol softwood, cross the granulation of 20 mesh, it is whole to cross 16 mesh sieve for 50~60 DEG C of forced air dryings (moisture control is 5%~8%)
Grain, is uniformly mixed with additional auxiliary material magnesium stearate, superfine silica gel powder, is filled to get " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " glue with filling machine
Capsule.
4) preparation of " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract soft capsule
Prescription：(" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract be taken respectively from embodiment 1, embodiment 2, embodiment 3, embodiment 4,
" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract of embodiment 5, embodiment 6)
" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract：500.0g
Vegetable oil：140.0g
Sorbierite：100.0g
Glycerine：50.0g
Atoleine：60.0g
Gelatin：150.0g
It weighs recipe quantity " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract to be uniformly mixed with the vegetable oil of recipe quantity, sorbierite, by gelatin
It is contained in hopper respectively with glycerine, two liquid are sprayed by the double-deck nozzle of concentric tube-shaped with friction speed, are made a certain amount of
It after glue wraps up a certain amount of liquid, instills in another coolant liquid (atoleine) that do not dissolve each other mutually, glue contact cooling
Because surface tension effects become spherical after liquid, and gradually solidification forms soft capsule.
5) preparation of " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract oral solution
Prescription：(" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract be taken respectively from embodiment 1, embodiment 2, embodiment 3, embodiment 4,
" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract of embodiment 5, embodiment 6)
" Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract：500.0g
Beta-cyclodextrin：140.0g
Sucrose：100.0g
Distilled water 260.0g
Recipe quantity " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract is weighed, the auxiliary material of recipe quantity is added, adds distillation water dissolution, stirring equal
It is even to get " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract oral liquid.
Embodiment 8 " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract and determination of bioactive constituent
1. chromatographic condition
Agilent1260 high performance liquid chromatographs, UV detector (DAD) are to fill out with octadecylsilane chemically bonded silica
Fill agent, C18 chromatographic columns (4.6mm × 250mm, 5 μm)；Ferulic acid (National Institute for Food and Drugs Control, lot number：110773-
20101), Detection wavelength 321nm, mobile phase：- 1% acetum (30 of methanol：70)；Crocin-I (Chinese drug biology systems
Institute, lot number are determined in product examine：110608-201208) and crocin-II (Nat'l Pharmaceutical ＆ Biological Products Control Institute, lot number：110709-
201206) Detection wavelength of total amount：440nm, mobile phase：Methanol-water (45：55)；Flow velocity：1.0ml/min；Column temperature：30℃；Into
Sample amount：10μl.
The preparation of " 2. Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract sample
1) extract of the present invention
Each 1 lot number of above-mentioned Chinese medical extract is made respectively by the method described in present example 1,2,3,4,5,6.
3. the preparation of standard solution
Take ferulic acid reference substance appropriate, it is accurately weighed, it sets in brown measuring bottle, adds 70% methanol that every lml is made and contain 20 μ g's
Solution to get；Take crocin-I reference substances, crocin-II reference substances appropriate, it is accurately weighed, add Diluted Alcohol to be respectively prepared
Per lm respectively contain 30 μ g and 12 μ g solution to get.
4. assay method
Take the above-mentioned Chinese medical extract sample of each lot number appropriate respectively, accurately weighed about 10-25mg is transferred to 50ml capacity
In bottle, add methanol 40ml, ultrasound 30min under room temperature, taking-up is cooled to room temperature, adds methanol to scale, shakes up, and draws appropriate, determines
Hold to 50ml, shake up, you can measures.Each component content is calculated according to one point external standard method.It the results are shown in Table 1：
Table 1 " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " extract and each component assay result
Therefore " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract (embodiment 1-5) and its content are by weight, ferulic acid is not less than
0.07%, crocin-I and crocin-II total amounts are not less than 6.0%." Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " primary standard substance (embodiment 6)
And its content is by weight, ferulic acid is not less than 2.3% not less than 0.03%, crocin-I and crocin-II total amounts.
A kind of anti-inflammatory experimental study that treating rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract of embodiment 9
Kunming mice 170, sub-model group, anti-inflammatory be logical, sample 1-1 (lot numbers：18010106), sample 1-2 (lot numbers：
18010208), sample 1-3 (lot numbers：18010309), sample 2-1 (lot numbers：18020423), sample 2-2 (lot numbers：
18020524), sample 2-3 (lot numbers：18020625), sample 3-1 (lot numbers：18030518), sample 3-2 (lot numbers：
18030620), sample 3-3 (lot numbers：18030722), sample 4-1 (lot numbers：18040817), sample 4-2 (lot numbers：
18040927), sample 4-3 (lot numbers：18041021), sample 5-1 (lot numbers：18051103), sample 5-2 (lot numbers：
18051205), sample 5-3 (lot numbers：18051228) totally ten seven groups.Each 10 of every group of animal, the administration of each group mouse continuous gavage
10 days, inflammation is only caused in mouse right ear coating dimethylbenzene 0.02ml/ after the last administration.Cervical dislocation puts to death mouse after being spaced 3h,
Ears are cut along auricle baseline, auricle is laid at the same position of two ears with diameter 8mm card punch and are weighed.It the results are shown in Table 2.
Compared with model group, positive controls, sample 1-1,1-2,1-3,2-1,2-2,2-3,3-1,3-2,3-3,4-1,
4-2,4-3,5-1,5-2,5-3 have pole significant difference (p to mice ear degree and negative control group<0.05)；Illustrate each sample
Significant anti-inflammatory effect, meanwhile, sample 4-1,4-2,4-3 and 5-1,5-2,5-3 antiphlogistic effects are better than sample 1-1,1-2,1-
3,2-1,2-2,2-3,3-1,3-2,3-3, sample 6-1 have certain antiphlogistic effects.
2 paraxylene of table cause mice ear influence (N=10)
| Group | Dosage (g/kg) | Swelling (mg) |
| Model group | —— | 7.05±2.12 |
| Anti-inflammatory is logical | 2.000 | 3.25±1.45*** |
| Sample 1-1 | 1.400 | 6.49±1.12* |
| Sample 1-2 | 1.400 | 6.46±1.26* |
| Sample 1-3 | 1.400 | 6.42±1.33* |
| Sample 2-1 | 1.100 | 6.62±1.12* |
| Sample 2-2 | 1.100 | 6.58±1.06* |
| Sample 2-2 | 1.100 | 6.66±1.21* |
| Sample 3-1 | 0.900 | 5.01±1.17* |
| Sample 3-2 | 0.900 | 5.08±1.04* |
| Sample 3-3 | 0.900 | 5.09±1.34* |
| Sample 4-1 | 0.530 | 4.39±1.23** |
| Sample 4-2 | 0.530 | 4.32±1.19** |
| Sample 4-3 | 0.530 | 4.35±1.08** |
| Sample 5-1 | 0.450 | 4.12±1.37** |
| Sample 5-2 | 0.450 | 4.17±1.46** |
| Sample 5-3 | 0.450 | 4.19±1.24** |
| Sample 6-1 | 1.400 | 6.75±1.12* |
Compared with model group, * p<0.05；**p<0.01；***p<0.001
A kind of experimental study promoting blood circulation and removing blood stasis that treating rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract of embodiment 10
1 materials and methods
1.1 experimental animal
Healthy cleaning grade male SD rat 180,180~220g of weight are purchased from The Fourth Military Medical University experimental animal
The heart, credit number:SCXK (army) 2012-0007.
1.2 reagents and instrument
Endothelin -1, nitric oxide, 6- ketone-prostaglandin-F1 α, thromboxane B2 kit, by Shanghai western Tang's biology section
Skill Co., Ltd provides；ZL9000C type fully automatic blood viscosity apparatus (Beijing Zhongchi Weiye Science and Technology Development Co., Ltd.).
1.3 drug
Inventive samples (embodiment 1-6).Adrenalin hydrochloride injection, Shanghai Hefeng Pharmaceutical Co., Ltd.'s production,
1mg/mL, lot number:H12020526.
1.4 method
1.4.1 animal model is prepared and is grouped
After adaptable fed 1 week, 180 rats are randomly divided into 18 groups (n=10):Control group, model group, sample 1-1
(lot number：18010106), sample 1-2 (lot numbers：18010208), sample 1-3 (lot numbers：18010309), sample 2-1 (lot numbers：
18020423), sample 2-2 (lot numbers：18020524), sample 2-3 (lot numbers：18020625), sample 3-1 (lot numbers：
18030518), sample 3-2 (lot numbers：18030620), sample 3-3 (lot numbers：18030722), sample 4-1 (lot numbers：
18040817), sample 4-2 (lot numbers：18040927), sample 4-3 (lot numbers：18041021), sample 5-1 (lot numbers：
18051103), sample 5-2 (lot numbers：18051205), sample 5-3 (lot numbers：18051228), sample 6-1 (lot numbers：
18011118).In addition to control group, all rats provide feed according to rat body weight 40g/kg standards daily, and (give half in the morning
Feed gives half feed at night), continuous 27 days；Start within 8th, on the basis of limitation of food intake, is put into (18 ± 2) DEG C water
Went swimming, pulls out when tired and (nature occurs with the rat of every group of half to sink for degree), continuous 20 days.In addition to control group, from
Starting within 18th, on the basis of limit food, swimming, adrenalin hydrochloride injection is subcutaneously injected in every rat before swimming,
0.4mL/kg, totally 10 days.According to the change of the ordinary circumstances such as rat body weight situation, the state of mind, tongue nature, hair and hemorheology
Variation is learned, judges whether modeling succeeds.
1.4.2 medication
According to people and rat body surface area than calculating dosage.Starting within 8th, sample sets give 2.7g/ (kgd),
Gastric infusion, one time a day, continuous 27 days, control group and model group gavaged same volume distilled water.
1.4.3 observation index
After last dose 1 hour, 10% chloraldurate intraperitoneal injection of anesthesia is given, abdominal aortic blood, heparin sodium anti-freezing, from
Supernatant is taken after the heart.The content of ET-1, NO, 6-keto-PGF1 α, TXB2 are measured using Enzyme-linked Immunosorbent Assay method, specific steps are tight
Lattice are operated by kit specification.Hemorheology application fully automatic blood viscosity apparatus is detected according to operating process.
1.5 statistical procedures
Measurement data is with mean ± standard deviationIt indicates, testing result, group is analyzed using SPSS17.0 statistical softwares
Between comparison in difference use one-way analysis of variance, compare examined using LSD-t two-by-two.P<0.05 is statistically significant for difference.
2 results
2.1 each group hemorheology of rat change
Compared with the control group, each index of model group significantly increases, and difference has statistical significance (P<0.01)；With model
Group compares, and sample sets whole blood viscosity and Plasma Viscosity significantly reduce (P<0.05 or P<0.01).Show inventive samples to gas
Empty blood stasis rat model hemorheological property has improvement result, and more notable with the effect of embodiment 1-5 each groups, and embodiment 6 also has
It significantly improves.
2.2 each group rat ET-1, NO changes of contents
Compared with the control group compared with model group ET-1 is horizontal significantly raised, and NO levels significantly reduce, and difference is statistically significant
(P<0.01).Sample sets (embodiment 1-6) ET-1 is horizontal and NO contents are compared with model group, not statistically significant (P>
0.05).Compared with model group, the amount of sample sets (embodiment 1-6) significantly reduces (P<0.01), the amount of NO significantly increases (P<
0.01)。
The changes of contents of 2.3 each group rat TXB2,6-keto-PGF1 α
Compare control group, and model group thromboxane TXB2 is significantly raised, 6-keto-PGF1 alpha levels reduce, 6-keto-
PGF1 α/TXB2 ratios reduce, the statistically significant (P of difference<0.01).Compared with model group, sample sets (embodiment 1-6)
The amount of TXB2 significantly reduces (P<0.05 or P<0.01), 6-keto-PGF1 α apparent increases (P<0.05 or P<0.01)；Sample sets
6-keto-PGF1 α/TXB2 ratios, the statistically significant (P of difference can be improved in (embodiment 1-6)<0.01).
【Embodiment 11】The acute toxicological experiment of " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract
Preliminary experiment：By acute toxicological experiment method, kunming mice 40, fasting is selected to can't help water 12h.Bodily pain by the stasis of blood
Decoction extract is configured to the suspension of maximum concentration, 2.738g/mL containing crude drug (not block irrigation stomach device as limit).Every mouse
Each gavage is about 0.8mL/20g (weight), and interior gavage 3 times, are spaced 4h for 24 hours.Then it routinely raises, and observes the poison of mouse
Property response situation (appearance, activity, breathing, secretion, defecation, death etc.), and continuous weighting 7 days.Because with maximum administration concentration,
Maximum administration capacity, does not measure median lethal dose (LD50), therefore does maximum dosage-feeding experiment.
Maximum dosage-feeding is tested：On the basis of trial test, using maximum dosage-feeding method, Kun Ming mice 20 is selected
Only, half male and half female, weight (20 ± 2) g.Water 12h is can't help in the preceding fasting of administration.And make up a prescription by Pre-testing procedure, with every mouse
Give maximum crude drug concentration 2.738g/mL, the liquid gavage of maximum volume 0.8mL/20g, with the appearance of mouse in observation 14 days,
Situations such as diet, activity, excretion, death.Mouse is put to death with cervical dislocation after 14 days, observed after dissection its important organ (heart,
Liver, spleen, lung, kidney, stomach, intestines etc.) variation.
1) general performance
After administration in 14 days, experimental mice diet is normal, and fur is smooth, and freely, stool and urine is normal, without exception point for activity
Secretion, respiratory system, cardio-cerebrovascular, nervous system, digestive system and urinary system are showed no exception, and mouse is all deposited
It is living.Its daily maximum dosage-feeding is more than 305.5g/kg.
2) changes of weight situation
After administration in 14 days, 2 groups of mouse weights increase, by analyze be administered after 7 days, Chinese medicine group in 14 days 2 times
With the changes of weight of control group, it is found that there was no significant difference (P ＞ 0.05) for 2 groups of weight.
3) main organs are observed
2 groups of mouse are through gross anatomy and observe by the naked eye, and have no the abnormal phenomenon such as internal organs enlargement, atrophy, necrosis, mainly
Internal organs are close with blank control group without any bad lesion.In conclusion prompting this medicine nontoxic, clinical application amount is one
A safe dosage, clinical application safety.
Claims (9)
1. a kind for the treatment of rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract, which is characterized in that with bark of ash, Rhizoma Chuanxiong, peach kernel, red
Flower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm are made of raw material, each raw material relative weight ratio
For bark of ash：Rhizoma Chuanxiong：Peach kernel：Safflower：Radix Glycyrrhizae：Rhizoma Et Radix Notopterygii：Myrrh：Radix Angelicae Sinensis：Excrementum pteropi (vinegar-fried)：Rhizoma cyperi：The root of bidentate achyranthes：Earthworm=1：2：3：
3：2：1：2：3：2：1：3：2；
Preparation method includes the following steps：Each taste prepared slices of Chinese crude drugs are taken, weigh raw material in proportion, 10~15 times of waters is added to impregnate
20-30min, heating extraction 3 times；Each amount of water is identical, extraction time after boiling calculate first time 2h, second
1h, third time 30min merge extracting solution, concentrate the extracting solution of acquisition to 3 that the extracting liquid volume is the raw material weight~
5 times, obtain initial concentration extracting solution；It is 1.0~1.2g/cm to be further dried to relative density3Chinese medical extract.
2. a kind for the treatment of rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract, which is characterized in that with bark of ash, Rhizoma Chuanxiong, peach kernel, red
Flower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm are made of raw material, each raw material relative weight ratio
For bark of ash：Rhizoma Chuanxiong：Peach kernel：Safflower：Radix Glycyrrhizae：Rhizoma Et Radix Notopterygii：Myrrh：Radix Angelicae Sinensis：Excrementum pteropi (vinegar-fried)：Rhizoma cyperi：The root of bidentate achyranthes：Earthworm=1：2：3：
3：2：1：2：3：2：1：3：2；
Preparation method includes the following steps：Each taste prepared slices of Chinese crude drugs are taken, weigh raw material in proportion, add 10~15 times 65%~85%
Ethyl alcohol impregnates 20-30min, and heating and refluxing extraction 2 times, each adds amount of alcohol identical, and each 1h merges extracting solution, and concentration obtains
The volume of the extracting solution obtained to the extracting solution is 3~5 times of the raw material weight, obtains initial concentration extracting solution；Further
Drying to relative density is 1.0~1.2g/cm3Chinese medical extract.
3. a kind for the treatment of rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract, which is characterized in that with bark of ash, Rhizoma Chuanxiong, peach kernel, red
Flower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm are made of raw material, each raw material relative weight ratio
For bark of ash：Rhizoma Chuanxiong：Peach kernel：Safflower：Radix Glycyrrhizae：Rhizoma Et Radix Notopterygii：Myrrh：Radix Angelicae Sinensis：Excrementum pteropi (vinegar-fried)：Rhizoma cyperi：The root of bidentate achyranthes：Earthworm=1：2：3：
3：2：1：2：3：2：1：3：2；
Preparation method includes the following steps：
1) concentrate is prepared：Above-mentioned ingredients is cleaned, weighs raw material in proportion, 10~15 times of waters is added to impregnate 20-
30min, heating extraction 3 times；Each amount of water is identical, extraction time after boiling calculate, first time 2h, second
1h, third time 30min merge extracting solution, concentrate the extracting solution of acquisition to 3 that the extracting liquid volume is the raw material weight~
5 times, obtain initial concentration extracting solution；
2) add ethanol precipitation：Add in ethanol in proper amount to the initial concentration extracting solution of above-mentioned preparation, make solvent alcohol content be 65~
75%, 24 hours are stood, filtration, filtrate recycling ethanol obtains refined liquid, concentrates, is drying to obtain Chinese medical extract.
4. a kind for the treatment of rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract, which is characterized in that with bark of ash, Rhizoma Chuanxiong, peach kernel, red
Flower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm are made of raw material, each raw material relative weight ratio
For bark of ash：Rhizoma Chuanxiong：Peach kernel：Safflower：Radix Glycyrrhizae：Rhizoma Et Radix Notopterygii：Myrrh：Radix Angelicae Sinensis：Excrementum pteropi (vinegar-fried)：Rhizoma cyperi：The root of bidentate achyranthes：Earthworm=1：2：3：
3：2：1：2：3：2：1：3：2；
Preparation method includes the following steps：
1) concentrate is prepared：Above-mentioned ingredients is cleaned, weighs raw material in proportion, 10~15 times of waters is added to impregnate 20-
30min, heating extraction 3 times；Each amount of water is identical, extraction time after boiling calculate, first time 2h, second
1h, third time 30min merge extracting solution, concentrate the extracting solution of acquisition to 3 that the extracting liquid volume is the raw material weight~
5 times, obtain initial concentration extracting solution；
2) macroporous resin purification：Respectively plus in the initial concentration extracting solution that is obtained to upper step of 3~5 times of volume hot water, supernatant is taken；
Supernatant is crossed into macroporous resin column absorption, abandons efflux；Then it is washed with 0.5~2.5BV/ hours flow velocitys with the water of 1~5BV
The adsorption column, abandons water lotion；50~70% ethyl alcohol, 3~5BV of elution volume, with 0.5~2.5BV/ hours are used later
Flow velocity elutes adsorption column, collects the ethanol eluate, and concentration, the dry ethanol eluate obtain Chinese medical extract.
5. a kind for the treatment of rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract, which is characterized in that with bark of ash, Rhizoma Chuanxiong, peach kernel, red
Flower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm are made of raw material, each raw material relative weight ratio
For bark of ash：Rhizoma Chuanxiong：Peach kernel：Safflower：Radix Glycyrrhizae：Rhizoma Et Radix Notopterygii：Myrrh：Radix Angelicae Sinensis：Excrementum pteropi (vinegar-fried)：Rhizoma cyperi：The root of bidentate achyranthes：Earthworm=1：2：3：
3：2：1：2：3：2：1：3：2；
Preparation method includes the following steps：
1) concentrate is prepared：Each taste prepared slices of Chinese crude drugs are taken, weigh raw material in proportion, 10~15 times of 65%~85% ethyl alcohol is added to impregnate
20-30min, heating and refluxing extraction 2 times, each adds amount of alcohol identical, and each 1h merges extracting solution, concentrates the extraction of acquisition
The volume of liquid to the extracting solution is 3~5 times of the raw material weight, obtains initial concentration extracting solution；
2) macroporous resin purification：Respectively plus in the initial concentration extracting solution that is obtained to upper step of 3~5 times of volume hot water, supernatant is taken；
Supernatant is crossed into macroporous resin column absorption, abandons efflux；Adsorption column described in the water washing of 5BV is first used after loading, abandons water lotion；So
The adsorption column was washed with 0.5~2.5BV/ hours flow velocitys with the water of 1~5BV afterwards, abandons water lotion；50~70% are used later
Ethyl alcohol, 3~5BV of elution volume elutes adsorption column with 0.5~2.5BV/ hours flow velocitys, collects the ethanol eluate, dense
Contracting, the dry ethanol eluate, obtain " Traditional Chinese medicine decoction for treating-ache and expelling atagnate side " Chinese medical extract.
6. such as the Chinese medical extract that any one method is prepared in claim 1-6, which is characterized in that with weight ratio meter,
It is not less than 6.0% not less than 0.07%, crocin-I and crocin-II total amounts containing ferulic acid in the extract.
7. a kind of decoction preparation method for treating rheumatism and cardiovascular and cerebrovascular disease Chinese medical extract, which is characterized in that with bark of ash,
Rhizoma Chuanxiong, peach kernel, safflower, Radix Glycyrrhizae, Rhizoma Et Radix Notopterygii, myrrh, Radix Angelicae Sinensis, excrementum pteropi (vinegar-fried), rhizoma cyperi, the root of bidentate achyranthes, earthworm are made of raw material, each original
Material relative weight ratio is bark of ash：Rhizoma Chuanxiong：Peach kernel：Safflower：Radix Glycyrrhizae：Rhizoma Et Radix Notopterygii：Myrrh：Radix Angelicae Sinensis：Excrementum pteropi (vinegar-fried)：Rhizoma cyperi：The root of bidentate achyranthes：
Earthworm=1：2：3：3：2：1：2：3：2：1：3：2；
Preparation method includes the following steps：Each taste prepared slices of Chinese crude drugs are taken, weigh raw material in proportion, add 10 times of waters, heating extraction
2.5h, the extracting solution that extracting solution concentration obtains to 5 times that the extracting liquid volume is the raw material weight, obtains initial concentration and carries
Take liquid；It is 1.0g/cm to be further dried to relative density3Matter basis；The active ingredient ferulic acid of obtained material benchmark
It is not less than 2.3% not less than 0.03%, crocin-I and crocin-II total amounts.
8. containing the pharmaceutical composition that any one method is prepared in such as claim 1-6, which is characterized in that described
Composition is oral preparation, such as oral solution, capsule, soft capsule, pill, granule, and the oral preparation includes pharmacy
Upper acceptable carrier and/or excipient.
9. as any one method is prepared in claim 1-6 Chinese medical extract middle preparations treatment rheumatism (rheumatism,
Rheumatoid) and cardiovascular and cerebrovascular diseases medicament or health products in application.
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Address after: 430000 Yongan East Road, Xianning hi tech Industrial Development Zone, Xianning, Hubei, 63 Applicant after: Hubei Yiren General Institute of traditional Chinese Medicine Co., Ltd Address before: 430000 Yongan East Road, Xianning hi tech Industrial Development Zone, Xianning, Hubei, 63 Applicant before: HAIWANG (HUBEI) CHINESE MEDICINE RESEARCH INSTITUTE Co.,Ltd. |
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