CN108553338A - A kind of anti-inflammatory moist lip protector - Google Patents
A kind of anti-inflammatory moist lip protector Download PDFInfo
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- CN108553338A CN108553338A CN201810430108.2A CN201810430108A CN108553338A CN 108553338 A CN108553338 A CN 108553338A CN 201810430108 A CN201810430108 A CN 201810430108A CN 108553338 A CN108553338 A CN 108553338A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/001—Preparations for care of the lips
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6489—Metalloendopeptidases (3.4.24)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24072—Fibrolase (3.4.24.72)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- General Engineering & Computer Science (AREA)
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- Biochemistry (AREA)
- Dermatology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to cosmetic fields, and in particular to a kind of anti-inflammatory moist lip protector.Including following mass parts substance：62 68 parts of grease, 22 26 parts of wax, 68 parts of natural perfume material, 3.2 4.8 parts of solvent, 14 parts of moisturizer, 68 parts of natural antiseptic agent, 68 parts of keratolytic, 2 parts of vitamin e1,180 200usp/100g of earthworm fibrinolysin.The effect of earthworm fibrinolysin is made lipstick, makes it have anti-oxidant and anti-inflammatory is added in the present invention, effectively lip can be kept to moisten, and improves lip microgroove and dry and cracked phenomenon, prevents angular stomatitis, helps skin of lip sun-proof, safeguards lip health status.
Description
Technical field
The present invention relates to cosmetic fields, and in particular to a kind of anti-inflammatory moist lip protector.
Background technology
Lip is the most thin place of skin, self-protection and the poor ability for preventing external injury.Lip is for a long time by outside
The influence of harmful substance has ignored the protection to lip, can cause lip aging along with present life and work pressure is excessive,
Generate deep layer lines and dry and cracked phenomenon, it is serious may virus infection lead to the diseases such as cold sores.
Earthworm also known as earthworm are a kind of low annelids, have very strong wound-healing abilities.Earthworm type is various,
As medicinal early on the books in China.Modern research shows that contain the ingredients such as lumbricin, enzyme, vitamin in earthworm, tool decompression,
It relievings asthma, is antipyretic, diuresis access, antiallergy, solving the pharmacological actions such as taking poison and growing muscle due, thrombolysis fibrinolytic and anticoagulation.Earthworm fibrinolysin (EFE)
Plasmin activity with direct solution fibrin, and the activity of the Plasminogen activation with class urokinase, therefore can be molten
It solves outmoded thrombus and new thrombosis can be inhibited.It is extracted from earthworm from the U.S. former permanent report of nineteen eighty-three Japan Gong Qi medical university
To the research of active constituent in earthworm unprecedentedly active since fibrinolysin with greater activity, Chinese scholar is also anti-to earthworm
Bolt and thrombolytic effect have carried out a series of experimental study of more systems.There is oral and injection drug for clinical at present,
However it is also less for its research in terms of skin repair.
Technical solution
In view of the above problems, earthworm fibrinolysin, which is added, in the present invention is made lip gloss, anti-oxidant and anti-inflammatory work(is made it have
Effect, effectively can keep lip to moisten, and improve lip microgroove and dry and cracked phenomenon, prevent angular stomatitis, help skin of lip sun-proof, dimension
Lip care portion health status.
For achieving the above object, the present invention takes following technical scheme：
A kind of anti-inflammatory moist lip protector, including following mass parts substance：62-68 parts of grease, 22-26 parts of wax, natural perfume material
6-8 parts, 3.2-4.8 parts of solvent, 1-4 parts of moisturizer, 6-8 parts of natural antiseptic agent, 6-8 parts of keratolytic, -2 parts of vitamin e1,
Earthworm fibrinolysin 180-200usp/100g.
Preferably, including following mass parts substance：It is 64-66 parts of grease, 23-25 parts of wax, 6.5-7.5 parts of natural perfume material, molten
3.6-4.4 parts of agent, 2-3 parts of moisturizer, 6.5-7.5 parts of natural antiseptic agent, 6.5-7.5 parts of keratolytic, vitamin e1 .2-
1.8 parts, earthworm fibrinolysin 185-195usp/100g.
The grease is at least two in mango oil, jojoba seed oil, Sweet Almond Oil, sher butter, tea oil.
The wax is at least two in lanolin wax, cholesterol, cera alba, glyceride, and the acid value of the cera alba is
5.0-8.0。
The natural perfume material is one kind in Rosa Damascana, cherry essence, Lavender, tangerine oil.
The natural antiseptic agent is forsythia suspense extraction, Rosmarinus officinalis extract, thyme extract, extractive of perilla, green tea
At least two in extract.
The moisturizer is at least one of Sodium Hyaluronate, sodium alginate, lactalbumin, casein.
The keratolytic is at least two in evening primrose oil, grape seed oil, cherry nut oil, angle soft-shelled turtle alkane.
The solvent is diisopropyl adipate.
The preparation method of the earthworm fibrinolysin includes the following steps：
A) it chooses wild Pheretimatschiliensis and is rinsed with water surface, be rinsed with water 1h again overnight, dirt is made to be discharged as possible；
B) earthworm after cleaning is inhaled and is twisted into homogenate in high-speed tissue mashing machine, add isometric NH4Ac-HAc
Buffer solution；It is extracted at 4 DEG C overnight, centrifugation takes supernatant；
C) solid (NH is added in supernatant4)2SO4It to 0.6 saturation degree, saltouts at 4 DEG C for 24 hours, centrifugation collects precipitation, thoroughly
Analysis is desalted, and 8000r/min centrifuges 30min, and supernatant is crude enzyme liquid；
D) 1 is pressed：Phosphoric acid flushing liquor (PBS) is added in 2 ratio, and homogenate is placed in refrigerator and extracts 6h, centrifuging and taking supernatant；
E) by thick enzyme solutions by DEAE-SepharoseCL-6B ion exchanges, Sephadex G-75 gel chromatographies are received
It is pure enzyme solutions to collect active part.
Beneficial effects of the present invention are：
1. the present invention is added earthworm fibrinolysin and lip gloss is made, there is the effect of anti-oxidant anti-inflammatory, it is dry that lip can be prevented
It splits, angular stomatitis, protection skin of lip is sun-proof, and long-time service can delay labial aging, improves skin of lip, and lip is made to keep wet
Profit, health.
2. the raw material sources that lipstick of the present invention uses are extensive, cheap, make simply, be suitble to mass production, have
Certain market prospects.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1
A kind of anti-inflammatory moist lip protector, including following mass parts substance：It is 62 parts of grease, 22 parts of wax, 6 parts of natural perfume material, molten
3.2 parts of agent, 1 part of moisturizer, 6 parts of natural antiseptic agent, 6 parts of keratolytic, vitamin e1 part, earthworm fibrinolysin 180usp/
100g。
The grease is at least two in mango oil, jojoba seed oil, Sweet Almond Oil, sher butter, tea oil.
The wax is at least two in lanolin wax, cholesterol, cera alba, glyceride, and the acid value of the cera alba is
5.0-8.0。
The natural perfume material is one kind in Rosa Damascana, cherry essence, Lavender, tangerine oil.
The natural antiseptic agent is forsythia suspense extraction, Rosmarinus officinalis extract, thyme extract, extractive of perilla, green tea
At least two in extract.
The moisturizer is at least one of Sodium Hyaluronate, sodium alginate, lactalbumin, casein.
The keratolytic is at least two in evening primrose oil, grape seed oil, cherry nut oil, angle soft-shelled turtle alkane.
The solvent is diisopropyl adipate.
The preparation method of the earthworm fibrinolysin includes the following steps：
A) it chooses wild Pheretimatschiliensis and is rinsed with water surface, be rinsed with water 1h again overnight, dirt is made to be discharged as possible；
B) earthworm after cleaning is inhaled and is twisted into homogenate in high-speed tissue mashing machine, add isometric NH4Ac-HAc
Buffer solution；It is extracted at 4 DEG C overnight, centrifugation takes supernatant；
C) solid (NH4) 2SO4 is added in supernatant to 0.6 saturation degree, saltouts at 4 DEG C for 24 hours, precipitation is collected in centrifugation,
Dialysis is desalted, and 8000r/min centrifuges 30min, and supernatant is crude enzyme liquid；
D) 1 is pressed：Phosphoric acid flushing liquor (PBS) is added in 2 ratio, and homogenate is placed in refrigerator and extracts 6h, centrifuging and taking supernatant；
E) by thick enzyme solutions by DEAE-SepharoseCL-6B ion exchanges, Sephadex G-75 gel chromatographies are received
It is pure enzyme solutions to collect active part.
Embodiment 2
A kind of anti-inflammatory moist lip protector, including following mass parts substance：64 parts of grease, 23 parts of wax, 6.5 parts of natural perfume material,
3.6 parts of solvent, 2 parts of moisturizer, 6.5 parts of natural antiseptic agent, 6.5 parts of keratolytic .2 parts of vitamin e1, earthworm fibrinolysin
185usp/100g。
Embodiment 3
A kind of anti-inflammatory moist lip protector, including following mass parts substance：It is 65 parts of grease, 24 parts of wax, 7 parts of natural perfume material, molten
4 parts of agent, 2.5 parts of moisturizer, 7 parts of natural antiseptic agent, 7 parts of keratolytic .5 parts of vitamin e1, earthworm fibrinolysin 190usp/
100g。
Embodiment 4
A kind of anti-inflammatory moist lip protector, including following mass parts substance：66 parts of grease, 25 parts of wax, 7.5 parts of natural perfume material,
4.4 parts of solvent, 3 parts of moisturizer, 7.5 parts of natural antiseptic agent, 7.5 parts of keratolytic .8 parts of vitamin e1, earthworm fibrinolysin
195usp/100g。
Embodiment 5
A kind of anti-inflammatory moist lip protector, including following mass parts substance：It is 68 parts of grease, 26 parts of wax, 8 parts of natural perfume material, molten
4.8 parts of agent, 4 parts of moisturizer, 8 parts of natural antiseptic agent, 8 parts of keratolytic, vitamin E2 part, earthworm fibrinolysin 200usp/
100g。
Experimental result detects
1. the irritant experiment of intact skin
10 rabbit are taken, are divided into 5 groups two-by-two at random, by its back backbone diamond wool removal, about 150cm2, left side backside
Lipstick 1g/ of embodiment 1-5 is smeared, right side as a contrast, is wrapped up with clean gauze, is washed away with clear water after fixed 4h, daily to apply
Smear primary totally 7 times, 30-60min after last time coating, for 24 hours, 48h, 72h seen whether rubescent, oedema and pigmentation,
Phenomena such as bleeding.
The experimental results showed that 10 rabbit illustrate this product to family without rubescent, oedema and phenomena such as pigmentation, bleeding
Rabbit intact skin is nonirritant.
2. the stimulation test of damaged skin
10 rabbit are taken, are divided into 5 groups two-by-two at random, will be sterilized after its back backbone diamond wool removal, it is broken to draw " well " stroke
Its skin is degree with oozing of blood, smears the lipstick 1g/ of embodiment 1-5 only in left side back unhairing region immediately, right side as pair
According to, it is wrapped up with clean gauze, is washed away with clear water after fixed 4h, it is daily to smear totally 7 times primary, 30- after last time coating
Phenomena such as 60min, for 24 hours, 48h, 72h have seen whether rubescent, oedema and pigmentation, bleeding.
The experimental results showed that 10 rabbit illustrate this product to family without rubescent, oedema and phenomena such as pigmentation, bleeding
Rabbit damaged skin is nonirritant.
3. normal individual trial effect experiment
Application method：Lip is smeared using the lip gloss of the present invention daily, is used continuously one month.
15 people of trier has apparent cheilogramma, the dry and cracked problem of skin of lip that this hair is used continuously there are lip color is obscure
After one month, the dry and cracked situation of skin of lip improves bright lip gloss completely, and skin of lip moistens glossy, and lip color is more apparent
Ground improves, and no longer black purple, skin of lip is glossy, and cheilogramma is obviously thin out.
Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Cannot the limitation to the scope of the claims of the present invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect range.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of anti-inflammatory moist lip protector, which is characterized in that include following mass parts substance：62-68 parts of grease, 22-26 parts of wax,
6-8 parts of natural perfume material, 3.2-4.8 parts of solvent, 1-4 parts of moisturizer, 6-8 parts of natural antiseptic agent, 6-8 parts of keratolytic, dimension life
It is E1-2 parts plain, earthworm fibrinolysin 180-200usp/100g.
2. a kind of anti-inflammatory moist lip protector according to claim 1, which is characterized in that include following mass parts substance：Oil
64-66 parts of fat, 23-25 parts of wax, 6.5-7.5 parts of natural perfume material, 3.6-4.4 parts of solvent, 2-3 parts of moisturizer, natural antiseptic agent
6.5-7.5 parts, 6.5-7.5 parts of keratolytic .2-1.8 parts of vitamin e1, earthworm fibrinolysin 185-195usp/100g.
3. a kind of anti-inflammatory moist lip protector according to claim 1 or 2, which is characterized in that the grease be mango oil, suddenly
At least two in bar seed oil, Sweet Almond Oil, sher butter, tea oil suddenly.
4. a kind of anti-inflammatory moist lip protector according to claim 1 or 2, which is characterized in that the wax is lanolin wax, courage
At least two in sterol, cera alba, glyceride, the acid value of the cera alba is 5.0-8.0.
5. a kind of anti-inflammatory moist lip protector according to claim 1 or 2, which is characterized in that the natural perfume material is rose
One kind in essential oil, cherry essence, Lavender, tangerine oil.
6. a kind of anti-inflammatory moist lip protector according to claim 1 or 2, which is characterized in that the natural antiseptic agent is to connect
Stick up at least two in extract, Rosmarinus officinalis extract, thyme extract, extractive of perilla, green-tea extract.
7. a kind of anti-inflammatory moist lip protector according to claim 1 or 2, which is characterized in that the moisturizer is hyalomitome
At least one of sour sodium, sodium alginate, lactalbumin, casein.
8. a kind of anti-inflammatory moist lip protector according to claim 1 or 2, which is characterized in that the keratolytic is the moon
See at least two in careless oil, grape seed oil, cherry nut oil, angle soft-shelled turtle alkane.
9. a kind of anti-inflammatory moist lip protector according to claim 1 or 2, which is characterized in that the solvent is adipic acid two
Isopropyl ester.
10. a kind of anti-inflammatory moist lip protector according to claim 1 or 2, which is characterized in that the system of the earthworm fibrinolysin
Preparation Method includes the following steps：
A) it chooses wild Pheretimatschiliensis and is rinsed with water surface, be rinsed with water 1h again overnight, dirt is made to be discharged as possible；
B) earthworm after cleaning is inhaled and is twisted into homogenate in high-speed tissue mashing machine, add isometric NH4Ac-HAc is buffered
Liquid；It is extracted at 4 DEG C overnight, centrifugation takes supernatant；
C) solid (NH is added in supernatant4)2SO4It to 0.6 saturation degree, saltouts at 4 DEG C for 24 hours, precipitation is collected in centrifugation, and dialysis is gone
Salt, 8000r/min centrifuge 30min, and supernatant is crude enzyme liquid；
D) 1 is pressed：Phosphoric acid flushing liquor (PBS) is added in 2 ratio, and homogenate is placed in refrigerator and extracts 6h, centrifuging and taking supernatant；
E) by thick enzyme solutions by DEAE-SepharoseCL-6B ion exchanges, Sephadex G-75 gel chromatographies are collected and are lived
Property part is pure enzyme solutions.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810430108.2A CN108553338A (en) | 2018-05-07 | 2018-05-07 | A kind of anti-inflammatory moist lip protector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810430108.2A CN108553338A (en) | 2018-05-07 | 2018-05-07 | A kind of anti-inflammatory moist lip protector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108553338A true CN108553338A (en) | 2018-09-21 |
Family
ID=63538369
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201810430108.2A Withdrawn CN108553338A (en) | 2018-05-07 | 2018-05-07 | A kind of anti-inflammatory moist lip protector |
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| Country | Link |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108969450A (en) * | 2018-10-31 | 2018-12-11 | 长沙浩然医疗科技有限公司 | Edible moisten of one kind is not easy the lipstick and preparation method thereof that decolourizes |
| CN109875915A (en) * | 2019-03-18 | 2019-06-14 | 广州尚庄化妆品有限公司 | Color craftsman's streamer dazzles color lipstick and preparation method thereof |
| CN114191362A (en) * | 2021-12-28 | 2022-03-18 | 福建省梦娇兰日用化学品有限公司 | Children soothing and moisturizing lip balm and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1332243A (en) * | 2001-07-10 | 2002-01-23 | 华南农业大学 | Extraction of lumbricus plasmin |
| CN1593380A (en) * | 2004-06-18 | 2005-03-16 | 中国医科大学 | Face beautifying skin care compositions, its preparation method and uses |
| CN107519125A (en) * | 2017-10-12 | 2017-12-29 | 广州番禺职业技术学院 | One kind has moisturizing effect lipstick and preparation method thereof |
-
2018
- 2018-05-07 CN CN201810430108.2A patent/CN108553338A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1332243A (en) * | 2001-07-10 | 2002-01-23 | 华南农业大学 | Extraction of lumbricus plasmin |
| CN1593380A (en) * | 2004-06-18 | 2005-03-16 | 中国医科大学 | Face beautifying skin care compositions, its preparation method and uses |
| CN107519125A (en) * | 2017-10-12 | 2017-12-29 | 广州番禺职业技术学院 | One kind has moisturizing effect lipstick and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108969450A (en) * | 2018-10-31 | 2018-12-11 | 长沙浩然医疗科技有限公司 | Edible moisten of one kind is not easy the lipstick and preparation method thereof that decolourizes |
| CN109875915A (en) * | 2019-03-18 | 2019-06-14 | 广州尚庄化妆品有限公司 | Color craftsman's streamer dazzles color lipstick and preparation method thereof |
| CN114191362A (en) * | 2021-12-28 | 2022-03-18 | 福建省梦娇兰日用化学品有限公司 | Children soothing and moisturizing lip balm and preparation method thereof |
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Application publication date: 20180921 |
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