CN108507623A - The environment network of silver ion content is detected based on gold nano grain - Google Patents

Abstract

The invention discloses an environment network for detecting silver ion content based on gold nanoparticles, which comprises a master node computer (10), and the master node computer (10) is connected with a plurality of sensors of preset types through wireless signals; the preset A plurality of sensors of the preset type are installed in the thermal pipeline (3) and the water supply pipeline (6) laid in the underground space (100) and on the side wall of the underground space; the plurality of sensors of the preset type are used to collect the corresponding Detection data of underground space. The environmental network for detecting the content of silver ions based on gold nanoparticles disclosed by the present invention can safely and reliably monitor the environment of the underground pipe network in all directions, and especially can effectively monitor the content of silver ions in the water transported by the underground pipe network. Detection can reduce the hidden dangers of underground pipe network, ensure the safe use of underground pipe network, is conducive to wide application, and has great practical significance in production.

Description

An Environmental Network Based on Gold Nanoparticles to Detect Silver Ion Content

technical field

The invention relates to the technical field of underground space environment monitoring, in particular to an environmental network for detecting silver ion content based on gold nanoparticles.

Background technique

At present, the underground pipe network is the top priority in the process of urbanization in my country. It is an important material basis for exerting urban functions and ensuring urban economic and social health and coordinated development. It presents intricate and overlapping three-dimensional characteristics. Pipe network is an important part of urban infrastructure construction, and an important carrier of energy transmission, material transmission, information transmission, drainage and disaster reduction, and waste disposal in a city.

At present, when the environmental water body in the underground pipeline network is polluted, it is easy to significantly increase the content of silver ions in the water body. Silver ion is a highly toxic substance, and its toxic effects mainly include: (1) denaturation of proteins and various enzymes in the human body. (2) Silver ions that enter the body exceed 0.8g and will cause blue silvery spots on the skin. Because silver ions have strong oxidizing properties, silver ions entering the human body can also cause symptoms such as edema of internal organs. In severe cases, it can cause death. The human body does not have an effective silver discharge mechanism. Therefore, once ingested silver ions are mainly accumulated in the bones and liver. And there is no effective antidote for silver ion poisoning. Therefore, the content of silver ions in natural water is the most important in environmental detection and control. In order to protect human health, it is urgent to develop a fast, sensitive and well-selected method to detect silver ions.

For the current underground pipe network, the main problems are: due to the complex spatial structure of the underground pipe network, it is impossible to monitor the environment of the underground pipe network in all directions, especially the content of silver ions in the water transported by the underground pipe network. Effective detection can not reduce the potential safety hazards of the underground pipe network and ensure the safe use of the underground pipe network.

Therefore, there is an urgent need to develop a technology that can safely and reliably monitor the environment of the underground pipe network in all directions, especially the effective detection of the content of silver ions in the water transported by the underground pipe network, which can reduce the underground Potential safety hazards of the pipeline network to ensure the safe use of the underground pipeline network.

Contents of the invention

In view of this, the purpose of the present invention is to provide an environmental network based on gold nanoparticles to detect the content of silver ions, which can safely and reliably monitor the environment of the underground pipe network in all directions, especially the water that can be transported by the underground pipe network. The effective detection of the content of silver ions can reduce the potential safety hazards of the underground pipe network and ensure the safe use of the underground pipe network, which is conducive to wide application and has great practical significance in production.

For this reason, the present invention provides the environmental network that detects silver ion content based on gold nanoparticle, comprises main node computer, is wireless signal connection between the multiple sensors of described main node computer and preset kind;

A plurality of sensors of the preset type are installed in heat pipes and water supply pipes laid in the underground space and on the side walls of the underground space;

The multiple sensors of the preset type are used to collect detection data of the corresponding underground space.

Wherein, the plurality of sensors of described preset kind comprises: first temperature sensor, second flow sensor and surface-enhanced Raman SERS water quality sensor;

The first temperature sensor, the second flow sensor and the surface-enhanced Raman SERS water quality sensor are installed in the water supply pipeline to detect the temperature, flow and silver ion content of the water in the water supply pipeline, and then send them to the master node computer.

Wherein, the multiple sensors of the preset type also include: a first pressure sensor, a first flow sensor and a second temperature sensor;

The first pressure sensor, the first flow sensor and the second temperature sensor are installed in the thermal pipeline to detect the pressure, flow and temperature of the medium transmitted in the thermal pipeline, and then send them to the master node computer.

Wherein, the multiple sensors of the preset types also include: oxygen sensor, carbon monoxide sensor, hydrogen sulfide sensor, temperature and humidity sensor and nitrogen oxide sensor;

The oxygen sensor, carbon monoxide sensor, hydrogen sulfide sensor, temperature and humidity sensor and nitrogen oxide sensor are installed on the side wall of the underground space respectively, and are used to collect the oxygen gas concentration, carbon monoxide gas concentration, hydrogen sulfide gas concentration, Temperature, humidity, and nitrogen oxide concentration are then sent to the master node computer.

Wherein, it also includes: a first monitoring camera and a second monitoring camera;

The first monitoring camera is located at the upper right of the second monitoring camera;

The first monitoring camera and the second monitoring camera are installed on the side wall of the underground space, and are respectively used to collect images of the covered area, and then send them to the main node computer.

Wherein, the master node computer includes: a data storage module, a data processing module and a wireless data transmission module, wherein:

The data storage module is used to store the detection data of the underground space collected by a plurality of sensors of the preset type in real time;

The data processing module is connected with the data storage module, and is used to compare the detection data of the underground space collected by the plurality of sensors of the preset type with the preset and corresponding normal value ranges respectively. If it is within the corresponding normal value range, the corresponding data is judged to be qualified, otherwise, the corresponding data is judged to be unqualified, and at the same time, the comparison situation is sent to the computer of the ground base station through the wireless data transmission module in real time;

The data processing module is also used for sending the images collected by the first monitoring camera and the second monitoring camera to the computer of the ground base station through the wireless data transmission module.

Wherein, also include: inspection robot, described inspection robot is arranged on the ground between thermal pipeline and water supply pipeline.

Wherein, the surface-enhanced Raman SERS water quality sensor includes a test kit and a detection switch, wherein:

A detection switch is installed on the water supply pipeline;

The reagent box is located below the detection switch, and the top of the reagent box has a plurality of holes;

A surface-enhanced Raman SERS chip is inserted into the kit, and the surface-enhanced Raman SERS chip includes a silicon dioxide substrate, and a plurality of DNA and 2-naphthylthiol are arranged at intervals on the silicon dioxide substrate. Modified gold nanoparticles distribution area;

Each gold nanoparticle distribution area modified by DNA and 2-naphthylthiol is correspondingly arranged and communicated with a hole;

An optical fiber probe is arranged directly above the kit, and the optical fiber probe is connected with a portable Raman detector through an optical fiber;

The portable Raman detector is provided with an antenna.

Wherein, the preparation of the surface-enhanced Raman SERS chip comprises the following steps:

The first step, the chloroauric acid solution that is 0.01% by mass concentration is heated and boiled under vigorous stirring, and 1% trisodium citrate aqueous solution is added rapidly under stirring state, the chloroauric acid solution and trisodium citrate aqueous solution The volume ratio is 100:3;

Second step, continue heating and boiling for 15 minutes, make the light yellow solution gradually become stable deep red, then the liquid made is cooled to room temperature to obtain gold colloid;

The third step is to add 2-naphthylthiol with a molar volume concentration of 0.01mM to the gold colloidal solution and mix it gently for 10 minutes to obtain a gold nanoparticle separation liquid labeled with 2-naphthylthiol. Minute speed centrifugation for 10 minutes, remove the supernatant, wherein, the volume ratio of the 2-naphthol and gold colloidal solution is 1:100;

The 4th step, use 1mL concentration again and be the phosphate buffer saline suspending of 10mM, obtain the suspended gold colloidal solution, wherein, the volume ratio of phosphate buffer saline and gold colloidal solution is 1:1;

Step 5: Add the preset DNA solution to the suspended gold colloid solution, react for 12 hours, then add a sodium chloride solution with a molar volume concentration of 0.1M for 30 minutes, and then place it at a temperature of 4 degrees Celsius Continue salinization for 6 hours to obtain a mixture, wherein the volume ratio of 2-naphthylthiol to sodium chloride solution is 1:2;

The sixth step, the mixture was centrifuged at 8000 rpm for 30 minutes, the supernatant was removed, and then suspended in a phosphate buffer with a molar volume concentration of 10 mM to obtain DNA and 2-naphthylthiol-modified Gold nanoparticle solution;

The seventh step is to coat the gold nanoparticles modified with DNA and 2-naphthalenethiol on the silica substrate, and then place it in a drying oven for drying treatment, and finally form a gold nanoparticle modified with DNA and 2-naphthalene on the silica substrate. The distribution area of thiophenol-modified gold nanoparticles was prepared to obtain a surface-enhanced Raman SERS chip.

Wherein, in the fifth step, the specific preparation steps of the preset DNA solution are as follows:

The DNA (CCCCCACCTCCCACCCACC) solution with a molar volume concentration of 100uM is mixed with a tris(2-carboxyethyl)phosphine hydrochloride solution with a molar volume concentration of 10mM, wherein the DNA solution is mixed with tris(2-carboxyethyl)phosphine hydrochloride The volume ratio of the salt solution is 20:3, and the volume ratio of the DNA solution to the 2-naphthylthiol in the third step is 1:1, and it is left standing at room temperature for activation for 1 hour to obtain the preset DNA solution.

It can be seen from the above technical solutions provided by the present invention that compared with the prior art, the present invention provides an environmental network based on gold nanoparticles to detect silver ion content, which can safely and reliably monitor the environment of the underground pipe network in all directions , especially the effective detection of the content of silver ions in the water transported by the underground pipe network, which can reduce the safety hazards of the underground pipe network and ensure the safe use of the underground pipe network. It is conducive to wide application and has great practical significance in production.

Description of drawings

Fig. 1 is the structural representation of the environmental network that detects silver ion content based on gold nanoparticle that the present invention provides;

Fig. 2 is based on the data transmission schematic diagram that the environment network that the present invention detects silver ion content based on gold nanoparticle is formed based on ZigBee wireless communication technology;

Fig. 3 is the synoptic sketch map of surface-enhanced Raman (SERS) water quality sensor and water supply pipeline in the environmental network that detects silver ion content based on gold nanoparticle that the present invention provides;

Fig. 4 is the schematic diagram of the structure of surface-enhanced Raman (SERS) chip in the environment network that detects silver ion content based on gold nanoparticle that the present invention provides;

Figure 5 is a schematic diagram of the connection structure between gold nanoparticles and DNA chains in the environmental network based on gold nanoparticles to detect silver ion content provided by the present invention;

Fig. 6 is the Raman signal schematic diagram obtained when surface-enhanced Raman (SERS) chip detects liquid in the environmental network based on gold nanoparticle detection silver ion content provided by the present invention;

In the figure, 1 is the first monitoring camera; 2 is the second monitoring camera; 3 is the thermal pipeline; 4 is the first pressure sensor; 5 is the first temperature sensor; 6 is the water supply pipeline; 7 is the inspection robot; 8 is the second A flow sensor; 9 is the second flow sensor; 10 is the main node computer; 11 is the second temperature sensor; 12 is the surface-enhanced Raman (SERS) water quality sensor; 13 is the oxygen sensor; 14 is the carbon monoxide sensor; 15 is the hydrogen sulfide Sensor; 16 is a temperature and humidity sensor; 17 is a nitrogen oxide sensor;

18 is a detection switch; 19 is a surface-enhanced Raman (SERS) chip; 20 is a test kit; 21 is a portable Raman detector; 22 is an antenna; 23 is an optical fiber probe; The distribution area of gold nanoparticles; 25 is a silica substrate; 26 is gold nanoparticles; 2 and 7 are DNA chains.

Detailed ways

In order to enable those skilled in the art to understand the solution of the present invention in an alternative way, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

Referring to Fig. 1 to Fig. 6, the present invention provides the environmental network that detects silver ion content based on gold nanoparticle, comprises main node computer 10, between described main node computer 10 and a plurality of sensors of pre-set types (that is, as secondary nodes) The space is connected by wireless signal (specifically through ZigBee wireless communication technology);

A plurality of sensors of the preset type are installed in the thermal pipeline 3 and the water supply pipeline 6 laid in the underground space 100 (i.e. the underground pipe network space) and on the side walls of the underground space;

The plurality of sensors of the preset type are used to collect the detection data of the corresponding underground space, for example, the temperature sensor is used to detect the temperature data of the installed location.

In the present invention, specifically implemented, the multiple sensors of the preset category include: a first temperature sensor 5, a second flow sensor 9 and a surface-enhanced Raman SERS water quality sensor 12;

The first temperature sensor 5, the second flow sensor 9 and the surface-enhanced Raman SERS water quality sensor 12 are installed in the water supply pipeline 6 for detecting the temperature, flow rate and silver ion content of the water in the water supply pipeline 6, Then send it to the master node computer 10 (specifically through the ZigBee wireless communication technology).

In the present invention, specifically implemented, the plurality of sensors of the preset type also includes: a first pressure sensor 4, a first flow sensor 8 and a second temperature sensor 11;

The first pressure sensor 4, the first flow sensor 8 and the second temperature sensor 11 are installed in the thermal pipeline 3 for detecting the pressure of the medium (such as hot water or steam) in the thermal pipeline 3, The flow and temperature are then sent to the master node computer 10 (specifically through ZigBee wireless communication technology, such as ZigBee ad hoc network).

It should be noted that heat pipes are transmission pipes for high-temperature gas or liquid, such as heating pipes in cities.

In the present invention, in specific implementation, the multiple sensors of the preset types also include: oxygen sensor 13, carbon monoxide sensor 14, hydrogen sulfide sensor 15, temperature and humidity sensor 16 and nitrogen oxide sensor 17;

The oxygen sensor 13, the carbon monoxide sensor 14, the hydrogen sulfide sensor 15, the temperature and humidity sensor 16 and the nitrogen oxide sensor 17 are respectively installed on the side walls of the underground space for collecting the overall environmental information of the underground space, specifically for Collect the oxygen gas concentration, carbon monoxide gas concentration, hydrogen sulfide gas concentration, temperature, humidity and nitrogen oxide concentration in the underground space, and then send them to the master node computer 10 (specifically through ZigBee wireless communication technology, such as ZigBee ad hoc network) .

In the present invention, in specific implementation, the underground space environment monitoring network provided by the present invention also includes: a first monitoring camera 1 and a second monitoring camera 2;

The first monitoring camera is located at the upper right of the second monitoring camera 2;

The first monitoring camera 1 and the second monitoring camera 2 are installed on the side wall of the underground space, and are used to collect images of the covered area respectively, and then send them to the master node computer 10 (specifically through ZigBee wireless communication technology, such as ZigBee ad hoc network), so as to realize real-time monitoring of the covered area.

In specific implementation, the first surveillance camera 1 and the second surveillance camera 2 can be surveillance bolts.

It should be noted that, in specific implementation, the first monitoring camera 1 can be located on the ceiling at 15 meters from the entrance of the underground pipe network, and supports 360-degree infrared real-time monitoring. Described second monitoring camera 2 is positioned at 18 meters place side walls of underground pipeline network entrance, can monitor the covered area in real time.

It should be noted that, for the present invention, among the plurality of preset types of sensors, each sensor includes a power supply module, a central control module, an environment collection module and a wireless communication module.

For the present invention, the master node computer 10 includes: a data storage module, a data processing module and a wireless data transmission module, wherein:

The data storage module is used to store the detection data of the underground space collected by a plurality of sensors of the preset type (i.e. as secondary nodes) in real time to form a database;

The data processing module is connected with the data storage module, and is used for detecting the detection data of the underground space collected by a plurality of sensors of the preset type (i.e. as secondary nodes), respectively with the preset and corresponding normal value range ( For example, compare the preset temperature value range and the preset humidity value range), if it is within the preset and corresponding normal value range, it is judged that the corresponding data is qualified; otherwise, it is judged that the corresponding data is unqualified, At the same time, the comparison situation is sent to the computer of the ground base station through the wireless data transmission module in real time, so that the staff on the ground can understand the various environmental parameters of the underground space, obtain the environmental information of the underground space, and realize the real-time monitoring of the underground pipe network . In addition, the data processing module is also used to send the images collected by the first monitoring camera 1 and the second monitoring camera 2 to the computer of the ground base station through the wireless data transmission module.

For the present invention, in order to better monitor the underground space, the underground space environment network provided by the present invention also includes: an inspection robot 7, which is arranged on the ground between the thermal pipeline 3 and the water supply pipeline 6 .

It should be noted that the inspection robot 7 is mainly used for monitoring safety information of underground pipelines and collecting safety information of underground pipelines. The bottom of described inspection robot has wheel, can be equipped with infrared thermal imager, visible light camera and laser navigation module (existing laser navigation module gets final product) on it;

Wherein, by infrared thermal imager, can detect temperature change, thereby can real-time monitor the leakage problem of underground pipelines such as thermal pipeline 3 and water supply pipeline 6;

Visible light cameras are used for real-time monitoring of images in underground spaces, which play an important role in ensuring the safety of pipeline operations.

The laser navigation module is used to ensure the route planning and intelligent detection of the inspection robot 7.

It should be noted that the main structure of the inspection robot 7 for moving and monitoring the environment is the structure of the existing inspection robot, which is similar to the prior art and will not be described here.

On specific implementation, the data storage module, the data processing module and the wireless data transmission module are located at the bottom of the main node computer 10.

It should be noted that, for the present invention, the data of all secondary nodes can be transmitted to the main node computer through the zigbee network, and the data are collected, processed, compared and retrieved through each data module of the main node computer, and finally the obtained Environmental information can be transmitted through the zigbee wireless communication module. Through the data collection, storage, processing and transmission of the whole system, the comprehensive utilization of underground pipe gallery information is realized, which provides strong data support for the overall planning and comprehensive scheduling of underground space.

As shown in Figure 3, for the present invention, described Surface Enhanced Raman (SERS) water quality sensor 12 comprises reagent box 20 and detection switch 18, wherein:

The detection switch 18 is installed on the water supply pipeline 6; specifically, it can be an electromagnetic switch valve;

The test box 20 is located below the detection switch 18, and the top of the test box 20 has a plurality of holes;

Surface-enhanced Raman (SERS) chip 19 is inserted in the reagent box 20, and described surface-enhanced Raman (SERS) chip 19 comprises silicon dioxide substrate 25, and on described silicon dioxide substrate 25, intervals are provided with multiple A gold nanoparticle distribution area 24 modified by DNA and 2-naphthylthiol;

Each gold nanoparticle distribution area 24 modified with DNA and 2-naphthylthiol is correspondingly arranged and communicated with a hole, that is, it can receive the liquid flowing in from the hole;

An optical fiber probe 23 is arranged directly above the reagent box 20, and the optical fiber probe 23 is connected with a portable Raman detector 21 by an optical fiber;

The portable Raman detector 21 is provided with an antenna 22 (specifically, it may be a ZigBee antenna).

In specific implementation, the portable Raman detector 21 can be an existing portable Raman detector. For example, you can refer to the portable Raman detector described in the public specification of the Chinese utility model patent application "A Portable Multifunctional Raman Detector" published on November 9, 2016 with the application number CN201621206981.6.

Therefore, for the present invention, since the detection switch 18 is installed below the water supply pipeline 6, when the detection is started, the detection switch 18 is turned on, and a drop of liquid that flows out from the water supply pipeline 6 will drop on the SERS detection chip 19 in the reagent box 20, Thereby a reaction occurs, and the detection of different pollutants will drop into different holes for detection. The optical fiber probe 23 can emit 532nm laser for Raman detection and processing in the portable Raman detector 21, and finally the data of silver ion content in water detected by the portable Raman detector 21 is wirelessly transmitted to the master node computer through the antenna 22 10.

For the present invention, it should be noted that the surface-enhanced Raman (SERS) water quality sensor 12 is mainly used for the detection of silver ions in water, and the principle of utilization is that silver ions can form "C-Ag2+-C" with C bases in DNA structure, the presence of silver ions was indirectly detected by SERS technology. Since the sulfhydryl group can form a stable Au-S bond with the gold nanoparticles, the 5-end modified sulfhydryl group and C base-rich DNA (DNA sequence is CCCCCACCTCCCACCCACC) can be modified to the surface of the gold nanoparticles.

In order to form a gold nanoparticle distribution area 24 modified with DNA and 2-naphthylthiol on a silicon dioxide substrate 25, and prepare a surface-enhanced Raman (SERS) chip 19, the specific gold nanoparticle modification process includes the following steps :

The first step, the chloroauric acid solution that is 0.01% by mass concentration is heated and boiled under vigorous stirring, and 1% trisodium citrate aqueous solution is added rapidly under stirring state, the chloroauric acid solution and trisodium citrate aqueous solution The volume ratio is 100:3;

Second step, continue to heat and boil 15 minutes, make light yellow solution gradually become stable dark red, then the liquid made is cooled to room temperature, obtains gold colloidal solution. At this point, the gold colloids can be stored in a black bottle at 4°C for later use.

On specific realization, when described chloroauric acid solution is 50mL, corresponding trisodium citrate aqueous solution is 1.5mL, and the diameter of corresponding gold colloid solution can be 30nm.

The third step, adding 2-naphthylthiol (2NT) with a molar volume concentration of 0.01mM (that is, millimoles per liter) to the gold colloidal solution and mixing, stirred gently for 10 minutes to obtain 2-naphthylthiol-labeled gold The nanoparticle separation liquid is then centrifuged at a speed of 8000 rpm for 10 minutes, and the supernatant is removed, wherein the volume ratio of the 2-naphthylthiol (2NT) to the gold colloidal solution is 1:100;

On concrete implementation, when the volume of described 2-naphthylthiol (2NT) is 10uL, corresponding gold colloidal solution needs 1mL.

The fourth step is to suspend with 1 mL of phosphate buffered saline (i.e. PBS, pH 7) with a concentration of 10 mM to obtain a suspended gold colloidal solution, wherein the volume ratio of the phosphate buffered saline and the gold colloidal solution is 1: 1;

Specifically, when the gold colloid solution is 1mL, the selected phosphate buffer is also 1mL.

Step 5, add the preset DNA solution into the suspended gold colloid solution, react for 12 hours, then add a sodium chloride solution with a molar volume concentration of 0.1M (that is, moles per liter) for 30 minutes, and then place Saltification was continued for 6 hours at a temperature of 4 degrees Celsius to obtain a mixture, wherein the volume ratio of 2-naphthylthiol (2NT) to sodium chloride solution was 1:2;

In the fifth step, specifically, when the volume of 2-naphthylthiol (2NT) is 10 uL, the volume of the sodium chloride solution is 20 uL.

In terms of specific implementation, the specific preparation steps of the preset DNA solution are as follows:

The DNA (DNA sequence is CCCCCACCTCCCAC CCACC) solution with a molar volume concentration of 100uM is mixed with a tris(2-carboxyethyl)phosphine hydrochloride (TCEP) solution with a molar volume concentration of 10mM, wherein the DNA solution is mixed with tris(2-carboxy The volume ratio of ethyl) phosphine hydrochloride (TCEP) solution is 20:3, and the volume ratio of DNA solution and 2-naphthylthiol (2NT) in the third step is 1:1, then standing at room temperature for activation 1 hours to obtain a preset DNA solution. For example, when the DNA solution is 10uL, the tris(2-carboxyethyl)phosphine hydrochloride (TCEP) solution is 1.5uL.

The sixth step, the mixture was centrifuged at 8000 rpm for 30 minutes, the supernatant was removed, and then suspended in a phosphate buffer (pH 7) with a molar volume concentration of 10 mM to obtain DNA and 2 -Naphthol-modified gold nanoparticle solution;

The seventh step is to coat the gold nanoparticles modified with DNA and 2-naphthol on the silica substrate, then place them in a drying oven for drying treatment, and finally form the gold nanoparticles modified with DNA and 2-naphthol on the silica substrate 25. - Naphthalenethiol-modified gold nanoparticles distribution area 24 to prepare a surface-enhanced Raman (SERS) chip 19 .

As shown in FIG. 5 , prepared by the above-mentioned method, any one gold nanoparticle 26 is closely connected with the adjacent gold nanoparticles 26 through DNA chains 27.

As shown in FIG. 6 , the abscissa is the Raman shift, which is the wave number difference of the scattered light relative to the incident light, and the ordinate is the photon count, which is the intensity of the scattered light. When there are silver ions in the droplet, the gold nanoparticles aggregate through the "C-Ag ²⁺ -C" structure. After the aggregation, due to the quantum hot spot effect, a strong Raman signal is generated. Its characteristic The peak is mainly located at 1380cm ^-1 , and the silver ion concentration is obtained by the strength of the signal, and the signal is transmitted to the main node computer 10 for processing, so that the rapid generation of drinking mercury ion 1pg/mL (picogram per milliliter) can be realized. In-situ monitoring, with its high precision and in-situ real-time nature, ensures the safety of drinking water for residents, thereby ensuring the safe operation of underground pipe networks.

In summary, compared with the prior art, the environmental network based on gold nanoparticles to detect the content of silver ions provided by the present invention can safely and reliably monitor the environment of the underground pipe network in all directions, especially the Effective detection of the content of silver ions in the water transported by the underground pipe network can reduce the safety hazards of the underground pipe network and ensure the safe use of the underground pipe network, which is conducive to wide application and has great practical significance in production.

The above is only a preferred embodiment of the present invention, it should be pointed out that, for those of ordinary skill in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications can also be made. It should be regarded as the protection scope of the present invention.

Claims (10)

1. detecting the environment network of silver ion content based on gold nano grain, which is characterized in that including host node computer (10), It is connect for wireless signal between the host node computer (10) and multiple sensors of default type；

Multiple sensors of the default type are mounted on the heat distribution pipeline (3) and water supply line being laid in the underground space (100) (6) in and on the side wall of the underground space；

Multiple sensors of the default type are used to acquire the detection data of the corresponding underground space.

2. environment network as described in claim 1, which is characterized in that multiple sensors of the default type include：First Temperature sensor (5), second flow sensor (9) and surface-enhanced Raman SERS water quality sensors (12)；

First temperature sensor (5), second flow sensor (9) and surface-enhanced Raman SERS water quality sensors (12) peace In the water supply line (6), temperature, flow and silver ion content for detecting water in the water supply line (6), so After be sent to host node computer (10).

3. environment network as claimed in claim 2, which is characterized in that multiple sensors of the default type further include：The One pressure sensor (4), first flow sensor (8) and second temperature sensor (11)；

The first pressure sensor (4), first flow sensor (8) and second temperature sensor (11) are mounted on the heating power In pipeline (3), pressure, flow and temperature for detecting institute's transmission medium in the heat distribution pipeline (3) are then sent to main section Point computer (10).

4. environment network as claimed in claim 3, which is characterized in that multiple sensors of the default type further include：Oxygen Gas sensor (13), carbon monoxide transducer (14), hydrogen sulfide sensor (15), Temperature Humidity Sensor (16) and nitrogen oxides pass Sensor (17)；

The oxygen sensor (13), carbon monoxide transducer (14), hydrogen sulfide sensor (15), Temperature Humidity Sensor (16) and NOx sensor (17) is separately mounted on the side wall of the underground space, dense for collecting the oxygen gas in the underground space Degree, carbon monoxide gas concentration, sulfureted hydrogen gas concentration, temperature, humidity and nitrous oxides concentration, are then sent to host node Computer (10).

5. environment network as claimed in claim 4, which is characterized in that further include：First monitoring camera (1) and the second monitoring Camera (2)；

First monitoring camera is located at the upper right side of the second monitoring camera (2)；

First monitoring camera (1) and the second monitoring camera (2) are mounted on the side wall of the underground space, are respectively used to adopt The image for collecting institute overlay area, is then sent to host node computer (10).

6. environment network as claimed in claim 5, which is characterized in that the host node computer (10), including：Data store Module, data processing module and wireless data transfer module, wherein：

Data memory module, the detection data of the underground space for acquiring multiple sensors of the default type, in real time It is stored；

Data processing module is connected with data memory module, the ground for acquiring multiple sensors of the default type The detection data of down space is compared with preset, corresponding normal value range respectively, if positioned at preset, corresponding Normal value range within, then judge corresponding data qualifier, otherwise, then judge corresponding defect of data, meanwhile, in real time Comparable situation is sent to the computer of ground base station by wireless data transfer module；

Data processing module is additionally operable to first monitoring camera (1) and the second monitoring camera (2) acquired image, The computer of ground base station is sent to by wireless data transfer module.

7. such as environment network according to any one of claims 1 to 6, which is characterized in that further include：Crusing robot (7), institute It states on the ground that crusing robot (7) is arranged between heat distribution pipeline (3) and water supply line (6).

8. environment network as claimed in claim 2, which is characterized in that the surface-enhanced Raman SERS water quality sensors (12) Including kit (20) and detection switch (18), wherein：

Detection switch (18) is mounted on the water supply line (6)；

The kit (20) is located at the lower section of detection switch (18), and the top of the kit (20) has multiple holes；

Inserted with surface-enhanced Raman SERS chips (19), the surface-enhanced Raman SERS chips in the kit (20) (19) include silicon dioxide substrates (25), be arranged at intervals on the silicon dioxide substrates (25) multiple by DNA and 2- naphthalene sulphur The gold nano grain distributed areas (24) of phenol modification；

Each gold nano grain distributed areas (24) by the modification of DNA and 2- thionaphthols are correspondingly arranged and are connected with a hole It is logical；

The kit (20) is arranged above a fibre-optical probe (23), and the fibre-optical probe (23) passes through an optical fiber It is connected with a portable Raman detector (21)；

It is provided with antenna (22) on the portable Raman detector (21).

9. environment network as claimed in claim 8, which is characterized in that the preparation of the surface-enhanced Raman SERS chips includes Following steps：

The first step, the chlorauric acid solution ebuillition of heated with vigorous stirring for being 0.01% by mass concentration, it is fast under stirring The volume ratio of the trisodium citrate aqueous solution of speed addition 1%, the chlorauric acid solution and trisodium citrate aqueous solution is 100:3；

Second step continues to heat and boil 15 minutes, and lurid solution is made to gradually become stable peony, then will be manufactured Liquid is cooled to room temperature, and obtains gold colloid；

The 2- thionaphthols of a concentration of 0.01mM of molal volume are added to gold colloid solution mixing third step, are gently mixed 10 points Clock obtains the gold nanoparticle separating liquid of 2- thionaphthols label, then according to 10 points of the speed of 8000 rev/min centrifugation rotation Clock removes supernatant, wherein the volume ratio of the 2- thionaphthols and gold colloid solution is 1:100；

4th step uses the phosphate buffer of a concentration of 10mM of 1mL to suspend again, the gold colloid solution after being suspended, wherein phosphorus The volume ratio of phthalate buffer and gold colloid solution is 1：1；

Preset DNA solution is added in the gold colloid solution after suspending 5th step, reacts 12 hours, then addition mole The sodium chloride solution salinization that volumetric concentration is 0.1M 30 minutes, then be placed in 4 degrees Celsius of temperature and continue salinization 6 hours, it obtains Mixture, wherein the volume ratio of 2- thionaphthols and sodium chloride solution is 1:2；

6th step centrifuges mixture 30 minutes under 8000 rpms of rotating speed, removes supernatant, is subsequently placed in a mole body It suspends in the phosphate buffer of a concentration of 10mM of product, obtains the gold nano grain solution by the modification of DNA and 2- thionaphthols；

7th step will be coated in silicon dioxide substrates by the gold nano grain of DNA and 2- thionaphthols modification, then be placed in drying It is dried in case, finally forms the gold nano grain distributed area by the modification of DNA and 2- thionaphthols in silicon dioxide substrates Domain 24 prepares surface-enhanced Raman SERS chips 19.

10. environment network as claimed in claim 9, which is characterized in that in the 5th step, the tool of the preset DNA solution Preparation step is as follows：

By DNA (CCCCCACCTCCCACCCACC) solution of a concentration of 100uM of molal volume with a concentration of 10mM's of molal volume Three (2- carboxyethyls) phosphonium salt acid salt solutions mix, and the volume ratio of wherein DNA solution and three (2- carboxyethyls) phosphonium salt acid salt solutions is 20:3, and the volume ratio of DNA solution and the 2- thionaphthols in third step is 1:1, room temperature stands activation 1 hour, is preset DNA solution.