CN108444546A - The environment network of silver ion content is detected based on genetic chip and silver nano-grain - Google Patents

Abstract

The invention discloses an environmental network for detecting silver ion content based on a gene chip and silver nanoparticles, comprising a master node computer (10), and the master node computer (10) is connected to a plurality of preset types of sensors by wireless signals; A plurality of sensors of the preset type are installed in the thermal pipeline (3) and the water supply pipeline (6) laid in the underground space (100) and on the side wall of the underground space; the plurality of sensors of the preset type are used for Collect the detection data of the corresponding underground space. The environmental network for detecting silver ion content based on the gene chip and silver nanoparticles disclosed by the present invention can safely and reliably monitor the environment of the underground pipe network in all directions, especially the concentration of silver ions in the water transported by the underground pipe network. The effective detection of the content can reduce the hidden dangers of the underground pipe network, ensure the safe use of the underground pipe network, and is conducive to wide application, which has great practical significance in production.

Description

An Environmental Network Based on Gene Chip and Silver Nanoparticles to Detect Silver Ion Content

technical field

The invention relates to the technical field of underground space environment monitoring, in particular to an environmental network for detecting silver ion content based on gene chips and silver nanoparticles.

Background technique

At present, the underground pipe network is the top priority in the process of urbanization in my country. It is an important material basis for exerting urban functions and ensuring urban economic and social health and coordinated development. It presents intricate and overlapping three-dimensional characteristics. Pipe network is an important part of urban infrastructure construction, and an important carrier of energy transmission, material transmission, information transmission, drainage and disaster reduction, and waste disposal in a city.

At present, when the environmental water body in the underground pipe network is polluted, it is easy to significantly increase the content of silver ions in the water body. Silver ions are highly toxic substances, and their toxic effects mainly include: (1) denaturation of proteins and various enzymes in the human body. (2) More than 0.8g of silver ions entering the body will cause blue silvery spots on the skin. Because silver ions have strong oxidizing properties, silver ions entering the human body can also cause symptoms such as edema of internal organs. In severe cases, it can cause death. The human body does not have an effective mechanism for excreting silver. Therefore, once ingested silver ions are mainly accumulated in the bones and liver. And there is no effective antidote for silver ion poisoning. Therefore, the silver ion content in natural water is the top priority in environmental detection and control. In order to protect human health, it is urgent to develop a fast, sensitive and well-selected method to detect silver ion.

However, a considerable number of cities in my country have not carried out unified layout planning and management of underground pipe networks. The management of underground pipe networks is fragmented, lacks communication with each other, and is in a state of disordered management. Compared with the speed of urban construction. Management is seriously backward and accidents often occur. The consequences of water cuts, power cuts, gas cuts, communication interruptions, fires and explosions caused by accidents not only seriously affect the quality of life and life safety of the people, but also affect the normal progress of economic construction and national defense construction.

For the current underground pipe network, the main problems are: due to the complex spatial structure of the underground pipe network, it is impossible to monitor the environment of the underground pipe network in all directions, especially the content of silver ions in the water transported by the underground pipe network. Effective detection can reduce the potential safety hazards of the underground pipe network and ensure the safe use of the underground pipe network.

Therefore, there is an urgent need to develop a technology that can safely and reliably monitor the environment of the underground pipe network in all directions, especially the effective detection of the content of silver ions in the water transported by the underground pipe network, which can reduce the underground Potential safety hazards of the pipeline network to ensure the safe use of the underground pipeline network.

Contents of the invention

In view of this, the purpose of the present invention is to provide an environmental network based on gene chips and silver nanoparticles to detect silver ion content, which can safely and reliably monitor the environment of the underground pipe network in all directions, especially the ability to monitor the environment of the underground pipe network. The effective detection of the content of silver ions in the transmitted water can reduce the safety hazards of the underground pipe network and ensure the safe use of the underground pipe network, which is conducive to wide application and has great practical significance in production.

For this reason, the present invention provides the environmental network that detects silver ion content based on gene chip and silver nano particle, comprises main node computer, is wireless signal connection between the multiple sensors of described main node computer and preset kind;

A plurality of sensors of the preset type are installed in the heating pipes and water supply pipes laid in the underground space and on the side walls of the underground space;

The multiple sensors of the preset type are used to collect detection data of the corresponding underground space.

Wherein, the multiple sensors of the preset type include: a first temperature sensor, a second flow sensor and a surface-enhanced Raman SERS water quality sensor;

The first temperature sensor, the second flow sensor and the surface-enhanced Raman SERS water quality sensor are installed in the water supply pipeline to detect the temperature, flow and silver ion content of the water in the water supply pipeline, and then send them to the master node computer.

Wherein, the multiple sensors of the preset type also include: a first pressure sensor, a first flow sensor and a second temperature sensor;

The first pressure sensor, the first flow sensor and the second temperature sensor are installed in the thermal pipeline for detecting the pressure, flow and temperature of the medium transported in the thermal pipeline, and then sending them to the master node computer.

Wherein, the multiple sensors of the preset type also include: oxygen sensor, carbon monoxide sensor, hydrogen sulfide sensor, temperature and humidity sensor and nitrogen oxide sensor;

The oxygen sensor, carbon monoxide sensor, hydrogen sulfide sensor, temperature and humidity sensor and nitrogen oxide sensor are installed on the side wall of the underground space respectively, and are used to collect the oxygen gas concentration, carbon monoxide gas concentration, hydrogen sulfide gas concentration, Temperature, humidity, and nitrogen oxide concentration are then sent to the master node computer.

Wherein, it also includes: a first monitoring camera and a second monitoring camera;

The first monitoring camera is located at the upper right of the second monitoring camera;

The first monitoring camera and the second monitoring camera are installed on the side wall of the underground space, and are respectively used to collect images of the covered areas, and then send them to the master node computer.

Wherein, the master node computer includes: a data storage module, a data processing module and a wireless data transmission module, wherein:

The data storage module is used to store the detection data of the underground space collected by the plurality of sensors of the preset type in real time;

The data processing module is connected with the data storage module, and is used to compare the detection data of the underground space collected by the plurality of sensors of the preset type with the preset and corresponding normal value ranges respectively. If it is within the corresponding normal value range, the corresponding data is judged to be qualified, otherwise, the corresponding data is judged to be unqualified, and at the same time, the comparison situation is sent to the computer of the ground base station through the wireless data transmission module in real time;

The data processing module is also used to send the images collected by the first monitoring camera and the second monitoring camera to the computer of the ground base station through the wireless data transmission module.

Wherein, it also includes: an inspection robot, and the inspection robot is arranged on the ground between the thermal pipeline and the water supply pipeline.

Wherein, the surface-enhanced Raman SERS water quality sensor includes a test kit and a detection switch, wherein:

A detection switch is installed on the water supply pipeline;

The reagent box is located below the detection switch, and the top of the reagent box has a plurality of holes;

A surface-enhanced Raman SERS chip is inserted into the kit, and the surface-enhanced Raman SERS chip includes a silicon dioxide substrate, and a plurality of DNA and 2-naphthylthiol are arranged at intervals on the silicon dioxide substrate. Modified silver nanoparticles distribution area;

The surface of the silicon dioxide substrate is sprayed with a silver nano film, and the silver nano film is coated with DNA;

Each silver nanoparticle distribution area modified by DNA and 2-naphthylthiol is correspondingly arranged and communicated with a hole;

An optical fiber probe is arranged directly above the kit, and the optical fiber probe is connected to a portable Raman detector through an optical fiber;

The portable Raman detector is provided with an antenna.

Wherein, preparation is sprayed with silver nano film on the surface and is also coated with the silicon dioxide substrate with DNA on the silver nano film, and concrete preparation process comprises the following steps:

First, since the mercapto group can form a stable Au-S bond with the nano-silver, the nano-silver is sprayed onto the surface of the silicon dioxide substrate by ion sputtering to form a silver nano-film;

Next, the DNA (CCCCCACCTCCCACCCACC) solution of the modified thiol group at the 5 end is applied to the surface of the silicon dioxide substrate with silver nanofilm formed on the surface, and reacted for 4 hours at room temperature;

Next, wash the silica substrate three times with a phosphate buffer solution with a molar volume concentration of 0.1M to remove non-specifically bound DNA strands therein;

Finally, the spots on the DNA were coated with a bovine serum albumin solution with a mass concentration of 2.5% and the active sites on the DNA were blocked, then washed with ultrapure water, and dried in a drying oven to finally obtain a silver nano-film sprayed on the surface and The silicon dioxide substrate with DNA is also coated on the silver nano film.

Wherein, the preparation of the surface-enhanced Raman SERS chip comprises the following steps:

The first step, the silver nitrate solution that the molar volume concentration is 1mM and the trisodium citrate solution that the molar volume concentration is 40nM are mixed and put into the container, and under vigorous stirring, the sodium borohydride solution that the molar volume concentration is 112mM is gradually Add dropwise, and the mixed solution gradually changes from transparent to earthy yellow to obtain a silver colloid solution, wherein the volume ratio between the silver nitrate solution, the trisodium citrate solution and the sodium borohydride solution is 50:4:1;

Second step, aging the silver colloid solution obtained for 24 hours to decompose the sodium borohydride in the silver colloid solution;

Step 3: Add 2-naphthylthiol with a molar volume concentration of 0.01mM to the silver colloidal solution and mix, wherein the volume ratio of 2-naphthylthiol to silver colloidal solution is: 1:100, then gently stir for 10 minutes , obtaining 2-naphthylthiol-labeled silver nanoparticles separation solution;

Step 4: Centrifuge the 2-naphthylthiol-labeled silver nanoparticle separation solution at a speed of 8000 rpm for 10 minutes, remove the supernatant, and then suspend it with a phosphate buffer with a molar volume concentration of 10 mM. Obtain the suspended silver colloid solution, wherein the volume ratio of the phosphate buffer solution and the gold-silver colloid solution is 1:1;

Step 5: Add the preset DNA solution into the suspended silver colloid solution, react for 12 hours, then add a sodium chloride solution with a molar volume concentration of 0.1M for 30 minutes, and then place it at a temperature of 4 degrees Celsius Continue salinization for 6 hours to obtain a mixture, wherein the volume ratio of 2-naphthylthiol to sodium chloride solution is 1:2;

The sixth step, the mixture was centrifuged at 8000 rpm for 30 minutes, the supernatant was removed, and then placed in a phosphate buffer with a molar volume concentration of 10 mM to suspend to obtain DNA and 2-naphthylthiol-modified Silver nanoparticles solution;

The seventh step is to coat the silver nanoparticles modified with DNA and 2-naphthalenethiol on the silica substrate, and then place it in a drying oven for drying treatment, and finally form a silver nanoparticle modified with DNA and 2-naphthalene on the silica substrate. The distribution area of thiophenol-modified silver nanoparticles is prepared to obtain a surface-enhanced Raman SERS chip;

Wherein, in the fifth step, the specific preparation steps of the preset DNA solution are as follows:

A DNA (CCCCCACCTCCCTCCCACCT) solution with a molar volume concentration of 100uM is mixed with a tris(2-carboxyethyl)phosphine hydrochloride solution with a molar volume concentration of 10mM, wherein the DNA solution is mixed with tris(2-carboxyethyl)phosphine hydrochloride The volume ratio of the solution is 20:3, and the volume ratio of the DNA solution to 2-naphthylthiol in the third step is 1:1, and the DNA solution is obtained by standing at room temperature for 1 hour for activation.

It can be seen from the above technical solutions provided by the present invention that compared with the prior art, the present invention provides an environmental network based on gene chips and silver nanoparticles to detect silver ion content, which can safely and reliably monitor the environment of underground pipe networks. Azimuth monitoring, especially the ability to effectively detect the content of silver ions in the water transported by the underground pipe network, can reduce the safety hazards of the underground pipe network and ensure the safe use of the underground pipe network, which is conducive to wide application and has significant production practice significance.

Description of drawings

Fig. 1 is the structural representation of the environmental network that detects silver ion content based on gene chip and silver nanoparticle that the present invention provides;

Fig. 2 is the data transmission schematic diagram that the environmental network based on gene chip and silver nanoparticle detection silver ion content provided by the present invention is formed based on ZigBee wireless communication technology;

Fig. 3 is the synoptic schematic diagram of the cooperative working state of surface-enhanced Raman (SERS) water quality sensor and water supply pipeline in the environmental network that detects silver ion content based on gene chip and silver nanoparticle provided by the present invention;

Fig. 4 is the schematic diagram of the structure of surface-enhanced Raman (SERS) chip in the environment network that detects silver ion content based on gene chip and silver nanoparticles provided by the present invention;

5 is a schematic diagram of the connection structure between silver nanoparticles and DNA chains in the environmental network based on gene chip and silver nanoparticles to detect silver ion content provided by the present invention;

6 is a schematic diagram of the Raman signal obtained when the surface-enhanced Raman (SERS) chip detects the liquid in the environmental network based on the gene chip and silver nanoparticles to detect the silver ion content provided by the present invention;

In the figure, 1 is the first monitoring camera; 2 is the second monitoring camera; 3 is the thermal pipeline; 4 is the first pressure sensor; 5 is the first temperature sensor; 6 is the water supply pipeline; 7 is the inspection robot; 8 is the second A flow sensor; 9 is the second flow sensor; 10 is the main node computer; 11 is the second temperature sensor; 12 is the surface enhanced Raman (SERS) water quality sensor; 13 is the oxygen sensor; 14 is the carbon monoxide sensor; 15 is the hydrogen sulfide Sensor; 16 is a temperature and humidity sensor; 17 is a nitrogen oxide sensor;

18 is a detection switch; 19 is a surface-enhanced Raman (SERS) chip; 20 is a kit; 21 is a portable Raman detector; 22 is an antenna; 23 is an optical fiber probe; The distribution area of silver nanoparticles; 25 is the silicon dioxide substrate; 26 is the silver nanoparticles; 27 is the DNA chain.

Detailed ways

In order to enable those skilled in the art to better understand the solution of the present invention, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

Referring to Fig. 1 to Fig. 6, the present invention provides the environmental network that detects silver ion content based on gene chip and silver nano particle, comprises master node computer 10, and described master node computer 10 and a plurality of sensors of preset kind (that is, as secondary Nodes) are connected by wireless signals (specifically through ZigBee wireless communication technology);

A plurality of sensors of the preset type are installed in the thermal pipeline 3 and the water supply pipeline 6 laid in the underground space 100 (ie, the underground pipe network space) and on the side walls of the underground space;

The multiple sensors of the preset type are used to collect detection data of the corresponding underground space, for example, a temperature sensor is used to detect temperature data of the installed location.

In the present invention, in specific implementation, the multiple sensors of the preset type include: a first temperature sensor 5, a second flow sensor 9 and a surface-enhanced Raman SERS water quality sensor 12;

The first temperature sensor 5, the second flow sensor 9 and the surface-enhanced Raman SERS water quality sensor 12 are installed in the water supply pipeline 6 for detecting the temperature, flow rate and silver ion content of the water in the water supply pipeline 6, Then send to the master node computer 10 (specifically through ZigBee wireless communication technology).

In the present invention, in terms of specific implementation, the multiple sensors of the preset type also include: a first pressure sensor 4, a first flow sensor 8 and a second temperature sensor 11;

The first pressure sensor 4, the first flow sensor 8 and the second temperature sensor 11 are installed in the thermal pipeline 3 for detecting the pressure of the medium (such as hot water or steam) in the thermal pipeline 3, The flow and temperature are then sent to the master node computer 10 (specifically through ZigBee wireless communication technology, such as ZigBee ad hoc network).

It should be noted that a thermal pipeline is a transmission pipeline for high-temperature gas or liquid, such as a heating pipeline in a city.

In the present invention, in specific implementation, the multiple sensors of the preset type also include: an oxygen sensor 13, a carbon monoxide sensor 14, a hydrogen sulfide sensor 15, a temperature and humidity sensor 16 and a nitrogen oxide sensor 17;

The oxygen sensor 13, the carbon monoxide sensor 14, the hydrogen sulfide sensor 15, the temperature and humidity sensor 16 and the nitrogen oxide sensor 17 are respectively installed on the side walls of the underground space for collecting the overall environmental information of the underground space, specifically for Collect the oxygen gas concentration, carbon monoxide gas concentration, hydrogen sulfide gas concentration, temperature, humidity and nitrogen oxide concentration in the underground space, and then send them to the master node computer 10 (specifically through ZigBee wireless communication technology, such as ZigBee ad hoc network) .

In the present invention, in specific implementation, the underground space environment monitoring network provided by the present invention further includes: a first monitoring camera 1 and a second monitoring camera 2;

The first monitoring camera is located at the upper right of the second monitoring camera 2;

The first monitoring camera 1 and the second monitoring camera 2 are installed on the side wall of the underground space, and are respectively used to collect images of the covered area, and then send them to the master node computer 10 (specifically through ZigBee wireless communication technology, such as ZigBee ad hoc network), so as to realize real-time monitoring of the covered area.

In specific implementation, the first surveillance camera 1 and the second surveillance camera 2 may be surveillance bolts.

It should be noted that, in terms of specific implementation, the first monitoring camera 1 can be placed on the ceiling 15 meters from the entrance of the underground pipe network, and supports 360-degree infrared real-time monitoring. The second monitoring camera 2 is located on the side wall at 18 meters from the entrance of the underground pipe network, and can monitor the covered area in real time.

It should be noted that, for the present invention, among the plurality of preset types of sensors, each sensor includes a power supply module, a central control module, an environment collection module and a wireless communication module.

For the present invention, the master node computer 10 includes: a data storage module, a data processing module and a wireless data transmission module, wherein:

The data storage module is used to store the detection data of the underground space collected by the plurality of sensors of the preset type (i.e. as secondary nodes) in real time to form a database;

The data processing module is connected with the data storage module, and is used for detecting the detection data of the underground space collected by a plurality of sensors of the preset type (i.e. as secondary nodes), respectively with the preset and corresponding normal value range ( For example, comparing the preset temperature value range and the preset humidity value range), if it is within the preset and corresponding normal value range, it is judged that the corresponding data is qualified; otherwise, it is judged that the corresponding data is unqualified, At the same time, the comparison situation is sent to the computer of the ground base station through the wireless data transmission module in real time, so that the staff on the ground can understand the various environmental parameters of the underground space, obtain the environmental information of the underground space, and realize the real-time monitoring of the underground pipe network . In addition, the data processing module is also used to send the images collected by the first monitoring camera 1 and the second monitoring camera 2 to the computer of the ground base station through the wireless data transmission module.

For the present invention, in order to better monitor the underground space, the underground space environment network provided by the present invention also includes: an inspection robot 7, which is arranged on the ground between the thermal pipeline 3 and the water supply pipeline 6 .

It should be noted that the inspection robot 7 is mainly used for monitoring safety information of underground pipelines and collecting safety information of underground pipelines. The bottom of described inspection robot has wheel, can be installed with infrared thermal imager, visible light camera and laser navigation module (existing laser navigation module gets final product) on it;

Among them, the infrared thermal imager can detect temperature changes, so that the leakage of underground pipelines such as thermal pipeline 3 and water supply pipeline 6 can be monitored in real time;

Visible light cameras are used for real-time monitoring of images in underground spaces, which play an important role in ensuring the safety of pipeline operations.

The laser navigation module is used to ensure the route planning and intelligent detection of the inspection robot 7.

It should be noted that the main structure of the inspection robot 7 for moving and monitoring the environment is the structure of the existing inspection robot, which is similar to the prior art and will not be described here.

In terms of specific implementation, the data storage module, data processing module and wireless data transmission module are located at the lower part of the master node computer 10 .

It should be noted that, for the present invention, the data of all secondary nodes can be transmitted to the main node computer through the zigbee network, and the data are collected, processed, compared and retrieved through each data module of the main node computer, and finally the obtained Environmental information can be transmitted through the zigbee wireless communication module. Through the data collection, storage, processing and transmission of the whole system, the comprehensive utilization of underground pipe gallery information is realized, and strong data support is provided for the overall planning and comprehensive scheduling of underground space.

As shown in Figure 3, for the present invention, described Surface Enhanced Raman (SERS) water quality sensor 12 comprises reagent box 20 and detection switch 18, wherein:

The detection switch 18 is installed on the water supply pipeline 6; specifically, it can be an electromagnetic switch valve;

The reagent box 20 is located below the detection switch 18, and the top of the reagent box 20 has a plurality of holes;

Surface-enhanced Raman (SERS) chip 19 is inserted in the reagent box 20, and described surface-enhanced Raman (SERS) chip 19 comprises silicon dioxide substrate 25, and on described silicon dioxide substrate 25, intervals are provided with multiple A silver nanoparticle distribution area 24 modified by DNA and 2-naphthylthiol;

The surface of the silicon dioxide substrate 25 is spray-coated with a silver nano-film, and the silver nano-film is coated with (specifically: coated by dots) DNA;

Each silver nanoparticle distribution area 24 modified by DNA and 2-naphthylthiol is correspondingly arranged and communicated with a hole, that is, it can receive the liquid flowing in from the hole;

An optical fiber probe 23 is arranged directly above the reagent box 20, and the optical fiber probe 23 is connected to a portable Raman detector 21 through an optical fiber;

The portable Raman detector 21 is provided with an antenna 22 (specifically, it may be a ZigBee antenna).

In terms of specific implementation, the portable Raman detector 21 may be an existing portable Raman detector. For example, you can refer to the portable Raman detector described in the publication specification of the Chinese Utility Model Patent Application "A Portable Multifunctional Raman Detector" published on November 9, 2016 with the application number CN201621206981.6.

Therefore, for the present invention, since the detection switch 18 is installed below the water supply pipeline 6, when the detection is started, the detection switch 18 is turned on, and a drop of liquid that flows out from the water supply pipeline 6 will drop on the SERS detection chip 19 in the reagent box 20, As a result, a reaction occurs, and different pollutants are detected by dropping into different holes for detection. The optical fiber probe 23 can emit 532nm laser for Raman detection and processing in the portable Raman detector 21, and finally the data of silver ion content in water detected by the portable Raman detector 21 is wirelessly transmitted to the master node computer through the antenna 22 10.

For the present invention, it should be noted that the surface-enhanced Raman (SERS) water quality sensor 12 is mainly used for the detection of silver ions in water, and the principle of utilization is that silver ions can form "C-Ag ²⁺ - C" structure, through the SERS technology to indirectly detect the presence of silver ions.

In order to prepare the silicon dioxide substrate 25 that is spray-coated with silver nano-film on the surface and also coated with (specifically: by point coating) DNA on the silver nano-film, the specific preparation process may include the following steps:

First, since the mercapto group can form a stable Au-S bond with the nano-silver, the nano-silver is sprayed onto the surface of the silicon dioxide substrate by ion sputtering to form a silver nano-film;

Next, the DNA (sequence is CCCCCACCTCCCACCCACC) solution of 5-terminal modified mercapto group is coated on the surface of the silicon dioxide substrate (i.e. the treated substrate surface) with silver nanofilm formed on the surface, and reacted at room temperature for 4 hours;

Next, wash the silica substrate three times with a phosphate buffer solution (PBS) with a molar volume concentration of 0.1M to remove non-specifically bound DNA strands therein;

Finally, use the bovine serum albumin (BSA) solution with mass concentration of 2.5% to coat the spots on the DNA and block the active sites on the DNA, then wash with ultrapure water (also known as UP water), and dry with a drying oven, Finally, a silicon dioxide substrate 25 whose surface is spray-coated with silver nano-film and DNA is coated (specifically: dot-coated) on the silver nano-film is obtained. At this time, you can continue to store it in a 4-degree environment for later use.

In order to form a silver nanoparticle distribution area 24 modified with DNA and 2-naphthylthiol on a silicon dioxide substrate 25, prepare a surface-enhanced Raman (SERS) chip 19, and the specific silver nanoparticle modification process includes the following steps :

The first step, the silver nitrate solution that the molar volume concentration is 1mM and the trisodium citrate solution that the molar volume concentration is 40nM are mixed and put into the container, and under vigorous stirring, the sodium borohydride solution that the molar volume concentration is 112mM is gradually Add dropwise, the mixed solution gradually becomes khaki from transparent, illustrates that silver nanoparticles have been generated, and silver colloidal solution is obtained, wherein, the volume ratio between silver nitrate solution, trisodium citrate solution and sodium borohydride solution is 50:4: 1;

Before the first step, the container to be used can be processed, specifically: take a 100mL Erlenmeyer flask and wash it with water, soak it in aqua regia overnight, then wash it and dry it for later use.

Specifically, when the silver nitrate solution is 20mL, the corresponding trisodium citrate solution is 1.6mL, the corresponding sodium borohydride solution is 0.4mL, and the diameter of the corresponding silver colloid solution obtained can be 30nm.

The second step is to age the obtained silver colloid solution for 24 hours to decompose the sodium borohydride in the silver colloid solution; at this time, it can also be stored at 4 degrees Celsius for future use.

The third step: adding 2-naphthol (2NT) with a molar volume concentration of 0.01mM to the silver colloid solution and mixing, wherein the volume ratio of 2-naphthol (2NT) to the silver colloid solution is: 1:100, Then gently stirred for 10 minutes to obtain a 2-naphthylthiol-labeled silver nanoparticle separation solution;

Specifically, when 2-naphthylthiol (2NT) is 10uL, the corresponding silver colloid solution is 1mL.

The fourth step: the silver nanoparticle separation solution labeled with 2-naphthylthiol was centrifuged at a speed of 8000 rpm for 10 minutes, the supernatant was removed, and then the molar volume concentration was 10 mM phosphate buffer (ie PBS, pH value is 7) suspension, obtains the silver colloid solution after suspension, wherein, the volume ratio of phosphate buffer saline and gold-silver colloid solution is 1:1;

Specifically, when the gold and silver colloidal solution is 1 mL, the selected phosphate buffer is also 1 mL.

Step 5: Add the preset DNA solution into the suspended silver colloid solution, react for 12 hours, then add a sodium chloride solution with a molar volume concentration of 0.1M (that is, moles per liter) for 30 minutes, and then place Saltification was continued for 6 hours at a temperature of 4 degrees Celsius to obtain a mixture, wherein the volume ratio of 2-naphthylthiol (2NT) to sodium chloride solution was 1:2;

In the fifth step, specifically, when the volume of 2-naphthylthiol (2NT) is 10 uL, the volume of the sodium chloride solution is 20 uL.

In terms of specific implementation, the specific preparation steps of the preset DNA solution are as follows:

A solution of DNA (sequence CCCCCACCTCCCTCCCACCT) with a molar volume concentration of 100uM is mixed with a solution of tris(2-carboxyethyl)phosphine hydrochloride (TCEP) with a molar volume concentration of 10mM, wherein the DNA solution is mixed with tris(2-carboxyethyl) ) The volume ratio of the phosphine hydrochloride (TCEP) solution is 20:3, and the volume ratio of the DNA solution and 2-naphthylthiol (2NT) in the third step is 1:1, and then the normal temperature is left to stand for activation for 1 hour, Get a preset DNA solution. For example, when the DNA solution is 10uL, the tris(2-carboxyethyl)phosphine hydrochloride (TCEP) solution is 1.5uL.

The sixth step, the mixture was centrifuged at a speed of 8000 rpm for 30 minutes, the supernatant was removed, and then suspended in phosphate buffer (pH 7) with a molar volume concentration of 10 mM to obtain DNA and 2 - silver nanoparticle solution modified by naphthol;

The seventh step is to coat the silver nanoparticles modified with DNA and 2-naphthol on the silicon dioxide substrate, and then place it in a drying oven for drying treatment, and finally form a silver nanoparticle modified with DNA and 2-naphthol on the silicon dioxide substrate 25. - Naphthalenethiol-modified silver nanoparticles distribution area 24 to prepare a surface-enhanced Raman (SERS) chip 19 .

As shown in FIG. 5 , after being prepared by the above method, any one silver nanoparticle 26 is closely connected with the adjacent silver nanoparticle 26 through DNA chain 27 .

As shown in Figure 6, the abscissa is the Raman shift, which is the wavenumber difference of the scattered light relative to the incident light, and the ordinate is the photon count, which is the intensity of the scattered light. When there are silver ions in the dropped droplets, as shown in Figure 6, when there are silver ions in the droplets, the silver nanoparticles will adsorb the silver nanoparticles to the surface of the SERS chip through the "C-Ag ²⁺ -C" structure, Afterwards, due to the Raman hotspot effect, a strong Raman signal is generated, and its characteristic peak is mainly located at 1380cm ^-1 . The silver ion concentration is obtained through the strength of the signal, and the signal is transmitted to the master node computer 10 for processing. The present invention newly utilizes the adsorption effect of silver ions and C bases, and designs a detection structure so that the detection limit of silver ions reaches 1pg/mL, which is far lower than the silver ion concentration required in the national standard, and because of its excellent The in-situ and real-time performance can ensure the safety of drinking water and promote the development of digital information in underground space networks.

In summary, compared with the prior art, the environmental network based on the gene chip and silver nanoparticles to detect the content of silver ions provided by the present invention can safely and reliably monitor the environment of the underground pipeline network in all directions, especially It can effectively detect the content of silver ions in the water transported by the underground pipe network, reduce the safety hazards of the underground pipe network, ensure the safe use of the underground pipe network, and is conducive to wide application, which has great practical significance in production.

The above is only a preferred embodiment of the present invention, it should be pointed out that, for those of ordinary skill in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications can also be made. It should be regarded as the protection scope of the present invention.

Claims (10)

1. the environmental network that detects silver ion content based on gene chip and silver nanoparticle, it is characterized in that, comprise main node computer (10), be wireless signal between described main node computer (10) and a plurality of sensors of preset kind connect; A plurality of sensors of the preset type are installed in the thermal pipeline (3) and the water supply pipeline (6) laid in the underground space (100) and on the side wall of the underground space; The multiple sensors of the preset type are used to collect detection data of the corresponding underground space. 2. The environmental network as claimed in claim 1, characterized in that, a plurality of sensors of the preset category include: a first temperature sensor (5), a second flow sensor (9) and a surface-enhanced Raman SERS water quality sensor (12); The first temperature sensor (5), the second flow sensor (9) and the surface-enhanced Raman SERS water quality sensor (12) are installed in the water supply pipeline (6) for detecting The temperature, flow and silver ion content of the water are then sent to the master node computer (10). 3. The environmental network according to claim 2, characterized in that, the plurality of sensors of the preset category also includes: a first pressure sensor (4), a first flow sensor (8) and a second temperature sensor (11 ); The first pressure sensor (4), the first flow sensor (8) and the second temperature sensor (11) are installed in the thermal pipeline (3) for detecting the transmission medium in the thermal pipeline (3) The pressure, flow and temperature are then sent to the master node computer (10). 4. The environmental network according to claim 3, characterized in that, the plurality of sensors of the preset categories also include: oxygen sensor (13), carbon monoxide sensor (14), hydrogen sulfide sensor (15), temperature and humidity sensor (16) and nitrogen oxide sensor (17); The oxygen sensor (13), the carbon monoxide sensor (14), the hydrogen sulfide sensor (15), the temperature and humidity sensor (16) and the nitrogen oxide sensor (17) are respectively installed on the side wall of the underground space, for collecting underground space The oxygen gas concentration, carbon monoxide gas concentration, hydrogen sulfide gas concentration, temperature, humidity and nitrogen oxide concentration in the gas are sent to the master node computer (10) then. 5. The environmental network according to claim 4, further comprising: a first monitoring camera (1) and a second monitoring camera (2); The first monitoring camera is located at the upper right of the second monitoring camera (2); The first monitoring camera (1) and the second monitoring camera (2) are installed on the side wall of the underground space, and are respectively used to collect images of the covered areas, and then send them to the master node computer (10). 6. The environment network as claimed in claim 5, characterized in that, the master node computer (10) includes: a data storage module, a data processing module and a wireless data transmission module, wherein: The data storage module is used to store the detection data of the underground space collected by the plurality of sensors of the preset type in real time; The data processing module is connected with the data storage module, and is used to compare the detection data of the underground space collected by the plurality of sensors of the preset type with the preset and corresponding normal value ranges respectively. If it is within the corresponding normal value range, the corresponding data is judged to be qualified, otherwise, the corresponding data is judged to be unqualified, and at the same time, the comparison situation is sent to the computer of the ground base station through the wireless data transmission module in real time; The data processing module is also used to send the images collected by the first monitoring camera (1) and the second monitoring camera (2) to the computer of the ground base station through the wireless data transmission module. 7. The underground space environment network according to any one of claims 1 to 6, further comprising: an inspection robot (7), and the inspection robot (7) is arranged on the thermal pipeline (3) and On the ground between the water supply pipes (6). 8. environmental network as claimed in claim 2, is characterized in that, described surface enhanced Raman SERS water quality sensor (12) comprises reagent box (20) and detection switch (18), wherein: A detection switch (18), installed on the water supply pipeline (6); The reagent box (20) is located below the detection switch (18), and the top of the reagent box (20) has a plurality of holes; A surface-enhanced Raman SERS chip (19) is inserted in the kit (20), and the surface-enhanced Raman SERS chip (19) includes a silicon dioxide substrate (25), and the silicon dioxide substrate (25 ) are provided with a plurality of DNA and 2-naphthylthiol-modified silver nanoparticles distribution regions (24) at intervals; The surface of the silicon dioxide substrate (25) is spray-coated with a silver nanofilm, and the silver nanofilm is coated with DNA; Each silver nanoparticle distribution area (24) modified by DNA and 2-naphthylthiol is correspondingly arranged and communicated with a hole; An optical fiber probe (23) is arranged directly above the reagent box (20), and the optical fiber probe (23) is connected to a portable Raman detector (21) through an optical fiber; The portable Raman detector (21) is provided with an antenna (22). 9. environmental network as claimed in claim 8, it is characterized in that, preparation is sprayed with silver nano film on the surface and on the silver nano film also coated with the silicon dioxide substrate that is attached with DNA, concrete preparation process comprises the following steps: First, since the mercapto group can form a stable Au-S bond with the nano-silver, the nano-silver is sprayed onto the surface of the silicon dioxide substrate by ion sputtering to form a silver nano-film; Next, the DNA (CCCCCACCTCCCACCCACC) solution of the modified thiol group at the 5 end is applied to the surface of the silicon dioxide substrate with silver nanofilm formed on the surface, and reacted for 4 hours at room temperature; Next, wash the silica substrate three times with a phosphate buffer solution with a molar volume concentration of 0.1M to remove non-specifically bound DNA strands therein; Finally, the spots on the DNA were coated with a bovine serum albumin solution with a mass concentration of 2.5% and the active sites on the DNA were blocked, then washed with ultrapure water, and dried in a drying oven to finally obtain a silver nano-film sprayed on the surface and The silicon dioxide substrate with DNA is also coated on the silver nano film. 10. environment network as claimed in claim 8, is characterized in that, the preparation of described surface enhanced Raman SERS chip comprises the following steps: The first step, the silver nitrate solution that the molar volume concentration is 1mM and the trisodium citrate solution that the molar volume concentration is 40nM are mixed and put into the container, and under vigorous stirring, the sodium borohydride solution that the molar volume concentration is 112mM is gradually Add dropwise, and the mixed solution gradually changes from transparent to earthy yellow to obtain a silver colloid solution, wherein the volume ratio between the silver nitrate solution, the trisodium citrate solution and the sodium borohydride solution is 50:4:1; Second step, aging the silver colloid solution obtained for 24 hours to decompose the sodium borohydride in the silver colloid solution; Step 3: Add 2-naphthylthiol with a molar volume concentration of 0.01mM to the silver colloidal solution and mix, wherein the volume ratio of 2-naphthylthiol to silver colloidal solution is: 1:100, then gently stir for 10 minutes , obtaining 2-naphthylthiol-labeled silver nanoparticle separation solution; Step 4: Centrifuge the 2-naphthylthiol-labeled silver nanoparticle separation solution at a speed of 8000 rpm for 10 minutes, remove the supernatant, and then suspend it with a phosphate buffer with a molar volume concentration of 10 mM. Obtain the suspended silver colloid solution, wherein the volume ratio of the phosphate buffer solution and the gold-silver colloid solution is 1:1; Step 5: Add the preset DNA solution into the suspended silver colloid solution, react for 12 hours, then add a sodium chloride solution with a molar volume concentration of 0.1M for 30 minutes, and then place it at a temperature of 4 degrees Celsius Continue salinization for 6 hours to obtain a mixture, wherein the volume ratio of 2-naphthylthiol to sodium chloride solution is 1:2; The sixth step, the mixture was centrifuged at 8000 rpm for 30 minutes, the supernatant was removed, and then placed in a phosphate buffer with a molar volume concentration of 10 mM to suspend to obtain DNA and 2-naphthylthiol-modified Silver nanoparticles solution; The seventh step is to coat the silver nanoparticles modified with DNA and 2-naphthalenethiol on the silica substrate, and then place it in a drying oven for drying treatment, and finally form a silver nanoparticle modified with DNA and 2-naphthalene on the silica substrate. The distribution area of thiophenol-modified silver nanoparticles is prepared to obtain a surface-enhanced Raman SERS chip; Wherein, in the fifth step, the specific preparation steps of the preset DNA solution are as follows: A DNA (CCCCCACCTCCCTCCCACCT) solution with a molar volume concentration of 100uM is mixed with a tris(2-carboxyethyl)phosphine hydrochloride solution with a molar volume concentration of 10mM, wherein the DNA solution is mixed with tris(2-carboxyethyl)phosphine hydrochloride The volume ratio of the solution is 20:3, and the volume ratio of the DNA solution to 2-naphthylthiol in the third step is 1:1, and the DNA solution is obtained by standing at room temperature for 1 hour for activation.