CN108452369B - Preparation method of medical adhesive with high antibacterial performance - Google Patents

Abstract

The invention relates to a preparation method of a medical adhesive with high antibacterial performance, which is characterized by comprising the following steps: step one, adding sodium alginate, lignin, tannic acid and a stabilizer into sterile water according to the proportion of parts, heating to 100 ℃, stirring for dissolving for 150min, and cooling to room temperature to obtain an aqueous solution A; step two, adding poly (alpha-cyano isobutyl acrylate-alpha-cyano octyl acrylate), polyurethane and polyisoprene into the sea buckthorn seed oil according to the parts ratio, heating to 80 ℃, stirring for 75min, and cooling to room temperature to obtain a sea buckthorn seed oil solution B; step three, uniformly mixing the aqueous solution A and the seabuckthorn seed oil solution B, adding the efficient antibacterial component and the formaldehyde inhibitor according to the proportion by parts, and stirring for 4 hours; and step four, filtering and sterilizing the product obtained in the step three, subpackaging, sealing and packaging to obtain the medical adhesive with high antibacterial performance.

Description

Preparation method of medical adhesive with high antibacterial performance

Technical Field

The invention belongs to the technical field of medical materials, and particularly relates to a medical adhesive with high antibacterial performance and a preparation method thereof.

Background

With medical advances, clinical requirements for surgical techniques and the effects of auxiliary materials are becoming higher and higher. The modern medical idea is to reduce the pain of the patient to the maximum extent and recover the patient at the highest speed, the medical adhesive is a branch of medical macromolecules, biological tissues are taken as an adhesive object, and most tissues and organs of a human body can be partially sutured and ligated by using an adhesive technology without anesthesia in the treatment process; the wound is automatically dropped after healing. Compared with the traditional method, the use of the medical adhesive can effectively shorten the operation time and reduce the pain of the patient.

The main research direction of the current medical adhesive is to improve the bonding strength of various adhesives, however, the biomedical materials applied in the current surgical operation have no bacterial immunity function, are easy to adhere and reproduce bacteria, become the infection source of surrounding tissues, bring huge mental and economic pressure to patients, and endow the medical materials with antibacterial activity is the only effective way for solving the problem. The generation of formaldehyde in the storage and use processes of cyanoacrylate medical adhesive has side effects on organism tissues, and the damage to organisms is increased along with the increase of the concentration of the formaldehyde, thereby seriously influencing the effect and the application of the medical adhesive.

Disclosure of Invention

Based on the prior art, the invention aims to provide a medical adhesive which combines a plurality of effective antibacterial components to greatly improve the antibacterial effect, effectively inhibit formaldehyde generation, avoid wound infection and stimulation to biological tissues and has high antibacterial performance, and a preparation method thereof.

The purpose of the invention is realized by the following technical scheme:

the medical adhesive with high antibacterial performance is characterized by comprising the following components in parts by weight: 45-55 parts of poly (alpha-isobutyl cyanoacrylate-alpha-octyl cyanoacrylate), 24-36 parts of efficient antibacterial component, 2-5 parts of polyurethane, 20-25 parts of polyisoprene, 2-4 parts of sodium alginate, 3-6 parts of lignin, 7-10 parts of tannic acid, 1 part of stabilizer, 1 part of formaldehyde inhibitor, 150 parts of sea-buckthorn seed oil and 180 parts of sterile water;

the high-efficiency antibacterial component comprises 5 parts of specific nano antibacterial component, 12-18 parts of natural antibacterial component and 5-10 parts of traditional Chinese medicine antibacterial component;

the specific nano antibacterial component is prepared by mixing silver-loaded nano titanium dioxide, chlorhexidine acetate-montmorillonite and graphene oxide according to the weight part ratio of 2:2: 1;

the natural antibacterial component comprises 2 parts of a butterfly fruit extract, 2 parts of a aronia melanocarpa extract, 3 parts of a sweet wormwood herb extract, 2 parts of a mulberry bark extract, 2 parts of a citrus peel extract and 2 parts of a moso bamboo leaf extract in parts by weight;

the traditional Chinese medicine antibacterial component comprises, by weight, 1 part of semen euphorbiae lantu extract, 2 parts of Yunnan buddleja extract, 2 parts of Japanese ampelopsis extract, 2 parts of liquorice extract and 1 part of gentiana macrophylla extract.

Further, the formaldehyde inhibitor is formed by combining benzoguanamine and hydantoin according to the weight percentage of 1: 4.

Further, the butterfly fruit extract is an ethanol extract of butterfly fruit, and the specific extraction steps are as follows: pulverizing dried semen Oroxyli, sieving with 60 mesh sieve, adding 18 times of 95% ethanol solution, soaking for 80 hr, filtering, and centrifuging at 3000rpm/min for 15 min; collecting supernatant, concentrating by rotary evaporation to concentration of 1.5g/ml, and sterilizing the concentrated solution with microporous membrane to obtain semen Oroxyli extract.

Further, the preparation method of the sweet wormwood herb extract, the mulberry bark extract, the citrus peel extract and the moso bamboo leaf extract specifically comprises the following steps: drying the raw materials, respectively pulverizing, sieving with 100 mesh sieve to obtain powder, respectively, adding 95% ethanol solution, and ultrasonic extracting at 45 deg.C and 200W for 35min to obtain extractive solution; filtering the extractive solution, and concentrating under reduced pressure to obtain slurry; the slurry was mixed at a ratio of 1: dissolving the raw materials in deionized water at a volume ratio of 12, stirring, shaking, and sterilizing with microporous membrane filter to obtain the extracts.

Further, the preparation method of the aronia melanocarpa extract comprises the following steps: washing mud on the surface of the aronia melanocarpa with flowing water, introducing an ozone generator into sterile water to wash the aronia melanocarpa again, crushing pulp into pulp, adding deionized water according to 40% of the volume of the pulp, adding 2.5% of pectinase at the same time, stirring uniformly, and standing for 40min at 30 ℃; adding the supernatant into 95% ethanol solution, ultrasonic extracting at 40 deg.C and 200W ultrasonic power for 15min, and centrifuging at 4000rpm/min for 10 min; after concentrating the supernatant from the centrifugation to a semi-fluid slurry, the slurry was mixed at a ratio of 1: dissolving the extract in deionized water at a volume ratio of 20, stirring, shaking, and sterilizing with microporous membrane to obtain Aronia melanocarpa extract.

Further, the steps of extracting the moleplant seed extract, the yunnan buddleia extract, the ampelopsis japonica extract, the liquorice extract and the gentiana macrophylla extract are as follows: pulverizing dried semen Euphorbiae, Yunnan Buddleja, radix Ampelopsis, Glycyrrhrizae radix and radix Gentianae Marcrophyllae, sieving with 200 mesh sieve, adding 30 times of 90% ethanol solution, soaking for 75 hr, filtering, and centrifuging at 4000rpm/min for 12 min; collecting supernatant, concentrating by rotary evaporation to concentration of 0.8g/ml, and sterilizing the concentrated solution with microporous membrane filter to obtain Euphorbiae Lathyridis semen extract, YUNNANZUIYUCAO extract, radix Ampelopsis extract, Glycyrrhrizae radix extract and radix Gentianae Marcrophyllae extract.

A preparation method of a medical adhesive with high antibacterial performance is characterized by comprising the following steps:

step one, adding sodium alginate, lignin, tannic acid and a stabilizer into sterile water according to the proportion of parts, heating to 100 ℃, stirring for dissolving for 150min, and cooling to room temperature to obtain an aqueous solution A;

step two, adding poly (alpha-cyano isobutyl acrylate-alpha-cyano octyl acrylate), polyurethane and polyisoprene into the sea buckthorn seed oil according to the parts ratio, heating to 80 ℃, stirring for 75min, and cooling to room temperature to obtain a sea buckthorn seed oil solution B;

step three, uniformly mixing the aqueous solution A and the seabuckthorn seed oil solution B, adding the efficient antibacterial component and the formaldehyde inhibitor according to the proportion by parts, and stirring for 4 hours;

and step four, filtering and sterilizing the product obtained in the step three, subpackaging, sealing and packaging to obtain the medical adhesive with high antibacterial performance.

The medical alpha-cyanoacrylate glue has developed fast because of its unique advantages of single component, no solvent, no need of added catalyst, etc. and may be adhered fast at normal temperature. Moreover, the adhesive has the characteristics of wide adhesive range, high strength and instant curing, and more importantly, the medical adhesive has no toxic or side effect on organisms such as skin and the like. The particularity of the chemical structure determines the excellent property of the alpha-cyanoacrylate in adhesion, and the alpha-carbon atom in the molecular structure CNCH2CCOOR of the alpha-cyanoacrylate compound has extremely strong ability of attracting electrons, so that the chemical reaction can be immediately carried out when the alpha-cyanoacrylate compound contacts with a very small amount of anions. The basic substance of the organism, namely protein amino acid chains, are provided with a large number of electronegative groups-NH 2 and-COOH, and when the basic substance of the organism is contacted with alpha-cyanoacrylate, the reaction can be rapidly carried out, and the principle also enables the medical adhesive to be widely applied to clinic. The effect of α -cyanoacrylates on tissue is strongly related to the type and size of the alkyl group. Generally, the longer the ester carbon chain, the slower the polymerization reaction rate, the less irritation to the tissue, and the better the flexibility of the adhesive layer. The invention selects poly (alpha-cyano isobutyl acrylate-alpha-cyano octyl acrylate) as the main raw material in the system, is prepared by adopting an emulsification method, and has good biocompatibility and excellent antibacterial property. The alpha-cyanoacrylate is sterile, and has high bacteriostatic effect on golden yellow staphylococcus, white staphylococcus, tetrabacillus, bacillus subtilis and the like.

Polyurethane adhesives, which are considered to be one of the most promising biomedical materials to solve these problems, have excellent shear strength and impact resistance characteristics, are suitable for various structural adhesion fields, and have excellent flexibility. The polyurethane adhesive has excellent chemical adhesion with many materials containing active hydrogen because it contains isocyanate and carbamate groups with strong polarity and high chemical activity. The hydrogen bonding between the polyurethane and the bonded material can enhance the molecular cohesion, so that the bonding is firmer. In addition, the polyurethane adhesive also has the characteristics of good toughness, adjustability, simple and convenient bonding process, excellent low-temperature resistance, excellent stability and the like. Therefore, the invention selects a proper amount of added polyurethane components to achieve the purpose of improving the toughness and the bonding strength of the final product, and simultaneously, because the polyurethane has good biocompatibility, the polyurethane can be combined and fused with the antibacterial material more easily, thereby enhancing the antibacterial performance of the whole material.

The polyisoprene is the main rubber component of the natural latex, has high purity, is safe to human bodies, has antibacterial performance and is suitable for the fields of medicine, sanitation and the like with high requirements on the safety and comprehensive performance of latex products. The cyanoacrylate and the polyurethane are added together for modification, so that the stability of the polyisoprene rubber product can be greatly improved, the comprehensive performances of the cyanoacrylate such as polymerizability, cohesiveness, flexibility, various mechanical properties, biocompatibility and the like can be effectively improved, and the final medical rubber product can better meet various requirements of clinical medical rubber.

Titanium dioxide photocatalytic antibacterial agents and silver antibacterial agents are inorganic antibacterial agents, have the characteristics of safety, durability, broad spectrum and the like, and are widely applied in recent years. Nanometer titanium dioxide is also called nanometer titanium dioxide. In terms of size, fine particles generally having a significantly changed physicochemical property are produced, and have a size of 100 nm or less and a white loose powder in appearance. Has the effects of resisting ultraviolet rays, resisting bacteria, self-cleaning and resisting aging, and can be used in the fields of cosmetics, functional fibers, plastics, printing ink, coatings, paints, fine ceramics and the like. Under the action of ultraviolet rays, the malignant hela cells can be thoroughly killed by the ultrafine TiO2 with the concentration of 0.1mg/cm3, and the efficiency of killing cancer cells by TiO2 through photocatalysis is improved along with the increase of the addition amount of superoxide dismutase (SOD); the TiO2 is used for photocatalytic oxidation deep treatment of tap water, so that the number of bacteria in the water can be greatly reduced, and the tap water has no mutagenic effect after drinking, thereby reaching the standard of safe drinking water. The nano TiO2 added into the coating can be used for preparing antibacterial and antifouling coating with the functions of sterilization, antifouling, deodorization and self-cleaning, can be applied to places with dense and easily-propagated bacteria such as hospital wards, operating rooms, family toilets and the like, and can effectively kill harmful bacteria such as escherichia coli, yellow glucose bacteria and the like and prevent infection. Therefore, the nano TiO2 can purify air and has a deodorization function. The nano silver can kill more than 600 germs and can achieve the effect of more than 99 percent. The silver-loaded nano titanium dioxide solution greatly improves the defect of nano titanium dioxide in sterilization. The silver-loaded nano titanium dioxide is an excellent antibacterial material. The silver-loaded nano titanium dioxide antibacterial agent not only has antibacterial property, but also can be used as an anti-ultraviolet and anti-aging agent. The antibacterial agent is added to obtain good antibacterial performance and has long-term antibacterial effect.

Chlorhexidine acetate, also known as chlorhexidine acetate, is a biguanide disinfectant, and consists of chlorhexidine with highly symmetrical straight chain structure combined with two acetic acid molecules. Chlorhexidine acetate is a cationic surface active preservative and has the characteristics of wide antibacterial spectrum and strong antibacterial effect. Chlorhexidine acetate has obvious affinity to bacteria, and the action mechanism of the chlorhexidine acetate is that the chlorhexidine acetate is combined on the surface of the cell membrane of the bacteria, changes the permeability of the membrane, causes the outflow of intracellular substances, the precipitation and the agglutination of cytoplasm, and inhibits the growth of cells so as to kill the cells. A large number of studies at home and abroad show that the chlorhexidine acetate has strong inhibition effect on a plurality of gram-positive and gram-negative bacteria such as escherichia coli, staphylococcus aureus, streptococcus mutans, actinomycetes, pseudomonas aeruginosa and the like, and fungi such as candida albicans. Montmorillonite, also known as bentonite, is a natural mineral with a 2:1 layered silicate crystal structure, each unit cell has a layer of alumino-octahedra sandwiched between two silicon-oxygen tetrahedra connected by common oxygen atoms, and the close packing structure of the tetrahedra and octahedra makes it have highly ordered crystal arrangement with interlayer spacing of about 1 nm. The interlayer is exchangeable inorganic cation such as hydrated Na +, Ca2+, etc. Si4+ in a montmorillonite tetrahedron is often replaced by Al3+, Al3+ in an octahedron is often replaced by Mg2+, Fe3+, Fe2+, Ni2+, Li + and the like, so weak negative charges are generated between layers, and excessive negative charges are balanced by cations such as Na +, Ca2+ and Mg2+ which are dissociated between the layers, so that the montmorillonite is easy to generate organic montmorillonite by exchange reaction with alkyl quaternary ammonium salt or other organic cations. By utilizing the property, the montmorillonite can be combined with antibacterial drugs, biological macromolecules and the like to form a functional composite material, and the nano composite material with the function of controlling the slow release of the drugs is prepared.

The chlorhexidine acetate-montmorillonite nano antibacterial compound is a novel nano antibacterial drug, can effectively enhance the antibacterial property of chlorhexidine acetate and ensure the slow release property of an antibacterial material, and greatly improves the inhibition effect on gram-positive and gram-negative bacteria such as escherichia coli, staphylococcus aureus, streptococcus mutans, pseudomonas aeruginosa and the like and fungi such as candida albicans. Meanwhile, the chlorhexidine acetate-montmorillonite and polyurethane are added together, so that the prepared antibacterial material has long-acting, stable, safe and reliable performance and good mechanical property, and the material has low cost and is easier to process.

Graphene draws extensive attention from researchers in the fields of electronic devices, biology, medicine and the like due to its good electrical conductivity, thermal conductivity, light transmittance, biocompatibility, mechanical strength and the like. Graphene oxide (graphene oxide) is an important derivative of graphene, and the main structure of the graphene oxide is basically the same as that of graphene, except that epoxy bonds and hydroxyl groups are connected to the upper side and the lower side of a two-dimensional space infinitely extending basal plane in monoatomic arrangement, functional groups such as carboxyl groups are connected to the edge of the basal plane, the relationship between the graphene oxide and graphene is similar to that of a functionalized carbon nanotube and a carbon nanotube, and the graphene oxide has various characteristics and more commonality. Due to the high specific surface area and the abundant functional groups on the surface, the graphene oxide has wide application in the aspects of affinity to cells, enhancement to polymer nano materials and antibacterial performance. At present, scholars at home and abroad mainly apply the graphene oxide to the fields of electronic devices, reinforced materials, biomedicine and the like.

The medical material prepared by taking alginate as a raw material has good affinity with wounds, has the characteristic of rapid hemostasis, can be used for repairing tissues and organs of a human body, and has antibacterial property. The chemical molecular formula of sodium alginate is (C6H706Na) n, and the sodium alginate is a natural polysaccharide carbohydrate, and has the physicochemical properties that: the product is tasteless white or light yellow amorphous powder, is easily soluble in water, has strong hygroscopicity, is insoluble in organic solvents such as alcohol and chloroform, and has the characteristics of bioadhesion, biocompatibility, biodegradability and the like. Sodium alginate is used as a base material of drugs in the fields of medicine, biotechnology, and the like. Sodium alginate has been received in the United states pharmacopoeia as early as 1938. Alginic acid was incorporated in the british pharmacopoeia in 1963. Alginic acid is insoluble in water but swells when placed in water. Therefore, sodium alginate is used as a binding agent of tablets in the prior art, has unique advantages in thickening, and can prevent the viscosity reduction phenomenon in the product disinfection process due to good fluidity of sodium alginate. Meanwhile, sodium alginate can also be used as a stabilizer in the system, so that the storage and transportation of the product are more convenient.

The semen Oroxyli is also called as denseflower wall, fructus Pittospori Glabrati, semen Castaneae, and Acer Ginnala; pleasantly shining, pleasantly warm and humid climate, cold resistance, leaf gathering and growing of the top of the small branch, ellipse or oblong ellipse, whole margin, conicity, terminal growth, unisexual and homosexual flower, small male flower, fruit stone-shaped, single-spherical or double-spherical. Phalaenopsis is an arbor, the fruit is in a skewed egg-shaped sphere or double-sphere shape, the flower column base is in a beak shape, the seeds are nearly spherical, and the period of 5-11 months of flowers and fruits is provided. Distributed in the south of Guizhou, the northwest of Guangxi, the west and the southwest, and the southeast of Yunnan in China. It is also in evergreen forest of mountain slope or valley with elevation of 150 + 1000 m mountain or limestone mountain, Vietnam and Burma. The butterfly fruit is a few species, and only one species is used in China, so that the butterfly fruit is an economic tree with both grain and oil. The oil content of the seeds is 33-39%, the protein is 15-18%, the starch is 21-40%, and the sugar content is 2.5-12%. The butterfly fruit extract contains kaempferol, luteolin and other components, has high-efficiency inhibition effect on staphylococcus, escherichia coli, bacillus subtilis and other bacteria, and has strong antibacterial activity. Meanwhile, the methyl o-hydroxybenzoate in the butterfly fruit extract has a local stimulation effect, can promote local blood circulation, and has the effects of diminishing swelling, diminishing inflammation and relieving pain, and the tetrahydroisoquinoline compound has antifungal activity.

Aronia melanocarpa is also called aronia melanocarpa, Rosaceae, Aronia, perennial deciduous shrub. Originally produced in the northeast of north america, the boro coast to the pacific coast are distributed. The fruit contains polyphenol compounds such as procyanidine, anthocyanin and quercetin, and bioactive components such as organic acids, triterpenes and sterol compounds, wherein the total amount of the polyphenol compounds reaches 2.5-3.5%, and is higher than berries such as blueberry, cowberry and cranberry. The Aronia melanocarpa extract has high antibacterial activity on Escherichia coli and Staphylococcus aureus, has antioxidant and anti-inflammatory effects, and can also be used as antiseptic.

Herba Artemisiae Annuae is aerial part of Artemisia annua L of Compositae, and is annual herbaceous plant. The main source of antimalarial artemisinin. The sweet wormwood herb comprises the following chemical components: sesquiterpene lactones: artemisinin compounds such as artemisinin, artesunic acid A, artesunic acid B, and artesunic lactone. And (3) flavonoids: kaempferol (kaempferol), quercetin. The effective components in herba Artemisiae Annuae have high hemostatic effect and remarkable antibacterial effect.

The citrus peel contains a large amount of flavonoids, has the effects of resisting bacteria, diminishing inflammation, resisting allergy, easing pain, resisting viruses, resisting oxidation and resisting cancers, is an excellent raw material used as a medicament, and has excellent antibacterial activity on common bacteria such as escherichia coli, staphylococcus aureus, bacillus subtilis and the like. The citrus peel is produced from orange peel, sour pomelo peel or orange peel in western Hunan province.

The mulberry is a woody plant which grows for many years, the mulberry has been used as a main food source of the silkworm for a long time and plays a vital role in the development of the silk industry, and related researches on the mulberry are also developed around promoting the development of the silk industry. However, besides being a food source for silkworms, mulberry has many other important applications, and mulberry roots, mulberry barks, mulberry leaves and the like can be used as medicines. The mulberry bark contains various rare flavonoid components: the mulberry bark extract contains the components of morin, phellinus igniarius chromene, cyclosporine, sanggenon A-P, mulberrofuran A, B, C, K, N, 0 and M, P, Q, betulinic acid, mulberry polysaccharide and coumarin, and has strong inhibiting effect on staphylococcus aureus; has slight inhibition effect on typhoid bacillus and shigella flexneri. The mulberry bark furan A has stronger bacteriostatic activity on staphylococcus aureus and mycobacterium. Cyclosporin also has antifungal effect.

The antibacterial components of the Phyllostachys Pubescens leaf extract are flavonoids and anthraquinones. The bacteriostatic effect is achieved mainly by acting on the cell membrane of the microorganism. Isoflavone in the phyllostachys pubescens leaf extract can obviously inhibit the catalytic activity of tyrosinase, interrupt the oxidation process of melanin and inhibit the generation and formation of melanin, thereby preventing the formation of scars; the isoflavone has effects in resisting oxidation and enhancing immunity. The Phyllostachys Pubescens leaf contains abundant flavonoids, bioactive polysaccharides and other effective components, such as phenolic acid compounds, anthraquinone compounds, terpene lactone, special amino acids and active peptides, manganese, zinc, selenium, etc. The functional factors contained in the Phyllostachys Pubescens leaf mainly include flavone glycoside and coumarin lactone. The moso bamboo leaf extract has a plurality of excellent biological activity effects of bacteriostasis, free radical resistance, oxidation resistance and the like. The action mechanism of the phyllostachys pubescens leaf extract preservative is actually mainly influenced by the enzyme activity or the genetic particle structure of the cellular protoplasm part. The moso bamboo leaf extract is a natural bacteriostatic preservative, can effectively play a role in preserving as one active component, and does not need to be additionally added with chemical preservative components harmful to the skin.

Qianjin Zi, name of traditional Chinese medicine. Is dried mature seed of Euphorbiaceae plant Euphorbia lathyris L. The semen Euphorbiae contains diterpene alcohol and ester compounds, sterol compounds, coumarin compounds, flavonoid compounds, volatile oil compounds, fatty oil compounds, and other compounds. Has antibacterial, antiinflammatory, analgesic, repercussive, astringent, and hemostatic effects.

The yunnan buddleia is a plant of buddleia of Scrophulariaceae, and is a special plant in China. Is distributed in Yunnan and other places, grows in the area with the altitude of 1,000-2,500 meters, and mostly grows in mountain forest borders and hillside shrubs. The buddleja plant contains more bioactive components, including flavonoids, phenylpropanoid glycosides, terpenoids, saponins, lignans, sterols, long-chain fatty acid esters, alkaloids, etc. The Yunnan buddleja has antibacterial, antiinflammatory, tranquilizing and analgesic effects.

Ampelopsis japonica, also known as Japanese pachyrhizus, Ipomoea batatas, Ampelopsis japonica seedling, white root, Ficus simplicissima lour, etc., is the dry root tuber of Ampelopsis japonica of Vitaceae. Distributed in North China, northeast China, east China, south China, Shaanxi, Ningxia, Sichuan and the like. Collected in spring and autumn, removed silt and fine root, cut into longitudinal petals or oblique pieces, and dried in the sun. The radix Ampelopsis extract has effects of clearing heat and detoxicating, detumescence and resolving hard mass, promoting granulation and relieving pain, anti-inflammatory and antibacterial.

Radix Glycyrrhizae is also called "Gulao", radix Glycyrrhizae, Uralen radix Glycyrrhizae, and radix Glycyrrhizae. Leguminous and glycyrrhiza herbs belong to perennial herbs, and the roots and the rhizomes are strong, so the Chinese herbal medicine is a tonifying Chinese herbal medicine. The medicinal part is root and rhizome, and the medicinal root is cylindrical, 25-100 cm long and 0.6-3.5 cm in diameter. The skin has different elasticity, and the surface is reddish brown or grayish brown. The root and stem are cylindrical, the surface has bud mark, and the middle of the section has marrow. Light smell, sweet and special taste. The functions are mainly used for clearing away heat and toxic material, eliminating phlegm and relieving cough, and treating abdominal cavity and the like. It is a dry climate with preference for darkness and dampness, long sunshine and low temperature. Licorice root is mainly grown in arid and semi-arid desert grassland, desert edge and loess hilly land, and its root and rhizome are used as medicine. Researchers find that the effective components in the liquorice are flavonoids such as liquiritin, liquiritigenin and the like, triterpenoids such as glycyrrhetinic acid, glycyrrhizin and the like and some polysaccharides. The licorice extract has the effects of resisting ultraviolet rays, inhibiting tyrosinase activity, effectively resisting bacteria, improving inflammation and the like, and effectively promoting skin wound healing and improving scar conditions.

Gentiana macrophylla is a perennial herb of Gentianaceae, and is one of the main drugs for treating rheumatic arthralgia and other diseases. The quality of large-leaved gentian produced in Huang nan, especially in Qinghai, is the best. Is one of the important traditional Chinese medicines in China, and was recorded in Shen nong Ben Cao Jing. The gentiana macrophylla has the effects of dispelling wind, clearing away heat and toxic materials, resisting inflammation, inhibiting bacteria, relieving pain and the like. The main components of the extract are iridoid glycosides, triterpenes, sterols and saccharides, and the compounds have good antibacterial property and can effectively promote wound healing and skin growth.

Tannic acid belongs to a typical glucose acyl compound, and the structure of polyphenol hydroxyl group of tannic acid endows the tannic acid with a series of unique chemical characteristics and physiological activities, such as combination with protein, alkaloid and polysaccharide, so that the physicochemical behavior of the tannic acid is changed; can generate complexation and electrostatic interaction with various metal ions; has reducing and free radical capturing activity; has an amphiphilic structure and a plurality of derivatization reaction activities, and the like. The structure of a plurality of ortho phenolic hydroxyl groups of the tannic acid can be used as a polybase ligand to carry out complex reaction with metal ions. Two adjacent phenolic hydroxyl groups can form a stable five-membered ring chelate with metal ions in the form of oxygen anions, and the third phenolic hydroxyl group in the pyrogallol structure can promote the dissociation of the other two phenolic hydroxyl groups although not participating in the complexation, thereby promoting the formation and the stability of the complex. The ortho phenolic hydroxyl group in the pyrogallol structure of tannic acid is easily oxidized into quinone structure, and the oxidation reaction proceeds faster in the presence of enzymes, sufficient moisture, and at higher pH values (e.g., pH > 3.5), thereby consuming oxygen from the environment. The phenolic structure is an excellent hydrogen donor, has obvious inhibition effect on free radicals such as oxygen anions, hydroxyl free radicals and the like, and plays a role in protecting biological tissues. Tannic acid has a good radical trapping function due to the phenolic hydroxyl group, and can be used as an antioxidant. For example, food-grade tannic acid is used as wine antioxidant instead of sulfur dioxide.

The tannic acid contains a unique polyphenol hydroxyl structure, can be combined with biological macromolecules such as protein polysaccharide and the like and can also generate complexation with metal ions, the polyphenol hydroxyl structure contains a catechol structure, so the tannic acid has good reducibility, astringency and antibacterial property, is clinically used for treating local bleeding, and can be used for synthesizing gallic acid and pyrogallol (pyrogallic acid and pyrogallol). Tannic acid is a main raw material for preparing an antibacterial synergist in the pharmaceutical industry.

Lignin is the second biomass resource of the plant kingdom after cellulose, containing about 15-30% lignin in wood and about 12-20% lignin in annual plants; lignin is the only biomass resource in nature which can provide aromatic groups, but lignin can be biodegraded. The lignin has various functional groups and can be directly used as a curing agent, and the lignin is added into the resin to prepare the adhesive, so that the adhesive has good aging resistance and excellent antibacterial performance.

The sea buckthorn seed oil is a brown yellow to brown red transparent oily liquid obtained by subjecting sea buckthorn seeds to supercritical extraction or subcritical low-temperature extraction, and is a high-concentration product integrating active ingredients of sea buckthorn. Contains more than 140 bioactive components such as flavone, organic acid, alkaloid, sterol, triterpene, and various vitamins. The sea-buckthorn seed oil has obvious biological effect, high infection resistance, fast healing speed, and excellent and stable curative effect on tonsillitis, stomatitis, dermatitis, women's cervicitis, etc. The oleum Hippophae is a complex of vitamins and bioactive substances. It has effects of nourishing skin, promoting metabolism, resisting allergy, resisting bacteria, diminishing inflammation, promoting epithelial cell regeneration, repairing skin, maintaining skin acidic environment, and has strong permeability, so it is also important raw material for skin care. The total flavonoids of fructus Hippophae can directly capture superoxide radical and hydroxyl radical, and Ve and Vc superoxide dismutase (SOD) has effects of resisting oxidation, eliminating free radical on cell membrane, and effectively delaying aging. The VC content of sea-buckthorn is at the head of all fruits and vegetables and is known as the king of VC. VC is a natural whitening agent in vivo, can effectively inhibit the deposition of abnormal pigments on the skin and the activity of tyrosinase, and is helpful for the reduction of dopachrome, thereby reducing the formation of melanin and effectively whitening the skin. The sea buckthorn is rich in VE, carotene, carotenoid, beta-sitosterol, unsaturated fatty acid and the like, can inhibit subcutaneous tissue inflammation, enhance the anti-inflammatory effect of an inflammation center and obviously promote ulcer healing.

One class of organic compounds characterized by having a sulfonyl group and usually linked to two carbon atoms by sulfur (e.g., to two hydrocarbyl groups or a simple divalent group) are generally crystallographically stable compounds. Sulfones are often produced by oxidation of sulfides, with the intermediate product being a sulfoxide. The stabilizing agent selected by the invention adopts a composition of sulfone compounds, and the sulfone compounds are very effective antibacterial agents for a plurality of gram-positive and gram-negative bacteria. But the application of various sulfone compounds in various fields is influenced due to the toxicity problem of the various sulfone compounds. The selected sulfone compound composition is a nontoxic substance with excellent performance, and simultaneously, various mechanical properties of the product can be effectively improved by adding a cyanoacrylate substance and polyurethane. 3,3 '-diamino-4, 4' -dihydroxy diphenyl sulfone is nontoxic, can greatly enhance the heat resistance of the product and has good thermal stability; wherein dimethyl sulfolane has high boiling point, good thermal stability and no corrosiveness, and can improve the mechanical properties such as viscosity, flexibility and the like of the product while improving the stability after being added into a system.

Advantageous effects

Due to the adoption of the technical scheme, the invention has the following beneficial effects:

1. the high-antibacterial-performance medical adhesive is prepared by selecting special antibacterial components through repeated experiments, wherein the special antibacterial components comprise three antibacterial systems of 5 parts of special-effect nano antibacterial components, 12-18 parts of natural antibacterial components and 5-10 parts of traditional Chinese medicine antibacterial components, so that the long-acting and high-efficient antibacterial performance of the whole product is comprehensively improved, the comprehensive performance of the medical adhesive is improved, and the risk of a user is greatly reduced.

The silver-loaded nano titanium dioxide selected in the invention fully combines the advantages of two inorganic antibacterial agents and the special performance of a nano material, and simultaneously overcomes the defects that the electron pair of the titanium dioxide of the existing inorganic antibacterial material is easy to be compounded with a hole and the silver of the silver-loaded material is fast released and is easy to discolor; the chlorhexidine acetate-montmorillonite nano antibacterial compound is a novel nano antibacterial drug, and can effectively enhance the antibacterial property of chlorhexidine acetate and ensure the slow release property of an antibacterial material; graphene oxide has great advantages in affinity to cells and antibacterial performance due to its high specific surface area and abundant functional groups on the surface. The three specific antibacterial materials cooperate with each other to effectively ensure the broad-spectrum antibacterial performance, safety and long-term antibacterial effect of the product.

In addition, the montmorillonite and the graphene oxide can effectively improve the mechanical properties of the poly (isobutyl alpha-cyanoacrylate-octyl alpha-cyanoacrylate) and the polyurethane material, and play an effective role in enhancing. Meanwhile, the chlorhexidine acetate-montmorillonite and polyurethane are added together, so that the prepared antibacterial material has long-acting, stable, safe and reliable performance and good mechanical property, and the material has low cost and is easier to process.

2. The natural antibacterial components selected by the medical adhesive with high antibacterial performance comprise 2 parts of butterfly fruit extract, 2 parts of aronia melanocarpa extract, 3 parts of sweet wormwood herb extract, 2 parts of mulberry bark extract, 2 parts of citrus peel extract and 2 parts of lophatherum gracile extract, and the butterfly fruit extract contains kaempferol, luteolin and other components, has high-efficiency inhibiting effect on staphylococcus, escherichia coli, bacillus subtilis and other bacteria, and has strong antibacterial activity. Meanwhile, the methyl o-hydroxybenzoate in the butterfly fruit extract has a local stimulation effect, can promote local blood circulation, and has the functions of detumescence, antiphlogosis and analgesia, and the tetrahydroisoquinoline compounds have antifungal activity. The extract of Aronia melanocarpa contains polyphenols such as procyanidin, anthocyanin, and quercetin, and has high antibacterial activity. The herba Artemisiae Annuae extract has high content of sesquiterpene lactone and flavonoid, and has high hemostatic effect and remarkable antibacterial property. The citrus peel contains a large amount of flavonoids, and has high antibacterial and anti-inflammatory effects. The mulberry bark extract contains natural high-efficiency antibacterial substances such as mulberrin, mulberrypsin, cyclosporine, sanggenon, and mulberrofuran. The flavone glycoside and coumarin lactone in the phyllostachys pubescens leaf extract have excellent biological activity effects of bacteriostasis, free radical resistance, oxidation resistance and the like. The invention fully combines the effective components of six natural plants and fruits, has long-acting and high-efficiency antibacterial effects, has better compatibility to human bodies, and is safe without toxic and side effects.

Meanwhile, the aronia melanocarpa extract and the phyllostachys pubescens extract are natural antibacterial preservatives, can effectively play a role in corrosion prevention as active components of the bacteriostatic preservative, and chlorhexidine acetate is a cationic surface active preservative, so that a novel preservative system is built in the system, and preservative components harmful to skin do not need to be additionally added.

3. The traditional Chinese medicine antibacterial components selected by the medical adhesive with high antibacterial performance comprise 1 part of semen euphorbiae lathyris extract, 2 parts of Yunnan buddleja extract, 2 parts of Japanese ampelopsis extract, 2 parts of liquorice extract and 1 part of gentiana macrophylla extract. The Euphorbiae Lathyridis semen extract contains diterpene alcohol and ester compounds, sterol compounds, coumarin compounds, and flavonoid compounds, and has strong antibacterial, antiinflammatory, analgesic, repercussive, astringent, and hemostatic effects. The plant of genus Buddleja contains more bioactive components including flavonoids, phenylpropanoid phenol glycoside, terpenoids, saponins, lignans and alkaloids, and has antibacterial, antiinflammatory, tranquilizing and analgesic effects. The radix Ampelopsis extract has effects of clearing heat and detoxicating, detumescence and resolving hard mass, promoting granulation and relieving pain, anti-inflammatory and antibacterial. The licorice extract contains liquiritin, liquiritigenin and polysaccharide which can effectively resist bacteria and improve inflammation and the like, and can effectively promote skin wound healing and improve scar conditions. The gentiana macrophylla has the effects of dispelling wind, clearing away heat and toxic materials, resisting inflammation, inhibiting bacteria, relieving pain and the like. The main components of the extract are iridoid glycosides, triterpenes, sterols and saccharides, which have good antibacterial property and can effectively promote wound healing and skin growth. The invention fully combines the main antibacterial action of the five traditional Chinese medicine substances, and simultaneously fully utilizes the anti-inflammatory, analgesic and hemostatic actions of the moleplant seed extract; the anti-inflammatory and analgesic effects of the extract of the Yunnan buddleja officinalis; radix Ampelopsis extract has effects of clearing heat and detoxicating, detumescence and resolving hard mass, promoting granulation and relieving pain; the Glycyrrhrizae radix extract has effects of promoting skin wound healing and improving scar condition, and radix Gentianae Marcrophyllae has effects of dispelling pathogenic wind and promoting wound healing and skin growth. The traditional Chinese medicine antibacterial component assists the specific nano antibacterial component and the natural antibacterial component to form three antibacterial systems, and simultaneously has the effects of diminishing inflammation, easing pain, dispelling wind, promoting wound healing and skin growth, so that the product can become a medical adhesive with comprehensive functions, which is more suitable for clinical operation. The invention combines the natural antibacterial component and the traditional Chinese medicine antibacterial component, and the advantages of the product are more prominent, because the biological transdermal enhancer is derived from plant extracts, the biological transdermal enhancer has good biocompatibility, can not cause damage to skin, and has safety.

4. Other components selected by the medical adhesive with high antibacterial performance have high-efficiency antibacterial function and become an indispensable component of the whole antibacterial product. The effects of various antibacterial components are natural, and the medical adhesive with high antibacterial performance is prepared. The poly (alpha-cyano isobutyl acrylate-alpha-cyano octyl acrylate) is used as a main raw material in a system, is prepared by an emulsification method, and has good biocompatibility and excellent antibacterial performance. The alpha-cyanoacrylate is sterile, and has high bacteriostatic effect on golden yellow staphylococcus, white staphylococcus, tetrabacillus, bacillus subtilis and the like. The polyisoprene is the main rubber component of the natural latex, has high purity and safety to human bodies, has good biocompatibility and good antibacterial performance, and is easy to be combined and fused with antibacterial materials, so that the antibacterial performance of the whole material is enhanced.

The tannic acid contains a unique polyphenol hydroxyl structure, can be combined with biological macromolecules such as protein polysaccharide and the like and can also generate complexation with metal ions, the polyphenol hydroxyl structure contains a catechol structure, so the tannic acid has good reducibility, astringency and antibacterial property, is clinically used for treating local bleeding, and can be used for synthesizing gallic acid and pyrogallol (pyrogallic acid and pyrogallol). Tannic acid is a main raw material for preparing an antibacterial synergist in the pharmaceutical industry.

The lignin has various functional groups and can be directly used as a curing agent, and the lignin is added into the resin to prepare the adhesive, so that the adhesive has good aging resistance and excellent antibacterial performance.

The oleum Hippophae is a complex of vitamins and bioactive substances. It has effects in nourishing skin, promoting metabolism, resisting allergy, resisting bacteria, relieving inflammation, promoting epithelial cell regeneration, repairing skin, maintaining skin acidic environment, and has strong permeability.

Sodium alginate is a natural polysaccharide carbohydrate, and a medical material prepared by taking alginate as a raw material has good affinity with wounds, has the characteristic of rapid hemostasis, can be used for repairing tissues and organs of a human body, and has antibacterial property.

The stabilizing agent selected by the invention adopts a composition of sulfone compounds, and the sulfone compounds are very effective antibacterial agents for a plurality of gram-positive and gram-negative bacteria.

The formaldehyde inhibitors selected in the present invention are benzoguanamine and hydantoin, which themselves have broad spectrum antimicrobial activity.

In conclusion, all the raw materials selected by the invention have antibacterial performance, three antibacterial systems of a specific nano antibacterial component, a natural antibacterial component and a traditional Chinese medicine antibacterial component are added on the basis of the antibacterial performance of the medical adhesive, so that the antibacterial performance of the medical adhesive is comprehensively improved, and the medical adhesive with high antibacterial performance prepared by the invention has stronger and more efficient antibacterial performance and complete comprehensive performance under the synergistic effect.

5. The formaldehyde inhibitor selected by the medical adhesive with high antibacterial performance is formed by combining benzoguanamine and hydantoin according to the weight percentage of 1:4, and the benzoguanamine and the hydantoin have broad-spectrum antibacterial activity, so that the formaldehyde can be inhibited by synergistic cooperation of the two components, and a power assisting effect can be provided for the antibacterial performance of the medical adhesive with high antibacterial performance. It should be further noted that the unique polyphenol hydroxyl structure of tannic acid in the present invention is an effective formaldehyde inhibitor, the sea buckthorn seed oil contains various active ingredients, wherein triterpenes are also effective formaldehyde inhibitors, the natural antibacterial component and the traditional Chinese medicine antibacterial component in the present invention contain triterpenes and sterols, which can be used as formaldehyde inhibitors. Namely, the invention is equivalent to that a mutually synergistic formaldehyde inhibition system is constructed in the raw materials of the invention, the formaldehyde inhibitor, the tannic acid, the natural antibacterial component and the traditional Chinese medicine antibacterial component which are selected by the invention are combined to play a role of efficiently inhibiting formaldehyde, and the medical adhesive of the invention is fully proved to have extremely low toxicity, greatly reduced use risk and more suitable for human bodies.

The medical adhesive with high antibacterial performance has the bonding strength of more than 60N, the antibacterial rate to staphylococcus aureus of more than 98.5 percent and the antibacterial rate to escherichia coli of 99 percent, greatly improves the overall healing efficiency, has high biocompatibility, no stimulation to wound tissues, reduces the sensitization rate, has excellent comprehensive performance, meets the application requirement of clinic on the medical adhesive, reduces the infection risk of patients to the maximum extent by improving the antibacterial performance of the medical adhesive, and provides guarantee for reaching higher cure rate.

Detailed Description

The invention is described in detail below by means of specific examples:

example 1

A medical adhesive with high antibacterial performance comprises the following components in parts by weight:

45 parts of poly (alpha-isobutyl cyanoacrylate-alpha-octyl cyanoacrylate), 24 parts of efficient antibacterial component, 2 parts of polyurethane, 20 parts of polyisoprene, 2 parts of sodium alginate, 3 parts of lignin, 7 parts of tannic acid, 1 part of stabilizer, 1 part of formaldehyde inhibitor, 150 parts of sea-buckthorn seed oil and 180 parts of sterile water; the high-efficiency antibacterial component comprises 5 parts of specific nano antibacterial component, 12 parts of natural antibacterial component and 5 parts of traditional Chinese medicine antibacterial component; the specific nano antibacterial component is formed by mixing silver-loaded nano titanium dioxide, chlorhexidine acetate-montmorillonite and graphene oxide according to the weight part ratio of 2:2: 1; the natural antibacterial component comprises 2 parts of a butterfly fruit extract, 2 parts of a aronia melanocarpa extract, 3 parts of a sweet wormwood herb extract, 2 parts of a mulberry bark extract, 2 parts of a citrus peel extract and 2 parts of a moso bamboo leaf extract in parts by weight; the traditional Chinese medicine antibacterial component comprises, by weight, 1 part of semen euphorbiae lathyris extract, 2 parts of Yunnan buddleja extract, 2 parts of radix ampelopsis extract, 2 parts of liquorice extract and 1 part of gentiana macrophylla extract. The stabilizer is formed by combining 3,3 '-diamino-4, 4' -dihydroxy diphenyl sulfone and dimethyl sulfolane according to the weight part ratio of 3: 1; the formaldehyde inhibitor is formed by combining benzoguanamine and hydantoin according to the weight percentage of 1: 4.

The medical adhesive prepared by the embodiment is used for smearing wounds, and relevant performance tests are carried out, wherein the test results are as follows: the medical adhesive with high antibacterial performance has the bonding strength of 62N, the antibacterial rate to staphylococcus aureus of more than 98.3 percent and the antibacterial rate to escherichia coli of 99 percent.

Example 2

A medical adhesive with high antibacterial performance comprises the following components in parts by weight:

50 parts of poly (alpha-isobutyl cyanoacrylate-alpha-octyl cyanoacrylate), 29 parts of efficient antibacterial component, 3 parts of polyurethane, 21 parts of polyisoprene, 3 parts of sodium alginate, 4 parts of lignin, 8 parts of tannic acid, 1 part of stabilizer, 1 part of formaldehyde inhibitor, 180 parts of sea-buckthorn seed oil and 180 parts of sterile water; the high-efficiency antibacterial component comprises 5 parts of specific nano antibacterial component, 14 parts of natural antibacterial component and 7 parts of traditional Chinese medicine antibacterial component; the specific nano antibacterial component is formed by mixing silver-loaded nano titanium dioxide, chlorhexidine acetate-montmorillonite and graphene oxide according to the weight part ratio of 2:2: 1; the natural antibacterial component comprises 2 parts of a butterfly fruit extract, 2 parts of a aronia melanocarpa extract, 3 parts of a sweet wormwood herb extract, 2 parts of a mulberry bark extract, 2 parts of a citrus peel extract and 2 parts of a moso bamboo leaf extract in parts by weight; the traditional Chinese medicine antibacterial component comprises, by weight, 1 part of semen euphorbiae lathyris extract, 2 parts of Yunnan buddleja extract, 2 parts of radix ampelopsis extract, 2 parts of liquorice extract and 1 part of gentiana macrophylla extract. The stabilizer is formed by combining 3,3 '-diamino-4, 4' -dihydroxy diphenyl sulfone and dimethyl sulfolane according to the weight part ratio of 3: 1; the formaldehyde inhibitor is formed by combining benzoguanamine and hydantoin according to the weight percentage of 1: 4.

The medical adhesive prepared by the embodiment is used for smearing wounds, and relevant performance tests are carried out, wherein the test results are as follows: the medical adhesive with high antibacterial performance has the bonding strength of 62.6N, the antibacterial rate to staphylococcus aureus of more than 98.5 percent and the antibacterial rate to escherichia coli of 99 percent.

Example 3

A medical adhesive with high antibacterial performance comprises the following components in parts by weight:

53 parts of poly (alpha-isobutyl cyanoacrylate-alpha-octyl cyanoacrylate), 32 parts of efficient antibacterial component, 4 parts of polyurethane, 23 parts of polyisoprene, 3 parts of sodium alginate, 5 parts of lignin, 9 parts of tannic acid, 1 part of stabilizer, 1 part of formaldehyde inhibitor, 190 parts of sea-buckthorn seed oil and 180 parts of sterile water; the high-efficiency antibacterial component comprises 5 parts of specific nano antibacterial component, 16 parts of natural antibacterial component and 9 parts of traditional Chinese medicine antibacterial component; the specific nano antibacterial component is formed by mixing silver-loaded nano titanium dioxide, chlorhexidine acetate-montmorillonite and graphene oxide according to the weight part ratio of 2:2: 1; the natural antibacterial component comprises 2 parts of a butterfly fruit extract, 2 parts of a aronia melanocarpa extract, 3 parts of a sweet wormwood herb extract, 2 parts of a mulberry bark extract, 2 parts of a citrus peel extract and 2 parts of a moso bamboo leaf extract in parts by weight; the traditional Chinese medicine antibacterial component comprises, by weight, 1 part of semen euphorbiae lathyris extract, 2 parts of Yunnan buddleja extract, 2 parts of radix ampelopsis extract, 2 parts of liquorice extract and 1 part of gentiana macrophylla extract. The stabilizer is formed by combining 3,3 '-diamino-4, 4' -dihydroxy diphenyl sulfone and dimethyl sulfolane according to the weight part ratio of 3: 1; the formaldehyde inhibitor is formed by combining benzoguanamine and hydantoin according to the weight percentage of 1: 4.

The medical adhesive prepared by the embodiment is used for smearing wounds, and relevant performance tests are carried out, wherein the test results are as follows: the medical adhesive with high antibacterial performance has the bonding strength of 63.3N, the antibacterial rate to staphylococcus aureus of more than 98.7 percent and the antibacterial rate to escherichia coli of 99 percent.

Example 4

A medical adhesive with high antibacterial performance comprises the following components in parts by weight:

55 parts of poly (alpha-isobutyl cyanoacrylate-alpha-octyl cyanoacrylate), 36 parts of efficient antibacterial component, 5 parts of polyurethane, 25 parts of polyisoprene, 4 parts of sodium alginate, 6 parts of lignin, 10 parts of tannic acid, 1 part of stabilizer, 1 part of formaldehyde inhibitor, 200 parts of sea buckthorn seed oil and 180 parts of sterile water; the high-efficiency antibacterial component comprises 5 parts of specific nano antibacterial component, 18 parts of natural antibacterial component and 10 parts of traditional Chinese medicine antibacterial component; the specific nano antibacterial component is formed by mixing silver-loaded nano titanium dioxide, chlorhexidine acetate-montmorillonite and graphene oxide according to the weight part ratio of 2:2: 1; the natural antibacterial component comprises 2 parts of a butterfly fruit extract, 2 parts of a aronia melanocarpa extract, 3 parts of a sweet wormwood herb extract, 2 parts of a mulberry bark extract, 2 parts of a citrus peel extract and 2 parts of a moso bamboo leaf extract in parts by weight; the traditional Chinese medicine antibacterial component comprises, by weight, 1 part of semen euphorbiae lathyris extract, 2 parts of Yunnan buddleja extract, 2 parts of radix ampelopsis extract, 2 parts of liquorice extract and 1 part of gentiana macrophylla extract. The stabilizer is formed by combining 3,3 '-diamino-4, 4' -dihydroxy diphenyl sulfone and dimethyl sulfolane according to the weight part ratio of 3: 1; the formaldehyde inhibitor is formed by combining benzoguanamine and hydantoin according to the weight percentage of 1: 4.

The medical adhesive prepared by the embodiment is used for smearing wounds, and relevant performance tests are carried out, wherein the test results are as follows: the medical adhesive with high antibacterial performance has the bonding strength of 64N, the antibacterial rate to staphylococcus aureus of more than 98.9 percent and the antibacterial rate to escherichia coli of 99 percent.

In the above embodiments

The specific extraction steps of the butterfly fruit extract are as follows: pulverizing dried semen Oroxyli, sieving with 60 mesh sieve, adding 18 times of 95% ethanol solution, soaking for 80 hr, filtering, and centrifuging at 3000rpm/min for 15 min; collecting supernatant, concentrating by rotary evaporation to concentration of 1.5g/ml, and sterilizing the concentrated solution with microporous membrane to obtain semen Oroxyli extract.

The preparation method of the sweet wormwood herb extract, the mulberry bark extract, the citrus peel extract and the moso bamboo leaf extract specifically comprises the following steps: drying the raw materials, respectively pulverizing, sieving with 100 mesh sieve to obtain powder, respectively, adding 95% ethanol solution, and ultrasonic extracting at 45 deg.C and 200W for 35min to obtain extractive solution; filtering the extractive solution, and concentrating under reduced pressure to obtain slurry; the slurry was mixed at a ratio of 1: dissolving the raw materials in deionized water at a volume ratio of 12, stirring, shaking, and sterilizing with microporous membrane filter to obtain the extracts.

The preparation method of the aronia melanocarpa extract comprises the following steps: washing mud on the surface of the aronia melanocarpa with flowing water, introducing an ozone generator into sterile water to wash the aronia melanocarpa again, crushing pulp into pulp, adding deionized water accounting for 40% of the volume of the pulp and pectinase 2.5%, uniformly stirring, and standing at 30 ℃ for 40 min; adding the supernatant into 95% ethanol solution, ultrasonic extracting at 40 deg.C and 200W ultrasonic power for 15min, and centrifuging at 4000rpm/min for 10 min; after concentrating the supernatant from the centrifugation to a semi-fluid slurry, the slurry was mixed at a ratio of 1: dissolving the extract in deionized water at a volume ratio of 20, stirring, shaking, and sterilizing with microporous membrane to obtain Aronia melanocarpa extract.

The concrete extraction steps of the moleplant seed extract, the yunnan buddleia extract, the ampelopsis japonica extract, the liquorice extract and the gentiana macrophylla extract are as follows: pulverizing dried semen Euphorbiae, Yunnan Buddleja, radix Ampelopsis, Glycyrrhrizae radix and radix Gentianae Marcrophyllae, sieving with 200 mesh sieve, adding 30 times of 90% ethanol solution, soaking for 75 hr, filtering, and centrifuging at 4000rpm/min for 12 min; collecting supernatant, concentrating by rotary evaporation to concentration of 0.8g/ml, and sterilizing the concentrated solution with microporous membrane filter to obtain Euphorbiae Lathyridis semen extract, YUNNANZUIYUCAO extract, radix Ampelopsis extract, Glycyrrhrizae radix extract and radix Gentianae Marcrophyllae extract.

The preparation method of the medical adhesive with high antibacterial performance comprises the following steps:

step one, adding sodium alginate, lignin, tannic acid and a stabilizer into sterile water according to the proportion of parts, heating to 100 ℃, stirring for dissolving for 150min, and cooling to room temperature to obtain an aqueous solution A;

step two, adding poly (alpha-cyano isobutyl acrylate-alpha-cyano octyl acrylate), polyurethane and polyisoprene into the sea buckthorn seed oil according to the parts ratio, heating to 80 ℃, stirring for 75min, and cooling to room temperature to obtain a sea buckthorn seed oil solution B;

step three, uniformly mixing the aqueous solution A and the seabuckthorn seed oil solution B, adding the efficient antibacterial component and the formaldehyde inhibitor according to the proportion by parts, and stirring for 4 hours;

and step four, filtering and sterilizing the product obtained in the step three, subpackaging, sealing and packaging to obtain the medical adhesive with high antibacterial performance.

Performance testing

The medical adhesive with high antibacterial performance has the bonding strength of more than 60N, the antibacterial rate to staphylococcus aureus of more than 98 percent and the antibacterial rate to escherichia coli of 99 percent, greatly reduces the risk of users, improves the whole healing efficiency, has good biocompatibility, no stimulation to wound tissues and excellent comprehensive performance, meets the application requirement of clinic on the medical adhesive, can reduce the infection risk of patients to the maximum extent by improving the antibacterial performance of the medical adhesive, and provides guarantee for reaching higher cure rate.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims (1)

1. A preparation method of a medical adhesive with high antibacterial performance comprises the following components in parts by weight: 45-55 parts of poly (alpha-isobutyl cyanoacrylate-alpha-octyl cyanoacrylate), 24-36 parts of efficient antibacterial component, 2-5 parts of polyurethane, 20-25 parts of polyisoprene, 2-4 parts of sodium alginate, 3-6 parts of lignin, 7-10 parts of tannic acid, 1 part of stabilizer, 1 part of formaldehyde inhibitor, 150 parts of sea-buckthorn seed oil and 180 parts of sterile water;

the high-efficiency antibacterial component comprises 5 parts of specific nano antibacterial component, 12-18 parts of natural antibacterial component and 5-10 parts of traditional Chinese medicine antibacterial component;

the specific nano antibacterial component is prepared by mixing silver-loaded nano titanium dioxide, chlorhexidine acetate-montmorillonite and graphene oxide according to the weight part ratio of 2:2: 1;

the natural antibacterial component comprises 2 parts of a butterfly fruit extract, 2 parts of a aronia melanocarpa extract, 3 parts of a sweet wormwood herb extract, 2 parts of a mulberry bark extract, 2 parts of a citrus peel extract and 2 parts of a moso bamboo leaf extract in parts by weight;

the traditional Chinese medicine antibacterial component comprises, by weight, 1 part of semen euphorbiae lantu extract, 2 parts of Yunnan buddleja extract, 2 parts of Japanese ampelopsis extract, 2 parts of liquorice extract and 1 part of gentiana macrophylla extract;

the method comprises the following steps:

step one, adding sodium alginate, lignin, tannic acid and a stabilizer into sterile water according to the proportion of parts, heating to 100 ℃, stirring for dissolving for 150min, and cooling to room temperature to obtain an aqueous solution A;

step two, adding poly (alpha-cyano isobutyl acrylate-alpha-cyano octyl acrylate), polyurethane and polyisoprene into the sea buckthorn seed oil according to the parts ratio, heating to 80 ℃, stirring for 75min, and cooling to room temperature to obtain a sea buckthorn seed oil solution B;

step three, uniformly mixing the aqueous solution A and the seabuckthorn seed oil solution B, adding the efficient antibacterial component and the formaldehyde inhibitor according to the proportion by parts, and stirring for 4 hours;

and step four, filtering and sterilizing the product obtained in the step three, subpackaging, sealing and packaging to obtain the medical adhesive with high antibacterial performance.