CN108186430A - Sebum film, the mung bean zymotic fluid for soothing the skin allergy and preparation method thereof are damaged with repairing - Google Patents
Sebum film, the mung bean zymotic fluid for soothing the skin allergy and preparation method thereof are damaged with repairing Download PDFInfo
- Publication number
- CN108186430A CN108186430A CN201711260225.0A CN201711260225A CN108186430A CN 108186430 A CN108186430 A CN 108186430A CN 201711260225 A CN201711260225 A CN 201711260225A CN 108186430 A CN108186430 A CN 108186430A
- Authority
- CN
- China
- Prior art keywords
- mung bean
- skin
- zymotic fluid
- filtrate
- skin allergy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 240000004922 Vigna radiata Species 0.000 title claims abstract description 44
- 235000010721 Vigna radiata var radiata Nutrition 0.000 title claims abstract description 44
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 title claims abstract description 44
- 239000012530 fluid Substances 0.000 title claims abstract description 22
- 210000002374 sebum Anatomy 0.000 title claims abstract description 20
- 201000004624 Dermatitis Diseases 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 20
- 238000000855 fermentation Methods 0.000 claims abstract description 19
- 230000001771 impaired effect Effects 0.000 claims abstract description 17
- 230000004151 fermentation Effects 0.000 claims abstract description 16
- 239000000706 filtrate Substances 0.000 claims abstract description 15
- 241000894006 Bacteria Species 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 239000004310 lactic acid Substances 0.000 claims abstract description 10
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 10
- 239000011573 trace mineral Substances 0.000 claims abstract description 10
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 10
- 239000008267 milk Substances 0.000 claims abstract description 9
- 235000013336 milk Nutrition 0.000 claims abstract description 9
- 210000004080 milk Anatomy 0.000 claims abstract description 9
- 239000007787 solid Substances 0.000 claims abstract description 9
- 238000012545 processing Methods 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 238000000265 homogenisation Methods 0.000 claims abstract description 4
- 238000005374 membrane filtration Methods 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims abstract description 3
- 239000000047 product Substances 0.000 claims description 13
- LQIAZOCLNBBZQK-UHFFFAOYSA-N 1-(1,2-Diphosphanylethyl)pyrrolidin-2-one Chemical compound PCC(P)N1CCCC1=O LQIAZOCLNBBZQK-UHFFFAOYSA-N 0.000 claims description 8
- 239000002002 slurry Substances 0.000 claims description 7
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000000284 extract Substances 0.000 claims description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 3
- 239000011575 calcium Substances 0.000 claims description 3
- 229910052791 calcium Inorganic materials 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 239000011701 zinc Substances 0.000 claims description 3
- 229910052725 zinc Inorganic materials 0.000 claims description 3
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims description 2
- 230000018044 dehydration Effects 0.000 claims description 2
- 238000006297 dehydration reaction Methods 0.000 claims description 2
- 229910052732 germanium Inorganic materials 0.000 claims description 2
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 claims description 2
- 238000005286 illumination Methods 0.000 claims description 2
- 229910052744 lithium Inorganic materials 0.000 claims description 2
- 239000011777 magnesium Substances 0.000 claims description 2
- 229910052749 magnesium Inorganic materials 0.000 claims description 2
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 229910052711 selenium Inorganic materials 0.000 claims description 2
- 239000011669 selenium Substances 0.000 claims description 2
- 229910052720 vanadium Inorganic materials 0.000 claims description 2
- LEONUFNNVUYDNQ-UHFFFAOYSA-N vanadium atom Chemical compound [V] LEONUFNNVUYDNQ-UHFFFAOYSA-N 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 17
- 230000002401 inhibitory effect Effects 0.000 abstract description 10
- 230000000638 stimulation Effects 0.000 abstract description 8
- 108010003272 Hyaluronate lyase Proteins 0.000 abstract description 7
- 102000001974 Hyaluronidases Human genes 0.000 abstract description 7
- 229960002773 hyaluronidase Drugs 0.000 abstract description 7
- 206010039580 Scar Diseases 0.000 abstract description 3
- 230000019612 pigmentation Effects 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 56
- 230000003266 anti-allergic effect Effects 0.000 description 19
- 206010020751 Hypersensitivity Diseases 0.000 description 14
- 239000010408 film Substances 0.000 description 13
- 208000002874 Acne Vulgaris Diseases 0.000 description 12
- 206010000496 acne Diseases 0.000 description 12
- 238000012360 testing method Methods 0.000 description 10
- 208000026935 allergic disease Diseases 0.000 description 9
- 230000007815 allergy Effects 0.000 description 8
- 230000035945 sensitivity Effects 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 210000003743 erythrocyte Anatomy 0.000 description 7
- 239000002994 raw material Substances 0.000 description 7
- 230000002829 reductive effect Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 230000003110 anti-inflammatory effect Effects 0.000 description 5
- 230000006378 damage Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 208000024891 symptom Diseases 0.000 description 5
- 206010018910 Haemolysis Diseases 0.000 description 4
- 208000030961 allergic reaction Diseases 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 229930003935 flavonoid Natural products 0.000 description 4
- 150000002215 flavonoids Chemical class 0.000 description 4
- 235000017173 flavonoids Nutrition 0.000 description 4
- 230000008588 hemolysis Effects 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 235000018553 tannin Nutrition 0.000 description 4
- 229920001864 tannin Polymers 0.000 description 4
- 239000001648 tannin Substances 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 210000003630 histaminocyte Anatomy 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- 208000014674 injury Diseases 0.000 description 3
- 150000008442 polyphenolic compounds Chemical class 0.000 description 3
- 235000013824 polyphenols Nutrition 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 230000024053 secondary metabolic process Effects 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 230000004936 stimulating effect Effects 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000186427 Cutibacterium acnes Species 0.000 description 2
- BIVBRWYINDPWKA-VLQRKCJKSA-L Glycyrrhizinate dipotassium Chemical compound [K+].[K+].O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@H]1CC[C@]2(C)[C@H]3C(=O)C=C4[C@@H]5C[C@](C)(CC[C@@]5(CC[C@@]4(C)[C@]3(C)CC[C@H]2C1(C)C)C)C(O)=O)C([O-])=O)[C@@H]1O[C@H](C([O-])=O)[C@@H](O)[C@H](O)[C@H]1O BIVBRWYINDPWKA-VLQRKCJKSA-L 0.000 description 2
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 208000003251 Pruritus Diseases 0.000 description 2
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 2
- 206010070834 Sensitisation Diseases 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000002052 anaphylactic effect Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 239000000022 bacteriostatic agent Substances 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 238000005842 biochemical reaction Methods 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 229940101029 dipotassium glycyrrhizinate Drugs 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 210000004209 hair Anatomy 0.000 description 2
- 230000009610 hypersensitivity Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000002757 inflammatory effect Effects 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 230000007794 irritation Effects 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 210000002540 macrophage Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 229940055019 propionibacterium acne Drugs 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000037307 sensitive skin Effects 0.000 description 2
- 230000037380 skin damage Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 150000003431 steroids Chemical class 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 206010067484 Adverse reaction Diseases 0.000 description 1
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 206010015150 Erythema Diseases 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 206010020880 Hypertrophy Diseases 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 102000004889 Interleukin-6 Human genes 0.000 description 1
- 108090001005 Interleukin-6 Proteins 0.000 description 1
- 102000004890 Interleukin-8 Human genes 0.000 description 1
- 108090001007 Interleukin-8 Proteins 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 206010033733 Papule Diseases 0.000 description 1
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 1
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 206010042496 Sunburn Diseases 0.000 description 1
- 230000006052 T cell proliferation Effects 0.000 description 1
- 206010043189 Telangiectasia Diseases 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 208000000260 Warts Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000003255 anti-acne Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000001139 anti-pruritic effect Effects 0.000 description 1
- 239000000043 antiallergic agent Substances 0.000 description 1
- 239000003908 antipruritic agent Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000003822 cell turnover Effects 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000036570 collagen biosynthesis Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 244000013123 dwarf bean Species 0.000 description 1
- 210000004177 elastic tissue Anatomy 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 210000003780 hair follicle Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036074 healthy skin Effects 0.000 description 1
- 229960001340 histamine Drugs 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000000893 inhibin Substances 0.000 description 1
- ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 229940100601 interleukin-6 Drugs 0.000 description 1
- 229940096397 interleukin-8 Drugs 0.000 description 1
- XKTZWUACRZHVAN-VADRZIEHSA-N interleukin-8 Chemical compound C([C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@@H](NC(C)=O)CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCSC)C(=O)N1[C@H](CCC1)C(=O)N1[C@H](CCC1)C(=O)N[C@@H](C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC=1C=CC(O)=CC=1)C(=O)N[C@H](CO)C(=O)N1[C@H](CCC1)C(N)=O)C1=CC=CC=C1 XKTZWUACRZHVAN-VADRZIEHSA-N 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 1
- 229920006008 lipopolysaccharide Polymers 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 150000003180 prostaglandins Chemical class 0.000 description 1
- 235000005875 quercetin Nutrition 0.000 description 1
- 229960001285 quercetin Drugs 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 230000008591 skin barrier function Effects 0.000 description 1
- 201000010153 skin papilloma Diseases 0.000 description 1
- 230000036548 skin texture Effects 0.000 description 1
- 210000003859 smegma Anatomy 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 210000000106 sweat gland Anatomy 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 208000009056 telangiectasis Diseases 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/004—Aftersun preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/007—Preparations for dry skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/75—Anti-irritant
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Dermatology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Chemical & Material Sciences (AREA)
- Birds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of mung bean zymotic fluid for having and repairing impaired sebum film, soothing the skin allergy, comprising by Mung Bean, through broken wall filtrate obtained by lactobacillus-fermented, solid content is 1~20% in filtrate；The invention also discloses a kind of preparation methods for having and repairing impaired sebum film, the mung bean zymotic fluid for soothing the skin allergy, and its step are as follows：1) Mung Bean；2) lactic acid bacteria breeding；3) Mung Bean carries out liquid homogenate, and adds milk and trace element, finally carries out steam sterilizing, cooling；4) strain fermentation is connect；5) high-pressure homogenization broken wall；6) freeze concentration, coarse filtration processing；7) membrane filtration bacteria removing；For the present invention to carry out lactobacillus-fermented after rudiment at the beginning of mung bean seed, gained broken wall filtrate is fool proof, and no any external application stimulation has the effect of significantly inhibiting hyaluronidase, quickly eliminates Propiobacterium, reduce pigmentation, inhibits acne scars generation.
Description
Technical field
The invention belongs to the technical field characterized by component, be specifically related to it is a kind of have repair impaired sebum film,
Soothe the skin mung bean zymotic fluid of allergy and preparation method thereof.
Background technology
Why skin can keep flexible smooth, be primarily due in skin histology containing a certain amount of moisture, in addition skin
The grease of smegma in skin and the sweat of sweat gland secretion mix, and one layer of very thin film is formed in skin surface,
Medically it is referred to as " sebum film ", it can moisten skin and the evaporation that greatly reduces moisture in skin histology；Once skin
Adipose membrane is destroyed, and not only water retaining function reduces, and skin can also be made to become drying, and itch is even casted off a skin；To factors such as weathers
Reagency also weakens therewith, easily causes skin red and swollen, part is general red or even sensitivity phenomenon occurs.It is particularly easy to pigment occur to sink
It forms sediment, skin is not pale enough.Can also feel after face cleaning skin than it is healthy when it is dry and astringent tight.Society now, with environmental pollution and
The chemicals of contact is continuously increased, and is destroyed skin sebum film, and various sensitivity skin and allergy skin are continuously increased；
Allergy is body (immunocompetence is low) under the Oxidative demage effect of a large amount of free radicals, makes mast cell and basophilic
Granulocyte is unstable, denaturation, after the stimulation of anaphylactogen, leads to membranolysis, caused by the release of sensitization medium；Medicine is demonstrate,proved
Real, the allergy of more than 90% recurrent exerbation is to be used for a long time the cosmetics that imply hormone or other stimulating components, allergy it
After reuse steroids antiallergic product, leading to skin, allergy forms sensitive skin repeatedly, finally occur steroid dependent dermatitis,
Red blood silk etc.；
Substantially most of for mung bean extraction process at present is all to use physics, chemical extracting method, in extraction process
There are problems that extraction efficiency is low, organic chemicals residual is difficult to remove.
Invention content
In view of this, the purpose of the present invention is to provide it is a kind of have repair impaired sebum film, soothe the skin the green of allergy
Beans zymotic fluid and preparation method thereof, a kind of mung bean seed rudiment lactobacillus-fermented product after rudiment at the beginning of mung bean seed to carry out lactic acid bacteria
Fermentation, gained broken wall filtrate is fool proof, and no any external application stimulation has the effect of significantly inhibiting hyaluronidase, quickly disappears
Except Propiobacterium, pigmentation is reduced, inhibits acne scars generation.
A kind of mung bean zymotic fluid preparation method for having the impaired sebum film of reparation, soothing the skin allergy utilizes mung bean seed to pass through
After crossing bioactivation rudiment, using a kind of biological lactic acid strain fermentation secretion inducing, physiological is gone out by biologically pure after broken bacterium
Active small peptide, exocellular polysaccharide (EPS) and lysate in confrontation scytitis, releive skin and reparation skin barrier, are protected
Shield cell is not damaged, and has outstanding representation in terms of confrontation Propiobacterium and moisturizing, confrontation skin ageing.
In order to achieve the above object, the present invention is a kind of has the mung bean fermentation repaired impaired sebum film, soothe the skin allergy
Liquid, comprising through broken wall filtrate obtained by lactobacillus-fermented, solid content is 1~20% in filtrate by Mung Bean.
Further, solid content is set as 5~10% in the filtrate.
Further, in the filtrate containing trace element, it is described trace element include calcium, magnesium, iron, zinc, potassium, manganese, lithium,
Germanium, vanadium and selenium.
The invention also discloses a kind of preparation sides for having and repairing impaired sebum film, the mung bean zymotic fluid for soothing the skin allergy
Method, its step are as follows：
1) mung bean seed deionized water is cleaned, impregnated 6~12 hours at room temperature, illumination cultivation is carried out after dehydration, at 22 DEG C
It is cultivated 12~18 hours under~25 DEG C of constant temperature；
2) lactic acid bacteria is cultivated, its strain density is made to reach inoculation requirement, treats that fermentation uses；
3) rudiment product in step 1) is subjected to liquid homogenate, and adds milk and trace element, then carried out steam and go out
Bacterium is finally cooled to 38 DEG C~42 DEG C；
4) it by the strain in step 2), proportionally adds in the slurries of step 3), carries out anaerobic fermentation；
5) fermentation finishes, and carries out high-pressure homogenization broken wall treatment；
6) broken wall treatment treated slurries are subjected to freeze concentration processing, slurries are made to keep solid content more than 5.0%,
Then it is filtered bulk processing；
7) liquid of coarse filtration is subjected to membrane filtration degerming packing, obtains mung bean seed extract and the filter of lactobacillus-fermented product
Liquid.
The beneficial effects of the present invention are：
1st, the mung bean zymotic fluid of the invention for having the impaired sebum film of reparation, soothing the skin allergy is green with foolproof food materials
Lactobacillus-fermented is carried out after the just rudiment of beans seed, gained broken wall filtrate is fool proof, and no any external application stimulation has and significantly inhibits
The effect of bright matter acid enzyme, zymotic fluid can individually either compounding be made and releives or counter-stimulus, and stability is very good, performance
Go out excellent rat peritoneal mast cells degranulation inhibiting rate and rat hypertrophy cell histamine release inhibiting rate, and to skin without appointing
What adverse reaction exists；
2nd, the mung bean zymotic fluid of the invention for having the impaired sebum film of reparation, soothing the skin allergy is used to prepare acne vulgaris people
Treatment, nursing and the reparation of group's skin, can quickly eliminate Propiobacterium, reduce pigmentation, inhibit acne scars generation, i.e.,
Damaged skin is used in also to generate without excitement；The product of the present invention, which avoids traditional acne treatment and reparation, to be needed to rely on
Antibiotic, hormone and some chemical bacteriostatic agents, so as to avoid to the huge injury of human skin and accordance with tolerance, while the present invention adopts
It is substantially reduced with slow-releasing system technology and stimulation possibility is generated to human skin, increase the duration of action effect；
3rd, the mung bean zymotic fluid preparation method of the invention for having the impaired sebum film of reparation, soothing the skin allergy being capable of maximum limit
The biological activity that degree ground is preserved in product is not lost, and biological fermentation process generates new active substance, and extraction conditions are mild, have
It is high to imitate composition dissolution rate, no oxious component addition；
4th, the mung bean zymotic fluid preparation method of the invention for having the impaired sebum film of reparation, soothing the skin allergy, which belongs to, utilizes safety
Edible raw material mung bean seed carries out biofermentation and prepares raw material field, and bacteriostatic peptide is generated after fermenting by biological Induction Control
With active reparative factor, reparation or auxiliary available for problem skin are repaired, and wherein problem skin includes acne skin, red blood
Silk skin sensitivity skin, stimulated skin, dry exfoliated skin, sunburn skin etc.；
5th, the present invention can be used for preparing the drug or cosmetics that soothe the skin allergy and stimulation with present invention gained raw material,
It is safely and effectively non-stimulated, it avoids using chemical anti-sensitizer and plant extract agent for antiallergic to human injury and stimulation.
6th, biological synergetic antibacterial action is mainly come to acne treatment based on present invention utilization present invention preparation product
And directly to the effect of hyperkeratosis hair follicle as a result, then coming from making the repair cell factor in formula to skin damage reparation
With.
7th, product of the invention avoids traditional acne treatment and reparation and needs by antibiotic, hormone and some chemistry
Bacteriostatic agent, so as to avoid to the huge injury of human skin and dependence, while the present invention is dropped significantly using slow-releasing system technology
It is low that stimulation possibility is generated to human skin, increase the duration of action effect.
8th, present invention is mainly used for the treatment and reparation of human body acne vulgaris, such as acne, papule, warts, tubercle, sheets
Invention builds healthy skin immune system in medicine level, is not skin merely for the treatment and reparation of human body acne vulgaris
Skin surface is antipruritic, anti-irritant, and more theoretical critical should be prevention and the impaired skin epidermis of reparation, build the skin of health
Barrier effectively resists foreign matter infringement skin.
9th, the present invention, as substrate, utilizes biochemical reaction complicated during rudiment by the use of with rudiment mung bean when controlling
Its natural active matter is carried out fully to activate and convert；During control after rudiment, we exist with band plumule mung bean seed mixing milk
Under a kind of lactic acid bacteria effect, amphimicrobian fermentation is carried out, reduces protein in mung bean seed and milk, it is each to generate human skin needs
Kind amino acid, while generate the abundant secondary metabolism product such as Flavonoids, tannin class, Polyphenols.
10th, the antiallergic rhzomorph in the present invention has benefited from following aspect in antiallergic, the good behaviour repaired, releived：
Skin is anti-inflammatory disappear it is quick：There are natural active matter activation and transformation stages in Mung Bean；In germinating, due to new
The needs of old metabolism can release some biological activity enzyme and be activated so as to which biochemical reaction occur, under the action of complicated enzyme system,
The secondary metabolisms product such as biosynthesis flavonoids, rutin and Quercetin, Polyphenols；Mung bean embryo seed is in lactobacillus-fermented mistake simultaneously
Tannin class object is released in journey；Medicine confirms：Flavonoids, tannin constituents generally have anti-inflammatory effect, and Anti-inflammatory Mechanism is as follows：
1) by reducing t cell proliferation, anaphylactic antiboby IgE levels are reduced, so as to reduce I type hypersensitivity in sensitisation phase
Sensibility；
2) inhibit mast cell degranulation, reduce the active medium release in granule, control telangiectasis, blood vessel
Permeability increases, mucous membrane swelling etc., and so as to alleviate clinical symptoms, the effect of I type hypersensitivity is reduced in excitation phase；Pass through
Inhibit the release of the deposit substance and active intermediate medium in body allergic reaction, so as to reduce allergic reaction in the effective stage；
3) various nutriments is promoted to be significantly increased, if vitamin C starts to synthesize, the trace elements such as iron, calcium, zinc, copper contain
Amount increases.
Description of the drawings
Fig. 1 is mung bean zymotic fluid equivalent test chart of the present invention；
Fig. 2 is mung bean zymotic fluid biology equivalent test schematic device of the present invention；
Fig. 3 is mung bean zymotic fluid fibroblast design sketch of the present invention；
Fig. 4 is mung bean zymotic fluid liquid glue protoplasm synthetic effect figure of the present invention.
Specific embodiment
The preparation method that the present invention has the impaired sebum film of reparation, the mung bean zymotic fluid for soothing the skin allergy utilizes mung bean seed
With significant anti-inflammatory activity, lipopolysaccharides stimulating expression of macrophage process can be inhibited, so as to reach inhibition and allergic reaction phase
The generation of release and the inflammatory reaction of the medium (cell factor, prostaglandin) of pass utilizes bioid complicated during rudiment
Reaction is learned its natural active matter is carried out fully to activate and convert；During control after rudiment, we are with the seed mixing of band plumule mung bean
Milk carries out amphimicrobian fermentation in the case where a kind of lactic acid bacteria acts on, and reduces protein in mung bean seed and milk, generates human skin
Various amino acid are needed, while generate the abundant secondary metabolism product such as Flavonoids, tannin class, Polyphenols；Mung bean seed is former
Material itself still remains abundant starch and trace element after bioactivation handles rudiment, can be as excellent fermentation substrate
Milk is coordinated to carry out a kind of lactic bacteria strain fermentation by induction, secrets out of physiological activities object, EPS and lysate；Wherein
Secretion activity object exocellular polysaccharide (EPS) is present in body by the generation and competitive antagonism of stimulating cytokine in fermentation, exempts from
Epidemic disease inhibiting factor increases skin immune function, improves macrophage phagocytic activity and induced cell growth inhibin, interferon
With the generation of il-1 α.
Embodiment one, anti-inflammatory effect experiment (inhibiting interleukin in 24 hours)：It is biology equivalent test figure as shown in Figure 1
Table, biology equivalent test show, 2.0% mung bean streptococcus acidi lactici fermented solution is to leading to the inflammatory factor of horn cell hyperplasia
(interleukin-6), degree of suppression 85%；To leading to rubescent inflammatory factor (interleukin-8), degree of suppression is up to 90%；
Embodiment two：Inhibit hyaluronidase activity embodiment
Hyaluronidase is anaphylactoid participant, research shows that hyaluronidase and inflammation, the extremely strong phase of allergic reaction
Guan Xing inhibits embodiment (Elson-Morgan) method using hyaluronidase in vitro, as shown in the table：Test result shows institute
Show, using 2.0% dipotassium glycyrrhizinate as positive control, sample I (solid content 5.0%), take its 2.0% when inhibiting rate and positive control
It is about high, 95% is can reach, sample I (solid content 5.0%).
Data are shown：By the way that hyaluronidase activity is inhibited to test, antiallergic rhzomorph is in antiallergic effect, with dipotassium glycyrrhizinate
Effect is suitable, even more significantly.
Embodiment three：RBC haemolysis embodiments
Erythrocyte (RBC) is made using fresh ox blood, raw material hemolysis rate itself is measured with this and confrontation SDS haemolysis inhibits
Rate；Embodiment result is as follows：
1) antiallergic rhzomorph self-dissolving control group result：Its 5.0% (mass percent) antiallergic rhzomorph aqueous solution is in system, not
Erythrocyte haemolysis occurs, it was demonstrated that this raw material does not have to make the apparent self-dissolving of erythrocyte, shows that this raw material itself does not have
Obvious irritation.
2) antiallergic rhzomorph causes SDS the inhibiting rate of damage erythrocyte, adds 2.0% (mass percent)) antiallergic rhzomorph exists
Embodiment result shows that inhibiting rate reaches 46.7% in system, confirms that antiallergic rhzomorph effectively reduces SDS to erythrocyte with this
Damage, can determine that significantly reduce potential stimulus of the stimulant to skin.
Example IV：Sensitivity is repaired, and general red allergic phenomena is reduced/reduce as standard using values
At 8 tested men and women's ages 20-45 Sui, test method is as follows：
1st, patch irritation test (repeated adhering makes skin damage),
2nd, left hand smears basic emulsion, and the right hand smears+3% antiallergic rhzomorph of basic emulsion
3rd, subject cleans after 15 minutes, observation of taking pictures
Result of the test：After 3.0% antiallergic rhzomorph 15 minutes, contrast test is shown to be had not using antiallergic rhzomorph left hand
General red phenomenon is reduced with degree, analysis show that more than 50% mitigates；The right hand of basic emulsion, general red phenomenon is still apparent, without bright
It is aobvious to improve.
Embodiment five：Inhibit Propiobacterium
Microorganism bacteriostatic test：Observing samples are anti-so as to reflect to the inhibition zone sizes of propionibacterium acnes on tablet
Quick rhzomorph is to the inhibition of Propiobacterium：
As shown in Figure 2：Embodiment uses 3.0% concentration of antiallergic rhzomorph, sets three Duplicate Samples, can through antibacterial embodiment
To find out, green bean sprout extract (1,3, No. 5) has apparent bacteriostasis efficacy, Duplicate Samples (2,4,6 to propionibacterium acnes
Number) do not show have apparent inhibition zone to Propiobacterium.
Embodiment six：Volunteer 38 is selected, is sensitive skin quality, there are allergies, and its face is with acne
Symptom；Men and women is unlimited, carries out on probation and clinical observation, is randomly divided into 2 groups, every group 19：The clinical main performance of skin before observation
For skin sensitivity, itch is uncomfortable, the long-term breaking-out having:
1) implementation：
1 group：Daily after face cleaning, on skin and easily hair position 3.0% lotion of smearing preparation is appropriate, once in the morning and once at night；
2 groups (control groups)：After daily face cleaning, fitted on skin with easily hair position certain commercially available famous brand name anti-acne lotion of smearing
Amount；
Once in the morning and once at night.3 days, 7 days, observation in 28 days and statistical result.
2) judgment criteria：
(1) zero is cured：Clinical acne symptom disappears, is reacted again without skin sensitivity in one month；
(2) effective ※：Clinical acne symptom mitigates, skin sensitivity reactive moieties subside or disappears, and reduces；
(3) invalid X：Clinical acne symptom, skin sensitivity do not improve.
3) result on trial：It is as shown in the table：
As a result it shows：Antiallergic rhzomorph has Propiobacterium strong inhibiting effect, has effects that good Shu Min antiallergics, to Cuo
The skin of sore patient has stable repairing effect.
Embodiment seven：It is anti-aging
Increase with the age, cell turnover speed slows down, and the skin texture of skin can be improved at all by improving cell viability, improve squamous
Skin.As shown in Figure 3, Figure 4, antiallergic rhzomorph can assist improving cell activity, improve fibroblast proliferation rate, excessively
The horn cell of aging comes off, and increases collagen biosynthesis efficiency, makes the water content of skin corium increase, intradermal mucopolysaccharide,
Collagenous fibres, elastic fibers quantity increase, and skin are made to become beautiful, gloss, wrinkle gradually subsides.
Embodiment eight：The manufacturing process of mung bean antiallergic rhzomorph is as follows：
1) raw material sterilizes：After mung bean seed is sterilized, deionized water cleaning is carried out, is impregnated 6~12 hours at room temperature；
2) rudiment culture：Liquid shear sterilizes；It cultivates 12~18 hours under 22 DEG C~25 DEG C constant temperature, then produces rudiment
Object carries out liquid homogenate, and adds milk and trace element, then carries out steam sterilizing, is finally cooled to 38 DEG C~42 DEG C；
3) actication of culture：Lactic acid bacteria is cultivated, its strain density is made to reach inoculation requirement, treats that fermentation uses；
4) fermented and cultured：Lactic acid bacteria culturers are proportionally added in the slurries of step 2), carry out anaerobic fermentation；
5) bacterium processing is broken：Fermentation finishes, and carries out high-pressure homogenization broken wall treatment；
6) miillpore filter degerming：The liquid of coarse filtration is subjected to membrane filtration degerming packing, obtains mung bean seed extract and lactic acid
Bacterium tunning filtrate.
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
It crosses above preferred embodiment the present invention is described in detail, however, those skilled in the art should understand that, can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (4)
1. a kind of have the mung bean zymotic fluid repaired impaired sebum film, soothe the skin allergy, it is characterised in that：Comprising being sprouted by mung bean
Bud is through broken wall filtrate obtained by lactobacillus-fermented, and solid content is 1~20% in filtrate.
2. according to claim 1 have the mung bean zymotic fluid repaired impaired sebum film, soothe the skin allergy, feature exists
In：Solid content is set as 5~10% in the filtrate.
3. according to claim 1 have the mung bean zymotic fluid repaired impaired sebum film, soothe the skin allergy, feature exists
In：Include calcium, magnesium, iron, zinc, potassium, manganese, lithium, germanium, vanadium and selenium containing trace element, the trace element in the filtrate.
4. a kind of mung bean zymotic fluid that there is the impaired sebum film of reparation, soothe the skin allergy prepared described in claims 1 to 3
Preparation method, it is characterised in that：Its step are as follows：
1) mung bean seed deionized water is cleaned, impregnated 6~12 hours at room temperature, illumination cultivation is carried out after dehydration, 22 DEG C~25
It is cultivated 12~18 hours under DEG C constant temperature；
2) lactic acid bacteria is cultivated, its strain density is made to reach inoculation requirement, treats that fermentation uses；
3) rudiment product in step 1) is subjected to liquid homogenate, and adds milk and trace element, then carry out steam sterilizing, most
Postcooling is to 38 DEG C~42 DEG C；
4) it by the strain in step 2), proportionally adds in the slurries of step 3), carries out anaerobic fermentation；
5) fermentation finishes, and carries out high-pressure homogenization broken wall treatment；
6) broken wall treatment treated slurries are subjected to freeze concentration processing, slurries are made to keep solid content more than 5.0%, then
It is filtered bulk processing；
7) liquid of coarse filtration is subjected to membrane filtration degerming packing, obtains mung bean seed extract and lactobacillus-fermented Product filtrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711260225.0A CN108186430A (en) | 2017-12-04 | 2017-12-04 | Sebum film, the mung bean zymotic fluid for soothing the skin allergy and preparation method thereof are damaged with repairing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711260225.0A CN108186430A (en) | 2017-12-04 | 2017-12-04 | Sebum film, the mung bean zymotic fluid for soothing the skin allergy and preparation method thereof are damaged with repairing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108186430A true CN108186430A (en) | 2018-06-22 |
Family
ID=62573583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711260225.0A Pending CN108186430A (en) | 2017-12-04 | 2017-12-04 | Sebum film, the mung bean zymotic fluid for soothing the skin allergy and preparation method thereof are damaged with repairing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108186430A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108703924A (en) * | 2018-07-12 | 2018-10-26 | 佛山市汇汾化妆品科技有限公司 | A kind of plant Shu Min Essences and preparation method thereof |
| CN108785429A (en) * | 2018-07-31 | 2018-11-13 | 王世东 | A kind of external medicine composition and preparation method thereof repaired that has effects that releive |
| CN109481391A (en) * | 2018-12-04 | 2019-03-19 | 福建植护网络科技有限公司 | A kind of Baby Care cream and preparation method thereof compounding silver ion and green phenol song |
| CN109806175A (en) * | 2019-02-20 | 2019-05-28 | 高一铷 | A kind of addition moisturizing is releived gel combination |
| CN111991316A (en) * | 2020-09-15 | 2020-11-27 | 广州莱约生物科技有限公司 | Anti-allergy repairing composition and preparation method and application thereof |
| CN112516018A (en) * | 2019-09-18 | 2021-03-19 | 广州悦荟化妆品有限公司 | Selenium-rich mung bean fermentation liquor with whitening, anti-aging, moisturizing and toxin-expelling effects and mask composition thereof |
| CN115006310A (en) * | 2022-07-05 | 2022-09-06 | 菏泽市亿鑫生物科技有限公司 | Mung bean sprout fermentation product, skin external preparation containing mung bean sprout fermentation product, and preparation method and application of mung bean sprout fermentation product |
| CN115040449A (en) * | 2022-07-08 | 2022-09-13 | 广州悦荟化妆品有限公司 | Mung bean fermentation liquor with anti-inflammatory, relieving and repairing effects, mixed fermentation liquor, and preparation method and application thereof |
| CN115089529A (en) * | 2022-08-03 | 2022-09-23 | 肽源（广州）生物科技有限公司 | Mung bean hull fermentation product with acne removing effect and preparation method and application thereof |
| CN115836997A (en) * | 2022-11-02 | 2023-03-24 | 菏泽市亿鑫生物科技有限公司 | Mung bean seed fermented product, skin external preparation containing mung bean seed fermented product, and preparation method and application of mung bean seed fermented product |
| CN117757848A (en) * | 2023-12-29 | 2024-03-26 | 山东福瑞达生物科技有限公司 | A kind of lactic acid bacteria fermentation product and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102112106A (en) * | 2008-07-31 | 2011-06-29 | 高丽雅娜化妆品股份有限公司 | Cosmetic composition for anti-aging of the skin comprising phaseolus radiatus seed extracts by fermentation and enzyme treatment |
| CN102988242A (en) * | 2012-12-07 | 2013-03-27 | 上海相宜本草化妆品股份有限公司 | Method for preparing mung bean sprout extracting solution and application of mung bean sprout extracting solution |
| CN106265430A (en) * | 2016-08-22 | 2017-01-04 | 彭之玉 | Semen phaseoli radiati ferment licorice peace skin frost and preparation method |
-
2017
- 2017-12-04 CN CN201711260225.0A patent/CN108186430A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102112106A (en) * | 2008-07-31 | 2011-06-29 | 高丽雅娜化妆品股份有限公司 | Cosmetic composition for anti-aging of the skin comprising phaseolus radiatus seed extracts by fermentation and enzyme treatment |
| CN102988242A (en) * | 2012-12-07 | 2013-03-27 | 上海相宜本草化妆品股份有限公司 | Method for preparing mung bean sprout extracting solution and application of mung bean sprout extracting solution |
| CN106265430A (en) * | 2016-08-22 | 2017-01-04 | 彭之玉 | Semen phaseoli radiati ferment licorice peace skin frost and preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 张晓黎等: "乳酸菌发酵绿豆芽饮料工艺参数优化", 《农业科技与装备》 * |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108703924A (en) * | 2018-07-12 | 2018-10-26 | 佛山市汇汾化妆品科技有限公司 | A kind of plant Shu Min Essences and preparation method thereof |
| CN108785429B (en) * | 2018-07-31 | 2021-07-30 | 王世东 | A topical Chinese medicinal composition with soothing and repairing effects, and its preparation method |
| CN108785429A (en) * | 2018-07-31 | 2018-11-13 | 王世东 | A kind of external medicine composition and preparation method thereof repaired that has effects that releive |
| CN109481391A (en) * | 2018-12-04 | 2019-03-19 | 福建植护网络科技有限公司 | A kind of Baby Care cream and preparation method thereof compounding silver ion and green phenol song |
| CN109806175A (en) * | 2019-02-20 | 2019-05-28 | 高一铷 | A kind of addition moisturizing is releived gel combination |
| CN112516018A (en) * | 2019-09-18 | 2021-03-19 | 广州悦荟化妆品有限公司 | Selenium-rich mung bean fermentation liquor with whitening, anti-aging, moisturizing and toxin-expelling effects and mask composition thereof |
| CN111991316A (en) * | 2020-09-15 | 2020-11-27 | 广州莱约生物科技有限公司 | Anti-allergy repairing composition and preparation method and application thereof |
| CN111991316B (en) * | 2020-09-15 | 2023-06-30 | 广州莱约生物科技有限公司 | Anti-allergy repair composition, preparation method and application thereof |
| CN115006310A (en) * | 2022-07-05 | 2022-09-06 | 菏泽市亿鑫生物科技有限公司 | Mung bean sprout fermentation product, skin external preparation containing mung bean sprout fermentation product, and preparation method and application of mung bean sprout fermentation product |
| CN115006310B (en) * | 2022-07-05 | 2023-10-24 | 菏泽市亿鑫生物科技有限公司 | Mung bean sprout fermentation product, external skin preparation containing mung bean sprout fermentation product, and preparation method and application of external skin preparation |
| CN115040449A (en) * | 2022-07-08 | 2022-09-13 | 广州悦荟化妆品有限公司 | Mung bean fermentation liquor with anti-inflammatory, relieving and repairing effects, mixed fermentation liquor, and preparation method and application thereof |
| CN115089529A (en) * | 2022-08-03 | 2022-09-23 | 肽源（广州）生物科技有限公司 | Mung bean hull fermentation product with acne removing effect and preparation method and application thereof |
| CN115089529B (en) * | 2022-08-03 | 2023-07-25 | 肽源（广州）生物科技有限公司 | Mung bean hull fermentation product with acne removing effect, and preparation method and application thereof |
| CN115836997A (en) * | 2022-11-02 | 2023-03-24 | 菏泽市亿鑫生物科技有限公司 | Mung bean seed fermented product, skin external preparation containing mung bean seed fermented product, and preparation method and application of mung bean seed fermented product |
| CN117757848A (en) * | 2023-12-29 | 2024-03-26 | 山东福瑞达生物科技有限公司 | A kind of lactic acid bacteria fermentation product and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108186430A (en) | Sebum film, the mung bean zymotic fluid for soothing the skin allergy and preparation method thereof are damaged with repairing | |
| CN110200884B (en) | Composition with oil control and repair effects, preparation method thereof and application thereof in cosmetics | |
| CN111494282B (en) | Red-removing anti-inflammatory oil-controlling acne-removing composition, application thereof and acne-removing balancing essence containing composition | |
| CN105411960A (en) | Mask with acne inhibition effect and preparation method thereof | |
| CN117414312B (en) | Preparation method and application of hydrolyzed sclerotium rolfsii with repairing and relieving effects | |
| CN115317417A (en) | Composition containing guaiacum extract for repairing acne sensitive muscle and skin care product thereof | |
| CN108815072A (en) | A kind of plant composition fermentation magma and its preparation method and application | |
| CN115414308B (en) | Acne-removing composition, preparation method and application | |
| CN113057930A (en) | A kind of anti-mite face cream containing rosemary and preparation method thereof | |
| CN108354880A (en) | A kind of baby child's skin care compositions and methods | |
| CN116162552B (en) | Preparation method and application of extremely red aspergillus sorghum fermentation product | |
| CN111588815B (en) | External acne-removing traditional Chinese medicine composition, application thereof and acne-removing balancing essence containing composition | |
| CN115414299A (en) | A soothing and repairing freeze-dried spray | |
| CN111419733A (en) | Ceramide polypeptide compound Anmyo honey | |
| CN109381390A (en) | A kind of sodium hyaluronate skin lightening Face-protecting mask and preparation method thereof | |
| CN103877003A (en) | Nano-ferment skin-tendering and anti-aging lotion | |
| CN106727012A (en) | A kind of acne-eliminating cosmetic composition | |
| CN114788795A (en) | Acne-removing composition and preparation method and application thereof | |
| CN113244140A (en) | Composition for regulating skin micro-ecological balance and application and preparation method thereof | |
| Song et al. | Lactobacillus plantarum fermented Laminaria japonica alleviates UVB-induced epidermal photoinflammation via the Keap-1/Nrf2 pathway | |
| CN115054566B (en) | Scalp aging resisting composition and preparation method thereof | |
| CN110302132A (en) | A kind of reparation spray and preparation method thereof of the filtrate of secretion containing snail | |
| CN108721202A (en) | A kind of roselle stem cell freeze-dried powder eye cream and preparation method thereof | |
| TWI648054B (en) | Hydrolysate of water extract of Asparagus officinalis, preparation method and use thereof | |
| CN116492252B (en) | A repairing and anti-aging facial cream containing jasmine flower extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180622 |
|
| RJ01 | Rejection of invention patent application after publication |