CN108148777A - A kind of bacillus subtilis, complex micro organism fungicide, organic substrate and its preparation and application - Google Patents

Abstract

The invention belongs to the field of organic substrates, and in particular relates to a bacillus subtilis, a composite microbial agent, an organic substrate and its preparation and application. The Bacillus subtilis BS‑W1 of the present invention has a preservation number of CCTCC M 2017589. The bacillus subtilis can be prepared into a composite microbial bacterial agent, which can be composted at high temperature and then prepared into an organic matrix. Since the Bacillus subtilis strain has strong tolerance under 70°C fermentation conditions, and can secrete a variety of hydrolytic enzymes to efficiently degrade macromolecular organic matter such as protein, starch, and cellulose in raw materials, the compost center temperature can Rise to 70°C. Therefore, compared with the conventional composting technology, the composting fermentation temperature is increased, thereby significantly increasing the organic matter and total nutrient content, and can more effectively kill pathogenic bacteria, parasite eggs, weed seeds, etc.

Description

A kind of bacillus subtilis, composite microbial bacterial agent, organic matrix and its preparation and application

technical field

The invention belongs to the field of organic substrates, and in particular relates to a bacillus subtilis, a composite microbial agent, an organic substrate and its preparation and application.

Background technique

Organic substrate cultivation refers to the use of organic matter such as livestock and poultry manure, crop straw, mushroom residue, peat, sawdust, etc., after high-temperature fermentation treatment, mixed in a certain proportion to form a relatively stable and buffering full-nutrient cultivation substrate raw material. The cultivation substrate made entirely of organic matter has poor air permeability and water retention. Therefore, in order to improve the physical and chemical properties of the cultivation substrate, vermiculite, perlite, river sand, cinder and other inorganic substances can be mixed with it in a certain proportion to form an organic-inorganic substrate. Model cultivation substrate.

At present, the soilless cultivation technology using cultivation substrate is the most effective, economical and thorough way to overcome the obstacles of continuous cropping in greenhouses. Therefore, this technology has also been vigorously developed in some advanced agricultural countries abroad. In 1994, more than 40% of the 4,000 hectares of greenhouse crops in the Netherlands used substrate cultivation technology, and 7% of greenhouse vegetables in Israel used soilless cultivation, while the scale of soilless cultivation in my country is relatively backward. An important factor restricting the development of soilless cultivation in my country is the high cost of substrate. In order to solve this problem, the use of agricultural and animal husbandry wastes as raw materials to prepare organic substrates is a major development direction. The agricultural and animal husbandry wastes that can be used as organic matrix raw materials mainly include: livestock and poultry breeding waste, crop straw, agricultural product processing waste, wood chips, rice husk, edible fungus matrix waste, etc. Using these agricultural and animal husbandry wastes as raw materials to produce organic substrates, on the one hand, can greatly reduce the production cost of cultivation substrates, on the other hand, it also makes full use of organic waste resources and reduces environmental pollution.

High-temperature aerobic composting technology is a key technology for producing organic substrates from agricultural and animal husbandry organic waste, and it is also an effective way to realize its harmless, reduced, and resourceful treatment. High-temperature aerobic composting is the use of aerobic bacteria for high-temperature solid fermentation. The composting temperature is maintained above 50°C for at least 5 days. The high temperature kills pathogenic bacteria, parasite eggs, and weed seeds, so that organic matter is degraded and finally converted into stable humus. the process of. However, there are often two problems in the conventional high-temperature aerobic composting technology: 1) although the raw materials are rich in organic matter and some nutrients, these organic matter are all refractory macromolecular organic matter such as cellulose and protein, due to insufficient available nutrients, C/ If the N ratio is too high, it is difficult for compost microorganisms to obtain nutrients suitable for their rapid growth in a short period of time (24 or 48 hours), and if the added compost microbial strains cannot grow rapidly within a short period of time (5-7 days), they will It will lead to the failure of the bacteria, which is the phenomenon that the effect of starting composting with composting bacteria is not obvious. 2) Due to the failure of microorganisms to grow in large quantities in the early stage, they cannot effectively decompose macromolecular organic matter, that is, the compost temperature cannot rise rapidly to 60°C within 48 hours, resulting in the inability of complete fermentation of raw materials, and the content of organic matter in compost products is too high. lead to seedling burn.

Based on the above technical problems, it is the key to solve the problem to develop an organic matrix raw material formula that can make the composting microorganisms grow rapidly and start the composting process quickly.

Contents of the invention

In order to overcome the problems existing in the prior art, the object of the present invention is to provide a bacillus subtilis, a composite microbial bacterial agent, an organic substrate and the preparation and application thereof. The invention aims to solve the problem that the composting effect of microorganisms is not obvious in the existing high-temperature aerobic composting technology.

In order to achieve the above object and other related objects, the present invention adopts the following technical solutions:

The first aspect of the present invention provides a Bacillus subtilis BS-W1, the preservation number of which is: CCTCC M 2017589.

The present invention obtains a strain of Bacillus subtilis BS-W1 from livestock and poultry feces high-temperature composting raw materials. The strain has been preserved in the China Center for Type Culture Collection on October 19, 2017, and the preservation number is CCTCC M 2017589.

The bacillus subtilis isolated in the invention can withstand high temperature composting environment (70°C). And it can secrete a variety of enzymes to efficiently degrade compost materials such as manure and straw.

After morphological identification and 16S rRNA, ITS-2 and rbcL amplified sequence developmental tree analysis, it was determined that the strain was Bacillus subtilis (according to the international naming rules: genus name + species name + strain name to name the strain, genus name, The species name and strain name are respectively Bacillus, subtilis and BS-W1), named Bacillus subtilis BS-W1, and the preservation number is: CCTCC M2017589. The strain has the following advantages: it has strong tolerance to high temperature environment (70°C). It can secrete a variety of enzymes to efficiently degrade compost materials such as manure and straw.

The second aspect of the present invention provides the use of the aforementioned Bacillus subtilis BS-W1 for high-temperature aerobic composting.

The third aspect of the present invention provides a composite microbial bacterial agent for high-temperature aerobic composting, including Bacillus subtilis, Aspergillus niger, Bacillus amyloliquefaciens, Candida tropicalis, Trichoderma reesei, Streptomyces grisea, Phanerochaete sporogenes, Pseudomonas fluorescens, Lactobacillus brevis, Bacillus longum.

The preservation number of the Bacillus subtilis is CCTCC M 2017589.

The Latin name of the Aspergillus niger is Aspergillus niger. Available from Culture Collections.

The Latin name of the Bacillus amyloliquefaciens is Bacillus amyloliquefaciens. Available from Culture Collections.

The Latin name of the Candida tropicalis is Candida tropicalis. Available from Culture Collections.

The Latin name of Trichoderma reesei is Trichoderma reesei. Available from Culture Collections.

The Latin name of said Streptomyces griseorubens is Streptomyces griseorubens. Available from Culture Collections.

The Latin name of the Phanerochaete chrysosporium is Phanerochaete chrysosporium. Available from Culture Collections.

The Latin name of the Pseudomonas fluorescens is Psdeuomnoda fluorerncnet. Available from Culture Collections.

The Latin name of the Lactobacillus brevis is Lactobacillus breris. Available from Culture Collections.

The Latin name of the lysinibacillus macroides is Lysinibacillus macroides. Available from Culture Collections.

Further, in the composite microbial bacterial agent, the range of parts by weight between the components is:

1-5 parts by weight of Bacillus subtilis, 1-5 parts by weight of Aspergillus niger, 1-5 parts by weight of Bacillus amyloliquefaciens, 1-5 parts by weight of Candida tropicalis, 1-3 parts by weight of Trichoderma reesei, gray slightly red 1-3 parts by weight of Streptomyces, 1-3 parts by weight of Phanerochaete chrysosporium, 1-3 parts by weight of Pseudomonas fluorescens, 1-3 parts by weight of Lactobacillus brevis, 1-3 parts by weight of Bacillus longum. share.

The fourth aspect of the present invention provides a method for preparing the above-mentioned composite microbial agent for high-temperature aerobic composting, which includes the steps of uniformly mixing the components.

The fifth aspect of the present invention provides the use of the aforementioned composite microbial agent for high-temperature aerobic composting in the preparation of organic substrates.

According to the sixth aspect of the present invention, an organic substrate is provided, and the organic substrate includes the aforementioned composite microbial agent.

Further, an organic substrate is provided, comprising the following components in parts by weight:

Further, it also includes an appropriate amount of culture wastewater RO concentrate.

The waste fermented bedding material refers to fermented bedding material for livestock and poultry breeding. It is mainly fermented mattress material for pig and cattle raising, and its main components are sawdust, rice husk, straw, etc.

The solid manure refers to the manure of livestock and poultry, mainly the manure of pigs, cows, chickens and the like.

The pomace refers to the remaining pulp and pericarp after fruit juice is squeezed and processed. It can be selected from any one or more of apple pomace, pear pomace, citrus pomace, pomelo pomace, peach pomace and the like. Pomace is rich in quick-acting carbon sources such as glucose, fructose, sucrose, and citric acid. Compared with long-acting carbon sources such as cellulose, it can make bacteria grow rapidly in a short time and start the composting process quickly.

The biochar refers to biochar prepared by carbonizing livestock and poultry feces under high temperature and anoxic conditions.

The hulled bran can be obtained through commercial channels.

The peat can be obtained through commercial channels.

The perlite is commercially available.

The RO concentrated liquid of breeding wastewater refers to the RO concentrated liquid obtained by treating livestock and poultry breeding wastewater by a double-membrane method (ultrafiltration membrane, RO membrane). After the aquaculture wastewater is treated by the double-membrane method, the RO permeate can reach the discharge standard. The remaining RO concentrate is rich in small molecular organics such as ammonia nitrogen and cannot be discharged directly. It is often returned to the original solution for further treatment. However, the ammonia nitrogen in the RO concentrate is an available nitrogen source for microbial growth, which can also make the bacteria grow rapidly in a short time and quickly start the composting process.

In a seventh aspect of the present invention, there is provided a method for preparing the aforementioned organic matrix, comprising the following steps:

(1) After drying the discarded fermented bedding material, mix the feces and bedding material therein evenly, pulverize, and set aside;

(2) pulverize the dried waste pomace for subsequent use;

(3) Dry the solid feces, pulverize them, and carry out carbonization treatment under anoxic conditions at 500-600°C to obtain biochar;

(4) Mix the discarded fermented bedding material after step (1), solid feces, the pomace after step (2), the biochar obtained in step (3), and rice bran according to a certain ratio;

(5) Spray the culture wastewater RO concentrate on the material obtained in step (4), and mix evenly;

(6) adding composite microbial bacterial agent to the mixed material obtained in step (5) according to the proportioning ratio;

(7) Carry out fermenting and decomposing, and fermenting and decomposing finishes, and dries;

(8) Mix the fermented and decomposed material with peat and perlite according to a certain ratio, control the water content to be less than 30wt%, sieve and pack, and obtain the organic matrix.

Preferably, in step (1), pulverize to a particle size of 1-2 mm.

Preferably, in step (2), pulverize to a particle size of 1-2 mm.

Preferably, in step (3), pulverize to a particle size of 1-2 mm.

Preferably, in step (3), the treatment time is 1-3h. It is further preferred to treat for 2h.

Preferably, in step (5), the moisture content of the mixed material is controlled within the range of 55-70 wt%, and the pH value is 6-9.

Preferably, in step (6), the composite microbial bacterial agent is added by stirring or spraying.

Preferably, in step (8), conventional fermenting bin composting, bar stacking composting or static composting methods are used to ferment and decompose, with a stacking height of 1.5-2 meters and a width of 1.5-2 meters. When the material temperature rises to 55-60 Turn the compost at ℃, turn it once every 2-5 days, control the fermentation temperature to 55-70℃, and the fermentation time to 10-40 days. , the fermentation is complete.

The eighth aspect of the present invention provides the use of the aforementioned organic substrate for planting crops.

Compared with the prior art, the present invention has the following beneficial effects:

(1) The present invention uses fruit pomace and culture wastewater RO concentrate in the matrix raw material formula, provides quick-acting carbon source and nitrogen source for composting microorganism, makes it grow in a large amount in a short time and starts composting process rapidly, therefore more conventional The advanced high-temperature aerobic composting technology shortens the composting time. The two raw materials are agricultural product processing waste and livestock and poultry breeding waste respectively, which will not increase the production cost of the organic matrix, and have obvious composting and fermentation effects.

(2) In the high-temperature aerobic composting technology of the present invention, a self-selected high-temperature composting fermentation strain—Bacillus subtilis is used, which has strong tolerance under 70°C fermentation conditions and can secrete various hydrolytic enzymes Efficiently degrade macromolecular organic matter such as protein and cellulose in raw materials, but the composting temperature rises to 70°C. Therefore, compared with the conventional high-temperature aerobic composting technology, the compost fermentation temperature is increased, thereby more effectively killing pathogenic bacteria, parasite eggs, weed seeds, etc.

(3) A variety of agricultural and animal husbandry wastes are used as raw materials for the preparation of the organic matrix of the present invention, such as 1) waste fermentation mattress material: through high-temperature composting and fermentation, the undegraded solid feces are fully degraded, and can effectively kill the waste in the feces. pathogenic bacteria, and the wood chips and rice husks in the bedding are used as components in the organic matrix formula, which can increase water retention and porosity; , within a few hours, it will be spoiled and acidified, breeding insects and flies. Applying pomace to the high-temperature composting fermentation of organic substrates can make full use of the available carbon source and inhibit the growth and reproduction of spoilage bacteria; 3) Biochar : The biochar prepared by livestock and poultry manure is added to the organic matrix, which has the functions of loosening and ventilating, retaining water and fertilizer, cultivating beneficial microorganisms, and passivating heavy metals. Using these agricultural and animal husbandry wastes to produce organic substrates not only gives full play to the advantages of various components, but also truly realizes the harmless, reduced, and resourceful treatment of organic wastes.

The strain preservation information of the present invention is as follows:

Strain name: Bacillus subtilis BS-W1;

The deposit number is: CCTCC NO: M 2017589;

Deposit date: October 19, 2017;

Name of depository unit: China Center for Type Culture Collection;

Abbreviation of depository unit: CCTCC;

Address of depository unit: Wuhan University, Wuhan, China.

Detailed ways

Before further describing the specific embodiments of the present invention, it should be understood that the protection scope of the present invention is not limited to the following specific specific embodiments; it should also be understood that the terms used in the examples of the present invention are to describe specific specific embodiments, It is not intended to limit the protection scope of the present invention. The test methods for which specific conditions are not indicated in the following examples are usually in accordance with conventional conditions, or in accordance with the conditions suggested by each manufacturer.

When the examples give numerical ranges, it should be understood that, unless otherwise stated in the present invention, the two endpoints of each numerical range and any value between the two endpoints can be selected. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition to the specific methods, equipment, and materials used in the embodiments, according to those skilled in the art's grasp of the prior art and the description of the present invention, the methods, equipment, and materials described in the embodiments of the present invention can also be used Any methods, apparatus and materials of the prior art similar or equivalent to the practice of the present invention.

Example 1 Acquisition, Identification and Preservation of Bacillus subtilis

The present invention obtains a strain of Bacillus subtilis BS-W1 from livestock and poultry feces high-temperature composting raw materials. The strain has been preserved in the China Center for Type Culture Collection on October 19, 2017, and the preservation number is CCTCC M 2017589.

The bacillus subtilis isolated in the invention can withstand high temperature composting environment (70°C). And it can secrete a variety of enzymes to efficiently degrade compost materials such as manure and straw.

After morphological identification and 16S rRNA, ITS-2 and rbcL amplified sequence developmental tree analysis, it was determined that the strain was Bacillus subtilis (according to the international naming rules: genus name + species name + strain name to name the strain, genus name, The species name and strain name are respectively Bacillus, subtilis and BS-W1), named Bacillus subtilis BS-W1, and the preservation number is: CCTCC M2017589. The strain has the following advantages: it has strong tolerance to high temperature environment (70°C). It can secrete a variety of enzymes to efficiently degrade compost materials such as manure and straw.

Example 2 Composite microbial bacterial agent for high-temperature aerobic composting

The Bacillus subtilis BS-W1 of the invention can be used for high-temperature aerobic composting. Generally, it is formulated with other microorganisms into a composite microbial agent for high-temperature aerobic composting.

The range of parts by weight between the components in the composite microbial agent 1 is: 1 part by weight of Bacillus subtilis, 5 parts by weight of Aspergillus niger, 1 part by weight of Bacillus amyloliquefaciens, 5 parts by weight of Candida tropicalis, 1 part by weight of Trichoderma, 3 parts by weight of Streptomyces grisea, 1 part by weight of Phanerochaete chrysosporium, 3 parts by weight of Pseudomonas fluorescens, 1 part by weight of Lactobacillus brevis, and 3 parts by weight of Bacillus longum. .

The range of parts by weight between the components in the composite microbial agent 2 is: 5 parts by weight of Bacillus subtilis, 1 part by weight of Aspergillus niger, 5 parts by weight of Bacillus amyloliquefaciens, 1 part by weight of Candida tropicalis, 3 parts by weight of Trichoderma, 1 part by weight of Streptomyces grisea, 3 parts by weight of Phanerochaete chrysosporium, 1 part by weight of Pseudomonas fluorescens, 3 parts by weight of Lactobacillus brevis, and 1 part by weight of Bacillus longum. .

The range of parts by weight between the components in the composite microbial agent 3 is: 2 parts by weight of Bacillus subtilis, 3 parts by weight of Aspergillus niger, 4 parts by weight of Bacillus amyloliquefaciens, 3 parts by weight of Candida tropicalis, 2 parts by weight of Trichoderma, 2 parts by weight of Streptomyces grisea, 2 parts by weight of Phanerochaete chrysosporium, 2.5 parts by weight of Pseudomonas fluorescens, 2 parts by weight of Lactobacillus brevis, 2.5 parts by weight of Bacillus longum. .

The formula of the organic matrix raw material is: 30 parts by weight of waste cattle fermented bedding material, 30 parts by weight of solid cow feces, 20 parts by weight of apple pomace, 8 parts by weight of biochar, 6 parts by weight of rice chaff, 3 parts by weight of peat, and 3 parts by weight of perlite. parts by weight.

Organic substrate 1: 30 parts by weight of waste cattle fermented bedding material, 30 parts by weight of solid cow manure, 20 parts by weight of apple pomace, 8 parts by weight of biochar, 6 parts by weight of chaff, 3 parts by weight of peat, and 3 parts by weight of perlite , without any compound microbial agent, composted and decomposed directly;

Organic substrate 2: 30 parts by weight of waste cattle fermented bedding material, 30 parts by weight of bovine solid manure, 20 parts by weight of apple pomace, 8 parts by weight of biochar, 6 parts by weight of chaff, 3 parts by weight of peat, and 3 parts by weight of perlite , add compound microbial bacterial agent 1, and the compost becomes decomposed.

Organic substrate 3: 30 parts by weight of waste fermented bedding material for raising cattle, 30 parts by weight of solid cow manure, 20 parts by weight of apple pomace, 8 parts by weight of biochar, 6 parts by weight of rice chaff, 3 parts by weight of peat, and 3 parts by weight of perlite , add compound microbial bacterial agent 2, and the compost is decomposed.

Organic matrix 4: 30 parts by weight of waste cattle fermented bedding material, 30 parts by weight of solid cow manure, 20 parts by weight of apple pomace, 8 parts by weight of biochar, 6 parts by weight of chaff, 3 parts by weight of peat, and 3 parts by weight of perlite , add compound microbial agent 3, and the compost becomes decomposed.

Table 1

As can be seen from the data in Table 1 above, the use of the compound microbial agent of the present invention to prepare the organic matrix can achieve a maximum center temperature of 70°C during the composting process, which is significantly higher than the compost temperature of 55°C without the application of the agent, which can greatly kill Eliminate harmful microorganisms and insect eggs in the organic substrate; the organic substrate prepared by it has significantly improved organic matter and total nutrient content, and can increase the germination index of seeds.

Example 3 Preparation of organic matrix using cattle waste and apple pomace

Organic matrix formula in the present embodiment:

The parts by weight of each raw material are: 30 parts by weight of waste cattle breeding fermented bedding material, 30 parts by weight of cow solid manure, 20 parts by weight of apple pomace, 8 parts by weight of biochar, 6 parts by weight of rice bran, 3 parts by weight of peat, pearl 3 parts by weight of rock, and 1 part by weight of composite microbial bacterial agent is added to ferment. The composition and parts by weight of the composite microbial bacterial agent in the present embodiment are: 3 parts of Bacillus subtilis, 5 parts of Aspergillus niger, 3 parts of Bacillus amyloliquefaciens, 3 parts of Candida tropicalis, 3 parts of Trichoderma reesei, 1 part of Streptomyces rubrum, 3 parts of Phanerochaete chrysosporium, 1 part of Pseudomonas fluorescens, 1 part of Lactobacillus brevis, and 1 part of Bacillus longum.

A method for preparing an organic substrate, comprising the steps of:

(1) Collect the discarded cattle fermented bedding materials, dry them, mix the feces and bedding materials evenly, and crush them until the particle size is 1-2mm;

(2) collect apple pomace, dry, be pulverized to particle diameter and be 1-2mm;

(3) Dry the bovine solid feces after solid-liquid separation, crush them to 1-2 mm, and treat them under anoxic conditions at 500-600° C. for 2 hours to obtain biochar;

(4) Mix the discarded cattle fermented bedding material, cattle solid manure, apple pomace, biochar, and rice bran according to the formula ratio;

(5) Spray the RO concentrate of cattle raising wastewater on the material and mix evenly, control the moisture content of the mixed material at 60%, and adjust the pH value to 7;

(6) Sprinkle the compound microbial bacterial agent evenly on the material, and use the strip-stack composting method to ferment and decompose. The stacking height is 1.5 meters and the width is 2 meters. Turn the pile once a day, control the fermentation temperature to 60-70°C, and the fermentation time to 15 days. When the fermentation temperature no longer rises and the volume of the compost collapses by 1/3 to 1/2, the fermentation is complete and dried in the air;

(7) Mix the fermented and decomposed material with peat and perlite according to the formula ratio, control the moisture content to be less than 30wt%, sieve and pack, and obtain the organic matrix.

Example 4 Preparation of organic matrix using pig waste and pear residue

Organic matrix formula in the present embodiment:

The parts by weight of each raw material are: 40 parts by weight of waste pig fermented bedding material, 20 parts by weight of pig solid waste, 15 parts of pear dregs, 10 parts of biochar, 5 parts of rice chaff, 1 part of peat, and 5 parts of perlite. fermentation. The composition and parts by weight of the composite microbial bacterial agent in the present embodiment are: 5 parts of Bacillus subtilis, 3 parts of Aspergillus niger, 5 parts of Bacillus amyloliquefaciens, 1 part of Candida tropicalis, 2 parts of Trichoderma reesei, 1 part of Streptomyces rubrum, 1 part of Phanerochaete chrysosporium, 1 part of Pseudomonas fluorescens, 1 part of Lactobacillus brevis, and 1 part of Bacillus longum.

A method for preparing a base matrix, comprising the steps of:

(1) Collect the discarded pig fermented bedding materials, dry them, mix the feces and bedding materials evenly, and crush them until the particle size is 1-2mm;

(2) collect pear residue, dry, be pulverized to particle diameter and be 1-2mm;

(3) Dry the pig solid feces after solid-liquid separation, crush them to 1-2mm, and treat them under anoxic conditions at 500-600°C for 2 hours to obtain biochar;

(4) Mix the discarded pig fermented bedding material, pig solid manure, pear dregs, biochar, and rice bran evenly according to the formula ratio;

(5) Spray pig raising wastewater RO concentrate on the material and mix evenly, control the moisture content of the mixed material at 55%, and adjust the pH value to 9;

(6) Sprinkle the compound microbial agent evenly on the material, and use the strip-stack composting method to ferment and decompose. The stacking height is 2 meters and the width is 1.5 meters. Turn the pile once a day, control the fermentation temperature to 55-70°C, and the fermentation time to 10 days. When the fermentation temperature no longer rises and the volume of the compost collapses by 1/3 to 1/2, the fermentation is complete and dried in the air;

(7) Mix the fermented and decomposed material with peat and perlite according to the formula ratio, control the moisture content to be less than 30wt%, sieve and pack, and obtain the organic matrix.

Example 5 Preparation of organic matrix using waste from cattle and pigs and citrus pomace

Organic matrix formula in the present embodiment:

The parts by weight of each raw material are as follows: 50 parts by weight of waste cattle fermented bedding material, 10 parts by weight of pig solid manure, 10 parts by weight of citrus residue, 5 parts by weight of biochar, 10 parts by weight of rice bran, 5 parts by weight of peat, and 1 part by weight of perlite. parts by weight, add 2 parts by weight of composite microbial bacterial agent to ferment. The composition and parts by weight of the composite microbial bacterial agent in the present embodiment are: 1 part of Bacillus subtilis, 1 part of Aspergillus niger, 1 part of Bacillus amyloliquefaciens, 5 parts of Candida tropicalis, 1 part of Trichoderma reesei, 3 parts of Streptomyces rubrum, 3 parts of Phanerochaete chrysosporium, 3 parts of Pseudomonas fluorescens, 3 parts of Lactobacillus brevis, and 3 parts of Bacillus longum.

A method for preparing an organic substrate, comprising the steps of:

(1) Collect the discarded fermented bedding materials for cattle breeding, dry them, mix the feces and bedding materials evenly, and pulverize them until the particle size is 1-2mm;

(2) collect citrus pomace, dry, and pulverize to a particle size of 1-2mm;

(3) Dry the pig solid feces after solid-liquid separation, crush them to 1-2mm, and treat them under anoxic conditions at 500-600°C for 2 hours to obtain biochar;

(4) Mix the waste fermented bedding material for cattle raising, pig solid manure, citrus residue, biochar, and rice bran according to the formula ratio;

(5) Spray the RO concentrate of pig raising wastewater on the material and mix evenly, control the moisture content of the mixed material at 70%, and adjust the pH value to 6;

(6) Sprinkle the compound microbial bacterial agent evenly on the material, and use the strip-stack composting method to ferment and decompose. The stacking height is 1.5 meters and the width is 2 meters. Turn the compost once a day, control the fermentation temperature to 65-70°C, and the fermentation time to 40 days. When the fermentation temperature no longer rises and the volume of the compost collapses by 1/3 to 1/2, the fermentation is complete and dried in the air;

(7) Mix the fermented and decomposed material with peat and perlite according to the formula ratio, control the moisture content to be less than 30wt%, sieve and pack, and obtain the organic matrix.

Embodiment 6 Comparison of different organic matrix effects

In the same greenhouse, strawberries were planted with the organic substrates prepared in Examples 3-5 respectively, the control group 1 was soil, and the control group 2 was the composition ratio of Example 1 (the weight portion of each composition raw material is: abandoned cattle breeding Fermented mattress material 30, solid cow manure 30, apple pomace 20, biochar 8, rice chaff 6, peat 3, perlite 3), but do not add the compound microbial bacterial agent of this patent, the organic matrix obtained by natural composting.

Cultivate the same variety, and the daily management measures are the same. The application effect is as follows:

As can be seen from the above data, after using the organic matrix prepared from livestock and poultry waste and pomace in the present invention, the strawberry production can be increased by 12.6-20.4%, the soluble solid content increases compared with the contrast, and the soluble sugar content also increases. Titrated acid decreased slightly. The yield and quality of strawberries grown on organic substrates have improved.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any form and in essence. Several improvements and supplements can be made, and these improvements and supplements should also be regarded as the protection scope of the present invention. Those who are familiar with this profession, without departing from the spirit and scope of the present invention, when they can use the technical content disclosed above to make some changes, modifications and equivalent changes of evolution, are all included in the present invention. Equivalent embodiments; at the same time, all changes, modifications and evolutions of any equivalent changes made to the above-mentioned embodiments according to the substantive technology of the present invention still belong to the scope of the technical solution of the present invention.

Claims (12)

1. a kind of bacillus subtilis, preserving number is CCTCC M 2017589.

2. bacillus subtilis as described in claim 1 is used for the purposes of high temperature aerobic composting.

3. a kind of complex micro organism fungicide, including bacillus subtilis, aspergillus niger, bacillus amyloliquefaciens, candida tropicalis, Trichoderma reesei, ash slightly red streptomyces, Phanerochaete chrysosporium, Pseudomonas fluorescens, Lactobacillus brevis, long lysine bacillus.

4. complex micro organism fungicide according to claim 3, which is characterized in that the preserving number of the bacillus subtilis is CCTCC M 2017589。

5. complex micro organism fungicide according to claim 3, which is characterized in that in the complex micro organism fungicide, each group It is into the parts by weight ratio range between ingredient：1~5 parts by weight of bacillus subtilis, 1~5 parts by weight of aspergillus niger, solution starch 1~5 parts by weight of bacillus, 1~5 parts by weight of candida tropicalis, 1~3 parts by weight of trichoderma reesei, ash slightly red streptomyces 1~3 Parts by weight, 1~3 parts by weight of Phanerochaete chrysosporium, 1~3 parts by weight of Pseudomonas fluorescens, 1~3 parts by weight of Lactobacillus brevis, length 1~3 parts by weight of lysine bacillus.

6. the preparation method of complex micro organism fungicide as described in claim any one of 3-5, including step：Each constituent is equal Even mixing.

7. purposes of the complex micro organism fungicide in organic substrate is prepared as described in claim any one of 3-5.

8. a kind of organic substrate includes the constituent of following parts by weight：

9. organic substrate according to claim 8, which is characterized in that it is appropriate to further include breeding wastewater RO concentrates.

10. the preparation method of organic substrate as claimed in claim 8 or 9, which is characterized in that include the following steps：

(1) after discarded fermenting bed padding is dried, excrement therein and bedding and padding are uniformly mixed, crushed, it is spare；

(2) the discarded pomace after drying crushes, spare；

(3) solid manure is dried, crushes, charing process is carried out under 500-600 DEG C of anoxia condition, obtains charcoal；

(4) pomace after discarded fermenting bed padding, solid manure, step (2) crushing after step (1) is crushed, step (3) institute Obtain charcoal, rice chaff is uniformly mixed according to a certain percentage；

(5) breeding wastewater RO concentrates are sprayed on step (4) resulting material, be uniformly mixed；

(6) by proportioning complex micro organism fungicide is added in into mixed material obtained by step (5)；

(7) fermentation maturity is carried out, fermentation maturity terminates, dries；

(8) the good material of fermentation maturity and turf, perlite are mixed according to a certain percentage, control moisture is less than 30wt%, sub-sieve, packaging are to get organic substrate.

11. preparation method as claimed in claim 10 further includes any one of following characteristics or multinomial：A) in step (1), Grain size is crushed to as 1-2mm；B) in step (2), grain size is crushed to as 1-2mm；C) in step (3), grain size is crushed to as 1- 2mm；D) processing time is 1-3h in step (3)；E) in step (5), the moisture of material is in 55-70wt% after control mixing In the range of, pH value 6-9；F) in step (6), complex micro organism fungicide is added in a manner of stirring or spray；G) in step (8), Fermentation maturity is carried out using conventional fermentation cabin compost, bar buttress formula compost or static compost method, 1.5-2 meters of stacking height is wide 1.5-2 meters of degree, starts turning when temperature of charge rises to 55-60 DEG C, primary per turning in 2-5 days, and control fermentation temperature is 55-70 DEG C, fermentation time is 10-40 days, and when fermentation temperature no longer increases, compost volume collapses 1/3~1/2, fermentation maturity terminates.

12. organic substrate as claimed in claim 8 or 9 is used for the purposes of proportion of crop planting.