CN108148761A - A kind of composition for improving Eimeria Tenella egg capsule yield and preparation method thereof - Google Patents
A kind of composition for improving Eimeria Tenella egg capsule yield and preparation method thereof Download PDFInfo
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- CN108148761A CN108148761A CN201711493913.1A CN201711493913A CN108148761A CN 108148761 A CN108148761 A CN 108148761A CN 201711493913 A CN201711493913 A CN 201711493913A CN 108148761 A CN108148761 A CN 108148761A
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- composition
- egg capsule
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- eimeria tenella
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- 239000002775 capsule Substances 0.000 title claims abstract description 57
- 239000000203 mixture Substances 0.000 title claims abstract description 49
- 241000223932 Eimeria tenella Species 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 241000218202 Coptis Species 0.000 claims abstract description 19
- 235000002991 Coptis groenlandica Nutrition 0.000 claims abstract description 19
- 241000241413 Propolis Species 0.000 claims abstract description 16
- 229940069949 propolis Drugs 0.000 claims abstract description 16
- VAOCPAMSLUNLGC-UHFFFAOYSA-N metronidazole Chemical compound CC1=NC=C([N+]([O-])=O)N1CCO VAOCPAMSLUNLGC-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229960000282 metronidazole Drugs 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000005030 aluminium foil Substances 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims 1
- 238000007789 sealing Methods 0.000 claims 1
- 241000287828 Gallus gallus Species 0.000 abstract description 16
- 229960005486 vaccine Drugs 0.000 abstract description 13
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 210000004347 intestinal mucosa Anatomy 0.000 abstract description 4
- 241001148471 unidentified anaerobic bacterium Species 0.000 abstract description 4
- 241000252983 Caecum Species 0.000 abstract description 3
- 210000004534 cecum Anatomy 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 239000013022 formulation composition Substances 0.000 abstract description 3
- 230000000968 intestinal effect Effects 0.000 abstract description 3
- 208000003495 Coccidiosis Diseases 0.000 abstract description 2
- 206010023076 Isosporiasis Diseases 0.000 abstract description 2
- 230000002757 inflammatory effect Effects 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 241000224483 Coccidia Species 0.000 description 5
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 238000011081 inoculation Methods 0.000 description 5
- 210000004681 ovum Anatomy 0.000 description 5
- 235000013599 spices Nutrition 0.000 description 5
- 239000000284 extract Substances 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- 210000003250 oocyst Anatomy 0.000 description 3
- 229960000074 biopharmaceutical Drugs 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000014837 parasitic helminthiasis infectious disease Diseases 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/10—Protozoa; Culture media therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/002—Protozoa antigens
- A61K39/012—Coccidia antigens
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of compositions for improving Eimeria Tenella egg capsule yield and preparation method thereof, and the composition component and its ratio of weight and number are：30 50 parts of Cortex Phellodendri, 0.5 5 parts of propolis, 20 60 parts of the coptis, 10 20 parts of metronidazole.Inventive formulation composition is scientific and reasonable, and simple for process, easy to use, cost is relatively low.The present composition can be by preventing intestinal inflammatory, inhibiting the breeding of anaerobic bacteria in caecum, histocyte is promoted to regenerate, accelerate the effects that intestinal mucosa is repaired and improve the yield of Eimeria Tenella egg capsule, it reduces egg capsule and expands numerous chicken quantity used, lower human and material resources, time cost, so as to greatly improve the production efficiency of vaccine to chicken coccidiosis.
Description
Technical field
The invention belongs to veterinary biologics field, especially a kind of combination for improving Eimeria Tenella egg capsule yield
Object and preparation method thereof.
Background technology
Chicken coccidiasis causes the economic damage of billions of members in China every year as a kind of common disease for endangering poultry husbandry
It loses.It is the main method for preventing global-worm illness using chemicals at present, but remains that all kinds of corrupt practices creep in, such as medicament residue, environment
Pollution etc..And coccidial vaccine has the advantage of its own as a kind of biological agent：Green high-efficient, pollution-free, noresidue etc..But its
Some resistances are also faced in actually promoting and applying, one of main cause is exactly of high cost.The production cost of coccidian oocyst
More than 80% coccidial vaccine totle drilling cost is accounted for, therefore the egg capsule yield height of single chicken determines the height of coccidial vaccine cost,
The egg capsule yield for how improving single chicken becomes main direction of studying for veterinary biologics practitioner.
Invention content
The purpose of the present invention is to provide a kind of composition for improving Eimeria Tenella egg capsule yield and its preparation sides
Method.Inventive formulation composition is scientific and reasonable, and simple for process, easy to use, cost is relatively low.The present composition is by preventing enteron aisle
Inflammation inhibits the breeding of anaerobic bacteria in caecum, promotes the effects that histocyte regeneration, quickening intestinal mucosa reparation tender to improve
The yield of eimeria tenella egg capsule reduces egg capsule and expands numerous chicken quantity used, lowers human and material resources, time cost, so as to big
The big production efficiency for improving vaccine to chicken coccidiosis.
It is another object of the present invention to a kind of composition for improving Eimeria Tenella egg capsule yield and its preparations
Method.
The present invention is achieved by the following technical solutions：
A kind of composition and preparation method thereof for improving Eimeria Tenella egg capsule yield, the component of the composition and its
Ratio of weight and number is：
20-50 parts of Cortex Phellodendri
0.1-5 parts of propolis
20-60 parts of the coptis
10-20 parts of metronidazole
Moreover, the composition component and its optimal ratio of weight and number are respectively：
35 parts of Cortex Phellodendri
0.2 part of propolis
50 parts of the coptis
14.8 parts of metronidazole.
A kind of the step of composition for improving Eimeria Tenella egg capsule yield and preparation method thereof, preparation method
For：
(1) Cortex Phellodendri is weighed respectively according to weight proportion, the coptis crushes and cross 60 mesh sieve, be put into mixing tank, mix
30 minutes spare.
(2) weigh propolis according to weight proportion, crush and sieve with 100 mesh sieve, be put into equipped with Cortex Phellodendri, the coptis mixing tank in it is standby
With.
(3) according to weight proportion weigh metronidazole be put into equipped with Cortex Phellodendri, the coptis, propolis mixing tank in mix 30 minutes.
It dispenses into aluminium foil bag, seals after mixing, you can obtain a kind of composition for improving Eimeria Tenella egg capsule yield.
The composition according to the present invention for improving Eimeria Tenella egg capsule yield is to be applied to by the following method
Coccidial vaccine production：10 days before and after the inoculation of Eimeria Tenella egg capsule vaccine strain, to chicken, group feeds combination of the present invention
Object per 100g the composition spice 200kg, is used in conjunction 10 days.
Advantages of the present invention and advantageous effect are：
1. the purpose of the present invention is to provide a kind of composition for improving Eimeria Tenella egg capsule yield and its preparations
Method.Inventive formulation composition is scientific and reasonable, and simple for process, easy to use, cost is relatively low.
2. the present invention mitigates destruction of the bacterium to intestinal mucosa, to coccidian oocyst using Cortex Phellodendri, coptis prevention intestinal inflammatory
A good intestinal environment is provided in the field planting of gut epithelium, so as to increase the field planting rate of egg capsule, improves tender Amy that ball
The yield of worm's ovum capsule
3. the present invention inhibits the breeding of anaerobic bacteria in caecum using metronidazole, prevent the breeding because of anaerobic bacteria from influencing egg capsule
Normal development so that improve Eimeria Tenella egg capsule yield.
4. the present invention using propolis come promote histocyte regeneration, accelerate intestinal mucosa reparation for the schizogamy of coccidia and
Gamogenesis provides enough epithelial cell and then come the yield that improves Eimeria Tenella egg capsule.
Specific embodiment
The present invention is described in further detail by following embodiment.It should be noted that：Following embodiments are illustrative, are not
It is limited, it is impossible to limit protection scope of the present invention with following embodiments.
Cortex Phellodendri, propolis, the coptis in component of the present invention, metronidazole can be commercially available on the market.
Embodiment 1
A kind of composition and preparation method thereof for improving Eimeria Tenella egg capsule yield, the component of the composition and its
Weight is respectively：Cortex Phellodendri 35kg, propolis 0.2kg, coptis 50kg, metronidazole 14.8kg.
A kind of the step of composition for improving Eimeria Tenella egg capsule yield and preparation method thereof, preparation method
For：
1) Cortex Phellodendri is weighed respectively according to weight proportion, the coptis crushes and cross 60 mesh sieve, be put into mixing tank, mixing 30
Minute is spare.
(2) weigh propolis according to weight proportion, crush and sieve with 100 mesh sieve, be put into equipped with Cortex Phellodendri, the coptis mixing tank in it is standby
With.
(3) according to weight proportion weigh metronidazole be put into equipped with Cortex Phellodendri, the coptis, propolis mixing tank in mix 30 minutes.
It dispenses into aluminium foil bag, seals after mixing, you can obtain a kind of composition for improving Eimeria Tenella egg capsule yield.
The composition according to the present invention for improving Eimeria Tenella egg capsule yield is to be applied to by the following method
Coccidial vaccine production：10 days before and after the inoculation of Eimeria Tenella egg capsule vaccine strain, to chicken, group feeds combination of the present invention
Object per 100g the composition spice 200kg, is used in conjunction 10 days.
Embodiment 2
A kind of composition and preparation method thereof for improving Eimeria Tenella egg capsule yield, the component of the composition and its
Weight is respectively：Cortex Phellodendri 30kg, propolis 0.1kg, coptis 40kg, metronidazole 10kg.
Preparation method and application are the same as embodiment 1.
Embodiment 3
A kind of composition and preparation method thereof for improving Eimeria Tenella egg capsule yield, the component of the composition and its
Weight is respectively：Cortex Phellodendri 45kg, propolis 0.4kg, coptis 35kg, metronidazole 18kg.
Preparation method and application are the same as embodiment 1.
Clinical practice
3 kinds of compositions are prepared first：
Composition 1：It prepares as described in Example 1；
Composition 2：It prepares as described in Example 2；
Composition 3：It prepares as described in Example 3；
Pilot production：
The healthy AA broiler chicken of 200 1 age in days non-ball worm infection is chosen, is randomly divided into 4 groups, every group 50.
Group one：Composition 1 is fed in 11-15 ages in days, 17-21 ages in days respectively, per 100g the composition spices 200kg.16
Age in days inoculation Eimeria Tenella egg capsule vaccine strain, 40,000 egg capsules/only.It collects the excrement of 22-25 ages in days and therefrom extracts ovum
Capsule.
Group two：Composition 2 is not fed in 11-15 ages in days, 17-21 ages in days, per 100g the composition spices 200kg.16 days
Age inoculation Eimeria Tenella egg capsule vaccine strain, 40,000 egg capsules/only.It collects the excrement of 22-25 ages in days and therefrom extracts ovum
Capsule.
Group three：Composition 3 is not fed in 11-15 ages in days, 17-21 ages in days, per 100g the composition spices 200kg.16 days
Age inoculation Eimeria Tenella egg capsule vaccine strain, 40,000 egg capsules/only.It collects the excrement of 22-25 ages in days and therefrom extracts ovum
Capsule.
Group four：It is normal to raise for blank control group, any drug is not fed.16 ages in days are inoculated with Eimeria Tenella ovum
Capsule vaccine strain, 40,000 egg capsules/only.It collects the excrement of 22-25 ages in days and therefrom extracts egg capsule.
Comparative result：
One egg capsule yield of group：43500000 egg capsules/chicken
Two egg capsule yield of group：39750000 egg capsules/chicken
Three egg capsule yield of group：42980000 egg capsules/chicken
Four egg capsule yield of group：32350000 egg capsules/chicken
Interpretation of result：The Eimeria Tenella egg capsule of 1,2,3 chicken of composition group is fed respectively than not feeding any drug
Chicken group egg capsule yield it is high by 34.5%, 22.9%, 32.9%.Data above illustrates that the present composition is improving tender Amy that
There is obvious action in coccidian oocyst yield, be greatly improved the production efficiency of Eimeria Tenella egg capsule, reduce coccidia epidemic disease
Seedling production cost.
Claims (3)
1. a kind of composition for improving Eimeria Tenella egg capsule yield and preparation method thereof, it is characterised in that：The composition
Component and its ratio of weight and number be：
20-50 parts of Cortex Phellodendri；
0.1-5 parts of propolis；
20-60 parts of the coptis；
10-20 parts of metronidazole.
2. a kind of composition for improving Eimeria Tenella egg capsule yield according to claim 1 and preparation method thereof,
It is characterized in that：The composition component and its optimal ratio of weight and number are respectively：
35 parts of Cortex Phellodendri；
0.2 part of propolis；
50 parts of the coptis；
14.8 parts of metronidazole.
3. a kind of a kind of composition for improving Eimeria Tenella egg capsule yield as described in claim 1 and its preparation side
Method, it is characterised in that：The step of preparation method is：
(1) Cortex Phellodendri is weighed respectively according to weight proportion, the coptis crushes and cross 60 mesh sieve, be put into mixing tank, mix 30 points
Clock is spare.
(2) weigh propolis according to weight proportion, crush and sieve with 100 mesh sieve, be put into equipped with Cortex Phellodendri, the coptis mixing tank in it is spare.
(3) according to weight proportion weigh metronidazole be put into equipped with Cortex Phellodendri, the coptis, propolis mixing tank in mix 30 minutes.Mixing
After uniformly in packing to aluminium foil bag, sealing.It can obtain a kind of composition for improving Eimeria Tenella egg capsule yield.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711493913.1A CN108148761A (en) | 2017-12-31 | 2017-12-31 | A kind of composition for improving Eimeria Tenella egg capsule yield and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711493913.1A CN108148761A (en) | 2017-12-31 | 2017-12-31 | A kind of composition for improving Eimeria Tenella egg capsule yield and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108148761A true CN108148761A (en) | 2018-06-12 |
Family
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| CN201711493913.1A Withdrawn CN108148761A (en) | 2017-12-31 | 2017-12-31 | A kind of composition for improving Eimeria Tenella egg capsule yield and preparation method thereof |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019940A (en) * | 2007-03-06 | 2007-08-22 | 广东省家禽科学研究所 | Organic composite Chinese medicine immunopotentiator/adjuvant and its prepn process |
| CN102727848A (en) * | 2012-06-20 | 2012-10-17 | 青岛绿曼生物工程有限公司 | Pure traditional Chinese medicine composition used for treating poultry infectious bursal disease, and preparation method thereof |
| CN102973828A (en) * | 2012-11-22 | 2013-03-20 | 青岛绿曼生物工程有限公司 | Compound propolis composition for treating poultry rabbit coccidiosis and preparation method thereof |
| EP2172481B1 (en) * | 2008-10-06 | 2014-10-29 | Novoplant GmbH | Proteolytically stable antibody formats |
| CN107019799A (en) * | 2016-01-30 | 2017-08-08 | 天津赫莱恩特生物科技有限公司 | A kind of application of chicken coccidiasis tetravalence attenuated live vaccines composition in production |
| CN107019111A (en) * | 2016-01-31 | 2017-08-08 | 天津赫莱恩特生物科技有限公司 | Improve composition of huge eimeria tenella egg capsule yield and preparation method thereof |
-
2017
- 2017-12-31 CN CN201711493913.1A patent/CN108148761A/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019940A (en) * | 2007-03-06 | 2007-08-22 | 广东省家禽科学研究所 | Organic composite Chinese medicine immunopotentiator/adjuvant and its prepn process |
| EP2172481B1 (en) * | 2008-10-06 | 2014-10-29 | Novoplant GmbH | Proteolytically stable antibody formats |
| CN102727848A (en) * | 2012-06-20 | 2012-10-17 | 青岛绿曼生物工程有限公司 | Pure traditional Chinese medicine composition used for treating poultry infectious bursal disease, and preparation method thereof |
| CN102973828A (en) * | 2012-11-22 | 2013-03-20 | 青岛绿曼生物工程有限公司 | Compound propolis composition for treating poultry rabbit coccidiosis and preparation method thereof |
| CN107019799A (en) * | 2016-01-30 | 2017-08-08 | 天津赫莱恩特生物科技有限公司 | A kind of application of chicken coccidiasis tetravalence attenuated live vaccines composition in production |
| CN107019111A (en) * | 2016-01-31 | 2017-08-08 | 天津赫莱恩特生物科技有限公司 | Improve composition of huge eimeria tenella egg capsule yield and preparation method thereof |
Non-Patent Citations (3)
| Title |
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| 李骁驽: "中草药在鸡病防治中的应用", 《兽药与饲料添加剂》 * |
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| 陈庆忠等: "常山提取物对人工感染的鸡球虫病疗效研究", 《现代农业科技》 * |
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| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180612 |
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