CN107019799A - A kind of application of chicken coccidiasis tetravalence attenuated live vaccines composition in production - Google Patents
A kind of application of chicken coccidiasis tetravalence attenuated live vaccines composition in production Download PDFInfo
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- CN107019799A CN107019799A CN201610064936.XA CN201610064936A CN107019799A CN 107019799 A CN107019799 A CN 107019799A CN 201610064936 A CN201610064936 A CN 201610064936A CN 107019799 A CN107019799 A CN 107019799A
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- tetravalence
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/002—Protozoa antigens
- A61K39/012—Coccidia antigens
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/70—Multivalent vaccine
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Abstract
The present invention relates to a kind of application of chicken coccidiasis tetravalence attenuated live vaccines composition in production, the component and its quantity portion rate of said composition are：Tender your 10-40 parts of coccidia of liking to be beautiful；Poison 10-30 parts of eimeria tenella；Huge eimeria tenella 5-20 parts；10-40 parts of E.acervulina；Inventive formulation composition is scientific and reasonable, and technique is simple, and easy to use, cost is relatively low, and the rate of animals delivered to the slaughter-house is high, regularity is good, feedstuff-meat ratio is low, aquaculture cost is low, profitable；Drug residue free, environmental protection.
Description
Technical field
The invention belongs to veterinary biologics field, especially a kind of chicken coccidiasis tetravalence attenuated live vaccines group
The application of compound in production.
Background technology
Chicken coccidiasis is the common disease in broiler breeding, is also to spend one of medicine cost highest disease,
And global-worm illness medicine is also to pollute environment, produce the main cause of medicament residue, coccidia expense is accounted for always
30 parts of medical expense a, chicken can all infect coccidia from be born to delivering for sale, and infection is usually in explosive stream
OK, it is classified as by United States Department of Agriculture and birds is endangered with one of five the most serious big diseases, chicken coccidiasis occurs
Afterwards, the production performance of chicken is influenceed, the price of deed is reduced, causes chick growth to be obstructed, serious makes
It is dead into a large amount of chickens, once outburst will result in the economic loss that can not be made up, current proper use of ball
Worm vaccine has achieved success, with the appearance of drug-resistant worm plant, is only got over by drug therapy global-worm illness way
Walk it is narrower, therefore control global-worm illness and treatment global-worm illness be broiler chicken obtain economic benefit key link.
The content of the invention
It is an object of the invention to provide a kind of chicken coccidiasis tetravalence attenuated live vaccines composition, the present invention matches somebody with somebody
Fang Zucheng is scientific and reasonable, technique is simple, easy to use, cost is relatively low, the rate of animals delivered to the slaughter-house is high, regularity good,
Feedstuff-meat ratio and aquaculture cost are low；Drug residue free, protection of the pole beneficial to environment.
It is another object of the present invention to provide a kind of system of chicken coccidiasis tetravalence attenuated live vaccines composition
Preparation Method.
The present invention is achieved by the following technical solutions：
A kind of chicken coccidiasis tetravalence attenuated live vaccines composition, the component and its quantity portion rate of its composition
For：
Moreover, said composition component and its optimal number portion rate are respectively：
The step of a kind of preparation method of chicken coccidiasis tetravalence attenuated live vaccines composition, its preparation method is：
(1) with blood counting chamber by Sporulated Eimeria Tenella, poison eimeria tenella, huge Amy
That coccidia, E.acervulina are counted, and count each component coccidia worm according to quantitative proportion respectively
Strain number amount, is sequentially placed into same clean container, must mix coccidia worm strain A；
(2) added into mixing coccidia worm strain A obtained by step (1) and preserve liquid and be stirred, must mixed
Liquid B；
(3) mixed liquid B being stirred obtained by step (2) is dispensed in superclean bench, every bottle
1000 plumages part, you can obtain a kind of tetravalence coccidia attenuated live vaccines composition.
Advantages of the present invention and beneficial effect are：
1. the present invention obtains the strain of virulence is small, immunogenicity is good worm by Cultivation of Precocity Strain, reduce schizogamy,
The good immunogenicity of holding, reduction clinical vaccine adverse reaction, use safety；
2. inventive formulation composition is scientific and reasonable, technique is simple, and easy to use, cost is relatively low, can be effective
The immunity of broiler chicken coccidia is improved, chicken group is set up powerful be immunized after attacking poison by an immune cycle
System.
3. four kinds of coccidia Sporulated Oocysts in the present invention are all clinical common coccidia disease pest strains, 99% can be reached
Protective rate, increase body tolerance.
Embodiment
The present invention is described in further detail by following examples.It should be noted that：Following embodiments are explanations
Property, it is not limited, it is impossible to limit protection scope of the present invention with following embodiments.
Sporulated Eimeria Tenella, murder by poisoning eimeria tenella, huge Amy that in instant component
Coccidia, E.acervulina are by the pure egg capsule obtained after healthy SPF chicken inoculations, without other
Miscellaneous egg capsule.
Embodiment 1
A kind of chicken coccidiasis tetravalence attenuated live vaccines composition, the component and its quantity of its composition are respectively：
Tender your coccidia 20000 of liking to be beautiful；Poison eimeria tenella 20000；Huge Chinese mugwort so-so coccidia 10000
It is individual；E.acervulina 20000.
The step of a kind of preparation method of chicken coccidiasis tetravalence attenuated live vaccines composition, its preparation method is：
(1) with blood counting chamber by Sporulated Eimeria Tenella, poison eimeria tenella, huge Chinese mugwort
Mei Er coccidias, E.acervulina are counted respectively, and count each component coccidia according to quantitative proportion
Worm strain number amount, is sequentially placed into same clean container, must mix coccidia worm strain A；
(2) added into mixing coccidia worm strain A obtained by step (1) and preserve liquid and be stirred, must mixed
Liquid B；
(3) mixed liquid B being stirred obtained by step (2) is dispensed in superclean bench, every bottle
1000 plumages part, you can obtain a kind of tetravalence coccidia attenuated live vaccines composition.
Embodiment 2
A kind of chicken coccidiasis tetravalence attenuated live vaccines composition, the component and its quantity of its composition are respectively：
Tender your coccidia 40000 of liking to be beautiful；Poison eimeria tenella 30000；Huge Chinese mugwort so-so coccidia 20000
It is individual；E.acervulina 40000.
The step be the same as Example 1 of its preparation method.
Embodiment 3
A kind of chicken coccidiasis tetravalence attenuated live vaccines composition, the component and its quantity of its composition are respectively：
Tender your coccidia 20000 of liking to be beautiful；Poison eimeria tenella 20000；Huge Chinese mugwort so-so coccidia 20000
It is individual；E.acervulina 20000.
The step be the same as Example 1 of its preparation method.
Clinical practice
The honest broiler breeding company in test site Qinhuangdao, Ai Wei mattress SPF broiler chicken 60 5 Japanese instar chicklings point
Into 3 groups, every group 20, be put into isolator and raise.A groups are sick for the tetravalence chicken ball of the positive allusion quotation in Foshan
Live vaccine is that tetravalence Eimeria species live vaccine group of the present invention, C groups are blank group using group, B groups, during packet by
Only weigh, each group distribution uniform in size.Vaccine selects the tetravalence chicken ball disease live-vaccine 1 of the positive allusion quotation in Foshan
Plumage part/only, of the present invention group is also that a 1 plumage part/progress drinking-water is immune；C groups only attack poison and do not do immune；With
A malicious 50000 egg capsule/chicken is attacked to three groups of progress respectively within the 8th day after complete vaccine, attack after poison the 4th day to young bird
Chicken is cooked clinical observation, and A groups attack part chicken group excrement after poison and bloody stool, tomato sample excrement, indivedual chickens occur
Loose random, lassitude, anorexia；B group chickens are attacked after poison, only one tomato sample excrement
Just, bloody stool is had no；C groups attack full group after poison and bloody stool, tomato sample excrement, feed intake occur and largely reduce,
There are the chicken phenomena of mortality.Excrement OPG and chicken group's protective rate are recorded, and is recorded in detail.(refer to examination
Test contrast table)
Watch test contrast table
| Group | A groups | B groups | C groups |
| The age of immune | 5 ages in days | 5 ages in days | 5 ages in days |
| Quantity | 20 | 20 | 20 |
| Average weight | 127 grams/only | 125 grams/only | 126 grams/only |
| Immunization route | Drinking-water is immune | Drinking-water is immune | // |
| Immunizing dose | 1 plumage part/only | 1 plumage part/only | // |
| Chicken group state after immune | Feed intake is reduced | No abnormality seen | // |
| Immune protective rate | 96.3 parts | 99.2 parts | // |
| Attack toxic agent amount | 50000 egg capsules/only | 50000 egg capsules/only | 50000 egg capsules/only |
| OPG | 2854/gram | 128/gram | 0 |
| Attack protective rate after poison | 95 parts | 100 parts | 62 parts |
Summarize：Show that tetravalence chicken coccidiasis attenuated live vaccines of the present invention make in clinic by above-mentioned conclusion (of pressure testing)
The protective rate used is higher than the protective rate of the existing coccidial vaccine in market, and the present invention maintains immunogenicity
Vaccine virulence in itself and adverse reaction are reduced again.
Claims (3)
1. a kind of application of chicken coccidiasis tetravalence attenuated live vaccines composition in production, it is special
Levy and be：The component and its quantity portion rate of said composition be：
2. a kind of chicken coccidiasis tetravalence attenuated live vaccines composition according to claim 1,
It is characterized in that：Said composition component and its optimal number portion rate are respectively：
3. a kind of a kind of chicken coccidiasis tetravalence attenuated live vaccines combination as claimed in claim 1
The preparation method of thing, it is characterised in that：The step of preparation method is：
(1) with blood counting chamber by Sporulated Eimeria Tenella, poison eimeria tenella,
Huge eimeria tenella, E.acervulina are counted respectively, and according to quantitative proportion number
Go out each component coccidia worm strain number amount, be sequentially placed into same clean container, coccidia worm must be mixed
Strain A；
(2) added into mixing coccidia worm strain A obtained by step (1) and preserve liquid and be stirred,
Obtain mixed liquid B；
(3) mixed liquid B being stirred obtained by step (2) is divided in superclean bench
Dress, every bottle of 1000 plumages part, you can obtain a kind of tetravalence coccidia attenuated live vaccines composition.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610064936.XA CN107019799A (en) | 2016-01-30 | 2016-01-30 | A kind of application of chicken coccidiasis tetravalence attenuated live vaccines composition in production |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610064936.XA CN107019799A (en) | 2016-01-30 | 2016-01-30 | A kind of application of chicken coccidiasis tetravalence attenuated live vaccines composition in production |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108148761A (en) * | 2017-12-31 | 2018-06-12 | 天津赫莱恩特生物科技有限公司 | A kind of composition for improving Eimeria Tenella egg capsule yield and preparation method thereof |
Citations (6)
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|---|---|---|---|---|
| WO2003099325A1 (en) * | 2002-05-21 | 2003-12-04 | Schering-Plough Ltd. | Methods for the in vitro culture of sporozoea sp. and user thereof |
| CN100998866A (en) * | 2006-01-09 | 2007-07-18 | 佛山市正典生物技术有限公司 | Preparation method of chicken coccidiosis fetra valence active vaccine composed from medicine sensitive insect plant |
| WO2008118255A1 (en) * | 2007-02-08 | 2008-10-02 | Embrex, Inc. | Methods of releasing sporocysts from oocysts using controlled shear forces |
| WO2009148895A1 (en) * | 2008-05-29 | 2009-12-10 | Intervet International B.V. | Coccidiosis vaccines |
| CN103386124A (en) * | 2013-07-01 | 2013-11-13 | 南京农业大学 | Multivalent recombinant protein subunit vaccine in chicken coccidiosis and application thereof |
| CN103710265A (en) * | 2013-12-23 | 2014-04-09 | 广东省农业科学院动物卫生研究所 | Application of chloramine B solution in chicken coccidiosis oocyst culture and preservation |
-
2016
- 2016-01-30 CN CN201610064936.XA patent/CN107019799A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003099325A1 (en) * | 2002-05-21 | 2003-12-04 | Schering-Plough Ltd. | Methods for the in vitro culture of sporozoea sp. and user thereof |
| CN100998866A (en) * | 2006-01-09 | 2007-07-18 | 佛山市正典生物技术有限公司 | Preparation method of chicken coccidiosis fetra valence active vaccine composed from medicine sensitive insect plant |
| WO2008118255A1 (en) * | 2007-02-08 | 2008-10-02 | Embrex, Inc. | Methods of releasing sporocysts from oocysts using controlled shear forces |
| WO2009148895A1 (en) * | 2008-05-29 | 2009-12-10 | Intervet International B.V. | Coccidiosis vaccines |
| CN103386124A (en) * | 2013-07-01 | 2013-11-13 | 南京农业大学 | Multivalent recombinant protein subunit vaccine in chicken coccidiosis and application thereof |
| CN103710265A (en) * | 2013-12-23 | 2014-04-09 | 广东省农业科学院动物卫生研究所 | Application of chloramine B solution in chicken coccidiosis oocyst culture and preservation |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108148761A (en) * | 2017-12-31 | 2018-06-12 | 天津赫莱恩特生物科技有限公司 | A kind of composition for improving Eimeria Tenella egg capsule yield and preparation method thereof |
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