CN108041034B - Attractant for female thrips tea rod gathering pheromone and method for preparing attractant core of attractant - Google Patents

Abstract

The invention discloses a tea stick thrips female pheromone attractant, comprising 2,4,7,9-tetramethyl-5-decyne-4,7-diol and a synergist, 2,4, The volume fraction of 7,9-tetramethyl-5-decyne-4,7-diol is 3.2-160 parts; The synergist is the mixture of the compounds of the following volume fractions: dimethyl glutarate 0.2 ‑60 parts; 0.2‑60 parts of lavender acetate, the tea stick thrips female aggregation pheromone attractant of the present invention, combined with the lure core made of the rubber stopper slow-release carrier, can effectively treat the tea sticks in the tea garden. The female thrips are lured. Combined with the lure color plate, it can play the role of ecologically regulating the population of thrips females in tea sticks. The lure core uses a slow-release carrier with a rubber stopper, so that the active ingredients can be released slowly and evenly, extending the attractant. The effective trapping time in the tea garden, the structure of each component in the attractant is stable, the tea stick thrips female pheromone attractant of the present invention has broad industrialization prospects, because the preparation method is simple and the cost is low, it is suitable for tea gardens. growers for promotion and application.

Description

A kind of tea stick thrips female worm aggregation pheromone attractant and its lure core preparation backup method

technical field

The invention relates to the technical field of chemical ecological control of pests, in particular to a tea stick thrips female pheromone attractant and a method for preparing a lure core of the attractant.

Background technique

Dendrothrips minowai Priesner belongs to the order Thysanoptera, Thripidae, Dendrothrips. The insect was originally recorded in Japan. Since it was discovered in Shuangliu Tea Farm in Kaiyang, Guizhou in 1990, it has now occurred in Zhejiang, Fujian, Guangdong, Guangxi, Hainan, Yunnan, Jiangsu, Jiangxi, Chongqing, Chengdu, Hunan and other places. It has a wide range of hosts, mainly harming tea, camellia, rouge, and lotus. The sap of the young buds and leaves of the tea tree is sucked by the adult and nymph files. The affected leaves become hard, lose their luster, and the leaves are coarse and old. The growth of the buds and leaves is stagnant. In severe cases, the affected leaves fall off. The damage caused by this pest is more than 50%, and the harvest is severe in severe cases. At present, chemical pesticides are mainly used to control the tea stick thrips, such as chlorfenapyr, indoxacarb, fenflunom, bifenthrin, and beta-cyhalothrin. Due to the long-term use of the above several pesticides, the resistance of tea stick thrips has increased, not only the control effect is not ideal, but also excessive application of chemical pesticides leads to problems such as excessive pesticide residues in tea leaves, affecting the health of tea consumers. In recent years, physical control methods have been favored after the recognition of the adverse effects of chemical pesticides. Yellow sticky insect boards and trap lights have also been gradually applied in tea gardens, but it is found that there are some drawbacks in physical control. It mainly traps and kills adults of large insects such as Lepidoptera and Coleoptera, but has almost no trapping effect on small thrips insects of Thysanoptera, and the cost is relatively high. Therefore, it is an inevitable trend to reduce the level of pesticide residues in tea and ensure the quality and safety of tea to develop an efficient and non-polluting green prevention and control technology based on the biological characteristics of thrips tea sticks and reduce the dosage of chemical pesticides.

Aggregation pheromones is a kind of insect pheromones, which are trace chemicals produced by insects and can cause the aggregation behavior of female and male insects of the same species. It also has the effect of gathering adults of both sexes to inhabit and feed together. As an important discovery in chemical ecology in the 1960s, insect sex pheromone has been recognized by scientists from all over the world because of its high efficiency, environmental protection, and strong specificity. It is considered as a "biological rational pesticide". So far, there are still few studies at home and abroad on the identification and biological activity of thrips aggregation pheromone components. Only the aggregation pheromones released by male thrips Frankliniella occidentalis, F. intonsa, Thri pspalmi and Echinothrips americanus were effectively isolated and identified. The control of flower thrips aggregation pheromone has become a model for the application of insect aggregation pheromone. Therefore, it is a green and efficient prevention and control measure with a very promising application prospect to study the aggregation pheromone of thrips thrips females and carry out the research and development of related application technologies.

In the study, the applicant used SPME to extract the volatile substances released by the female thrips tea stick, and used gas chromatography-mass spectrometry (GC-MS) to identify the active components of the volatile substances. The artificially synthesized aggregated pheromone compounds are prepared into lure cores in Yuqing County Yulong Tea Industry Co., Ltd., Guiyang Qianzhong Tea Garden Base Variety Resource Garden, Laoluopo Tea Farm, Xixiu District, Anshun City, Meitan County Luohuatun Tea Professional Cooperative, The Meitan Experimental Demonstration Base of the Provincial Tea Institute conducted field trapping experiments. The field lure test showed that the attractant formula has a significant attracting effect on the female thrips of tea stick. In view of this, the present invention is proposed in order to provide an effective method for population monitoring and control of female thrips tea sticks.

SUMMARY OF THE INVENTION

The technical problem to be solved by the present invention is to overcome the existing defects, and provide a technical scheme of an attractant that can specifically attract female thrips tea sticks and a method for preparing the lure core of the attractant. The aggregation pheromone in the volatiles of tea stick thrips females is the component. The artificial compound simulates the aggregation pheromone of tea stick thrips females, which can attract a large number of tea stick thrips females, and have a negative effect on the tea stick thrips females. The population monitoring and control of insects has a wide application prospect, and is pollution-free, residue-free, and environmentally friendly, and can effectively solve the problems in the background technology.

In order to achieve the above purpose, the present invention provides the following technical solutions: a tea stick thrips female pheromone attractant, comprising 2,4,7,9-tetramethyl-5-decyne-4,7-diol and Synergist, the volume fraction of the 2,4,7,9-tetramethyl-5-decyne-4,7-diol is 3.2-160 parts, and the synergist includes but is not limited to the following The mixture of compounds in the volume parts: 0.2-60 parts of dimethyl glutarate; 0.2-60 parts of lavender acetate.

As a preferred technical solution of the present invention, the synergist comprises a mixture of the following compounds in parts by volume: 0.2-60 parts of dimethyl glutarate, 0.2-60 parts of lavender acetate, verbene Ketone 0.2-60 parts.

As a preferred technical solution of the present invention, the synergist comprises a mixture of the following compounds in parts by volume: 0.2-60 parts of dimethyl glutarate, 0.2-60 parts of lavender acetate, verbene 0.2-60 parts of ketone, 0.2-60 parts of phthalic acid monoester.

As a preferred technical solution of the present invention, the synergist comprises a mixture of the following compounds in parts by volume: 0.2-60 parts of dimethyl glutarate, 0.2-60 parts of lavender acetate, verbene 0.2-60 parts of ketone, 0.2-60 parts of phthalic acid monoester, 0.2-60 parts of cis-3-hexen-1-ol.

A kind of preparation method of tea stick thrips female worm aggregation pheromone attractant lure core, it is characterized in that comprising the following process steps:

1) Preparation of sustained-release carrier:

Choose a rubber stopper with a diameter of 8-10mm, soak it in absolute ethanol for 24-48h, take out the rubber stopper and put it in a 30-50℃ oven for 24-48h;

2) Preparation of aggregation pheromone attractant: measure each component according to the above-mentioned formula, add in organic solvents such as n-hexane or paraffin oil and mix well, for subsequent use;

3) Add the attractant prepared in step 2) dropwise to the slow-release carrier prepared in step 1), and then let stand for 1-5 hours at 10-50° C., to obtain a tea stick thrips female worm aggregation pheromone attractant. Agent lure.

In the present invention, the molecular formula of 2,4,7,9-tetramethyl-5-decyne-4,7-diol: C ₁₄ H ₂₆ O ₂ , the molecular formula of lavender acetate: C ₁₂ H ₂₀ O ₂ , the Dimethyl diacid Molecular formula: C ₇ H ₁₂ O ₄ , Verbenone Molecular formula: C ₁₀ H ₁₄ O, Phthalic acid monoester Molecular formula: C ₉ H ₈ O ₄ , cis-3-hexene-1- Molecular formula of alcohol: C ₆ H ₁₂ O.

Compared with the prior art, the beneficial effects of the present invention are as follows: the tea stick thrips female pheromone attractant of the present invention, combined with the lure core made of a rubber stopper slow-release carrier, can effectively treat the tea sticks in the tea garden. The female thrips are lured. Combined with the lure color plate, it can play the role of ecologically regulating the population of thrips females in tea sticks. The lure core uses a slow-release carrier with a rubber stopper, so that the active ingredients can be released slowly and evenly, extending the attractant. The effective trapping time in the tea garden, the structure of each component in the attractant is stable, the tea stick thrips female pheromone attractant of the present invention has broad industrialization prospects, because the preparation method is simple and the cost is low, it is suitable for tea gardens. growers for promotion and application.

Detailed ways

The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

Example 1

A kind of preparation method of tea stick thrips female worm aggregation pheromone attractant lure core, the concrete steps are as follows:

1) Preparation of sustained-release carrier: choose a rubber stopper with a diameter of 8 mm, soak it in absolute ethanol for 24 hours, take out the rubber stopper and place it in a 30°C oven for 24 hours;

2) Preparation of attractant: measure each component of attractant according to the following formula

Take 2,4,7,9-tetramethyl-5-decyne-4,7-diol 3.2μL/each lure core, lavender acetate 0.2μL/each lure core, dimethyl glutarate Ester 0.2 μL/each lure, verbenone 0.2 μL/each lure, phthalate monoester 0.2 μL/each lure, cis-3-hexen-1-ol 0.2 μL/each To lure the core, add n-hexane or paraffin oil and other organic solvents, mix well, set aside;

3) Add the attractant prepared in step 2) dropwise to the slow-release carrier prepared in step 1), and then stand at 10° C. for 1 hour, to obtain a pheromone attractant attractant for female thrips thrips.

Example 2

A kind of preparation method of tea stick thrips female worm aggregation pheromone attractant lure core, the concrete steps are as follows:

1) Preparation of sustained-release carrier: choose a rubber stopper with a diameter of 8 mm, soak it in absolute ethanol for 24 hours, take out the rubber stopper and place it in a 40°C oven for 24 hours;

2) Preparation of attractant: measure each component of attractant according to the following formula

Take 2,4,7,9-tetramethyl-5-decyne-4,7-diol 25μL / each lure core, lavender acetate 2μL / each lure core, dimethyl glutarate 2μL /Each lure, verbenone 2μL/each lure, phthalate monoester 2μL/each lure, cis-3-hexen-1-ol 2μL/each lure, add n-hexane Or evenly mixed in an organic solvent such as paraffin oil, and set aside;

3) Add the attractant prepared in step 2) dropwise to the slow-release carrier prepared in step 1), and then stand at 20° C. for 2 hours, to prepare a pheromone attractant attractant for female thrips tea sticks.

Example 3

A kind of preparation method of tea stick thrips female worm aggregation pheromone attractant lure core, the concrete steps are as follows:

1) Preparation of sustained-release carrier: choose a rubber stopper with a diameter of 8 mm, soak it in absolute ethanol for 36 hours, take out the rubber stopper and place it in a 40°C oven for 24 hours;

2) Preparation of attractant: measure each component of attractant according to the following formula

Take 2,4,7,9-tetramethyl-5-decyne-4,7-diol 40μL/each lure core, lavender acetate 4μL/each lure core, dimethyl glutarate 4μL /Each lure, verbenone 4μL/each lure, phthalate monoester 4μL/each lure, cis-3-hexen-1-ol 4μL/each lure, add n-hexane Or evenly mixed in an organic solvent such as paraffin oil, and set aside;

3) Add the attractant prepared in step 2) dropwise to the slow-release carrier prepared in step 1), and then stand at 20° C. for 2 hours, to prepare a pheromone attractant attractant for female thrips tea sticks.

Example 4

A kind of preparation method of tea stick thrips female worm aggregation pheromone attractant lure core, the concrete steps are as follows:

1) Preparation of sustained-release carrier: choose a rubber stopper with a diameter of 10 mm, soak it in absolute ethanol for 48 hours, take out the rubber stopper and place it in a 40°C oven for 36 hours;

2) Preparation of attractant: measure each component of attractant according to the following formula

Take 2,4,7,9-tetramethyl-5-decyne-4,7-diol 60μL / each lure core, lavender acetate 6μL / each lure core, dimethyl glutarate 6μL / each lure, verbenone 6 μL / each lure, phthalate monoester 6 μL / each lure, cis-3-hexen-1-ol 6 μL / each lure add n-hexane or paraffin Mix evenly in organic solvents such as oil, and set aside;

3) Add the attractant prepared in step 2) dropwise to the slow-release carrier prepared in step 1), and then stand at 40° C. for 4 hours to obtain a pheromone attractant attractant for female thrips thrips.

Example 5

A kind of preparation method of tea stick thrips female worm aggregation pheromone attractant lure core, the concrete steps are as follows:

1) Preparation of sustained-release carrier: choose a rubber stopper with a diameter of 10 mm, soak it in absolute ethanol for 48 hours, take out the rubber stopper and place it in a 50°C oven for 48 hours;

2) Preparation of attractant: measure each component of attractant according to the following formula

Take 2,4,7,9-tetramethyl-5-decyne-4,7-diol 80μL/each lure core, lavender acetate 10μL/each lure core, dimethyl glutarate 10μL /Each lure, verbenone 10μL/each lure, phthalate monoester 10μL/each lure, cis-3-hexen-1-ol 10μL/each lure, add n-hexane Or evenly mixed in an organic solvent such as paraffin oil, and set aside;

3) Add the attractant prepared in step 2) dropwise to the slow-release carrier prepared in step 1), and then stand at 50° C. for 5 hours, to obtain a pheromone attractant attractant for female thrips thrips.

Example 6

A kind of preparation method of tea stick thrips female worm aggregation pheromone attractant lure core, the concrete steps are as follows:

1) Preparation of sustained-release carrier: choose a rubber stopper with a diameter of 10 mm, soak it in absolute ethanol for 48 hours, take out the rubber stopper and place it in a 50°C oven for 48 hours;

2) Preparation of attractant: measure each component of attractant according to the following formula

Take 2,4,7,9-tetramethyl-5-decyne-4,7-diol 100μL/each lure, lavender acetate 12μL/each lure, dimethyl glutarate 12μL /Each lure, verbenone 12μL/each lure, phthalate monoester 12μL/each lure, cis-3-hexen-1-ol 12μL/each lure, add n-hexane Or evenly mixed in an organic solvent such as paraffin oil, and set aside;

3) Add the attractant prepared in step 2) dropwise to the slow-release carrier prepared in step 1), and then stand at 50° C. for 5 hours, to obtain a pheromone attractant attractant for female thrips thrips.

Example 7

A kind of preparation method of tea stick thrips female worm aggregation pheromone attractant lure core, the concrete steps are as follows:

1) Preparation of sustained-release carrier: choose a rubber stopper with a diameter of 10 mm, soak it in absolute ethanol for 48 hours, take out the rubber stopper and place it in a 50°C oven for 48 hours;

2) Preparation of attractant: measure each component of attractant according to the following formula

Take 2,4,7,9-tetramethyl-5-decyne-4,7-diol 120μL/each lure, lavender acetate 20μL/each lure, dimethyl glutarate 20μL / each lure, verbenone 20 μL/ each lure, phthalate monoester 20 μL/ each lure, cis-3-hexen-1-ol 20 μL/ each lure, add n-hexane Or evenly mixed in an organic solvent such as paraffin oil, and set aside;

3) Add the attractant prepared in step 2) dropwise to the slow-release carrier prepared in step 1), and then stand at 50° C. for 5 hours, to obtain a pheromone attractant attractant for female thrips thrips.

Example 8

A kind of preparation method of tea stick thrips female worm aggregation pheromone attractant lure core, the concrete steps are as follows:

1) Preparation of sustained-release carrier: choose a rubber stopper with a diameter of 10 mm, soak it in absolute ethanol for 48 hours, take out the rubber stopper and place it in a 50°C oven for 48 hours;

2) Preparation of attractant: measure each component of attractant according to the following formula

Take 2,4,7,9-tetramethyl-5-decyne-4,7-diol 140μL/each lure core, lavender acetate 40μL/each lure core, dimethyl glutarate 40μL /Each lure, verbenone 40μL/each lure, phthalate monoester 40μL/each lure, cis-3-hexen-1-ol 40μL/each lure, add n-hexane Or evenly mixed in an organic solvent such as paraffin oil, and set aside;

3) Add the attractant prepared in step 2) dropwise to the slow-release carrier prepared in step 1), and then stand at 50° C. for 5 hours, to obtain a pheromone attractant attractant for female thrips thrips.

Example 9

A kind of preparation method of tea stick thrips female worm aggregation pheromone attractant lure core, the concrete steps are as follows:

1) Preparation of sustained-release carrier: choose a rubber stopper with a diameter of 10 mm, soak it in absolute ethanol for 48 hours, take out the rubber stopper and place it in a 50°C oven for 48 hours;

2) Preparation of attractant: measure each component of attractant according to the following formula

Take 2,4,7,9-tetramethyl-5-decyne-4,7-diol 160μL / each lure, lavender acetate 60μL / each lure, dimethyl glutarate 60μL / For each lure, add it into an organic solvent such as n-hexane or paraffin oil and mix it evenly, then mix it evenly for later use;

3) Add the attractant prepared in step 2) dropwise to the slow-release carrier prepared in step 1), and then stand at 50° C. for 5 hours, to obtain a pheromone attractant attractant for female thrips thrips.

Example 10

A kind of preparation method of tea stick thrips female worm aggregation pheromone attractant lure core, the concrete steps are as follows:

1) Preparation of sustained-release carrier: choose a rubber stopper with a diameter of 10 mm, soak it in absolute ethanol for 48 hours, take out the rubber stopper and place it in a 50°C oven for 48 hours;

2) Preparation of attractant: measure each component of attractant according to the following formula

Take 2,4,7,9-tetramethyl-5-decyne-4,7-diol 160μL / each lure, lavender acetate 60μL / each lure, dimethyl glutarate 60μL /Each lure core, verbenone 60μL/each lure core, add n-hexane or paraffin oil and other organic solvent to mix well, set aside;

3) Add the attractant prepared in step 2) dropwise to the slow-release carrier prepared in step 1), and then stand at 50° C. for 5 hours, to obtain a pheromone attractant attractant for female thrips thrips.

Example 11

A kind of preparation method of tea stick thrips female worm aggregation pheromone attractant lure core, the concrete steps are as follows:

1) Preparation of sustained-release carrier: choose a rubber stopper with a diameter of 10 mm, soak it in absolute ethanol for 48 hours, take out the rubber stopper and place it in a 50°C oven for 48 hours;

2) Preparation of attractant: measure each component of attractant according to the following formula

Take 2,4,7,9-tetramethyl-5-decyne-4,7-diol 160μL / each lure, lavender acetate 60μL / each lure, dimethyl glutarate 60μL /Each lure, verbenone 60μL/each lure, phthalate monoester 60μL/each lure, add n-hexane or paraffin oil and other organic solvent to mix well, set aside;

3) Add the attractant prepared in step 2) dropwise to the slow-release carrier prepared in step 1), and then stand at 50° C. for 5 hours, to obtain a pheromone attractant attractant for female thrips thrips.

Test Example 1

The test was carried out in the first peak period of the tea stick thrips female worm in the tea garden (in June), and the location was the tea base of Yulong Tea Industry Co., Ltd., Yuqing County, Guizhou Province, and the tea stick thrips obtained in Example 1-11 was used in the test. Female worms gather pheromone attractant lure core, the lure core is combined with the lure color plate as the test treatment, the lure color plate with the blank lure core is used as a blank control, and the treatment and control lure color plate are investigated every 2 days after the start of the test. Number of tea stick thrips females. The treatments and controls were randomly arranged in the field, the spacing between the trapping color plates was 6 meters, and the treatments and controls were 4 replicates.

From the survey data in Table 1, it can be seen that the attracting ability of the attractant lure core combined with the lure color plate to the tea stick thrips female is higher than that of the blank lure core. , After the ninth day, the attracting rate to the female tea stick thrips had a very significant difference between the attractant and the blank control. It shows that the tea stick thrips female worm aggregation pheromone attractant formula in the present invention has obvious attracting effect.

Table 1 Attractive effect of attractant combined with lure color palette on female tea stick thrips (mean ± standard error)

Note: The data are the mean ± standard error. Different lowercase English letters after the data in the same column indicate significant differences at the 0.05 level by one-way ANOVA.

Test Example 2

The test was carried out in the first peak period of the tea stick thrips female worm in the tea garden (in June), and the location was the tea base of Luohuatun Tea Professional Cooperative, Meitan County, Guizhou Province. The tea stick thrips obtained in Examples 1-8 were used in the test. Females gather pheromone attractant lure cores, the lure cores are combined with the lure color plate as the test treatment, and the lure color plate with the blank lure core is used as a blank control. Number of tea stick thrips females. The treatments and controls were randomly arranged in the field, the spacing between the trapping color plates was 6 meters, and the treatments and controls were 4 replicates.

From the survey data in Table 2, it can be seen that the number of tea stick thrips females on the lure color plate with the attractant lure core is higher than that of the blank control. After 9 days, there was a very significant difference between the relative numbers of tea stick thrips females on the attractant-centred bait panels and the blank control. This shows that the tea stick thrips female worm aggregation pheromone attractant formula in the present invention has a good attracting effect on the tea stick thrips female worms.

Table 2 Attraction effect of attractant combined with lure color palette on female tea stick thrips (mean ± SE)

Note: The data are the mean ± standard error. Different lowercase English letters after the data in the same column indicate significant differences at the 0.05 level by one-way ANOVA.

Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, and substitutions can be made in these embodiments without departing from the principle and spirit of the invention and modifications, the scope of the present invention is defined by the appended claims and their equivalents.

Claims (5)

1. A tea stick thrips female worm aggregation pheromone attractant, comprising 2,4,7,9-tetramethyl-5-decyne-4,7-diol and a synergist, characterized in that: the described The volume fraction of 2,4,7,9-tetramethyl-5-decyne-4,7-diol is 3.2-160 parts by volume, and the synergist comprises a mixture of compounds in the following volume fractions: 0.2-60 parts of dimethyl diacid; 0.2-60 parts of lavender acetate. 2. a kind of tea stick thrips female worm aggregation pheromone attractant according to claim 1, is characterized in that: described synergist comprises the mixture of the compound of following parts by volume: dimethyl glutarate 0.2 -60 parts, 0.2-60 parts of lavender acetate, 0.2-60 parts of verbenone. 3. a kind of tea stick thrips female worm aggregation pheromone attractant according to claim 1, is characterized in that: described synergist comprises the mixture of the compound of following parts by volume: dimethyl glutarate 0.2 -60 parts, lavender acetate 0.2-60 parts, verbenone 0.2-60 parts, phthalic acid monoester 0.2-60 parts. 4. a kind of tea stick thrips female worm aggregation pheromone attractant according to claim 1, is characterized in that: described synergist comprises the mixture of the compound of following parts by volume: dimethyl glutarate 0.2 -60 parts, lavender acetate 0.2-60 parts, verbenone 0.2-60 parts, phthalic acid monoester 0.2-60 parts, cis-3-hexen-1-ol 0.2-60 parts. 5. a preparation method of tea stick thrips female worm aggregation pheromone attractant lure core, is characterized in that, comprises the following processing steps: 1) Preparation of sustained-release carrier: Choose a rubber stopper with a diameter of 8-10mm, soak it in absolute ethanol for 24-48h, take out the rubber stopper and put it in a 30-50℃ oven for 24-48h; 2) the preparation of aggregation pheromone attractant: measure each component according to the formula described in claim 1, add in normal hexane or paraffin oil and mix well, obtain attractant, standby; 3) Add the attractant prepared in step 2) dropwise to the slow-release carrier prepared in step 1), and then let stand for 1-5 hours at 10-50° C., to obtain a tea stick thrips female worm aggregation pheromone attractant. Agent lure.