CN108017445A - A kind of reclamation of salinep-alkali soil type microbial bacterial agent and preparation method thereof - Google Patents
A kind of reclamation of salinep-alkali soil type microbial bacterial agent and preparation method thereof Download PDFInfo
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- CN108017445A CN108017445A CN201711271018.5A CN201711271018A CN108017445A CN 108017445 A CN108017445 A CN 108017445A CN 201711271018 A CN201711271018 A CN 201711271018A CN 108017445 A CN108017445 A CN 108017445A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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Abstract
The invention discloses a kind of reclamation of salinep-alkali soil type microbial bacterial agent and preparation method thereof, the parts by weight composition of microbial bacterial agent is as follows：10~30 parts of bacillus megaterium HB bacterium powders, 35~65 parts of phosphorus mine tailing, 12~18 parts of ardealite, 8~25 parts of sugar refinery waste residue, 6~26 parts of seaweed slag, 1~8 part of mannitol.Each component is synergistic in reclamation of salinep-alkali soil type microbial bacteria agent prescription of the present invention, lack any type, all fall flat, in the case of only using reclamation of salinep-alkali soil type microbial bacterial agent full formula of the present invention, it can be played under conditions of 30~80 kilograms of usage amount per acre and improve utilization rate of fertilizer, crop yield, the effect for improving quality, improveing salt-soda soil well.
Description
Technical field
The present invention relates to a kind of reclamation of salinep-alkali soil type microbial bacterial agent and preparation method thereof, belong to technical field of fertilizers.
Background technology
Saline-alkali soil is the general name of solonchak and alkaline earth, and saline Land is due to unreasonable irrigation and the chemical fertilizer in farmland more
Caused by excessive use.Since the content of organic matter of saline-alkali soil is few, the soil salinization is serious, and soil fertility is low, and physicochemical character is poor,
Generally lack phosphorus and lack nitrogen, the anions and canons being harmful to crop are more, cause crop to be not easy to emerge, seriously affected the quality of crop with
Yield.The distribution of Chinese saline-alkali soil is wider, can carry out nearly 1,300,000 hm of saline and alkaline land area of agricultural use2, account for Chinese arable land
More than the 10% of area, potentiality to be exploited is huge.As population increases, Cultivated Land Area Decrease, the improvement and improvement in salt-soda soil are also got over
To be more subject to people to pay attention to.
The improvement of saline-alkali soil mainly includes engineering improvement, chemical modifying and biological modification.Engineering improvement short run effect is obvious,
But project inputs are big；Chemical modifying is quick, but of high cost, and side effect is obvious.Bioanalysis improvement has that cost is low, operation side
Just the advantages that secondary pollution, is not produced, therefore, is had broad prospects using the research in bioanalysis improvement salt-soda soil.It is more at present
Number research carries out the improvement of saline-alkali soil using Salt-resistant alkali-resistant microbial manure, and the wherein application of phosphate solubilizing microorganism fertilizer is research heat
Point.Insoluble phosphate-solubilizing can be water-soluble phosphorus by the phosphate solubilization of microorganism, effectively increase the content of soil quick-effective phosphor, production
Raw organic acid and active enzyme, promote the growth of crop.Identical measure is different to the improved effect of salt affected soil, comprehensive anti-
Good result could be obtained by controlling.
Existing saline-alkali soil modifying agent has very much, e.g., Chinese patent literature CN105670642A (application numbers
201610126908.6) a kind of saline-alkali soil conditioner and preparation method thereof is disclosed, it is main to include in parts by weight as follows
Raw material is made：500~600 parts of molasses alcohol waste liquid, 50~70 parts of mushroom bacteria residue, 45~60 parts of Irish moss, wormcast 30~40
Part, 18~20 parts of deer natural pond soil, 3~5 parts of heulandite, 3~5 parts of illite, 6~8 parts of sepiolite, 1 part of bacillus, acetic acid bacteria
0.3 part of 0.5 part, 0.1 part of lactic acid bacteria and yeast；Bacillus is by bacillus subtilis, bacillus licheniformis and huge gemma bar
Bacterium is using weight ratio as 2:1:1 composition, acetic acid bacteria is by acetobacter xylinum, acetobacter aceti and Acetobacter pasteurianus using weight ratio as 1:3:
2 compositions, lactic acid bacteria is by lactobacillus bulgaricus and lactobacillus acidophilus using weight ratio as 2:1 composition, yeast is by saccharomyces cerevisiae and grain
Wine fission yeast is using weight ratio as 2:1 composition.
It is special multiple that Chinese patent literature CN104609992A (application number 201410844584.0) discloses a kind of salt-soda soil
Close the raw material composition of bacterial manure and preparation method thereof, the composite bacterial fertilizer, including following percentage by weight：Dissolving phosphor and dissolving potassium class compound bacteria
Agent 10~20%, kitchen garbage 40~60%, diatomite 30~40%.Wherein dissolving phosphor and dissolving potassium class composite bacteria agent is by huge gemma
Bacillus, bacillus thuringiensis, Pseudomonas fluorescens, bacillus amyloliquefaciens, colloid bacillus cereus, azotobacter chroococcum are trained respectively
Mixed in equal amounts obtains after supporting centrifugation, freeze-drying；Kitchen garbage is by kitchen garbage pulverizing that is sorted, cleaning and be dehydrated oil removing
Afterwards, composite bacteria agent and mixing enzyme preparation are added, is obtained after aerobic solid-state fermentation.
Chinese patent literature CN104230600A (application number 201410463730.5) discloses a kind of saline-alkali soil amemdment
And preparation method thereof, it is made of following parts by weight：30~70 parts of enzyme preparation filter residues, 10~60 parts of bone meal, 1~5 portion of microbial inoculum；It is described
Microbial inoculum is bacillus subtilis bacterium powder, the one or more combination in jelly bacillus bacterium powder, bacillus megaterium bacterium powder.Should
Invention adsorbs microorganism formulation with enzyme preparation filter residue, and compounds rich in biological phosphorus, the bone meal of calcium, and enzyme preparation filter residue is to crop cause of disease
Bacterium has good inhibiting effect, effectively prevents the generation of soil-borne disease, while promotes beneficial bacteria such as nitrogen-fixing bacteria, fiber hydrolization
Bacterium, lactic acid bacteria, the hyperplasia of actinomyces, suppress the growth of harmful bacteria such as mould, der Pilz；Bone meal adjust soil neutral and alkali from
Son, improves soil microenvironment using the mixed microorganism such as dissolving phosphor and dissolving potassium and bacillus subtilis preparation.
Chinese patent literature CN106588424A (application number 201610790537.1) discloses a kind of salt affected soil improvement
It is slow-release fertilizer dedicated, it is characterized in that, it is by 25~35wt% of medical stone, 5~10wt% of potassium chloride, 5~15wt% of mineral powder
With 25~30wt% of organic fertilizer, water is prepared, and the sum of each component is 100%, and active solid strain includes lactic acid bacteria, withered grass bud
Spore bacillus, bacillus megaterium, trichoderma, aspergillus niger, monascus, trichoderma harzianum, microorganism Aspergillus aculeatus and yeast, mineral powder are grass
One or more kinds of mixtures in charcoal, zeolite, vermiculite, montmorillonite, calcium carbonate, expanded perlite, mineral powder it is thin
≤ 200 mesh are spent, the organic fertilizer contains decomposed muck, rapeseed cake fertilizer, stalk, the weight of the muck, rapeseed cake fertilizer and stalk
Amount is than being 5.5~7.5: 2.5~3.5: 0.5~1.5.
Chinese patent literature CN102408264A (application 201110246079.2) discloses one kind and utilizes waste phosphorus tailing
Organic fertilizer prepared by powder and preparation method thereof, the parts by weight of raw materials of the organic fertilizer is as follows：20~35 parts of phosphorite tailings powder, agriculture
20~40 parts of crop material powder, 20~40 parts of soy sauce residues.By phosphorite tailings powder, agricultural residues powder and soy sauce residues are by weight proportion
It is uniformly mixed, by material-water ratio 1:2~1:3, add water, the aspergillus niger (Aspergillus niger) of inoculation 4~5%, 2~3%
Acetic acid bacteria (Acetobacter), 3~4% lactic acid bacteria (Lactobacillus), 4~6% bacillus megaterium
(Bacillus megaterium), ferments 3~5 days at 28~32 DEG C；Then, add water 15~20%, issued then at 50~65 DEG C
Ferment 10~15 days；Drying, packaging.
Chinese patent literature CN105925273A (application number 201610372129.4) discloses one kind and contains Lotus Leafextract
Soil conditioner method that original soil greening is carried out to drought and waterlogging salt-soda soil, soil conditioner of the present invention includes following parts by weight
Component：22~24 parts of fermentation ostrich excrement, 8~10 parts of peanut shell powder, 5~7 parts of biogas residue, 2~4 parts of ground phosphate rock, desulfuration waste 2
~4 parts, 1~3 part of ardealite, 2~4 parts of ferrous sulfate, 2~4 parts of potassium dihydrogen phosphate, 2~4 parts of disodium hydrogen phosphate, amino-acid salt 1
~3 parts, 0.5~0.7 part of malonic acid, 0.1~0.3 part of thermophilic salt methane backeria, 0.1~0.3 part of bacillus subtilis, huge gemma bar
0.1~0.3 part of bacterium, 0.1~0.3 part of bacillus licheniformis, 0.1~0.3 part of saccharomycete, 0.2~0.4 part of atrazine, glyphosine
0.2~0.4 part, adjust 0.2~0.4 part of phosphine, 0.2~0.4 part of Lotus Leafextract, 1~3 part of pectin, 1~3 part of alginic acid, chitosan
1~3 part, 1~3 part of heparin, 2~4 parts of tricalcium phosphate, 0.3~0.5 part of cellulose graft acrylate polymer, chelated iron 0.1
~0.3 part, 0.1~0.3 part of chelated zinc.
The microbial bacterial agent of existing improvement saline-alkali soil is made of a variety of different strains above, and be bound to producing strains between strain
Group's competition and the effect of antagonism, repair to saline-alkali soil because a variety of different strains in the influence of varying environment and region and
The effect of generation is unstable.
Chinese patent literature CN105154357A (application number 201510505726.5) discloses a kind of secondary salinization soil
The preparation method and application of earth remediation microbial inoculum, the secondary salinization soil remediation microbial inoculum contain the bacillus megaterium bacterium after freezing
Strain zymotic fluid and protective agent, the bacillus megaterium are specially：Bacillus megaterium NCT-2, in being preserved in
State's Microbiological Culture Collection administration committee common micro-organisms center, deposit number are CGMCC No.4698；The protective agent
Using sucrose, mannitol, Twen 80, glycerine, skimmed milk power, sorbierite, starch, L-sodium, maltodextrin, glucose,
Any one or the combination of several of them in trehalose；Wherein, bacillus megaterium can not only reduce the content of soil nitrate-N
With EC values, and the content of soil with organic matter can be increased, improve vegetable yields and quality.
Biological modification is combined with chemical modifying in the present invention, by resistance to high salt, soda acid, phosphate solubilizing microorganism and salt can be alleviated
Material and organic-inorganic the nutrient compounding of stress, obtain the microbial bacterial agent with improvement saline-alkali soil.
The content of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of reclamation of salinep-alkali soil type microbial bacterial agent and preparation method thereof,
The saline and alkaline phenomenon of soil can be alleviated, soil is significantly improved, be effectively improved crop quality, reach water conservation, fertilizer conservation, improve soil matter
Amount and crop yield.
The technical solution adopted by the present invention is：
A kind of reclamation of salinep-alkali soil type microbial bacterial agent, component is as follows, is parts by weight：
10~30 parts of bacillus megaterium HB bacterium powders, 35~65 parts of phosphorus mine tailing, 12~18 parts of ardealite, sugar refinery waste residue 8
~25 parts, 6~26 parts of seaweed slag, 1~8 part of mannitol；
The bacillus megaterium HB bacterium powders contain gemma 5.0 × 109~1.2 × 1010A/g；
The microbial bacterial agent contains living bacteria count 1.0 × 109~3.0 × 109CFU/g。
Sugar refinery waste residue of the present invention is sugarcane sugar residue, is ordinary commercial products.
Seaweed slag main component of the present invention is as follows, by percentage to the quality：Crude protein 15-20%, crude fibre
40-50%, alginic acid 5-8%, surplus are inevitable impurity；It is to produce alginic acid by sargassum, potassium carbonate raw material and obtain
The accessory substance arrived, ordinary commercial products.
Bacillus megaterium (Bacillus megaterium) HB, is preserved in China Microbiological on January 7th, 2016
Culture presevation administration committee common micro-organisms center, address：The Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese Academy of Sciences
Institute of microbiology, deposit number are：CGMCC NO.11961；Bacillus megaterium (Bacillus megaterium) HB is
Gram-positive bacteria and aerobic bacteria, rod-shaped, end circle is single or arranged in short chain, 1.2~1.5 × 2.0~4.0 microns, can transport
It is dynamic；1.0~1.2 × 1.5~2.0 microns of gemma, ellipse, middle life or the life of secondary end.
A kind of preparation method of reclamation of salinep-alkali soil type microbial bacterial agent, including step are as follows：
(1) prepared by function bacterium bacillus megaterium seed liquor
A, bacillus megaterium bacterial strain activates；
B, by the bacillus megaterium inoculation of activation into liquid seed culture medium, at 32~37 DEG C cultivate 16~
24h, obtains bacillus megaterium seed liquor, spare；
(2) preparation of pre-treated mixed material
In proportion, bean cake powder, straw powder, wheat bran, vinasse, corn flour is taken to be uniformly mixed, bean cake powder, straw powder, wheat bran, wine
Grain, the parts by weight of corn flour are as follows：40~70 parts of bean cake powder, 10~28 parts of straw powder, 10~22 parts of wheat bran, vinasse 10~35
Part, 5~15 parts of corn flour；Add water to adjust moisture content to 60~70wt%, in stood under the conditions of 20~35 DEG C 8~24 it is small when, so
After 20~40min of boiling at 115~121 DEG C of temperature, after cooling pre-treated mixed material, it is spare；
(3) pre-treated mixed material made from step (2) is taken, accesses step by mass percentage for the inoculum concentration of 8~20wt%
Suddenly the bacillus megaterium seed liquor that prepared by (1), is uniformly mixed, and when placement 24~30 is small under the conditions of 32~36 DEG C, then turns over
Expect, stir evenly, then improve temperature to 38~42 DEG C, continue fermentation 24~48 it is small when, cool the temperature to room temperature after fermentation,
Obtain fermentate；
(4) fermentate in step (3) is dried to biodiversity content below 15% at 50~110 DEG C, at crushing
Bacillus megaterium HB bacterium powders are obtained after reason, contain gemma number 5.0 × 109~1.2 × 1010A/g, spore production rate reach 90% with
On；
(5) after phosphorus mine tailing, ardealite, sugar refinery waste residue, seaweed slag crushed 5~20 mesh sieves, obtained with step (4)
Bacillus megaterium HB bacterium powders, mannitol are compounded in proportion, are stirred evenly, that is, obtain reclamation of salinep-alkali soil type microbial bacteria
Agent.
Preferable according to the present invention, the activation medium described in step (1) A is LB culture mediums, its component and weight hundred
Divide ratio as follows：Tryptone 0.8~1.2%, yeast extract are 0.4~0.6%, sodium chloride 0.4~0.6%, agar powder 1.5
~2.0%, remaining is water, pH value 7.2.
Preferable according to the present invention, in step (1) A, the activation is that the bacillus megaterium HB of Cord blood exists
Single bacterium colony is turned out on LB plating mediums under the conditions of 37 DEG C, picking single bacterium colony is cultivated on LB slant mediums under the conditions of 37 DEG C
18~24h.
Preferable according to the present invention, liquid seed culture medium component and its weight percent described in step (1) B are such as
Under：Glucose 2.0~3.0%, Dried Corn Steep Liquor Powder 1.1~1.8%, analysis for soybean powder 1.5~4.0%, yeast extract 0.1~0.2%,
Potassium dihydrogen phosphate 0.01~0.03%, sodium chloride 0.01~0.02%, magnesium sulfate 0.01~0.02%, remaining is water, and pH value is
7.0~7.3.
Preferable according to the present invention, the inoculum concentration of bacillus megaterium seed liquor is 10~19wt% in step (3), in 33
When placement 25~30 is small under the conditions of~35 DEG C.
Highly preferred, the inoculum concentration of bacillus megaterium seed liquor is 15wt% in step (3), is placed under the conditions of 34 DEG C
30 it is small when.
Do not limited in detail in above-mentioned preparation method according to the prior art.
Above-mentioned reclamation of salinep-alkali soil type microbial bacterial agent as phosphobacteria microbial inoculum, alternatively, as growth-promoting, increase soil fertility, improve it is saline and alkaline
The application of the microbial manure of soil.
Preferable according to the present invention, above application, amount of application is 30~80kg to reclamation of salinep-alkali soil type microbial bacterial agent per acre.
Beneficial effect：
1. microbial inoculum prepared by the present invention contains the gemma hypopus of the bacillus megaterium of a large amount of high activities, effective gemma number
Up to 1.0 × 109A/more than g, the bacterial strain gemma have resistance to 110 DEG C of high temperature, high salts, and colonization ability is strong, and reproduction speed is fast
Feature, can quickly colonize after being manured into soil in crop rhizosphere, improve rhizosphere soil micro-ecological environment, with keeping on top strain
Position, captures the living space of harmful bacteria.
2. producing the activation factors such as organic acid, phytase in the bacillus megaterium reproductive process in the present invention, can live
Change in soil by fixed element, invalid phosphorus is converted into available phosphorus, is effectively improved in the chemical fertilizer of application and in soil
The absorption efficiency of phosphorus, promotes collaboration of the crop to potassium, calcium, magnesium to absorb, can improve chemical fertilizer utilization rate more than 10%；Together
When, rhizosphere soil acid-base value can be buffered, mitigates salinization of soil degree, improves crop anti-adversity energy；Metabolism produces indoles second
Sour (IAA), heteroauxin is a kind of natural plant growth promoter, promotes seed sprouting, crop and root growth.
3. reclamation of salinep-alkali soil microbial bacterial agent combination salt-soda soil provided by the invention actual conditions, utilize biotechnology and day
Right mineral activation technology, recovers soil ecosystem, alleviates salinization of soil problem.The core of this technology be acid-base balance and
Soil micro-ecosystem balances, and using the synergistic effect of resistance to high salt, the microorganism of soda acid and natural mineral matter, dredges duct, raising pair
The absorption of mineral matter and salt ion and the ability of ion exchange, are conducive to salt elution, suppress the accumulation of salt in the surface soil, play and control the work that salt changes alkali
With, and colonize using the quick of functional microorganism, expand numerous and distinctive metabolite activating rhizosphere micro-ecological environment, promote soil
Earth microecological balance.
4. microbial inoculum preparation process of the present invention is simple, with short production cycle, safe, shelf life length, and realizes discarded
The recycling in goods and materials source, sugar refinery waste residue, seaweed slag in microbial inoculum are rich in organic nutrients such as abundant protein, vitamins, greatly
The yield and quality of crop is improved, it is synergistic with the phosphorus mine tailing in formula, ardealite, mannitol, lack any type
Component, all can not achieve the desired results.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, come below with specific embodiment into one
Step clearly explains the present invention, but is not construed as limiting the invention in any way.Experimental method in following embodiments,
Unless otherwise instructed, it is conventional method；Percentage composition in following embodiments, is that weight percent contains unless otherwise instructed
Amount.
Bacillus megaterium HB bacterial strains, it is general to be preserved in China Committee for Culture Collection of Microorganisms on January 7th, 2016
Logical microorganism center, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, deposit number
For CGMCC NO.11961.
Phosphorus mine tailing develops limited parent company purchased from Guizhou Wengfu industry, containing P2O58.2%th, calcium oxide 35%, magnesia
15.5%th, SiO24.4% and a small amount of iron；
Sugar refinery waste residue is sugarcane sugar residue, and purchased from Guangxi FuZhe trade Co., Ltds, main component is blackstrap, albumen, thick
Fat, crude fibre；
Seaweed slag is purchased from Kingenta Ecological Engineering Group Co., Ltd.；It is using sargassum as raw material, using potassium carbonate
Chemical degradation method production is carried out, main component is alginic acid, crude protein, crude fibre；
Common organic fertilizer (N-P-K >=5%) purchase is rich in Linyi state.
Ardealite, mannitol are ordinary commercial products, content more than 80%.
The present invention is described in further details with reference to embodiment：According to the method in national standard GB/T 2087-2006
Be detected, as a result for：Microbial bacterial agent contains living bacteria count 1.0 × 109~3.0 × 109CFU/g。
A kind of reclamation of salinep-alkali soil type microbial bacterial agent of embodiment 1 and preparation method thereof, step is as follows：
(1) prepared by bacillus megaterium seed liquor
A, the bacillus megaterium HB of Cord blood is cultivated into single bacterium colony on LB plating mediums under the conditions of 37 DEG C, picking
Single bacterium colony cultivates 18h on LB slant mediums under the conditions of 37 DEG C, obtains the bacterial strain of activation；
B, with the inoculation of sterile oese scraping one ring step A activation into 50ml liquid seed culture mediums,
35 DEG C of culture 20h, up to bacillus megaterium bacterium solution, bacterium solution viable count 1.5 × 109CFU/ml。
(2) preparation of pre-treated mixed material
By mass parts, 40 parts of bean cake powder is taken, 20 parts of straw powder, 20 parts of wheat bran, 15 parts of vinasse, 5 parts of corn flour, is put into container
It is interior to be uniformly mixed, add water to adjust to moisture 60wt%, when placement 16 is small under the conditions of 25 DEG C；Boiling under the conditions of 121 DEG C
30min, obtains mixture, and cooling is spare.
(3) by mass percentage, the pre-treated mixed material for taking step (2) to prepare, step is accessed by the inoculum concentration of 10wt%
(1) the bacillus megaterium seed liquor prepared, is uniformly mixed, and when placement 28 is small under the conditions of 36 DEG C, then stirring, stirring are equal
It is even, then improve temperature to 40 DEG C, continue fermentation 28 it is small when, cool the temperature to room temperature after fermentation, obtain fermentate.
(4) fermentate in step (3) is dried to biodiversity content below 15% under the conditions of 80 DEG C, at crushing
Bacillus megaterium bacterium powder is obtained after reason, contains gemma number 9.0 × 109A/g.
(5) after phosphorus mine tailing, ardealite, sugar refinery waste residue, seaweed slag crushed 5~20 mesh sieves, obtained with step (4)
Bacillus megaterium bacterium powder, mannitol are compounded, and specific ratio is as follows：
(6) above-mentioned each raw material is measured in batches by the technical recipe requirement of step (5), be delivered in batch mixer, Suo Youyuan
Material stirs evenly, and obtains a kind of reclamation of salinep-alkali soil type microbial bacterial agent powder product, living bacteria count 1.8 × 109CFU/g。
A kind of reclamation of salinep-alkali soil type microbial bacterial agent of embodiment 2 and preparation method thereof, step is as follows：
(1) prepared by bacillus megaterium seed liquor
A, the bacillus megaterium bacterial strain HB of Cord blood is turned out into single bacterium on LB plating mediums under the conditions of 37 DEG C
Fall, picking single bacterium colony cultivates 24h on LB slant mediums under the conditions of 37 DEG C, obtains the bacterial strain of activation；
B, with the inoculation of sterile oese scraping one ring step A activation into 50ml liquid seed culture mediums,
36 DEG C of culture 22h, up to bacillus megaterium seed liquor, bacterium solution viable count 2.1 × 109CFU/ml。
(2) preparation of pre-treated mixed material
By mass parts, 45 parts of bean cake powder is taken, 20 parts of straw powder, 10 parts of wheat bran, 15 parts of vinasse, 10 parts of corn flour, is put into appearance
It is uniformly mixed in device, adds water to adjust to moisture 70wt%, when placement 24 is small under the conditions of 30 DEG C；Boiling under the conditions of 120 DEG C
40min, obtains mixture, and cooling is spare.
(3) by mass percentage, the pre-treated mixed material for taking step (2) to prepare, step is accessed by the inoculum concentration of 15wt%
(1) the bacillus megaterium seed liquor prepared, is uniformly mixed, and when placement 30 is small under the conditions of 36 DEG C, then stirring, stirring are equal
It is even, then improve temperature to 42 DEG C, continue fermentation 36 it is small when, cool the temperature to room temperature after fermentation, obtain fermentate.
(4) fermentate in step (3) is dried to biodiversity content below 15% under the conditions of 100 DEG C, through crushing
Bacillus megaterium bacterium powder is obtained after processing, contains gemma number 1.1 × 1010A/g.
(5) after phosphorus mine tailing, ardealite, sugar refinery waste residue, seaweed slag crushed 5~20 mesh sieves, obtained with step (4)
Bacillus megaterium bacterium powder, mannitol are compounded, and specific ratio is as follows：
(6) above-mentioned each raw material is measured in batches by the technical recipe requirement of step (5), be delivered in batch mixer, Suo Youyuan
Material stirs evenly, and obtains a kind of reclamation of salinep-alkali soil type microbial bacterial agent powder product, living bacteria count 1.1 × 109CFU/g。
A kind of reclamation of salinep-alkali soil type microbial bacterial agent of embodiment 3 and preparation method thereof, step is as follows：
(1) prepared by bacillus megaterium seed liquor
A, the bacillus megaterium bacterial strain HB of Cord blood is turned out into single bacterium on LB plating mediums under the conditions of 37 DEG C
Fall, picking single bacterium colony cultivates 24h on LB slant mediums under the conditions of 37 DEG C, obtains the bacterial strain of activation；
B, with the inoculation of sterile oese scraping one ring step A activation into 50ml liquid seed culture mediums,
37 DEG C of culture 24h, up to bacillus megaterium bacterium solution, bacterium solution viable count 1.1 × 109CFU/ml。
(2) preparation of pre-treated mixed material
By mass parts, 65 parts of bean cake powder is taken, 25 parts of straw powder, 20 parts of wheat bran, 35 parts of vinasse, 15 parts of corn flour, is put into appearance
It is uniformly mixed in device, adds water to adjust to moisture 70wt%, when placement 24 is small under the conditions of 35 DEG C；Boiling under the conditions of 120 DEG C
40min, obtains mixture, and cooling is spare.
(3) by mass percentage, the pre-treated mixed material for taking step (2) to prepare, step is accessed by the inoculum concentration of 20wt%
(1) the bacillus megaterium seed liquor prepared, is uniformly mixed, and when placement 30 is small under the conditions of 36 DEG C, then stirring, stirring are equal
It is even, then improve temperature to 42 DEG C, continue fermentation 36 it is small when, cool the temperature to room temperature after fermentation, obtain fermentate.
(4) fermentate in step (3) is dried to biodiversity content below 15% under the conditions of 100 DEG C, through crushing
Bacillus megaterium bacterium powder is obtained after processing, contains gemma number 1.11 × 1010CFU/g。
(5) after phosphorus mine tailing, ardealite, sugar refinery waste residue, seaweed slag crushed 5~20 mesh sieves, obtained with step (4)
Bacillus megaterium bacterium powder, mannitol are compounded, and specific ratio is as follows：
(6) above-mentioned each raw material is measured in batches by the technical recipe requirement of step (5), be delivered in batch mixer, Suo Youyuan
Material stirs evenly, and obtains a kind of reclamation of salinep-alkali soil type microbial bacterial agent powder product, living bacteria count 1.11 × 109CFU/g。
A kind of reclamation of salinep-alkali soil type microbial bacterial agent of embodiment 4 and preparation method thereof, step is as follows：
(1) prepared by bacillus megaterium seed liquor
A, the bacillus megaterium bacterial strain HB of Cord blood is turned out into single bacterium on LB plating mediums under the conditions of 37 DEG C
Fall, picking single bacterium colony cultivates 18h on LB slant mediums under the conditions of 37 DEG C, obtains the bacterial strain of activation；
B, with the inoculation of sterile oese scraping one ring step A activation into 50ml liquid seed culture mediums,
32 DEG C of culture 16h, up to bacillus megaterium bacterium solution, bacterium solution viable count 1.2 × 109CFU/ml。
(2) preparation of pre-treated mixed material
By mass parts, 41 parts of bean cake powder is taken, 15 parts of straw powder, 10 parts of wheat bran, 15 parts of vinasse, 7 parts of corn flour, is put into container
It is interior to be uniformly mixed, add water to adjust to moisture 65wt%, when placement 20 is small under the conditions of 30 DEG C；Boiling under the conditions of 120 DEG C
40min, obtains mixture, and cooling is spare.
(3) by mass percentage, the pre-treated mixed material for taking step (2) to prepare, step is accessed by the inoculum concentration of 15wt%
(1) the bacillus megaterium seed liquor prepared, is uniformly mixed, and when placement 30 is small under the conditions of 36 DEG C, then stirring, stirring are equal
It is even, then improve temperature to 42 DEG C, continue fermentation 36 it is small when, cool the temperature to room temperature after fermentation, obtain fermentate.
(4) fermentate in step (3) is dried to biodiversity content below 15% under the conditions of 100 DEG C, through crushing
Bacillus megaterium bacterium powder is obtained after processing, contains gemma number 1.29 × 1010CFU/g。
(5) after phosphorus mine tailing, ardealite, sugar refinery waste residue, seaweed slag crushed 5~20 mesh sieves, obtained with step (4)
Bacillus megaterium bacterium powder, mannitol are compounded, and specific ratio is as follows：
(6) above-mentioned each raw material is measured in batches by the technical recipe requirement of step (5), be delivered in batch mixer, Suo Youyuan
Material stirs evenly, and obtains a kind of reclamation of salinep-alkali soil type microbial bacterial agent powder product, living bacteria count 1.29 × 109CFU/g。
Comparative example 1
Preparation process is with embodiment 1, except that addition bacterium numbering is huge for CGMCC NO.4698 in step (5)
Bacillus bacterium powder.
Comparative example 2
Preparation process is with embodiment 1, except that not adding phosphorus mine tailing in step (5).
Comparative example 3
Preparation process is with embodiment 1, except that addition alditol slag substitutes sugar refinery waste residue in step (5).
Comparative example 4
Preparation process is with embodiment 1, except that addition manioc waste substitutes seaweed slag in step (5).
Comparative example 5
Preparation process is with embodiment 1, except that not adding ardealite in step (5).
A kind of field experiment of reclamation of salinep-alkali soil type microbial bacterial agent of test example 1
Selection soil is In Wulumuqi salt affected soil, soil pH 7.9, organic matter 0.9%, hydrolyzable nitrogen
159.6mg/kg, rapid available phosphorus 24.5mg/kg, available potassium 150.9mg/kg.Experimental field gross area 720m2, long 60m, wide 12m, altogether
35 furrow, plantation color green pepper kind is yellow star 1, per 56 plants, transplanting plant spacing 50cm, line-spacing 50cm of furrow.The fertilizer used is embodiment
1-4 prepare reclamation of salinep-alkali soil type microbial bacterial agent, comparative example 1-5 prepare fertilizer, the common organic fertilizer of equivalent (Linyi state is rich,
N-P-K >=5%) and clear water control, fertilizer application mode is base manure, 30kg/ mus.Each experiment plot seeding method, spacing in the rows, OK
It is consistent away from, management measure etc., calculates whole growth cycle harvesting yield, and take rhizosphere soil to carry out n-p-k element point
Analysis.Other fertilizer are not applied in all processing.
1 color green pepper of table applies reclamation of salinep-alkali soil type microbial bacterial agent and applies influence of the common organic fertilizer to part index number
The results show：Compared using the experiment plot of the embodiment 1-4 reclamation of salinep-alkali soil type microbial bacterial agent groups prepared
Ratio and control group volume increase are obvious, and the microbial inoculum that wherein prepared by the highest embodiment 1 of yield is compared with clear water control, the control of common organic fertilizer
Volume increase 19.4%, 12.88%, Vc contents are respectively increased 28.3%, 19.2% respectively, compared with the highest comparative example of yield in comparative example
5th, the minimum comparative example 1 of yield increases production 7.01%, 8.6%, Vc contents and is respectively increased 12.6%, 16.2% respectively；Yield is minimum
Embodiment 2 prepare microbial inoculum compared with clear water control, common organic fertilizer control respectively volume increase 16.97%, 10.6%, Vc contents difference
22.8%, 14.1% is improved, the comparative example 1 minimum compared with the highest comparative example 5 of yield in comparative example, yield is increased production respectively
7.54%th, 9.40%, Vc contents are respectively increased 8.8%, 12.5%.
Reclamation of salinep-alkali soil type microbial inoculum group color green pepper rhizosphere soil pH in embodiment is compared with clear water blank, common organic fertilizer treatment group
More than 0.43 is reduced, more than 0.32 is reduced compared with comparative example 1-5.Reach using the salt-soda soil nitrogen use efficiency of microbial bacterial agent
49.9%, 16.8 percentage points are improved than common organic fertilizer group, more than 11.1 percentage points are improved compared with comparative example 1-5；Phosphorus utilizes
Rate reaches 39.5%, improves 11.2 percentage points than common organic fertilizer group, potassium utilization rate reaches 72.4%, than common organic fertilizer
Group improves 10.2 percentage points, and more than 7.7,8.5 percentage points are respectively increased compared with comparative example 1-5 phosphorus, potassium utilization rate.
Comparative example 1-5 lacks component in microbial inoculum formula, can not play synergistic function.Fully demonstrate the salt of the present invention
Alkali soil improvement type microbial bacterial agent has the nitrogen that increases significantly, phosphorus, potassium utilization rate, reduction crop rhizosphere soils pH, raising fruit
Vc contents, growth-promoting, the effect of volume increase, and component is indispensable in microbial inoculum formula.As seen from the above table, using reclamation of salinep-alkali soil type
Microbial bacterial agent relatively apply common organic fertilizer group be averaged mu volume increase 768.25kg, yield increase by 11.8%.
A kind of field experiment of reclamation of salinep-alkali soil type microbial bacterial agent of test example 2
Selection soil is calacareous soil, meta-alkalescence, soil pH 8.1, organic matter 1.1%, hydrolyzable nitrogen 152.6mg/
Kg, rapid available phosphorus 14.5mg/kg, available potassium 150.2mg/kg.8 meters of trial zone row length, 10 row areas, line-spacing 15cm.Plant wheat product
Kind is Jimai 22, and application rate is：118kg/hm2.The fertilizer used is reclamation of salinep-alkali soil type microbial bacteria prepared by embodiment 1,2
Fertilizer, the common organic fertilizer of equivalent (Linyi state is rich, N-P-K >=5%) and clear water control prepared by agent, comparative example 1-5, fertilizer application
Mode 50% makees base manure, 50% jointing top dressing.Seeding method, method of comparison arrangement, repeats three times.Calculate harvest each cell seed and
Straw biological yield, takes seed to calculate seed stem ratio, and takes rhizosphere soil to carry out n-p-k element analysis.Field management is according to high yield
It is required that and biophysiography character.Other fertilizer are not applied in all processing.
Influence of the reclamation of salinep-alkali soil type microbial bacterial agent to wheat yield of 2 winter wheat of table
| Project | Yield (kg/ mus) | Fringe grain (grain) | Mass of 1000 kernel (g) | Nitrogen use efficiency (%) | Phosphorus utilization (%) | Potassium utilization rate (%) |
| 1 microbial inoculum of embodiment | 569.9 | 30.8 | 40.9 | 40.5 | 30.9 | 68.1 |
| 2 microbial inoculum of embodiment | 568.7 | 30.5 | 40.8 | 40.1 | 30.9 | 67.1 |
| 3 microbial inoculum of embodiment | 569.6 | 30.8 | 41.1 | 40.3 | 31.2 | 68.1 |
| 4 microbial inoculum of embodiment | 560.9 | 30.2 | 40.9 | 40.3 | 30.1 | 66.9 |
| Comparative example 1 | 508.8 | 25.3 | 35.4 | 32.8 | 18.4 | 59.8 |
| Comparative example 2 | 521.1 | 27.8 | 37.1 | 34.6 | 19.5 | 60.7 |
| Comparative example 3 | 514.9 | 26.7 | 36.2 | 34.2 | 19.7 | 61.2 |
| Comparative example 4 | 519.1 | 27.3 | 36.6 | 33.9 | 19.4 | 61.8 |
| Comparative example 5 | 521.5 | 28.3 | 37.1 | 33.5 | 19.7 | 60.9 |
| Common organic fertilizer | 495.6 | 26.6 | 35.5 | 32.1 | 18.3 | 59.2 |
| Clear water | 353.5 | 18.9 | 28.1 | —— | —— | —— |
The results show：In calacareous soil, the reclamation of salinep-alkali soil type microbial bacterial agent group prepared using embodiment 1-4 is equal
There is volume increase by a relatively large margin than control group, comparative example 1-5, the wherein highest embodiment 1 of yield is minimum compared with yield in comparative example
The volume increase of comparative example 1 12%, compared with the highest volume increase of comparative example 5 9.3% of yield in comparative example；Reclamation of salinep-alkali soil type microbial bacterial agent exists
Nitrogen use efficiency reaches 40.5% in Wheat Production, and 8.4 percentage points are improved than common organic fertilizer group, and 5.9 are improved compared with comparative example 1-5
More than percentage point；Phosphorus utilization reaches 31.2%, 12.9 percentage points is improved than common organic fertilizer group, compared with comparative example 1-5
Improve more than 11.5 percentage points；Potassium utilization rate reaches 68.1%, and 8.9 percentage points are improved than common organic fertilizer group, more right
Ratio 1-5 improves more than 6.3 percentage points.
Comparative example 1-5 lacks component in microbial inoculum formula, can not play synergistic function, fully demonstrates the salt of the present invention
Alkali soil improvement type microbial bacterial agent has the effect of increase significantly nitrogen, phosphorus, potassium utilization rate, and component lacks one in microbial inoculum formula
Can not.As seen from the above table, relatively apply common organic fertilizer group mu using reclamation of salinep-alkali soil type microbial bacterial agent and increase production 71.7kg, yield increases
Add 14.5%.
Experimental teaching of agronomy the result shows that, each component is synergistic in reclamation of salinep-alkali soil type microbial bacteria agent prescription of the present invention, lacks
Few any type, all falls flat, and only uses the situation of reclamation of salinep-alkali soil type microbial bacterial agent full formula of the present invention
Under, it can be played under conditions of 30~80 kilograms of usage amount per acre and improve utilization rate of fertilizer, crop yield, raising product well
Matter, the effect for improveing salt-soda soil.Reclamation of salinep-alkali soil type microbial bacterial agent of the present invention can be used as base manure, top dressing etc. to use, microbial inoculum
Usage amount need to be determined according to factors such as soil regime, crop varieties, Crop growing stage, crop nutrition conditions.
Claims (10)
1. a kind of reclamation of salinep-alkali soil type microbial bacterial agent, component is as follows, is parts by weight：
10~30 parts of bacillus megaterium HB bacterium powders, 35~65 parts of phosphorus mine tailing, 12~18 parts of ardealite, sugar refinery waste residue 8~25
Part, 6~26 parts of seaweed slag, 1~8 part of mannitol；
The bacillus megaterium HB bacterium powders contain gemma 5.0 × 109~1.2 × 1010A/g；
The microbial bacterial agent contains living bacteria count 1.0 × 109~3.0 × 109CFU/g。
2. microbial bacterial agent as claimed in claim 1, it is characterised in that the bacillus megaterium (Bacillus
Megaterium) HB, on January 7th, 2016 are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, ground
Location：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, deposit number are：CGMCC
NO.11961。
3. a kind of preparation method of reclamation of salinep-alkali soil type microbial bacterial agent, including step are as follows：
(1) prepared by function bacterium bacillus megaterium seed liquor
A, bacillus megaterium bacterial strain activates；
B, by the bacillus megaterium inoculation of activation into liquid seed culture medium, 16~24h is cultivated at 32~37 DEG C,
Bacillus megaterium seed liquor is obtained, it is spare；
(2) preparation of pre-treated mixed material
In proportion, take bean cake powder, straw powder, wheat bran, vinasse, corn flour to be uniformly mixed, bean cake powder, straw powder, wheat bran, vinasse,
The parts by weight of corn flour are as follows：40~70 parts of bean cake powder, 10~28 parts of straw powder, 10~22 parts of wheat bran, 10~35 parts of vinasse are beautiful
5~15 parts of rice flour；Water is added to adjust moisture content to 60~70wt%, when standing 8~24 is small under the conditions of 20~35 DEG C, then in temperature
20~40min of boiling at 115~121 DEG C of degree, obtains pre-treated mixed material after cooling, spare；
(3) pre-treated mixed material made from step (2) is taken, accesses step (1) by mass percentage for the inoculum concentration of 8~20wt%
The bacillus megaterium seed liquor of preparation, be uniformly mixed, under the conditions of 32~36 DEG C place 24~30 it is small when, then stirring, stir
Mix uniformly, then improve temperature to 38~42 DEG C, continue fermentation 24~48 it is small when, cool the temperature to room temperature after fermentation, obtain
Fermentate；
(4) fermentate in step (3) is dried to biodiversity content below 15% at 50~110 DEG C, after pulverization process
Bacillus megaterium HB bacterium powders are obtained, contain gemma number 5.0 × 109~1.2 × 1010A/g, spore production rate reach more than 90%；
(5) after phosphorus mine tailing, ardealite, sugar refinery waste residue, seaweed slag crushed 5~20 mesh sieves, obtained with step (4) huge
Bacillus HB bacterium powders, mannitol are compounded in proportion, are stirred evenly, that is, obtain reclamation of salinep-alkali soil type microbial bacterial agent.
4. preparation method as claimed in claim 3, it is characterised in that the activation medium described in step (1) A is cultivated for LB
Base, its component and percentage by weight are as follows：Tryptone 0.8~1.2%, yeast extract are 0.4~0.6%, sodium chloride 0.4
~0.6%, agar powder 1.5~2.0%, remaining is water, pH value 7.2.
5. preparation method as claimed in claim 3, it is characterised in that in step (1) A, the activation is by Cord blood
Bacillus megaterium HB single bacterium colony is turned out under the conditions of 37 DEG C on LB plating mediums, picking single bacterium colony is in LB inclined-plane cultures
18~24h is cultivated on base under the conditions of 37 DEG C.
6. preparation method as claimed in claim 3, it is characterised in that the liquid seed culture medium component described in step (1) B
And its percentage by weight is as follows：Glucose 2.0~3.0%, Dried Corn Steep Liquor Powder 1.1~1.8%, analysis for soybean powder 1.5~4.0%, ferment
Female medicinal extract 0.1~0.2%, potassium dihydrogen phosphate 0.01~0.03%, sodium chloride 0.01~0.02%, magnesium sulfate 0.01~
0.02%, remaining is water, and pH value is 7.0~7.3.
7. preparation method as claimed in claim 3, it is characterised in that the inoculation of bacillus megaterium seed liquor in step (3)
Measure as 10~19wt%, in placed under the conditions of 33~35 DEG C 25~30 it is small when.
8. preparation method as claimed in claim 3, it is characterised in that the inoculation of bacillus megaterium seed liquor in step (3)
Measure as 15wt%, placed under the conditions of 34 DEG C 30 it is small when.
9. reclamation of salinep-alkali soil type microbial bacterial agent as claimed in claim 1 is as phosphobacteria microbial inoculum, alternatively, as growth-promoting, supporting
Ground, improve saline-alkali soil microbial manure application.
10. amount of application is 30~80kg to reclamation of salinep-alkali soil type microbial bacterial agent as claimed in claim 1 per acre.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5381375A (en) * | 1976-12-25 | 1978-07-18 | Rei Tech Inc | Method for ploughing straws into paddy fields or uplands |
| CN102408282A (en) * | 2011-08-15 | 2012-04-11 | 上海理工大学 | Organic seaweed fertilizer and application thereof as efficient saline-alkali soil improver |
| CN104261988A (en) * | 2014-09-26 | 2015-01-07 | 华南农业大学 | Special suspension liquid compound fertilizer for saline-alkali soil and preparation method of special suspension liquid compound fertilizer |
| CN105439726A (en) * | 2015-12-19 | 2016-03-30 | 佛山市艳晖生物科技有限公司 | Bacillus megaterium bacterial fertilizer for onsite fermentation in farm and applications thereof |
| CN106365709A (en) * | 2016-08-29 | 2017-02-01 | 五洲丰农业科技有限公司 | Preparation method of seaweed organic-inorganic fertilizer containing bacillus megatherium fungicide |
| CN106399157A (en) * | 2016-08-30 | 2017-02-15 | 上海绿乐生物科技有限公司 | Microbial agent with facility agricultural soil remediation function, and preparation and application of microbial agent |
-
2017
- 2017-12-05 CN CN201711271018.5A patent/CN108017445A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5381375A (en) * | 1976-12-25 | 1978-07-18 | Rei Tech Inc | Method for ploughing straws into paddy fields or uplands |
| CN102408282A (en) * | 2011-08-15 | 2012-04-11 | 上海理工大学 | Organic seaweed fertilizer and application thereof as efficient saline-alkali soil improver |
| CN104261988A (en) * | 2014-09-26 | 2015-01-07 | 华南农业大学 | Special suspension liquid compound fertilizer for saline-alkali soil and preparation method of special suspension liquid compound fertilizer |
| CN105439726A (en) * | 2015-12-19 | 2016-03-30 | 佛山市艳晖生物科技有限公司 | Bacillus megaterium bacterial fertilizer for onsite fermentation in farm and applications thereof |
| CN106365709A (en) * | 2016-08-29 | 2017-02-01 | 五洲丰农业科技有限公司 | Preparation method of seaweed organic-inorganic fertilizer containing bacillus megatherium fungicide |
| CN106399157A (en) * | 2016-08-30 | 2017-02-15 | 上海绿乐生物科技有限公司 | Microbial agent with facility agricultural soil remediation function, and preparation and application of microbial agent |
Non-Patent Citations (5)
| Title |
|---|
| 《彩图科技百科全书》编辑部: "《污染和灾害》", 31 January 2014, 上海科学技术出版社 * |
| 刘秀玲: "《农作物配方施肥新技术》", 28 February 2017, 河北科学技术出版社 * |
| 徐国方等: "《白对虾养殖》", 30 April 2015, 上海财经大学出版社 * |
| 许加超: "《海藻化学与工艺学》", 30 September 2014, 中国海洋大学出版社 * |
| 陈声明等: "《有机农业及其源头产品》", 30 June 2004, 中国环境科学出版社 * |
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Application publication date: 20180511 |