CN107695046B - Needle washing method based on multiple needle washing structures of sample adding needle - Google Patents
Needle washing method based on multiple needle washing structures of sample adding needle Download PDFInfo
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- CN107695046B CN107695046B CN201710752586.0A CN201710752586A CN107695046B CN 107695046 B CN107695046 B CN 107695046B CN 201710752586 A CN201710752586 A CN 201710752586A CN 107695046 B CN107695046 B CN 107695046B
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- 238000005406 washing Methods 0.000 title claims abstract description 285
- 238000000034 method Methods 0.000 title claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 154
- 238000011010 flushing procedure Methods 0.000 claims abstract description 56
- 239000002699 waste material Substances 0.000 claims abstract description 6
- 238000004140 cleaning Methods 0.000 claims description 26
- 238000002156 mixing Methods 0.000 claims description 10
- 238000005070 sampling Methods 0.000 claims description 6
- 238000011049 filling Methods 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 239000006210 lotion Substances 0.000 abstract description 12
- 206010011409 Cross infection Diseases 0.000 abstract description 6
- 206010029803 Nosocomial infection Diseases 0.000 abstract description 6
- 101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 description 20
- 239000000243 solution Substances 0.000 description 12
- 238000001514 detection method Methods 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000000427 antigen Substances 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000011068 loading method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000003749 cleanliness Effects 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
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- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/023—Cleaning the external surfaces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/027—Cleaning the internal surfaces; Removal of blockages
- B08B9/032—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing
- B08B9/0321—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing using pressurised, pulsating or purging fluid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B2209/00—Details of machines or methods for cleaning hollow articles
- B08B2209/02—Details of apparatuses or methods for cleaning pipes or tubes
- B08B2209/027—Details of apparatuses or methods for cleaning pipes or tubes for cleaning the internal surfaces
- B08B2209/032—Details of apparatuses or methods for cleaning pipes or tubes for cleaning the internal surfaces by the mechanical action of a moving fluid
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Abstract
The invention discloses a needle washing method based on a multi-needle washing structure of a sample adding needle, which comprises the following steps: the needle washing cup is provided with a waste liquid outlet at the lower end, the sample adding needle is vertically arranged above the needle washing cup, the needle head end of the needle washing cup can extend into the needle washing cup, the needle washing cup also comprises at least two liquid washing bottles, and each liquid washing bottle is provided with at least one air pressure pump correspondingly; the side wall of the needle washing cup is provided with an external flushing liquid inlet communicated with the interior of the needle washing cup; each washing liquid bottle is communicated with the needle tail end of the sample adding needle through an inner flushing pipeline and is communicated with an outer flushing liquid inlet through an outer flushing pipeline. Through setting up a plurality of lotion bottles, then through same interior pipeline and the inside intercommunication of application of sample needle, through same outer pipeline and the inside intercommunication of washing needle cup to the realization is washed and is mixed the washing alone to application of sample needle inner wall and syringe needle end outer wall, and washs many times, improves the washing dynamics of application of sample needle greatly, avoids application of sample needle to bring cross infection into, guarantees follow-up testing result's precision and reliability.
Description
Technical Field
The invention belongs to the field of chemiluminescent immunoassay detection equipment, and particularly relates to a needle washing method based on a multiple needle washing structure of a sample adding needle.
Background
Chemiluminescence immunoassay is a means for detecting by utilizing the specific reaction of an antigen and an antibody, because the detection signal can be amplified and displayed by utilizing isotope, enzyme, chemiluminescence and the like, when an experiment is carried out, the antigen or the antibody is connected with certain enzyme to form an enzyme-labeled antigen or antibody, the enzyme-labeled antigen or antibody not only retains the immunocompetence but also retains the activity of the enzyme, then a luminescent substrate is added after washing, in the process, a reagent is usually added into a reaction cup through a sample adding needle, because the sample adding needle is repeatedly used for many times, in order to avoid the situation that the sample adding needle brings detection liquid in one reaction cup into the other reaction cup, the sample adding needle is usually cleaned after being added, in the prior art, the sample adding needle is basically only adopted in a most basically overflowing needle washing mode, the mode is difficult to completely clean the sample adding needle, and the problem of cross infection of the sample adding needle is easy to occur, disturbing the detection results, even making the results completely contradictory.
Disclosure of Invention
In order to solve the technical problems, the invention provides a needle washing method based on a multi-needle washing structure of a sample adding needle, which can effectively improve the cleaning cleanliness of the sample adding needle, avoid cross infection caused by the sample adding needle, and improve the precision and reliability of a detection result.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a multiple needle washing structure of a sample adding needle comprises:
the lower end of the needle washing cup is provided with a waste liquid outlet;
application of sample needle, vertical setting is in the top of washing the needle cup, and its syringe needle end can stretch into in washing the needle cup, and its key lies in:
the washing machine also comprises at least two washing liquid bottles, and at least one pneumatic pump is arranged corresponding to each washing liquid bottle;
the side wall of the needle washing cup is provided with an external flushing liquid inlet communicated with the interior of the needle washing cup;
each washing liquid bottle is communicated with the needle tail end of the sample adding needle through an inner flushing pipeline and is communicated with an outer flushing liquid inlet through an outer flushing pipeline.
By adopting the scheme, different washing liquid bottles are communicated with the inside of the sample adding needle through an inner flushing pipeline and an outer flushing pipeline and communicated with the inside of the sample adding needle cup, so that different washing liquids can be adopted to repeatedly flush the inner wall of the sample adding needle and flush the outer wall of the head end of the sample adding needle, the cleanliness of the sample adding needle is greatly improved, cross infection caused by the sample adding needle is avoided, and the precision and the reliability of detection results are ensured.
Preferably, the method comprises the following steps: the washing machine comprises a washing machine body, a washing machine, a control system and a control system, wherein the washing machine body is provided with a washing machine body, the washing machine body is provided with a washing machine;
the washing liquid bottle B is provided with a third output pipe and a fourth output pipe, the third output pipe is provided with a third switch valve, and the fourth output pipe is provided with a fourth switch valve;
the first output pipe and the third output pipe are communicated with the inner flushing pipeline, and the second output pipe and the fourth output pipe are communicated with the outer flushing pipeline.
Structure more than adopting, liquid in lotion bottle A and the lotion bottle B can be in getting into the application of sample needle alone through interior towards the pipeline, also can get into simultaneously in the interior towards the pipeline mix the back get into the application of sample needle in, can get into outer towards the liquid entry alone through outer towards the pipeline, also can get into simultaneously and mix the back and go into outer towards the liquid entry after going into in the outer towards the pipeline, can realize like this that the lotion washes the inner wall or the syringe needle end outer wall of application of sample needle, also can realize that the lotion mixes the back, utilize mixed liquid to wash the inner wall or the syringe needle end outer wall of application of sample needle, to some lotion that need the scene to be prepared, then this structure of accessible realizes, improve the cleaning efficiency greatly simultaneously guaranteeing cleaning quality.
Preferably, the method comprises the following steps: this multiple needle structure of washing of application of sample needle still includes vertical setting in the washing arm of washing needle cup one side, be equipped with the clamping part that is used for centre gripping application of sample needle on the washing arm to and the actuating mechanism of the vertical lift of drive clamping part. By adopting the structure, the sample adding needle can be conveniently stretched into the needle washing cup to be washed, and can be withdrawn from the needle washing cup after the washing is finished, and the subsequent sampling and sample adding can be carried out.
Preferably, the method comprises the following steps: the needle washing cup is characterized in that a round table protruding inwards along the radial direction of the needle washing cup is arranged in the position corresponding to the outer liquid flushing inlet, a through hole penetrating through the upper side and the lower side of the round table is vertically formed in the round table, the through hole is matched with the sample adding needle in size, and the outer liquid flushing inlet is communicated with the through hole. Structure more than adopting, when wasing, the application of sample needle can stretch into in the through-hole, can directly wash application of sample needle head end.
The key point of the method for washing the sample adding needle by multiple times is as follows: the multiple needle washing structure of the sample adding needle is adopted to wash the sample adding needle, and the washing liquid in each washing liquid bottle is different and is carried out according to the following steps:
s1: the needle head end of the lower part of the sample adding needle extends into the needle washing cup and corresponds to the position of the outer flushing liquid inlet;
s2: washing liquid is used for washing the inner wall of the sample adding needle through the inner flushing pipeline and enters the outer wall of the needle head end of the sample adding needle through the outer flushing pipeline to be washed;
s3: and the cleaning is completed, and the sample adding needle is withdrawn from the needle washing cup.
By adopting the needle washing method, the inner wall channel and the outer wall of the needle head end are washed in multiple ways, so that the washing force of the sample adding needle can be improved, the introduction of cross infection of the sample adding needle is effectively avoided, and the subsequent detection precision is improved.
Preferably, the method comprises the following steps: the washing liquid bottle A is internally provided with washing liquid a, the washing liquid bottle B is internally provided with washing liquid B, and the step S2 is carried out according to the following steps:
p 1: opening a first switch valve to enable the washing liquid a to enter the sample adding needle to wash the inner wall of the sample adding needle for the first time, and then closing the first switch valve;
p 2: opening a second switch valve to enable the washing liquid a to enter the needle washing cup through the external washing liquid inlet, washing the outer wall of the needle head end of the sample adding needle for the first time, and then closing the second switch valve;
p 3: opening the third switch valve to enable the washing liquid b to enter the sample adding needle to wash the inner wall of the sample adding needle for the second time, and then closing the third switch valve;
p 4: and opening the fourth switch valve to enable the washing liquid b to enter the needle washing cup through the outer washing liquid inlet, washing the outer wall of the needle head end of the sample adding needle for the second time, and then closing the fourth switch valve.
The steps are adopted for cleaning, so that the inner wall of the sample adding needle and the outer wall of the needle head end are ensured to be only the same washing liquid b, and the pollution caused by various washing liquids on the sample adding needle is avoided.
Preferably, the method comprises the following steps: the steps p1 and p2 may be performed simultaneously, and the steps p3 and p4 may be performed simultaneously. By adopting the needle washing method in the steps, the needle washing efficiency can be improved, and the waiting of washing liquid and the switching time can be avoided.
Preferably, the method comprises the following steps: the washing liquid bottle A is internally provided with washing liquid a, the washing liquid bottle B is internally provided with washing liquid B, and the step S2 is carried out according to the following steps:
q 1: opening a first switch valve to enable the washing liquid a to enter the sample adding needle to wash the inner wall of the sample adding needle for the first time, and then closing the first switch valve;
q 2: opening the third switch valve to enable the washing liquid b to enter the sample adding needle to wash the inner wall of the sample adding needle for the second time, and then closing the third switch valve;
q 3: opening a second switch valve to enable the washing liquid a to enter the needle washing cup through the external washing liquid inlet, washing the outer wall of the needle head end of the sample adding needle for the first time, and then closing the second switch valve;
q 4: and opening the fourth switch valve to enable the washing liquid b to enter the needle washing cup through the outer washing liquid inlet, washing the outer wall of the needle head end of the sample adding needle for the second time, and then closing the fourth switch valve.
The steps are adopted for cleaning: make lotion a and lotion b get into the application of sample needle in succession, wash its inner wall, avoid lotion a to wash its inner wall washing interval time after long, lotion b gets into again and washs, prevents that lotion a from forming the secondary dirt at application of sample needle inner wall, has avoided lotion a to form the secondary dirt at application of sample needle syringe needle tip outer wall with the reason liquid, has improved the cleaning performance of application of sample needle, reduces cleaning process and introduces secondary pollution.
Preferably, the method comprises the following steps: the washing liquid bottle A is internally provided with washing liquid a, the washing liquid bottle B is internally provided with washing liquid B, and the step S2 is carried out according to the following steps:
t 1: simultaneously opening the first switch valve and the third switch valve to enable the washing liquid a and the washing liquid b to simultaneously enter the inner flushing pipeline for mixing, enabling the mixed liquid to enter the sample adding needle to clean the inner wall of the sample adding needle, and then closing the first switch valve and the third switch valve;
t 2: and simultaneously opening the second switch valve and the fourth switch valve, simultaneously feeding the washing liquid a and the washing liquid b into the external flushing pipeline for mixing, feeding the mixed liquid into the needle washing cup through the external flushing liquid inlet, cleaning the outer wall of the needle head end of the sample adding needle, and closing the second switch valve and the fourth switch valve.
After the washing liquid a and the washing liquid b are mixed by adopting the steps, the inner wall of the sample adding needle and the outer wall of the needle head end are respectively washed, the demands of more types of washing liquids are met, the on-site mixing can be used for replacing the early-stage preparation, the time and the cost are saved, and more reagent washing purposes can be met by mixing the washing liquids.
Compared with the prior art, the invention has the beneficial effects that:
by adopting the needle washing method based on the multiple needle washing structure of the sample adding needle, which is provided by the invention, through arranging a plurality of liquid washing bottles, then the liquid washing bottles are communicated with the inside of the sample adding needle through the same inner flushing pipeline and are communicated with the inside of the needle washing cup through the same outer flushing pipeline, so that the inner wall of the sample adding needle and the outer wall of the needle head end are separately washed, mixed and washed, and washed for many times, the washing force of the sample adding needle is greatly improved, the sample adding needle is prevented from bringing cross infection, and the precision and the reliability of a subsequent detection result are ensured.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic diagram of the cleaning arm of the embodiment shown in FIG. 1.
Detailed Description
The present invention will be further described with reference to the following examples and the accompanying drawings.
Referring to fig. 1 and 2, the multiple needle washing structure for a sample injection needle mainly includes a needle washing cup 1, a sample injection needle 2, a liquid washing bottle a3, a liquid washing bottle B4, an inner flushing line 5 and an outer flushing line 6.
As shown in the figure, the needle washing cup 1 is vertically arranged on the workbench, the needle washing cup is hollow inside the workbench, a waste liquid outlet 10 is arranged at the lower end of the needle washing cup, and an external flushing liquid inlet 11 communicated with the inside of the needle washing cup 1 is arranged on the side wall of the needle washing cup;
wash the vertical cleaning arm 7 that is equipped with in one side of needle cup 1, cleaning arm 7 includes outside extension's clamping part 70 and the drive mechanism 71 that drives the vertical lift of clamping part 70, drive mechanism 71 can comprise synchronizing wheel and motor, simple structure, easily make, application of sample needle 2 passes through the centre gripping of clamping part 70, vertical setting is directly over washing needle cup 1, when drive mechanism 71 drive clamping part 70 downstream, the syringe needle end of application of sample needle 2 can stretch into and wash in the needle cup 1.
One end of the inner flushing pipeline 5 is communicated with the upper end, namely the tail end, of the sample adding needle 2, and one end of the outer flushing pipeline 6 is communicated with the outer flushing liquid inlet 11.
The washing liquid bottle A3 is provided with a first output tube 30 and a second output tube 30 ', the washing liquid bottle B4 is provided with a third output tube 40 and a fourth output tube 40', the first output tube 30 and the third output tube 40 are simultaneously communicated with one end of the inner washing pipeline 5 far away from the sample adding needle 2, and the second output tube 30 'and the fourth output tube 40' are simultaneously communicated with one end of the outer washing pipeline 6 far away from the outer washing liquid inlet 11.
In the invention, in order to better control the washing liquid inlet and outlet states of the washing liquid bottle A3 and the washing liquid bottle B4, the first output tube 30 is provided with a first switch valve 31, the second output tube 30 'is provided with a second switch valve 32, the third output tube 40 is provided with a third switch valve 41, the fourth output tube 40' is provided with a fourth switch valve 42, and a pneumatic pump 8 is arranged corresponding to the washing liquid bottle A3 and the washing liquid bottle B4, the flushing speed of the washing liquid can be controlled by the pneumatic pressure of the pneumatic pump 8, so that the flushing force can be changed.
In the invention, in order to avoid that when washing liquid is flushed into the needle washing cup 1 through the outer washing liquid inlet 11 to wash and flush the outer wall of the needle head end of the sample adding needle 2, the impact force is too large, so that the lower swing amplitude of the sample adding needle 2 is too large, the lower swing amplitude of the sample adding needle 2 collides with the needle washing cup 1 greatly, and the damage is caused, so that the structure of the needle washing cup 1 is changed, a circular truncated cone 12 is arranged in the position, corresponding to the outer washing liquid inlet 11, of the interior of the needle washing cup 1, the circular truncated cone 12 protrudes inwards from the inner wall of the needle washing cup 1 along the radial direction of the circular truncated cone 12, a through hole 120 is vertically arranged on the circular truncated cone 12, the through hole 120 penetrates through the upper side surface and the lower side surface of the circular truncated cone 12, the outer washing liquid inlet 11 extends inwards along the axial direction of the circular truncated cone 12 and is communicated with the through hole 120, the size of the.
In the invention, in order to realize pipeline sharing and save layout space and cost, the inner flushing pipeline 5 is also provided with the filling pump 50, when sampling the reagent, the reagent can be quickly sucked and filled by the filling pump, and the method is convenient and quick.
With reference to fig. 1 and 2, the needle washing method based on the multiple needle washing structure of the loading needle is mainly performed according to the following steps, firstly, the loading needle 2 is controlled to descend by the washing arm 7, the needle end at the lower part of the loading needle 2 extends into the needle washing cup 1, and the end side of the needle end faces the external flushing liquid inlet 11;
then, washing liquid enters from the tail end of the sample adding needle 2 through an inner washing pipeline 5 to clean the inner wall of the sample adding needle, the washing liquid is washed into the outer wall of the head end of the sample adding needle from an outer washing liquid inlet 11 through an outer washing pipeline 6 to be washed, and waste liquid generated during needle washing is discharged from a waste liquid outlet 10 below;
and finally, after the cleaning is finished, the sample adding needle 2 is withdrawn from the needle washing cup 1 through the cleaning arm 7, and the next procedure is carried out.
In the invention, a washing liquid bottle A3 is filled with a washing liquid a, a washing liquid bottle B4 is filled with a washing liquid B, the washing liquid a and the washing liquid B are different, and when cleaning is carried out, on the premise of following a main needle washing step, the sequence of entering the washing liquid can be controlled by a switch valve, so that more needle washing effects are met, and the main method comprises the following embodiments:
example one, under the action of the pneumatic pump 8, p 1: opening the first switch valve 31, enabling the washing liquid a to enter the sample adding needle 2 through the inner washing pipeline 5 to wash the inner wall of the sample adding needle for the first time, and then closing the first switch valve 31; p 2: opening a second switch valve 32 to enable the washing liquid a to enter the needle washing cup 1 through the external washing pipeline 6 through the external washing liquid inlet 11, realizing the first washing of the outer wall of the needle head end of the sample adding needle 2, and then closing the second switch valve 32; p 3: opening the third switch valve 41, enabling the washing liquid b to enter the sample adding needle 2 through the inner washing pipeline 5 to wash the inner wall of the sample adding needle for the second time, and then closing the third switch valve 41; p4 opens the fourth switch valve 42, the washing liquid b enters the needle washing cup 1 through the outer washing liquid inlet 11 via the outer washing pipeline 6, the second washing is realized for the outer wall of the needle head end of the sample adding needle 2, and then the fourth switch valve 42 is closed.
Example two, under the action of the pneumatic pump 8, q 1: opening the first switch valve 31, enabling the washing liquid a to enter the sample adding needle 2 to wash the inner wall of the sample adding needle for the first time, and then closing the first switch valve 31; q 2: opening the third switch valve 41 to enable the washing liquid b to enter the sample adding needle 2 to wash the inner wall of the sample adding needle for the second time, and then closing the third switch valve 41; q 3: opening a second switch valve 32, enabling the washing liquid a to enter the needle washing cup 1 through the outer washing liquid inlet 11, washing the outer wall of the needle head end of the sample adding needle 2 for the first time, and then closing the second switch valve 32; q 4: and opening the fourth switch valve 42 to enable the washing liquid b to enter the needle washing cup 1 through the outer washing liquid inlet 11, realizing the second washing on the outer wall of the needle head end of the sample adding needle 2, and then closing the fourth switch valve 42.
Example three, under the action of the pneumatic pump 8, t 1: simultaneously opening the first switch valve 31 and the third switch valve 41 to enable the washing liquid a and the washing liquid b to simultaneously enter the inner flushing pipeline 5 for mixing, enabling the mixed liquid to enter the sample adding needle 2 to clean the inner wall of the sample adding needle, and then closing the first switch valve 31 and the third switch valve 41;
t 2: and simultaneously opening the second switch valve 32 and the fourth switch valve 42, simultaneously introducing the washing liquor a and the washing liquor b into the external flushing pipeline 6 for mixing, introducing the mixed liquor into the needle washing cup 1 through the external flushing liquor inlet 11, cleaning the outer wall of the needle head end of the sample adding needle 2, and closing the second switch valve 32 and the fourth switch valve 42.
In the first embodiment, the washing liquid a and the washing liquid b enter the sample adding needle 2 at intervals to clean the inner wall of the sample adding needle, and the outer wall of the needle head end is cleaned at intervals, so that the washing liquid a has enough retention time, and finally, the washing liquid b is attached to the sample adding needle 2 completely, and the overall purity of the sample adding needle 2 is ensured.
The main difference between the second embodiment and the first embodiment is that the two steps of p2 and p3 in the first embodiment are exchanged, so that the washing can avoid the situation that the washing solution a stays on the sampling needle 2 for too long time, and secondary dirt is prevented from forming on the sampling needle 2, so that the washing solution b cannot be washed, and the selection of the first embodiment and the second embodiment is determined according to the chemical property of the washing solution and the chemical property of the reagent carried by the sampling needle 2;
when cleaning, because the processes of washing the inner wall of the sample adding needle 2 and washing the outer wall of the needle head end are not interfered with each other, on the premise of ensuring the cleaning efficiency, the steps p1 and p2 in the first embodiment can be simultaneously carried out, the steps p3 and p4 can be simultaneously carried out, and in the second embodiment with the same structure, the steps q1 and q3 and the steps q2 and q4 can be simultaneously carried out, so that the waiting time of the cleaning solution is greatly reduced, and the cleaning efficiency is improved.
In the third embodiment, the case that the sample addition needle needs to be cleaned by the mixed washing solution is considered, for example, the sample addition needle 2 needs to be cleaned by a certain mixed washing solution, and the mixed washing solution needs to be cleaned by the washing solution a and the washing solution b, if the mixed washing solution is mixed in advance, the performance is weakened after a long time, and the temporary mixing preparation effect is not good, so that the third embodiment can realize quick mixing and immediate cleaning of the mixed washing solution, and improve the cleaning effect of the mixed washing solution.
In the embodiment, only two washing liquid bottles are taken as reference, in the actual production process, a plurality of washing liquid bottles can be arranged according to the washing requirements, different washing liquids are filled in the washing liquid bottles, each washing liquid bottle is provided with two output pipes, one of the output pipes can be communicated with the inner flushing pipeline 5, and the other output pipe is communicated with the outer flushing pipeline 6;
the following purposes can be achieved: any one of the washing liquids can enter the sample adding needle 2 through the inner flushing pipeline 5 to wash the inner wall of the sample adding needle, or any at least two of the washing liquids can enter the inner flushing pipeline 5 simultaneously to be mixed and then enter the sample adding needle 2 to wash the inner wall of the sample adding needle, any one of the washing liquids can wash the outer wall of the needle end of the sample adding needle 2 in the sample adding needle cup 1 after passing through the outer flushing pipeline 6, or any at least two of the washing liquids can enter the outer flushing pipeline 6 simultaneously to be mixed and then wash the outer wall of the needle end of the sample adding needle 2 in the sample adding needle cup 1, and the washing steps can be actually adjusted according to the chemical properties of the washing liquids or the requirements of the finally attached washing liquids.
Finally, it should be noted that the above-mentioned description is only a preferred embodiment of the present invention, and those skilled in the art can make various similar representations without departing from the spirit and scope of the present invention.
Claims (3)
1. The utility model provides a wash needle method based on multiple needle structure of washing of application of sample needle adopts multiple needle structure of washing of application of sample needle washs application of sample needle (2), its characterized in that, wherein the multiple needle structure of washing of application of sample needle includes:
the lower end of the needle washing cup (1) is provided with a waste liquid outlet (10);
the sample adding needle (2) is vertically arranged above the needle washing cup (1), and the needle head end of the sample adding needle can extend into the needle washing cup (1);
the washing machine is characterized by also comprising a washing liquid bottle A (3) and a washing liquid bottle B (4), wherein at least one pneumatic pump (8) is arranged corresponding to the washing liquid bottle A (3) and the washing liquid bottle B (4), the washing liquid bottle A (3) is internally provided with washing liquid a, and the washing liquid bottle B (4) is internally provided with washing liquid B;
the side wall of the needle washing cup (1) is provided with an external flushing liquid inlet (11) communicated with the interior of the needle washing cup;
the washing liquid bottle A (3) and the washing liquid bottle B (4) are both communicated with the needle tail end of the sample adding needle (2) through an inner flushing pipeline (5) and communicated with an outer flushing liquid inlet (11) through an outer flushing pipeline (6);
the filling pump (50) is arranged on the inner flushing pipeline (5), the cleaning arm (7) is arranged on one side of the needle washing cup (1), the cleaning arm (7) is provided with a clamping part (70) for clamping the sampling needle (2), and a driving mechanism (71) for driving the clamping part (70) to vertically lift;
the washing liquid bottle A (3) is provided with a first output pipe (30) and a second output pipe (30 '), the first output pipe (30) is provided with a first switch valve (31), and the second output pipe (30') is provided with a second switch valve (32);
the washing bottle B (4) is provided with a third output pipe (40) and a fourth output pipe (40 '), the third output pipe (40) is provided with a third switch valve (41), and the fourth output pipe (40') is provided with a fourth switch valve (42);
the first output pipe (30) and the third output pipe (40) are communicated with the inner flushing pipe (5), and the second output pipe (30 ') and the fourth output pipe (40') are communicated with the outer flushing pipe (6);
the needle washing method comprises the following steps of,
s1: the needle head end of the lower part of the sample adding needle (2) extends into the needle washing cup (1) and corresponds to the position of the external flushing liquid inlet (11);
s2: washing liquor enters the inner wall of the sample adding needle (2) through the inner flushing pipeline (5) and washes the outer wall of the needle head end of the sample adding needle (2) through the outer flushing pipeline (6);
s3: after the cleaning is finished, the sample adding needle (2) is withdrawn from the needle washing cup (1);
wherein, the step S2 can be performed as follows,
p 1: opening a first switch valve (31) to enable the washing liquid a to enter the sample adding needle (2) to wash the inner wall of the sample adding needle for the first time, and then closing the first switch valve (31);
p 2: opening a second switch valve (32) to enable the washing liquid a to enter the needle washing cup (1) through the outer washing liquid inlet (11), realizing first washing on the outer wall of the needle head end of the sample adding needle (2), and then closing the second switch valve (32);
p 3: opening the third switch valve (41) to enable the washing liquid b to enter the sample adding needle (2) to wash the inner wall of the sample adding needle for the second time, and then closing the third switch valve (41);
p 4: opening a fourth switch valve (42), enabling washing liquor b to enter the needle washing cup (1) through an outer washing liquor inlet (11), washing the outer wall of the needle head end of the sample adding needle (2) for the second time, and then closing the fourth switch valve (42);
step S2 may also be performed as follows,
q 1: opening a first switch valve (31) to enable the washing liquid a to enter the sample adding needle (2) to wash the inner wall of the sample adding needle for the first time, and then closing the first switch valve (31);
q 2: opening the third switch valve (41) to enable the washing liquid b to enter the sample adding needle (2) to wash the inner wall of the sample adding needle for the second time, and then closing the third switch valve (41);
q 3: opening a second switch valve (32) to enable the washing liquid a to enter the needle washing cup (1) through the outer washing liquid inlet (11), realizing first washing on the outer wall of the needle head end of the sample adding needle (2), and then closing the second switch valve (32);
q 4: opening a fourth switch valve (42), enabling washing liquor b to enter the needle washing cup (1) through an outer washing liquor inlet (11), washing the outer wall of the needle head end of the sample adding needle (2) for the second time, and then closing the fourth switch valve (42);
step S2 may also be performed as follows,
t 1: simultaneously opening a first switch valve (31) and a third switch valve (41) to enable the washing liquid a and the washing liquid b to simultaneously enter an inner flushing pipeline (5) for mixing, enabling the mixed liquid to enter a sample adding needle (2) to clean the inner wall of the sample adding needle, and then closing the first switch valve (31) and the third switch valve (41);
t 2: and simultaneously opening a second switch valve (32) and a fourth switch valve (42), simultaneously introducing the washing liquor a and the washing liquor b into an external flushing pipeline (6) for mixing, introducing the mixed liquor into the needle washing cup (1) through an external flushing liquor inlet (11), cleaning the outer wall of the needle head end of the sample adding needle (2), and closing the second switch valve (32) and the fourth switch valve (42).
2. The needle washing method based on the sample adding needle multiple needle washing structure as claimed in claim 1, wherein: the steps p1 and p2 may be performed simultaneously, and the steps p3 and p4 may be performed simultaneously.
3. The needle washing method based on the sample adding needle multiple needle washing structure as claimed in claim 1, wherein: the needle washing cup is characterized in that a circular truncated cone (12) protruding inwards in the radial direction is arranged in the position, corresponding to the outer flushing inlet (11), in the needle washing cup (1), a through hole (120) penetrating through the upper side and the lower side of the circular truncated cone (12) is vertically arranged on the circular truncated cone (12), the through hole (120) is matched with the sample adding needle (2) in size, and the outer flushing inlet (11) is communicated with the through hole (120).
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| CN201710752586.0A CN107695046B (en) | 2017-08-28 | 2017-08-28 | Needle washing method based on multiple needle washing structures of sample adding needle |
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| CN111570422A (en) * | 2019-02-18 | 2020-08-25 | 深圳迎凯生物科技有限公司 | Sampling needle cleaning device and method |
| CN110252741B (en) * | 2019-06-18 | 2020-09-04 | 珠海丽珠试剂股份有限公司 | Cleaning control method, device and system for sampling needle |
| CN110261629A (en) * | 2019-07-26 | 2019-09-20 | 重庆德方信息技术有限公司 | Dropping liquid cleaning mechanism for health test apparatus |
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| GB2075672B (en) * | 1980-04-17 | 1983-03-09 | Chemlab Mfg Ltd | Washpot for use with sampling probe |
| JP4992401B2 (en) * | 2006-12-06 | 2012-08-08 | 株式会社島津製作所 | Autosampler cleaning mechanism |
| JP5449967B2 (en) * | 2009-10-05 | 2014-03-19 | 日立アロカメディカル株式会社 | Nozzle cleaning unit |
| CN102632057B (en) * | 2012-03-21 | 2015-02-04 | 北京博晖创新光电技术股份有限公司 | Cleaning device for capillary tube of automatic sampler and cleaning method |
| CN103389265A (en) * | 2013-07-30 | 2013-11-13 | 济南华天恒达科技有限公司 | Sampling and washing system |
| CN104690047B (en) * | 2013-12-10 | 2016-08-24 | 中国科学院大连化学物理研究所 | A kind of liquid chromatograph sample intake passage cleans device |
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