CN107576544B - Microporous filter membrane elution device and elution method - Google Patents

Abstract

The invention discloses a microporous filter membrane elution device and an elution method, and the microporous filter membrane elution device comprises a shell, an ultrasonic generator, a pump, at least one tube seat and at least one elution tube, wherein the ultrasonic generator is arranged at the bottom of the shell and used for providing ultrasonic oscillation; the tube seat is arranged on the ultrasonic generator and provides a space for containing the elution tube; the elution pipe comprises an upper section and a lower section, the upper section and the lower section are detachably connected, the bottom of the upper section is provided with a filter element, an outlet of the filter element is provided with a latex port, and the latex port is opened under the action of a set suction force; the bottom of the lower section, which is located on the filter element, is provided with at least one opening, and when the elution tube is placed in the tube seat, the opening is connected with a pump arranged in the shell and used for providing negative pressure for the interior of the lower section. The elution device can realize elution of substances to be detected on the microporous filter membrane at a high speed so as to solve the problem that the microporous filter membrane is broken due to overlong soaking time of the microporous filter membrane and further avoid complex filtering procedures.

Description

A kind of microporous membrane elution device and elution method

technical field

The invention belongs to the technical field of environmental protection and occupational hygiene detection, and particularly relates to a microporous filter membrane elution device and an elution method.

Background technique

At present, the method for the determination of soluble aerosol potassium (sodium) and compounds in the air is to collect the air sample at the sampling point with a sampling clip with a microporous membrane installed at a flow rate of 5 L/min for 15 minutes. Fold the dust side inward for 2 times, put it into a colorimetric tube with a plug, and then use an ion chromatograph or a flame atomic absorption spectrometer to measure it. The pore size of the microporous filter membrane is 0.8 μm. The commonly used elution method is to put the sampled filter membrane into a colorimetric tube with a stopper, add 10.0 ml of water, and elute for 10 minutes. Eluent for measurement. This detection method has the following drawbacks: 1. The microporous membrane soaking time is too long, and it is easy to be broken. The eluent needs to be filtered before the instrument is used for measurement, which is cumbersome to operate, and the eluent is easily contaminated during the transfer filtration process. affect the detection accuracy. 2. The stoppered colorimetric tube is made of glass, which is easy to absorb metal ions including potassium, sodium, lead, cadmium and other metal ions in water. The storage time is short, and the test results will be low if left overnight.

SUMMARY OF THE INVENTION

In view of the technical problems existing in the above-mentioned prior art, the purpose of the present invention is to provide a microporous membrane elution device and an elution method. The elution device can realize the elution of the substance to be tested on the microporous filter membrane at a relatively fast speed, so as to solve the breakage of the microporous filter membrane caused by the long soaking time of the microporous filter membrane, thereby avoiding complicated filtration procedures. The filtrate can be detected by quickly filtering the eluate, and the filtrate does not need to be transferred multiple times, thereby avoiding secondary pollution of the filtrate.

In order to solve the above technical problems, the technical scheme of the present invention is:

A microporous membrane elution device, comprising a shell, an ultrasonic generator, a pump, at least one tube seat and at least one elution pipe, wherein the ultrasonic generator is installed at the bottom of the shell for providing ultrasonic vibration ;

The tube seat is installed on the ultrasonic generator to provide a space for holding the elution tube;

The elution pipe comprises an upper section and a lower section, the upper section and the lower section are detachably connected, a filter element is arranged at the bottom of the upper section, a latex port is arranged at the outlet of the filter element, and the latex port is opened under the action of greater than the set suction;

At least one opening is provided at the bottom of the filter element in the lower section. When the elution pipe is placed in the tube base, the opening is connected to the pump installed in the housing to provide negative pressure inside the lower section.

During elution, place the microporous filter membrane in the upper section of the elution tube, add the eluent to the upper section, place the elution tube in the tube seat, turn on the ultrasonic generator, and perform ultrasonic vibration on the elution tube. , to accelerate the elution of the analyte on the microporous membrane. In a short elution time, the microporous filter membrane will not be broken and will not pollute the eluent, so there is no need to carry out a more complicated filtration procedure. After the elution is completed, the pump is turned on, and the pump evacuates the lower section through the opening on the lower section to provide negative pressure. The latex port at the lower end of the filter element is opened under the action of suction, and the eluent flows into the lower section after filtration to achieve rapid filtration. Rapid filtration can solve the problem of slow filtration in traditional filtration methods and easy to be polluted by external pollutants. The structure of the lower section is also tubular, similar to a test tube, and its volume is small. When testing the filtrate, you can directly disassemble the upper and lower sections, and directly hold the lower section for testing, which solves the need for traditional methods (such as suction filtration). The filtrate can only be detected when it is transferred, and it is easy to cause secondary pollution to the filtrate during the transfer process.

Preferably, the material of the elution tube is polyethylene. On the one hand, polyethylene does not adsorb or exude metal ions, which can ensure the accuracy of the test results; on the other hand, it can prevent the elution tube from being broken during ultrasonic vibration.

Preferably, the filter element comprises a funnel and a filter paper attached to the funnel, and the latex port is arranged at the outlet of the funnel.

During elution, directly place the eluent and microporous filter paper on top of the filter paper, and after the elution by ultrasonic vibration is completed, rapid filtration can be performed.

Further preferably, the latex mouth is a cigarette holder-shaped latex mouth.

Preferably, the upper section and the lower section of the elution pipe are connected by threads. The threaded connection can ensure the firmness of the connection between the upper section and the lower section, and can ensure the sealing performance of the lower section and ensure the smooth progress of negative pressure filtration.

Preferably, the top of the upper section of the elution tube is covered with a sealing cap. The sealing cap is convenient to seal the upper section to avoid splashing of the eluent during the elution process.

Preferably, the top of the casing is open, and the top of the casing is provided with a box cover. When idle, the box cover can isolate dust, and when in use, the box cover can reduce the noise of the machine.

Preferably, the number of the sockets is 10-30, preferably 16-24. The sockets are evenly distributed in the chamber of the shell, and can be arranged in the form of concentric circles or in rows.

The elution method of the above-mentioned microporous membrane elution device comprises the following steps:

Put the sampled microporous membrane into the upper section of the elution tube, add the eluent to the upper section of the elution tube, and tighten the sealing cap; then place the elution tube in the tube seat inside the shell, and start the ultrasonic wave. shaker for rapid elution;

After the elution is completed, turn on the pump, evacuate the lower section of the elution tube, and filter the eluent under negative pressure;

After the filtration, the upper and lower sections of the elution tube were disassembled, and the eluate in the lower section was detected.

Preferably, the rapid elution time is 3-5 min.

The eluent is water.

The beneficial effects of the present invention are:

During elution, place the microporous filter membrane in the upper section of the elution tube, add the eluent to the upper section, place the elution tube in the tube seat, turn on the ultrasonic generator, and perform ultrasonic vibration on the elution tube. , to accelerate the elution of the analyte on the microporous membrane. In a short elution time, the microporous filter membrane will not be broken and will not pollute the eluent, so there is no need to carry out a more complicated filtration procedure. After the elution is completed, the pump is turned on, and the pump evacuates the lower section through the opening on the lower section to provide negative pressure. The latex port at the lower end of the filter element is opened under the action of suction, and the eluent flows into the lower section after filtration to achieve rapid filtration. Rapid filtration can solve the problem of slow filtration in traditional filtration methods and easy to be polluted by external pollutants. The structure of the lower section is also tubular, similar to a test tube, and its volume is small. When testing the filtrate, you can directly disassemble the upper and lower sections, and directly hold the lower section for testing, which solves the need for traditional methods (such as suction filtration). The filtrate can only be detected when it is transferred, and it is easy to cause secondary pollution to the filtrate during the transfer process.

Description of drawings

The accompanying drawings that form a part of the present application are used to provide further understanding of the present application, and the schematic embodiments and descriptions of the present application are used to explain the present application and do not constitute improper limitations on the present application.

Fig. 1 is the overall structure schematic diagram of the elution device of the present invention;

Fig. 2 is the top view structure schematic diagram of the elution device of the present invention;

Fig. 3 is the structural representation of the elution tube of the present invention;

FIG. 4 is a schematic structural diagram of the filtering structure of the present invention.

Among them, 1. Shell, 2. Tank cover, 3. Tube base, 4. Elution tube, 5. Upper section, 6. Lower section, 7. Thread, 8. Filter element, 9. Sealing cover, 10. Funnel, 11 , filter paper, 12, opening one, 13, latex mouth, 14, opening two.

Detailed ways

It should be noted that the following detailed description is exemplary and intended to provide further explanation of the application. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

It should be noted that the terminology used herein is for the purpose of describing specific embodiments only, and is not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural as well, furthermore, it is to be understood that when the terms "comprising" and/or "including" are used in this specification, it indicates that There are features, steps, operations, devices, components and/or combinations thereof.

As shown in Figure 1, the invention is composed of a box body 1 and a box cover 2. The box cover 2 is closed on the box body 1 and can be used to prevent dust when not in use, and can be used to prevent noise during normal use. As shown in Figure 2, there are multiple rows of tube seats 3 inside the box body 1, and the elution tubes 4 can be fixed. The number of rows of tube seats 3 is 4, and the number of tube seats 3 in each row is 6. Arrange them in an arrangement, such as in concentric circles.

An ultrasonic generator is installed at the bottom of the tube holder 3 to generate ultrasonic vibration, which is convenient for rapid elution. A vacuum pump is also installed in the housing 1, a hole is opened on the inner wall of the tube seat 3, and a pipeline is arranged. One end of the pipeline is connected to the vacuum pump, and the other end extends out of the inner wall of the tube seat 3. When the elution tube 4 is placed on the tube seat 3, the pipeline can be connected to the corresponding part on the elution tube 4, and the elution tube 4 can be filtered under negative pressure.

As shown in FIG. 3 , the elution pipe 4 is made of hard polyethylene and consists of an upper section 5 and a lower section 6 , and the upper end of the upper section 5 is covered with a sealing cover 9 . The upper section 5 and the lower section 6 are connected by the thread 7. During the elution and filtration, the upper section 5 and the lower section 6 are fixedly connected by the thread, and then the sealing cover 9 is screwed on the upper end of the upper section 5, and the liquid does not overflow; Unscrew and separate from the lower section 6. A filter element 8 is provided at the bottom of the upper section of the elution tube. As shown in FIG. 4 , the filter element 8 includes a funnel 10 and a filter paper 11 attached to the inner wall of the funnel 10 , and the latex port is provided at the outlet of the funnel 10 . The wall at the bottom of the filter element 8 of the lower section 6 is provided with an opening 12 and an opening 2 14. The opening 12 and the opening 2 14 are symmetrically arranged on both sides of the wall of the lower section 6, and the elution tube 4 is placed in the tube seat 3. At the time, the opening one 12 and the opening two 14 are respectively matched with the pipes extending from the inner wall of the pipe seat 3. Because the diameter of the pipe seat 3 is limited, the pipes are preferably made of flexible materials. Under the extrusion action of the elution pipe 4, the pipes and the For the sealing between the outer walls of the lower section 6, when the vacuum pump is started, the interior of the lower section 6 of the elution pipe 4 can be evacuated through the opening 12 and the opening 14. The latex port 13 is opened under the action of suction, which is convenient for vacuum filtration.

Put the sampled microporous filter membrane into the elution tube 4, add 10.0 ml of laboratory water, tighten the sealing cap 9, and place it on the base 3 of the shaking elution machine. Turn on the power, select the oscillation function, and adjust the amplitude and time. According to many experiments, the vibration time of the filter membrane for elution and collection of potassium and sodium elements in the laboratory is now selected as 5 minutes. After the end, select the function key to the quick filter key, and the eluent is filtered through the filter element 8 to the lower section 6 of the elution tube. After the filtration, the upper section 5 and the lower section 6 are unscrewed and separated, and the eluate in the lower section 6 is directly detected by the instrument.

The above descriptions are only preferred embodiments of the present application, and are not intended to limit the present application. For those skilled in the art, the present application may have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of this application shall be included within the protection scope of this application.

Claims (2)

1. an elution method of a microporous membrane elution device, characterized in that: the device used in the elution method comprises a casing, an ultrasonic generator, a pump, at least one tube seat and at least one elution pipe, wherein , the ultrasonic generator is installed at the bottom of the casing to provide ultrasonic vibration; The tube seat is installed on the ultrasonic generator to provide a space for holding the elution tube; The elution pipe comprises an upper section and a lower section, the upper section and the lower section are detachably connected, a filter element is arranged at the bottom of the upper section, a latex port is arranged at the outlet of the filter element, and the latex port is opened under the action of greater than the set suction; The lower section is provided with at least one opening at the bottom of the filter element, and when the elution pipe is placed in the tube seat, the opening is connected to the pump installed in the housing to provide negative pressure to the interior of the lower section; The material of the elution pipe is polyethylene; The filter element comprises a funnel and a filter paper attached to the funnel, and the latex port is arranged at the outlet of the funnel; The latex mouth is a cigarette holder-shaped latex mouth; The upper section and the lower section of the elution pipe are connected by threads; The top of the upper section of the elution tube is covered with a sealing cap; The top of the casing is open, and the top of the casing is provided with a box cover; The number of sockets is 10-30; The elution method includes the following steps: placing the sampled microporous filter membrane into the upper section of the elution tube, adding eluent to the upper section of the elution tube, and tightening the sealing cover; then placing the elution tube in the shell In the tube seat inside the body, start the ultrasonic generator to perform rapid elution; after the elution is completed, turn on the pump, vacuum the lower part of the elution tube, and filter the eluent under negative pressure; after the filtration, the elution The upper and lower sections of the tube are disassembled, and the eluate in the lower section is detected; The rapid elution time is 3-5min. 2 . The elution method according to claim 1 , wherein the number of the tube holders is 16-24. 3 .

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