CN107568242A - A kind of composition pesticide for preventing and treating Diptera subterranean pest-insect and preparation method and application - Google Patents

Abstract

The invention belongs to the technical field of pesticide preparations, in particular to a pesticide composition for preventing and controlling diptera underground pests, and further discloses its preparation method and application. The pesticide composition for controlling Diptera underground pests according to the present invention uses chlorfenapyr and thiamethoxam as main components, and utilizes the advantages of the combination of the two to have a significant synergistic effect on the 3rd instar larvae of T. effect. The active ingredients of the two agents have different mechanisms of action, and their combined use not only has a good insecticidal effect, no cross-resistance, but also can slow down the generation of insecticide resistance in pests. Compared with the use of a single chemical, it not only improves the insecticidal toxicity, but also reduces the dosage of a single chemical, saves control costs and effectively reduces pesticide residues, reduces environmental pollution, and improves food safety. It can be used as a An important means of integrated pest control.

Description

Pesticide composition for controlling diptera subterranean pests and its preparation method and application

technical field

The invention belongs to the technical field of pesticide preparations, in particular to a pesticide composition for preventing and controlling diptera underground pests, and further discloses its preparation method and application.

Background technique

Bradysia odoriphaga Yang et Zhang, whose larvae are commonly known as chive maggots, belong to Diptera, Odoriphagae family. It mainly harms leeks, scallions, onions, shallots, garlic and other Liliaceae vegetables. Occasionally, it also harms more than 30 kinds of vegetables in seven families such as Compositae, Chenopodiaceae, Cruciferae, Cucurbitaceae, Umbelliferae, etc. , can also harm edible fungi, and is one of the main pests of onion and garlic vegetables. Chive tardigrades in my country are mainly distributed in Beijing, Tianjin, Shandong, Shanxi, Liaoning, Jiangxi, Ningxia, Inner Mongolia, Zhejiang, Taiwan and other places.

The larvae have adult, egg, larvae, and pupae. The larvae gather in the bulbs and soft stems of the leek, causing them to rot and even die in pieces; the newly hatched larvae first damage the base of the leek leaf sheath and the upper end of the bulb; in spring and autumn, it mainly harms the young stems of leeks and causes rot, causing the leaves to turn yellow and die; in summer, the larvae move downward and eat into the bulbs. In severe cases, the bulbs rot and the whole pile of leeks dies. Leek maggots are generally hidden at a depth of 0-5cm below the surface, and some even invade into the rhizome, which is affected by important factors such as the protection of the soil and the rhizome of the leek or uneven application of pesticides, making it difficult for the pesticide to contact the worm body, which affects the effectiveness of chemical agents. prevention effect.

In the current agricultural production process, chemical pesticides are still the most commonly used control methods. In order to prevent leek maggots, vegetable farmers often use pesticides frequently or in excess, and even use highly toxic or highly toxic chemical pesticides. However, with the long-term and large-scale application of chemical pesticides, not only the resistance of pests continues to increase, but also the control of pests becomes more and more difficult, the control effects of various pesticides gradually decrease, and the cost of control is also increasing; Exceeding the standard will cause great hidden dangers to food safety, and lead to serious pollution of soil and water sources, severe damage to the ecological environment, and great threats and hidden dangers to the environment.

Contents of the invention

Therefore, the technical problem to be solved by the present invention is to provide a pesticide composition for controlling Diptera underground pests, so as to solve the problem of poor control effect of Diptera underground pests in the prior art.

The second technical problem to be solved by the present invention is to provide a pesticide preparation containing the pesticide composition as an active ingredient.

In order to solve the above technical problems, a pesticide composition for controlling Diptera subterranean pests according to the present invention comprises chlorfenapyr and thiamethoxam in a mass ratio of 1:0.32-26.

The mass ratio of chlorfenapyr and thiamethoxam is 1:0.32-4.

The invention also discloses the use of the pesticide composition for preparing pesticide preparations for controlling Diptera subterranean pests.

The invention also discloses a pesticide preparation for controlling Diptera subterranean pests. The pesticide preparation uses the pesticide composition as an active ingredient, and based on the total amount of the pesticide preparation, the mass of the pesticide composition is The content is 3.9-28.2wt%.

Preferably, the pesticide formulation also includes an emulsifier and a solvent.

Specifically, the emulsifier includes Nongru 500 and/or Nongru 1601; the solvent includes acetone and/or N,N-dimethylformamide.

More preferably, the pesticide formulation, based on the total amount of the pesticide formulation, includes the following components in mass content:

Chlorfenapyr 0.7-10wt%;

Thiamethoxam 3.2-18.2wt%;

Emulsifier 3-20wt%;

Solvent balance.

Optimally, based on the total amount of the pesticide formulation, it includes the following components in mass content:

Chlorfenapyr 3-10wt%;

Thiamethoxam 3.2-12wt%;

Emulsifier 3-20wt%;

Solvent balance.

The Diptera subterranean pests include tardyphagia chives.

The invention also discloses a method for preparing the pesticide preparation, which includes the steps of mixing and dissolving selected amounts of the chlorfenapyr and thiamethoxam technical materials.

Specifically, the preparation method includes the following steps: taking a selected amount of the original drug of chlorfenapyr and adding it to a part of the selected solvent, fully stirring and dissolving; then adding a selected amount of emulsifier and mixing while stirring; After the original drug is dissolved, add the original drug of thiamethoxam, stir until the thiamethoxam is dissolved, add the remaining solvent, mix and shake well, and obtain a uniform and transparent mixed preparation.

The pesticide composition for preventing and controlling Diptera pests according to the present invention uses chlorfenapyr and thiamethoxam as the main components, both of which are high-efficiency and low-toxic insecticides in the prior art, wherein,

Chlorfenapyr, commonly known as chlorfenapyr in Chinese, belongs to arylpyrrole compounds, the pure product is white or off-white oily powder, and the chemical formula is 4-bromo-2-(4-chlorophenyl)-1-ethoxy Methyl-5-trifluoromethylpyrrole-3-carbonitrile, the structural formula is Chlorfenapyr is an insecticide precursor with strong systemic properties. Its insecticidal mechanism is to block the oxidative phosphorylation of mitochondria, and it has strong stomach toxicity and certain contact killing effect on pests. It has a long duration of action, is safe for crops, and has low toxicity to humans and animals. At the same time, it has no resistance when used with other agents. It is a kind of insecticide and acaricide that has attracted much attention;

Thiamethoxam belongs to neonicotinoid insecticides, the appearance of the original drug is grayish yellow to white crystalline powder, the chemical name is 3-(2-chloro-1,3-thiazol-5-ylmethyl)-5-methyl -1,3,5-Oxadiazin-4-ylidene(nitro)amine, the molecular formula is It can inhibit the nicotinic acid acetylcholinesterase receptor in the central nervous system of insects, thereby blocking the normal conduction of the central nervous system. It has gastric toxicity, contact killing and systemic activity to pests, and has high efficiency, low toxicity, long-lasting effect, and insecticidal spectrum. Wide and other characteristics, is a widely used insecticide.

The pesticide composition of the present invention is used as a mixture of the above two agents, and by combining the advantages of the two, it has a significant synergistic effect on the 3rd instar larvae of T. tardigrades. The active ingredients of the two agents have different mechanisms of action, and their combined use not only has a good insecticidal effect, no cross-resistance, but also can slow down the generation of insecticide resistance in pests. Compared with the use of a single chemical, it not only improves the insecticidal toxicity, but also reduces the dosage of a single chemical, saves control costs and effectively reduces pesticide residues, reduces environmental pollution, and improves food safety. It can be used as a An important means of integrated pest control.

The pesticide preparation for preventing and controlling Diptera pests of the present invention, with the pesticide composition as an active ingredient, can be effectively used for the prevention and control of Diptera underground pests, especially for the prevention and control of tardigrade larvae of chives. Effect.

detailed description

In the following embodiments and experimental examples of the present invention:

The original drug of chlorfenapyr was purchased from Zhongnong United Pesticide Co., Ltd.;

The original drug of thiamethoxam was purchased from Jiangsu Luye Agrochemical Co., Ltd.;

The leek maggots to be tested were reared in the planting and maintenance room of Shandong Academy of Agricultural Sciences for sensitive populations. The breeding temperature was (25±1)°C and the relative humidity was (75±5)%. The 3rd instar larvae were selected as standard test insects.

Example 1

The pesticide composition for controlling Bradyphagia chinensis described in this example includes chlorfenapyr and thiamethoxam at a mass ratio of 1:26.

The pesticide preparation for the prevention and treatment of tardimethoxetus described in the present embodiment, in terms of the total amount of the preparation, includes the following components in mass content: chlorfenapyr 0.7wt%, thiamethoxam 18.2wt%, emulsifier (nongru 500) 5wt%, and the balance is solvent (N,N-dimethylformamide).

The pesticide preparation for the prevention and treatment of Chive tardigrade described in this embodiment is prepared according to the following method: take a selected amount of the original drug of chlorfenapyr and add it to a part of the selected solvent (1/2 amount), fully stir and dissolve; then Add a selected amount of emulsifier and mix evenly while stirring; after the original drug of chlorfenapyr is dissolved, add a selected amount of original drug of thiamethoxam, stir until thiamethoxam is dissolved, add the remaining solvent, mix and shake well, and obtain a uniform Transparent compound formulation.

Example 2

The pesticide composition for controlling Bradyphagia chinensis described in this example includes chlorfenapyr and thiamethoxam in a mass ratio of 1:12.

The pesticide preparation for the prevention and treatment of tardimethoxetus described in the present embodiment, based on the total amount of the preparation, includes the following components in mass content: chlorfenapyr 0.7wt%, thiamethoxam 8.4wt%, emulsifier (Nongru 500) 10wt%, and the balance is solvent (acetone).

The preparation method of the pesticide preparation for preventing and treating tardigrade maggots described in this example is the same as that in Example 1.

Example 3

The pesticide composition for controlling Bradyphagia chinensis described in this example includes chlorfenapyr and thiamethoxam in a mass ratio of 1:7.

The pesticide preparation for the prevention and treatment of Bradyphagia leek described in this example, based on the total amount of the preparation, includes the following components in mass content: chlorfenapyr 1wt%, thiamethoxam 7wt%, emulsifier (Nongru 1601) 15 wt%, and the balance is solvent (acetone).

The preparation method of the pesticide preparation for preventing and treating tardigrade maggots described in this example is the same as that in Example 1.

Example 4

The pesticide composition for controlling Bradyphagia chinensis described in this example includes chlorfenapyr and thiamethoxam in a mass ratio of 1:4.

The pesticide preparation for the prevention and treatment of Brachycephalus leek described in the present embodiment, based on the total amount of the preparation, includes the following components in mass content: chlorfenapyr 3wt%, thiamethoxam 12wt%, emulsifier (Nongru 500) 20 wt%, and the balance is solvent (acetone).

The preparation method of the pesticide preparation for preventing and treating tardigrade maggots described in this example is the same as that in Example 1.

Example 5

The pesticide composition for controlling Bradyphagia chinensis described in this example includes chlorfenapyr and thiamethoxam in a mass ratio of 1:3.

The pesticide preparation for the prevention and treatment of tardimethoxetus described in this example, based on the total amount of the preparation, includes the following components in mass content: chlorfenapyr 5wt%, thiamethoxam 15wt%, emulsifier (Nongru 1601) 17wt%, and the balance is solvent (N,N-dimethylformamide).

The preparation method of the pesticide preparation for preventing and treating tardigrade maggots described in this example is the same as that in Example 1.

Example 6

The pesticide composition for controlling Bradyphagia chinensis described in this example includes chlorfenapyr and thiamethoxam in a mass ratio of 1:2.

The pesticide preparation for the prevention and treatment of Bradylopia japonica described in the present embodiment, based on the total amount of the preparation, includes the following components in mass content: chlorfenapyr 9wt%, thiamethoxam 18wt%, emulsifier (Nongru 500) 7wt%, and the balance is solvent (N,N-dimethylformamide).

The preparation method of the pesticide preparation for preventing and treating tardigrade maggots described in this example is the same as that in Example 1.

Example 7

The pesticide composition for controlling Bradyphagia chinensis described in this example includes chlorfenapyr and thiamethoxam in a mass ratio of 13:16.

The pesticide preparation for the prevention and treatment of tardimethoxetus described in the present embodiment, based on the total amount of the preparation, includes the following components in mass content: chlorfenapyr 6.5wt%, thiamethoxam 8wt%, emulsifier (Nongru 1601 ) 8wt%, and the balance is solvent (N,N-dimethylformamide).

The preparation method of the pesticide preparation for preventing and treating tardigrade maggots described in this example is the same as that in Example 1.

Example 8

The pesticide composition for controlling Tardimethoxetus described in this example includes chlorfenapyr and thiamethoxam in a mass ratio of 11:8.

The pesticide preparation for the prevention and treatment of tardimethoxetus described in the present embodiment, based on the total amount of the preparation, includes the following components in mass content: chlorfenapyr 5.5wt%, thiamethoxam 4wt%, emulsifier (Nongru 1601 ) 3wt%, and the remainder is solvent (acetone).

The preparation method of the pesticide preparation for preventing and treating tardigrade maggots described in this example is the same as that in Example 1.

Example 9

The pesticide composition for controlling Bradyphagia chinensis described in this example includes chlorfenapyr and thiamethoxam in a mass ratio of 1:0.32.

The pesticide preparation for the prevention and treatment of Brachycephalus leek described in the present embodiment, in terms of the total amount of the preparation, includes the following components in mass content: chlorfenapyr 10wt%, thiamethoxam 3.2wt%, emulsifier (Nongru 500 ) 12wt%, and the balance is solvent (N,N-dimethylformamide).

The preparation method of the pesticide preparation for preventing and treating tardigrade maggots described in this example is the same as that in Example 1.

Experimental example

The present invention carries out toxicity determination through indoor bioassay experiment, and the specific method adopts stomach poison contact method to screen out the compound ratio of chlorfenapyr and thiamethoxam, and the specific steps are as follows:

After dissolving the original drugs of chlorfenapyr and thiamethoxam in N,N-dimethylformamide, they were diluted with 0.1% TW-80% into 5 serial mass concentrations, and the filter paper was wetted with different mass concentrations of the drug solution, Place it in a petri dish with a diameter of 9 cm, cut the pseudostem of chives into 2 cm sections, and soak them in different concentrations of medicinal liquid for 15 seconds.

Take out 2 segments and place them in a petri dish covered with filter paper, then gently pick 20 third-instar larvae of the same size with a brush, and repeat each treatment 3 times, with clear water as the control. Put the petri dish in an incubator with a temperature of (25±1)°C and a relative humidity of (75±5)%, add appropriate amount of water to the incubator regularly to maintain the humidity, and add undipped leeks to the petri dish in time part.

The death of the test insects was observed 72 hours after the chemical treatment. The body wall of the larva was lightly touched with a brush but the larva was considered dead. The number of deaths was counted, and the death rate and corrected death rate were calculated.

The toxicity regression equation and LC ₅₀ value were determined by SPSS software, and the co-toxicity coefficient was calculated. According to the Ministry of Agriculture’s Pesticide Control Regulations, the judging criteria for synergistic effects of mixed formulations are: co-toxicity coefficient greater than 120 is synergistic effect, co-toxicity coefficient 80-120 is additive effect, and co-toxicity coefficient less than 80 is antagonistic effect. The selection of the optimal mixing ratio is measured by two indicators, the co-toxicity coefficient and the LC ₅₀ value, that is, the co-toxicity coefficient is higher and the LC ₅₀ value is the smallest. The results of the compound toxicity test are shown in Table 1 below.

Table 1 Compound toxicity assay results are shown in the following table:

Chlorfenapyr: Thiamethoxam LC ₅₀ co-toxicity coefficient Toxicity index theoretical virulence index Virulence regression equation 95% confidence limit R ² 1:0 5.58 —— 100 —— Y＝-1.739+0.312X 4.117～10.735 0.962 0:1 16.181 —— 34.485 —— Y＝-0.426+0.026X 3.454～31.251 0.975 1:26 16.724 90.393 33.365 36.911 Y＝-0.785+0.047X 12.949～20.936 0.962 1:12 14.506 97.324 38.467 39.525 Y=-1.661+1.430X 11.346～19.333 0.99 1:7 11.563 113.083 48.257 42.674 Y＝-1.543+1.451X 9.063～14.973 0.994 1:4 9.218 127.204 60.534 47.588 Y＝-1.321+1.369X 7.045-11.940 0.998 1:3 7.238 151.568 77.093 50.864 Y＝-1.318+0.182X 6.140～8.555 0.944 1:2 6.222 140.449 89.682 63.854 Y＝-0.825+1.039X 4.476～9.092 0.974 13:16 6.744 146.902 82.74 56.323 Y＝-0.865+1.044X 4.868～9.994 0.986 11:8 5.288 145.719 105.522 72.415 Y＝-0.735+1.016X 3.734～7.594 0.974 1:0.32 4.623 143.49 120.701 84.118 Y＝-1.130+0.244X 3.732～5.567 0.972

It can be seen from the above table 1 that the compounding ratio of chlorfenapyr and thiamethoxam is in the range of 1:0.32-26, and the co-toxicity coefficient is between 80-150, showing a synergistic additive effect; the compounding ratio of the two In the range of 1:0.32-4, its co-toxicity coefficient is greater than 120, showing obvious synergistic effect. Therefore, the combination of chlorfenapyr and thiamethoxam can effectively prevent and control chive maggots, and the synergistic effect is remarkable. It can achieve the purpose of improving the control effect and reducing the dosage of a single dose, and has strong development value.

Apparently, the above-mentioned embodiments are only examples for clear description, rather than limiting the implementation. For those of ordinary skill in the art, other changes or changes in different forms can be made on the basis of the above description. It is not necessary and impossible to exhaustively list all the implementation manners here. And the obvious changes or changes derived therefrom are still within the scope of protection of the present invention.

Claims (10)

1. it is a kind of prevent and treat Diptera subterranean pest-insect composition pesticide, it is characterised in that including mass ratio be 1：0.32-26 bromine Worm nitrile and Diacloden.

2. the composition pesticide of preventing and treating Diptera subterranean pest-insect according to claim 1, it is characterised in that the chlorfenapyr Mass ratio with Diacloden is 1：0.32-4.

3. the composition pesticide described in claim 1 or 2 is used for the purposes for preparing the pesticidal preparations of preventing and treating Diptera subterranean pest-insect.

4. a kind of pesticidal preparations for preventing and treating Diptera subterranean pest-insect, it is characterised in that the pesticidal preparations are with claim 1 or 2 Described composition pesticide is active ingredient, and in terms of the total amount of the pesticidal preparations, the mass content of the composition pesticide For 3.9-28.2wt%.

5. pesticidal preparations according to claim 4, it is characterised in that the pesticidal preparations also include emulsifying agent and solvent.

6. pesticidal preparations according to claim 5, it is characterised in that：

The emulsifying agent includes agriculture breast 500 and/or agriculture breast 1601；

The solvent includes acetone and/or N,N-dimethylformamide.

7. the pesticidal preparations according to claim 5 or 6, it is characterised in that in terms of the total amount of the pesticidal preparations, including such as The component of lower mass content：

Chlorfenapyr 0.7-10wt%；

Diacloden 3.2-18.2wt%；

Emulsifying agent 3-20wt%；

Balance of solvent.

8. pesticidal preparations according to claim 7, it is characterised in that in terms of the total amount of the pesticidal preparations, including it is as follows The component of mass content：

Chlorfenapyr 3-10wt%；

Diacloden 3.2-12wt%；

Emulsifying agent 3-20wt%；

Balance of solvent.

9. according to the pesticidal preparations described in claim any one of 4-8, it is characterised in that the Diptera subterranean pest-insect includes fragrant-flowered garlic Dish Bradysia fungus gnat.

A kind of 10. method for preparing any one of the claim 4-9 pesticidal preparations, it is characterised in that a selected amount of including taking The step of chlorfenapyr and the Diacloden active compound is mixed and dissolved.