CN107517995A - A kind of formula and preparation method and application for improving hybrid rice seeds storage capacity - Google Patents

Abstract

The invention belongs to the field of rice seeds and discloses a formula for improving the storage capacity of hybrid rice seeds, a preparation method and an application. The formula is made of the following raw materials in terms of mass percentage: dihydromyricetin extract 42.00-50.00%, resveratrol 4.00-8.00%, vitamin E 10.00-15.00%, vitamin C 10.00-15.00%, paclobutrazol 10.00% ~15.00%, agricultural streptomycin sulfate 10.00~15.00%. The raw materials are weighed according to the above ratio and placed in a blending tank, stirred evenly, packed in a composite aluminum bag or canned to obtain a formula for improving the storage capacity of hybrid rice seeds. The invention has reasonable formula, simple processing and convenient use, and provides an ideal medicine for improving the storage capacity of seeds.

Description

A formula for improving the storage capacity of hybrid rice seeds, its preparation method and application

technical field

The invention belongs to the field of rice seeds, in particular to a formula for improving the storage capacity of hybrid rice seeds, a preparation method and an application.

Background technique

Seed storage capacity refers to the average number of years that seeds can maintain their vitality under certain storage conditions, that is, the time it takes for seeds to lose their vitality from full maturity. Different from ordinary rice storage, the seed germination rate standard must still be met after storage to meet the needs of crop production. However, the seed vigor and germination rate of ordinary rice will decrease after storage, and the germination rate of hybrid rice seeds can drop below 70% after one year of storage, which cannot meet the production needs and be scrapped, causing huge losses. For the preservation of national strategic wasteland preparation seeds and the long-term storage of seed resources, the technical requirements for extending the storage capacity of seeds are even more urgent.

Since the storage resistance of seeds is determined by factors such as genetics, environmental conditions during seed development, and storage conditions, it is affected by seed quality, storage temperature, and seed moisture content. For a long time at home and abroad, the problem of shortening the storage capacity of seeds has been mainly solved by means of engineering technology, including the construction of low-temperature dry warehouses. On the basis of consuming a lot of manpower, material and financial resources, there are still a large number of seeds whose vigor has declined and are scrapped, affecting Safe storage of seeds and seed quality.

A large number of studies have shown that the decline in seed vigor during storage is mainly due to the damage of nucleic acid, membrane and protein synthesis systems in the seeds, the combined effects of membrane lipid peroxidation, chromosome and gene aberrations, and embryo protein degradation; Among them, the oxidation of unsaturated fatty acids in seeds will increase the permeability of cell membranes, and the free radicals and peroxides formed by oxidation will damage proteins, membrane structures, and cell tissues. The seed protection system can play a protective role by scavenging reactive oxygen species and limiting membrane lipid peroxidation. Studies in recent years have shown that proteins related to protein synthesis and metabolism will undergo drastic changes during storage in storage-intolerant seeds (Gao2016). In addition, resveratrol, an activator of histone deacetylase, can effectively delay aging (Yin Xianhui 2013). At the same time, a large number of studies have also shown that due to the spraying of gibberellin and the characteristics of the variety itself, the hybrid rice seeds still maintain active physiological metabolism during the later stage of seed maturity and storage, resulting in the consumption of some nutrients and increasing the concentration of harmful substances. Accumulation, after a period of storage of the harvested seeds, the seed vigor decreases rapidly, and the use of appropriate inhibitory substances can reduce and slow down this active physiological metabolism, reduce the resulting negative effects, and help the seeds to withstand storage ability to improve. In addition, the change of rice seed storage capacity is also related to factors such as seed moisture, temperature, humidity, and ventilation during storage. These effects can be avoided or alleviated through appropriate adjustments through artificial measures. Chemical substances such as light, O ₂ , CO ₂ , microorganisms, storage pests and fungicides used to kill pests and microorganisms will all affect the storage capacity of seeds.

Discussion on the technology of maintaining the storage ability of hybrid rice seeds, including packaging materials, sealed packaging, storage methods, fumigation treatment to prevent insects, and storage technology in cold storage, etc. are currently research hotspots in the field of seeds. But so far, there is no effective practical technology applied in production. Even under refrigerated conditions, the germination rate of seeds will decrease year by year, more than 3% per year, and the effect of seeds will be lost. Therefore, long-term preservation of resources and There is also the problem of loss of seed vigor in the country's stockpile of seeds for drought preparation. Therefore, prolonging the storage capacity of seeds through different ways is not only a very worthwhile question in biology, but also has great practical significance in agricultural production and national strategy.

Contents of the invention

In order to solve the shortcomings and deficiencies in the above-mentioned prior art, the primary purpose of the present invention is to provide a formula for improving the storage capacity of hybrid rice seeds. The formula can be used alone or in combination with field management measures in the later stage of rice seed maturation to improve the storage capacity of rice seeds.

Another object of the present invention is to provide a preparation method of the above formulation.

Another object of the present invention is to provide the application of the above formulation.

The purpose of the present invention is achieved through the following technical solutions:

A formula for improving the storage capacity of hybrid rice seeds, the formula is made of the following raw materials by mass percentage:

Preferably, the formula is made from the following raw materials by mass percentage:

The dihydromyricetin extract refers to a product obtained by extracting and purifying vine tea as a raw material, wherein the mass percentage of dihydromyricetin is ≥ 55%;

The resveratrol extract refers to a product obtained by extracting and purifying grape skins, grape seeds or knotweed as raw materials, wherein the mass percentage of resveratrol is ≥ 20%;

The mass percentage of vitamin E in the vitamin E is greater than 95%;

The mass percentage of vitamin C in the vitamin C is greater than 95%;

The paclobutrazol, calculated as paclobutrazol, has a mass percentage greater than 15%;

The mass percentage of streptomycin sulfate in the agricultural streptomycin sulfate is greater than 70%.

The dihydromyricetin extract is prepared according to the following steps: the fresh stems and leaves of vine tea are used to dry the raw materials of vine tea at a temperature of 35-45° C.; the dried raw materials of vine tea are extracted with ethanol to obtain vine tea Tea extract, concentrating the extract to 30-40% of its original volume, and placing the concentrate in a -4°C freezer for 24 hours, and drying the bottom of the liquid to precipitate to obtain the dihydromyricetin extract; the ethanol extraction In the method, the mass ratio of the dry raw material of vine tea to ethanol is 1:10-12, and the volume percentage concentration of ethanol is 85-95%; the vine tea produced in Guangdong, Guangxi, Hunan, Hubei or Jiangxi is used.

Preferably, the ethanol volume percentage concentration is 90%; the bottom sediment of the dried liquid is dried in an oven at 90-95°C; the vine tea is produced in spring and summer in Zhangjiajie, Hunan Vine tea.

The resveratrol extract is prepared according to the following steps: Polygonum cuspidatum is used as a raw material, and ethanol with a volume percentage of 6 times the volume of the raw material is 75% as an extraction solvent, and extracted for 1 hour at a temperature of 70-75°C. Concentrate and recover ethanol, and spray-dry the extract to obtain the resveratrol extract.

The vitamin E is produced by Zhengzhou Longsheng Food Chemical Co., Ltd.; the vitamin C is produced by Zhengzhou Chengwang Chemical Co., Ltd.;

The paclobutrazol is produced by Hubei Dixin Chemical Manufacturing Co., Ltd., and the mass percentage of paclobutrazol is greater than 15%; the agricultural streptomycin sulfate is produced by Sichuan Chengdu Puhui Biological Engineering Co., Ltd., and the mass percentage of streptomycin sulfate is greater than 15%. 70%.

The preparation method of the formula for improving the storage capacity of hybrid rice seeds includes the following steps: weighing dihydromyricetin extract, resveratrol extract, vitamin E, vitamin C, paclobutrazol and agricultural streptavidin sulfate according to the above ratio The ingredients are placed in a blending tank, stirred evenly, and packaged in a composite aluminum bag or canned to obtain a formula for improving the storage capacity of hybrid rice seeds.

Application of the above formula in improving the storage capacity of hybrid rice seeds.

The application comprises the following steps: in the early stage of hybrid rice seed production (approximately 12 days before harvest), the formula for improving the storage capacity of hybrid rice seeds is stirred and dissolved with water so as to form a 0.5-1.0% direct For the liquid, spray the liquid directly on the immature seeds in the hybrid rice seed production field according to the dosage of 50-60 kg/mu, and the storage capacity of the seeds can be significantly improved by spraying once.

Principle of the present invention is:

Dihydromyricetin is the main active ingredient in the vine tea plant, which has various functions such as anti-aging and anti-oxidation. The main function of dihydromyricetin in this formula is to protect the nucleic acid, membrane and protein synthesis system in the seed from damage during seed storage, reduce membrane lipid peroxidation, prevent the generation and accumulation of malondialdehyde, so as to achieve the improvement of hybrid rice seed storage capacity purposes.

Resveratrol: Resveratrol is a phytoncide, which has the functions of anti-cancer, lowering blood pressure, and preventing osteoporosis. At the same time, it has the effects of treating inflammation, reducing lipid metabolism disorders, and anti-oxidation. The use of this formula is mainly to reduce the side effects caused by gibberellin spraying in the process of hybrid rice seed production, so as to achieve the purpose of improving the storage capacity of hybrid rice seeds.

Vitamin E (Vitamin E) is a fat-soluble vitamin, and its hydrolyzed product is tocopherol, which is one of the most important antioxidants. The main purpose of this formula is to use it to easily enter the lipid cell membrane, participate in the lipid metabolism of embryo cells, and eliminate a series of free radicals in the metabolic process, thereby protecting embryo cells and improving the storage capacity of hybrid rice seeds.

Vitamin C: It is a strong water-soluble antioxidant. The use of this formula is mainly to use it to enhance the anti-stress ability and immunity of seed embryo cells to the external environment, reduce the side effects caused by gibberellin in the process of hybrid rice seed production, so as to achieve the purpose of improving the storage capacity of hybrid rice seeds . .

Paclobutrazol: It is a triazole plant growth regulator successfully developed in the 1980s and an inhibitor of endogenous gibberellin synthesis. The agricultural application value of paclobutrazol lies in its control effect on crop growth. It has the effects of delaying plant growth, inhibiting stem elongation, shortening internodes, promoting plant tillering, promoting flower bud differentiation, increasing plant stress resistance, and increasing yield. The use in this formula is mainly to use it to delay plant growth, hinder the synthesis and metabolism of seed germination substances, so as to achieve the purpose of improving the storage capacity of hybrid rice seeds.

Agricultural Streptomycin Sulfate: Easily soluble in water, it is an antibiotic fungicide. The main control objects are bacterial brown spot and bacterial rot, which are common agricultural medicines. The use in this formula is mainly to use its bactericidal effect of inhibiting protein synthesis to prevent microorganisms from damaging seed germs and achieve the purpose of improving the storage capacity of hybrid rice seeds.

Compared with the prior art, the present invention has the following advantages and beneficial effects:

(1) The formula of the present invention is reasonable, and comprehensively utilizes the respective effects of extracts in chemical raw materials and natural plants to improve seed storage capacity, and the effect of improving seed storage capacity is very obvious through experimental verification;

(2) The processing is simple and easy to use, which provides an ideal drug for improving the storage capacity of seeds.

detailed description

The present invention will be further described in detail below in conjunction with examples, but the embodiments of the present invention are not limited thereto.

The sources of raw materials used in the following examples are as follows:

1. The dihydromyricetin extract is prepared according to the following steps: use the fresh stems and leaves of vine tea, dry them at a temperature of 35 to 45°C to obtain the dry raw materials of vine tea; use the ethanol extraction method to obtain the vine tea dry raw materials Tea extract, concentrating the extract to 30-40% of its original volume, and placing the concentrate in a -4°C freezer for 24 hours, and drying the bottom of the liquid to precipitate to obtain the dihydromyricetin extract; the ethanol extraction In the method, the mass ratio of the dry raw material of rattan tea to ethanol is 1:10-12, and the volume percentage concentration of ethanol is 85-95%, preferably 90% volume percentage concentration. The vine tea adopts the vine tea produced in Guangdong, Guangxi, Hunan, Hubei, Jiangxi and other localities, preferably the vine tea produced in spring and summer in Zhangjiajie, Hunan. The bottom precipitation of the dried liquid is to dry the precipitation at the bottom of the concentrated liquid in an oven at 90-95°C. In the obtained dihydromyricetin extract product, the mass percentage of dihydromyricetin is ≥ 55%.

2. The resveratrol extract is prepared according to the following steps: take Polygonum cuspidatum rich in resveratrol as raw material, add 6 times the volume of the raw material and 75% ethanol as the extraction solvent, and use it at a temperature of 70-75 Extract at ℃ for 1 hour, concentrate and recover ethanol, and spray dry the extract to obtain resveratrol extract. In the resveratrol extract, the mass percentage of resveratrol is more than or equal to 20%.

3. Vitamin E is produced by Zhengzhou Longsheng Food Chemical Co., Ltd., and its mass percentage is greater than 95%.

4. Vitamin C is produced by Zhengzhou Chengwang Chemical Co., Ltd., and its mass percentage is greater than 95%.

5. Paclobutrazol: produced by Hubei Dixin Chemical Manufacturing Co., Ltd., calculated as paclobutrazol, the mass percentage is greater than 15%.

6. Agricultural streptomycin sulfate is produced by Sichuan Chengdu Puhui Biological Engineering Co., Ltd., and the mass percentage of streptomycin sulfate is 72%.

Example 1:

Weighing respectively 46.50% by mass percentage of dihydromyricetin extract, 7.50% by mass percentage of resveratrol, 11.00% by mass percentage of vitamin E, 12.00% by mass percentage of vitamin C, and 11.50% by mass percentage Paclobutrazol and 11.50% by mass of agricultural streptomycin sulfate are placed in a blending tank, stirred evenly, and packaged in a composite aluminum bag or canned to obtain a formula product for improving the storage capacity of hybrid rice seeds.

Example 2:

Weigh respectively 47.00% by mass percentage of dihydromyricetin extract, 7.00% by mass percentage of resveratrol, 11.50% by mass percentage of vitamin E, 11.50% by mass percentage of vitamin C, 11.50% by mass percentage Paclobutrazol and agricultural streptomycin sulfate with a mass percentage of 11.50% are placed in a blending tank, stirred evenly, and packaged in a composite aluminum bag or canned to obtain a formula product for improving the storage capacity of hybrid rice seeds.

Example 3: Changes in seed storage capacity of hybrid rice after being treated with formula product of the present invention

Weigh 500 g of the formula product prepared in Example 1, stir and dissolve it with 50 kg of water to make it into an emulsion, and fill it in a plastic bucket to obtain a 1% seed storage ability improvement formula product for direct use. The above-mentioned direct-use solution was used in four hybrid rice seed production fields of "Taifengyou 208", "Taifeng A", "Wufengyou 2118" and "Guangba Youhuazhan" (under natural growth conditions, rice wax ripening stage Spraying) Spray once, and the amount of spraying is 50kg/mu of direct liquid. During the experiment, a control CK without spraying direct-use liquid was set up at the same time.

After the seeds matured, the storage capacity of each series of hybrid rice seeds and control CK was measured by accelerated aging method.

The accelerated aging method of seeds is as follows: the above-mentioned sprayed and unsprayed control seeds were placed in a closed desiccator at 15°C and a relative humidity of 85% for 3 days, and then transferred to 42°C and a relative humidity of 85%. % in a closed desiccator for 8 days, and finally placed in a closed desiccator at 25°C and a relative humidity of 32% for 3 days, and the seed aging treatment ended. The above-mentioned seeds after aging treatment were respectively taken for germination rate experiment; the storage capacity of seeds was expressed by the germination rate of seeds after accelerated aging. The determination method of germination rate is carried out according to "Determination method of rice seed germination rate" (GB/T 3543).

It can be seen from Table 1 that after spraying the formula product once at the wax ripening stage, the storage capacity (germination rate) of rice seeds was significantly improved. The names of the above-mentioned hybrid rice combinations are provided by Guangdong Golden Rice Seed Industry Co., Ltd.

Table 1 Changes in seed storage capacity of hybrid rice seeds treated with formula products

Example 4: Changes in seed storage capacity of "998" series hybrid rice after being treated with the formula product of the present invention

Weigh 0.75 kg of the formulated product prepared in Example 2, stir and dissolve it with 100 kg of water to form an emulsion, and fill it in a plastic bucket to obtain a direct-use liquid of 0.75% by mass. Spray the above-mentioned direct-use solution in the four hybrid rice seed production fields of "Boyou 998", "Tianyou 998", Qiuyou 998" and "IIyou 998" once in the wax ripening stage. Each experimental field Area is 0.1 mu, and each spraying consumption is the direct liquid of 60kg/mu. After the seed is mature, by the method of accelerated aging, measure respectively the storage capacity change situation of each series of hybrid rice seed production experiment field seeds. During the experiment, simultaneously Set up control CK without spraying direct application solution.

After the seeds matured, the storage capacity of each series of hybrid rice seeds and control CK was measured by accelerated aging method.

The accelerated aging method of seeds is as follows: put the above-mentioned sprayed and unsprayed control seeds at the same time in a closed desiccator at 15°C and a relative humidity of 85% for 3 days, and then transfer to 42°C and a relative humidity of 85%. % in a closed desiccator for 8 days, and finally placed in a closed desiccator at 25°C and a relative humidity of 32% for 3 days, and the seed aging treatment ended. The above-mentioned seeds after aging treatment were respectively taken for germination rate experiment; the storage capacity of seeds was expressed by the germination rate of seeds after accelerated aging.

It can be seen from Table 2 that the storage capacity (germination rate) of 998” series hybrid rice seeds was significantly improved after spraying the formula product once at the wax ripening stage. The name of the above hybrid rice combination was provided by Guangdong Jindao Seed Industry Co., Ltd.

Table 2 Changes in seed storage capacity of "998" series hybrid rice seeds treated with formula products

The above-mentioned embodiment is a preferred embodiment of the present invention, but the embodiment of the present invention is not limited by the above-mentioned embodiment, and any other changes, modifications, substitutions, combinations, Simplifications should be equivalent replacement methods, and all are included in the protection scope of the present invention.

Claims (9)

1. a formula for improving hybrid rice seed storage capacity, is characterized in that: the formula is made from the following raw materials by mass percentage: 2. a kind of formula that improves hybrid rice seed storage ability according to claim 1 is characterized in that: the formula is made of the following raw materials by mass percentage: 3. a kind of formula that improves hybrid rice seed storage ability according to claim 1 is characterized in that: described dihydromyricetin extract refers to the product obtained by extraction and purification with vine tea as raw material, wherein The mass percentage content of dihydromyricetin ≥ 55%; The resveratrol extract refers to a product obtained by extracting and purifying grape skins, grape seeds or knotweed as raw materials, wherein the mass percentage of resveratrol is ≥ 20%; The mass percentage of vitamin E in the vitamin E is greater than 95%; The mass percentage of vitamin C in the vitamin C is greater than 95%; The paclobutrazol, calculated as paclobutrazol, has a mass percentage greater than 15%; The mass percentage of streptomycin sulfate in the agricultural streptomycin sulfate is greater than 70%. 4. a kind of prescription that improves hybrid rice seed storage ability according to claim 1 is characterized in that: described dihydromyricetin extract is prepared according to the following steps: adopt the fresh stem and leaf of vine tea, at temperature 35 After drying at ~45°C, the dried raw material of vine tea is obtained; the dried raw material of vine tea is extracted with ethanol to obtain the extract of vine tea, the extract is concentrated to 30-40% of its original volume, and the concentrated solution is placed at -4°C Stand in the cold storage for 24 hours, and the bottom of the dry liquid precipitates to obtain the dihydromyricetin extract; in the ethanol extraction method, the mass ratio of the dry raw material of rattan tea to ethanol is 1:10-12, and the volume percentage concentration of ethanol is 85-85. 95%; the rattan tea adopts the rattan tea produced in Guangdong, Guangxi, Hunan, Hubei or Jiangxi. 5. a kind of prescription that improves hybrid rice seed storage ability according to claim 4 is characterized in that: the volume percent concentration of described ethanol is 90%; Dry in an oven at ~95°C; the rattan tea is the rattan tea produced in spring and summer in Zhangjiajie, Hunan. 6. A formula for improving the storage capacity of hybrid rice seeds according to claim 1, characterized in that: the resveratrol extract is prepared according to the following steps: take Polygonum cuspidatum as raw material, and use 6 times the volume of raw material Ethanol with a volume percentage of 75% is used as an extraction solvent, extracted at a temperature of 70-75° C. for 1 hour, concentrated to recover the ethanol, and spray-dried the extract to obtain resveratrol extract. 7. the preparation method of a kind of prescription that improves hybrid rice seed storage capacity according to claim 1, is characterized in that comprising the following operation steps: take by weighing dihydromyricetin extract, resveratrol extract by above-mentioned proportioning , vitamin E, vitamin C, paclobutrazol and agricultural streptomycin sulfate are placed in a blending tank, stirred evenly, and packed in a composite aluminum bag or canned to obtain a formula for improving the storage capacity of hybrid rice seeds. 8. The application of a formula for improving the storage capacity of hybrid rice seeds according to claim 1 in improving the storage capacity of hybrid rice seeds is characterized in that: the application comprises the following steps: at the early stage of wax ripening of hybrid rice seeds , stirring and dissolving the formula for improving the storage capacity of hybrid rice seeds with water to form a direct-use liquid with a mass percentage of 0.5-1.0%, and spraying the direct-use liquid on the hybrid rice seed production field at an amount of 50-60 kg/mu On immature seeds, spraying once can significantly improve the storage capacity of seeds. 9. The application according to claim 8, characterized in that: the early stage of wax ripening of the hybrid rice seeds is 12 days before harvest.