CN107446978A - A kind of stem of noble dendrobium small-molecular peptides and preparation method thereof - Google Patents
A kind of stem of noble dendrobium small-molecular peptides and preparation method thereof Download PDFInfo
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- 241000196324 Embryophyta Species 0.000 claims abstract description 22
- 108091005804 Peptidases Proteins 0.000 claims abstract description 20
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 20
- 229940108461 rennet Drugs 0.000 claims abstract description 19
- 108010058314 rennet Proteins 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 16
- 108010059892 Cellulase Proteins 0.000 claims abstract description 13
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- 239000007788 liquid Substances 0.000 claims description 12
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- 238000001035 drying Methods 0.000 claims description 10
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- 235000019834 papain Nutrition 0.000 claims description 10
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- 239000000919 ceramic Substances 0.000 claims description 8
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- 239000012141 concentrate Substances 0.000 claims description 7
- ZHQQRIUYLMXDPP-SSDOTTSWSA-N Actinidine Natural products C1=NC=C(C)C2=C1[C@H](C)CC2 ZHQQRIUYLMXDPP-SSDOTTSWSA-N 0.000 claims description 6
- 108090000350 actinidain Proteins 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 6
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- 238000004108 freeze drying Methods 0.000 claims description 2
- 238000005374 membrane filtration Methods 0.000 claims 2
- 241000282553 Macaca Species 0.000 claims 1
- 240000006413 Prunus persica var. persica Species 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 9
- 150000001875 compounds Chemical class 0.000 abstract description 8
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- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000010183 spectrum analysis Methods 0.000 description 3
- 229930013930 alkaloid Natural products 0.000 description 2
- 150000003797 alkaloid derivatives Chemical class 0.000 description 2
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- 238000000502 dialysis Methods 0.000 description 1
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
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- 239000003205 fragrance Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
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- 238000004255 ion exchange chromatography Methods 0.000 description 1
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- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 229940034610 toothpaste Drugs 0.000 description 1
- 239000000606 toothpaste Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention provides the preparation method of stem of noble dendrobium small-molecular peptides and thus obtained stem of noble dendrobium small-molecular peptides, the preparation method to comprise the following steps：1) pre-process；2) extract；3) digest；4) isolate and purify；5) it is concentrated and dried.The present invention is carried out using zytase, cellulase, alkali protease and plant rennet by the way of compound bio digestion, so that the time of enzymolysis foreshortens to 23 hours, and due to pH value need not be adjusted in enzymolysis, save the process time, the technique for eliminating desalination simultaneously, improve the production efficiency of stem of noble dendrobium small-molecular peptides.The stem of noble dendrobium small-molecular peptides molecular weight obtained by the preparation method is concentrated mainly on 100 500Da, is easily absorbed by the human body, can be applied to field of food.
Description
Technical field
The invention belongs to biology and new medical technology field, is related to one kind using the stem of noble dendrobium as raw material, using compound biological enzyme
Cut and bio-chemistry separation technique, the preparation method of extraction stem of noble dendrobium small-molecular peptides.Moreover, it relates to it is prepared into by the above method
The stem of noble dendrobium small-molecular peptides arrived and its application.
Background technology
The stem of noble dendrobium is the traditional rare Chinese medicine in China, and top grade is classified as in " Sheng Nong's herbal classic ".Stem of noble dendrobium slightly sweet flavor is micro- salty,
It is cold, return stomach, kidney, lung channel.Reinforcing stomach reg fluid, nourishing Yin and clearing heat.
The stem of noble dendrobium mainly contains the active ingredients such as dendrobium polysaccharide, alkaloid, amino acid, trace element and luxuriant and rich with fragrance class compound, wherein
The nearly half of amino acid is essential amino acid, also contains soluble protein.Modern study shows that there is enhancing body to exempt from for it
Epidemic disease power, antitumor, promotion digestive juice secretion, suppress platelet aggregation, reducing blood lipid, hypoglycemic, anti-oxidant, anti-aging and bring down a fever only
The pharmacological actions such as pain.
It is reported that before 2001-2011, the domestic research on the stem of noble dendrobium, it is concentrated mainly on the pharmacology of its alkaloid, contains
Amount etc., 2004 so far, is concentrated mainly on pharmacology, optimal extraction technology of polysaccharide etc., such as a kind of to improve the stem of noble dendrobium
The biology enzyme assisted extraction method (CN201210472158.X) of bioactive polysaccharide dissolution rate；And the other compositions of the stem of noble dendrobium, especially
It is that peptide aspect research is then less.
But, there are some researchs in terms of stem of noble dendrobium peptide in recent years gradually into focus.Such as, using the stone of papain
The preparation method (CN201610214799.3) of dry measure used in former times peptide, using 3 kinds of protease dendrobium candidum antineoplastic polypeptide preparation method
And application (CN201410466768.8), small using a kind of dendrobium candidum based on nanometer gold bar compound material of single protease
Molecular peptide toothpaste (CN201610207244.6) and the neutral dendrobium candidum small-molecular peptides group with acid protease of use alkalescence
Its extracting method of compound and application (CN201610192437.9).
In above-mentioned patented technology, often product quality is bad for peptide prepared by described single enzyme, is come using a variety of enzymes
Enzymolysis, cost is higher and the process time greatly increases, and post-processing step is cumbersome, is unfavorable for mass production.Therefore, it is necessary to open
Send out new technology a kind of, it is ensured that high quality, be expeditiously applied to production stem of noble dendrobium small-molecular peptides product.
The content of the invention
In order to solve the above-mentioned technical problem, on the premise of quality is ensured, shorten enzymolysis time, simplify integrated artistic stream
Journey, production efficiency is improved, the invention provides a kind of preparation method of stem of noble dendrobium small-molecular peptides and small point of the thus obtained stem of noble dendrobium
Sub- peptide.
The step of stem of noble dendrobium small-molecular peptides preparation method of the present invention is：
1) pre-process：The fresh stem of noble dendrobium is crushed into defibrination into slurries；
2) extract：The slurries that will be obtained in step 1), addition quality are the water of 3-5 times of grind slurries quality, are heated up to 40-80
DEG C, ultrasonic extraction 10-30 minutes obtain extract solution；
3) digest：The extract solution obtained in step 2) is heated up to 50-55 DEG C, composition A and composition B is added and carries out enzyme
Solution, wherein composition A are made up of zytase and cellulase, and composition B is made up of alkali protease and plant rennet；
4) isolate and purify：By the enzymolysis liquid obtained in step 3), sterilized at 85-105 DEG C, destroy the enzyme treatment 10-15
Minute, stand, isolate and purify to obtain supernatant；
5) it is concentrated and dried：Obtained supernatant is isolated and purified by above-mentioned, is concentrated and dried and obtains stem of noble dendrobium small-molecular peptides.
According to the embodiment of the present invention, in step 3), the quality of zytase can be the extraction in composition A
The 0.08%-0.15% of liquid quality, the quality of cellulase can be the 0.01%-0.05% of the extract solution quality；Combination
The quality of thing B neutral and alkali protease can be the 0.1%-0.2% of the extract solution quality, and the quality of plant rennet can be
The 0.1%-0.25% of the extract solution quality；The plant rennet is by papain, bromelain and Kiwi berry egg
White enzyme is formed, and the mass ratio of three kinds of enzymes is 4:3:3.
According to the embodiment of the present invention, in step 3), composition A enzymolysis 0.5-1 hours can be first added, add group
Compound B digests 1.5-2.5 hours.
According to the embodiment of the present invention, in step 3), the mixed of composition A and composition B can be added into extract solution
Compound, digest 2-3 hours.
According to the embodiment of the present invention, the method isolated and purified described in step 4) is filter, centrifuge or film mistake
One or more in filter, it is preferable that the method isolated and purified is through ceramic super-filtering film after carrying out coarse filtration with twin strainer
Filtering.
According to the embodiment of the present invention, it is concentrated and dried described in step 5) as spray drying, vacuum belt type drying, vacuum
One or more in microwave drying, freeze-drying.
According to the embodiment of the present invention, the stem of noble dendrobium that uses of the present invention can be but not limited to HERBA DENDROBII, dendrobium candidum,
Purple dendrobium.
According to another aspect of the present invention, the present invention provides the stem of noble dendrobium small-molecular peptides obtained according to the preparation method.
In accordance with a further aspect of the present invention, the present invention provides the stem of noble dendrobium small-molecular peptides of present invention gained answering in food
With.
By implementing above technical scheme, present invention contrast prior art has such as the following advantage：
1. shorten enzymolysis time：By compounded enzyme preparate and bio-chemistry separation technique, regulation enzymolysis parameter, enzyme is greatlyd save
The time is solved, 9-12 hours are needed in existing literature, and this technology only needs the enzymolysis of 2-3 hours, the time is shorter, and enzymolysis is then more thorough
Bottom.
2. simplify processing step：The present invention uses zytase and cellulase, the plant of partial neutral of the appropriate pH for acidity
Thing protease and alkali protease, therefore the pH accommodations of complex enzyme are wide, directly dendrobium extract can be digested, nothing
PH value need to be adjusted, saves the process time, while reduces the generation of ash content and salinity among production technology, is eliminated in production
The production technology link of the expensive low yield energy such as the technique of desalination, dialysis cartridge, ion-exchange chromatography.
3. energy-saving and emission-reduction：The present invention directly can carry out separating and filtering using ceramic membrane filter to it, described in document
Diatomite filtering compares, and reduces because diatomite brings microbial safety risk in production, at the same ceramic membrane be readily cleaned and
Regeneration, and diatomite is disposable brings substantial amounts of waste water and discarded object, ensures that energy-saving and emission-reduction.
4. product has nutritive value：The present invention provides stem of noble dendrobium small-molecular peptides composition, and its molecular weight is mainly distributed on
Below 1000Da, these peptides are small-molecular peptides, are easily absorbed by the body, and have important nutritive value.
Embodiment
With reference to embodiment, embodiment of the invention is more fully described.Following examples are merely to illustrate
Purpose and do not limit the scope of the invention.
Raw material, instrument and equipment and reagent used in the present invention have that the product of commercialization is alternative, and can be from the market
It is commercially available.
Embodiment 1
The preparation method of stem of noble dendrobium small-molecular peptides be by the stem of noble dendrobium it is preprocessed, extract, digest, isolate and purify and be concentrated and dried
It is refined, obtain stem of noble dendrobium small-molecular peptides product.
1) fresh HERBA DENDROBII is obtained into slurries by crushing defibrination；
2) water that quality is 5 times of grind slurries quality is added, is heated up to 40 DEG C, ultrasonic extraction 30 minutes, ultrasonic power 30kW,
Obtain extract solution；
3) extract solution is warmed to 50 DEG C, first added and zytase that the mass ratio of extract solution is 0.08%, 0.01%
Cellulase degradation react 1 hour, alkali protease, 0.1% plant that the mass ratio of rear addition and extract solution is 0.1%
Protease carries out secondary enzymolysis, and enzymolysis time is 2 hours；The plant rennet is by papain, bromelain and Mi
Monkey peach protease is formed, and the mass ratio of three kinds of enzymes is 4:3:3.
4) enzymolysis liquid is warming up into 85 DEG C to keep carrying out sterilization enzyme inactivation work processing in 15 minutes, passes through sheet frame after standing 30 minutes
Filter carries out isolated supernatant；
5) supernatant is dried by vacuum microwave drier to obtain finished product.
After measured, the product oligomeric composition is in taupe, and the micro- sweet tea of mouthfeel is slightly puckery, water content 2.8%, passes through liquid phase color
Spectrum analysis, product middle-molecular-weihydroxyethyl account for 91% for 100-500Da's.
The stem of noble dendrobium small-molecular peptides obtained can be applied to field of food.
Embodiment 2
The preparation method of stem of noble dendrobium small-molecular peptides be by HERBA DENDROBII it is preprocessed, extract, digest, isolate and purify and concentrate
Drying is refining to obtain stem of noble dendrobium small-molecular peptides product.
1) fresh HERBA DENDROBII is obtained into slurries by crushing defibrination；
2) water that quality is 3 times of grind slurries quality is added, is heated up to 60 DEG C, ultrasonic extraction 20 minutes, ultrasonic power 30kW,
Obtain extract solution；
3) extract solution is warmed to 52 DEG C, first added and zytase that the mass ratio of extract solution is 0.1%, 0.02%
Cellulase degradation reacts 0.5 hour, rear to add and alkali protease of the mass ratio of extract solution for 0.15%, 0.15% plant
Thing protease carries out secondary enzymolysis, and enzymolysis time is 2.5 hours；The plant rennet is by papain, bromelain
Formed with Actinidin, the mass ratio of three kinds of enzymes is 4:3:3.
4) enzymolysis liquid is warming up into 85 DEG C to keep carrying out sterilization enzyme inactivation work processing in 15 minutes, passes through flat board hanging bag type centrifugal machine
Carry out isolated supernatant；
5) supernatant is dried by vacuum belt drier to obtain finished product.
After measured, the product oligomeric composition is in taupe, and the micro- sweet tea of mouthfeel is slightly puckery, water content 4.6%, passes through liquid phase color
Spectrum analysis, product middle-molecular-weihydroxyethyl account for 90% for 100-500Da's.
The stem of noble dendrobium small-molecular peptides obtained can be applied to field of food.
Embodiment 3
The preparation method of stem of noble dendrobium small-molecular peptides be by HERBA DENDROBII it is preprocessed, extract, digest, isolate and purify and concentrate
Drying is refining to obtain stem of noble dendrobium small-molecular peptides product.
1) fresh HERBA DENDROBII is obtained into slurries by crushing defibrination；
2) water that quality is 4 times of grind slurries quality is added, is heated up to 80 DEG C, ultrasonic extraction 10 minutes, ultrasonic power 30kW,
Obtain extract solution；
3) extract solution is warmed to 55 DEG C, first added and zytase that the mass ratio of extract solution is 0.12%, 0.05%
Cellulase degradation react 0.5 hour, alkali protease that the mass ratio of rear addition and extract solution is 0.2%, 0.25%
Plant rennet carries out secondary enzymolysis, and enzymolysis time is 1.5 hours；The plant rennet is by papain, bromelain
Enzyme and Actinidin are formed, and the mass ratio of three kinds of enzymes is 4:3:3.
4) after enzymolysis liquid being warming up into 102 DEG C of holdings progress sterilization enzyme inactivation work processing in 10 minutes, carried out by twin strainer
After coarse filtration supernatant is filtrated to get through ceramic super-filtering film；
5) supernatant is dried to obtain finished product by high-speed centrifugal spraying.
After measured, the product oligomeric composition is in dark brown, and the micro- sweet tea of mouthfeel is slightly puckery, water content 3%, passes through liquid chromatogram
Analysis, product middle-molecular-weihydroxyethyl account for 93% for 100-500Da's.
The stem of noble dendrobium small-molecular peptides obtained can be applied to field of food.
Embodiment 4
The preparation method of stem of noble dendrobium small-molecular peptides be by HERBA DENDROBII it is preprocessed, extract, digest, isolate and purify and concentrate
Drying is refining to obtain stem of noble dendrobium small-molecular peptides product.
1) fresh HERBA DENDROBII is obtained into slurries by crushing defibrination；
2) water that quality is 3 times of grind slurries quality is added, is heated up to 70 DEG C, ultrasonic extraction 10 minutes, ultrasonic power 30kW,
Obtain extract solution；
3) extract solution is warmed to 55 DEG C, added and zytase that the mass ratio of extract solution is 0.08%, 0.01%
Cellulase, 0.1% alkali protease and 0.1% plant rennet, enzyme digestion reaction 3 hours；The plant rennet
It is made up of papain, bromelain and Actinidin, the mass ratio of three kinds of enzymes is 4:3:3.
4) after enzymolysis liquid being warming up into 102 DEG C of holdings progress sterilization enzyme inactivation work processing in 10 minutes, carried out by twin strainer
After coarse filtration supernatant is filtrated to get through ceramic super-filtering film；
5) supernatant is dried to obtain finished product by high-speed centrifugal spraying.
After measured, the product oligomeric composition is in dark brown, and the micro- sweet tea of mouthfeel is slightly puckery, water content 3%, passes through liquid chromatogram
Analysis, product middle-molecular-weihydroxyethyl account for 92% for 100-500Da's.
The stem of noble dendrobium small-molecular peptides obtained can be applied to field of food.
Embodiment 5
The preparation method of stem of noble dendrobium small-molecular peptides be by HERBA DENDROBII it is preprocessed, extract, digest, isolate and purify and concentrate
Drying is refining to obtain stem of noble dendrobium small-molecular peptides product.
1) fresh HERBA DENDROBII is obtained into slurries by crushing defibrination；
2) water that quality is 4 times of grind slurries quality is added, is heated up to 65 DEG C, ultrasonic extraction 15 minutes, ultrasonic power 30kW,
Obtain extract solution；
3) extract solution is warmed to 55 DEG C, added and zytase that the mass ratio of extract solution is 0.1%, 0.025%
Cellulase, 0.15% alkali protease, 0.15% plant rennet enzyme digestion reaction 2 hours；The plant rennet by
Papain, bromelain and Actinidin are formed, and the mass ratio of three kinds of enzymes is 4:3:3.
4) after enzymolysis liquid being warming up into 102 DEG C of holdings progress sterilization enzyme inactivation work processing in 10 minutes, carried out by twin strainer
After coarse filtration supernatant is filtrated to get through ceramic super-filtering film；
5) supernatant is dried to obtain finished product by high-speed centrifugal spraying.
After measured, the product oligomeric composition is in dark brown, and the micro- sweet tea of mouthfeel is slightly puckery, water content 3%, passes through liquid chromatogram
Analysis, product middle-molecular-weihydroxyethyl account for 92% for 100-500Da's.
The stem of noble dendrobium small-molecular peptides obtained can be applied to field of food.
Embodiment 6
The preparation method of stem of noble dendrobium small-molecular peptides is that purple dendrobium, dendrobium candidum are distinguished into preprocessed, extraction, enzymolysis, divided
Stem of noble dendrobium small-molecular peptides product is refining to obtain from purifying and concentrate drying.
1) fresh purple dendrobium, dendrobium candidum are obtained into slurries respectively by crushing defibrination；
2) water that quality is 5 times of grind slurries quality is added, is heated up to 65 DEG C, ultrasonic extraction 15 minutes, ultrasonic power 30kW,
Obtain extract solution；
3) extract solution is warmed to 55 DEG C, added and zytase that the mass ratio of extract solution is 0.15%, 0.05%
Cellulase, 0.2% alkali protease, 0.25% plant rennet, enzyme digestion reaction 3 hours；The plant rennet by
Papain, bromelain and Actinidin are formed, and the mass ratio of three kinds of enzymes is 4:3:3.
4) after enzymolysis liquid being warming up into 105 DEG C of holdings progress sterilization enzyme inactivation work processing in 10 minutes,
By being filtrated to get supernatant through ceramic super-filtering film after twin strainer progress coarse filtration.
5) supernatant is dried to obtain finished product by high-speed centrifugal spraying.
After measured, purple dendrobium oligomeric composition is in bluish violet, and the micro- sweet tea of mouthfeel is slightly puckery, water content 4.6%, molecule quantity set
In in below 1000Da；Dendrobium candidum oligomeric composition gray, mouthfeel is slightly puckery compared with sweet tea, water content 3.8%, passes through liquid phase color
Spectrum analysis, product middle-molecular-weihydroxyethyl account for 93% for 100-500Da's.
The stem of noble dendrobium small-molecular peptides obtained can be applied to field of food.
Embodiment 1-6 is analyzed, obtains the technological parameter and product of stem of noble dendrobium small-molecular peptides preparation, as shown in table 1.
Table 1
As seen from the above table, compound bio is carried out by zytase, cellulase, alkali protease and plant rennet
Digestion, the time of enzymolysis can foreshorten to 2-3 hours, and need not adjust pH value during enzymolysis, save the process time, reduce simultaneously
The generation of ash content and salinity among production technology, the technique for eliminating desalination.The molecular weight of product finally obtained concentrates on 100-
500Da, it is easily absorbed by the human body.
So far, although those skilled in the art will appreciate that detailed herein have shown and described multiple showing for the present invention
Example property embodiment, still, still can be direct according to present disclosure without departing from the spirit and scope of the present invention
It is determined that or derive many other variations or modifications for meeting the principle of the invention.
Claims (10)
1. a kind of preparation method of stem of noble dendrobium small-molecular peptides, its step are：
1) pre-process：The fresh stem of noble dendrobium is crushed into defibrination into slurries；
2) extract：The slurries that will be obtained in step 1), addition quality are the water of 3-5 times of grind slurries quality, are heated up to 40-80 DEG C, surpass
Sound extraction 10-30 minutes obtain extract solution；
3) digest：The extract solution obtained in step 2) is heated up to 50-55 DEG C, composition A and composition B is added and is digested,
Wherein composition A is made up of zytase and cellulase, and composition B is made up of alkali protease and plant rennet；It is described
Plant rennet is made up of papain, bromelain and Actinidin；
4) isolate and purify：By the enzymolysis liquid obtained in step 3), sterilized at 85-105 DEG C, destroy the enzyme treatment 10-15 minutes,
Stand, isolate and purify to obtain supernatant；
5) it is concentrated and dried：Obtained supernatant is isolated and purified by above-mentioned, is concentrated and dried and obtains stem of noble dendrobium small-molecular peptides.
2. preparation method according to claim 1, it is characterised in that in step 3), the matter of zytase in composition A
It is the 0.01%- of the extract solution quality to measure as the 0.08%-0.15% of the extract solution quality, the quality of cellulase
0.05%；The quality of composition B neutral and alkali protease be the extract solution quality 0.1%-0.2%, the matter of plant rennet
Measure as the 0.1%-0.25% of the extract solution quality；In the plant rennet, papain, bromelain and macaque
The mass ratio of peach protease is 4:3:3.
3. preparation method according to claim 2, it is characterised in that in step 3), first add composition A enzymolysis 0.5-
1 hour, add composition B enzymolysis 1.5-2.5 hours.
4. preparation method according to claim 2, it is characterised in that in step 3), composition A is added into extract solution
With composition B mixture, 2-3 hours are digested.
5. preparation method according to claim 1, it is characterised in that the method isolated and purified described in step 4) is filtering
One or more in device, centrifuge or membrane filtration.
6. preparation method according to claim 5, it is characterised in that the method isolated and purified is to be entered with twin strainer
After row coarse filtration, through Ceramic excessive filtration membrane filtration.
7. preparation method according to claim 1, it is characterised in that the concentrate drying described in step 5) is dry for spraying
One or more in dry, vacuum belt type drying, vacuum microwave drying or freeze-drying.
8. according to the preparation method described in claim any one of 1-7, it is characterised in that the stem of noble dendrobium described in step 1) is Jin Chai
The stem of noble dendrobium, dendrobium candidum or purple dendrobium.
9. a kind of stem of noble dendrobium small-molecular peptides, its preparation method according to any one of claim 1-8 obtains.
10. application of the stem of noble dendrobium small-molecular peptides according to claim 9 in food.
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