CN102191306B - Enzymatic preparation method for antihypertensive peptides from jellyfish - Google Patents
Enzymatic preparation method for antihypertensive peptides from jellyfish Download PDFInfo
- Publication number
- CN102191306B CN102191306B CN2011101002208A CN201110100220A CN102191306B CN 102191306 B CN102191306 B CN 102191306B CN 2011101002208 A CN2011101002208 A CN 2011101002208A CN 201110100220 A CN201110100220 A CN 201110100220A CN 102191306 B CN102191306 B CN 102191306B
- Authority
- CN
- China
- Prior art keywords
- jellyfish
- enzyme
- temperature
- controlled
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
本发明涉及一种海蜇降压肽的酶法制备方法。其是选取新鲜海蜇或矾制海蜇为原料，经脱盐、洗净后进行研磨处理；调节海蜇浆的蛋白质浓度为1％～3％；调整pH值为2～3.5，控温35～40℃，加入胃蛋白酶保温酶解4～6h，胃蛋白酶的用量为每克蛋白质加入2000～4000u；再将海蜇胃蛋白酶水解液调整pH值为5～6，控温50～55℃，加入木瓜蛋白酶酶解1～3h，木瓜蛋白酶用量为每克蛋白质加入2000～4000u；酶灭活；再经膜过滤除杂、纳滤膜脱盐、浓缩、喷雾干燥或冷冻干燥得到海蜇降压肽粉。本发明制备的海蜇降压肽经SHR大鼠(原发性高血压大鼠)口服实验证明具有较好的辅助降血压功效。本发明生产工艺先进，可操作性强，蛋白质酶解完全，产品质量可控。The invention relates to an enzymatic preparation method of jellyfish antihypertensive peptide. It selects fresh jellyfish or alum-made jellyfish as raw materials, grinds them after desalination and washing; adjusts the protein concentration of the jellyfish pulp to 1% to 3%; adjusts the pH value to 2 to 3.5, and controls the temperature to 35 to 40°C. Add pepsin to keep warm for 4-6 hours, and the dosage of pepsin is 2000-4000u per gram of protein; then adjust the pH value of jellyfish pepsin hydrolyzate to 5-6, control the temperature at 50-55°C, and add papain for enzymolysis 1 to 3 hours, the dosage of papain is 2000 to 4000u per gram of protein; the enzyme is inactivated; then the jellyfish antihypertensive peptide powder is obtained through membrane filtration to remove impurities, nanofiltration membrane desalination, concentration, spray drying or freeze drying. The jellyfish antihypertensive peptide prepared by the invention is proved to have good auxiliary effect of lowering blood pressure through oral experiments on SHR rats (essentially hypertensive rats). The invention has advanced production technology, strong operability, complete protein enzymolysis and controllable product quality.
Description
Technical field
The present invention relates to a kind of general preparation method of peptide, especially a kind ofly by enzymatic hydrolysis, obtain jellyfish collagen peptide liquid, then through the enzymatic-process preparation method that separates, concentrated, drying obtains the jellyfish Antihypertensive Peptides of jellyfish step-down Gly-His-Lys.
Background technology
Jellyfish, claim again jellyfish, is the enterozoa of Hai Sheng, and not only kind is many for they, and quantity is large, and it is wide to distribute, and is the important component part of marine zooplankton group.Jellyfish vegetal pole horn of plenty, its body contain the inorganic salt such as higher protein and calcium, phosphorus, iodine, iron, have the laudatory title of " aquatic products rarity ".Jellyfish is not only edible, but also is the good medicine of curing the disease simply.Described in Compendium of Material Medica: jellyfish has the functions such as clearing heat and detoxicating, resolving phlegm and softening hard masses, step-down detumescence, and is all effective in cure to diseases such as trachitis, asthma, hypertension, stomach ulcer.Therefore, jellyfish is as a kind of macro-organism of curing the food homology, and it has important economy and edibleness.
The method for preparing bioactive peptide mainly contains: directly extraction, external degradation and utilize recombinant DNA technology, enzyme process or chemical method composite reactive peptide.Extracorporeal hydrolysis protein preparation bioactive peptide has three operational paths: acid hydrolysis, basic hydrolysis and enzymatic hydrolysis.The former two's method is simple, but has the deleterious effect of nutrition and toxicity aspect, and therefore the application in food-processing is greatly limited.The enzymatic hydrolysis of protein generally can not cause the loss of nutrition aspect, also can not produce the problem on toxicity; Enzyme effect simultaneously has specificity, can carry out under the condition of gentleness, and energy consumption is also low, so enzymatic hydrolysis often application in food-processing.
and with the technology of enzymatic hydrolysis jellyfish protein preparation jellyfish bioactive peptide, have been reported, for example: by the applicant application and on May 6th, 2009 disclosed CN101422195A Chinese invention patent application Publication Specification disclosed " a kind of jellyfish active peptide yoghourt and preparation method thereof ", in on May 6th, 2009 disclosed CN101422266A Chinese invention patent application Publication Specification disclosed " a kind of jelly-fish nutrient liquid and preparation method thereof ", in on March 17th, 2010 disclosed CN101669659A Chinese invention patent application Publication Specification disclosed " a kind of jellyfish water chestnut drink and preparation method thereof ", in on October 20th, 2010 disclosed CN101862375A Chinese invention patent application Publication Specification disclosed " a kind of compound jellyfish soluble granules for reducing blood pressure and preparation method thereof ", in above-mentioned open file to the jellyfish raw material through desalination, clean, broken, after thin up or adopt Sumizyme MP or adopt trypsinase to carry out enzymolysis and obtain jellyfish polypeptide liquid, then for the preparation of other functional products.In disclosed technology, what jellyfish was carried out that enzymolysis all adopts is single enzyme.And in proteolysis, the selection of enzyme is gordian technique, and the difference of enzyme used and separation purification method can obtain different products, and the activity of the peptide product of different enzymolysis process gained is different.At present, not yet see the report that adopts dual-enzyme hydrolysis method to prepare the jellyfish Antihypertensive Peptides.
Summary of the invention
, in order to overcome deficiency of the prior art, the invention provides the enzymatic-process preparation method of the jellyfish Antihypertensive Peptides that a kind of production technique is advanced, quality product is controlled, protein digestion is complete, the jellyfish peptide of gained activity is high.
The technical solution adopted for the present invention to solve the technical problems is: a kind of enzymatic-process preparation method of jellyfish Antihypertensive Peptides is characterized in that: through the following step
(a) processing of raw material is chosen fresh jellyfish or alum jellyfish processed is raw material, and clear water soaks desalination to salinity＜5%, adopts colloidal mill to carry out milled processed after cleaning, and gained jellyfish slurry particle fineness is controlled at 2～5um;
(b) protein content of the first step enzymolysis determination step (a) gained jellyfish slurry, the mass concentration that adds water adjustment protein is 1%～3%; Adjusting the pH value is 2～3.5, and controlling temperature is 35～40 ℃, adds stomach en-, and pepsic consumption is the stomach en-that every gram protein adds 2000～4000u, and insulation enzymolysis 4～6h, obtain jellyfish pepsin hydrolysis liquid;
(c) the second step enzymolysis is 5～6 with the jellyfish pepsin hydrolysis liquid adjustment pH value that step (b) obtains, controlling temperature is 50～55 ℃, add papoid, the papoid consumption is the papoid that every gram protein adds 2000～4000u, enzymolysis 1～3h, obtain the two enzyme enzymolysis solutions of jellyfish;
(d) enzyme-deactivating is heated to 90～100 ℃ with the two enzyme enzymolysis solutions of jellyfish that step (c) obtains, and keeps 10～20 minutes, carries out enzyme-deactivating, the two enzyme enzymolysis solutions of the enzyme jellyfish of must going out;
(e) impurity elimination, desalination are removed impurity with the two enzyme enzymolysis solutions of the enzyme jellyfish of going out that step (d) obtains through tubular membrane, then adopt the nanofiltration membrane desalination of 150～300 trapped molecular weights, and concentrate, obtain jellyfish Antihypertensive Peptides concentrated solution, the soluble solid content of this jellyfish Antihypertensive Peptides concentrated solution is 15%～20%;
(f) the dry jellyfish Antihypertensive Peptides concentrated solution that step (e) is obtained carries out spraying drying or lyophilize, obtains jellyfish step-down Gly-His-Lys.
The two enzyme enzymolysis solutions of described jellyfish after enzyme liquid is gone out go deimpurity tubular membrane, and its work aperture is 0.1～0.2 μ m, and working temperature is controlled at 30～40 ℃, advances film pressure and is controlled at 2.5～3bar, and the membrane pressure-controlling is at 0.8～1.0bar.
The described spraying drying that jellyfish Antihypertensive Peptides concentrated solution is carried out, its inlet temperature are controlled at 185 ℃～190 ℃, and air outlet temperature is controlled at 75～80 ℃, and spray pressure is 0.4MPa.
The described lyophilize that jellyfish Antihypertensive Peptides concentrated solution is carried out, its vacuum degree control are at 50～133Pa, and temperature of heating plate: 20 ℃ 250min/40 ℃ 400min/30 ℃ until the freeze-dried material constant weight;
Condenser temperature is controlled at-40 ℃～-50 ℃.
The present invention is take bright jellyfish or alum jellyfish processed as raw material, after desalination, grinding, obtain jellyfish proteolysis peptide liquid with stomach en-and papoid substep enzymolysis respectively, then separate and obtain jellyfish Antihypertensive Peptides liquid by membrane filtration removal of impurities, nanofiltration membrane desalination, then through concentrated, spraying drying or lyophilize, obtain jellyfish step-down Gly-His-Lys.The jellyfish Antihypertensive Peptides of the present invention's preparation has auxiliary blood pressure reduction effect preferably through oral experimental results show that of SHR rat (original hypertensive rat).The production technique of this jellyfish Antihypertensive Peptides is advanced, and workable, quality product is controlled, and protein digestion is complete, and the jellyfish step-down Gly-His-Lys of gained is white in color or is faint yellow, and is active high, soluble in water, can be used for the fields such as food and health care.
Embodiment
Below in conjunction with embodiment, the present invention is described in further details.
Embodiment 1
(a) to take the fresh jellyfish of 500kg be raw material in the processing of raw material, and clear water soaks desalination to salt and is divided into 3%, cleans up rear employing colloidal mill and carry out milled processed, and gained jellyfish slurry particle fineness is controlled at 3.5um;
(b) the first step enzymolysis determination step (a) gained jellyfish slurry moisture and protein content, according to measurement result, adding water, to adjust the mass concentration of protein be 2%; It is 3.26 that acid adding is adjusted the pH value, and controlling temperature is 37 ℃, adds stomach en-, and pepsic consumption is the stomach en-that every gram protein adds 3000u, and insulation enzymolysis 4h, obtain jellyfish pepsin hydrolysis liquid;
(c) the pH value of second step enzymolysis jellyfish pepsin hydrolysis liquid that step (b) is obtained is adjusted to 5.02 with alkaline solution, temperature rises to 50 ℃, add papoid, the papoid consumption is the papoid that every gram protein adds 4000u, enzymolysis 1h, obtain the two enzyme enzymolysis solutions of jellyfish;
(d) enzyme-deactivating is heated to 95 ℃ with the two enzyme enzymolysis solutions of jellyfish that step (c) obtains, and keeps 15 minutes, carries out enzyme-deactivating, the two enzyme enzymolysis solutions of the jellyfish of the enzyme that must go out;
(e) impurity elimination, desalination are removed impurity with the two enzyme enzymolysis solutions of the enzyme jellyfish of going out that step (d) obtains through tubular membrane, wherein tubular membrane work aperture is 0.15 μ m, working temperature is controlled at 35 ℃, advances film pressure and is controlled at 2.5bar, and the membrane pressure-controlling is at 0.9bar.Then adopt the nanofiltration membrane desalination of 250 trapped molecular weights, concentrate in the time of desalination, resulting concentrated solution can further adopt vacuum concentration, obtains jellyfish Antihypertensive Peptides concentrated solution, and the soluble solid content of this jellyfish Antihypertensive Peptides concentrated solution is 20%;
(f) the dry jellyfish Antihypertensive Peptides concentrated solution that step (e) is obtained adopts cryodesiccated mode to carry out drying, cryodesiccated working conditions is: vacuum degree control is at 100Pa, and temperature of heating plate: 20 ℃ 250min/40 ℃ 400min/30 ℃ until the freeze-dried material constant weight; Condenser temperature is controlled at-45 ℃.Obtain jellyfish step-down Gly-His-Lys 3.5kg, measure its IC according to external ACE inhibiting rate
50For 0.4mg/mL.
Embodiment 2
(a) to take the alum of getting 500kg jellyfish processed be raw material in the processing of raw material, and clear water immersion, rehydration, desalination are to salinity＜5%, and flowing water cleans up rear employing colloidal mill and carries out milled processed, and gained jellyfish slurry particle fineness is controlled at 2um;
(b) the first step enzymolysis determination step (a) gained jellyfish slurry moisture and protein content, according to measurement result, adding water, to adjust the mass concentration of protein be 3%; It is 2 that acid adding is adjusted the pH value, and controlling temperature is 35 ℃, adds stomach en-, and pepsic consumption is the stomach en-that every gram protein adds 4000u, and insulation enzymolysis 5h, obtain jellyfish pepsin hydrolysis liquid;
(c) pH of second step enzymolysis jellyfish pepsin hydrolysis liquid that step (b) is obtained is adjusted to 6 with alkaline solution, controlling temperature is 55 ℃, adds papoid, and the papoid consumption is the papoid that every gram protein adds 3500u, enzymolysis 2h, obtain the two enzyme enzymolysis solutions of jellyfish;
(d) enzyme-deactivating is heated to 100 ℃ with the two enzyme enzymolysis solutions of jellyfish that step (c) obtains, and keeps 10 minutes, carries out enzyme-deactivating, the two enzyme enzymolysis solutions of the jellyfish of the enzyme that must go out;
(e) impurity elimination, desalination are removed impurity with the two enzyme enzymolysis solutions of the enzyme jellyfish of going out that step (d) obtains through tubular membrane, wherein the work aperture of tubular membrane is 0.2 μ m, working temperature is controlled at 30 ℃, advances film pressure and is controlled at 3bar, and the membrane pressure-controlling is at 1.0bar.Then adopt the nanofiltration membrane desalination of 300 trapped molecular weights, concentrate in the time of desalination, the concentrated solution that obtains further adopts the vacuum concentration mode to concentrate, and obtains jellyfish Antihypertensive Peptides concentrated solution, and the soluble solid content of this jellyfish Antihypertensive Peptides concentrated solution is 15%;
(f) the dry jellyfish Antihypertensive Peptides concentrated solution that step (e) is obtained carries out spraying drying, and during spraying drying, its inlet temperature is controlled at 190 ℃, and air outlet temperature is controlled at 75 ℃, spray pressure is 0.4MPa, obtain jellyfish step-down Gly-His-Lys 4.0kg, measure its IC according to external ACE inhibiting rate
50For 0.5mg/mL.
Embodiment 3
(a) to take 300kg alum jellyfish processed be raw material in the processing of raw material, and clear water immersion, rehydration, desalination, to salinity＜4%, adopt colloidal mill to carry out milled processed after flowing water is cleaned, and gained jellyfish slurry particle fineness is controlled at 5um;
(b) moisture and the protein content of the first step enzymolysis determination step (a) gained jellyfish slurry, the mass concentration that adds water adjustment protein is 1%; It is 3 that acid adding is adjusted the pH value, and controlling temperature is 40 ℃, adds stomach en-, and pepsic consumption is the stomach en-that every gram protein adds 2000u, and insulation enzymolysis 6h, obtain jellyfish pepsin hydrolysis liquid;
(c) the second step enzymolysis is adjusted pH value to 5.5 with the jellyfish pepsin hydrolysis liquid that step (b) obtains with alkaline solution, controlling temperature is 52 ℃, add papoid, the papoid consumption is the papoid that every gram protein adds 2000u, enzymolysis 3h, obtain the two enzyme enzymolysis solutions of jellyfish;
(d) enzyme-deactivating is heated to 90 ℃ with the two enzyme enzymolysis solutions of jellyfish that step (c) obtains, and keeps 20 minutes, carries out enzyme-deactivating, the two enzyme enzymolysis solutions of the enzyme jellyfish of must going out;
(e) impurity elimination, desalination are removed impurity with the two enzyme enzymolysis solutions of the enzyme jellyfish of going out that step (d) obtains through tubular membrane, the work aperture of its tubular membrane is 0.1 μ m, working temperature is controlled at 40 ℃, advances film pressure and is controlled at 2.8bar, and the membrane pressure-controlling is at 0.8bar.Then adopt the nanofiltration membrane desalination of 150 trapped molecular weights, concentrate in the time of desalination, the concentrated solution that obtains adopts the vacuum concentration mode further concentrated, obtains jellyfish Antihypertensive Peptides concentrated solution, and the soluble solid content of this jellyfish Antihypertensive Peptides concentrated solution is 18%;
(f) the dry jellyfish Antihypertensive Peptides concentrated solution that step (e) is obtained carries out lyophilize, and cryodesiccated working conditions is: vacuum degree control is at 133Pa, and temperature of heating plate: 20 ℃ 250min/40 ℃ 400min/30 ℃ until the freeze-dried material constant weight; Condenser temperature is controlled at-50 ℃, obtains jellyfish step-down Gly-His-Lys 2.5kg, measures its IC according to external ACE inhibiting rate
50For 0.4mg/mL.
Adopt the SHR rat model to estimate prepared jellyfish step-down Gly-His-Lys, experimental result such as table 1.
Each administration group of table 1 on the impact of SHR caudal artery systolic pressure (
MmHg)
Annotate: compare * P＜0.05, * * P＜0.01 with the blank group
Compare with the blank group, jellyfish Antihypertensive Peptides group rat oral gavage is all obviously reductions (P＜0.05) of blood pressure level after 4 weeks, and the administration group has no significant effect rat body weight.The jellyfish Antihypertensive Peptides can obviously reduce the blood pressure of original hypertensive rat.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101002208A CN102191306B (en) | 2011-04-09 | 2011-04-09 | Enzymatic preparation method for antihypertensive peptides from jellyfish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101002208A CN102191306B (en) | 2011-04-09 | 2011-04-09 | Enzymatic preparation method for antihypertensive peptides from jellyfish |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102191306A CN102191306A (en) | 2011-09-21 |
| CN102191306B true CN102191306B (en) | 2013-11-13 |
Family
ID=44600173
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101002208A Active CN102191306B (en) | 2011-04-09 | 2011-04-09 | Enzymatic preparation method for antihypertensive peptides from jellyfish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102191306B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102409072B (en) * | 2011-11-29 | 2014-07-23 | 山东好当家海洋发展股份有限公司 | Method for preparing composite blood pressure lowering peptide by using marine organism |
| CN103340448A (en) * | 2013-06-09 | 2013-10-09 | 中国海洋大学 | Jellyfish crystal jelly and processing method thereof |
| CN104524541B (en) * | 2014-11-27 | 2018-05-18 | 山东好当家海洋发展股份有限公司 | A kind of jellyfish albumen effervescent tablet and preparation method thereof |
| CN104498571B (en) * | 2014-12-05 | 2017-10-20 | 中南林业科技大学 | A kind of Extracted From Oil-tea-cake protein blood pressure-reducing peptide batch (-type) preparation method |
| CN104774255B (en) * | 2015-04-14 | 2018-01-02 | 山东省科学院生物研究所 | The biologically active peptide in jellyfish source and preparation method thereof |
| CN105037486A (en) * | 2015-08-07 | 2015-11-11 | 国家海洋局第三海洋研究所 | Method for preparing jellyfish active glycoprotein through multi-stage film separation technology |
| CN105494839A (en) * | 2015-12-26 | 2016-04-20 | 山东好当家海洋发展股份有限公司 | Rhopilema esculentum polypeptide composite tablet candy and preparation method thereof |
| CN106591409A (en) * | 2016-12-30 | 2017-04-26 | 浙江海洋大学 | Squid ink oligopeptide with lipid metabolism regulation function |
| CN109548950A (en) * | 2018-12-20 | 2019-04-02 | 水母娘娘海洋生物科技有限公司 | The polypeptide and its preparation process in a kind of aurelia source and application |
| CN113812583B (en) * | 2021-08-09 | 2023-12-22 | 庄臣酿酒（福建）有限公司 | Preparation method of jellyfish extract |
| CN114767831A (en) * | 2022-04-21 | 2022-07-22 | 中国科学院海洋研究所 | Application of jellyfish polypeptide in preparation of weight-reducing medicine |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101862375A (en) * | 2010-05-26 | 2010-10-20 | 山东好当家海洋发展股份有限公司 | A kind of compound jellyfish antihypertensive granule and preparation method thereof |
-
2011
- 2011-04-09 CN CN2011101002208A patent/CN102191306B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101862375A (en) * | 2010-05-26 | 2010-10-20 | 山东好当家海洋发展股份有限公司 | A kind of compound jellyfish antihypertensive granule and preparation method thereof |
Non-Patent Citations (10)
| Title |
|---|
| Angiotensin converting enzyme (ACE) inhibitory, antihypertensive and antihyperlipidaemic activities of protein hydrolysates from Rhopilema esculentum;Xin Liu 等;《Food Chemistry》;20121015;第134卷(第4期);2134-2140 * |
| XinLiu等.Angiotensinconvertingenzyme(ACE)inhibitory antihypertensive and antihyperlipidaemic activities of protein hydrolysates from Rhopilema esculentum.《Food Chemistry》.2012 |
| 刘景侠 等.海蜇降压肽制备工艺及体内降压效果的研究.《食品科学》.2009,第30卷(第11期), |
| 刘景侠.海蜇降压肽提取工艺研究与效果测定.《中国优秀硕士学位论文全文数据库 工程科技I辑》.2009,(第11期), |
| 安桂香.海蜇活性肽的制备及降血压效果的研究.《中国优秀硕士学位论文全文数据库 工程科技I辑》.2007,(第3期), |
| 张绵松 等.海蜇血管紧张素转化酶抑制肽的超滤分离.《食品与药品》.2010,第12卷(第1期), |
| 海蜇活性肽的制备及降血压效果的研究;安桂香;《中国优秀硕士学位论文全文数据库 工程科技I辑》;20070315(第3期);25，27，34-35 * |
| 海蜇血管紧张素转化酶抑制肽的超滤分离;张绵松 等;《食品与药品》;20100110;第12卷(第1期);20-23 * |
| 海蜇降压肽制备工艺及体内降压效果的研究;刘景侠 等;《食品科学》;20090601;第30卷(第11期);227-231 * |
| 海蜇降压肽提取工艺研究与效果测定;刘景侠;《中国优秀硕士学位论文全文数据库 工程科技I辑》;20091115(第11期);55-56 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102191306A (en) | 2011-09-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102191306B (en) | Enzymatic preparation method for antihypertensive peptides from jellyfish | |
| CN105361153B (en) | Processing method of sea cucumber glycopeptide chelated calcium | |
| CN103205480B (en) | Method for producing high-quality collagen oligopeptide by using fish skin or fishbone | |
| CN102115774B (en) | Method for preparing plant polypeptide by enzyme process | |
| CN102204620B (en) | Method for preparing bitterless protein peptides | |
| CN102599546B (en) | A method of desalting and drying salted egg white to prepare desalted protein powder | |
| JP6046779B2 (en) | Rice protein composition and method for producing the same | |
| CN102533914A (en) | High-purity fishy smell and foreign odor-free fish collagen protein peptide and preparation method thereof | |
| CN110178964A (en) | The preparation process of sturgeon protein peptides | |
| CN107950669A (en) | A kind of starch of high-protein bean containing peptide and its preparation process | |
| CN101845080A (en) | Angiotensin converting enzyme inhibitory peptide and preparation method thereof | |
| CN107446978A (en) | A kind of stem of noble dendrobium small-molecular peptides and preparation method thereof | |
| CN102488713A (en) | Method for preparing sheep placenta extract and sheep placenta hydrolyzed collagen concentrated solution | |
| CN102409072A (en) | Method for preparing composite antihypertensive peptide by using marine organisms | |
| CN104366645A (en) | Preparation method of mumefural | |
| CN103045707A (en) | Method for preparing rice protein active polypeptide through continuous enzymolysis and secondary membrance filtering and purification | |
| CN1896267B (en) | Preparation of depressor peptide by silkworm chrysalis | |
| CN103636914A (en) | Oyster peptide extraction method | |
| CN105272887B (en) | A kind of method for extracting taurine and polysaccharide in the internal organ from abalone simultaneously | |
| CN104509679B (en) | A kind of method that utilization defatted soybean meal produces soy glycoproteins | |
| CN107753927A (en) | Chinese medicine composition of the peptide containing the membrane of a chicken's gizzard with promoting digestion and removing indigestion effect and its production and use | |
| CN107674905A (en) | Spirulina bioactive peptide, composition and preparation method | |
| CN103876163A (en) | Rana japonica meat composite protein powder and preparation method thereof | |
| CN107828840A (en) | Silkworm chrysalis active peptide, composition and preparation method | |
| CN100469894C (en) | Method for preparing laver antihypertensive peptide by enzyme-membrane coupling technology and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |