CN107372370A - Hatching solution for hatching Aedes albopictus eggs and hatching method for Aedes albopictus eggs - Google Patents

Abstract

The invention relates to a hatching liquid for hatching Aedes albopictus eggs and a method for hatching the eggs of Aedes albopictus. The hatching solution is an aqueous solution prepared from yeast peptone and water, and the concentration of yeast peptone in the aqueous solution is 0.7-1.5 g/L. The hatching method comprises the following steps: fermenting the hatching liquid used for hatching Aedes albopictus eggs; weighing the eggs of Aedes albopictus and adding them into an incubation tube, and adding the fermented eggs into the incubation tube Seal the cap of the incubation tube, shake well, let it stand for incubation, then open the cap of the incubation tube, and transfer the hatched larvae to the rearing tray. Using this hatching solution to incubate Aedes albopictus eggs under certain conditions can greatly speed up the hatching rate of Aedes albopictus eggs, the hatching rate of active mosquito eggs within 1 hour is the highest, and the larval growth synchronization is good. Moreover, the hatching method of the present invention has high larvae survival rate, simple operation and low hatching cost.

Description

Hatching solution for hatching Aedes albopictus eggs and hatching method for Aedes albopictus eggs

technical field

The invention relates to the technical field of artificial breeding of Aedes albopictus, in particular to a hatching solution for hatching eggs of Aedes albopictus and a method for hatching eggs of Aedes albopictus.

Background technique

As an emerging technology in pest control, sterile male insect release technology is used to reduce or even eliminate the insects by cultivating sterile male insects and releasing them to the breeding grounds of pests. After mating with wild female insects, they cannot reproduce offspring. Compared with traditional insecticide deworming strategies, this technology is environmentally friendly and has no impact on other insects and the environment. Mosquitoes serve as intermediate hosts for dengue, Zika, malaria, yellow fever, filariasis, Japanese encephalitis, and other pathogens. In the large-scale Aedes albopictus male mosquito release program, the incubation of mosquito eggs is an important link, so it is necessary to develop a simple, efficient and stable method for mosquito egg incubation.

The hatching rate of Aedes albopictus eggs in anaerobic environment is much faster than that in aerobic environment. The traditional hatching method of Aedes albopictus eggs is to heat the water to saturation under normal pressure through thermal deoxygenation. The temperature reduces the solubility of oxygen in water, and oxygen escapes from water to form an oxygen-free environment. Then quickly transfer the anaerobic water to a closed container, fill it with a cover, cool it to 25°C-30°C, weigh the required mosquito eggs, open the cover and pour it in, shake it well, seal the cover again, and place it at 25°C-30°C. Incubate at a temperature of 30° C. for 1 hour, finally open the container, and transfer the hatched larvae to rearing trays. This traditional technology has a general effect on promoting the hatching of Aedes albopictus. Only less than 80% of the active mosquito eggs can be hatched in one hour, and the remaining mosquito eggs are gradually hatched after being transferred to the rearing tray, and the larval growth synchronization is poor. The oxygen content in the airtight container is low, and the larvae that hatch first are easy to die. The incubation process requires heating, high energy consumption, cumbersome operation, and strict requirements on the airtightness of the container. It is not conducive to the standardized operation and cost control of mass production.

Contents of the invention

Based on this, the present invention provides a hatching solution for hatching Aedes albopictus eggs, hatching Aedes albopictus eggs with the hatching solution can greatly accelerate the hatching rate of Aedes albopictus eggs, active mosquito eggs The hatching rate is high within 1 hour, which makes the larval growth synchronization good.

The specific technical scheme is as follows:

A hatching solution for hatching eggs of Aedes albopictus, the hatching solution is an aqueous solution prepared from yeast peptone and water, and the concentration of yeast peptone in the aqueous solution is 0.7-1.5 g/L.

Preferably, the concentration of yeast peptone in the aqueous solution is 0.8-1.0 g/L.

Preferably, the concentration of yeast peptone in the aqueous solution is 0.9 g/L.

Preferably, the yeast peptone is angel yeast peptone.

The invention also provides a method for hatching the eggs of Aedes albopictus. The method can greatly accelerate the hatching rate of the mosquito eggs of Aedes albopictus, and the hatching rate of the active mosquito eggs within one hour is high, so that the synchronization of larval growth is good.

The specific technical scheme is as follows:

A method for hatching the eggs of Aedes albopictus, comprising the following steps:

Fermenting the hatching solution used to hatch the eggs of Aedes albopictus to obtain the fermented hatching solution; the hatching solution is an aqueous solution prepared from yeast peptone and water, and the concentration of yeast peptone in the aqueous solution is 0.7-1.5 g/L;

Weigh the eggs of Aedes albopictus and add them to the hatching tube, add the fermented hatching solution into the hatching tube, seal the cap of the hatching tube, shake well, leave it to hatch, and open the hatch of the hatching tube after the hatching is over. Cap the tube and transfer the hatched larvae to a rearer dish.

Preferably, the hatching solution is an aqueous solution prepared from yeast peptone and water, and the concentration of yeast peptone in the aqueous solution is 0.8-1.0 g/L.

Preferably, the hatching solution is an aqueous solution prepared from yeast peptone and water, and the concentration of yeast peptone in the aqueous solution is 0.9 g/L.

Preferably, the yeast peptone is angel yeast peptone.

Preferably, the fermentation temperature is 25°C-30°C, and the fermentation time is 20-28 hours.

Preferably, the added amount of the fermented hatching liquid is 50-80% of the volume of the hatching tube.

Preferably, the added amount of the fermented hatching liquid is 60-70% of the volume of the hatching tube.

Preferably, the ratio of the Aedes albopictus eggs to the fermented hatching liquid is 1 g: 150-350 mL.

Preferably, the temperature of the static incubation is 25°C-30°C, and the time of the static incubation is 0.8-1.2 hours.

The hatching solution for hatching Aedes albopictus eggs of the present invention and the hatching method of Aedes albopictus eggs have the following advantages and beneficial effects:

The inventors of the present invention unexpectedly discovered in their long-term experience accumulation and experimental process that using yeast peptone to prepare an aqueous solution with a specific concentration as the hatching solution for hatching Aedes albopictus eggs can obtain a stable and efficient Aedes albopictus The hatching method of mosquito eggs can greatly speed up the hatching rate of Aedes albopictus eggs, and the hatching effect is much better than the existing hatching method of thermal deoxygenation. Yeast peptone is used to configure the hatching solution of Aedes albopictus mosquito eggs. When using the hatching solution, it is first fermented. During the fermentation, the yeast colonies that multiply and increase in large numbers consume the oxygen in the solution through respiration, creating a kind that can promote albopictus. Oxygen-free environment for rapid hatching of Aedes mosquito eggs. Using this hatching solution to incubate Aedes albopictus eggs under certain conditions can greatly speed up the hatching rate of Aedes albopictus eggs, the hatching rate of active mosquito eggs is high within 1 hour, and the larval growth synchronization is good.

The hatching method of the present invention has no strict requirements on the airtightness of the hatching tube, and leaving a certain amount of oxygen in the hatching tube will be more conducive to the hatching effect. And death, the larval survival rate is high, creating a prerequisite for further large-scale standardized breeding of Aedes albopictus.

In the hatching method of the present invention, only yeast peptone and water are needed for the configuration of the hatching solution, and the configuration process and the hatching operation process are very simple, without energy consumption, and the hatching cost is greatly reduced.

detailed description

The hatching solution for hatching Aedes albopictus eggs and the method for hatching Aedes albopictus eggs of the present invention will be further described in detail below in conjunction with specific examples.

The yeast peptone used in the following examples is Angel Yeast Peptone FP101, which is a stable, safe and nutritionally comprehensive product obtained from purely cultured high-protein baker’s yeast through separation and enrichment of yeast protein treatment and compound enzyme action. Peptone. Its content is as follows, total nitrogen: ≥10.0%, a-amino nitrogen: ≥2.5%, moisture: ≤6.0%, NaCl: ≤2.0%, ash content: ≤15.0%, pH (2% solution): 5.3-7.2.

Example 1

The present embodiment provides a method for hatching eggs of Aedes albopictus, comprising the following steps:

Take yeast peptone and add water to prepare an aqueous solution with a concentration of 0.9g/L as the hatching solution;

Place the hatching liquid at a temperature of 25°C to 30°C to ferment for 24 hours to obtain a fermented hatching liquid;

Weigh 0.14g of Aedes albopictus eggs into a 50mL incubation tube, and add 33.3mL of fermented hatching liquid into the hatching tube (the fermented hatching liquid accounts for 2/3 of the volume of the hatching tube); seal the tube of the hatching tube Cover, shake well, place horizontally, and incubate at a temperature of 25°C to 30°C for 1 hour, then open the cap of the incubation tube, and transfer the hatched larvae to a rearing tray.

Example 2

The present embodiment provides a method for hatching eggs of Aedes albopictus, comprising the following steps:

Take yeast peptone and add water to prepare an aqueous solution with a concentration of 0.9g/L as the hatching solution;

Place the hatching liquid at a temperature of 25°C to 30°C to ferment for 24 hours to obtain a fermented hatching liquid;

Weigh 0.14g Aedes albopictus ovum and add in the 50mL incubation tube, add 25mL fermented hatching liquid in the hatching tube (the hatching liquid after fermentation accounts for 50% of the hatching tube volume); Seal the tube lid of the hatching tube, Shake well, and incubate at a temperature of 25° C. to 30° C. for 1 hour, then open the cap of the incubation tube, and transfer the hatched larvae to a rearing tray.

Example 3

The present embodiment provides a method for hatching eggs of Aedes albopictus, comprising the following steps:

Take yeast peptone and add water to prepare an aqueous solution with a concentration of 0.9g/L as the hatching solution;

Place the hatching liquid at a temperature of 25°C to 30°C to ferment for 24 hours to obtain a fermented hatching liquid;

Weigh 0.14g Aedes albopictus ovum and add in the 50mL incubation tube, add 40mL fermented hatching liquid in the hatching tube (the hatching liquid after fermentation accounts for 80% of the hatching tube volume); Seal the tube lid of the hatching tube, Shake well, and incubate at a temperature of 25° C. to 30° C. for 1 hour, then open the cap of the incubation tube, and transfer the hatched larvae to a rearing tray.

Example 4

The present embodiment provides a method for hatching eggs of Aedes albopictus, comprising the following steps:

Take yeast peptone and add water to prepare an aqueous solution with a concentration of 0.8g/L as the hatching solution;

Place the hatching liquid at a temperature of 25°C to 30°C to ferment for 24 hours to obtain a fermented hatching liquid;

Weigh 0.14g of Aedes albopictus eggs into a 50mL incubation tube, and add 33.3mL of fermented hatching liquid into the hatching tube (the fermented hatching liquid accounts for 2/3 of the volume of the hatching tube); seal the tube of the hatching tube Cover, shake well, and incubate at a temperature of 25° C. to 30° C. for 1 hour, then open the cap of the incubation tube, and transfer the hatched larvae to a rearing tray.

Example 5

The present embodiment provides a method for hatching eggs of Aedes albopictus, comprising the following steps:

Take yeast peptone and add water to prepare an aqueous solution with a concentration of 1.4g/L as the hatching solution;

Place the hatching liquid at a temperature of 25°C to 30°C to ferment for 24 hours to obtain a fermented hatching liquid;

Weigh 0.14g of Aedes albopictus eggs into a 50mL incubation tube, and add 33.3mL of fermented hatching liquid into the hatching tube (the fermented hatching liquid accounts for 2/3 of the volume of the hatching tube); seal the tube of the hatching tube Cover, shake well, and incubate at a temperature of 25° C. to 30° C. for 1 hour, then open the cap of the incubation tube, and transfer the hatched larvae to a rearing tray.

Comparative example 1

The present embodiment provides a method for hatching eggs of Aedes albopictus, comprising the following steps:

Take yeast peptone and add water to prepare an aqueous solution with a concentration of 2.0g/L as the hatching solution;

Place the hatching liquid at a temperature of 25°C to 30°C to ferment for 24 hours to obtain a fermented hatching liquid;

Weigh 0.14g of Aedes albopictus eggs into a 50mL incubation tube, and add 33.3mL of fermented hatching liquid into the hatching tube (the fermented hatching liquid accounts for 2/3 of the volume of the hatching tube); seal the tube of the hatching tube Cover, shake well, and incubate at a temperature of 25° C. to 30° C. for 1 hour, then open the cap of the incubation tube, and transfer the hatched larvae to a rearing tray.

Comparative example 2

The present embodiment provides a method for hatching eggs of Aedes albopictus, comprising the following steps:

Take yeast peptone and add water to prepare an aqueous solution with a concentration of 0.1g/L as the hatching solution;

Place the hatching liquid at a temperature of 25°C to 30°C to ferment for 24 hours to obtain a fermented hatching liquid;

Weigh 0.14g of Aedes albopictus eggs into a 50mL incubation tube, and add 33.3mL of fermented hatching liquid into the hatching tube (the fermented hatching liquid accounts for 2/3 of the volume of the hatching tube); seal the tube of the hatching tube Cover, shake well, and incubate at a temperature of 25° C. to 30° C. for 1 hour, then open the cap of the incubation tube, and transfer the hatched larvae to a rearing tray.

Comparative example 3

The present embodiment provides a method for hatching eggs of Aedes albopictus, comprising the following steps:

Take yeast peptone and add water to prepare an aqueous solution with a concentration of 0.9g/L as the hatching solution;

Place the hatching liquid at a temperature of 25°C to 30°C to ferment for 24 hours to obtain a fermented hatching liquid;

Weigh 0.14g Aedes albopictus ovum and add in the 50mL incubation tube, add 15mL fermented hatching liquid (the fermented hatching liquid accounts for 30% of the hatching tube volume) in the hatching tube; Seal the tube lid of the hatching tube, Shake well, and incubate at a temperature of 25° C. to 30° C. for 1 hour, then open the cap of the incubation tube, and transfer the hatched larvae to a rearing tray.

Comparative example 4

The present embodiment provides a method for hatching eggs of Aedes albopictus, comprising the following steps:

Take yeast peptone and add water to prepare an aqueous solution with a concentration of 0.9g/L as the hatching solution;

Place the hatching liquid at a temperature of 25°C to 30°C to ferment for 24 hours to obtain a fermented hatching liquid;

Weigh 0.14g of Aedes albopictus eggs into a 50mL incubation tube, and add 47.5mL of fermented hatching solution into the hatching tube (the fermented hatching solution accounts for 95% of the volume of the hatching tube); seal the cap of the hatching tube , shake well, and incubate at a temperature of 25° C. to 30° C. for 1 hour, then open the cap of the incubation tube, and transfer the hatched larvae to a rearing tray.

The hatching rate of the active mosquito ovum of embodiment 1-5 and comparative example 1-4 and the statistic result of larval survival rate are shown in table 1 below, by the result in the table as seen: the concentration of yeast peptone in the hatching liquid and the hatching liquid in the hatching tube The amount of addition has a decisive effect on the hatchability and larval survival rate. Wherein, the effect of embodiment 1-5 is far better than comparative example 1-4, and the effect of embodiment 1-3 is the best, and the hatching rate of active mosquito egg and larva survival rate are all very high; The concentration of yeast peptone is low, and the hatching rate of active mosquito eggs is lower than that of Examples 1-3, but it does not affect its larval survival rate; in Example 5, the hatching rate of active mosquito eggs is high due to the high concentration of yeast peptone in the hatching solution , but the hatched larva survival rate is lower than that of Examples 1-3. Comparative example 1 is compared with embodiment 1, and the concentration of yeast peptone in its hatching liquid is too high, causes the larva survival rate of hatching to be extremely low; Comparative example 2 is compared with embodiment 1, and the concentration of yeast peptone in its hatching liquid is too high. Low, resulting in very low hatching rate of active mosquito eggs; comparative example 3 compared with embodiment 1, the addition of hatching liquid is too little, resulting in low hatching rate of active mosquito eggs, and the larva survival rate of hatched is low; comparative example 4 Compared with Example 1, the amount of hatching solution added is too much, resulting in a low survival rate of larvae.

Table 1

Hatch rate of live mosquito eggs Larval survival rate Example 1 98.34% 99.32% Example 2 95.28% 99.15% Example 3 97.16% 99.43% Example 4 85.67% 99.28% Example 5 98.11% 74.92% Comparative example 1 98.39% 3.51% Comparative example 2 12.53% 99.19% Comparative example 3 35.56% 83.79% Comparative example 4 96.45% 37.07%

The above-mentioned embodiments only express several implementation modes of the present invention, and the descriptions thereof are relatively specific and detailed, but should not be construed as limiting the patent scope of the invention. It should be noted that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent for the present invention should be based on the appended claims.

Claims (10)

1. a kind of be used to hatch the artemia hatching solution of aedes albopictus mosquito ovum, it is characterised in that the artemia hatching solution be by Yeast protein peptone and Water prepares the obtained aqueous solution, and the concentration of Yeast protein peptone is 0.7-1.5g/L in the aqueous solution.

2. the artemia hatching solution according to claim 1 for being used to hatch aedes albopictus mosquito ovum, it is characterised in that in the aqueous solution The concentration of Yeast protein peptone is 0.8-1.0g/L.

3. the artemia hatching solution according to claim 2 for being used to hatch aedes albopictus mosquito ovum, it is characterised in that in the aqueous solution The concentration of Yeast protein peptone is 0.9g/L.

4. the artemia hatching solution for being used to hatch aedes albopictus mosquito ovum according to claim any one of 1-3, it is characterised in that described Yeast protein peptone is Angel Yeast peptone.

5. a kind of hatching method of aedes albopictus mosquito ovum, it is characterised in that comprise the following steps：

The artemia hatching solution for being used to hatch aedes albopictus mosquito ovum described in claim any one of 1-4 is fermented, after obtaining fermentation Artemia hatching solution；

Weigh aedes albopictus mosquito ovum to add in hatching pipe, the artemia hatching solution added in the hatching pipe after the fermentation, seal up and incubate Change the lid of pipe, shake up, stand hatching, hatching opens the lid of hatching pipe, the larva hatched is transferred into raising after terminating In disk.

6. the hatching method of aedes albopictus mosquito ovum according to claim 5, it is characterised in that the temperature of the fermentation is 25 DEG C~30 DEG C, the time of the fermentation is 20-28 hours.

7. the hatching method of aedes albopictus mosquito ovum according to claim 5, it is characterised in that the artemia hatching solution after the fermentation Addition for it is described hatching pipe volume 50-80%.

8. the hatching method of aedes albopictus mosquito ovum according to claim 7, it is characterised in that the artemia hatching solution after the fermentation Addition for it is described hatching pipe volume 60-70%.

9. the hatching method of the aedes albopictus mosquito ovum according to claim any one of 5-8, it is characterised in that the lineae ablicantes she The proportioning of mosquito mosquito ovum and the artemia hatching solution after the fermentation is 1g：150-350mL.

10. the hatching method of the aedes albopictus mosquito ovum according to claim any one of 5-8, it is characterised in that the standing The temperature of hatching is 25 DEG C~30 DEG C, and the time for standing hatching is 0.8-1.2 hours.