CN107320522A - Chinese medicine composition and preparation method are grown for bird immunity - Google Patents

Abstract

A traditional Chinese medicine composition for poultry immune growth and a preparation method thereof, which is composed of echinacea purpurea, astragalus, ginkgo pulp, honeysuckle, and licorice. It can overcome the disadvantages of reducing the production performance of the body, causing toxic side effects and drug residues, and endangering human health in the presence of chemical drug immune enhancers. The traditional Chinese medicine composition used for poultry immune growth can not only improve the disease resistance and resistance of the body, but also can improve the weight gain and feed conversion rate of the poultry, and produce good economic benefits.

Description

Traditional Chinese medicine composition for poultry immune growth and preparation method

Technical field:

The invention relates to a traditional Chinese medicine composition for poultry immune growth, which is used in poultry feeding, and can increase spleen and bursa index, synthesis of NDV-IV and IBDV antibodies, chicken serum IL-6 and IFN-γ after drinking. , IL-4 and IL-12 content, increase weight gain and feed conversion rate.

technical background:

In recent years, there is a common problem of poor vaccination effect in chicken production. This is due to the long-term abuse of a large number of drugs and unreasonable vaccination, which leads to the decline of immune function of chickens, especially the immune cells such as lymphocytes. Depressed or depressed state. In addition, new epidemic diseases continue to appear in recent years, and the pathogenic mutation and virulence of old epidemic diseases are enhanced, which makes chicken infectious diseases continue to break out. To solve this problem, it is necessary to take effective measures to activate the immune system and improve the activity of immune cells, so that the chicken body can obtain a higher level of immune response after vaccination and enhance the body's disease resistance. The currently used chemical drug immune enhancers have played a positive role in the healthy development of the chicken industry, but the chemical drug immune enhancers all have disadvantages such as reducing the production performance of the body to varying degrees, causing toxic side effects and drug residues, and endangering human health. . Traditional Chinese medicine has the advantages of low toxicity and less residue. Therefore, seeking Chinese medicine as an immune enhancer can not only improve the body's disease resistance and resistance, but also improve the weight gain and feed conversion rate of poultry, resulting in good economic benefits.

Invention content:

The purpose of the present invention is to overcome the above-mentioned defects, to provide a kind of immunogrowth for poultry that can not only improve the disease resistance and resistance of poultry, but also can improve the weight gain and feed conversion rate of poultry, with low cost and little toxic and side effects. Traditional Chinese medicine composition and preparation method.

The present invention is achieved through the following technical approaches:

The traditional Chinese medicine composition for poultry immune growth of the present invention consists of 30-50 parts by mass of echinacea, 15-25 parts of astragalus, 15-25 parts of ginkgo pulp, 8-12 parts of honeysuckle and 8-12 parts of licorice. Its preparation method is:

According to the number of parts by mass, take 30-50 pieces of echinacea, 15-25 pieces of astragalus, 15-25 pieces of ginkgo pulp, 8-12 pieces of honeysuckle, and 8-12 pieces of licorice. Soak in distilled water for 12-18 hours, then boil for 2 hours, filter, and collect the filtrate; add 5 times the amount of distilled water to the filter residue, boil for 2 hours, filter, collect the filtrate, combine the two filtrates, and concentrate to a drug solution containing 1g of drug per mL.

Usage and Dosage:

The traditional Chinese medicine composition is added in the free drinking water of poultry according to the concentration of 4% of the water volume.

The Chinese herbal medicine raw materials used in the present invention can be purchased from traditional Chinese medicine stores and pharmaceutical companies, and its specifications meet the national medical standards.

The above traditional Chinese medicine composition of the present invention can be prepared into a commonly used oral preparation by adding appropriate additives (commonly used flavoring agents, bacteriostats, antioxidants, coloring agents, etc.), dissolved and mixed, filtered and clarified, such as oral liquid etc.

Benefits of the present invention:

1. Produce high-quality, low drug residue, green and pollution-free poultry products

In recent years, the research of chemical drug immune enhancers has made great progress, which has played a positive role in the healthy development of the chicken industry, but chemical drug immune enhancers all exist to varying degrees to reduce the production performance of the body and cause toxic and side effects and drug residues, endangering human health and other disadvantages. Traditional Chinese medicine immune enhancers have attracted much attention because of their naturalness, wide range of effects, treating both symptoms and root causes, safety and low toxicity, not easy to produce drug resistance, no pollution, and no residue. The traditional Chinese medicine composition of the invention can not only improve the disease resistance and resistance of the body, but also can increase the weight gain and feed conversion rate of poultry, and produce good economic benefits.

2. Improve the disease resistance and resistance of poultry

The prominent feature of the traditional Chinese medicine composition of the invention is that the composition contains echinacea purpurea, astragalus, ginkgo pulp, honeysuckle and licorice. The traditional Chinese medicine composition composed of five kinds of traditional Chinese medicine raw materials can produce synergistic effect to stimulate the immune response of poultry body, enhance the immune function and improve the curative effect. The poultry immune growth traditional Chinese medicine composition has the functions of invigorating qi and promoting yang, invigorating spleen and qi, invigorating deficiency and strengthening the body, astringing lung qi, relieving asthma and cough, alleviating medicinal properties, clearing heat and detoxifying, harmonizing various medicines, anti-inflammatory and antibacterial effects. After poultry drinking, it can increase spleen, bursa index, NDV-Ⅳ, IBDV antibody synthesis, chicken serum IL-6, IFN-γ, IL-4 and IL-12 content, increase weight gain and feed conversion rate.

The main ingredient of Echinacea is cichoric acid. Chichoric acid is a derivative of caffeic acid and is one of the most important immune active components in Echinacea purpurea. Echinacea has the functions of enhancing immunity, anti-inflammation, and anti-infection.

The main components of Astragalus are astragaloside IV and astragalus polysaccharide. Astragaloside IV can promote the proliferation and differentiation of lymph node B cells and the formation of plasma cell antibodies. Astragalus polysaccharides can enhance the phagocytosis of phagocytes and increase the weight of spleen and thymus. Astragalus can significantly promote the antibody production function of the normal body. Astragalus has the effects of enhancing immunity, enhancing energy, anti-fatigue, anti-mutation, protecting the liver, and inhibiting osteoclasts.

After the ginkgo pulp is extracted by water, the main component polysaccharides are composed of glucose, galactose, fructose and rhamnose, which are α-glycoside bonded peptidoglycans, which have a significant regulating effect on the hypofunction of cellular immunity and humoral immunity of poultry.

The main component of honeysuckle is chlorogenic acid. Honeysuckle can regulate the physiological function of lymphocytes, significantly increase IL-2, regulate the function of suppressed lymphocytes secreting cytokines, and can regulate specific cellular immunity, non-specific cellular immunity and specific humoral immunity. Flos Lonicerae has heat-clearing and toxic substances removing, cools away wind-heat.

The main ingredient of licorice is glycyrrhizic acid. Glycyrrhizic acid plays a role through dual regulation of cellular immunity and humoral immunity. Glycyrrhizic acid can enhance the proliferation and activity of helper T lymphocytes, promote lymphocytes to produce IL-2, IFN-γ, IL-1 and other cytokines, and inhibit the production of IL-4, IL-10, IL-8, etc. It also has anti-complement activity. Licorice has the functions of invigorating the spleen and replenishing qi, clearing heat and detoxification, eliminating phlegm and relieving cough, relieving spasm and relieving pain, and harmonizing the effects of various medicines.

3. Improve poultry weight gain and feed conversion rate, resulting in good economic benefits

With my country's accession to WTO and the improvement of my country's meat food hygiene standards, feed safety is the basic condition to ensure the safety of animal food, and the development of pollution-free green food is also a top priority for livestock and poultry breeding. Immunopotentiator is a new type of feed additive that people generally pay attention to in the process of feed additive from antibiotic type to non-antibiotic type. Some immune enhancers can not only effectively improve animal immunity, but also play a role in disease resistance and growth promotion. Another prominent feature of the traditional Chinese medicine composition for poultry immune growth of the invention is that it can increase the poultry's weight gain and feed conversion rate, and produce good economic benefits.

detailed description:

The present invention is further described by the following examples. It should be understood that these examples are only for the purpose of illustration, and in no way limit the scope of the present invention.

Test example]:

The 1-day-old Hailan brown commercial laying chickens were divided into control group Ⅰ, control group Ⅱ, levamisole group, and 4% traditional Chinese medicine composition group. Immunization without drug group. At the age of 7 days, the Newcastle disease Ⅳ line was immunized, and at the age of 13, the attenuated bursa vaccine was immunized.

Example 1: Detection results of spleen index in different time periods of 4% traditional Chinese medicine composition

Spleen index=spleen weight (mg)/body weight (g). The test results of spleen index at different ages are shown in Table 1. The 4% traditional Chinese medicine composition was significantly ( P< 0.01) higher than that of the control group Ⅰ, Ⅱ and levamisole Group, show that 4% Chinese medicine composition can increase chicken spleen index, has the effect of stimulating chicken spleen development.

Table 1 Detection results of spleen index of 4% traditional Chinese medicine composition at different ages

group 21 days old 28 days old 35 days old 42 days old Control Ⅰ 1.14±0.136 0.88±0.112 1.06±0.067 0.79±0.402 Control II 0.98±0.091 0.84±0.046 0.98±0.125 0.85±0.206 Levamisole group 1.62±0.144 1.40±0.160 1.21±0.132 1.33±0.401 4% traditional Chinese medicine composition group 2.49±0.133 ^** 1.80±0.161 ^** 1.50±0.140 ^** 1.92±0.104 ^**

Example 2: Detection results of bursa index of 4% traditional Chinese medicine composition in different time periods

Bursa index = weight of bursa (mg)/body weight (g). The test results of bursa index at different ages are shown in Table 2. The 4% traditional Chinese medicine composition was significantly ( P< 0.01) higher than that of the control group Groups Ⅰ, Ⅱ and levamisole indicated that the 4% traditional Chinese medicine composition could increase the index of chicken bursa and stimulate the development of chicken bursa.

Table 2 Detection results of bursa index of 4% traditional Chinese medicine composition at different ages

group 21 days old 28 days old 35 days old 42 days old Control Ⅰ 2.31±0.169 3.79±0.144 3.47±0.182 3.31±0.138 Control II 1.88±0.213 3.56±0.214 3.25±0.355 2.78±0.367 Levamisole group 4.28±0.346 4.28±0.346 4.49±0.355 3.33±0.253 4% traditional Chinese medicine composition group 3.50±0.311 ^** 4.94±0.367 ^** 5.34±0.460 ^** 4.27±0.439 ^**

Example 3: Detection results of 4% traditional Chinese medicine composition for NDV-IV antibody

The results of NDV-IV antibody detection at different ages are shown in Table 3. The 4% traditional Chinese medicine composition was significantly higher ( P< 0.01) than the control group I, II and levamisole group, indicating that the 4% traditional Chinese medicine composition can improve chicken Humoral immune function.

Table 3 The detection results of 4% traditional Chinese medicine composition on NDV-Ⅳ antibody at different ages

group 14 days old 21 days old 28 days old 35 days old 42 days old Control Ⅰ 2.2 ±0.63 3.2±0.79 3.5±0.527 2.7±0.48 1.8±0.42 Control II 2.2±0.32 2.4±0.70 2.4±0.70 1.7±0.48 1.2±0.42 Levamisole group 3.76±0.63 6.42±0.82 ^** 6.72±0.53 ^** 7.24±0.32 ^** 6.14±0.71 ^** 4% traditional Chinese medicine composition group 4.17±0.82 ^** 6.51±0.42 ^** 7.09±0.53 ^** 7.68±0.42 ^** 6.68±0.48 ^**

Example 4: Detection results of 4% traditional Chinese medicine composition to IBDV antibody

The results of IBDV antibody detection at different ages are shown in Table 3. The 4% Chinese medicine composition was significantly higher ( P< 0.01) than the control group I, II and levamisole group, indicating that the 4% Chinese medicine composition can improve the humoral immunity of chickens Function.

Table 4 The detection results of 4% traditional Chinese medicine composition to IBDV antibody (log10) at different ages

group 14 days old 21 days old 28 days old 35 days old 42 days old Control Ⅰ 2.2 ±0.63 3.2±0.79 3.5±0.527 2.7±0.48 1.8±0.42 Control II 2.2±0.32 2.4±0.70 2.4±0.70 1.7±0.48 1.2±0.42 Levamisole group 1.8±0.63 3.3±0.82 ^** 3.5±0.53 ^** 3.1±0.32 ^** 2.5±0.71 ^** 4% traditional Chinese medicine composition group 3.3±0.82 ^** 4.2±0.42 ^** 4.5±0.53 ^** 3.8±0.42 ^** 3.7±0.48 ^**

Embodiment 5: the detection result of 4% Chinese medicine composition chicken serum IL-6 content

The detection results of serum IL-6 levels in different day-old chickens are shown in Table 5. The 4% traditional Chinese medicine composition was significantly ( P< 0.01) higher than that in the control group Ⅰ, Ⅱ and levamisole group, indicating that the 4% traditional Chinese medicine composition can increase the level of IL-6 in chickens. IL-6 content.

Table 5 Detection results of 4% traditional Chinese medicine composition on serum IL-6 content of different day-old chickens (pg/mL)

group 21 days old 28 days old 35 days old Control Ⅰ 181.25±14.42 198.13±12.58 152.74±9.25 Control II —— —— —— Levamisole group 212.55±13.20 ^** 223.04±12.03 ^** 198.78±31.51 ^** 4% traditional Chinese medicine composition group 234.64±18.01 ^** 251.77±16.51 ^** 219.46±22.06 ^**

Example 6: Detection results of 4% Chinese medicine composition chicken serum IFN-γ content

The detection results of serum IFN-γ levels in different day-old chickens are shown in Table 6. The 4% traditional Chinese medicine composition was significantly ( P< 0.01) higher than that in the control group Ⅰ, Ⅱ and levamisole group, indicating that the 4% traditional Chinese medicine composition can improve chicken The content of IFN-γ.

Table 6 The detection results of 4% traditional Chinese medicine composition on the serum IFN-γ content of different day-old chickens (ng/L)

group 21 days old 28 days old 35 days old Control Ⅰ 12.11±0.37 17.45±2.42 15.65±1.59 Control II —— —— —— Levamisole group 25.79±3.26 ^** 28.12±3.26 ^** 29.84±5.67 ^** 4% traditional Chinese medicine composition group 29.64±4.09 ^** 31.57±4.09 ^** 28.97±2.93 ^**

Example 7: Detection results of 4% Chinese medicine composition chicken serum IL-4 content

The detection results of serum IL-4 levels in different day-old chickens are shown in Table 7. The 4% traditional Chinese medicine composition was significantly ( P< 0.01) higher than the control group I, II and levamisole group, indicating that the 4% traditional Chinese medicine composition can improve chicken IL-4 content.

Table 7 The detection results of 4% traditional Chinese medicine composition on serum IL-4 content of chickens of different ages (ng/L)

group 21 days old 28 days old 35 days old Control Ⅰ 283.55±38.52 324.81±41.03 251.27±50.37 Control II —— —— —— Levamisole group 328.46±34.23 ^* 378.10±28.19 ^* 303.85±20.66 ^* 4% traditional Chinese medicine composition group 351.18±17.70 ^** 396.73±48.53 ^** 323.04±22.97 ^**

Example 8: Detection results of 4% traditional Chinese medicine composition chicken serum IL-12 content

The detection results of serum IL-12 levels in different day-old chickens are shown in Table 7. The 4% traditional Chinese medicine composition was significantly ( P< 0.01) higher than that of the control group I, II and levamisole group, indicating that the 4% traditional Chinese medicine composition can improve chicken IL-12 content.

Table 8 Detection results of 4% traditional Chinese medicine composition on serum IL-12 content of different day-old chickens (ng/L)

group 21 days old 28 days old 35 days old Control Ⅰ 265.56±17.33 296.57±38.07 265.78±54.27 Control II —— —— —— Levamisole group 345.58±32.70 ^** 437.26±41.84 ^** 348.25±21.59 ^** 4% traditional Chinese medicine composition group 411.51±67.79 ^** 491.59±50.11 ^** 376.82±46.82 ^**

Example 9: Detection results of 4% traditional Chinese medicine composition on weight gain at different ages

The test results of weight gain at different ages are shown in Table 9. The 4% traditional Chinese medicine composition was significantly ( P< 0.01) higher than that of the control group I, II and levamisole group, indicating that the 4% traditional Chinese medicine composition can increase weight gain.

Table 9 Test results of 4% traditional Chinese medicine composition on weight gain at different ages (g)

Example 10: Detection results of 4% traditional Chinese medicine composition on feed-to-meat ratio at different ages

The test results of feed-to-meat ratio at different ages are shown in Table 10. The 4% traditional Chinese medicine composition was significantly ( P< 0.01) higher than that of the control group I, II and levamisole group, indicating that the 4% traditional Chinese medicine composition can improve the feed quality of broilers. Conversion rate.

Table 10 Test results of 4% traditional Chinese medicine composition on feed-to-meat ratio at different ages

Claims (3)

1. one kind, which is used for bird immunity, grows Chinese medicine composition, it is characterized in that, constituted by mass fraction：Echinacea 30~50, Huang Stilbene 15~25, ginkgo pulp 15~25, honeysuckle 8~12, radix glycyrrhizae 8~12.

2. the preparation method according to claim 1 that Chinese medicine composition is grown for bird immunity, it is characterized in that, by quality Number, takes in Echinacea 30~50, the Radix Astragali 15~25, ginkgo pulp 15~25, honeysuckle 8~12, radix glycyrrhizae 8~12, above-mentioned 5 kinds Medicine chopping processing 80-100 mesh, by distilled water immersion 12-18 hour of 10 times of material gross mass, then boils filtering after 2 h, Collect filtrate；Its filter residue adds 5 times of amount distilled water to boil filtering after 2 h, collects filtrate, filtrate merges twice, is condensed into every mL and contains Medicine 1g decoction.

3. it is used for the application that bird immunity grows Chinese medicine composition, its feature according to made from claim 1 and claim 2 It is that Chinese medicine composition is added in birds free water by 4% concentration of water.