CN107254495A - The method that lipase-catalyzed enzymolysis Rice oil prepares diglyceride - Google Patents
The method that lipase-catalyzed enzymolysis Rice oil prepares diglyceride Download PDFInfo
- Publication number
- CN107254495A CN107254495A CN201710693100.0A CN201710693100A CN107254495A CN 107254495 A CN107254495 A CN 107254495A CN 201710693100 A CN201710693100 A CN 201710693100A CN 107254495 A CN107254495 A CN 107254495A
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- Prior art keywords
- lipase
- rice oil
- diglyceride
- catalyzed
- ultrasonic
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 30
- 235000009566 rice Nutrition 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 15
- 240000007594 Oryza sativa Species 0.000 title 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 40
- 241000209094 Oryza Species 0.000 claims abstract description 29
- 235000011187 glycerol Nutrition 0.000 claims abstract description 20
- 102000004882 Lipase Human genes 0.000 claims abstract description 12
- 108090001060 Lipase Proteins 0.000 claims abstract description 12
- 239000004367 Lipase Substances 0.000 claims abstract description 12
- 235000019421 lipase Nutrition 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 238000006243 chemical reaction Methods 0.000 claims abstract description 11
- 239000000463 material Substances 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 8
- 238000006555 catalytic reaction Methods 0.000 claims abstract description 7
- 239000000523 sample Substances 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000012295 chemical reaction liquid Substances 0.000 claims description 7
- 230000002687 intercalation Effects 0.000 claims description 7
- 238000009830 intercalation Methods 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 6
- 239000007795 chemical reaction product Substances 0.000 claims description 5
- 238000002604 ultrasonography Methods 0.000 claims 1
- 239000003054 catalyst Substances 0.000 abstract description 2
- 238000005265 energy consumption Methods 0.000 abstract description 2
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 230000035484 reaction time Effects 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 235000021472 generally recognized as safe Nutrition 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000019871 vegetable fat Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6454—Glycerides by esterification
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of method that lipase-catalyzed enzymolysis Rice oil prepares diglyceride, comprise the following steps：1) pretreatment of raw material：Fresh Rice oil is taken to be well mixed with glycerine, it is 1 to control mol ratio:1~4；2) 1% 6%TLIM lipase is added in the material of step (1), it is placed in water bath with thermostatic control, utilize ultrasonic probe, ultrasonic temperature at 40~60 DEG C, ultrasonically treated 5~20min under 100~300W ultrasonic power, it is then transferred into water-bath constant temperature oscillator, with 150~170r/min stir speed (S.S.), 4~6h is reacted at a temperature of 40~80 DEG C；3) after reaction terminates, centrifuged under conditions of 4200~4800r/min 15~20 minutes and remove glycerine, obtain being rich in diglyceride product.The present invention is using Rice oil and glycerine as raw material, catalyst is used as using TLIM lipase, ultrasonic wave and enzymic catalytic reaction are effectively combined to prepare diglyceride, reaction condition is gentle, energy consumption is low, environmental pollution is few, selectivity is high, and the speed and yield of reaction are improved, substantially reduce the reaction time.
Description
Technical field
The present invention relates to Rice oil deep process technology field, it is related to a kind of method for preparing diglyceride, and in particular to one
Plant the method that lipase-catalyzed enzymolysis Rice oil prepares diglyceride.
Background technology
Diglyceride is that occur the class formation lipid obtained after esterification by two hydroxyls in glycerine and aliphatic acid,
It is the natural component of vegetable fat and the endogenous intermediate product of body fat mass in human body metabolism, is the food composition of generally recognized as safe, simultaneously
Also it is used widely as emulsifying agent in association areas such as food.
Rice oil is as the raw material for preparing diglyceride, and unsaturated fatty acid content is higher in Rice oil, account for 80%~
85%, also containing the composition such as vitamin and active lipase, these compositions have reducing blood lipid to human body and prevent cardiovascular and cerebrovascular
A variety of alimentary health-care functions such as disease, are also described as " nutrient source being bestowed by heaven ".Moreover, accessory substance of the rice as Rice producing,
Wide material sources, so being conducive to large-scale industrialization production as the raw material for preparing diglyceride from Rice oil, with one
Fixed realistic meaning.Meanwhile, TLIM lipase has been applied in China's food industry at present, but because it is studied not
It is enough, still it is in the junior stage, so diglyceride product is obtained with enzyme by the use of TLIM lipase as reaction, to TLIM lipase
Preferable scientific basis is provided applied to food industry production.
The content of the invention
It is an object of the invention to provide a kind of method that lipase-catalyzed enzymolysis Rice oil prepares diglyceride.
The purpose of the present invention can be achieved through the following technical solutions：
A kind of method that lipase-catalyzed enzymolysis Rice oil prepares diglyceride, comprises the following steps and process conditions：
1), pretreatment of raw material：Take fresh Rice oil to be well mixed with glycerine and obtain mixed material, the Rice oil raw material with
The mol ratio of glycerine is 1:1~4；
2), in step 1) 1%-6%TLIM lipase is added in obtained mixed material, it is placed in water bath with thermostatic control, utilizes
Under ultrasonic probe intercalation reaction liquid, ultrasonic temperature at 40~60 DEG C, ultrasonically treated 5 under 100~300W ultrasonic power~
20min, is then transferred into water-bath constant temperature oscillator, with 150~170r/min stir speed (S.S.), at a temperature of 40~80 DEG C
React 4~6h；
3) after, reaction terminates, by the enzymic catalytic reaction and dewater treatment carried out, collecting reaction product, 4200 simultaneously
Centrifuged under conditions of~4800r/min 15~20 minutes and remove glycerine, obtain being rich in diglyceride product.
Further, the stir speed (S.S.) of the step (2) is 150r/min.
Further, under the ultrasonic probe intercalation reaction liquid at 4~6mm.
Further, the ultrasonic temperature is 50 DEG C.
Further, the ultrasonic power is 250W.
Further, the ultrasonic time is 5min.
Beneficial effects of the present invention：The present invention is using Rice oil and glycerine as raw material, using TLIM lipase as catalyst,
Ultrasonic wave and enzymic catalytic reaction are effectively combined to prepare diglyceride, reaction condition is gentle, energy consumption is low, environmental pollution is few,
Selectivity is high, and improves the speed and yield of reaction, substantially reduces the reaction time.
Embodiment
The present invention is described in further detail with reference to specific embodiment.
Embodiment 1：
First, fresh Rice oil is taken to be well mixed with glycerine in conical flask, according to the mol ratio of Rice oil raw material and glycerine
For 1:2；
2nd, 1%TLIM lipase is added in the mixed material of step one, conical flask is placed in water bath with thermostatic control, is utilized
Under ultrasonic probe intercalation reaction liquid, the ultrasonic temperature at 40 DEG C, ultrasonically treated 5min under 100W ultrasonic power are then transferred into
In water-bath constant temperature oscillator, with 150r/min stir speed (S.S.), 4h is reacted at a temperature of 40 DEG C；
3rd, reaction terminate after, by simultaneously carry out enzymic catalytic reaction and dewater treatment, collecting reaction product,
Centrifuged under conditions of 4200r/min 15 minutes and remove glycerine, obtain being rich in diglyceride product.
Embodiment 2：
First, fresh Rice oil is taken to be well mixed with glycerine in conical flask, according to the mol ratio of Rice oil raw material and glycerine
For 1:3；
2nd, 1%-6%TLIM lipase is added in the mixed material of step one, conical flask is placed in water bath with thermostatic control,
Using under ultrasonic probe intercalation reaction liquid, the ultrasonic temperature at 50 DEG C, ultrasonically treated 15min under 200W ultrasonic power, then
It is transferred in water-bath constant temperature oscillator, with 160r/min stir speed (S.S.), 5h is reacted at a temperature of 60 DEG C；
3rd, reaction terminate after, by simultaneously carry out enzymic catalytic reaction and dewater treatment, collecting reaction product,
Centrifuged under conditions of 4500r/min 20 minutes and remove glycerine, obtain being rich in diglyceride product.
Embodiment 3：
First, fresh Rice oil is taken to be well mixed with glycerine in conical flask, the mol ratio of the Rice oil raw material and glycerine is
1:3；
2nd, 5%TLIM lipase is added in the mixed material of step one, conical flask is placed in water bath with thermostatic control, is utilized
Under ultrasonic probe intercalation reaction liquid, then the ultrasonic temperature at 50 DEG C, ultrasonically treated 20min under 300W ultrasonic power shift
Into water-bath constant temperature oscillator, with 170r/min stir speed (S.S.), 6h is reacted at a temperature of 80 DEG C；
3rd, reaction terminate after, by simultaneously carry out enzymic catalytic reaction and dewater treatment, collecting reaction product,
Centrifuged under conditions of 4800r/min 20 minutes and remove glycerine, obtain being rich in diglyceride product.
Above content is only citing made for the present invention and explanation, and affiliated those skilled in the art are to being retouched
The specific embodiment stated is made various modifications or supplement or substituted using similar mode, without departing from the design of invention
Or surmount scope defined in the claims, protection scope of the present invention all should be belonged to.
Claims (6)
1. a kind of method that lipase-catalyzed enzymolysis Rice oil prepares diglyceride, it is characterised in that comprise the following steps：
1), pretreatment of raw material：Take fresh Rice oil to be well mixed with glycerine and obtain mixed material, the Rice oil raw material and glycerine
Mol ratio be 1:1~4；
2), in step 1) 1%-6%TLIM lipase is added in obtained mixed material, it is placed in water bath with thermostatic control, utilizes ultrasound
Pop one's head under intercalation reaction liquid, the ultrasonic temperature at 40~60 DEG C, ultrasonically treated 5~20min under 100~300W ultrasonic power,
It is then transferred into water-bath constant temperature oscillator, with 150~170r/min stir speed (S.S.), 4 is reacted at a temperature of 40~80 DEG C
~6h；
3) after, reaction terminates, by the enzymic catalytic reaction and dewater treatment carried out simultaneously, collecting reaction product, 4200~
Centrifuged under conditions of 4800r/min 15~20 minutes and remove glycerine, obtain being rich in diglyceride product.
2. the method that a kind of lipase-catalyzed enzymolysis Rice oil according to claim 1 prepares diglyceride, its feature exists
In the stir speed (S.S.) of the step (2) is 150r/min.
3. the method that a kind of lipase-catalyzed enzymolysis Rice oil according to claim 1 prepares diglyceride, its feature exists
In under the ultrasonic probe intercalation reaction liquid at 4~6mm.
4. the method that a kind of lipase-catalyzed enzymolysis Rice oil according to claim 1 prepares diglyceride, its feature exists
In the ultrasonic temperature is 50 DEG C.
5. the method that a kind of lipase-catalyzed enzymolysis Rice oil according to claim 1 prepares diglyceride, its feature exists
In the ultrasonic power is 250W.
6. the method that a kind of lipase-catalyzed enzymolysis Rice oil according to claim 1 prepares diglyceride, its feature exists
In the ultrasonic time is 5min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710693100.0A CN107254495A (en) | 2017-08-14 | 2017-08-14 | The method that lipase-catalyzed enzymolysis Rice oil prepares diglyceride |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710693100.0A CN107254495A (en) | 2017-08-14 | 2017-08-14 | The method that lipase-catalyzed enzymolysis Rice oil prepares diglyceride |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107254495A true CN107254495A (en) | 2017-10-17 |
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| CN201710693100.0A Pending CN107254495A (en) | 2017-08-14 | 2017-08-14 | The method that lipase-catalyzed enzymolysis Rice oil prepares diglyceride |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109837135A (en) * | 2017-11-28 | 2019-06-04 | 中国科学院青岛生物能源与过程研究所 | A kind of method of the rice bran oil enzyme process depickling of ultrasonic wave added |
| CN111235190A (en) * | 2020-01-22 | 2020-06-05 | 新疆农业大学 | Horse oil rich in diglyceride and preparation method thereof |
| CN113913291A (en) * | 2021-11-04 | 2022-01-11 | 广州美林生态科技有限公司 | Shaker ultrasonic device, method for preparing diglyceride from tea oil catalyzed by ultrasonic supercritical enzyme |
| CN118120829A (en) * | 2024-05-08 | 2024-06-04 | 东晓生物科技股份有限公司 | Rice oil diglyceride oil powder grease and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102008078A (en) * | 2010-10-12 | 2011-04-13 | 安徽农业大学 | Method for preparing 1,3-diglyceride-containing milk fat in solvent-free system with all-enzymatic method |
| CN106148439A (en) * | 2016-09-08 | 2016-11-23 | 东北农业大学 | A kind of method that power ultrasonic pretreatment enzyme law catalysis Adeps Sus domestica glycerol rhizolomy prepares diglyceride |
-
2017
- 2017-08-14 CN CN201710693100.0A patent/CN107254495A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102008078A (en) * | 2010-10-12 | 2011-04-13 | 安徽农业大学 | Method for preparing 1,3-diglyceride-containing milk fat in solvent-free system with all-enzymatic method |
| CN106148439A (en) * | 2016-09-08 | 2016-11-23 | 东北农业大学 | A kind of method that power ultrasonic pretreatment enzyme law catalysis Adeps Sus domestica glycerol rhizolomy prepares diglyceride |
Non-Patent Citations (1)
| Title |
|---|
| 陈晓慧等: "超临界体系酶催化制备甘油二酯及其纯化", 《食品科学》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109837135A (en) * | 2017-11-28 | 2019-06-04 | 中国科学院青岛生物能源与过程研究所 | A kind of method of the rice bran oil enzyme process depickling of ultrasonic wave added |
| CN111235190A (en) * | 2020-01-22 | 2020-06-05 | 新疆农业大学 | Horse oil rich in diglyceride and preparation method thereof |
| CN111235190B (en) * | 2020-01-22 | 2023-11-07 | 新疆农业大学 | Horse oil rich in diglyceride and preparation method thereof |
| CN113913291A (en) * | 2021-11-04 | 2022-01-11 | 广州美林生态科技有限公司 | Shaker ultrasonic device, method for preparing diglyceride from tea oil catalyzed by ultrasonic supercritical enzyme |
| CN118120829A (en) * | 2024-05-08 | 2024-06-04 | 东晓生物科技股份有限公司 | Rice oil diglyceride oil powder grease and preparation method and application thereof |
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Application publication date: 20171017 |
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