CN107156156A - A kind of bactericidal composition - Google Patents
A kind of bactericidal composition Download PDFInfo
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- CN107156156A CN107156156A CN201710339280.2A CN201710339280A CN107156156A CN 107156156 A CN107156156 A CN 107156156A CN 201710339280 A CN201710339280 A CN 201710339280A CN 107156156 A CN107156156 A CN 107156156A
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- China
- Prior art keywords
- pyraclostrobin
- platycodon root
- root polysaccharide
- bactericidal composition
- platycodon
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- 239000000203 mixture Substances 0.000 title claims abstract description 42
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 25
- 239000005869 Pyraclostrobin Substances 0.000 claims abstract description 55
- HZRSNVGNWUDEFX-UHFFFAOYSA-N pyraclostrobin Chemical compound COC(=O)N(OC)C1=CC=CC=C1COC1=NN(C=2C=CC(Cl)=CC=2)C=C1 HZRSNVGNWUDEFX-UHFFFAOYSA-N 0.000 claims abstract description 55
- 235000006751 Platycodon Nutrition 0.000 claims abstract description 54
- 150000004676 glycans Chemical class 0.000 claims abstract description 54
- 229930189914 platycodon Natural products 0.000 claims abstract description 54
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 54
- 239000005017 polysaccharide Substances 0.000 claims abstract description 54
- 244000274050 Platycodon grandiflorum Species 0.000 claims abstract description 44
- 239000004480 active ingredient Substances 0.000 claims abstract description 16
- 241000221785 Erysiphales Species 0.000 claims abstract description 13
- 241000233622 Phytophthora infestans Species 0.000 claims abstract description 11
- 239000004562 water dispersible granule Substances 0.000 claims description 8
- 239000000375 suspending agent Substances 0.000 claims description 6
- 244000241257 Cucumis melo Species 0.000 claims 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 claims 1
- 240000008067 Cucumis sativus Species 0.000 abstract description 17
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 abstract description 17
- 230000000694 effects Effects 0.000 abstract description 13
- 230000002195 synergetic effect Effects 0.000 abstract description 7
- 239000013043 chemical agent Substances 0.000 abstract description 6
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- 238000012360 testing method Methods 0.000 description 17
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- 238000000034 method Methods 0.000 description 11
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- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 6
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- 229910019142 PO4 Inorganic materials 0.000 description 3
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- 239000003899 bactericide agent Substances 0.000 description 3
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- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 2
- 239000005995 Aluminium silicate Substances 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 235000012211 aluminium silicate Nutrition 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
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- 230000033228 biological regulation Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
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- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 239000002574 poison Substances 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 229940051841 polyoxyethylene ether Drugs 0.000 description 2
- 229920000056 polyoxyethylene ether Polymers 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 229920002545 silicone oil Polymers 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 231100000820 toxicity test Toxicity 0.000 description 2
- 241000235349 Ascomycota Species 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 108010004032 Bromelains Proteins 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000675108 Citrus tangerina Species 0.000 description 1
- 102100025287 Cytochrome b Human genes 0.000 description 1
- 108010075028 Cytochromes b Proteins 0.000 description 1
- 108090000270 Ficain Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 239000005800 Kresoxim-methyl Substances 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- 240000008790 Musa x paradisiaca Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 description 1
- 241000233654 Oomycetes Species 0.000 description 1
- 241000233679 Peronosporaceae Species 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000007798 antifreeze agent Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 235000019835 bromelain Nutrition 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 108010035432 cytochrome C(L) Proteins 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 235000019836 ficin Nutrition 0.000 description 1
- POTUGHMKJGOKRI-UHFFFAOYSA-N ficin Chemical compound FI=CI=N POTUGHMKJGOKRI-UHFFFAOYSA-N 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- ZOTBXTZVPHCKPN-HTXNQAPBSA-N kresoxim-methyl Chemical compound CO\N=C(\C(=O)OC)C1=CC=CC=C1COC1=CC=CC=C1C ZOTBXTZVPHCKPN-HTXNQAPBSA-N 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 229920005646 polycarboxylate Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of bactericidal composition, active ingredient is made up of platycodon root polysaccharide and pyraclostrobin, and the weight ratio of platycodon root polysaccharide and pyraclostrobin is 15 1:1‑20.The bactericidal composition that the present invention carries out mixture by platycodon root polysaccharide and pyraclostrobin is used to prevent and treat powdery mildew of cucumber and the late blight of potato, synergistic function is obvious, platycodon root polysaccharide as pyraclostrobin synergist, inhibitory activity of the pyraclostrobin to germ can be significantly improved, the two mixture can also reduce the use of chemical agent pyraclostrobin, reduce the residual of chemical agent.Both mixtures can also delay the drug-fast generation of germ simultaneously, improve prevention effect.
Description
Technical field
The present invention relates to pesticides application technical field, and in particular to a kind of bactericidal composition, and particularly one kind is used for
Agriculturally prevent and treat the bactericidal composition of powdery mildew of cucumber and the late blight of potato.
Background technology
Platycodon root polysaccharide is one of balloonflower root (Platycodon grandiflorus) principle active component.Modern pharmacology is ground
Studying carefully discovery platycodon root polysaccharide has an a variety of bioactivity, such as immunological regulation, antitumor, anti-oxidant, Eradicates phlegm, anti-inflammatory, liver protection, anti-ageing
Always, the effect such as enhancing body's immunity and improvement insulin, therefore, for the research of platycodon root polysaccharide, by with very high medicine
With value.Chinese patent 201410628520.7 discloses a kind of extracting method of platycodon root polysaccharide, including step：What selection was dried
Balloonflower root, crosses 40-80 mesh sieves, obtains balloonflower powder, add water and stir in balloonflower powder after crushing, the part by weight of balloonflower powder and water
For 1:5-10, soaks 8-16 hours in water, adds cellulase degradation, adds ficin and bromelain,
Digested under microwave condition, concentrate and decolourize after enzymolysis, centrifuged, take supernatant concentration alcohol precipitation, then centrifuged, clean heavy using ethanol
Form sediment, after freeze-drying and crush, obtain platycodon root polysaccharide.At present, the antibacterial and Study of Antioxidation on platycodon root polysaccharide is rarely reported.
What Yang Xiaojie, Dong Yanan, Li Na of Qiqihar University et al. were delivered《The biocidal property and Study of Antioxidation of platycodon root polysaccharide》Card
Real platycodon root polysaccharide has bacteriostatic activity to bread mold, mould etc..
Pyraclostrobin (English common name：), also known as pyraclostrobin, hectogram are quick, molecular formula Pyraclostrobin：
C19H18ClN3O4.The bactericide is that the methoxy acrylic for having pyrrazole structure concurrently developed by BASF Aktiengesellschaft is killed
Microbial inoculum, can effectively prevent and treat the crop disease as caused by Ascomycetes, Basidiomycetes, Fungi Imperfecti and Oomycete fungal.This is killed
The mechanism of action of microbial inoculum is mitochondrial respiratory inhibitor.Suppress line grain by preventing electron transmission between cytochrome b and cl
Body respiration, prevents energy (ATP) of the mitochondria required for from producing and providing cell eubolism, ultimately results in cell dead
Die.With protection, treatment, blade infiltration conduction.Pyraclostrobin shows to cucumber white powder through field control effectiveness test result
Sick, downy mildew and banana freckle, leaf spot, sclerotiniose etc. have preferable prevention effect.
Used however, either pyraclostrobin or other bactericide are single for a long time, inevitably produce germ
The resistance to the action of a drug.Therefore the active ingredient of the different mechanisms of action is compounded, is to delay disease to develop immunity to drugs effective side
One of method.At present using platycodon root polysaccharide, pyraclostrobin as active ingredient composition be applied to powdery mildew, late blight research still
There is not relevant report.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of bactericidal composition, can significantly improve the suppression of pyraclostrobin
Bacterium activity, reduces chemical pesticide dosage, delays the drug-fast generation of germ.
The present invention is to be achieved through the following technical solutions：A kind of bactericidal composition, active ingredient is by platycodon root polysaccharide and pyrazoles
Kresoxim-methyl is constituted, and the weight ratio of platycodon root polysaccharide and pyraclostrobin is 15-1:1-20.Preferably, platycodon root polysaccharide and pyrazoles ether bacterium
The weight ratio of ester is 10-1:1-10.
Described bactericidal composition adds what corresponding auxiliary element was made according to method known in those skilled in the art
Formulation is water dispersible granules or suspending agent.
Described bactericidal composition is applied to prevent and treat powdery mildew of cucumber and the late blight of potato.
The auxiliary element that bactericidal composition of the present invention is used include dispersant, wetting agent, binding agent, disintegrant, antifreezing agent,
Defoamer, thickener, filler, solvent etc. or it is other be beneficial to active ingredient in the formulation stablize and drug effect play known substance
Matter, is all the various composition commonly used in field of pesticide preparation or allow to use.
Beneficial effect of the present invention：The bactericidal composition that the present invention carries out mixture by platycodon root polysaccharide and pyraclostrobin is used for
Prevent and treat powdery mildew of cucumber and the late blight of potato, synergistic function substantially, platycodon root polysaccharide as pyraclostrobin synergist,
Inhibitory activity of the pyraclostrobin to germ can be significantly improved, the two mixture can also reduce chemical agent pyraclostrobin
Use, reduce the residual of chemical agent.Both mixtures can also delay the drug-fast generation of germ simultaneously, improve prevention effect.
Embodiment
Technical scheme and technique effect are described further below in conjunction with specific embodiment, institute of the present invention
The percentage stated is weight percentage.
First, Toxicity Determination is tested
In order to illustrate synergistic effect of the platycodon root polysaccharide for pyraclostrobin, the present invention calculates poison system altogether using the abundant methods of Sun Yun
(CTC) mixed effect is evaluated.
Toxicity index TI (A)=(EC50 of standard agent A EC50 ÷ A agent) × 100
Toxicity index TI (B)=(EC50 of standard agent A EC50 ÷ B agent) × 100
Actual toxicity index ATI (AB)=(A EC50 ÷ AB EC50) × 100
Percentage+TI (B) × B percentages in mixture of theoretical toxicity index TTI (AB)=TI (the A) × A in mixture
Number
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI) ÷ mixtures theoretical toxicity index (TTI] × 100
Evaluation criterion is：Co-toxicity coefficient >=120 show as synergistic effect；Co-toxicity coefficient≤80 show as antagonism；80<
Co-toxicity coefficient<120 show as summation action.
1. indoor joint toxicity measuring test 1
Test method：Reference《The parts of farm-chemical indoor determination test rule NY/T 1156.13-2008 the 13rd：Suppress
Late disease bacteria pilot blade method》, handled with the reagent spray for preparing respective concentration after potato healthy leaves, 24h, with preparation
Good spore suspension even spraying, cultivates 5-7d under suitable humiture and illumination condition, waits the sick leaf rate of blank control to reach
During to more than 50%, incidence is managed in classification investigation everywhere, calculates the EC50 of disease index and each medicament, and according to the abundant methods of Sun Yun
Calculate the co-toxicity coefficient of mixture.
The Toxicity Determination result counted according to above-mentioned computational methods is as shown in table 1：
The toxicity test analysis of results table of the pyraclostrobin of table 1, platycodon root polysaccharide and its compounding to the late blight of potato
| Medicament | EC50(μg/mL) | Survey toxicity index | Theoretical toxicity index | Co-toxicity coefficient |
| Pyraclostrobin single dose (A) | 0.41 | 103677.72 | / | / |
| Platycodon root polysaccharide single dose (B) | 421.55 | 100.00 | / | / |
| A:B=20:1 | 0.35 | 120442.10 | 98745.45 | 122.0 |
| A:B=15:1 | 0.33 | 127741.62 | 97204.12 | 131.4 |
| A:B=10:1 | 0.31 | 135983.01 | 94261.57 | 144.3 |
| A:B=5:1 | 0.22 | 191612.43 | 86414.77 | 221.7 |
| A:B=1:1 | 0.41 | 102816.42 | 51888.86 | 198.1 |
| A:B=1:5 | 1.65 | 25548.32 | 17362.95 | 147.1 |
| A:B=1:10 | 2.87 | 14688.06 | 9516.16 | 154.3 |
| A:B=1:15 | 4.86 | 8673.81 | 6573.61 | 131.9 |
| A:B=1:20 | 7.27 | 5798.45 | 5032.27 | 115.2 |
| A:B=1:30 | 17.56 | 2400.61 | 3441.22 | 69.8 |
| A:B=30:1 | 0.59 | 71448.70 | 100336.51 | 71.2 |
It was found from upper table 1, the weight ratio of pyraclostrobin and platycodon root polysaccharide is in 20-1:In the range of 1-15 during mixture, it is total to
Malicious coefficient is all higher than 120, and obvious synergistic function is shown to the late blight of potato；And when pyraclostrobin and balloonflower root
Many sugar weight ratios are 1:30、30:When 1, its co-toxicity coefficient is less than 80, shows obvious antagonism；Pyraclostrobin and tangerine
It is 1 to obstruct many sugar weight ratios:20 show simple summation action.
2. indoor joint toxicity measuring test 2
Test method：The cucumber seedling for preparing health is potted plant, and all cucumber seedlings are inoculated with the same time using spore method is shaken off
Cucumber powdery mildew's pathogen, after inoculation by cucumber seedling be placed on 18 DEG C, humidity be 70%, light application time be 16h+ interlunations be 8h perseverance
Cultivated in greenhouse.It is inoculated with after 24h and carries out reagent spray processing.(about 7 days left sides when sorus is grown on the cucumber seedling of blank control
It is right) disease index of each processing of investigation, the EC50 of each medicament is calculated, and calculate according to the abundant methods of Sun Yun the co-toxicity coefficient of mixture.
The Toxicity Determination result counted according to above-mentioned computational methods is as shown in table 2 below：
The toxicity test analysis of results table of the pyraclostrobin of table 2, platycodon root polysaccharide and its compounding to powdery mildew of cucumber
| Medicament | EC50(μg/mL) | Survey toxicity index | Theoretical toxicity index | Co-toxicity coefficient |
| Pyraclostrobin single dose (A) | 9.18 | 3897.11 | / | / |
| Platycodon root polysaccharide single dose (B) | 357.61 | 100.00 | / | / |
| A:B=20:1 | 7.14 | 5008.58 | 3716.29 | 134.8 |
| A:B=15:1 | 7.10 | 5036.80 | 3659.79 | 137.6 |
| A:B=10:1 | 5.89 | 6071.53 | 3551.92 | 170.9 |
| A:B=5:1 | 5.72 | 6251.97 | 3264.26 | 191.5 |
| A:B=1:1 | 8.21 | 4355.82 | 1998.55 | 217.9 |
| A:B=1:5 | 26.34 | 1357.68 | 732.85 | 185.3 |
| A:B=1:10 | 51.69 | 691.84 | 445.19 | 155.4 |
| A:B=1:15 | 81.16 | 440.63 | 337.32 | 130.6 |
| A:B=1:20 | 125.75 | 284.38 | 280.81 | 101.3 |
| A:B=30:1 | 9.77 | 3660.32 | 3774.62 | 97.0 |
| A:B=40:1 | 15.81 | 2261.94 | 3804.49 | 59.5 |
It was found from upper table 2, the weight ratio of pyraclostrobin and platycodon root polysaccharide is in 20-1:When in the range of 1-15, its common poison system
Number is all higher than 120, and obvious synergistic function is shown to powdery mildew of cucumber；And when pyraclostrobin and platycodon root polysaccharide weight
Amount is than being 40:When 1, its co-toxicity coefficient is less than 80, shows obvious antagonism；Pyraclostrobin and platycodon root polysaccharide weight ratio
For 1:20、30:1 shows simple summation action.
2nd, product prepares embodiment
Embodiment 1：30% platycodon root polysaccharide pyraclostrobin water dispersible granule (1:5)
Platycodon root polysaccharide (active ingredient) 5%, pyraclostrobin (active ingredient) 25%, polycarboxylate (dispersant) 6.5%,
Calcium dodecyl benzene sulfonate (wetting agent) 5.5%, starch (binding agent) 3%, sodium carbonate (disintegrant) 2.5%, kaolin (are filled out
Material) complement to 100%；Each component is well mixed, after being crushed through airslide disintegrating mill, 30min is stirred, mediates, then add stream
Change and granulated in bed, be dried after granulation is good, sieve i.e. obtained 30% platycodon root polysaccharide pyraclostrobin water dispersible granule.
Embodiment 2：33% platycodon root polysaccharide pyraclostrobin water dispersible granule (10:1)
Platycodon root polysaccharide (active ingredient) 30%, pyraclostrobin (active ingredient) 3%, aliphatic alcohol polyoxyvinethene phosphate
(dispersant) 6%, lauryl sodium sulfate (wetting agent) 5.5%, polyvinyl alcohol (binding agent) 3%, sodium carbonate (disintegrant)
2.5%th, diatomite (filler) 9%, kaolin (filler) complement to 100%；Each component is well mixed, through airslide disintegrating mill powder
After broken, stirring 30min is mediated, and is then added and is granulated in fluid bed, is dried after granulation is good, is sieved i.e. obtained 33%
Platycodon root polysaccharide pyraclostrobin water dispersible granule.
Embodiment 3：22% platycodon root polysaccharide pyraclostrobin suspending agent (1:10)
Platycodon root polysaccharide (active ingredient) 2%, pyraclostrobin (active ingredient) 20%, pull open powder (wetting agent) 3%, fat
Alcohol polyoxyethylene ether phosphate (dispersant) 5%, xanthans (thickener) 2.5%, ethylene glycol (antifreezing agent) 3%, organic silicone oil
(defoamer) 0.5%, deionized water (solvent) complement to 100%；First other auxiliary agents are mixed, shear-mixed is equal in cutter
It is even, platycodon root polysaccharide and pyraclostrobin are then added, 2h is ground in ball crusher, you can 22% platycodon root polysaccharide pyrazoles ether is made
Bacterium ester suspending agent.
Embodiment 4：16% platycodon root polysaccharide pyraclostrobin suspending agent (1:15)
Platycodon root polysaccharide (active ingredient) 1%, pyraclostrobin (active ingredient) 15%, calcium dodecyl benzene sulfonate (moistening
Agent) 3.5%, aliphatic alcohol polyoxyvinethene phosphate (dispersant) 3.5%, Magnesiumaluminumsilicate (thickener) 2%, ethylene glycol it is (antifreeze
Agent) 3%, organic silicone oil (defoamer) 0.3%, deionized water (solvent) complement to 100%；First other auxiliary agents are mixed, cut
Cut shear-mixed in machine uniform, then add platycodon root polysaccharide and pyraclostrobin, 2h is ground in ball crusher, you can be made 16%
Platycodon root polysaccharide pyraclostrobin suspending agent.
Embodiment 5：44% platycodon root polysaccharide pyraclostrobin water dispersible granule (10:1)
Platycodon root polysaccharide (active ingredient) 40%, pyraclostrobin (active ingredient) 4%, styrylphenol polyoxyethylene ether
(dispersant) 6.5%, calcium dodecyl benzene sulfonate (wetting agent) 6%, sodium carboxymethylcellulose (binding agent) 3%, sodium carbonate (collapse
Solution agent) 3%, diatomite (filler) complement to 100%；Each component is well mixed, after being crushed through airslide disintegrating mill, stirring
30min, is mediated, and is then added and is granulated in fluid bed, is dried after granulation is good, is sieved i.e. obtained 44% platycodon root polysaccharide
Pyraclostrobin water dispersible granule.
3rd, crop field test examples
Disease index and prevention effect computational methods：
Disease index calculation formula：
In formula：
X --- disease index；
Ni--- the sick numbers of sheets at different levels；
I --- with respect to value of series；
N --- investigation total number of sheets.
Prevention effect formula：
In formula：
P --- prevention effect, unit is percentage (%)；
CK0--- disease index before blank district dispenser；
CK1--- disease index after blank district dispenser；
PT0--- disease index before chemicals treatment district dispenser；
PT1--- disease index after chemicals treatment district dispenser.
1. field medicine effect test 1
Test method：Reference《The part of GB/T17980.34-2000 pesticide field efficacy medicine tests criterion (one) the 34th：Sterilization
Agent prevents and treats the late blight of potato》Test method.Second of spray pesticide after first time spray pesticide at the initial stage of a disease, 7d, altogether
Dispenser is twice.Experiment sets 6 processing altogether, often handles 4 repetitions, and each plot area is 46m2, cell processing is using random area
Group arrangement.Test site：Guangxi Guigang.
Investigation method：Before dispenser, 7 days after second of dispenser, each cell is investigated within 15 days, each cell carries out five point samplings
Investigation, every takes continuous 3 plants, looks into whole blades, and grade scale is investigated according to related data, and record is classified in units of blade
Incidence, calculates disease index and prevents and treats preventive effect.
Prevent and treat the field control effectiveness test result such as table 3 below of the late blight of potato：
The platycodon root polysaccharide of table 3, pyraclostrobin and its compounding prevent and treat the late blight of potato test of pesticide effectiveness
Show that platycodon root polysaccharide and the bactericidal composition of pyraclostrobin compounding can effectively prevent and treat potato by the result of the test of table 3
Late blight, preventive effect is significantly higher than the preventive effect of alone platycodon root polysaccharide and pyraclostrobin.
2. field medicine effect test 2
Test method：Powdery mildew of cucumber field control effectiveness test is prevented and treated according to medicine inspecting institute of the Ministry of Agriculture " GB/T17980.30-2000
The regulation of pesticide field efficacy medicine test criterion (one)-bactericidal agent for preventing and treating powdery mildew of cucumber ", experiment sets 6 processing altogether, often handles 4
Secondary repetition, plot area 40m2, 4 repetitions, cell processing is using the arrangement of random district's groups.Spraying is applied for the first time at the initial stage of a disease
Second of spray pesticide after medicine, 7d, common dispenser is twice.Test site：Guangxi Guigang.
Investigation method：Before dispenser, 7 days after second of dispenser, 5 sampling surveys are carried out in each cell respectively within 15 days,
Every takes continuous 2 plants, looks into whole blades, and grade scale is investigated according to related data, the classification record morbidity in units of blade
Situation, calculates disease index and preventive effect, and statistical result is as shown in table 4 below.
The platycodon root polysaccharide of table 4, pyraclostrobin and its compounding prevent and treat the powdery mildew of cucumber test of pesticide effectiveness
Showing the bactericidal composition of platycodon root polysaccharide and pyraclostrobin compounding by the result of the test of table 4, effectively to prevent and treat cucumber white
Powder disease, preventive effect is significantly higher than the preventive effect of alone platycodon root polysaccharide and pyraclostrobin.
In summary, the bactericidal composition that the present invention carries out mixture by platycodon root polysaccharide and pyraclostrobin is applied to preventing and treating
Powdery mildew of cucumber and the late blight of potato, substantially, the two mixture can also reduce chemical agent pyrazoles ether bacterium to synergistic function
The use of ester, reduces the residual of chemical agent.Both mixtures can also delay the drug-fast generation of germ simultaneously, improve preventing and treating effect
Really.Bactericidal composition of the present invention is equal to trial crops without obvious harmful effect, and security is good, and the security for meeting pesticidal preparations will
Ask.
Claims (4)
1. a kind of bactericidal composition, it is characterised in that active ingredient is made up of platycodon root polysaccharide and pyraclostrobin, platycodon root polysaccharide and
The weight ratio of pyraclostrobin is 15-1:1-20.
2. bactericidal composition according to claim 1, it is characterised in that the weight ratio of platycodon root polysaccharide and pyraclostrobin is
10-1:1-10。
3. bactericidal composition according to claim 1 or 2, it is characterised in that described bactericidal composition can be prepared into water
Dispersible granule or suspending agent.
4. bactericidal composition according to claim 1 or 2, it is characterised in that described bactericidal composition is used for anti-Yellow River harnessing
Melon powdery mildew and the late blight of potato.
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| CN112825860A (en) * | 2021-01-23 | 2021-05-25 | 青岛海大汇信生物科技有限公司 | Bactericidal regulator containing alginate oligosaccharides and pyraclostrobin and preparation method thereof |
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|---|---|---|---|---|
| CN104255756A (en) * | 2014-09-10 | 2015-01-07 | 江苏省绿盾植保农药实验有限公司 | Bactericidal composition containing pyraclostrobin and chitosan, and application thereof |
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| CN104255756A (en) * | 2014-09-10 | 2015-01-07 | 江苏省绿盾植保农药实验有限公司 | Bactericidal composition containing pyraclostrobin and chitosan, and application thereof |
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| 杨晓杰等: "桔梗多糖的抑菌性和抗氧化性研究", 《食品研究与开发》 * |
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| CN112825860A (en) * | 2021-01-23 | 2021-05-25 | 青岛海大汇信生物科技有限公司 | Bactericidal regulator containing alginate oligosaccharides and pyraclostrobin and preparation method thereof |
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