CN107151189A - A kind of selenium-enriched liquid bacterial manure and preparation method and application - Google Patents

Abstract

The invention provides a selenium-enriched liquid bacterial fertilizer and its preparation method and application, belonging to the field of agricultural biotechnology. The raw materials of the selenium-enriched liquid bacterial fertilizer include biogas slurry, mushroom dregs, EM bacterial liquid, EM original bacterial liquid, CM bacteria, selenite, and brown sugar. The selenium-enriched liquid bacterial fertilizer can provide vegetables with various nutrients and promote growth. The preparation method of the selenium-enriched liquid bacterial fertilizer comprises: adding part of the EM bacterial liquid, mushroom dregs, and part of the CM bacterial liquid into the biogas slurry, adjusting the pH value, and performing the first fermentation to obtain the first fermented liquid; EM bacterial liquid, part of the selenite solution is mixed with the first fermentation liquid; after 4-6 days, add part of the EM original bacterial liquid, the remaining CM bacterial liquid and the remaining selenite solution, and carry out the second fermentation to obtain Selenium-enriched liquid bacterial fertilizer. The preparation method has simple technology and can effectively prepare the selenium liquid bacterial fertilizer with excellent performance. The preparation method of the selenium-enriched liquid bacterial fertilizer can be applied to foliage fertilizer or root irrigation fertilizer.

Description

A kind of selenium-enriched liquid bacterial fertilizer and its preparation method and application

technical field

The invention relates to the field of agricultural biotechnology, in particular to a selenium-enriched liquid fertilizer and its preparation method and application.

Background technique

With the continuous development of the economy and the continuous progress of the society, people gradually begin to have higher and higher requirements for the quality of life; they also pay more and more attention to food safety.

At present, organic ingredients are becoming more and more sought after by everyone, and a large amount of organic fertilizer is needed in the process of planting organic ingredients; however, farm organic fertilizer degrades slowly and has low utilization efficiency, which is not conducive to large-scale production and use; the general bacterial fertilizer fermentation cycle Long, which limits the large-scale and rapid production of bacterial fertilizer, which is not conducive to production and application.

Contents of the invention

The first object of the present invention is to provide a selenium-enriched liquid bacterial fertilizer. The selenium-enriched liquid bacterial fertilizer utilizes waste resources such as biogas slurry and mushroom residue for recycling, reduces production costs, and slows down environmental pollution. It can provide vegetables with A variety of nutrient elements can promote growth, can reduce the amount of chemical fertilizers used, have high efficiency, and have low toxicity, which improves the added value of vegetable products.

The second object of the present invention is to provide a method for preparing selenium-enriched liquid bacterial fertilizer. The preparation method is simple in technology and can effectively prepare selenium liquid bacterial fertilizer with excellent performance.

The third object of the present invention is to provide the application of a preparation method of selenium-enriched liquid bacterial fertilizer in foliage fertilizer or root irrigation fertilizer.

In order to realize the above-mentioned purpose of the present invention, adopt following technical scheme:

A selenium-enriched liquid bacterial fertilizer, its raw materials include: 70-85 parts of biogas slurry, 13-20 parts of mushroom dregs, 21-28 parts of EM bacterial liquid, 0.8-1.2 parts of EM original bacterial liquid, CM 3.3-4.7 parts of bacteria, 0.3-0.75 parts of selenite, and 4-6 parts of brown sugar.

A method for preparing selenium-enriched liquid bacterial fertilizer, comprising: adding 20-25 parts of EM bacterial liquid, 13-20 parts of mushroom dregs, and 1.5-2.5 parts of CM bacterial liquid to 70-85 parts by weight In the biogas slurry of 1 part, adjust the pH value to 7.0-7.5, and carry out the first fermentation to obtain the first fermentation liquid; mix 1-3 parts of EM bacterial liquid, 0.1-0.4 part of selenite solution with the first fermentation liquid Mix; carry out stirring and fermentation; after 4-6 days, add 0.4-0.6 parts of EM original bacterial liquid, 1.8-2.2 parts of CM bacterial liquid and 0.2-0.35 parts of selenite solution for second fermentation; obtain selenium-enriched liquid bacteria Fat.

An application of the above-mentioned selenium-enriched liquid bacterial fertilizer preparation method in the preparation of foliage fertilizer or root irrigation fertilizer.

The beneficial effects of a kind of selenium-enriched liquid fertilizer provided by the invention and its preparation method and application are:

The selenium-enriched liquid fertilizer provided by the invention mainly includes biogas slurry, mushroom dregs, EM bacteria liquid, EM original bacteria liquid, CM bacteria, sodium selenite, and brown sugar.

Among them, biogas slurry and mushroom dregs contain a large amount of organic acids, amino acids, nutrient elements, growth hormones and other substances that are beneficial to vegetable growth; brown sugar contains amino acids, cellulose, vitamins and electrolytes, which can regulate the concentration of certain substances between tissues. balance the water metabolism in the intracellular environment, eliminate cell metabolites, and keep the internal and external environment of the cell clean; use the complex biological flora EM, CM and the above organic substances to convert inorganic selenium (sodium selenite) into organic selenium Selenium, forming organic selenium-rich liquid bacterial fertilizer.

The components of the raw materials above play a synergistic role at the same time, and the prepared selenium-enriched liquid bacterial fertilizer has comprehensive and balanced nutrition, which can fully improve vegetable quality and soil ecology. In parts by weight, biogas slurry 70-85 parts, mushroom residue 13-20 parts, EM bacteria liquid 21-28 parts, EM original bacteria liquid 0.8-1.2 parts, CM bacteria 3.3-4.7 parts, selenite 0.3- 0.75 parts, brown sugar 4-6 parts. The ratio is scientifically designed, and the selenium-enriched liquid bacteria prepared from the raw materials prepared according to the ratio can improve vegetable quality and soil ecology. The selenium-enriched liquid bacteria has the effects of labor saving, fertilizer saving, drug saving, land saving, and mechanical cost saving; increasing output, increasing benefit, enhancing disease resistance, and the like.

The preparation method of a kind of selenium-enriched liquid fertilizer provided by the present invention comprises adding 20-25 parts of EM bacteria liquid, 13-20 parts of mushroom dregs, and 1.5-2.5 parts of CM bacteria liquid into 70 parts by weight. -85 parts of biogas slurry, adjust the pH value to 7.0-7.5, carry out the first fermentation to obtain the first fermentation liquid; mix 1-3 parts of EM bacterial liquid, 0.1-0.4 part of selenite solution with the first Mix the fermentation broth; carry out stirring fermentation; after 4-6 days, add 0.4-0.6 parts of EM original bacterial liquid, 1.8-2.2 parts of CM bacterial liquid and 0.2-0.35 parts of selenite solution for the second fermentation; obtain selenium-enriched Liquid bacterial fertilizer; obtain selenium-enriched liquid bacterial fertilizer. The two fermentations are conducive to the full release of the organic matter in the mushroom residue and biogas slurry, which can fully combine with the complex biological flora EM and CM to convert inorganic selenium into organic selenium. The preparation method is simple in technology and can effectively prepare the selenium liquid bacterial fertilizer with excellent performance.

The preparation method of the selenium-enriched liquid bacterial fertilizer provided by the invention can be applied to the preparation of foliage fertilizer or root irrigation fertilizer.

detailed description

Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only for illustrating the present invention, and should not be considered as limiting the scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased from the market.

A selenium-enriched liquid bacterial fertilizer provided in an embodiment of the present invention, its preparation method and application are described in detail below.

Organic liquid fertilizer is a new type of fertilizer with high concentration, high fertilizer efficiency, multi-function and full nutritional synergy. Compared with traditional chemical fertilizers, it can be absorbed by plants in a very short time to avoid loss, and it is simple and convenient to apply, easy to transport and store. It contains a large number of active cells and high-efficiency nutritional enzymes, rich in organic matter, nitrogen, phosphorus, potassium, and various trace elements such as calcium, magnesium, sulfur, boron, zinc, molybdenum, copper, manganese, and iron. It has various nutrients such as aloe essence, amino acid, humic acid and water-soluble protein.

A kind of selenium-enriched liquid bacterial fertilizer provided by the embodiment of the present invention, in parts by weight, its raw materials include: 70-85 parts of biogas slurry, 13-20 parts of mushroom dregs, 21-28 parts of EM bacterial liquid, EM original bacteria 0.8-1.2 parts of liquid, 3.3-4.7 parts of CM bacteria, 0.3-0.75 parts of selenite, and 4-6 parts of brown sugar.

Among them, the selection and proportioning of each raw material component is scientifically designed so that the interaction between various raw materials can not only maximize the mutual reaction but also play a synergistic effect. Using this proportioning, the activity of various raw materials The interaction and supplementation of ingredients are more conducive to fully improving the quality of vegetables and improving soil ecology.

Preferably, in order to further improve vegetable quality and soil ecology, in parts by weight, biogas slurry is 70-85 parts, mushroom residue is 13-20 parts, EM bacteria is 0.8-1.2 parts, and CM bacteria is 1.5-2.5 parts parts, sodium selenite is 0.2-0.4 parts, brown sugar is 4-6 parts; within this range, most preferably, by weight parts, biogas slurry is 75 parts, mushroom dregs is 16 parts, EM bacteria is 1 When CM bacteria was 2 parts, sodium selenite was 0.3 parts, and brown sugar was 5 parts, the effect of improving vegetable quality and soil ecology was the best.

The selenium-enriched liquid bacterial fertilizer provided by the invention comprises the following raw materials: biogas slurry, mushroom dregs, EM bacteria, CM bacteria, sodium selenite, and brown sugar.

Among them, EM (Effective Microorganisms, EM) bacterial liquid means effective microbial group bacterial liquid, which is composed of photosynthetic bacteria, lactic acid bacteria group, yeast group, actinomycete group, filamentous group and more than 80 kinds of microorganisms in 10 genera. Composed of; EM technology is currently the world's largest application of a bioengineering technology. Compared with general biological agents, it has the advantages of complex structure, stable performance and complete functions, showing an unprecedented high-tech level. Moreover, the scope of application of EM bacteria is relatively wide, and it is mostly used in fields such as industry, agriculture, animal husbandry and environmental protection. Now EM bacterial liquid is a general-purpose bacterial fertilizer fermentation material that has been widely used in the agricultural liquid fertilizer market, and it is simple and easy to obtain.

CM compound bacteria can make 10 genera and 80 kinds of beneficial microorganisms in the seven categories of photosynthetic bacteria, lactic acid bacteria, yeast, bacillus, acetic acid bacteria, bifidobacteria and actinomycetes symbiosis and co-prosperity. CM composite bacteria technology is currently the most widely used bioengineering technology in the world. Compared with general biological agents, it has the advantages of complex structure, stable performance and complete functions, showing an unprecedented high-tech level.

Biogas slurry can not only completely replace chemical fertilizers, but also is the best fertilizer for producing organic food. Biogas slurry is rich in nitrogen, phosphorus, potassium, various amino acids, vitamins, proteins, gibberellins, auxins, sugars, nucleic acids, and antibiotics, as well as active resistant substances such as butyric acid, indole acetic acid, and vitamin B12 . Therefore, it has the dual functions of promoting crop growth and controlling disease occurrence.

In this example, the biogas slurry is taken from the biogas slurry discharged after anaerobic fermentation of pig farm water manure for 3 months. The fermented biogas slurry is not only tasteless, but also kills many germs during the fermentation process. Fertilizer efficiency can be increased.

Mushroom dregs are rich in crude protein, crude fat, crude fiber, ash, calcium, phosphorus, potassium, sodium, magnesium, iron, zinc, manganese, copper and other nutrients. In this embodiment, the mushroom dregs are obtained from the discarded mushroom dregs after cultivation of edible fungi as substitute materials, and the use of this kind of mushroom dregs can shorten the decomposing time.

The addition of brown sugar can promote the proliferation of biological CM and EM flora, thereby promoting plant growth, increasing stress resistance, and can also expand fruit and increase sugar content.

Selenium is an important element necessary for the human body. It has various effects such as anti-cancer and improving immunity. But at present most areas of our country are deficient in selenium, and the content of selenium in grains and vegetables in daily life is insufficient. It is necessary to develop selenium-rich foods, especially plant selenium foods that are easily absorbed and utilized by the human body. At present, there are two main ways to produce selenium-enriched vegetables. One is to grow vegetables in selenium-enriched soil. However, there are few selenium-enriched soils in my country and they are not concentrated. The production of selenium-enriched vegetables is unstable and difficult to promote on a large scale. The second way is to artificially supplement inorganic selenium such as sodium selenate. Inorganic selenium residues have certain toxicity in soil and vegetables, and their effectiveness and safety are poor. Compared with inorganic selenium, organic selenium has the advantages of less toxicity, less environmental pollution, and high utilization rate. This embodiment utilizes the complex biological flora EM, CM and organic substances provided by biogas slurry, mushroom residue, etc. to convert inorganic selenium Sodium selenate is converted into organic selenium to form organic selenium-rich liquid bacterial fertilizer.

The embodiment of the present invention also provides a preparation method of selenium-enriched liquid fertilizer, comprising:

Prepare the EM bacterial liquid, wherein, preferably, the EM bacterial liquid is obtained by mixing and culturing the EM original bacterial liquid, brown sugar water and biogas slurry according to a weight ratio of 0.4-0.6:4-6:15-25 for 20-28 hours. Brown sugar water can promote the reproduction of the flora in the EM original bacterial liquid, to help the rapid combination of the bacterial flora in the EM original bacterial liquid and the organic matter in the biogas slurry; the weight ratio of the EM original bacterial liquid to brown sugar water and biogas slurry According to the inventiveness test, the EM bacterial solution prepared within the ratio range has a stronger ability to convert inorganic selenium into organic selenium.

Then, in parts by weight, 20-25 parts of EM bacteria solution, 13-20 parts of mushroom dregs, and 1.5-2.5 parts of CM bacteria solution are added to 70-85 parts of biogas slurry, and the pH value is adjusted to 7.0- 7.5, carry out the first fermentation to obtain the first fermentation liquid; the setting of this step is conducive to further enhancing the fertilizer efficiency, and in this alkaline environment is conducive to further increasing the effectiveness, the ratio of EM bacterial liquid, mushroom residue, CM bacterial liquid, and biogas slurry According to the inventor's inventiveness test, the EM bacteria solution prepared within the range of the ratio has a stronger ability to convert inorganic selenium into organic selenium.

1-3 parts of EM bacteria liquid, 0.1-0.4 part of selenite solution are mixed with the first fermentation broth; preferably, in an embodiment of the present invention, selenate includes at least potassium selenate or sodium selenate One, the two kinds of inorganic selenium are relatively simple, potassium or sodium can be absorbed by soil or plants, and there will be no other miscellaneous elements. Adding the EM bacterial liquid and the selenate solution to the first fermentation liquid according to the above ratio can convert more inorganic selenium into organic selenium.

Then after stirring and fermenting for 4-6 days, add 0.4-0.6 part of EM former bacterial liquid, 1.8-2.2 part of CM bacterial liquid and 0.2-0.35 part of selenite solution, carry out second fermentation; Conversion of inorganic selenium. In the embodiment of the present invention, in order to achieve a better fermentation effect, preferably, the second fermentation time is preferably 15-20 days, the second fermentation includes interval stirring and exhausting, and the interval between stirring and exhausting is 1-3 days. sky.

The preparation method of the selenium-enriched liquid bacterial fertilizer provided in the embodiment of the present invention can be applied to the preparation of foliage fertilizer or root irrigation fertilizer. Specifically, preferably, the selenium-enriched liquid bacterial fertilizer can be directly used for root irrigation after being diluted 3 times, and can be used as foliar fertilizer after being diluted 5 times.

The characteristics and performance of the present invention will be described in further detail below in conjunction with the examples.

Example 1

This embodiment provides a selenium-enriched liquid bacterial fertilizer. In parts by weight, its raw materials include: 70 parts of biogas slurry, 13 parts of mushroom dregs, 21 parts of EM bacteria liquid, 0.8 parts of EM original bacteria liquid, and 3.3 parts of CM bacteria , 0.3 parts of sodium selenite, and 4 parts of brown sugar.

This example provides a method for preparing selenium-enriched liquid bacterial fertilizer, comprising: dissolving 4 parts of brown sugar in water to prepare brown sugar water, mixing 0.4 parts of EM original bacterial liquid with 4 parts of brown sugar water and 15 parts of biogas slurry for 20 -28 hours to get the EM bacterial liquid.

Add 20 parts of EM bacterial liquid, 13 parts of mushroom dregs, and 1.5 parts of CM bacterial liquid to 70 parts of biogas slurry, adjust the pH value to 7.0, and carry out the first fermentation to obtain the first fermentation liquid; add 1 part of EM bacterial liquid again , 0.1 part of sodium selenite solution; carry out stirring fermentation; after 4-6 days, add 0.4 part of EM original bacterial solution, 1.8 parts of CM bacterial solution and 0.2 part of sodium selenite solution for the second fermentation, and the fermentation time is 15- 20 days, the second fermentation process includes stirring and exhausting at intervals, the interval between stirring and exhausting is 1-3 days, and the selenium-enriched liquid bacterial fertilizer is obtained.

Dilute the selenium-enriched liquid bacterial fertilizer provided in this example by 5 times to prepare selenium-enriched foliage fertilizer.

Example 2

This embodiment provides a kind of selenium-enriched liquid bacterial fertilizer. The raw materials include: 85 parts of biogas slurry, 20 parts of mushroom dregs, 28 parts of EM bacterial liquid, 1.2 parts of EM original bacterial liquid, and 4.7 parts of CM bacteria in parts by weight. , 0.75 parts of sodium selenite, and 6 parts of brown sugar.

This example provides a preparation method of selenium-enriched liquid bacterial fertilizer, comprising: dissolving 6 parts of brown sugar in water to prepare brown sugar water, mixing 0.6 parts of EM original bacterial liquid with 6 parts of brown sugar water and 25 parts of biogas slurry for 20 -28 hours to get the EM bacterial liquid.

Add 25 parts of EM bacterial liquid, 20 parts of mushroom dregs, and 2.5 parts of CM bacterial liquid to 85 parts of biogas slurry, adjust the pH value to 7.0, and carry out the first fermentation; add 3 parts of EM bacterial liquid and 0.4 part of selenous acid Sodium solution; carry out stirring fermentation; after 4-6 days, add 0.6 parts of EM original bacterial liquid, 2.2 parts of CM bacterial liquid and 0.35 parts of sodium selenite solution for the second fermentation, the fermentation time is 15-20 days, the second fermentation The process includes stirring and degassing at intervals, the interval between stirring and degassing is 1-3 days, and the selenium-enriched liquid bacterial fertilizer is obtained.

Dilute the selenium-enriched liquid bacterial fertilizer provided in this example by 5 times to prepare selenium-enriched foliage fertilizer.

Example 3

This embodiment provides a selenium-enriched liquid bacterial fertilizer. In parts by weight, its raw materials include: 72 parts of biogas slurry, 15 parts of mushroom dregs, 23 parts of EM bacterial liquid, 0.9 part of EM original bacterial liquid, and 3.5 parts of CM bacteria , 0.35 parts of sodium selenite, and 4.5 parts of brown sugar.

This embodiment provides a preparation method of selenium-enriched liquid bacterial fertilizer, comprising: dissolving 4.5 parts of brown sugar in water to prepare brown sugar water, mixing 0.4 part of EM original bacterial liquid with 4.5 parts of brown sugar water and 20 parts of biogas slurry for 20 -28 hours to get the EM bacterial liquid.

Add 21 parts of EM bacterial liquid, 15 parts of mushroom residue, and 1.6 parts of CM bacterial liquid to 72 parts of biogas slurry, adjust the pH value to 7.0, and carry out the first fermentation; add 2 parts of EM bacterial liquid and 0.15 part of selenous acid Sodium solution; carry out stirring fermentation; after 4-6 days, add 0.5 parts of EM original bacterial solution, 1.9 parts of CM bacterial solution and 0.3 part of sodium selenite solution for the second fermentation, the fermentation time is 15-20 days, the second fermentation The process includes stirring and degassing at intervals, the interval between stirring and degassing is 1-3 days, and the selenium-enriched liquid bacterial fertilizer is obtained.

Dilute the selenium-enriched liquid bacterial fertilizer provided in this example by 5 times to prepare selenium-enriched foliage fertilizer.

Example 4

This embodiment provides a kind of selenium-enriched liquid bacterial fertilizer. The raw materials include: 80 parts of biogas slurry, 18 parts of mushroom dregs, 27 parts of EM bacteria liquid, 1.1 parts of EM original bacteria liquid, and 4.5 parts of CM bacteria in parts by weight. , 0.6 parts of sodium selenite, and 5.5 parts of brown sugar.

This embodiment provides a preparation method of selenium-enriched liquid bacterial fertilizer, comprising: dissolving 5.5 parts of brown sugar in water to prepare brown sugar water, mixing 0.5 part of EM original bacterial liquid with 5.5 parts of brown sugar water and 22 parts of biogas slurry for 20 -28 hours to get the EM bacterial liquid.

Add 24 parts of EM bacterial liquid, 18 parts of mushroom residue, and 2.3 parts of CM bacterial liquid to 80 parts of biogas slurry, adjust the pH value to 7.0, and carry out the first fermentation; add 3 parts of EM bacterial liquid and 0.4 part of selenous acid Sodium solution; carry out stirring fermentation; after 4-6 days, add 0.6 parts of EM original bacterial solution, 2.2 parts of CM bacterial solution and 0.2 part of sodium selenite solution for the second fermentation, the fermentation time is 15-20 days, the second fermentation The process includes stirring and degassing at intervals, the interval between stirring and degassing is 1-3 days, and the selenium-enriched liquid bacterial fertilizer is obtained.

Dilute the selenium-enriched liquid bacterial fertilizer provided in this example by 5 times to prepare selenium-enriched foliage fertilizer.

Example 5

This embodiment provides a kind of selenium-enriched liquid bacterial fertilizer. The raw materials include: 74 parts of biogas slurry, 16 parts of mushroom dregs, 24 parts of EM bacteria liquid, 1 part of EM original bacteria liquid, and 3.7 parts of CM bacteria in parts by weight. , 0.4 parts of sodium selenite, and 5 parts of brown sugar.

This example provides a method for preparing selenium-enriched liquid bacterial fertilizer, comprising: dissolving 5.5 parts of brown sugar in water to prepare brown sugar water, mixing 0.5 part of EM original bacterial liquid with 5 parts of brown sugar water and 18 parts of biogas slurry for 20 -28 hours to get the EM bacterial liquid.

Add 22 parts of EM bacterial liquid, 16 parts of mushroom residue, and 1.5 parts of CM bacterial liquid to 74 parts of biogas slurry, adjust the pH value to 7.0, and carry out the first fermentation; add 2 parts of EM bacterial liquid and 0.2 part of selenous acid Sodium solution; carry out stirring fermentation; after 4-6 days, add 0.5 parts of EM original bacterial solution, 2.2 parts of CM bacterial solution and 0.2 part of sodium selenite solution for the second fermentation, the fermentation time is 15-20 days, the second fermentation The process includes stirring and degassing at intervals, the interval between stirring and degassing is 1-3 days, and the selenium-enriched liquid bacterial fertilizer is obtained.

Dilute the selenium-enriched liquid bacterial fertilizer provided in this example by 5 times to prepare selenium-enriched foliage fertilizer.

Example 6

This embodiment provides a kind of selenium-enriched liquid bacterial fertilizer. The raw materials include: 77 parts of biogas slurry, 17 parts of mushroom dregs, 26 parts of EM bacteria liquid, 1.05 parts of EM original bacteria liquid, and 4.1 parts of CM bacteria in parts by weight. , 0.5 parts of sodium selenite, and 5.2 parts of brown sugar.

This embodiment provides a preparation method of selenium-enriched liquid bacterial fertilizer, comprising: dissolving 5.2 parts of brown sugar in water to prepare brown sugar water, mixing 0.45 parts of EM original bacterial liquid with 5.2 parts of brown sugar water and 19 parts of biogas slurry for 20 -28 hours to get the EM bacterial liquid.

Add 24 parts of EM bacterial liquid, 17 parts of mushroom residue, and 2.3 parts of CM bacterial liquid to 77 parts of biogas slurry, adjust the pH value to 7.0, and carry out the first fermentation; add 2 parts of EM bacterial liquid and 0.3 part of selenous acid Sodium solution; carry out stirring fermentation; after 4-6 days, add 0.6 parts of EM original bacterial solution, 1.9 parts of CM bacterial solution and 0.2 part of sodium selenite solution for the second fermentation, the fermentation time is 15-20 days, the second fermentation The process includes stirring and degassing at intervals, the interval between stirring and degassing is 1-3 days, and the selenium-enriched liquid bacterial fertilizer is obtained.

Dilute the selenium-enriched liquid bacterial fertilizer provided in this example by 5 times to prepare selenium-enriched foliage fertilizer.

Example 7

This embodiment provides a kind of selenium-enriched liquid bacterial fertilizer. The raw materials include: 77 parts of biogas slurry, 17 parts of mushroom dregs, 26 parts of EM bacteria liquid, 1.05 parts of EM original bacteria liquid, and 4.1 parts of CM bacteria in parts by weight. , 0.5 parts of sodium selenite, and 5.2 parts of brown sugar.

This embodiment provides a preparation method of selenium-enriched liquid bacterial fertilizer, comprising: dissolving 5.2 parts of brown sugar in water to prepare brown sugar water, mixing 0.45 parts of EM original bacterial liquid with 5.2 parts of brown sugar water and 19 parts of biogas slurry for 20 -28 hours to get the EM bacterial liquid.

Add 24 parts of EM bacterial liquid, 17 parts of mushroom residue, and 2.3 parts of CM bacterial liquid to 77 parts of biogas slurry, adjust the pH value to 7.5, and carry out the first fermentation; add 2 parts of EM bacterial liquid and 0.3 part of selenous acid Sodium solution; carry out stirring fermentation; after 4-6 days, add 0.6 parts of EM original bacterial solution, 1.9 parts of CM bacterial solution and 0.2 part of sodium selenite solution for the second fermentation, the fermentation time is 15-20 days, the second fermentation The process includes stirring and degassing at intervals, the interval between stirring and degassing is 1-3 days, and the selenium-enriched liquid bacterial fertilizer is obtained.

Dilute the selenium-enriched liquid bacterial fertilizer provided in this example by 5 times to prepare selenium-enriched foliage fertilizer.

Example 8

This embodiment provides a kind of selenium-enriched liquid bacterial fertilizer. The raw materials include: 77 parts of biogas slurry, 17 parts of mushroom dregs, 26 parts of EM bacteria liquid, 1.05 parts of EM original bacteria liquid, and 4.1 parts of CM bacteria in parts by weight. , 0.5 parts of sodium selenite, and 5.2 parts of brown sugar.

This embodiment provides a preparation method of selenium-enriched liquid bacterial fertilizer, comprising: dissolving 5.2 parts of brown sugar in water to prepare brown sugar water, mixing 0.45 parts of EM original bacterial liquid with 5.2 parts of brown sugar water and 19 parts of biogas slurry for 20 -28 hours to get the EM bacterial liquid.

Add 24 parts of EM bacterial liquid, 17 parts of mushroom residue, and 2.3 parts of CM bacterial liquid to 77 parts of biogas slurry, adjust the pH value to 7.3, and carry out the first fermentation; add 2 parts of EM bacterial liquid and 0.3 part of selenous acid Sodium solution; carry out stirring fermentation; after 4-6 days, add 0.6 parts of EM original bacterial solution, 1.9 parts of CM bacterial solution and 0.2 part of sodium selenite solution for the second fermentation, the fermentation time is 15-20 days, the second fermentation The process includes stirring and degassing at intervals, the interval between stirring and degassing is 1-3 days, and the selenium-enriched liquid bacterial fertilizer is obtained.

Dilute the selenium-enriched liquid bacterial fertilizer provided in this example by 5 times to prepare selenium-enriched foliage fertilizer.

Comparative example 1

This embodiment provides a kind of selenium-enriched liquid bacterial fertilizer. The raw materials include: 60 parts of biogas slurry, 30 parts of mushroom dregs, 20 parts of EM bacteria liquid, 2 parts of EM original bacteria liquid, and 5 parts of CM bacteria in parts by weight. , 0.2 parts of sodium selenite, and 3 parts of brown sugar.

This embodiment provides a preparation method of selenium-enriched liquid bacterial fertilizer, comprising: dissolving 3 parts of brown sugar in water to prepare brown sugar water, mixing 1 part of EM original bacterial solution with 3 parts of brown sugar water and 12 parts of biogas slurry for 20 -28 hours to get the EM bacterial liquid.

Add 15 parts of EM bacterial liquid, 30 parts of mushroom dregs, and 3 parts of CM bacterial liquid to 60 parts of biogas slurry, adjust the pH value to 7.0, carry out the first fermentation, and obtain the first fermentation liquid; add 5 parts of EM bacterial liquid again , 0.1 part of sodium selenite solution; carry out stirring fermentation; after 4-6 days, add 1 part of EM original bacterial solution, 2 parts of CM bacterial solution and 0.1 part of sodium selenite solution for the second fermentation, the fermentation time is 15-20 The second fermentation process includes stirring and exhausting at intervals, and the interval between stirring and exhausting is 1-3 days to obtain selenium-enriched liquid bacterial fertilizer.

Dilute the selenium-enriched liquid bacterial fertilizer provided in this example by 5 times to prepare selenium-enriched foliage fertilizer.

Experimental example 1

The selenium-enriched foliar fertilizer prepared in Examples 1-8 and Comparative Example 1 is set as experimental group 1-9, clear water is set as experimental group 10 as a control group, and 60 mg/kg of sodium selenite is set as experimental group 11 ;

Take 1100 unharvested pakchoi or lettuce with the same maturity, 100 plants as a group, set up as experimental group 1-11, spray on the leaves of pakchoi or lettuce in experimental group 1-9 15 days before harvesting The selenium-enriched foliar fertilizer provided by Examples 1-8 and Comparative Example 1 sprays an equal amount of clear water to the pakchoi or lettuce leaves of the experimental group 10, and sprays an equal amount of water to the pakchoi or lettuce of the experimental group 11. 60mg/kg of sodium selenite.

The yield, quality, organic selenium, and inorganic selenium content of experimental groups 1-11 are compared as follows:

Table 1 Effects of different treatments on the yield, quality and selenium content of pakchoi

Table 2 Effects of different treatments on the yield, quality and selenium content of lettuce

From Table 1 in conjunction with Table 2, it can be seen that the yield and Vc content of pakchoi or lettuce in experimental group 11, total selenium and organic selenium content are higher than experimental group 10, the yield and Vc content of pakchoi or lettuce in experimental group 1-9, The content of total selenium and organic selenium is much higher than that of the experimental group 11. This result shows that the foliar fertilizer prepared by the selenium-enriched liquid bacterial fertilizer provided by the present invention can increase crop yield and crop quality more than inorganic selenium fertilizer.

The output, Vc content, total selenium and organic selenium content of the pakchoi or lettuce of the experimental group 1-6 were higher than the experimental group 9, and the result data of the experimental group 6 was the best, and the raw materials of the experimental group 9 and the experimental group 1-6 The same, the same production steps, indicating that the raw material ratio of selenium-enriched liquid bacterial fertilizer has a greater impact on the prepared foliar fertilizer, and the foliar fertilizer prepared within the ratio provided by the embodiments of the present invention is compared to the inorganic selenium fertilizer. , can increase crop yield and improve crop quality.

The yield, Vc content, total selenium and organic selenium content of the pakchoi or lettuce of experimental group 6-8 are lower than experimental group and experimental group 8 is the best, experimental group 8 and experimental group 6-7 have the same raw materials and proportioning, The production steps are the same, indicating that the pH range of the first fermentation has a greater impact on the prepared foliar fertilizer, and the foliar fertilizer prepared within the proportioning range provided by the embodiments of the present invention is more able to improve the selenium fertilizer. Crop yield, improve crop quality.

In summary, the selenium-enriched liquid bacterial fertilizer provided by the present invention utilizes waste resources such as biogas slurry and mushroom dregs for recycling, reduces production costs, and slows down environmental pollution. It can provide vegetables with various nutrients and promote growth. The disadvantages of high fertilizer usage, high time efficiency, and low toxicity improve the added value of vegetable products; the preparation method of the selenium-enriched liquid bacterial fertilizer provided by the invention has simple technology and can effectively prepare selenium with excellent performance. Liquid fertilizer.

The embodiments described above are some, not all, embodiments of the present invention. The detailed description of the embodiments of the invention is not intended to limit the scope of the claimed invention but to represent only selected embodiments of the invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

Claims (10)

1. A selenium-enriched liquid bacterial fertilizer is characterized in that, in parts by weight, its raw materials include: 70-85 parts of biogas slurry, 13-20 parts of mushroom dregs, 21-28 parts of EM bacterial liquid, and EM original bacterial liquid 0.8-1.2 parts, CM bacteria 3.3-4.7 parts, selenite 0.3-0.75 parts, and brown sugar 4-6 parts. 2. The selenium-enriched liquid bacterial fertilizer according to claim 1 is characterized in that, in parts by weight, the biogas slurry is 72-80 parts, the mushroom dregs is 15-18 parts, and the EM bacterial liquid is 23-80 parts. 27 parts, 0.9-1.1 parts of EM original bacteria solution, 3.5-4.5 parts of the CM bacteria, 0.35-0.6 parts of the sodium selenite, and 4.5-5.5 parts of the brown sugar. 3. The selenium-enriched liquid bacterial fertilizer according to claim 2 is characterized in that, in parts by weight, the biogas slurry is 74-77 parts, the mushroom dregs is 16-17 parts, and the EM bacterial liquid is 24-77 parts. 26 parts, 1-1.05 parts of EM original bacteria liquid, 3.7-4.1 parts of the CM bacteria, 0.4-0.5 parts of the selenite, and 5-5.2 parts of the brown sugar. 4. The selenium-enriched liquid bacterial fertilizer according to claim 1, characterized in that, the biogas slurry is the biogas slurry discharged after 3 months of anaerobic fermentation of pig farm manure. 5. The selenium-enriched liquid bacterial fertilizer according to claim 1, characterized in that, the mushroom dregs are discarded mushroom dregs after cultivation of edible fungi as substitute materials. 6. a preparation method of selenium-enriched liquid bacterial fertilizer, is characterized in that, comprising: In parts by weight, add 20-25 parts of EM bacteria solution, 13-20 parts of mushroom dregs, and 1.5-2.5 parts of CM bacteria solution to 70-85 parts of biogas slurry, and adjust the pH value to 7.0-7.5 , carrying out the first fermentation to obtain the first fermentation liquid; Mixing 1-3 parts of EM bacteria solution and 0.1-0.4 part of selenite solution with the first fermentation liquid; stirring and fermenting; After 4-6 days, add 0.4-0.6 parts of EM original bacterial liquid, 1.8-2.2 parts of CM bacterial liquid and 0.2-0.35 parts of selenite solution for second fermentation; obtain the selenium-enriched liquid bacterial fertilizer. 7. The preparation method of the selenium-enriched liquid bacterial fertilizer according to claim 6, characterized in that, in parts by weight, the EM bacterial liquid is composed of 0.4-0.6 parts of the EM original bacterial liquid and 4-6 parts of the Brown sugar and 15-25 parts of the biogas slurry are mixed and cultivated for 20-28 hours. 8. the preparation method of selenium-enriched liquid bacterial fertilizer according to claim 6 is characterized in that, the time of described second fermentation is 15-20 days, and described second fermentation comprises interval stirring and exhausting, so The interval between stirring and exhausting is 1-3 days. 9. The preparation method of selenium-enriched liquid bacterial fertilizer according to claim 6, wherein the selenite comprises at least one of potassium selenite or sodium selenite. 10. the preparation method of selenium-enriched liquid bacterial fertilizer as claimed in claim 6 is in the application in the preparation foliage fertilizer or irrigation root fertilizer.