CN107041341A - A kind of Artificial Insemination For Honey Bees syringe needle drawing method and its drawing device - Google Patents

Abstract

The invention provides a bee artificial insemination needle drawing method and a drawing device thereof, and relates to the technical field of bee breeding. The honeybee artificial insemination needle drawing device includes a low-voltage power supply, a sleeve type heating module and a needle fixing sleeve; the sleeve The heating module includes a linear through-hole arranged along its axis; the tube body for processing the needle is inserted into the through-hole; the sleeve-type heating module is connected to the low-voltage power supply for The inner tube body is heated; the needle fixing sleeve is at least connected to one end of the tube body for stretching the tube body and making the tube body thinner. The invention is simple in structure and easy to use. The casing type heating module can heat the pipe body evenly. When the material of the pipe body is melted and can be plastically deformed, the needle fixing sleeve is used to stretch the pipe body to make the pipe body thinner until it meets the requirements of bees. Specification requirements for artificial insemination needles.

Description

A kind of bee artificial insemination needle drawing method and its drawing device

technical field

The invention relates to the technical field of bee breeding, in particular to a method for drawing a honeybee artificial insemination needle and a drawing device thereof.

Background technique

The existing honeybee artificial insemination equipment consists of an insemination instrument, a stereo microscope (and a lighting system), a carbon dioxide anesthesia system, and the like. The needle is an important accessory device in the process of artificial insemination of bees.

When honeybees are artificially inseminated, first select a pure, large, robust, lively and sexually mature drone, and use artificial methods to force the drone to ejaculate. Then use the needle to collect semen. When collecting semen, first inhale a little physiological fluid and air bubbles through the needle, and then make the needle contact with the semen. With the help of the viscosity of the semen itself, the semen is completely sucked into the needle; After the drone semen is needed, the needle is closed with physiological fluid and ready to inseminate the queen bee.

After the queen bee is anesthetized, open the stinging chamber of the queen bee, adjust the angle between the queen bee and the needle, and remove the physiological fluid and air bubbles in the needle tip. With the cooperation of the probe, carefully insert the needle tip into the oviduct of the queen bee, and slowly inject the semen Inject into the queen bee body. The queen bee injects 8 microliters of semen for one-time artificial insemination, and injects 4 microliters to 5 microliters of semen for each of the two inseminations. The difficulty of queen bee artificial insemination technology is to successfully insert the insemination needle into the appropriate position of the queen bee's oviduct. Therefore, the quality of the insemination needle plays an important role in the success rate of queen bee artificial insemination.

However, the present needles for artificial insemination of bees are made entirely by manual techniques, the inner and outer diameters of the needles cannot be accurately controlled, the drawing is difficult, the finished products are of different specifications, the yield is low, the quality of the needles for insemination is poor, and the success rate of insemination of the queen bee is low.

Contents of the invention

The purpose of the present invention is to provide a needle drawing method to solve the problem in the prior art that the production of needles for artificial insemination of bees depends entirely on manual skills, the inner and outer diameters of the needles cannot be accurately controlled, the drawing is difficult, and the finished products have different specifications. , The yield rate is low, the insemination needle quality is poor, and the technical problems of the queen bee insemination success rate are low.

Another object of the present invention is to provide a bee artificial insemination needle puller to solve the problem in the prior art that the production of bee artificial insemination needles is entirely manual, the inner and outer diameters of the needle cannot be accurately controlled, and the drawing is difficult. The specifications of finished products are different, the yield of finished products is low, the quality of insemination needles is poor, and the technical problems of low success rate of queen bee insemination.

In order to solve the above technical problems, the present invention provides a bee artificial insemination needle puller, which includes: a low-voltage power supply, a sleeve type heating module and a needle fixing sleeve;

The sleeve-type heating module includes a linear through-hole arranged along its axis;

A tube body for processing needles is inserted into the through hole;

The sleeve-type heating module is connected to the low-voltage power supply, and is used to heat the pipe body in the through hole;

The needle fixing sleeve is at least connected to one end of the tubular body, and is used for stretching the tubular body and making the tubular body thinner.

The present invention is simple in structure and easy to use. The casing type heating module can heat the tube body uniformly. When the material of the tube body is melted and can be plastically deformed, one end of the tube body is fixed by a needle fixing sleeve, and the other end of the tube body is pulled out. , so that the tube body becomes thinner until it meets the specification requirements of a needle for artificial insemination of bees.

In the prior art, the tube body is baked on an alcohol lamp, and the temperature at which the tube body is heated is difficult to control, and in the axial direction and the circumferential direction of the tube body, various parts of the tube body are heated to different degrees. When stretching, the degree of stretching and deformation of each part is also different, resulting in uneven diameter and thickness of the needle; it is also difficult to unify the specifications among different products; and the yield is low, the quality of the insemination needle is poor, and the success rate of queen bee insemination is low.

However, the present invention can effectively control the temperature of the through hole in the sleeve type heating module through a low-voltage power supply, and then control the heating time of the tube body to effectively ensure that different batches, different tubes in the same batch, and different parts of the tube are uniform. A uniform heat treatment is obtained, and under the action of a uniform tensile external force, the high yield of needles can be effectively guaranteed, and the aperture and outer diameter of the processed needles are uniform and fixed.

Further, the tube body is a capillary glass tube or a capillary plastic tube.

For example, the tube body is a polyethylene capillary plastic tube.

Further, the output voltage of the low-voltage power supply is adjustable.

Further, the low-voltage power supply is an adjustable low-voltage power supply of 1-5V.

More preferably, the low-voltage power supply is a 2V adjustable low-voltage power supply.

Further, the sleeve-type heating module is a helically wound resistance wire or a tubular ceramic heating sheet;

Alternatively, the sleeve-type heating module is a heating wire mesh wound into a tubular body.

Further, the honeybee artificial insemination needle puller also includes a pulling device for stretching the tube body; the pulling device is arranged opposite to the needle fixing sleeve, and the two are respectively connected to the tube body. Both ends are connected.

Further, the pulling device is a hydraulic cylinder, an air cylinder or an electric telescopic rod or the like.

Further, the pulling device is a pull-down weight;

The sleeve-type heating module is arranged vertically, the needle fixing sleeve is arranged on the upper end of the sleeve-type heating module and connected with the upper end of the tube body; the pull-down weight is arranged on the sleeve-type heating module The lower end is connected with the lower end of the pipe body.

Further, the honeybee artificial insemination needle puller also includes a linear guide track and a slider, the linear guide track is arranged parallel to the sleeve type heating module; the needle fixing sleeve is relatively fixed to the linear guide track Setting, the slider is slidably arranged on the linear guide rail; the slider is arranged opposite to the needle fixing sleeve, the needle fixing sleeve is connected with one end of the tube body, and the slider is connected with the other end of the tube body .

Further, the pulling device is connected with the slider, and pulls the tubular body through the slider.

The needle fixing sleeve is various types of tube body fixing sleeves or tube body fixing devices.

When the drawing device is used, the tube body is preferably made of a capillary glass tube, and when a capillary plastic tube is used, the two ends of the capillary plastic tube can also be pulled manually.

The needles made of glass and plastic prepared by the bee artificial insemination needle puller of the present invention have uniform thickness, high yield and uniform specifications, effectively ensuring the success rate of bee artificial insemination.

The present invention also discloses a needle drawing method using the above-mentioned bee artificial insemination needle drawing device, which comprises the following steps:

Step 1, passing one end of the tube body through the through hole of the sleeve type heating module, and fixing it on the needle fixing sleeve;

Step 2, turn on the low-voltage power supply, and use the sleeve type heating module to heat the pipe body to melt the pipe body;

Step 3, stretching the tube body evenly and forcefully to make the tube body thinner to the set diameter, and make a needle blank.

Further, the front end of the needle blank is made into a needle point shape.

Further, the rear end of the needle blank is made into a bell-mouth shape.

For capillary plastic tube material, use a cutter to cut the needle blank from the tube body, or, when removing the excess tube body at both ends of the needle blank, keep the part connected to the rear end of the needle blank to be thicker than the tube body, and the rear end of the needle blank is natural Form a bell-mouth shape, and then heat the bell-mouth end until smooth, ready to be inserted into the injector.

Further, the needle drawing method also includes a grinding and deburring process.

Further, in the first step, the sleeve-type heating module is arranged vertically, the upper end of the tube body is fixedly set by the needle fixing sleeve, and the lower end of the tube body hangs a pull-down weight.

Further, when the tube body is a glass tube, the weight of the pull-down weight is 180-200g, and the heating temperature of the sleeve-type heating module is 800°C;

When the pipe body is a plastic pipe, the weight of the pull-down weight is 35-40g, and the heating temperature of the sleeve-type heating module is 150°C.

Adopt above-mentioned technical scheme, the present invention has following beneficial effect:

The needle drawing method of the present invention has the advantages of simple operation, more uniform heating of the pipe material, higher needle drawing efficiency, lower production cost, higher yield of needles produced, and 12.6% increase in the success rate of queen bee insemination, which fills the gap in this field. blank.

Description of drawings

In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the accompanying drawings that need to be used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the accompanying drawings in the following description These are some implementations of the present invention. For those skilled in the art, other drawings can also be obtained according to these drawings without creative work.

Fig. 1 is the schematic structural view of the honeybee artificial insemination needle puller provided by Embodiment 1 of the present invention;

Fig. 2 is the schematic structural view of the bee artificial insemination needle puller provided by Embodiment 2 of the present invention;

Fig. 3 is a flow chart of the needle drawing method provided by Embodiment 3 of the present invention;

Fig. 4 is a schematic diagram of the structure of the finished needle made of plastic material in Example 3 of the present invention;

Fig. 5 is a schematic diagram of the structure of a finished needle made of glass material in Example 3 of the present invention;

Fig. 6 is a schematic structural view of the needle fixing sleeve in Embodiment 1 of the present invention.

Reference signs:

1-tube body; 2-needle; 2a-tail; 2b-plastic tube; 10-low voltage power supply; 20-tube heating module; 21-through hole; 30-needle fixing sleeve; 31-inner tube; ; 33-retractable end; 40-drawing device; 50-linear guide rail; 51-slider.

detailed description

The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Apparently, the described embodiments are part of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" etc. The indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, or in a specific orientation. construction and operation, therefore, should not be construed as limiting the invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and should not be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that unless otherwise specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection. Connected, or integrally connected; it may be mechanically connected or electrically connected; it may be directly connected or indirectly connected through an intermediary, and it may be the internal communication of two components. Those of ordinary skill in the art can understand the specific meanings of the above terms in the present invention in specific situations.

The present invention will be further explained below in combination with specific embodiments.

Example 1

As shown in FIG. 1 , a bee artificial insemination needle puller provided in this embodiment includes: a low-voltage power supply 10 , a sleeve type heating module 20 and a needle fixing sleeve 30 ;

The casing heating module 20 includes a linear through hole 21 arranged along its axis;

The tube body 1 for processing needles is inserted into the through hole 21;

The casing heating module 20 is connected to the low-voltage power supply 10 for heating the pipe body 1 in the through hole 21;

The needle fixing sleeve 30 is connected with one end of the tube body 1, stretches the other end of the tube body 1 and makes the tube body 1 thinner.

The present invention is simple in structure and easy to use. The sleeve type heating module 20 can heat the tube body 1 evenly. The other end of the body makes the tube body thinner until it meets the specification requirements of the needle for artificial insemination of bees.

In the prior art, the tube body 1 is baked on an alcohol lamp, and the temperature at which the tube body 1 is heated is difficult to control, and in the axial direction and the circumferential direction of the tube body 1, various parts of the tube body 1 are heated to different degrees. Therefore, when the external force is stretched, the degree of stretching and deformation of each part is also different, resulting in uneven diameter and thickness of the needle; it is also difficult to unify the specifications among different products; and the yield is low, the quality of the insemination needle is poor, and the queen bee is successfully inseminated low rate.

However, the present invention can effectively control the temperature of the through hole 21 in the casing heating module 20 through the low-voltage power supply 10, and then control the heating time of the tube body 1 to effectively ensure that different batches and different tube bodies 1 and tubes in the same batch Different parts of the body 1 are uniformly heat-treated, and under the action of a unified external tensile force, a high yield of needles can be effectively ensured, and the processed needles have a uniform aperture and outer diameter and remain fixed.

The tube body 1 is a capillary glass tube; the output voltage of the low-voltage power supply 10 is adjustable.

Preferably, the low voltage power supply 10 is a 2V adjustable low voltage power supply 10 .

In this embodiment, the sleeve-type heating module 20 is a helically wound resistance wire, of course, it can also be other tube-shaped heating devices.

The honeybee artificial insemination needle puller also includes a puller 40 for stretching the tube body 1; the puller 40 is arranged opposite to the needle holder 30, and the two are respectively connected to the two ends of the tube body 1.

In this embodiment, the pulling device 40 is a pull-down weight. Of course, the pulling device 40 can also be a hydraulic cylinder, an air cylinder, or an electric telescopic rod with adjustable and controllable pulling force.

The sleeve-type heating module 20 is vertically arranged, and the needle fixing sleeve 30 is arranged on the upper end of the sleeve-type heating module 20 and connected with the upper end of the tube body 1; the lower end connection.

As shown in Figure 6, the needle holder 30 includes an inner tube 31 and an outer sleeve 32. The inner tube 31 is provided with a retractable end 33, and the retractable end 33 of the inner tube 31 and the outer sleeve 32 are respectively provided with an external tapered thread and an internal tapered thread. screw thread, the retractable end 33 of the inner tube 31 is an expandable and shrinkable structure, that is, the retractable end 33 of the inner tube 31 is provided with strip-shaped seams along the axial direction, and during the process of tightening the outer sleeve 32, the outer sleeve 32 squeezes the inner tube 31 to shrink The end 33 is tightened radially, and then clamps the pipe inserted in the middle.

The needle fixing sleeve can also be a variety of tube body fixing devices that allow the tube body to pass through in one direction and reverse lock, such as the tapered rubber inner sleeve and outer sleeve that cooperate with each other, and the tube body can pass through the middle of the inner sleeve smoothly Through the hole, when the pipe body is pulled in the opposite direction, the inner sleeve and the outer sleeve pass through the tapered surfaces that cooperate with each other, forcing the inner sleeve to tighten and then lock the pipe body. The needles prepared by the needle drawing device of the present invention have uniform thickness, high yield and uniform specifications, effectively ensuring the success rate of bee artificial insemination.

Example 2

The structure of this embodiment is basically the same as that of Embodiment 1, the difference is that:

The tube body 1 is a polyethylene capillary plastic tube.

In addition, as shown in Figure 2, the bee artificial insemination needle puller also includes a linear guide rail 50 and a slider 51, the linear guide rail 50 is arranged in parallel with the sleeve type heating module 20; the needle fixing sleeve 30 is opposite to the linear guide rail 50 Fixed setting, the slider 51 is slidably arranged on the linear guide rail 50; the slider 51 is arranged opposite to the needle fixing sleeve 30, the needle fixing sleeve 30 is connected to one end of the tube body 1, and the slider 51 is connected to the other end of the tube body 1 connect.

The drawing device 40 is connected with the slider 51 and stretches the pipe body 1 through the slider 51 .

Certainly, when output is less, also can adopt the two ends of manpower drawing capillary plastic tube.

Example 3

As shown in Figure 3, the present embodiment discloses a needle drawing method using the above-mentioned honeybee artificial insemination needle drawing device, which comprises the following steps:

Step 1, passing one end of the tube body 1 through the through hole 21 of the sleeve type heating module 20, and fixing it on the needle fixing sleeve 30;

Step 2, turn on the low-voltage power supply 10, and use the sleeve type heating module 20 to heat the pipe body 1 to melt the pipe body 1;

Step 3: Stretch the tube body 1 evenly and forcefully to make the tube body 1 thinner to the set diameter, and make a needle blank.

Cut off the front end of the needle blank to make a needle point.

The rear end of the needle blank is made into a bell-mouth shape.

For the material of capillary plastic tube, cut the needle blank from the tube body 1 with a knife, or remove the excess tube body 1 at both ends of the needle blank, keep the thicker tube body 1 connected to the rear end of the needle blank, and keep the thicker tube body 1 at the rear end of the needle blank. Naturally form a flared shape, then heat the flared end until smooth. As shown in FIG. 4 , the tail 2 a of the finished needle 2 made of plastic material is set in a bell-shaped shape, which is convenient for inserting into the injector.

When the tube body 1 is a capillary glass tube, as shown in FIG. 5 , the tail end of the finished needle 2 made of glass material can be connected with a trumpet-shaped plastic tube 2b for easy insertion into the sample injector.

The needle drawing method of the present invention has the advantages of simple operation, more uniform heating of the pipe material, higher needle drawing efficiency, lower production cost, higher yield of needles produced, and 12.6% increase in the success rate of queen bee insemination, which fills the gap in this field. blank.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, rather than limiting them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: It is still possible to modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technical solutions of the various embodiments of the present invention. scope.

Claims (10)

1. a kind of Artificial Insemination For Honey Bees syringe needle drawing device, it is characterised in that it includes：Low-tension supply, bushing type heating module with And syringe needle fixed cover；

Described sleeve pipe formula heating module includes the linear through hole set along its axis；

Body for processing needle head is inserted into the through hole；

Described sleeve pipe formula heating module is connected with the low-tension supply, for being heated to the body in through hole；

The one end of the syringe needle fixed cover at least with the body is connected, for stretching the body and body is attenuated.

2. Artificial Insemination For Honey Bees syringe needle drawing device according to claim 1, it is characterised in that the body is capillary glass Pipe or capillary plastic tube.

3. Artificial Insemination For Honey Bees syringe needle drawing device according to claim 1, it is characterised in that the low-tension supply is 1~ 5V can adjust low-tension supply.

4. Artificial Insemination For Honey Bees syringe needle drawing device according to claim 1, it is characterised in that described sleeve pipe formula heating module The ceramic heating plate of resistance wire or tubulose for spiral winding；

Or, described sleeve pipe formula heating module is the heating silk screen for being wound into pipe body shape.

5. Artificial Insemination For Honey Bees syringe needle drawing device according to claim 1, it is characterised in that the syringe needle drawing device is also wrapped Include the draw-off gear for stretching the body；The draw-off gear is oppositely arranged with the syringe needle fixed cover, both respectively with The two ends connection of the body.

6. Artificial Insemination For Honey Bees syringe needle drawing device according to claim 5, it is characterised in that the draw-off gear is hydraulic pressure Cylinder, cylinder or electric expansion bar；

Or, the draw-off gear is drop-down weight；Described sleeve pipe formula heating module is vertically arranged, and the syringe needle fixed cover is set It is connected in the upper end of described sleeve pipe formula heating module with the upper end of the body；The drop-down weight is arranged on described sleeve pipe formula and added The lower end of thermal modules, is connected with the lower end of the body.

7. Artificial Insemination For Honey Bees syringe needle drawing device according to claim 6, it is characterised in that the syringe needle drawing device is also wrapped Straight-line guidance track and sliding block are included, the straight-line guidance track be arranged in parallel with described sleeve pipe formula heating module；The syringe needle is consolidated Fixed set is relatively fixed setting with the straight-line guidance track, and sliding block is slidably disposed on straight-line guidance track；Sliding block and institute State syringe needle fixed cover to be oppositely arranged, the syringe needle fixed cover is connected with one end of the body, sliding block is another with the body End connection.

8. Artificial Insemination For Honey Bees syringe needle drawing device according to claim 7, it is characterised in that the draw-off gear with it is described Sliding block is connected, and passes through body described in the sliding block pull.

9. a kind of syringe needle drawing method of the Artificial Insemination For Honey Bees syringe needle drawing device described in use claim any one of 1-8, its It is characterised by possessing and comprise the following steps：

Step one, one end of the body is passed through to the through hole of described sleeve pipe formula heating module, the syringe needle is fixed on and consolidates Surely put；

Step 2, opens the low-tension supply, and carrying out heating to the body using described sleeve pipe formula heating module makes the pipe Body melts；

Step 3, uniformly firmly stretching the body makes body taper to setting caliber, and needle blanks are made.

10. syringe needle drawing method according to claim 9, it is characterised in that the front end of the needle blanks is cut off and made Into tip-like；Bell mouth shape is made in the rear end of the needle blanks.