CN106857674B - Application of bacillus methylotrophicus BH21, microbial fertilizer and preparation method thereof - Google Patents
Application of bacillus methylotrophicus BH21, microbial fertilizer and preparation method thereof Download PDFInfo
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- CN106857674B CN106857674B CN201710070295.3A CN201710070295A CN106857674B CN 106857674 B CN106857674 B CN 106857674B CN 201710070295 A CN201710070295 A CN 201710070295A CN 106857674 B CN106857674 B CN 106857674B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
本发明公开了保藏号为CGMCC No.11840的甲基营养型芽孢杆菌BH21在制备具有促长和提高植物耐盐性的微生物肥料中的应用，并公开了所述微生物肥料包括畜禽粪便、蘑菇渣、养殖垫料和甲基营养型芽孢杆菌BH21，其制备方法为：将甲基营养型芽孢杆菌BH21、腐熟粪便混匀后，采用条式堆垛发酵方式进行发酵，连续发酵4‑5天，发酵过程中每天翻堆，控制堆温不超过45℃，发酵完成后，获得所述的微生物肥料。本发明菌株具有较强的耐盐性，且能够促进植物生根和生长，其和腐熟有机肥组合成生物有机肥能够为植物生长提供丰富的营养，而有机肥的营养也为功能菌株的存活和定殖提供了良好的营养。The invention discloses the application of methylotrophic bacillus BH21 with the preservation number of CGMCC No. 11840 in the preparation of microbial fertilizers with the ability to promote growth and improve plant salt tolerance, and discloses that the microbial fertilizers include livestock and poultry manure, mushrooms Slag, culture bedding and Bacillus methylotroph BH21, the preparation method is as follows: after mixing Bacillus methylotroph BH21 and decomposed feces, adopt strip stacking fermentation mode to ferment, and ferment continuously for 4-5 days , during the fermentation process, the heap is turned every day, and the temperature of the heap is controlled not to exceed 45°C. After the fermentation is completed, the microbial fertilizer is obtained. The strain of the invention has strong salt tolerance, and can promote the rooting and growth of plants. The bio-organic fertilizer combined with the decomposed organic fertilizer can provide abundant nutrition for plant growth, and the nutrition of the organic fertilizer is also for the survival and growth of functional strains. Colonization provides good nutrition.
Description
Technical field
The present invention relates to microorganism fungus kind applied technical fields, and in particular to a kind of Methylotrophic bacillus BH21's
Using and microbial manure prepared therefrom and preparation method thereof.
Background technique
In recent years, facility plantation development in China's is rapid.410.9 ten thousand hectares of China's installment agriculture growth area in 2015, accounts for
85% or so of world's industrialized agriculture gross area.But with the fast development of facility cultivation, long-term ground mulching cultivation makes soil
The physicochemical character and biological characteristics of earth have changed a lot, and the earth's surface accumulation phenomenon of especially soil salt is obvious, soil
Earth secondary salinization problem is universal.The soil of secondary salinization occurs in addition to the growth and development of Restraining culture crop, also causes it
The decline of yield and quality.Soil in protected field secondary salinization has become one of generation soil swelling-shrinkage in current facility vegetable cultivation
Main Factors.How the agrological production capacity that is healthy, improving secondary salinization soil of support facility is to ensure that China's agriculture
The key of industry sustainable development.
Microorganism is the important activity factor in soil environment, is improving soil microenvironment, is improving crop production energy
It is played an important role in terms of power.It is effective that the bacterial strain with raising plant salt tolerance and growth ability and exploitation, which are screened, with the bacterial strain
It is used in industrialized agriculture production, is reduced since salt content is high in facility canopy planting soil by the biological organic fertilizer of ingredient
Caused by crop growth inhibit, have the function that promote plant growth, improve plant salt endurance.
Application No. is a kind of 201610720403.2 application for a patent for invention " salt tolerant bacterium T5S2 and its in microbial manure
In application " disclose salt tolerant bacterium T5S2 and be formed by the salt reduction of cotton, corn that microbial manure plants salt-soda soil, increase
Production effect.Application No. is 201410623410.1 applications for a patent for invention " a kind of salt-durable microbe flora and its application " to disclose
A kind of application of the salt tolerant compounding microbial flora in the waste drilling mud and oily sludge for handling alkali content with high salt.Application number
A kind of utilize again is disclosed for a kind of 201310466328.8 application for a patent for invention " method that microorganism improves salt affected soil "
The method for closing microbial bacterial agent improvement salt affected soil.
However, at present about Methylotrophic bacillus as salt tolerant bacterium carry out facility salinization soil improvement and
Research in terms of plant growing is less, promotes plant growth and the research of the Methylotrophic bacillus to take root not to have also to having
Have.
Summary of the invention
Based on the current saliferous status of China's soil in protected field, the object of the present invention is to provide Methylotrophic bacillus
Application of the BH21 in terms of promoting plant growth, taking root and improve salt resistance ability, and providing by the bacterial strain is the micro- of effective component
Bio-feritlizer and preparation method thereof, and it is used for the plantation of alkaline land improving and industrialized agriculture.
The technical solution adopted by the invention is as follows:
Methylotrophic bacillus BH21 is promoting plant growth and is improving the application in plant salt endurance, major embodiment
The growth of plant when promoting plant establishment and improving salt stress.The Methylotrophic bacillus BH21 was in 2015
December 09 has carried out preservation, deposit number CGMCC in China Committee for Culture Collection of Microorganisms's common micro-organisms center
No.11840, and patent of invention Shen was had submitted to Patent Office, Intellectual Property Right Bureau of the RPC on 03 07th, 2016
Please, application No. is 201610126777 .1.
Have the present invention also provides Methylotrophic bacillus BH21 in preparation and promotes plant growth and raising plant
Application in the microbial manure of salt tolerance, it is preferable that the bacterium of Methylotrophic bacillus BH21 in the microbial manure
Number is more than or equal to 100,000,000 cfu/ grams.
Further, the present invention also provides the microbial manures comprising Methylotrophic bacillus BH21 comprising
Following effective component: feces of livestock and poultry, mushroom residue, cultivates padding and Methylotrophic bacillus BH21 at potato slag, wherein mass ratio
Feces of livestock and poultry: potato slag+mushroom residue+cultivation padding=50-60:40-50, potato slag, mushroom residue and cultivation padding can adaptation to local conditions, press
It is mixed according to arbitrary proportion, the bacterium number of Methylotrophic bacillus BH21 is more than or equal to 100,000,000 cfu/ grams in the microbial manure.
Further, the present invention also provides the preparation methods of mentioned microorganism fertilizer, it may be assumed that the feces of livestock and poultry, potato
Slag, mushroom residue and cultivation padding carry out decomposed, addition Methylotrophic bacillus when decomposed heap temperature drop is to 45 DEG C or less
BH21, daily turning is primary, controls heap temperature at 45 DEG C hereinafter, up to the microbial manure after 4-5 days.
The beneficial effects of the present invention are: the present invention provides the new opplication of Methylotrophic bacillus BH21, the bacterial strain
It can promote plant establishment and growth, and the salt tolerance of plant can be improved.In addition, the bacterial strain can be big in salt affected soil
Amount breeding, forms dominant microflora, so that plant nutrient be promoted to absorb, increases plant products.In addition, the bacterium and decomposed manure group
Synthesising biological organic fertilizer can provide nutrition abundant for plant growth, and the nutrition of organic fertilizer be also function stem survival and
It colonizes and provides good nutrition.
Specific embodiment
1 Methylotrophic bacillus BH21 of embodiment acts on the growth-promoting root of in vitro cucumber leaves
Healthy leaves similar in size are won from healthy cucumber plant, with 4 times, 5 times and 10 times of distilled water prepared and diluted
Methylotrophic bacillus BH21 bacterium solution, by cucumber leaves immerse dilution in soak 5 min after, dry；Use distilled water
The cucumber leaves dried are soaked to compare.Processed cucumber leaves are placed on to the culture for being lined with wet gauze and appropriate distilled water
In ware, 22 DEG C of moisturizing cultures observe leaf growth state.
After moisturizing 4 days, all gone out with the cucumber leaves petiole base of Methylotrophic bacillus BH21 fermentation liquor treatment
It now takes root phenomenon, wherein the most obvious with the fermentation liquid rooting efficiency for diluting 4 times, number of averagely taking root is 12, root long
21.5cm；The cucumber leaves of the fermentation liquor treatment of 5 times of dilution are averagely taken root number 6, root long 12.3cm；The fermentation liquid of 10 times of dilution
It is few to handle number of taking root, and root is shorter, only 5.8cm.This may be since Methylotrophic bacillus BH21 was grown
Cheng Zhonghui generates some bioactive substances, to promote the petiole of cucumber leaves to be induced during moisturizing and take root.
In addition, leaf color is still bud green, vane extension, and clear water control treatment leaf color is partially yellow after fermentation liquid impregnate leaf in vitro 7 days
And wilt, handle ageing state.
2 Methylotrophic bacillus BH21 of embodiment acts on the growth-promoting root of in vitro tomato leaf
Healthy leaves similar in size are won from healthy tomato plant, with 5 times and 10 times of first of distilled water prepared and diluted
Tomato leaf is immersed in dilution after soaking 5 min, is dried by base auxotype bacillus BH21 bacterium solution；With distillation water-soaked
The tomato leaf dried compares.Tomato leaf is placed in the culture dish for being lined with wet gauze and appropriate distilled water, 22 DEG C of guarantors
Leaf growth state is observed in wet culture.
After moisturizing 4 days, with the tomato leaf of the 5 times and 10 times fermentation liquor treatments of dilution of Methylotrophic bacillus BH21
Piece leaf color is bud green, open and flat, and all processing do not occur phenomenon of taking root.The discovery of tomato petiole base is observed at moisturizing 7 days, 5 times dilute
There is fibrous root in the tomato leaf for releasing liquid and the processing of 10 times of dilutions, and the fibrous root number of 5 times of dilutions processing is more, growth is strong
It is strong, and clear water control then goes out without root long.The discovery of different disposal leaf color is compared, the tomato leaf leaf color of clear water processing is yellow,
And it is bud green to also maintain blade with the tomato leaf that 5 times and 10 times dilutions of bacterium solution handle 7 days.
Influence of the 3 Methylotrophic bacillus BH21 of embodiment to plant salt endurance
Using hole plate seedling growth method, by the cucumber seeds of the green small garden of saliva, nursery to seedling grew to 1 heart stage of 1 leaf in the greenhouse,
The root of seedling, which cleans up, to be moved into water planting box (the long 20cm of box, width 15cm, high 10cm), simultaneously using the cystosepiment with a thickness of 1cm
The circular hole of drill straight diameter 0.5cm on it plants cucumber seedling onto cystosepiment, and each water planting box plants 9 plants of cucumber, uniformly arranges
Column.MM nutrient solution is filled it up in water planting box, and the processing of salt and bacterium is carried out when cucumber grows to two leaves wholeheartedly.Test is set altogether at four
Reason, processing 1 is the NaCl for being 100mmol/L containing concentration in MM nutrient solution；Processing 2 is in MM nutrient solution is containing concentration
The NaCl of 100mmol/L is simultaneously 5 × 10 containing concentration6The BH21 of cfu/mL；Processing 3 is MM nutrient solution.Cucumber is trained in plant
It supports in room (light: dark period=12:12,24 ± 2 DEG C of temperature) culture 14 days, the salt gypsum rock and growing state of cucumber is surveyed
It is fixed.It the results are shown in Table 1, by result it can be seen that BH21, which is added, can significantly reduce harm of the salt to cucumber.
Influence of 1 BH21 of table to salt resistance of plants
。
Embodiment 4 is using Methylotrophic bacillus BH21 as the bio-organic fertilizer of function stem and preparation method
The culture of Methylotrophic bacillus BH21 is using a kind of patent (methyl battalion for producing a variety of lipopeptid class antibiotics
It supports type bacillus and its using culture medium and cultural method 201610126777.1), is sent out using multistage fermentor
Ferment, spray-dried acquisition viable count are 5 × 1010Cfu/ grams of bacterium powder cryo-conservation is spare.
Specific step is as follows for bio-organic fertilizer: by pig manure, oyster mushroom slag (carbon-nitrogen ratio 33:1), (padding is former for pig raising padding
Material are as follows: sawdust+corn stover, pig raising are mixed using rear carbon-nitrogen ratio 30:1) 50:30:20 in mass ratio, adjust moisture content
In 60%-70%, decomposing microbial inoculum is added by 1% inoculum concentration, progress high temperature is decomposed, and heap temperature drop presses quality as low as 45 DEG C or less later
The BH21 bacterium powder that additional proportion is 0.1%, bacterium powder are diluted with 5% molasses, are uniformly sprayed on stacking with sprayer, are carried out later
Turning, daily turning is primary, altogether turning 5 days, detects the quantity of the BH21 in fertilizer, reaches 1.6 × 108Cfu/ grams.
Application of 5 bio-organic fertilizer containing BH21 of embodiment in soil salinization brooder
The application of BH21 bio-organic fertilizer, mu dosage 200kg, whole are carried out in the plastic warm shed of continuous plantation 5 years
It applies and is turned over into soil before ground.Application test sets 3 processing altogether: processing 1 applies BH21 biological organic fertilizer 200kg, processing 2 as mu
Mu applies 200 kg of organic fertilizer without BH21, and processing 3 compares for conventional compound fertilizer (nitrogen-phosphorus-potassium compound fertilizer).It is right in growing process
The growing state and yield of cucumber are observed and are measured, and the results are shown in Table 2.It can be seen from the results that BH21 biological organic fertilizer exists
Soil occurs to apply in saliferous brooder, can effectively promote cucumber growth and output increased.
Influence of the 2 BH21 biological organic fertilizer of table to cucumber growth and yield
。
The present invention lists 5 embodiments only to illustrate its content, and is not the exhaustion to embodiment.For affiliated neck
It is any on the basis of the above description not have creative improvement for the technical staff in domain, also all in protection of the invention
Among scope.There is no necessity and possibility to exhaust all the enbodiments.Therefore, spirit and model of the invention are not being departed from
Lower made equal transformation and modification are enclosed, all should be contained within the scope of the invention.
Claims (8)
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