CN106832001B - 一种杀虫融合蛋白、编码基因及其应用 - Google Patents
一种杀虫融合蛋白、编码基因及其应用 Download PDFInfo
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- C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
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Abstract
本发明公开了一种杀虫融合蛋白，所述杀虫融合蛋白包括IPD072蛋白和Vip3蛋白。本发明所设计的IPD072蛋白与一个Vip3毒素融合成的人工蛋白质分子，与原先的IPD072蛋白和Vip3蛋白相比，具有如下优点：杀虫谱广，能同时防治鳞翅目和鞘翅目中多种重要害虫(如甜菜夜蛾、粘虫、玉米根虫)，杀虫效率高达50％‑100％；有利于具有上述不同功能的蛋白和其它抗虫蛋白(如ICPs)联合使用，进一步扩大杀虫谱，延缓害虫抗性产生。
Description
(一)技术领域
本发明涉及一种杀虫融合蛋白、该杀虫融合的编码基因，及上述融合蛋白的应用。
(二)背景技术
害虫给全球农业生产带来巨大的损失，目前害虫防治主要依靠化学农药，但是农药的使用加重了生产成本，并且农药残留对人体健康带来严重危害。因此，利用基因工程方法防治害虫具有重大经济、环境和社会价值。
获得转基因抗虫作物的关键是克隆优良杀虫蛋白质。杀虫蛋白质有很多，使用得最为广泛的是苏云金芽孢杆菌(Bacillus thuringiensis，简称Bt)在产胞期间所分泌一种伴胞晶体蛋白(insecticidalcrystal proteins，ICP)，如Cry1Ab，Cry1C等，这些蛋白对鳞翅目、双翅目、鞘翅目等昆虫(比如小菜蛾、玉米螟)有很强的杀伤作用(Schnepf, E.,Crickmore,N.,Van,R.J.,Lereclus,D.,Baum,J.,&Feitelson,J.,et al.1998,62(3),775-806.)，目前已被深入研究和广泛使用，玉米、大豆、土豆、棉花等转Bt基因作物已得到大规模商业化种植。苏云金芽胞杆菌在营养生长期间，还会分泌一种与ICPs 无氨基酸序列同源性、杀虫机理完全不同的蛋白，即营养期杀虫蛋白(vegetative insecticidalproteins，VIPs)，如Vip3A、Vip3B、Vip3C、Vip3D、Vip3H，对一些对ICPs 不敏感的害虫也有杀虫活性，并且不会发生交叉抗性(Estruch,J.J.,Warren,G.W., Mullins,M.A.,Nye,G.J.,Craig,J.A.,&Koziel,M.G.1996,Proceedings of the National Academy ofSciences of the United States of America,93(11),5389-94.)。
Vip3蛋白作为一种新型的杀虫蛋白，对目前使用最为广泛的ICP蛋白从杀虫机制、杀虫谱到杀虫活性上都是一个很好的补充。比如，ICPs对斜纹夜蛾和粘虫的杀伤作用很弱，Vip3则对斜纹夜蛾和粘虫有非常好的防治效果。尽管如此，在转基因作物研发的实际操作中，仍然存在一些急需解决的技术性问题。例如，研究人员发现高表达单独的Vip3基因的植株生长比较迟缓，容易出现白化现象。这可能是由于转基因植物中直接表达Vip3蛋白后，该蛋白由于信号肽的分泌作用大量聚集并结合在植物细胞膜上并形成孔道，从而对细胞造成损坏，影响其生长发育。
玉米是全世界产量最高的粮食作物，同时也面临着诸多害虫的威胁。而在这些害虫当中，根萤叶甲的名字尤其让人胆寒——它们是破坏力最强的玉米害虫之一。根萤叶甲的幼虫侵入寄主的根茎后，会吞食组织，导致根腐烂、植株倒伏，使得植株减产乃至死亡。而成虫也会采食寄主的叶、花以及种子。在1955年以前，它们仅分布在美国有限的几个州内，但在这之后，它们侵入了本不太适合它们生存的美国北部。1990 年，东欧激荡，这种害虫就在这个时候被意外引入塞尔维亚，于是，欧洲大陆尤其是东欧，也成了它们的地盘。而近些年也有蔓延至中国的境内。根萤叶甲是根萤叶甲属 (Diabrotica sp.)昆虫的统称。在美国，危害玉米的几种根萤叶甲被称作玉米根虫(Corn Rootworm)，其中危害最大的三种被叫做“西方玉米根虫”、“北方玉米根虫”及“南方玉米根虫”。目前发现的具有玉米根虫杀灭效果的蛋白主要是来自苏云金杆菌中的Cry 蛋白和Vip蛋白。其中，mCry3A、Cry3Bb1、eCry3.1Ab和蛋白复合体 Cry34Ab1/Cry35Ab1已经在商业化的转基因品系中应用，并且已经产生了不同程度的抗性(Lefko S A,Nowatzki T M,Thompson S D,et al.2008,Journalof Applied Entomology,132(3),189–204；Zhao,J.Z.,Oneal,M.A.,Richtman,N.M.,Thompson,S. D.,Cowart,M.C.,&Nelson,M.E.,et al.2016,Journal of EconomicEntomology(3))。也有研究表明，RNAi技术可以用于玉米根虫等鞘翅目害虫的防治(Baum,J.A.,Bogaert, T.,Clinton,W.,Heck,G.R.,Feldmann,P.,&Ilagan,O.,et al.2007,Nature Biotechnology,25(11),1322-6.)。最近，杜邦公司从假单胞菌(Pseudomonas)中克隆的一个基因IPD072Aa具有杀灭玉米根虫的效果(Schellenberger,U.,Oral,J.,Rosen,B.A.,Wei,J.Z.,Zhu,G.,&Xie,W.,et al.2016,Science,354(6312),635-637.)。
第一代转基因作物大多只具有抗鳞翅目害虫的特性，而第二代转基因作物正在向同时抗多种害虫的复合性状发展。获得具有复合性状的转基因作物有多种方法，比如通过对具有单一性状的转基因作物的杂交；通过将多个基因表达框构建在同一个表达框里面；通过共转化，将多个含有单基因的农杆菌混在一起，进行混合转化，筛选出多个基因同时整合了两个或多个质粒T-DNA的转基因植株。但是上述方法在实际操作和应用中都还存在一些问题。因此，获得更为简单高效的培育具有复合性状的转基因作物的方法是当下需要切实解决的问题。
(三)发明内容
本发明目的是提供一种通过基因融合使得同一个杀虫融合蛋白同时对鳞翅目和鞘翅目害虫具有杀伤效果的方法。
本发明采用的技术方案是：
本发明提供一种杀虫融合蛋白，所述杀虫融合蛋白从N端到C端分别为IPD072 蛋白和Vip3毒素。
进一步，所述IPD072蛋白为IPD072Aa蛋白、IPD072Bb蛋白、IPD072Ca蛋白或IPD072Da蛋白之一；所述Vip毒素为Vip3A毒素、Vip3B毒素、Vip3C毒素、Vip3D 毒素或Vip3H毒素中的一种。
进一步，所述杀虫融合蛋白由IPD072Aa蛋白和Vip3A毒素融合而成，氨基酸序列为SEQ ID NO：2所示。
进一步，所述杀虫融合蛋白由IPD072Aa蛋白和Vip3H毒素融合而成，氨基酸序列为SEQ ID NO：4所示。
本发明还提供一种所述杀虫融合蛋白的编码基因，所述的基因从5’-3’依次为编码 IPD072蛋白的核苷酸序列和编码Vip3毒素的核苷酸序列；且上述2个核苷酸序列位于同一个开放阅读框内。
进一步，所述的编码IPD072蛋白的基因为IPD072Aa、IPD072Bb、IPD072Ca、IPD072Da之一；所述编码Vip3毒素的基因为Vip3A、Vip3B、Vip3C、Vip3D或Vip3H 之一。更优选，所述编码基因的核苷酸序列为SEQ ID NO：1或SEQ ID NO：3所示。
本发明还提供一种所述的杀虫融合蛋白在制备转基因抗虫作物、转基因杀虫微生物或抗体中的应用。
进一步，所述转基因抗虫作物是由融合蛋白联合BT晶体毒素转化制备而成。
与现有技术相比，本发明有益效果主要体现在：本发明所设计的IPD072蛋白与一个Vip3毒素融合成的人工蛋白质分子，与原先的IPD072蛋白和Vip3蛋白相比，具有如下优点：杀虫谱广，能同时防治鳞翅目和鞘翅目中多种重要害虫(如甜菜夜蛾、粘虫、玉米根虫)，杀虫效率高达50％-100％；有利于具有上述不同功能的蛋白和其它抗虫蛋白(如ICPs)联合使用，进一步扩大杀虫谱，延缓害虫抗性产生。
(四)附图说明
图1是本发明的杀虫融合蛋白的结构图，杀虫融合蛋白中N端为IPD072蛋白， C端为Vip3毒素。
图2是本发明中将杀虫融合蛋白基因导入植物中的表达框结构图；pUBI为玉米泛素启动子，杀虫融合蛋白基因为IPD072-Vip3毒素融合基因。
图3是本发明中将杀虫融合蛋白和ICPs联合导入植物中的表达框结构图；pUBI 为玉米泛素启动子，杀虫融合蛋白基因为IPD072-Vip3毒素融合基因，p35S为花椰菜花叶病毒35S启动子，ICPs为伴胞晶体蛋白编码基因。
(五)具体实施方式
下面结合具体实施例对本发明进行进一步描述，但本发明的保护范围并不仅限于此：
实施例1、IPD072Aa-Vip3A杀虫融合蛋白表达载体的构建
IPD072Aa和Vip3A杀虫蛋白的基因均由上海生工合成，其DNA序列分别为SEQ IDNO：5和中国专利200610049611.0中的SEQ ID NO：7，并克隆在pET28a表达载体的限制性内切酶BamHI和SacI位点之间。构建好的载体分别命名为 pET28a-IPD072Aa和pET28a-Vip3A。
IPD072Aa-Vip3A合成具体步骤如下：
1、以Vip3A(中国专利200610049611.0中的SEQ ID NO：7)作为模板进过PCR 获得Vip3A基因片段。引物为：Vip3A-F：5’CCCGGGAAGGGTGGAGGAATGAACAAGAACAACACCAAG和Vip3A-R： 5’CGAGCTCCTACTTGATGCTCACGTCGTAGAACTTCACGA。这两个引物分别包含限制性内切酶位点XmalI和ScaI的位点。
2、用XmalI和ScaI处理PCR产物，并与用相同的酶处理过的pET28a-IPD072Aa 载体连接。
3、转入大肠杆菌，获得质粒为pET28a-IPD072Aa-Vip3A。融合蛋白基因 IPD072Aa-Vip3A(SEQ ID NO：1)被克隆到了pET28a表达载体中，融合蛋白基因编码的融合蛋白IPD072Aa-Vip3A(SEQ ID NO：2)分子量大约为98kD。
实施例2、IPD072Aa-Vip3H融合蛋白表达载体的构建
IPD072Aa和Vip3H杀虫蛋白的基因均由上海生工合成，其DNA序列分别为SEQ IDNO：5和SEQ ID NO：6，并克隆在pET28a表达载体的限制性内切酶BamHI和 SacI位点之间。构建好的载体分别命名为pET28a-IPD072Aa和pET28a-Vip3H。
IPD072Aa-Vip3H合成具体步骤如下：
4、以Vip3H(SEQ ID NO：6)作为模板进过PCR获得Vip3A基因片段。引物为：Vip3H-F：5’CCCGGGAAGGGTGGAGGAATGAACAAGAACAACAGTAAG和 Vip3H-R：5’CGAGCTCCTACTTGATGCTGAAGTCCCTGAAGGTGATGT。这两个引物分别包含限制性内切酶位点XmalI和ScaI的位点。
5、用XmalI和ScaI处理PCR产物，并与用相同的酶处理过的pET28a-IPD072Aa 载体连接。
6、转入大肠杆菌，获得质粒为pET28a-IPD072Aa-Vip3H。融合蛋白基因 IPD072Aa-Vip3H(SEQ ID NO：3)被克隆到了pET28a表达载体中，融合蛋白基因编码的融合蛋白IPD072Aa-Vip3H(SEQ ID NO：4)分子量大约为98kD。
实施例3、杀虫蛋白的表达
利用通用标准方法将上述含融合蛋白基因的表达载体 (pET28a-IPD072Aa-Vip3A、pET28a-IPD072Aa-Vip3H、pET28a-IPD072Aa、 pET28a-Vip3A和pET28a-Vip3H)分别转入大肠杆菌BL21star。挑取单克隆菌落接种到100ml LB细菌培养液中，37℃震动培养至OD600＝0.6，加入IPTG到0.5mM，继续在相同条件下培育4小时。收集培养液5000g离心10分钟沉淀大肠杆菌细胞，然后弃上清收集沉淀。沉淀中加30毫升pH7.5、20mM Tris-HCL缓冲液，超声粉碎后即可用于杀虫活性的测定。
实施例4、IPD072Aa-Vip3A和IPD072Aa-Vip3H对多种鳞翅目和鞘翅目害虫具有杀伤作用
实施例3所获得的杀虫蛋白(IPD072Aa-Vip3A、IPD072Aa-Vip3H、IPD072Aa、 Vip3A和Vip3H)分别涂在昆虫人工饲料的表面，新生一龄幼虫用来进行杀虫性测定。阴性对照为pET28a空载体，准备方法与实施例3相同。杀虫活性结果如表1所示：
表1、融合蛋白的杀虫效率
| 粘虫 | 甜菜夜蛾 | 西方玉米根虫 | |
| IPD072Aa | 0％ | 0％ | 100％ |
| Vip3A | 100％ | 100％ | 0％ |
| Vip3H | 100％ | 100％ | 0％ |
| IPD072Aa-Vip3A | 100％ | 100％ | 100％ |
| IPD072Aa-Vip3H | 100％ | 100％ | 100％ |
| 阴性对照 | 0％ | 0％ | 0％ |
实施例5、IPD072Aa-Vip3A植物转化载体构建
以IPD072Aa-Vip3A(SEQ ID NO：1)作为模板经过PCR获得IPD072Aa-Vip3A 基因片段。引物为：IVA-F：5’AGGATCCAACAATGGGCATCACCGTGACCAAC和 IVA-R：5’CGAGCTCCTACTTGATGCTCACGTCGTAGAACTTC。这两个引物分别包含限制性内切酶位点BamH1和ScaI的位点。人工合成终止子序列ter(SEQ ID NO： 8)，5’端和3’端分别设置有ScaI和KpnI位点。把用BamH1和ScaI酶切后的 IPD072Aa-Vip3A和用ScaI和KpnI酶切后的终止子连入经BamH1和KpnI酶切的pCambia1300载体中，获得pCambia1300-IPD072Aa-Vip3A-ter。
pUBI为玉米泛素蛋白启动子，通过PCR获得。以商业玉米品种郑单958的基因组DNA为模板，通过PCR扩增获得pUBI。PCR反应条件为：95℃3分钟；95℃15 秒，68℃15秒，72℃2分钟，重复32个循环；然后72℃10分钟。将获得的大约2.0 Kb的PCR产物克隆到T-载体pMD19中。获得序列正确的克隆用于下面的试验。PCR 引物为pUBI-F(5’GAAGCTTGCATGCCTACAGTGCAGCGTGACCC)和pUBI-R(5’ GGGTGGATCCTCTAGAGTCGACCTGCAGAAGTAAC)，引物上分别设置了HindIII 和BamHI限制性内切酶酶切位点。
用HindIII和BamHI酶切pUBI，把酶切后的片段连入用相同酶切的 pCambia1300-IPD072Aa-Vip3A-ter载体中，获得终载体。这个载体命名为：pCambia1300-pUBI-IPD072Aa-Vip3A。
最后，通过电转的方法把上述T-DNA质粒转入农杆菌LBA4404中，通过含有 15μg/ml四环素和50μg/mL的卡那霉素的YEP固体培养基筛选出阳性克隆，并保菌，用于接下来的植物转化。
实施例6、IPD072Aa-Vip3H植物转化载体构建
以IPD072Aa-Vip3H(SEQ ID NO：3)作为模板经过PCR获得IPD072Aa-Vip3H 基因片段。引物为：IVH-F：5’AGGATCCAACAATGGGCATCACCGTGACCAAC和 IVH-R：5’CGAGCTCCTACTTGATGCTGAAGTCCCTGAAGG。这两个引物分别包含限制性内切酶位点BamH1和ScaI的位点。人工合成终止子序列ter(SEQ ID NO：8)， 5’端和3’端分别设置有ScaI和KpnI位点。把用BamH1和ScaI酶切后的 IPD072Aa-Vip3H和用ScaI和KpnI酶切后的终止子连入经BamH1和KpnI酶切的 pCambia1300载体中，获得pCambia1300-IPD072Aa-Vip3H-ter。
pUBI为玉米泛素蛋白启动子的获得方法同实施例5。
用HindIII和BamHI酶切pUBI，把酶切后的片段连入用相同酶切的 pCambia1300-IPD072Aa-Vip3H-ter载体中，获得终载体。这个载体命名为： pCambia1300-pUBI-IPD072Aa-Vip3H。
最后，通过电转的方法把上述T-DNA质粒转入农杆菌LBA4404中，通过含有 15μg/ml四环素和50μg/mL的卡那霉素的YEP固体培养基筛选出阳性克隆，并保菌，用于接下来的植物转化。
实施例7、IPD072Aa-Vip3A和ICPs蛋白植物转化载体构建
以Cry1Ab(SEQ ID NO：7)杀虫蛋白的基因由上海生工合成，5’端和3’端分别设置有BamH1和ScaI位点。人工合成终止子序列ter(SEQ ID NO：8)，5’端和3’端分别设置ScaI和EcoRI位点。把用BamHI和ScaI酶切后的Cry1Ab和用ScaI和 EcoRI酶切后的终止子连入经BamH1和EcoRI酶切的pCambia1300载体中，获得 pCambia1300-Cry1Ab-ter。
p35S启动子为花椰菜花叶病毒CaMV 35S启动子，由上海生工合成，序列如SEQ IDNO：9，5’端和3’端分别设置有KpnI和BamHI位点。
用BamH1和EcoRI酶切pCambia1300-Cry1Ab-ter，用KpnI和BamHI酶切p35S，把上述两个片段和经KpnI和EcoRI酶切的载体pCambia1300-IPD072Aa-Vip3A-ter进行三段连接，获得终载体。这个载体命名为： pCambia1300-pUBI-IPD072Aa-Vip3A-p35S-Cry1Ab。
最后，通过电转的方法把上述T-DNA质粒转入农杆菌LBA4404中，通过含有 15μg/ml四环素和50μg/mL的卡那霉素的YEP固体培养基筛选出阳性克隆，并保菌，用于接下来的植物转化。
实施例8、IPD072Aa-Vip3H和ICPs蛋白植物转化载体构建
以Cry1Ab(SEQ ID NO：7)杀虫蛋白的基因由上海生工合成，5’端和3’端分别设置有BamH1和ScaI位点。人工合成终止子序列ter(SEQ ID NO：8)，5’端和3’端分别设置ScaI和EcoRI位点。把用BamHI和ScaI酶切后的Cry1Ab和用ScaI和 EcoRI酶切后的终止子连入经BamH1和EcoRI酶切的pCambia1300载体中，获得 pCambia1300-Cry1Ab-ter。
p35S启动子为花椰菜花叶病毒CaMV 35S启动子，由上海生工合成，序列如SEQ IDNO：9，5’端和3’端分别设置有KpnI和BamHI位点。
用BamH1和EcoRI酶切pCambia1300-Cry1Ab-ter，用KpnI和BamHI酶切p35S，把上述两个片段和经KpnI和EcoRI酶切的载体pCambia1300-IPD072Aa-Vip3H-ter进行三段连接，获得终载体。这个载体命名为： pCambia1300-pUBI-IPD072Aa-Vip3H-p35S-Cry1Ab。
最后，通过电转的方法把上述T-DNA质粒转入农杆菌LBA4404中，通过含有 15μg/ml四环素和50μg/mL的卡那霉素的YEP固体培养基筛选出阳性克隆，并保菌，用于接下来的植物转化。
实施例9、转基因玉米
玉米的转化技术已经比较成熟。参考文献如:Vladimir Sidorov&David Duncan(in M.Paul Scott(ed.),Methods in Molecular Biology:Transgenic Maize,vol:526；YujiIshida, YukohHiei&Toshihiko Komari(2007)Agrobacterium-mediatedtransformation of maize. NatureProtocols2:1614-1622。基本方法如下：
取授粉后8-10天的Hi-II玉米穗，收集所有的未成熟胚(大小为1.0-1.5mm)。将实施例8中构建的含有T-DNA载体转化农杆菌获得的重组农杆菌与未成熟胚在共培养培养基上(MS+2mg/L 2,4-D+30g/L蔗糖+3g/L琼脂(sigma 7921)+40mg/L乙酰丁香酮)共培养2-3天(22℃)。转移未成熟胚到愈伤诱导培养基上(MS+2mg/L 2,4-D+30 g/L蔗糖+2.5g/Lgelrite+5mg/L AgNO3+200mg/L乙酰丁香酮)，28℃暗培养10-14天。将所有的愈伤转到带有2mM草甘膦的筛选培养基(与愈伤诱导培养基相同)上，28℃暗培养2-3周。转移所有的组织到新鲜含2mM草甘膦的筛选培养基上，28℃暗培养2-3 周。然后，转移所有筛选后成活的胚性组织到再生培养基(MS+30g/L蔗糖+0.5mg/L kinetin+2.5g/L gelrite+200mg/L乙酰丁香酮)上，28℃暗培养10-14天，每皿一个株系。转移胚性组织到新鲜的再生培养基上，26℃光照培养10-14天。转移所有发育完全的植株到生根培养基(1/2MS+20g/L蔗糖+2.5g/Lgelrite+200mg/L乙酰丁香酮) 上，26℃光照培养直到根发育完全。获得含上述转化载体的转基因玉米植株。
实施例10、转基因玉米可以杀虫
将实施例9制备的转基因玉米植株的T0代植株移栽到温室中，用商业化品种“郑丹958”的母本，“郑58”(Z58)的花粉进行授粉，收获T0代种子。然后将这些品系与商业化品种“郑丹958”的母本，“郑58”(Z58)进行回交转育，获得Z58近等位基因系。再对这些近等位基因系的抗虫 性进行比较分析。
我们对获得的98个转pCambia1300-IPD072Aa-Vip3A-ter载体的转基因株系(命名为IV3A)和105个转pCambia1300-IPD072Aa-Vip3H-ter载体的转基因株系(命名为IV3H)进行抗虫性测定，其杀虫效果效果如表2所示：
表2*：
*注：表2为对播种后30天的玉米叶片提取物进行不同害虫的杀虫效果检测。每个实验都至少设置3个重复。
对我们获得的69个转pCambia1300-IPD072Aa-Vip3A-p35S-Cry1Ab载体的转基因株系(命名为IV3A1Ab)和73个转pCambia1300-IPD072Aa-Vip3H-p35S-Cry1Ab载体的转基因株系(命名为IV3H1Ab)进行抗虫性测定，其杀虫效果效果如表3所示：
表3*：
| 害虫 | 杀虫率 |
| 玉米螟 | 100％ |
| 棉铃虫 | 100％ |
| 粘虫 | 100％ |
| 甜菜夜蛾 | 100％ |
| 西方玉米根虫 | 100％ |
*注：表3为对播种后30天的玉米叶片提取物进行不同害虫的杀虫效果检测。每个实验都至少设置3个重复。
最后，还需要注意的是，以上列举的仅是本发明的具体实施例。显然，本发明不限于以上实施例，还可以有许多变形。本领域的普通技术人员能从本发明公开的内容直接导出或联想到的所有变形，均应认为是本发明的保护范围。
SEQUENCE LISTING
<110> 浙江大学
<120> 一种杀虫融合蛋白、编码基因及其应用
<130>
<160> 9
<170> PatentIn version 3.5
<210> 1
<211> 2652
<212> DNA
<213> unknown
<220>
<223> 人工序列
<400> 1
atgggcatca ccgtgaccaa caacagcagc aacccgatcg aggtggccat caaccactgg 60
ggcagcgacg gcgacaccag cttcttcagc gtgggcaacg gcaagcagga gacctgggac 120
aggagcgaca gcaggggctt cgtgctgagc ctgaagaaga acggcgccca gcacccgtac 180
tacgtgcagg ccagcagcaa gatcgaggtg gacaacaacg ccgtgaagga ccagggcagg 240
ctgatcgagc cgctgagccg aggccccggg aagggtggag gaatgaacaa gaacaacacc 300
aagctgagca ccagggccct gccgagcttc atcgactact tcaacggcat ctacggcttc 360
gccaccggca tcaaggacat catgaacatg atcttcaaga ccgacaccgg cggcgacctg 420
accctggacg agatcctgaa gaaccagcag ctgctgaacg acatcagcgg caagctggac 480
ggcgtgaacg gcagcctgaa cgacctgatc gcccagggca acctgaacac cgagctgagc 540
aaggagatcc tgaagatcgc caacgagcag aaccaggtgc tgaacgacgt gaacaacaag 600
ctggacgcca tcaacaccat gctgagggtg tacctgccga agatcaccag catgctgagc 660
gacgtgatga agcagaacta cgccctgagc ctgcagatcg agtacctgag caagcagctg 720
caggagatca gcgacaagct ggacatcatc aacgtgaacg tgctgatcaa cagcaccctg 780
accgagatca ccccggccta ccagaggatc aagtacgtga acgagaagtt cgaggagctg 840
accttcgcca ccgagaccag cagcaaggtg aagaaggacg gcagcccggc cgacatcctg 900
gacgagctga ccgagctgac cggcctggcc aagagcgtgc cgaagaacga cgtggacggc 960
ttcgagttct acctgaacac cttccacgac gtgatggtgg gcaacaacct gttcggcagg 1020
agcgccctga agaccgccag cgagctgatc accaaggaga acgtgaagac cagcggcagc 1080
gaggtgggca acgtgtacaa cagcctgatc gtgctgaccc tgctgcaggc caaggccttc 1140
ctgaccctga ccacctgcag gaagctgctg ggcctggccg acatcgacta caccagcatc 1200
atgaacgagc acctgaacaa ggagaaggag gagttcaggg tgaacatccc gccgaccctg 1260
agcaacacct tcagcaaccc gaactacgcc aaggtgaagg gcagcgacga ggacgccaag 1320
atgatcgtgg aggccaagcc gggccacgcc ctggtgggct tcgagatcag caacgacagc 1380
atcaccgtgc tgaaggtgta cgaggccaag ctgaagcaga actaccaggt ggacaaggac 1440
agcctgagcg aggtgatcta cggcgacatg gacaagctgc tgggcccgga ccagagcggc 1500
ccgatctact acccgaacaa catcgtgttc ccgaacgagt acgtgatcac caagatcgac 1560
ttcaccaaga agatgaagac cctgaggtac gaggtgaccg ccaacttcta cgacagcagc 1620
accggcgaga tcgacctgaa caagaagaag gtggagagca gcgaggccga gtacaggacc 1680
ctgagcgcca acgacgacgg cgtgtacatg ccgctgggcg tgatcagcga gaccttcctg 1740
accccgatca acggcttcgg cctgcaggcc gacgagaaca gcaggctgat caccctgacc 1800
tgcaagagct acctgaggga gctgctgctg gccaccgacc tgagcaacaa ggagaccaag 1860
ctgatcgtgc cgccgagcgg cttcatcaag aacatcgtgg agaacggcag catcgaggag 1920
gacaacctgg agccgtggaa ggccaacaac aagaacgcct acgtggacca caccggcggc 1980
gtgaacggca ccaaggccct gtacgtgcac aaggacggcg gcatcagcca gttcatcggc 2040
gacaagctga agccgaagac cgagtacgtg atccagtaca ccgtgaaggg caagccgagc 2100
atccacctga aggacgagaa caccggctac atccactacg aggacaccaa caacaacctg 2160
gaggactacc agaccatcac caagaggttc accaccggca ccgacctgaa gggcgtgtac 2220
ctgatcctga agagccagaa cggcgacgag gcctggggcg acaacttcat catcctggag 2280
atcagcccga gcgagaagct gctgagcccg gagctgatca acaccaacaa ctggaccagc 2340
accggcagca ccaacatcag cggcaacacc ctgaccctgt accagggcgg caggggcatc 2400
ctgaagcaga acctgcagct ggacagcttc agcacctaca gggtgtactt cagcgtgagc 2460
ggcgacgcca acgtgaggat caggaacagc agggaggtgc tgttcgagaa gaggtacatg 2520
agcggcgcca aggacgtgag cgagatcttc accaccaagc tgggcaagga caacttctac 2580
atcgagctga gccagggcaa caacctgaac ggcggcccga tcgtgaagtt ctacgacgtg 2640
agcatcaagt ag 2652
<210> 2
<211> 883
<212> PRT
<213> unknown
<220>
<223> 人工序列
<400> 2
Met Gly Ile Thr Val Thr Asn Asn Ser Ser Asn Pro Ile Glu Val Ala
1 5 10 15
Ile Asn His Trp Gly Ser Asp Gly Asp Thr Ser Phe Phe Ser Val Gly
20 25 30
Asn Gly Lys Gln Glu Thr Trp Asp Arg Ser Asp Ser Arg Gly Phe Val
35 40 45
Leu Ser Leu Lys Lys Asn Gly Ala Gln His Pro Tyr Tyr Val Gln Ala
50 55 60
Ser Ser Lys Ile Glu Val Asp Asn Asn Ala Val Lys Asp Gln Gly Arg
65 70 75 80
Leu Ile Glu Pro Leu Ser Arg Gly Pro Gly Lys Gly Gly Gly Met Asn
85 90 95
Lys Asn Asn Thr Lys Leu Ser Thr Arg Ala Leu Pro Ser Phe Ile Asp
100 105 110
Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp Ile Met
115 120 125
Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp Leu Thr Leu Asp Glu
130 135 140
Ile Leu Lys Asn Gln Gln Leu Leu Asn Asp Ile Ser Gly Lys Leu Asp
145 150 155 160
Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn Leu Asn
165 170 175
Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln Asn Gln
180 185 190
Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr Met Leu
195 200 205
Arg Val Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val Met Lys
210 215 220
Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Lys Gln Leu
225 230 235 240
Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val Asn Val Leu Ile
245 250 255
Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln Arg Ile Lys Tyr
260 265 270
Val Asn Glu Lys Phe Glu Glu Leu Thr Phe Ala Thr Glu Thr Ser Ser
275 280 285
Lys Val Lys Lys Asp Gly Ser Pro Ala Asp Ile Leu Asp Glu Leu Thr
290 295 300
Glu Leu Thr Gly Leu Ala Lys Ser Val Pro Lys Asn Asp Val Asp Gly
305 310 315 320
Phe Glu Phe Tyr Leu Asn Thr Phe His Asp Val Met Val Gly Asn Asn
325 330 335
Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala Ser Glu Leu Ile Thr Lys
340 345 350
Glu Asn Val Lys Thr Ser Gly Ser Glu Val Gly Asn Val Tyr Asn Ser
355 360 365
Leu Ile Val Leu Thr Leu Leu Gln Ala Lys Ala Phe Leu Thr Leu Thr
370 375 380
Thr Cys Arg Lys Leu Leu Gly Leu Ala Asp Ile Asp Tyr Thr Ser Ile
385 390 395 400
Met Asn Glu His Leu Asn Lys Glu Lys Glu Glu Phe Arg Val Asn Ile
405 410 415
Pro Pro Thr Leu Ser Asn Thr Phe Ser Asn Pro Asn Tyr Ala Lys Val
420 425 430
Lys Gly Ser Asp Glu Asp Ala Lys Met Ile Val Glu Ala Lys Pro Gly
435 440 445
His Ala Leu Val Gly Phe Glu Ile Ser Asn Asp Ser Ile Thr Val Leu
450 455 460
Lys Val Tyr Glu Ala Lys Leu Lys Gln Asn Tyr Gln Val Asp Lys Asp
465 470 475 480
Ser Leu Ser Glu Val Ile Tyr Gly Asp Met Asp Lys Leu Leu Gly Pro
485 490 495
Asp Gln Ser Gly Pro Ile Tyr Tyr Pro Asn Asn Ile Val Phe Pro Asn
500 505 510
Glu Tyr Val Ile Thr Lys Ile Asp Phe Thr Lys Lys Met Lys Thr Leu
515 520 525
Arg Tyr Glu Val Thr Ala Asn Phe Tyr Asp Ser Ser Thr Gly Glu Ile
530 535 540
Asp Leu Asn Lys Lys Lys Val Glu Ser Ser Glu Ala Glu Tyr Arg Thr
545 550 555 560
Leu Ser Ala Asn Asp Asp Gly Val Tyr Met Pro Leu Gly Val Ile Ser
565 570 575
Glu Thr Phe Leu Thr Pro Ile Asn Gly Phe Gly Leu Gln Ala Asp Glu
580 585 590
Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys Ser Tyr Leu Arg Glu Leu
595 600 605
Leu Leu Ala Thr Asp Leu Ser Asn Lys Glu Thr Lys Leu Ile Val Pro
610 615 620
Pro Ser Gly Phe Ile Lys Asn Ile Val Glu Asn Gly Ser Ile Glu Glu
625 630 635 640
Asp Asn Leu Glu Pro Trp Lys Ala Asn Asn Lys Asn Ala Tyr Val Asp
645 650 655
His Thr Gly Gly Val Asn Gly Thr Lys Ala Leu Tyr Val His Lys Asp
660 665 670
Gly Gly Ile Ser Gln Phe Ile Gly Asp Lys Leu Lys Pro Lys Thr Glu
675 680 685
Tyr Val Ile Gln Tyr Thr Val Lys Gly Lys Pro Ser Ile His Leu Lys
690 695 700
Asp Glu Asn Thr Gly Tyr Ile His Tyr Glu Asp Thr Asn Asn Asn Leu
705 710 715 720
Glu Asp Tyr Gln Thr Ile Thr Lys Arg Phe Thr Thr Gly Thr Asp Leu
725 730 735
Lys Gly Val Tyr Leu Ile Leu Lys Ser Gln Asn Gly Asp Glu Ala Trp
740 745 750
Gly Asp Asn Phe Ile Ile Leu Glu Ile Ser Pro Ser Glu Lys Leu Leu
755 760 765
Ser Pro Glu Leu Ile Asn Thr Asn Asn Trp Thr Ser Thr Gly Ser Thr
770 775 780
Asn Ile Ser Gly Asn Thr Leu Thr Leu Tyr Gln Gly Gly Arg Gly Ile
785 790 795 800
Leu Lys Gln Asn Leu Gln Leu Asp Ser Phe Ser Thr Tyr Arg Val Tyr
805 810 815
Phe Ser Val Ser Gly Asp Ala Asn Val Arg Ile Arg Asn Ser Arg Glu
820 825 830
Val Leu Phe Glu Lys Arg Tyr Met Ser Gly Ala Lys Asp Val Ser Glu
835 840 845
Ile Phe Thr Thr Lys Leu Gly Lys Asp Asn Phe Tyr Ile Glu Leu Ser
850 855 860
Gln Gly Asn Asn Leu Asn Gly Gly Pro Ile Val Lys Phe Tyr Asp Val
865 870 875 880
Ser Ile Lys
<210> 3
<211> 2646
<212> DNA
<213> unknown
<220>
<223> 人工序列
<400> 3
atgggcatca ccgtgaccaa caacagcagc aacccgatcg aggtggccat caaccactgg 60
ggcagcgacg gcgacaccag cttcttcagc gtgggcaacg gcaagcagga gacctgggac 120
aggagcgaca gcaggggctt cgtgctgagc ctgaagaaga acggcgccca gcacccgtac 180
tacgtgcagg ccagcagcaa gatcgaggtg gacaacaacg ccgtgaagga ccagggcagg 240
ctgatcgagc cgctgagccg aggccccggg aagggtggag gaatgaacaa gaacaacagt 300
aagctctcca cccgcgccct cccgtccttc atcgactact tcaacggcat ctacggcttc 360
gccaccggca tcaaggacat catgaacatg atcttcaaga ccgacaccgg cggcaacgtc 420
accctcgacg agatcctcaa gaaccagcag ctcctcaacg agatcagcgg caagctcgac 480
ggcgtgaacg gctccctcaa cgagctgatc gcccaggtca acctcaacac cgagctgtcc 540
aaggagatcc tcaagatctc caacgagcag aaccaggtgc tcaacgacgt gaacaacaag 600
ctggacgcca tcaacaccat gctgcacatc tacctcccga agatcacctc catgctctcc 660
gacgtgatga agcagaacta cgccctctcc ctccagatcg agtacctctc caagcagctc 720
caggagatca gcgacaagct cgacatcatc aacgtgaacg tgctcatcaa ctccaccctc 780
accgagatca ccccggccta ccagcgcatc aagtacgtga acgagaagtt cgaggagctg 840
accttcgcca ccgagaccac cctcaaggtg aagaaggact cctccccggc cgacatcctc 900
gacgagctga ccgagctgac cgagctggcc aagtccgtga ccaagaacga cgtggacggc 960
ttcgagttct acctcaacac cttgcacgac gtgatggtgg gcaacaacct cttcggccgc 1020
tccgccctca agaccgcctc cgagctgatc gccaaggaga acgtgaagac ctccggctcc 1080
gaggtgggca acgtgtacaa cttcctcatc gtgctcaccg ccctgcaggc caaggccttc 1140
ctcaccctca ccacctgccg caagctcctc ggcctcgccg gcatcgacta cacctccatc 1200
atgaacgagc acctcaacaa ggagaaggag gagttccgcg tgaacatcct cccgaccctc 1260
tccaacacct tctccaaccc gaactacgcc aaggtgaagg gctccgacga ggacgccaag 1320
atgatcgtgg aggccaagcc gggccacgcc ctcgtgggct tcgagatgtc caacgactcc 1380
atcaccgtgc tcaaggtgta cgaggccaag ctcaagcaga actaccaggt ggacaaggac 1440
tccctctccg aggtgatcta cggcgacacc gacaagctct tctgcccgga ccagtccgag 1500
cagatatact acaccaacaa catcgtgttc ccgaacgagt acgtgatcac caagatcgac 1560
ttcaccaaga agatgaagac cctccgctac gaggtgaccg ccaacttcta cgactcctcc 1620
accggcgaga tcgacctcaa caagaagaag gtggagtcct ccgaggccga gtaccgcacc 1680
ctctccgcca acgacgacgg cgtgtacatg ccgctcggcg tgatctccga aaccttcctc 1740
accccgatca acggcttcgg cctccaggcc gacgagaact cccgcctcat caccctcacc 1800
tgcaagtcct acctccgcga gctgctcctc gccaccgacc tctccaacaa ggagaccaag 1860
ctcatcgtgc cgccgtccgg cttcatctcc aacatcgtgg agaacggcgg catcgaggag 1920
gacaacctcg agccgtggaa ggccaacaac aagaacgcct acgtggacca caccggcggc 1980
gtgaacggca ccaaggccct ctacgtgcac aaggacggcg gcttctccca gttcatcggc 2040
gacaagctca agccgaagac cgagtacgtg atccagtaca ccgtgaaggg caaggccagc 2100
atctacctga aggacgagaa gaacaacgag ggcatctacg aggagatcaa caacgacctg 2160
gaggacttcc agaccgtgac caagaggttc atcaccggca ccgacagcag cggcgtgcac 2220
ctgatcttca ccagccagaa cggcgacgag gccttcggcg gcaacttcat catcagcgag 2280
atcaggagca gcgaggagct gctgagcccg gagctgatca agagcgacgc ctgggtgggc 2340
agccagggca cctggatcag cggcaacagc ctgaccatca acagcaacgc caacggcacc 2400
ttcaggcaga acctgccgct ggagagctac agcacctaca gcatgaactt caacgtgaac 2460
ggcttcgcca aggtgaccgt gaggaacagc agggaggtgc tgttcgagaa gaacttcagc 2520
cagctgagcc cgaaggacta cagcgagaag ttcaccaccg ccgccaacaa caccggcttc 2580
tacgtggagc tgagcagggg cacccagggc ggcaacatca ccttcaggga cttcagcatc 2640
aagtag 2646
<210> 4
<211> 881
<212> PRT
<213> unknown
<220>
<223> 人工序列
<400> 4
Met Gly Ile Thr Val Thr Asn Asn Ser Ser Asn Pro Ile Glu Val Ala
1 5 10 15
Ile Asn His Trp Gly Ser Asp Gly Asp Thr Ser Phe Phe Ser Val Gly
20 25 30
Asn Gly Lys Gln Glu Thr Trp Asp Arg Ser Asp Ser Arg Gly Phe Val
35 40 45
Leu Ser Leu Lys Lys Asn Gly Ala Gln His Pro Tyr Tyr Val Gln Ala
50 55 60
Ser Ser Lys Ile Glu Val Asp Asn Asn Ala Val Lys Asp Gln Gly Arg
65 70 75 80
Leu Ile Glu Pro Leu Ser Arg Gly Pro Gly Lys Gly Gly Gly Met Asn
85 90 95
Lys Asn Asn Ser Lys Leu Ser Thr Arg Ala Leu Pro Ser Phe Ile Asp
100 105 110
Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp Ile Met
115 120 125
Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asn Val Thr Leu Asp Glu
130 135 140
Ile Leu Lys Asn Gln Gln Leu Leu Asn Glu Ile Ser Gly Lys Leu Asp
145 150 155 160
Gly Val Asn Gly Ser Leu Asn Glu Leu Ile Ala Gln Val Asn Leu Asn
165 170 175
Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ser Asn Glu Gln Asn Gln
180 185 190
Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr Met Leu
195 200 205
His Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val Met Lys
210 215 220
Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Lys Gln Leu
225 230 235 240
Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val Asn Val Leu Ile
245 250 255
Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln Arg Ile Lys Tyr
260 265 270
Val Asn Glu Lys Phe Glu Glu Leu Thr Phe Ala Thr Glu Thr Thr Leu
275 280 285
Lys Val Lys Lys Asp Ser Ser Pro Ala Asp Ile Leu Asp Glu Leu Thr
290 295 300
Glu Leu Thr Glu Leu Ala Lys Ser Val Thr Lys Asn Asp Val Asp Gly
305 310 315 320
Phe Glu Phe Tyr Leu Asn Thr Leu His Asp Val Met Val Gly Asn Asn
325 330 335
Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala Ser Glu Leu Ile Ala Lys
340 345 350
Glu Asn Val Lys Thr Ser Gly Ser Glu Val Gly Asn Val Tyr Asn Phe
355 360 365
Leu Ile Val Leu Thr Ala Leu Gln Ala Lys Ala Phe Leu Thr Leu Thr
370 375 380
Thr Cys Arg Lys Leu Leu Gly Leu Ala Gly Ile Asp Tyr Thr Ser Ile
385 390 395 400
Met Asn Glu His Leu Asn Lys Glu Lys Glu Glu Phe Arg Val Asn Ile
405 410 415
Leu Pro Thr Leu Ser Asn Thr Phe Ser Asn Pro Asn Tyr Ala Lys Val
420 425 430
Lys Gly Ser Asp Glu Asp Ala Lys Met Ile Val Glu Ala Lys Pro Gly
435 440 445
His Ala Leu Val Gly Phe Glu Met Ser Asn Asp Ser Ile Thr Val Leu
450 455 460
Lys Val Tyr Glu Ala Lys Leu Lys Gln Asn Tyr Gln Val Asp Lys Asp
465 470 475 480
Ser Leu Ser Glu Val Ile Tyr Gly Asp Thr Asp Lys Leu Phe Cys Pro
485 490 495
Asp Gln Ser Glu Gln Ile Tyr Tyr Thr Asn Asn Ile Val Phe Pro Asn
500 505 510
Glu Tyr Val Ile Thr Lys Ile Asp Phe Thr Lys Lys Met Lys Thr Leu
515 520 525
Arg Tyr Glu Val Thr Ala Asn Phe Tyr Asp Ser Ser Thr Gly Glu Ile
530 535 540
Asp Leu Asn Lys Lys Lys Val Glu Ser Ser Glu Ala Glu Tyr Arg Thr
545 550 555 560
Leu Ser Ala Asn Asp Asp Gly Val Tyr Met Pro Leu Gly Val Ile Ser
565 570 575
Glu Thr Phe Leu Thr Pro Ile Asn Gly Phe Gly Leu Gln Ala Asp Glu
580 585 590
Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys Ser Tyr Leu Arg Glu Leu
595 600 605
Leu Leu Ala Thr Asp Leu Ser Asn Lys Glu Thr Lys Leu Ile Val Pro
610 615 620
Pro Ser Gly Phe Ile Ser Asn Ile Val Glu Asn Gly Gly Ile Glu Glu
625 630 635 640
Asp Asn Leu Glu Pro Trp Lys Ala Asn Asn Lys Asn Ala Tyr Val Asp
645 650 655
His Thr Gly Gly Val Asn Gly Thr Lys Ala Leu Tyr Val His Lys Asp
660 665 670
Gly Gly Phe Ser Gln Phe Ile Gly Asp Lys Leu Lys Pro Lys Thr Glu
675 680 685
Tyr Val Ile Gln Tyr Thr Val Lys Gly Lys Ala Ser Ile Tyr Leu Lys
690 695 700
Asp Glu Lys Asn Asn Glu Gly Ile Tyr Glu Glu Ile Asn Asn Asp Leu
705 710 715 720
Glu Asp Phe Gln Thr Val Thr Lys Arg Phe Ile Thr Gly Thr Asp Ser
725 730 735
Ser Gly Val His Leu Ile Phe Thr Ser Gln Asn Gly Asp Glu Ala Phe
740 745 750
Gly Gly Asn Phe Ile Ile Ser Glu Ile Arg Ser Ser Glu Glu Leu Leu
755 760 765
Ser Pro Glu Leu Ile Lys Ser Asp Ala Trp Val Gly Ser Gln Gly Thr
770 775 780
Trp Ile Ser Gly Asn Ser Leu Thr Ile Asn Ser Asn Ala Asn Gly Thr
785 790 795 800
Phe Arg Gln Asn Leu Pro Leu Glu Ser Tyr Ser Thr Tyr Ser Met Asn
805 810 815
Phe Asn Val Asn Gly Phe Ala Lys Val Thr Val Arg Asn Ser Arg Glu
820 825 830
Val Leu Phe Glu Lys Asn Phe Ser Gln Leu Ser Pro Lys Asp Tyr Ser
835 840 845
Glu Lys Phe Thr Thr Ala Ala Asn Asn Thr Gly Phe Tyr Val Glu Leu
850 855 860
Ser Arg Gly Thr Gln Gly Gly Asn Ile Thr Phe Arg Asp Phe Ser Ile
865 870 875 880
Lys
<210> 5
<211> 261
<212> DNA
<213> unknown
<220>
<223> 人工序列
<400> 5
atgggcatca ccgtgaccaa caacagcagc aacccgatcg aggtggccat caaccactgg 60
ggcagcgacg gcgacaccag cttcttcagc gtgggcaacg gcaagcagga gacctgggac 120
aggagcgaca gcaggggctt cgtgctgagc ctgaagaaga acggcgccca gcacccgtac 180
tacgtgcagg ccagcagcaa gatcgaggtg gacaacaacg ccgtgaagga ccagggcagg 240
ctgatcgagc cgctgagcta g 261
<210> 6
<211> 2364
<212> DNA
<213> unknown
<220>
<223> 人工序列
<400> 6
atgaacaaga acaacagtaa gctctccacc cgcgccctcc cgtccttcat cgactacttc 60
aacggcatct acggcttcgc caccggcatc aaggacatca tgaacatgat cttcaagacc 120
gacaccggcg gcaacgtcac cctcgacgag atcctcaaga accagcagct cctcaacgag 180
atcagcggca agctcgacgg cgtgaacggc tccctcaacg agctgatcgc ccaggtcaac 240
ctcaacaccg agctgtccaa ggagatcctc aagatctcca acgagcagaa ccaggtgctc 300
aacgacgtga acaacaagct ggacgccatc aacaccatgc tgcacatcta cctcccgaag 360
atcacctcca tgctctccga cgtgatgaag cagaactacg ccctctccct ccagatcgag 420
tacctctcca agcagctcca ggagatcagc gacaagctcg acatcatcaa cgtgaacgtg 480
ctcatcaact ccaccctcac cgagatcacc ccggcctacc agcgcatcaa gtacgtgaac 540
gagaagttcg aggagctgac cttcgccacc gagaccaccc tcaaggtgaa gaaggactcc 600
tccccggccg acatcctcga cgagctgacc gagctgaccg agctggccaa gtccgtgacc 660
aagaacgacg tggacggctt cgagttctac ctcaacacct tgcacgacgt gatggtgggc 720
aacaacctct tcggccgctc cgccctcaag accgcctccg agctgatcgc caaggagaac 780
gtgaagacct ccggctccga ggtgggcaac gtgtacaact tcctcatcgt gctcaccgcc 840
ctgcaggcca aggccttcct caccctcacc acctgccgca agctcctcgg cctcgccggc 900
atcgactaca cctccatcat gaacgagcac ctcaacaagg agaaggagga gttccgcgtg 960
aacatcctcc cgaccctctc caacaccttc tccaacccga actacgccaa ggtgaagggc 1020
tccgacgagg acgccaagat gatcgtggag gccaagccgg gccacgccct cgtgggcttc 1080
gagatgtcca acgactccat caccgtgctc aaggtgtacg aggccaagct caagcagaac 1140
taccaggtgg acaaggactc cctctccgag gtgatctacg gcgacaccga caagctcttc 1200
tgcccggacc agtccgagca gatatactac accaacaaca tcgtgttccc gaacgagtac 1260
gtgatcacca agatcgactt caccaagaag atgaagaccc tccgctacga ggtgaccgcc 1320
aacttctacg actcctccac cggcgagatc gacctcaaca agaagaaggt ggagtcctcc 1380
gaggccgagt accgcaccct ctccgccaac gacgacggcg tgtacatgcc gctcggcgtg 1440
atctccgaaa ccttcctcac cccgatcaac ggcttcggcc tccaggccga cgagaactcc 1500
cgcctcatca ccctcacctg caagtcctac ctccgcgagc tgctcctcgc caccgacctc 1560
tccaacaagg agaccaagct catcgtgccg ccgtccggct tcatctccaa catcgtggag 1620
aacggcggca tcgaggagga caacctcgag ccgtggaagg ccaacaacaa gaacgcctac 1680
gtggaccaca ccggcggcgt gaacggcacc aaggccctct acgtgcacaa ggacggcggc 1740
ttctcccagt tcatcggcga caagctcaag ccgaagaccg agtacgtgat ccagtacacc 1800
gtgaagggca aggccagcat ctacctgaag gacgagaaga acaacgaggg catctacgag 1860
gagatcaaca acgacctgga ggacttccag accgtgacca agaggttcat caccggcacc 1920
gacagcagcg gcgtgcacct gatcttcacc agccagaacg gcgacgaggc cttcggcggc 1980
aacttcatca tcagcgagat caggagcagc gaggagctgc tgagcccgga gctgatcaag 2040
agcgacgcct gggtgggcag ccagggcacc tggatcagcg gcaacagcct gaccatcaac 2100
agcaacgcca acggcacctt caggcagaac ctgccgctgg agagctacag cacctacagc 2160
atgaacttca acgtgaacgg cttcgccaag gtgaccgtga ggaacagcag ggaggtgctg 2220
ttcgagaaga acttcagcca gctgagcccg aaggactaca gcgagaagtt caccaccgcc 2280
gccaacaaca ccggcttcta cgtggagctg agcaggggca cccagggcgg caacatcacc 2340
ttcagggact tcagcatcaa gtaa 2364
<210> 7
<211> 1848
<212> DNA
<213> unknown
<220>
<223> 人工序列
<400> 7
atggacaaca acccaaacat caacgaatgc attccataca actgcttgag taacccagaa 60
gttgaagtac ttggtggaga acgcattgaa accggttaca ctcccatcga catctccttg 120
tccttgacac agtttctgct cagcgagttc gtgccaggtg ctgggttcgt tctcggacta 180
gttgacatca tctggggtat ctttggtcca tctcaatggg atgcattcct ggtgcaaatt 240
gagcagttga tcaaccagag gatcgaagag ttcgccagga accaggccat ctctaggttg 300
gaaggattga gcaatctcta ccaaatctat gcagagagct tcagagagtg ggaagccgat 360
cctactaacc cagctctccg cgaggaaatg cgtattcaat tcaacgacat gaacagcgcc 420
ttgaccacag ctatcccatt gttcgcagtc cagaactacc aagttcctct cttgtccgtg 480
tacgttcaag cagctaatct tcacctcagc gtgcttcgag acgttagcgt gtttgggcaa 540
aggtggggat tcgatgctgc aaccatcaat agccgttaca acgaccttac taggctgatt 600
ggaaactaca ccgaccacgc tgttcgttgg tacaacactg gcttggagcg tgtctggggt 660
cctgattcta gagattggat tagatacaac cagttcagga gagaattgac cctcacagtt 720
ttggacattg tgtctctctt cccgaactat gactccagaa cctaccctat ccgtacagtg 780
tcccaactta ccagagaaat ctatactaac ccagttcttg agaacttcga cggtagcttc 840
cgtggttctg cccaaggtat cgaaggctcc atcaggagcc cacacttgat ggacatcttg 900
aacagcataa ctatctacac cgatgctcac agaggagagt attactggtc tggacaccag 960
atcatggcct ctccagttgg attcagcggg cccgagttta cctttcctct ctatggaact 1020
atgggaaacg ccgctccaca acaacgtatc gttgctcaac taggtcaggg tgtctacaga 1080
accttgtctt ccaccttgta cagaagaccc ttcaatatcg gtatcaacaa ccagcaactt 1140
tccgttcttg acggaacaga gttcgcctat ggaacctctt ctaacttgcc atccgctgtt 1200
tacagaaaga gcggaaccgt tgattccttg gacgaaatcc caccacagaa caacaatgtg 1260
ccacccaggc aaggattctc ccacaggttg agccacgtgt ccatgttccg ttccggattc 1320
agcaacagtt ccgtgagcat catcagagct cctatgttct catggattca tcgtagtgct 1380
gagttcaaca atatcattcc ttcctctcaa atcacccaaa tcccattgac caagtctact 1440
aaccttggat ctggaacttc tgtcgtgaaa ggaccaggct tcacaggagg tgatattctt 1500
agaagaactt ctcctggcca gattagcacc ctcagagtta acatcactgc accactttct 1560
caaagatatc gtgtcaggat tcgttacgca tctaccacaa acttgcaatt ccacacctcc 1620
atcgacggaa ggcctatcaa tcagggtaac ttctccgcaa ccatgtcaag cggcagcaac 1680
ttgcaatccg gcagcttcag aaccgtcggt ttcactactc ctttcaactt ctctaacgga 1740
tcaagcgttt tcacccttag cgctcatgtg ttcaattctg gcaatgaagt gtacattgac 1800
cgtattgagt ttgtgcctgc cgaagttacc ttcgaggctg agtactag 1848
<210> 8
<211> 208
<212> DNA
<213> unknown
<220>
<223> 人工序列
<400> 8
gagctctaga tgggccctgt tctgcacaaa gtggagtagt cagtcatcga tcaggaacca 60
gacaccagac ttttattcat acagtgaagt gaagtgaagt gcagtgcagt gagttgctgg 120
tttttgtaca acttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta 180
attcctaaaa ccaaaatcca ggggtacc 208
<210> 9
<211> 792
<212> DNA
<213> unknown
<220>
<223> 人工序列
<400> 9
ggtacctggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag 60
aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat 120
tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct 180
acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg 240
gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca 300
cgtcttcaaa gcaagtggat tgatgtgata acatggtgga gcacgacact ctcgtctact 360
ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa 420
gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa 480
ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta 540
tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca 600
tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct 660
ccactgacgt aagggatgac gcacaatccc actatccttc gcaagacctt cctctatata 720
aggaagttca tttcatttgg agaggacacg ctgaaatcac cagtctctct ctacaaatct 780
atctctggat cc 792
Claims (5)
1.一种杀虫融合蛋白，其特征在于所述杀虫融合蛋白从N端到C端依次为IPD072蛋白和Vip3毒素，所述杀虫融合蛋白为下列之一：（1）由IPD072Aa蛋白和Vip3A毒素融合而成，所述杀虫融合蛋白氨基酸序列为SEQ ID NO：2所示；（2）从N端到C端IPD072Aa蛋白和Vip3H毒素融合而成，所述杀虫融合蛋白氨基酸序列为SEQ ID NO：4所示。
2.一种权利要求1所述杀虫融合蛋白的编码基因，其特征在于所述的基因从5’-3’依次为编码IPD072蛋白的核苷酸序列和编码Vip3毒素的核苷酸序列；且上述2个核苷酸序列位于同一个开放阅读框内。
3.如权利要求2所述的编码基因，其特征在于所述编码基因的核苷酸序列为SEQ IDNO：1或SEQ ID NO：3所示。
4.一种权利要求1所述的杀虫融合蛋白在制备转基因抗虫作物、转基因杀虫微生物或抗体中的应用。
5.如权利要求4所述的应用，其特征在于所述转基因抗虫作物是由所述融合蛋白联合BT晶体毒素转化制备而成。
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| CN107723303A (zh) * | 2017-09-29 | 2018-02-23 | 杭州瑞丰生物科技有限公司 | 抗虫融合基因、编码蛋白、载体及其应用 |
| CN107828817B (zh) * | 2017-09-29 | 2021-02-19 | 杭州瑞丰生物科技有限公司 | 一种利用Bt蛋白防治农作物半翅目害虫的方法 |
| US11820791B2 (en) * | 2018-03-14 | 2023-11-21 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
| CA3093007A1 (en) * | 2018-04-27 | 2019-10-31 | Heather Marie CHRISTENSEN | Maize event dp-023211-2 and methods for detection thereof |
| CN115916983A (zh) * | 2020-06-03 | 2023-04-04 | 先锋国际良种公司 | 玉米事件dp-915635-4及其检测方法 |
| CN113793639B (zh) * | 2021-08-03 | 2024-01-05 | 杭州瑞丰生物科技有限公司 | 一种管理玉米螟对Bt毒素抗性的方法 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1818067A (zh) * | 2006-02-27 | 2006-08-16 | 浙江大学 | 抗虫融合基因、融合蛋白及其应用 |
| CN102031266A (zh) * | 2010-03-25 | 2011-04-27 | 浙江大学 | 抗虫融合基因、融合蛋白质及其应用 |
| CN102363631A (zh) * | 2011-11-09 | 2012-02-29 | 四川农业大学 | 一种杀虫Bt蛋白Cry8Qa1、其编码基因及应用 |
| CN105624177A (zh) * | 2016-02-04 | 2016-06-01 | 浙江大学 | 一种抗虫融合基因、编码蛋白、载体及其应用 |
-
2017
- 2017-01-21 CN CN201710046061.5A patent/CN106832001B/zh active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1818067A (zh) * | 2006-02-27 | 2006-08-16 | 浙江大学 | 抗虫融合基因、融合蛋白及其应用 |
| CN102031266A (zh) * | 2010-03-25 | 2011-04-27 | 浙江大学 | 抗虫融合基因、融合蛋白质及其应用 |
| CN102363631A (zh) * | 2011-11-09 | 2012-02-29 | 四川农业大学 | 一种杀虫Bt蛋白Cry8Qa1、其编码基因及应用 |
| CN105624177A (zh) * | 2016-02-04 | 2016-06-01 | 浙江大学 | 一种抗虫融合基因、编码蛋白、载体及其应用 |
Non-Patent Citations (2)
| Title |
|---|
| A selective insecticidal protein from Pseudomonas for controlling corn rootworms;Ute Schellenberger等;《Science》;20161104;第354卷(第6312期);摘要，第637页左栏第1段，Supplementary Materials部分表S1 * |
| Pseudomonas chlororaphis IPD072Aa gene, complete cds;Schellenberger,U.;《NCBI》;20160923;GenBank: KT795291.1 * |
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