CN106824006B - A kind of more reagent sequence feeding devices preventing cross contamination - Google Patents

Abstract

The invention provides a multi-reagent sequential liquid feeding device for preventing cross-contamination, comprising a housing and a multi-reagent sequential liquid feeding system; the multi-reagent sequential liquid feeding system is arranged in the housing; the multi-reagent sequential liquid feeding The system is a multi-layer structure, which at least includes a pipe connection layer, a reagent injection diversion layer, a waste liquid diversion layer, a cleaning liquid diversion layer and a reaction pool connection layer, and the pipe connection layer, reagent injection diversion layer, waste liquid The diversion layer, the cleaning liquid diversion layer, and the connection layer of the reaction pool are connected by radial pipes between adjacent two layers. The multi-reagent sequential liquid feeding device provided by the invention not only has no dead volume, but also effectively avoids reagent residue and cross-contamination when multiple reagents are fed into liquid.

Description

A multi-reagent sequential liquid feeding device to prevent cross-contamination

technical field

The invention relates to the field of liquid flow control, in particular to a multi-reagent sequential liquid feeding device for preventing cross-contamination.

Background technique

In the field of liquid flow control, especially in biochemical reaction systems, the sequential feeding of multiple reagents is a common liquid flow control step, but effectively avoiding cross-contamination of multiple reagents in the liquid flow control step is currently the most difficult solved problem.

U.S. Patent US09149803 and its continuation application patent CN102802402 in China provide a fluidic control system for sequential delivery of reagents, wherein the fluidic circuit for controlling multiple fluids includes a fluidic control node with an outlet, multiple fluidic an inlet, at least one waste port, and a plurality of channels each providing a different path for fluid communication between the at least one waste port and the fluidic junction, the plurality of channels Each of the fluid inlets is uniquely associated with one of the plurality of channels, each of the plurality of channels has a fluid resistance selected such that fluid only flows through a single fluid inlet and through an associated channel to leave the fluidic junction through one or more of the plurality of channels other than the associated channel to reach the at least one waste port such that any fluid from non-selected inlets is directed to the at least one waste port through one or more passages of the plurality of channels.

The principle of the fluidic system for the sequential delivery of reagents disclosed in the above-mentioned invention patents is to guide the reagents through the fluidic circuit to the common volume, and flow through other unselected inlets by means of liquid backflush, and then flow to the In the outer ring, there is a waste liquid outlet on one side of the ring; however, the setting of this waste liquid ring cannot effectively eliminate the dead volume, that is, in the actual implementation process, there may be a dead volume area in the waste liquid ring , cannot be rinsed clean, so there is a certain risk of cross-contamination of reagents.

Contents of the invention

Aiming at the defects existing in the prior art, the present invention provides a multi-reagent sequential liquid feeding device for preventing cross-contamination, which can meet the requirements of zero dead volume and no cross-contamination for the liquid feeding of various reagents.

In order to solve the above technical problems, the present invention provides a multi-reagent sequential liquid feeding device for preventing cross-contamination, which includes a housing and a multi-reagent sequential liquid feeding system; the multi-reagent sequential liquid feeding system is arranged in the housing;

The multi-reagent sequential liquid inlet system is a multi-layer structure, which at least includes a pipe connection layer 21, a reagent injection diversion layer 22, a waste liquid diversion layer 23, a cleaning solution diversion layer 24 and a reaction pool connection layer 25. The pipeline connection layer 21 , the reagent injection diversion layer 22 , the waste liquid diversion layer 23 , the cleaning solution diversion layer 24 , and the reaction pool connection layer 25 are all connected by axial pipes.

Preferably, the pipe connection layer 21 includes at least one cleaning solution inlet 2111 , multiple reagent inlets 2101 - 2110 and at least two waste solution outlets 2112 , 2113 .

Preferably, the cleaning liquid inlet 2111, the multiple reagent inlets 2101-2110, and the waste liquid outlets 2112 and 2113 can all be connected to external infusion pipes.

Preferably, the reagent injection guide layer 22 includes a plurality of reagent inflow holes 1 2201-2210, holes 2 2214-2223, holes 3 2211, holes 4 2212, holes 5 2213, node holes 2224, and holes connecting different holes. Diversion pipe.

Preferably, the waste liquid diversion layer 23 is provided with several holes, namely hole six 2311, first node hole 2312, hole eight 2314-2323, second node hole 2324, and diversion holes connecting different holes pipeline.

Preferably, hole nine 2401 , hole ten 2411 , hole eleven 2413 and hole twelve 2424 are provided on the cleaning fluid guide layer 24 , and hole nine 2401 and hole ten 2411 are connected through pipelines in the same layer.

Preferably, hole 13 2501 , hole 14 2513 , and hole 15 2524 are provided on the connection layer 25 of the reaction pool; the hole 13 2501 and hole 15 2524 are connected by pipes in the same layer.

Preferably, the reagent inlets 2101-2110 communicate with the first reagent inflow holes 2201-2210 through axial channels.

Preferably, the cleaning liquid inlet 2111 , hole three 2211 of the reagent injection guide layer 22 , hole six 2311 of the waste liquid guide layer 23 , and hole ten 2411 of the cleaning liquid guide layer 24 are correspondingly connected through axial channels.

Preferably, the waste liquid outlet 2112, the reagent injection hole 2212 of the diversion layer 22, and the first node hole 2312 of the waste liquid diversion layer 23 are correspondingly connected through an axial channel.

Preferably, the waste liquid outlet 2113, the fifth hole 2213 of the reagent injection guide layer 22, the seventh hole 2313 of the waste liquid guide layer 23, the eleventh hole 2413 of the cleaning liquid guide layer 24, and the hole 2413 of the reaction pool connection layer 25 The fourteenth hole 2513 communicates correspondingly through the axial channel.

Preferably, the second holes 2214-2223 of the reagent injection diversion layer 22 communicate with the eighth holes 2314-2323 of the waste liquid diversion layer 23 through axial channels.

Preferably, the reagent is injected into the junction hole 2224 of the diversion layer 22, the second junction hole 2324 of the waste liquid diversion layer 23, the hole 2424 of the cleaning liquid diversion layer 24, and the hole of the reaction pool connection layer 25. Fifteen 2524 are communicated correspondingly through the axial passage.

Preferably, the hole nine 2401 of the cleaning liquid guide layer 24 communicates with the hole thirteen 2501 of the reaction pool connection layer 25 correspondingly through an axial channel; the hole fifteen 2524 of the reaction pool connection layer 25 is connected with the inlet of the reaction pool, Hole fourteen 2513 is connected with the outlet of the reaction pool.

Preferably, the pipeline connection layer 21 , the reagent injection diversion layer 22 , the waste liquid diversion layer 23 , the cleaning reagent diversion layer 24 , and the reaction pool connection layer 25 are distributed sequentially up and down.

Preferably, the first holes 2201-2210 of the reagent injection guide layer 22 are equiangularly distributed around the node hole 2224; 2210 forms the outside of the circumference.

Preferably, the second holes 2214-2223 are respectively connected with the node holes 2224 through pipes, and the pipes are straight pipes or arc-shaped pipes; One side of the pipeline, and communicate with adjacent single pipelines through arc or straight pipelines.

Preferably, the first holes 2201-2210 correspond to the positions of the reagent inlets 2101-2110 of the pipeline connection layer 21, respectively.

Preferably, the eighth holes 2314-2323 of the waste liquid diversion layer 23 are equiangularly distributed around the second node hole, and correspond to the position of the second hole 2214-2223 of the reagent diversion layer 22, the second node The hole 2324 corresponds to the position of the node hole 2224 of the reagent guiding layer 22 ; the holes 2311 - 2313 correspond to the positions of the third hole 2211 and the fifth hole 2213 of the reagent guiding layer 22 .

Preferably, the eighth holes 2314-2323 communicate with the first junction hole 2312 through a straight guide pipe or an arc guide pipe.

Preferably, the liquid flow circuit formed by injecting the reagent into the diversion layer 22 is as follows: the cleaning liquid is injected from the cleaning liquid inlet 2111, and the cleaning liquid is divided into two parts. The second node hole 2324 reaches the node hole 2224. At this time, the cleaning solution will flow into the reagent injection guide layer from the node hole 2224, and flush to each channel that has not been injected. Reagent is injected into any hole in -2210, and the reagent enters the waste liquid diversion layer along with the cleaning liquid flowing through to achieve the purpose of cleaning the reagent injection port, and then close the cleaning liquid, at this time, the reagent flows into the second hole 2214-2223 and the node The pipeline connected to the hole 2224 will be divided into two parts, a part flows in the direction of the node hole 2224, and a part flows in the opposite direction; the reagent flowing in the direction of the node hole 2224 is divided into multiple directions, and a part of it breaks away from the flow guide layer 22 and enters the reaction pool , and the other part is diverted to each channel, and flows to the waste liquid diversion layer; finally, each reagent injection port is closed, and the cleaning liquid is opened to flush the pipeline.

Preferably, the liquid flow circuit formed by the waste liquid diversion layer 23 is as follows: all the liquid flowing in from the holes 2314-2323 of the waste liquid diversion layer 23 converges to the first node hole 2312 and excludes the system; the holes Any one of the eight holes 2314-2323 can be guided independently to the first node hole 2312 under the condition that the space structure allows, so as to reduce the resistance formed by the liquid flowing in from other holes.

Preferably, the liquid flow circuit formed by the cleaning solution diversion layer 24 is as follows: the hole ten 2411 of the cleaning solution diversion layer 24 communicates with the hole nine 2401 through a straight pipe or an arc-shaped pipe, and the liquid flowing in the hole ten 2411 diversion to hole nine 2401.

Preferably, the liquid flow circuit formed by the connection layer 25 of the reaction pool is as follows: the hole 13 2501 of the connection layer 25 of the reaction pool is connected with the hole 2504 through a pipeline, the hole 15 2524 is connected with the inlet of the reaction pool, and the hole 13 is connected to the inlet of the reaction pool. The liquid flowing in from 2501 is diverted to the reaction pool through hole 15 2524 , and into the pipeline formed by hole 15 2524 and hole 12 2424 .

Preferably, control valves are installed between the reagent inlets of the pipeline connection layer 21 and the reagent bottles to control the inflow and stop of the liquid or the outflow and stop, and all the control valves are controlled by the same control system.

The multi-reagent sequential liquid feeding system is used to realize zero dead volume cleaning of all channels in the system by injecting cleaning liquid. The steps are as follows: the cleaning liquid passes through the cleaning liquid inlet 2111, hole three 2211, hole six 2311, hole ten 2411, hole nine 2401, hole 13 2501 reaches hole 15 2524, at this time the liquid is divided into two parts, one part passes through the reaction tank, hole 14 2513, hole 11 2413, hole 7 2313, hole 5 2213 and reaches hole 2113 to discharge the system; A part of the liquid reaches the junction hole 2224 through the hole fifteen 2524, the twelve hole 2424, and the second junction hole 2324. At this time, the liquid will be divided into multiple parts at the plane of the reagent injection guide layer 22, and respectively pass through the second hole 2214 -2223 is discharged into the waste liquid diversion layer 23, and is diverted to the first node hole 2312 through the flat pipe of the waste liquid diversion layer 23, and discharged from the system through the first node hole 2312, hole four 2212, and waste liquid outlet 2112 .

Using the multi-reagent sequential liquid feeding system can realize the sequential liquid feeding of multiple reagents without cross-contamination by injecting multiple reagents. The steps are as follows: any reagent inlet in the pipeline connection layer 21, such as reagent inlet 2101, starts to feed liquid, and other Reagent inlets 2102-2110 stop liquid feeding, cleaning reagents stop liquid feeding, reagents enter reagent injection guide layer 22 through reagent inlet 2101 and hole 1 2201, and reagents flow into communication hole 2222 from hole 1 2201 at the plane of reagent injection guide layer 22 In the channel of the junction hole 2224, the liquid will be divided into two parts when just entering the passage, one part flows to the direction of the hole 2222, and is discharged into the waste liquid diversion layer 23 through the hole 2222 and the hole 2322; the other part of the liquid flows to the node The direction of the hole 2224, when reaching the position of the node hole 2224, the liquid will be divided into multiple directions, and flow to the direction of the holes 2214-2221 and the hole 2223 respectively in the plane of the reagent injection guide layer 22, and pass through the holes 2214-2221 and the hole 2223 , holes 2314-2321 and holes 2323 are discharged into the waste liquid diversion layer 23, and part of the liquid will break away from the reagent injection diversion layer 22, and pass through the node hole 2224 and the second node along the direction perpendicular to the reagent injection diversion layer 22. Hole 2324 and hole 15 2524 flow into the reaction pool to achieve the purpose of single reagent entering the liquid without pollution, and all other reagents entering the waste liquid diversion layer 23 are diverted to the first node hole 2312 through the waste liquid diversion layer 23 plane pipeline , through the first junction hole 2312 , the fourth hole 2212 , and the waste liquid outlet 2112 to discharge the system.

Compared with prior art, the beneficial effect that the present invention produces is:

(1) The multi-reagent sequential liquid feeding system in a multi-reagent sequential liquid feeding device for preventing cross-contamination provided by the present invention is a multi-layer structure, that is, at least including a pipe connection layer, a reagent injection diversion layer, and a waste liquid diversion layer , cleaning liquid diversion layer, and reaction pool connection pool, which can realize that the process of multi-reagent liquid feeding and cleaning process is carried out independently, which not only ensures that multiple reagents are fed in sequence without cross-contamination, and avoids the risk of cross-contamination; at the same time It also realizes zero dead volume cleaning of all channels in the system through cleaning liquid;

(2) The multi-reagent sequential liquid feeding system provided by the present invention is a multi-layer structure, including a plurality of independent liquid flow circuits, especially the reagent injection guide layer is provided with a plurality of independent reagent inlets and a waste liquid outlet, which can Quantitative control of reagents is realized, thereby improving the accuracy of reagent injection.

Description of drawings

It further illustrates the technical contents of the present invention, which are described in detail below in conjunction with the embodiments and accompanying drawings, wherein:

Fig. 1 is a structural perspective view of a multi-reagent sequential liquid feeding device provided by the present invention;

Figure 1-1 is a front view of the multi-reagent sequential liquid feeding device provided by the present invention;

Figure 1-2 is a top view of the multi-reagent sequential liquid feeding device provided by the present invention;

Fig. 2 is a three-dimensional schematic view of the pipeline connection layer structure provided by the present invention;

Figure 2-1 is a top view of Figure 2;

Fig. 2-2 is a three-dimensional schematic diagram of the reagent injection guide layer structure provided by the present invention;

Figure 2-3 is a top view of Figure 2-2;

Fig. 2-4 is the three-dimensional schematic view of the structure of the waste liquid diversion layer provided by the present invention;

Figure 2-5 is a top view of Figure 2-4;

Fig. 2-6 is the three-dimensional schematic view of the structure of the cleaning fluid guide layer provided by the present invention;

Figure 2-7 is a top view of Figure 2-6;

Fig. 2-8 is the three-dimensional schematic view of the connection layer structure of the reaction pool provided by the present invention;

Figure 2-9 is a top view of Figure 2-8;

Fig. 3 is a schematic structural diagram of the cleaning process of the cleaning solution in Example 1 provided by the present invention;

Fig. 4 is a schematic structural diagram of the cleaning process of reagent injection in Example 2 provided by the present invention;

Fig. 5 is a schematic structural diagram of the process of injecting the reagents into the reaction pool in Example 3 provided by the present invention;

Fig. 6 is a control schematic diagram of the multi-reagent sequential liquid feeding system provided by the present invention.

Detailed ways

Referring to Fig. 1, Fig. 1 is a schematic structural diagram of a multi-reagent sequential liquid feeding device provided by the present invention, wherein the multi-reagent sequential liquid feeding device includes a housing 1 and a multi-reagent sequential liquid feeding system 2, and a multi-reagent sequential liquid feeding system 2 Set in the casing 1.

Referring to Figure 1-1 and Figure 1-2, Figure 1-1 is a front view of the multi-reagent sequential liquid feeding device provided by the present invention, and Figure 1-2 is a top view of the multi-reagent sequential liquid feeding device provided by the present invention; Among them, the multi-reagent liquid inlet system in the multi-reagent sequential liquid inlet device at least includes a pipeline connection layer 21, a reagent injection diversion layer 22, a waste liquid diversion layer 23, a cleaning liquid diversion layer 24, and a reaction pool connection layer 25. The five layers are distributed sequentially up and down, and may also be distributed in other ways in the present invention.

Referring to Figure 2 and Figure 2-1, the pipeline connection layer includes at least one cleaning solution inlet 2111, multiple reagent inlets 2101-2110, at least two waste liquid outlets 2112, 2113, cleaning solution inlet 2111, and multiple reagent inlets 2101-2110, waste liquid outlets 2112, 2113 can be connected to external infusion pipelines.

As shown in Figure 2-2 and Figure 2-3, the reagent injection guide layer includes a plurality of reagent inflow holes 1 2201-2210, holes 2 2214-2223, holes 3 2211, holes 4 2212, holes 5 2213, and node holes 2224 and diversion pipes connecting different holes; holes 1 2201-2210 are equiangularly distributed around the node hole 2224, and holes 2214-2223 are equiangularly distributed around the node hole 2224, and are formed in the hole 1 2201-2210 outside of the circumference;

Holes two 2214-2223 are respectively connected with node holes 2224 through pipelines, and the pipelines are straight pipelines or arc-shaped pipelines. It communicates with adjacent single pipelines through curved or straight pipelines;

The positions of holes 1 2201-2210 are set corresponding to the positions of reagent inlets 2101-2110 of the pipeline connection layer.

As shown in Figure 2-4 and Figure 2-5, the waste liquid diversion layer is provided with hole six 2311, first node hole 2312, hole eight 2314-2323, second node hole 2324 and guides connecting different holes. Flow pipeline; the eighth holes 2314-2323 are equiangularly distributed around the second node hole 2324, and are set correspondingly to the positions of the second hole 2214-2223 in the reagent injection guide layer; the first node hole 2312, the hole seven 2313 Corresponding to hole 4 2212 and hole 5 2213 respectively; hole 8 2314-2323 communicates with the first node hole 2312 through a straight line guide pipe or an arc guide pipe.

Referring to Figure 2-6 and Figure 2-7, there are hole nine 2401, hole ten 2411, hole eleven 2413 and hole twelve 2424 on the cleaning liquid diversion layer, and hole nine 2401 and hole ten 2411 are in the same layer connected by pipes.

Referring to Figure 2-8 and Figure 2-9, there are holes 13 2501, 15 holes 2524 and 14 holes 2513 on the connection layer of the reaction pool, and holes 13 2501 and 15 holes 2524 pass through the pipeline in the same layer connect.

Embodiment one

Referring to Fig. 1-1, Fig. 2-1, Fig. 2-3, Fig. 2-7, Fig. 2-9 and Fig. 3, Fig. 3 shows the cleaning process of the multi-reagent sequential liquid feeding system provided by the present invention using cleaning liquid Schematic. The multi-reagent sequential liquid inlet system shown in Figure 3 is a multi-layer structure, that is, it includes a pipeline connection layer, a reagent injection diversion layer, a waste liquid diversion layer, a cleaning liquid diversion layer and a reaction tank connection layer, wherein the pipe connection The multi-reagent inlets 2101-2110 in the layer are respectively connected with the reagent inflow holes 1 2201-2210 in the reagent injection diversion layer through the axial pipeline; the cleaning liquid inlet 2111, the reagent injection hole 3 2211 in the diversion layer, and the waste liquid Hole six 2311 in the diversion layer and hole ten 2411 in the cleaning fluid diversion layer are correspondingly connected through axial pipes; waste liquid outlet 2112, reagent injection hole four 2212 in the diversion layer, and the fourth hole in the waste liquid diversion layer A node hole 2312 is correspondingly connected through an axial pipeline; waste liquid outlet 2113, hole five 2213 in the reagent injection diversion layer, hole seven 2313 in the waste liquid diversion layer, hole eleven 2413 in the cleaning liquid diversion layer Correspondingly communicated through the axial pipeline; the second hole 2214-2223 in the reagent injection diversion layer is respectively communicated with the eighth hole 2314-2323 in the waste liquid diversion layer through the axial pipeline; the reagent is injected into the node hole in the diversion layer 2224, the second node hole 2324 in the waste liquid diversion layer, hole 12 2424 in the cleaning liquid diversion layer, and hole 15 2524 in the reaction pool connection layer are correspondingly connected through axial pipes; the cleaning liquid diversion layer Hole nine 2401 in the reaction pool connection layer is connected with hole thirteen 2501 through the axial pipeline; hole fifteen 2524 in the reaction pool connection layer is connected to the inlet of the reaction pool, and hole fourteen 2513 is connected to the outlet of the reaction pool .

Inject the cleaning liquid into the multi-reagent sequential liquid feeding system for cleaning. The purpose of cleaning is to clean all the pipes and reaction pools inside the system with zero dead volume through the cleaning liquid before putting in the reagents, and eliminate all residual reagents or other pollution that may exist in the system. It can be operated before all the reagents enter the liquid or after the last reagent is injected.

The cleaning process is as follows: the cleaning liquid reaches the fifteenth place 2524 through the cleaning liquid inlet 2111, hole three 22211, hole six 2311, hole ten 2411, hole nine 2401, and hole 13 2501. At this time, the liquid is divided into two parts. The reaction pool 26, hole 14 2513, hole 11 2413, hole 7 2313, hole 5 2213 reach the waste liquid outlet 2113 to discharge the system, and another part of the liquid passes through hole 15 2524, hole 12 2424, and the second node hole 2324 Arriving at the node hole 2224, the liquid will be divided into multiple parts on the plane of the diversion layer 22, and will be discharged into the 23rd layer through the second hole 2214-2223 respectively, and then guided to the first node through the 23rd layer plane pipeline Hole 2312, through the first node hole 2312 and hole 4 2212, reaches the waste liquid discharge outlet 2112 to discharge the system; complete the cleaning of many internal pipes and reaction pools, and immediately enter the reagent injection cleaning process after cleaning.

Embodiment two

Referring to Figure 1-1, Figure 2-1, Figure 2-3, Figure 2-5 and Figure 4 shown in Figure 4, Figure 4 is a schematic structural diagram of the multi-reagent sequential liquid feeding system provided by the present invention for the reagent feeding and cleaning process. The principle of the reagent feeding and cleaning process is that the reagents start to be fed, but they do not enter the reaction pool immediately, but cooperate with the discharge system through the cleaning process of the cleaning solution to discharge the reagents that may be contaminated at the reagent inlet. In this embodiment, there are 10 reagent inlets. The process will be described by taking 2109 port as an example to start liquid feeding. Other reagent ports 2101-2108 and 2110 stop liquid feeding, and the cleaning reagent continues the cleaning process. The reagent flows into the channel of the connecting hole 2220 and the node hole 2224 from the hole 2209, and then as the cleaning liquid flows into the waste liquid diversion layer 23, all the reagents entering the waste liquid diversion layer 23 are diverted to the The first node hole 2312 is discharged from the system through the first node hole 2312, the fourth hole 2212, and the waste liquid outlet 2112 to complete the cleaning process of reagent injection. Reagent feeding and cleaning effectively avoids the risk of reagent contamination at the reagent liquid inlet.

Embodiment Three

Referring to Fig. 1-1, Fig. 2-1, Fig. 2-3, Fig. 2-5, Fig. 2-7, Fig. 2-9 and Fig. 5, Fig. 5 shows the multi-reagent sequential liquid feeding system provided by the present invention. Schematic diagram of the reagent injection process into the reaction cell. The process of injecting the reagent into the reaction pool is after the previous process, that is, the reagent is put into the cleaning process, and the injection of the cleaning solution is stopped. The reagent enters the diversion layer 22 through the hole 2109 and the hole 1 2209. On the plane of the diversion layer 22, the reagent flows in from the hole 2209 In the channel connecting the hole 2220 and the junction hole 2224, a part flows to the direction of the second hole 2220, and the other part flows to the direction of the junction hole 2224; the reagent flowing into the direction of the junction hole 2224 will be divided into multiple directions, and a part of it will break away from the flow guide layer 22 , and flow into the reaction pool 26 through the node hole 2224 , the second node hole 2324 , the hole 12 2424 , and the hole 15 2524 along the direction perpendicular to the flow guide layer 22 , so as to achieve the purpose of a single reagent entering the liquid without pollution.

In the process of sequential liquid feeding of multiple reagents, the above three processes can be cycled to achieve the purpose of sequential liquid feeding of multiple reagents without cross-contamination. In the actual process, other operating steps can be added to the circulation process for common circulation. Operation, pre-cleaning and post-cleaning steps can also be added before and after the cycle operation.

Referring to Fig. 6, Fig. 6 is a control schematic diagram of the multi-reagent sequential liquid feeding system provided by the present invention. During the sequential feeding of multiple reagents, all the liquid inlets or outlets in the above three embodiments are opened or closed by the control valve shown in Figure 6, and the control valve is uniformly controlled by the control system.

The above-mentioned series of detailed descriptions are only specific descriptions of the feasible implementation modes of the present invention, and they are not intended to limit the protection scope of the present invention, and are within the knowledge of those of ordinary skill in the art , Various changes made under the premise of not departing from the gist of the present invention all belong to the protection scope of the present invention.

Claims (4)

1. a kind of, to prevent more reagent sequence feeding devices of cross contamination include shell, more reagent sequence feed liquor systems；It is described more Reagent sequence feed liquor system is disposed in the housing；It is characterized in that,

More reagent sequence feed liquor systems are multilayered structure, include at least pipeline articulamentum (21), reagent injects guide layer (22), waste liquid guide layer (23), cleaning solution guide layer (24) and reaction tank articulamentum (25), the pipeline articulamentum (21), examination Agent is injected between guide layer (22), waste liquid guide layer (23), cleaning solution guide layer (24), reaction tank articulamentum (25) adjacent two layers It is to be connected by axial duct；

The pipeline articulamentum (21), reagent injection guide layer (22), waste liquid guide layer (23), cleaning reagent guide layer (24), Reaction tank articulamentum (25) is distributed in sequence up and down；

The pipeline articulamentum (21) include at least one filter washing water inlet (2111), multiple reagent inlets (2101-2110) and At least two waste liquid outlets (2112), (2113)；

The filter washing water inlet (2111), multiple reagent inlets (2101-2110), waste liquid outlet (2112), (2113) are all connected with External infusion pipeline；

The reagent injection guide layer (22) includes multiple reagent ostiums one (2201-2210), hole two (2214-2223), hole Three (2211), Kong Si (2212), hole five (2213), hole six (2311), node hole (2224), and connect the diversion pipe in different holes Road；

The reagent ostium one (2201-2210) is around node hole (2224) in angularly circle distribution；Two (2214- of hole 2223) node hole (2224) are surrounded in angularly circle distribution, and the periphery formed positioned at hole one (2201-2210)；

The hole two (2214-2223) is connected to node hole (2224) by pipeline respectively, and the pipeline is rectilinear duct or arc Shape pipeline；Hole one (2201-2210) is located at the side for the pipeline that hole two (2214-2223) is connected to node hole (2224), And it is connected respectively by arc or rectilinear duct with adjacent single pipeline；

The reagent ostium one (2201-2210) is corresponding by axial passage with reagent inlet (2101-2110) respectively Connection；And the hole one (2201-2210) is opposite with the reagent inlet position (2101-2110) of pipeline articulamentum (21) respectively It answers；

The waste liquid guide layer (23) is equipped with several holes, i.e. hole six (2311), the first node hole (2312), hole seven (2313), hole eight (2314-2323) and the second node hole (2324), and connect the conduction pipe in different holes；

The hole eight (2314-2323) of the waste liquid guide layer (23) around the second node hole (2324) in angularly circle distribution, And, second node hole (2324) and reagent guide layer corresponding with the position (2214-2223) of hole two of reagent guide layer (22) (22) node hole (2224) position is corresponding；Hole six (2311), the first node hole (2312), hole seven (2313) respectively with reagent Hole three (2211), Kong Si (2212), the position in hole five (2213) of guide layer (22) are corresponding；

The hole eight (2314-2323) is connected with the first node hole (2312) by straight line conduction pipe or curve channelization tube road respectively It is logical；

The cleaning solution guide layer (24) is equipped with hole nine (2401), hole ten (2411), Kong Shiyi (2413) and hole 12 (2424), the hole nine (2401) is connect in same layer by pipeline with hole ten (2411),

The reaction tank articulamentum (25) is equipped with Kong Shisan (2501), Kong Shisi (2513) and Kong Shiwu (2524)；The hole 13 (2501) are connect in same layer by pipeline with Kong Shiwu (2524),

The filter washing water inlet (2111), reagent injection guide layer (22) hole three (2211), waste liquid guide layer (23) hole six (2311), the hole ten (2411) of cleaning solution guide layer (24) is corresponded to by axial passage and is connected to；

The waste liquid outlet (2112), reagent inject the first knot of the Kong Si (2212) of guide layer (22), waste liquid guide layer (23) Spot hole (2312) is corresponded to by axial passage and is connected to；

The waste liquid outlet (2113), reagent injection guide layer (22) hole five (2213), waste liquid guide layer (23) hole seven (2313), the Kong Shiyi (2413) of cleaning solution guide layer (24), the Kong Shisi (2513) of reaction tank articulamentum (25) pass through axial direction Channel corresponds to connection；

The hole two (2214-2223) of reagent injection guide layer (22) hole eight with the waste liquid guide layer (23) respectively (2314-2323) is corresponded to by axial passage and is connected to；

It is the node hole (2224) of reagent injection guide layer (22), the second node hole (2324) of waste liquid guide layer (23), clear The hole 12 (2424) of washing lotion guide layer (24), the Kong Shiwu (2524) of reaction tank articulamentum (25) are corresponded to by axial passage to be connected It is logical；

The hole nine (2401) of cleaning solution guide layer (24) passes through axial passage with the Kong Shisan (2501) of reaction tank articulamentum (25) Corresponding connection；The Kong Shiwu (2524) of the reaction tank articulamentum (25) connect with the entrance of reaction tank, Kong Shisi (2513) with The outlet of reaction tank connects.

2. the more reagent sequence feeding devices as described in claim 1 for preventing cross contamination, which is characterized in that the reagent note Enter guide layer (22) and be formed by liquid stream circuit and is：Cleaning solution is injected from filter washing water inlet (2111), cleaning solution is divided into two parts, A part of cleaning solution reaches node hole (2224) through Kong Shiwu (2524), hole 12 (2424), the second node hole (2324), at this time Cleaning solution will flow into reagent from node hole (2224) and inject guide layer, rinse to the channel of each non-fluid injection, not close cleaning In the case of liquid, reagent is injected from any hole in hole one (2201-2210), reagent enters waste liquid with the cleaning solution that stream passes through Guide layer achievees the purpose that cleaning reagent inlet；Be then shut off cleaning solution, at this time reagent ostium two (2214-2223) with It can be divided into two when the pipeline of node hole (2224) connection, a part flows to node hole (2224) direction, a part of negative direction stream It is dynamic；The reagent for flowing to node hole (2224) direction is divided into multiple directions, and a portion is detached from guide layer (22), into reaction Pond, another part branch to each channel, and flow to waste liquid guide layer；Each reagent inlet is finally closed, cleaning solution is opened Pipeline is rinsed, cleaning solution convergence reagent is rinsed to each channel, and flows to waste liquid guide layer.

3. the more reagent sequence feeding devices as described in claim 1 for preventing cross contamination, which is characterized in that the waste liquid is led Fluid layer (23) is formed by liquid stream circuit：The liquid flowed into from the hole eight (2314-2323) of waste liquid guide layer (23) all converges Simultaneously removal system is flow at the first node hole (2312)；Any one hole allows in space structure in hole eight (2314-2323) Under the conditions of can independent diversion to the first node hole (2312), resistance that the liquid to reduce the inflow of other holes is formed.

4. the more reagent sequence feeding devices as described in claim 1 for preventing cross contamination, which is characterized in that the cleaning is molten Liquid guide layer (24) is formed by liquid stream circuit：The hole ten (2411) of cleaning solution guide layer (24) passes through with hole nine (2401) Rectilinear duct or arc pipe connection, will be in the liquid guide flow to hole nine (2401) that flowed into hole ten (2411)；

The reaction tank articulamentum (25) is formed by liquid stream circuit and is：The Kong Shisan (2501) of reaction tank articulamentum (25) and hole 15 (2524) are connected to by pipeline, and Kong Shiwu (2524) is connect with the entrance of reaction tank, the liquid that Kong Shisan (2501) is flowed into Body is branched to via Kong Shiwu (2524) in reaction tank, the pipeline that Kong Shiwu (2524) is formed with hole 12 (2424).