CN106795481B

CN106795481B - Biomarkers for Obesity-Related Diseases

Info

Publication number: CN106795481B
Application number: CN201480082401.4A
Authority: CN
Inventors: 冯强; 张东亚; 唐龙清; 王俊
Original assignee: BGI Shenzhen Co Ltd
Current assignee: BGI Shenzhen Co Ltd
Priority date: 2014-09-30
Filing date: 2014-09-30
Publication date: 2021-05-04
Anticipated expiration: 2034-09-30
Also published as: CN106795481A; WO2016049927A1

Abstract

Biomarkers and methods for predicting the risk of a microorganism-associated disease, particularly obesity or related diseases, are provided.

Description

Biomarkers for obesity related diseases

Cross Reference to Related Applications

Is free of

Technical Field

The present invention relates to biomarkers and methods for predicting the risk of a disease associated with a microorganism, in particular obesity or related diseases.

Background

Obesity is common in developed countries and increases significantly worldwide (de Carvalho Pereira et al, 2013). It has been reported that adults have increased 27.5% and children have increased 47.1% of the prevalence of total overweight and obesity worldwide between 1980 and 2013. Overweight people increased from 8.57 billion in 1980 to 21 billion in 2013, with 6.71 billion population affected by obesity. Among these, over 50% of obese patients live in ten countries, and the united states has the largest number of obese people, followed by china (Ng et al, 2014).

There is increasing evidence that patients diagnosed as overweight by physicians are more likely to lose weight than patients not diagnosed as overweight. However, the low diagnosis rate of physicians is associated with recommendations for health risk factors for obesity-related behavior (Bleich et al, 2011).

In children, the diagnosis of obesity is based on the entry point of the age-and sex-specific Body Mass Index (BMI). This is in contrast to adults, where the diagnosis of obesity is made based on BMI regardless of age or gender. Unlike adults, the diagnostic criteria for obesity are simpler for adults, a few obese children are diagnosed accurately with more complex diagnostic criteria and the terminology for childhood obesity is changed (Walsh et al, 2013). Furthermore, limitations in the identity of BMI among different populations should be considered (Nevill et al, 2006). Thus, Waist Circumference (WC) can be considered a reliable and useful tool for epidemiological studies evaluating abdominal wall adiposity, but such measurements appear to be more difficult to perform (Miguel-Etayo et al, 2014). Furthermore, regional studies on childhood obesity diagnosis using the international classification of disease (ninth revision (ICD-9)), national outpatient medical care survey (NAMCS), and national inpatient medical care survey (NHAMCS) showed relatively low sensitivity of clinical diagnosis (Walsh et al, 2013).

Recent observations indicate that human gut microbiota can play an important role in obesity. Early reports based on amplified 16S rRNA gene sequencing indicated that the ratio of Firmicutes to bacteroides (bacteroides) in stool samples from 12 obese humans was much higher than two lean controls (Ley et al, 2006). Reduced bacterial diversity, relative absence of bacteroides (bacteroides), and enrichment of genes involved in carbohydrate and lipid metabolism have been demonstrated in recent observational studies employing metagenomic sequencing in human obesity (Allin and Pedersen, 2014). These related findings indicate that alterations in gut microbiota are a causative factor in the pathogenesis of obesity. This suggests that perhaps we could use this characteristic of the gut microbiota as a criterion for the diagnosis of obesity.

In summary, there are considerable overlooked opportunities and low sensitivity for the diagnosis of obesity. There is a need to develop more effective (less biased) assessments of overweight and/or obesity.

Disclosure of Invention

Embodiments of the present disclosure seek to address, at least to some extent, at least one of the problems in the prior art.

The present invention is based on the following findings of the present inventors:

the assessment and characterization of gut microbiota has become a major area of research in human diseases including obesity. For analysis of gut microbial composition in obese patients, the present inventors performed a metagenomic association analysis (MGWAS) protocol based on deep shotgun sequencing of gut microbial DNA from 158 individuals (Qin, j. et al, a metagenome-wide association study of gut microbiota in type 2diabetes. nature 490,55-60(2012), incorporated herein by reference). The present inventors identified and validated 396,100 obesity-related gene markers. To exploit the potential capability of obesity classifiers by gut microbiota, the present inventors developed a disease classifier system based on 54 gene markers defined as an optimal gene set by a minimum redundancy-maximum correlation (mRMR) feature selection method. In order to visually assess the risk of obesity diseases based on the 54 intestinal microbial gene markers, the present inventors calculated a health index. The present inventors' data have conducted intensive studies on the characteristics of the gut metagenome associated with obesity risk, providing an example of future studies of the pathophysiological role of gut metagenome in other related diseases and potential applications for the assessment of individuals at risk for such diseases based on gut microbiota.

It is believed that genetic markers of the gut microbiota are valuable for improving the detectability of obesity in the early stages, for the following reasons. First, the markers of the present invention are more specific and more sensitive than conventional markers. Second, stool analysis ensures accuracy, safety, affordability, and patient compliance. And the stool sample is transportable. The present invention therefore relates to a comfortable and non-invasive in vitro method, making it easier for a person to participate in a given screening procedure. Third, the markers of the invention can also be used as therapy monitoring tools for cancer patients to detect their response to therapy.

One aspect of the present disclosure provides a biomarker set for predicting a disease associated with microbiota in a subject, consisting of:

sequence SEQ ID NO: 1 to 54.

According to an embodiment of the present disclosure, the disease is obesity or a related disease.

With these biomarkers, a subject can be analyzed for certain diseases associated with microbiota, e.g., obesity or related diseases can be determined based on certain samples from the subject, e.g., certain stool samples can be used.

In another aspect of the present disclosure, there is provided a kit for determining the above gene marker set, comprising a nucleic acid sequence for PCR amplification and according to the sequence set forth as SEQ ID NO: 1 to 54, or a DNA sequence as described in at least a partial sequence thereof.

Another aspect of the present disclosure provides a kit for determining the above-mentioned gene marker set, comprising one or more sequences according to SEQ ID NO: 1 to 54, and a probe designed from the gene.

Another aspect of the present disclosure provides the use of the above-described gene marker set for predicting the risk of obesity or related diseases in a subject. According to embodiments of the present disclosure, the risk of obesity or related diseases in a subject may be predicted by:

(1) collecting sample j from the subject;

(2) determining the relative abundance of SEQ ID NO: 1 to 54, relative abundance information; and

(3) calculated from I as follows_jIndex of sample j represented:

A_ijis the relative abundance of marker i in sample j, wherein i refers to each gene marker in the set of gene markers;

n is a first subset of the markers enriched in all patients among the selected biomarkers associated with the abnormal condition,

m is a second subset of the markers in the selected biomarkers associated with the abnormal condition enriched in all controls,

| N | and | M | are the number of biomarkers in the first and second subsets respectively,

wherein

An index greater than a threshold value indicates that the subject has an abnormal condition or is at risk of developing an abnormal condition.

According to some embodiments of the disclosure, | N | is 24 and | M | is 30.

According to some embodiments of the disclosure, the critical value is at least 0.5834.

Another aspect of the present disclosure provides the use of the above-described gene marker set in the manufacture of a kit for predicting the risk of obesity or a related disease in a subject. According to embodiments of the present disclosure, the risk of obesity or related diseases in a subject may be predicted by:

(1) collecting sample j from the subject;

(3) calculated from I as follows_jIndex of sample j represented:

wherein

According to some embodiments of the disclosure, | N | is 24 and | M | is 30.

Another aspect of the present disclosure provides a method of diagnosing whether a subject has or is at risk for developing an abnormal condition associated with a microbiota, comprising:

determining the relative abundance of the aforementioned biomarkers in a sample from the subject, an

Determining whether the subject has or is at risk of developing an abnormal condition associated with the microbiota based on the relative abundance.

According to embodiments of the present disclosure, the risk of obesity or related diseases in a subject may be predicted by:

(1) collecting sample j from the subject;

(3) calculated from I as follows_jIndex of sample j represented:

wherein

According to some embodiments of the disclosure, | N | is 24 and | M | is 30.

According to an embodiment of the present disclosure, the abnormal condition associated with microbiota is obesity or a related disease.

Drawings

These and other aspects and advantages of the present disclosure will become apparent and more readily appreciated from the following description, taken in conjunction with the accompanying drawings, wherein:

FIG. 1 correlation analysis of the obesity P-value distribution determines a disproportionate over-representation of strongly related markers at lower P-values.

FIG. 2ROC is plotted by the probability of disease in the training set (training set), AUC 0.9651.

Fig. 3ROC is plotted from the obesity index in the training set, AUC 0.9784.

The ROC for the test set (42 samples) of fig. 4 is plotted from the obesity index for the test set, AUC 0.8729.

The ROC for the test set (22 samples) of fig. 5 is plotted from the obesity index for the test set, AUC 0.9487.

Detailed Description

Examples of the invention

The terms used herein have the meanings commonly understood by those of ordinary skill in the art to which the present invention pertains. Terms such as "a," "an," and "the" are not intended to refer to only a singular entity, but include the general class of terms that may be used to describe a particular example. The terms used herein are used to describe specific embodiments of the invention, but their usage does not limit the invention, unless otherwise specified in the claims.

The invention is further illustrated in the following non-limiting examples. Unless otherwise indicated, parts and percentages are by weight and degrees are in degrees Celsius. It will be apparent to those of ordinary skill in the art that these examples, while representing preferred embodiments of the invention, are given by way of illustration only and that all reagents are commercially available.

EXAMPLE 1 identification of biomarkers for assessing obesity Risk

1.1 sample Collection

Stool samples from 158 chinese subjects, including 78 obese patients and 80 control subjects (training set), were collected in 2012 by the rekins hospital, the shanghai university of transportation medical school. Obese patients are from 18 to 30 years of age with a BMI above 25. Subjects were asked to collect fresh stool samples at the hospital. The collected samples were placed in sterile tubes and immediately stored at-80 ℃ until further analysis.

Full ethical approval was obtained and all patients were given written informed consent. The study was approved by the ethical review committee of the rekins hospital, Shanghai university of medicine.

1.2DNA extraction

Stool samples were thawed on ice and DNA extraction was performed using Qiagen QIAamp DNA pool Mini kit (Qiagen) according to the manufacturer's instructions. The extract was treated with DNase-free RNase to eliminate RNA contamination. The amount of DNA was determined using a NanoDrop spectrophotometer, a Qubit fluorometer (with Quant-iTTMdsDNA BR assay kit) and gel electrophoresis.

1.3 DNA library construction and sequencing of fecal samples

DNA library construction was performed according to the manufacturer's instructions (Illumina, insert size 350bp, read length 100 bp). The inventors performed cluster generation, template hybridization, isothermal amplification, linearization, blocking and denaturation, and hybridization of sequencing primers using the same workflow as previously described. The inventors constructed a paired-end (PE) library with an insert size of 350bp for each sample, followed by high throughput sequencing to obtain about 3 million PE reads of length 2x100 bp. High quality reads are obtained by filtering low quality reads with indeterminate "N" bases, linker contamination, and human DNA contamination from Illumina raw reads and by simultaneously cleaving the low quality terminal bases of the reads.

The inventors output fecal microbiota sequencing data (high quality clean data) of about 5.9Gb per sample in total from 158 samples (78 cases and 80 controls) on the Illumina HiSeq 2000 platform (table 1).

Table 1 metagenomic data summary. The fourth column reports the results from the Wilcoxon rank sum test.

1.4 metagenomic data processing and analysis

1.4.1 read alignment

The present inventors used a newer human intestinal gene catalog established by Li, J.et al, An integrated catalog of reference genes in the human gut genome. Nat.Biotechnol. (2014) (incorporated herein by reference) and aligned high quality reads to the newer human intestinal gene catalog with An alignment standard identity ≧ 90. The average read alignments are shown in table 1. This alignment is close to the sample in Li, j, et al, 2014, supra, indicating that this alignment is sufficient for further studies. After read alignment, the inventors derived a gene profile (9.9Mb gene) from the alignment results using the same method as Li, j. et al, 2014, supra.

Taxonomic assignment of genes. The internally developed protocol (pipeline) described in published papers (Li, j. et al, 2014, supra) was used for taxonomic assignment of predicted genes.

1.4.2 data File construction

Gene profiling. Based on the results of the read alignment, the inventors calculated relative gene abundance using the same method described in the published T2D paper (Qin et al, 2012, supra).

1.4.3 analysis of factors affecting the genetic profile of gut microbiota. Based on the gene profile, the inventors used non-parametric multivariate analysis of variance (PERMANOVA) to assess the impact of 6 clinical parameters, including age, gender, height, weight, BMI, and obesity. The inventors performed the analysis using the method implemented in the "vegan" package in R, and obtained the substituted (permuted) p values by 10,000 substitutions (permutation). The inventors also used the Benjamini-Hochberg method to correct multiple tests with "p.adjust" in R to obtain the q value for each test. PERMANOA identified three important factors (based on gene profiling) associated with gut microbiota (q <0.05, Table 2). Analysis showed that body weight, BMI and obesity status are strongly correlated markers, demonstrating that disease (obesity) status is a major determinant affecting the composition of the gut microbiota.

Table 2 PERMANOVA based on Euclidean distance analysis of gene profiles. Analyses were performed at q-values <0.05 to test whether clinical parameters and obesity status have significant effects on gut microbiota.

Phenotype	Df	Sqs summary	Average Sqs	F. Model (model)	R²	Pr(>F)
							Age (age)	1	0.317034738	0.317034738	1.004112579	0.006395454	0.4094
Sex	1	0.377329497	0.377329497	1.196542903	0.007611763	0.1727
							Height of a person	1	0.331409667	0.331409667	1.049947284	0.006685435	0.3291
Body weight	1	0.969536515	0.969536515	3.111941857	0.019558192	1.00E-04
							BMI
	1	0.954186893	0.954186893	3.0617069	0.019248548	1.00E-04
							Obesity
	1	0.972185352	0.972185352	3.120613959	0.019611626	2.00E-04

1.4.4 determination of obesity-related markers

Determination of obesity-related genes. To determine the association between metagenomic profile and obesity, a two-tailed Wilcoxon rank-sum test was employed in the 9,879,897 high frequency gene (removing genes present in less than 10 samples in all 158 samples) profile. 396,100 gene markers enriched in both cases and controls were obtained with p values <0.01 and FDR 3.8% (FIG. 1).

False discovery rate estimation (FDR). The inventors applied the "q-value" method proposed in the previous study to estimate FDR (storage, JDA direct approach to false discovery rates. journal of the Royal Statistical Society 64,479-498(2002), incorporated herein by reference), rather than the sequential p-value elimination method.

Receiver Operating Characteristic (ROC) analysis. The inventors applied ROC analysis to evaluate the performance of the metagenomic marker-based obesity classification. The inventors then used the "pROC" package in R to plot ROC curves.

1.4.5 construction of MLG and identification of MLG species markers associated with obesity

237 MLG species based on the gene profile of markers associated with 396,100 obesity. The present inventors constructed Metagenomic Linkage Groups (MLGs) using 396,100 gene markers using the same method described in the published T2D paper (Qin et al, 2012, supra). All 396,100 genes were annotated by aligning them to 4,653 reference genomes in IMG v 400. MLG is assigned to the genome if more than 50% of the constituent genes are annotated to the genome, otherwise it is referred to as unclassified. A total of 237 MLG genomes with a gene factor >100 were selected (P value < 0.01). To estimate the relative abundance of MLG species, the inventors estimated the average abundance of genes of MLG species after removing the lowest of 5% and the highest abundance of 5% (Qin et al 2012, supra).

1.5 MLG-based classifier

Random forest models (R.2.14, random forest 4.6-7 packages) (Liaw, Andy & Wiener, Matthew. Classification and Regression by random forest, R News (2002), Vol. 2/3, page 18, incorporated herein by reference) were trained using the MLG abundance spectra of training cohorts (158 samples) to select the best set of MLG markers. The model is tested on more than one test set and the prediction error is calculated.

For the random forest model, using "random forest 4.6-7 packets" in R version 2.14, the training data set (i.e., the relative abundance spectrum of MLG in the selected training sample), the sample disease state (the sample disease state of the training sample is the vector, 1 is obesity, 0 is the control), and the test set (only the relative abundance spectrum of MLG in the selected test set) are entered. The inventors then constructed a classification using random forest functions from random forest packets in the R software, and predicted the test set using a prediction function. The output is the prediction (probability of disease; cut off value 0.5, if the probability of disease ≧ 0.5, then the subject is at risk for obesity).

54 MLG species markers were identified. To identify 237 MLG species markers, the inventors adopted "random forest 4.6-7 packages" in R version 2.14 based on 237 obesity-related MLG species. First, the inventors ranked all 237 MLG species by the importance given by the "random forest" method (Liaw, Andy & Wiener, Matthew, Classification and Regression by random forest, R News (2002), volume 2/3, page 18, incorporated herein by reference). The MLG marker set was constructed by creating incremental subsets starting from 1 MLG species and ending to the top-ranked MLG species containing all 237 MLG species. For each MLG marker set, the inventors calculated the false prediction ratio over 158 samples. Finally, the 54 MLG species sets with the lowest false prediction ratios were selected as MLG species markers (table 3-1). Furthermore, the inventors plotted an ROC curve based on the selected MLG species markers (table 3-2) using oob (out of bag) from random forest model to predict disease probability, and the area under the ROC curve (AUC) was 0.9651 (fig. 2) in 158 samples. At the optimal cut-off value of 0.5294, the True Positive Rate (TPR) was 0.8625 and the False Positive Rate (FPR) was 0.07692, indicating that these 54 MLG markers can be used to accurately classify obese individuals.

TABLE 3-154 most discriminatory MLGs (species markers) associated with obesity

TABLE 3-2158 samples predicted results for 54 MLGs

1.6 method of selecting 54 best markers from among biomarkers (maximum associated minimum redundancy (mRMR) feature selection framework)

To determine the optimal gene set, a minimum redundancy-maximum correlation (mRMR) (for details see Peng, H., Long, F. & Ding, C. feature selection based on organizational information: criteria of max-complexity and min-redundancy, IEEE Trans Pattern animal Mach Intel 27,1226-1238, doi: 10.1109/TPAMI.2005.159(2005), incorporated herein by reference) feature selection was used to select from the 54 MLG markers. The inventors selected one gene to represent MLG using 54 MLG markers. The present inventors selected representative genes in each MLG by the mRMR method. And the inventors selected the first gene to represent the MLG. Thus, the inventors obtained 54 gene markers, which are shown in tables 4 and 5. Gene id is from a reference gene list that has been published as Li, j, et al, 2014, supra.

TABLE 4.54 enrichment information for the best Gene markers

TABLE 5.54 SEQ ID of the best Gene markers

1.7 intestinal health index (obesity index)

To develop the potential for disease classification by gut microbiota, the inventors developed a disease classification system based on the 54 gene markers defined by the inventors. In order to intuitively evaluate the risk of disease based on these intestinal microbial gene markers, the present inventors calculated an intestinal health index (obesity index).

To evaluate the effect of gut metagenome on obesity, the inventors defined and calculated an gut health index for each individual based on the 54 gene markers selected as described above. For each individual sample, the formula I is calculated_{j represents}The intestinal health index of sample j of (a):

A_ijis the relative abundance of marker i in sample j;

n is the subset of markers enriched in all patients among the selected biomarkers associated with the abnormal condition (i.e., the subset of markers enriched in all obesity among the 54 selected gene markers),

m is the subset of markers enriched in all controls among the selected biomarkers associated with the abnormal condition (i.e., the subset of markers enriched in all controls among the 54 selected gene markers),

| N | and | M | are the number of biomarkers in the two subsets, where | N | is 24 and | M | is 30,

an index greater than a threshold indicates that the subject has obesity or is at risk of developing obesity.

1.8 Classification of obesity based on the gut microbiota

The inventors calculated an obesity index based on the relative abundance of these 54 gene markers, which clearly distinguished the microbiome of obese patients from the control microbiome (table 6). The 78 obese patient microbiome was classified from the 80 control microbiome using the obesity index, which showed an area 0.9784 under the Receiver Operating Characteristic (ROC) curve (fig. 3). At the optimal index cut-off value of 0.5834, the True Positive Rate (TPR) was 0.9103, the False Positive Rate (FPR) was 0.075, and the error rate was 8.86% (14/158), indicating that 54 gene markers could be used to accurately classify obese individuals.

TABLE 6 calculated intestinal health index for 158 samples (obese patients and non-obese controls)

Example 2 validation of 54 Gene biomarkers in 42 samples (test set)

The present inventors validated the discrimination ability of the obesity classifier using another new independent study group, including 17 obese patients and 25 non-obese controls collected at rekins hospital, the Shanghai university of medicine.

DNA from each sample was extracted and a DNA library was constructed, followed by high throughput sequencing as described in example 1. The inventors calculated the gene abundance profiles of these samples using the same method as described in Qin et al, 2012, supra. Then determining the sequence as shown in SEQ ID NO: 1-54, relative abundance of the genes for each marker. The index for each sample was then calculated by the following formula:

A_ijis the relative abundance of marker i in sample j;

n is the subset of markers enriched in all patients among the selected biomarkers associated with the abnormal condition (i.e., the subset of markers enriched in all obesity among these 54 selected gene markers),

m is the subset of markers enriched in all controls among the selected biomarkers associated with the abnormal condition (i.e., the subset of markers enriched in all controls among these 54 selected gene markers),

wherein an index greater than a threshold value indicates that the subject has or is at risk of developing obesity.

Table 7 shows the calculated index for each sample and table 8 shows the relative abundance of the relevant genes for representative sample DB 68A. In this assessment analysis, at a cut-off of 0.5834 (the best index cut-off in the above 158 samples), the error rate was 26.19% (11/42), demonstrating that 54 gene markers could be classified as obese individuals. And most obese patients (13/17) were correctly diagnosed as obese. In addition, the ROC for the test set was plotted from the obesity index for the test set, AUC 0.8729 (fig. 4). At the optimum threshold 0.7769, the True Positive Rate (TPR) was 0.7647 and the False Positive Rate (FPR) was 0.04.

TABLE 7 calculation of the gut health index for 42 samples

TABLE 8 relative abundance of genes in sample DB68A

Example 3 validation of 54 Gene biomarkers in 22 samples (test set)

The inventors verified the discrimination ability of the obesity classifier using another 22 samples (table 9) including 9 case samples and 13 control samples (5 samples after 1 month of operation and 8 samples after 3 months of operation), which were also collected at rekins hospital, the medical institute of Shanghai transportation university. Cases represent pre-operative samples and controls represent 1 month and 3 months post-operative.

TABLE 9.22 information on samples

Before: before operation; 1-M: surgery after one month; 3-M: three months later.

A_ijis the relative abundance of marker i in sample j.

Table 10 shows the calculated index for each sample and table 11 shows the relative abundance of the relevant genes for representative sample DB 62. In this assessment analysis, the error rate was 18.18% at a cutoff value of 0.5834 (the best index cutoff value among the above 158 samples) (4/22), confirming that the 54 gene markers could be classified as obese individuals. And most obese patients (7/9) were correctly diagnosed as obese. In addition, the ROC for the test set was plotted from the obesity index for the test set, AUC 0.9487 (fig. 5). At the optimum threshold 0.02538, the True Positive Rate (TPR) is 1 and the False Positive Rate (FPR) is 0.1538.

TABLE 10 calculation of the gut health index for 22 samples

Sample (DB: obesity)	Index of obesity
		DB62	1.191905591
DB67	0.025381992
		DB68	1.757974404
DB78	1.344989391
		DB85	1.796053682
DB124	0.072164965
		DB125	1.162137206
DB126	0.979123077
		DB01	0.686585017
DB.S1.62	0.879906331
		DB.S1.68	-0.274438487
DB.S1.85	0.0154326
		DB.S1.124	-0.750440603
DB.S1.125	-0.893868407
		DB.S3.62	0.711881869
DB.S3.67	-0.007230148
		DB.S3.68	-0.029903064
DB.S3.78	-0.761996663
		DB.S3.124	-0.588485485
DB.S3.125	-0.575369569
		DB.S3.126	-0.398672766
DB.S3.01	-0.420476048

TABLE 11 relative abundance of genes in sample DB62

Thus, the inventors identified and validated 54 marker sets by a minimum redundancy-maximum correlation (mRMR) feature selection method based on 54 obesity-associated gut microorganisms. And the inventors established an intestinal health index, and evaluated the risk of obesity based on these 54 intestinal microbial gene markers.

Although illustrative embodiments have been shown and described, it will be understood by those skilled in the art that the above embodiments are not to be construed as limiting the present disclosure and that changes, substitutions and alterations can be made thereto without departing from the spirit, principles and scope of the present invention.

Sequence listing

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acggtgcagg tgctgagcta cctgctggcg atgaagcaga gcggccagac tgagagcaaa 2100

gggctgctgc ccagcctcat cgtctgcccg gcctcgctgg tgctgaactg ggccgaggag 2160

tgccggaagt tcacgccgga gctgatctgc gtggtggtgg acggcgacgc aacccaccgg 2220

gctgcgctgg ctgagagctg gcccgccgcc gacctcgtgg tgacgagcta cgacctgctg 2280

cgccgggatg aggctcttta cgaggggcag gagttctacg cctgcatcct cgatgaggcg 2340

caggccatca agaaccacac cacccagaaa tataaggccg tctgcaaggt gcgcagccgg 2400

gtacggttcg ccctcaccgg cacgccggtg gaaaaccggc ttggcgagct ttggagcatc 2460

ttttccttcc tgatgccggg ctatctgccg ccctataaaa gcttttgcag ccggttcgag 2520

aagcccatcg tgcaggaaga ggaccagact gccgtccggc ggctcaacca gctgaccggc 2580

cccttcatcc tccgccggat gaaagcggat gtgctgaagg agctgccccc taagaccgag 2640

aacgtctacc gcatcgagct ggaagaagag cagcggaagc tctatctggc tgcggtggtg 2700

gacgctaggg agaagctgcg cgccgcaaag ccggaggaca agatggcggt gtttgctgtc 2760

ctcatgcggc tgcgggagat ctgctgcgac ccgcgcctta tcgcggacaa ctgggagggc 2820

ggcagcgcca agctggatgc ttgcgtggag ctgatctccg gcgccgtgga gggcggccac 2880

cgcatcctgc tgttcagcca gttcacctcc atgctggagc tgctggcaaa gcgtctggat 2940

gccgagggca tcagccactt taccctccaa ggctcgacgc cgaaaccggt ccgggcggag 3000

cttgtccgga ggttcaacgg cggcgaggtg agcgtctttc tcatctcgct gcgggcgggc 3060

ggcacgggcc tcaacctgac ggcggcggac atcgtcatcc actacgaccc gtggtggaat 3120

gtggccgcgc agaatcaggc caccgaccgc gcctaccgca tcggacagca gaacccggtg 3180

caggtgtata agctcattgc acaggacacc attgaagaaa agatcgtgga attgcagcag 3240

gccaagcagt cgctggccga caccgtgacc ggcaccgccg atggtgccat cctctcgatg 3300

cggccggacg aactgctgca gctgctggaa gggagcgaag gatga 3345

<210> 2

<211> 504

<212> DNA

<213> Coprococcus comes

<220>

<223> Coprococcus coms isolated from intestinal tract

<400> 2

gtgtttataa taataaaagt agtcctcgat agctcagcgg tagagcattc ggctgttaac 60

cgaagggtcg ttggttcgaa cccaactcgg ggagttctaa aatctgtcag ccaattactt 120

gcagatttta attttgaaaa aatcaatatc cggctccgtg gtcaagcggt taagacatcg 180

ccctttcacg gcggtaacac gggttcgatt cccgtcggag tcatctggcg ccatagccaa 240

gtggtaaggc aaaggtctgc aacacctcta tcaccagttc aaatctggtt ggcgcctcta 300

ggagaaatct ggaaacaatt gtttccagat tttttggtat acaaatataa tataccatat 360

ataaaaatgc ggtatgtgaa gaatgtgctt cctacatcaa aaaataacag aaaatatatt 420

gaaaatcagc tttcgggagc cattgagcag atggaagaat atatcagaaa gtatccgatg 480

tttcttgtta tggattatgt gtaa 504

<210> 3

<211> 186

<212> DNA

<213> Dorea longicatena

<220>

<223> Dorea longicatena isolated from intestinal tract

<400> 3

atgagaaatc ccccttttca taaaataaat cttgttctcc gaattataat atatgtcgct 60

tttgtgcttc aaattgtaaa ttgtacttat aaaaaaacga acttttttaa ttatacttat 120

aaaaaggggt ggaaaaataa gaaggtttta tatataatag ggatagaaac gttcaaaacc 180

acatag 186

<210> 4

<211> 3258

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 4

atggagaaaa accggggact gcggcggcag gtgctgctgg tgccggagca tatttcccat 60

gaggcggagg tggacctgtg ccgggccctg gggcccacgg cgtcccggga tgcggaggtg 120

ctgagcttcc ggagtctgtc gtcccgggtg ctggccgaaa cgggagggct ggcggagttt 180

accctggaca acgggggaaa gctcctgacc atgcggcggg ttttgcagga gctggcgccg 240

gagctgcggg tgttcgggcg gcccagtcag cgggcgtcgt ttctgcggca gctgacggac 300

ctgatggagg agctttacgc ctatgagatc gggccccggg acctctatgc acatgtagag 360

gacctggagg gcgctatggg cgataagctg cgggatatcg cgctactgta tgcggcgtat 420

gacgcccggc tgcgcaacgg agacatggat gcccggtccc ggctccagaa gctccgggag 480

catctggagc acagcgacta tctccggggc tgcgatgtgt atctggacgg gttttcctat 540

ttcaaccgcc aggaggagag catattggag ctggtgctgc ggcaggccag cagcgttacc 600

gtgaccctgc tgggggaccg gggcaatgaa cagctgtttc agaatgctct gcgccagcgg 660

atgcgcctgg agcgcatggc ccggcgtgtg gggcagagta tggaagtcct gtggtgcgaa 720

aagcagggga gcggccccct ggatcacctg gagcggcact tttttggccg ggagacggcc 780

tatgagggcc aggcggaggg aattcgcctg tatcaggccg ccacggcctt caccgaggtg 840

gaatgggtgt cccagcagct gaggcagctg gcggctgagg gataccgctg gcgggatatg 900

ggcgtgtgcg cccggaatat ggaggtttat ggcccactgc tggagagcgt gttccgccgg 960

gacggcatcc cggcctatat cagccgccgc agtgatctgc tggaaaagcc gcccatcacc 1020

atgcttttgg gggccctgga cgccgttacc ggcggctttg accgggagga cgtgttccgc 1080

tgcctgaaga ccggcctggg gggcatttcc ccggatgagt gcgatatcct ggagaattat 1140

gtgatcgtgt gggatatccg gggcggcatg tggctgcggg agagcgactg gaccgccagc 1200

cccgacggct acggacggga gatgacggag actgccgccg cacggctgcg gcaggtcaat 1260

ggcgtccggc ggcgggtacg tctgctgctg gagcccctgt atcaggggat gaaaacggca 1320

tcatccgcca ggagcaaggc ggagtgcctg tacacatttt tgcaggaggc cggtgtgccc 1380

caggccctgg aggaaaccgc cgggaagctg ctggcagaga accagcccca gctggcggag 1440

gaatatgtgc agctgtggga gatattctgc ggggtgctgg accaatttgt ggagatcctg 1500

ggggacacgg agctgacggg tgaggagttt gcgcggctgc tgcggctggt gctgacccag 1560

tacagcgtgg gtaccattcc tgccaccctg gaccaggtga aggtcagcga gatcacccgc 1620

aacgaccggc accgggtaag gtgcctgttc ctgctggggg ccaacgacca cgtgctgcct 1680

atgccgccca gcggccacgg cctgctggac ccggaggacc gggagacctt gcagcagcgg 1740

gagatcctgc tgtcggacgc cgtgtttgac ccgctggaca acgagctgca gaatatttac 1800

gcgtgcctgg cccagcctac ggagcggctt accgtcagct ggccggtggg ggacatcagc 1860

ggcgctcagc tgcggccctc cttcgtggtg cagcggatgg agaagctgtt tccggacctg 1920

cacgtcctgc gggaggacgg ggcctaccgg cggatgctgc ctgccacggc tctggagctg 1980

gccggcagcg acagggcact ggagcgatat ttccggcagc ggggcggcta tgaccgggtg 2040

ctggatgcca tggaccgggg ccgcgccctg ggccgggggc ggctgtcgcc ggaggcggtg 2100

caggccctgt atgggcggtc cctgcacatg tcggcctccc ggatggacca ggtgaagcgg 2160

tgccacttcg ggtacttcat gcagtatggc ctgcgggcca gagagcgccg accggcgggc 2220

ttcgaggccc cggagatcgg cacgttcatc cactatttat tggaaaatgt ggcccgggag 2280

gtaaagaacc ggggcggctg gagccaggtg gagcgggagg agctgcgccg cctggtccgg 2340

gaatacatag accaatacgc ccgggaggag atcgacggct atcgggagaa aagcgcccgg 2400

ttccggtatc tgttttcccg gctgcggacg gcggcttgcg ccatcgtgga ggacatggcc 2460

ggggagctgg cccaatcgga tttttcaccg gtggccttcg agctgggctt tggcggccgg 2520

gacgggcagc tgcccgccgt taccgtcacc gagggagatg agaccctgtc cgtcagcggc 2580

aaggtggacc gggtggacgg ctggctccac gacggaaagc tgtatctgcg ggtggtggac 2640

tacaaaaccg ggaaaaaagc ctttgacctg tcggacctgc ggtacggcct gggtattcag 2700

atgctcctgt acctgttcac tctggagaag gagggccgca gctatttcgg ctatcccatt 2760

gtgccggcgg gggtactgta tcacccggcc cgggaggtga tcctgaaaaa ggaccggggc 2820

atccagccgg aaaagctcca ggctgccctg caggaggagc tgcgccgcag cggcatggtg 2880

ctggcggacc cggaggtgct gcgggctatg gagcacagcg ctctggaagc accccattat 2940

ctgcccattc gggtgaagaa ggacggctcc atttccgacg gcatcgcatc ggcggagcag 3000

ctggggaagc tgggccggta tgtggaggac ctgctgcacc agatcgccag agagatcggc 3060

tccggcaata ttgacgccga cccggtggca cggggacccc aggagcgggc ctgcgaccgc 3120

tgcgattttg ctgcggcctg tgccttccag gagggccggg gcggcgatcg ggtgcgctat 3180

atcagaactg taaagccgga ggaattctgg caattcgtgg acgcggaaaa tgggaaggag 3240

gggagcacat gccgctga 3258

<210> 5

<211> 825

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 5

atgtccttga agcttgtcat ttatcacata tctgtgaatt attttttagg gggaatattt 60

atggacgtag atatcattct ggatgccggt cacggcggtt ttgacaacgg tgcatcctac 120

atgggacgcg cagaaaaaga tgatgtactc cggctgacac tggctgtcgg agcaaagcta 180

aaagaagcag gttatcatgt ctactacact cgaactacgg atatctataa ctcaccaacg 240

gaaaaagcag cccttgccaa tctctccggt ggaaaatatt ttatttcctt tcaccggaat 300

tccggcatgg aagacaatct gtacaacggt gtgcagaccc ttgtctatca gcatgatcca 360

acggttgata cgctggctga aaatattaac cggcacttgg agcaggtcgg tttcaaaaat 420

ctcggtgtgg aggaagttcc gggactgatc gtccttcgaa aaacagaaat gcccgcagta 480

ctggtagaaa ccggattctt aaacagcgac attgacaatc agctctttga tgaaaaattt 540

gatgccattg ttgatgctat tgtcgccggt atcgaggaaa gcattccact ctccgcccag 600

gaaatcccgg aacaatatta tgtacaaacc ggcctgttca aatatgacgt caatgctgcc 660

tatcagctcg aacgtctgca aattcttggt ttcgacggcc ccatccacta cgaagaacct 720

tactacggcg tctggatcgg caaaccgaaa gacctagatg aagcagttct cctgcaggat 780

gaacttcgcc agaaaggata cagcaccctg atcgtgtcgg tataa 825

<210> 6

<211> 1665

<212> DNA

<213> Haemophilus parainfluenzae

<220>

<223> Haemophilus parainfluenzae isolated from intestinal tract

<400> 6

atgaaaaaat tactttgctc attatttgcg ctttctgctg tgagtacagc aatggcacaa 60

gaagtgaata ttaaattatt aggtacttca gacattcacg gtcgcgttgt gccttggagc 120

tatggcgcag acgtagaaga taaatctggt tcttatgcgc aaatcgcaac ttatgtgaaa 180

gatgtgcgta aaaacaataa aaacgtggtg ttagtggatg tgggcgatgc gatccaagat 240

aaccaagttg atgtgttcgc aaaagataaa aaatattaca aagatcaccc aattccaaaa 300

gtattaaacg aaatgaaata cgatgttttc gtacttggta accacgagtt taactttggg 360

atggaagcat tagatgaaat cttaaaagac atcaaagcga aaaaattaac cgcaaacttc 420

tattataaga aaaatgacaa acgttatatt gatgcaacaa ccatcattga aaaagatggc 480

gtaaaactag ggattatcgg tttaagtacc ccaatgtcag caaaatttga agaagacacg 540

ggcaacttaa aagacatgaa atttacttca ccaacagaag aagcgcgtac acaagttgag 600

aagttaaaag caaaaggtgt tgatgcgatt attgtgatcg ctcacatggg catcgagaat 660

gaaaacaaga ttcctgacac aggtatgcgt gatgtgatca atgcagtaga cggcattgat 720

gtagttatcg cgggtcacat gcacaaagat gtaccaagtg aaaccattaa aaatacacta 780

atcaccgaac cacaccgtta cggtaccgta gtatctgaag tggatctcac ttttgatatc 840

aacgataaaa aagaagtgaa attggtgaag aaagaatcca aaaccgttcc agttaaagaa 900

cttgaagcgg ataagaaaat tgccgaaatc tacaaacctt atcacgagaa attacgtgaa 960

ttaaacaatg tagtgattgg ccaaaccgaa aacgaaatgg tgccacaaga aactaaacat 1020

ggtgtatctg ctgcattctc aaaagatacc ggtttgtctt cattcattaa tgatgttgaa 1080

caacattaca gcggtgcaga tgtggtaact ttctctttcg atcaccaaaa agcacgtatg 1140

aataaaggcg atatcaagaa aaaagacatc atctttaact atcgttatgc gggcggtgat 1200

gtcaccgttt atgaactgac cggtaaacaa ttgaaagaat atatggaatg gtcagcgaac 1260

tactttgata ctattcaacc aggtgacact gaataccgct acaatgcaga acgtaaaaaa 1320

tcaaaatatg tgacttacga cattttcggt ggcgtaaatt acaaaattga cttacgtaac 1380

ccacaaggta gcaaaatcgt tgatttaacc cttgctgacg gcaaaccggt aactgatgac 1440

atgaaattaa aagtgggtat gaactcatat cgtttcgctc agttaaacgg taaaggtggt 1500

atttgggaag gccaacaaat tccggtactt tgggaatcta aagtcgcaat gggccgtgaa 1560

aaaggcacga tccaaaatat gatgatcgat tacatcacca atgtgaaaaa aggcaaaatc 1620

gatggtcaat ctcacaaccg ttgggaaatc attggattaa attaa 1665

<210> 7

<211> 546

<212> DNA

<213> Dorea longicatena

<220>

<223> Dorea longicatena isolated from intestinal tract

<400> 7

agaagctggg ttgaatttct agaaggagga gcacacatga gacaggaacg tattgatgct 60

aattcattag aattaaacga aaaagtagta tcaattaaac gtgtaaccaa agttgttaag 120

ggtggccgta atatgagatt cacagcttta gtagttgttg gtgacggaaa tggccatgtt 180

ggtgcaggtt taggaaaagc tacagaaatt ccggaagcaa tccgcaaagg aaaagaagat 240

gcagctaaga atctcatctc tgtagcatta gatgaaaatg acagtgtaac acatgattat 300

atcggtaaat tcggaggcgc ttctgtatta cttaagaagg ctccagaagg tactggagtt 360

atcgccggtg gtccggcgcg tgccgtaatc gagatggcag gaatcaagaa cattcgtaca 420

aaatctcttg gttctaacaa caaacagaat gtagtacttg ctacaatcga aggattacgc 480

cagattaaaa ctccagaaga agtagctaag cttcgcggta aatctgttga agagatcttt 540

ggctaa 546

<210> 8

<211> 1455

<212> DNA

<213> Alistipes shahii

<220>

<223> Alistipes shahii isolated from intestinal tract

<400> 8

atgaaaaaat taaccgatat tctgaaaaat acccccgtcc gggaggtgtg cggcgatact 60

gccgccgcca ttggcgggct ggtctacgat tcgcgcgccg tgaaaccggg cgactgcttc 120

ttcgccgttc cgggcacgca gagcgacgga cacgactata tccccatagc ggttgaaaaa 180

ggcgcggcgg caatcgtctg cgaacggatg ccggcggatg cggcggaggg cgtcgcttat 240

gttctcgtcg gggactcggc gggggccatg gccgatatgg ccgcggcgtt ctacgactac 300

ccgagccgcg agctgaaact cgtgggcatc accgggacca acggcaagac cacgaccgcg 360

acgctgctct acgatctggt gcgggccatg ggctacaagg ccgggctgat ctcgacggtg 420

gtctacaaga tcgacggccg cgaggtcgaa gccacccaca ccacgcccga ttcgatccgc 480

ctgaacgcta tgatgcgtga aatggccgac gccgggtgcg catactgttt catggagtgt 540

tcgtcgcacg ccatcgtgca ggaacgcacg cggggcctcg atttcgcggg tggcatcttt 600

tcgaacatca cgcacgacca cctcgactac cacaagacct tcgccgagta catccgggcc 660

aagaagctct ttttcgacgg gctgcccaag ggggctttcg ctctgacgaa cgccgacgac 720

cggaacggac gcgtgatggt gcagaacacc gcggcggcgg tgagcgccta ttcgctgcgc 780

gcgatggccg atttccgctg caagatcgtc gagatgcacc tcgacggcat gctcctgcgc 840

atcgacgggc aggagttgtg ggtggggttg ctggggcggt tcaacgccta caacctgctg 900

gcggtctacg gcgcggcggt gttgctggga ctcgaccggg gcgaggtgct gcgcgtgctg 960

agcatgctgc acgccgtgag cggccgcttc gagaagatcc gcgcggcgaa cggcacgacg 1020

gctatcgtcg actacgcgca tacgcccgat gcgctggaga acgtgattca gaccatcgag 1080

gagatccgca cccccggcca gcagctgatc gtggtgtgcg gctgcggcgg cgaccgcgac 1140

cggaccaagc gtcccgagat ggcggccatt gccgtgaagt atgccacgac ggcggttttc 1200

acctccgaca atccgcgcca cgagtcgccc gaggcgattc tcgacgacat ggtcgcgggg 1260

ctggagcccg gtgcgcgcta cctgcgcatc gccgaccgcg ccgaggcgat ccgcacggcc 1320

gtgatgctct cgcgtccggg cgacatcatc ctcgtggcgg gcaaggggca cgaaacctat 1380

cagattgtcg gcgacgtgaa acatcctttc gacgaccggg aagagataaa gaagcgcttt 1440

gcgcttctca attaa 1455

<210> 9

<211> 8007

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 9

atgaatcgtt ttgataatga aagtcggcgg actgcggaag cgcaaaaagg aaacggcggt 60

gaactcggcg cagcggcgcg tgagctgagc aaaatcgcgg ttgaggaaac caaaagaaca 120

acaggcttat cgcagagggc ggcatacaga aagcgtctgt ttgaaatgcg gcgcgggttg 180

aacggggaat atcgcaggaa ttatgcactt gccgccggca gcgttttttg cggtgcggtc 240

ggagaagtgc tcgtgaacga atactatcgt gttgaaaaac agcttcggat tgccgcagcg 300

gaagcggaaa gtttgaaatt cggacggctg ccgtgctttg cggcgggcga ggcggcgggc 360

agccttcgct gcgcagtgct tgcaaagaaa ctctgcgagc tttgcggcgg tgcaccgggc 420

attggctccg tcgttgagtt tttcgatgag taccagcaaa acagacccct gacaacgcgt 480

gaaatacagc tgcttcccgc gatgcttcgg cgggcggagc ttgagacttt gtacggcatt 540

gtatgcacgt caggtgatgg cccgctcgga accgggcgcg cggcagctct gcagaacgtg 600

cttgccgcat tatcaagaat tgacgatgcg gatttttcgg agtgcattca aaacctgaac 660

ataacccgcc gcaggctggc ggatgcagcg gatttttcgg cttccgatga gtcaacgcag 720

cagctttatc ttaaagcggc ggccgaaatt tcggaacgga cgtcggcgtc ggaacagacc 780

gtggttcgga aggcgttcga actatcaaag gggcggacgg ggcgccgcgc tcttgtgggc 840

tgcagtctgc ttgcggaggg gaagccggag ctgcttcgtg cgcttcgtgc ggagaaaaaa 900

cgattaaaag gattaaaaaa cgacaacggc gcaaaggctc cctccgattc gggaaaaaga 960

accgatgtcc aaaaacataa atacgtaaaa ttatcaaatt cgacaaaatg cgcactgctt 1020

acggtttttg aagcattgct tgcggctgcg cttcttgtgc ctgcggcggc agcggcggga 1080

agactcggct cggctttttt tcccgcaaat tccggcggca gtgcatggtt tacggtattg 1140

accgtaatat tcgggttgat gcccgcactt tcggcagctt ttgcaataga agcgcggctg 1200

atgcctgtgt ttaaaaaacc gcgccccctt ccccgccttt cgctccgcgg aggcatcggg 1260

gaggaaaatc gaacgcttgt tgccgttccg gtgcttataa cgtccgaaaa gtccgtcaga 1320

caggcggcgg aaacattgga agcgcattat cttgcggcgc aggactttgc cgcggcaaat 1380

tgcggcgcag agtttgcaat acttgcggat tttcccgatt ccgccgagaa aacaaagccg 1440

ggagaagcgg aactgatcga gcttgcaaag aaacttatcg atgacctgaa ttcgaggttt 1500

tgcgccggta aaaggccggt gttccattat ctgcaccggg aacgtgtttt caattcggcg 1560

gacggtgttt atatgggcag ggagcgtaag cgcggcgcgg tggaggcact gcttgactgt 1620

gccgcgcacg gggatacgca tgagttttgc tgcaattatc cggatatttg cagcgaaaaa 1680

gaacgcttcc gctttcttgt tgttcttgat gcggacacga taatgccaaa cggagcattg 1740

cgcaggctga ttggtgcggc ggcgcatccg ctcaaccttc ccgtgtttga aaacggtgcg 1800

gcgcgtccgt ccttcggatt cagcataatt gctccgcgca tggcggcaac atcgcgttcg 1860

gcggcggaga gcgggtttgc cgcgctgata agcggggaaa gcggcatgtg tgcatattcc 1920

gttcgtgttt ccgatttcaa ttgggatgtt tttgaggagg ggaacttcgg cggaaagggc 1980

ataatcaata ttgatgcatt cctgcgctcg gtctcgggca aaatcccgga aaataccgtg 2040

ctgagccatg atttggcgga aggctgtttt gcgcgtgcgg catatgcggc cgatattgtg 2100

ctttatgacg gcgaaccgtc cgcattgctg ccgtggcaaa agcggcgtca tcgctggctg 2160

cgcggggata tgcagctcct ccccttcctg tttccgccgc tgaattcggg aattgatgca 2220

gtatcgcggc gaaagatcct gttcaatatc cgcgcggctt tcggaggcat aagcttcgcc 2280

gccgcattcc tgcttgccgc cgttttgggg cttcgcccgc tttttttgct tgctgcaata 2340

acttttttca ttgatttgct gttggaagcc gcattcctgc tgcttcgtct gccctttcga 2400

aagtccgcac ttcgtccgct tgttctgctt gccgggcgtc ggctgtatga gcttgcggtg 2460

ctgccctatt cggctctgcg cgatgcggat gctgtttgcc gtgcgttgta cagaatgatt 2520

ttttcgcacc gaaacatgct gcaatggcag acagcggcgg atgcggagcg cagcagaccg 2580

caaaatacaa gggaatattt caaggcactg tttccgtgcg ccgcattcgg cacaattgtt 2640

ctcgccgccg ccgtttctgc ggcaattgca gcggcacggc agatacacaa cggcgaaacc 2700

gcaggcatgg cagcggcggg tcttgcgacg gcggtgcttt ggctttgtgc gccgctgatt 2760

gcggaaaaga aggatgaaaa gcccgattcc tatcgtttta ccgcagcgga gagaggcagg 2820

ctgacggaaa tttttgccga tacttggcag tactttgaac agaactgcac cgaaaaaaca 2880

tcgtggctcc ctcccgataa ttttcaggaa gagccttatc gcggcgcggc aatgctgact 2940

tcgcccacga atatcggcat ggggttgatg gctgttgttt cggcgcatga tatgcatctg 3000

ctcgatgagc gcggaatgtt cacgcgcctt gaacgcatgg tgaattccat agaaaagctt 3060

gaaaaatggc atgggcatcc ttttaattgg tataacctgc gcgacttatc gctgctgcgt 3120

ccgcgcttca tatcgactgt tgacagcggg aatctgttcg cctgccttat cacaacggca 3180

tgcgcgcttg ctgaatgcgc ggggcaggcg gaaaagacct ccgcagagtt tgcggaagaa 3240

ctgaaaaagc ttgcggcgcg ctgcacagcg atcgcaaggg caatggattt tagggtactg 3300

tatgacaaca cgagagagct ttttcatatc ggctgctcgt tcgaggaggg aaagcttact 3360

ccgtcacatt atgacttgct tgcatcagaa tgcagactga cgagtttttc tgcaattgcg 3420

ttttctcgga ttgggtcgga gcattggttt gcgctttcgc gccttatgtg cgatgcttcg 3480

ggcgggcgcg tacttaaaag ctggagcggg acgatgtttg agtatctgat gccgctgatt 3540

ttctttgaaa ccgttcctta cagtatgcag tttgaggttt gccgcaatgc ggtgctgacg 3600

caaatccttg ctgccgcagc ggaaaaacct tggggtgtgt cggaatccgg gtattatgcg 3660

ttcgatgatg ccctgcgcta tcaatatcgt gctttcggca atcccgaact tgcgcttgca 3720

ccgggcagaa tgcggtcgga tgttattgca ccgtatgcat gcgtgcttgc acttgccgtt 3780

gagccgaaag cagcggcgga gaacctgcgc ctgctctgcc aaatcggcgc ggcgggcaaa 3840

tacgggcttt atgaggcgct cgattacggg gcggctgaaa agaacggatt tgcaattgta 3900

aaaagctata tggcgcacca tcagggaatg agtctgtgcg caatcaacaa cgctttgaac 3960

aacaatgtgc ttgcgcgaag attcatgagc gttcccgagg ttcgagcgaa cgaacagctg 4020

ctgtttgaga acatgcccgt tgaccccatt cgaattaaaa cctatgaaag tgaaattttc 4080

agggagccgc acgcggctcg taatgcggat gaatttgttc gcattatcaa aaaaaacgct 4140

gcggaagcga agcggaacga atcaccgcgg ctgcggggag catttattcg gaagaagata 4200

aaaaacccgg gcaatcggaa aaacgcaatc gcaatgcagc cgaccgaggg gcggacggaa 4260

aataacagcg ggcaggcggt caacggacag attgtcacaa acggctcata cagcatattt 4320

gttgatgaaa acggctgcgg ctattcaaaa tgcggcggtg tgttgataac ccgtctgcgc 4380

ggcaaggcat ggggcgcgtt tggtgaagat gcggcaaacg cttctgaatt cggctttgtt 4440

ccgccgaacg gtgtggattt tgtaattgca aaatatgatt tcgaatcagg cgaaaaagcc 4500

gcaaaacgca tttcgggaag catcacggcg gagccgcaca gagttgtttc ggaggatcgt 4560

ttcgcttcaa ttcgggcacg cctcacttca atggttgctg ctgattcgga ttgcgaaatc 4620

cgcacgctgg agctgattaa ctgcggcaaa aaggaagaaa cggtcgatgt cggcatgttc 4680

gccgaaatcg cgcttgcacc ggcgagggaa tttgaggcgc atccggcatt tgtgcatgtt 4740

tgcaccgaat ccgaaagggc ggacgacaca ttgattttta caatgagaaa aaaaccgggg 4800

aagccgtctt actccgcttt tttcaacgtg gcatcgcttg aaagggtgca attttgcgcg 4860

gatggtttgg tttgccccgg gcggcacaaa agtcatgaag atgctctgct gatgcagtgc 4920

atttcggcag ccgacgcgga agaattgccg acggtgcagc cggttgagcc gtactttttc 4980

gcaaaaacaa aggttcgtct cgggccgggc gaaagcggaa ccgtgcgtct cacgatcggc 5040

ttcacaaaga acaaaacgag aattgatgag attcttcatc ggcaaaggca gcggctgcaa 5100

agtgcggagg ctgcggcgcg aatgcattcg gcgggaatac ttcgtgcatt tgaaatcgac 5160

gcgaaaacaa acgcatttgc acagattttg aatgcgttta tcgtgaaagg cattgtgtca 5220

aagggcaaga gtgatataaa aagccggctt ggcggtattc gcggcctttg gaaacacgga 5280

atctccggcg atcgcccgat cgtgctcatg cgtgtcactc gttcggagca ggtgaacggg 5340

gtgcgtatgc ttttgaatgc tgcgaaatac atcaacatgt gcaatgcagg cagctgcaaa 5400

acggagcggg acgagttcaa cgagggaagt cgcacggacg agcggcagga aaagcttttt 5460

gatgttgttc ttatcggaga atacccgcat gaatatgcga acgctctgcg ggcggcgctt 5520

gaggaattgc gggcaatctg cccaacctgt attcttttgc acggatttga cttgaccgag 5580

tcggatcgcg cgctgattca cgctgcggcg cagattgaaa ttgatgccga tgcaccgctt 5640

gcttttgcag atgactcaaa atatattttt gagcacggca gcataaacga gaaaatggcg 5700

gaaacagaaa cgatgcgccc aatgcttacg cagccgaaca taacggatgc acgaaaaaaa 5760

gcttgcttct catggaacgg ctgcggcggg tttgataatg agaaaatgga atttgttatt 5820

gaaaattcgc ctaaaaacat tactcctttg ccttggagca atgtccttgc cggaaggcgc 5880

atgggtgcgc ttgtgaccga gagcggcggc ggttacacat ggtgcggaaa cgcaagaatg 5940

ctgcggataa caccgtggtc aaacgatgct ttgtttgata caagcggaga atttttgata 6000

ctgtttgata ttgaacgaga aagcaggctc agccttttgc ccgcattcgg cagcagctgt 6060

gttgtgtcgc atgggtttgg gtacagccgc tttgaatggc ggacggatga gctcagagtg 6120

cagctcacgg tgacggttga catggagtta cctttgaaat actcaacggt atgcatcgaa 6180

aacctgacgg caaacagaaa aaaactgcgg ctgcgctgcg gggcggactg ggtgttgggc 6240

gaaaaagcac atcccgcttc actgcaattt gaaactgtcg gcgggaaggg tttcccgctt 6300

tgcgctgcgg cgcgcgacgc actttcggaa cattcggagt ggggcttcct ctccattttg 6360

ggcggttcga aaagccttgc tgcgaacgga aatcaaattg cagccgaatt caccgttgaa 6420

ccgcaatcaa cagcaaagct tacggtgctg cttgggtttg agcagtttga acgcattccg 6480

aagctccttg ccgatgcgga cgctgaagcg gcgttgaata aaacagtgcg gttttataag 6540

caaaaaacag atatgttaag gataaaaaca ggcgatgcga gctttgatgc actcgtgaac 6600

gggcggctgc tctatcaaac gtattcggcg cgcataatcg ggcgaacggg attttggcag 6660

tgcggcggtg cgtttggctt ccgagatcag cttcaggatg cagcagcact tgctgcgacc 6720

gatcccaaat gcacaaggaa tatgataatc aagtgcgctt cacgtcagtt taaaaagggc 6780

gacgtgcttc actggtggca cgaaggtttt gcagacgggc tgccgcgcgg ggtgcggaca 6840

atgataagcg acgatcgcct ctttctgccg cttgcagcgt gcgaatacgc tgagggcagc 6900

ggggatgcag cgatttttga cgaacaaata ccgtatcttg ccgacatgaa gataccgtac 6960

ggacaacaaa acatatattg tgaaatggag atgtccgatg ataaggaaac cctgtacgaa 7020

cactgctgcc gcgccgttga gctttcattt gagctgcgcg gggcgcacgg tctgccgttt 7080

atgggcacgg gtgattggaa cgacggcatg gacgctgtcg gttcggacgg cggcgaaagc 7140

gttgttgtcg gctggctgct gctgcttgcg gcgagggcat tgctgcctgt ttgcaaaaag 7200

cggggagata ctgcacgggc aatgcttttc gaacgtgaat ccgccgcgct tcgtgctgca 7260

attgaaagca atgcgtggga caaaaacaga tatatacgcg cttttttcgg tgacggaaca 7320

agccttggaa gcgcggactg ttcggaatgt tccgttgatt gcgtaagcga atgttttgcg 7380

gttttcgcaa acgccgttca tgcccgcgaa gcgtttgaaa ccgtgcttgc ggccttgacg 7440

gacccggtga acgggctgat tcgtctgctg acccctccgt ttgacggaaa aacgaggaac 7500

gctgtgggct atatagaggg ttacctgccc ggagtacgcg aaaacggcgg acaatacacg 7560

catgcggcgg cgtggtgcat aatagccgca tgtcggctcg gtatggccga tacagcggac 7620

agcttgttta aaatgataaa cccgattgaa cacggatccg caaactcaat cgaacgctat 7680

aaaggggaac cctatgccgt tgctggcgac gtttattcgg tcggaaggct tgcagggcgg 7740

gcaggctgga cgctttacac gggatcggca gcatggctgt atcgcgccgc cgtcgaaaac 7800

attctcggaa tccgcaagag gggaacaaaa ctttttgttg aaccctgcac cgtgcttgat 7860

gcattttcgt ttgaataccg tttcggaaac accgtttaca gtgtcgatat gcaccgcagc 7920

gcttcgaatg caaaggagcg gcacggtgca tgtgttgaac ttgttgacga cgggaaggtt 7980

cataatttat cgcttgaata caggtaa 8007

<210> 10

<211> 993

<212> DNA

<213> Ruminococcus sp.

<220>

<223> isolated from intestinal tract, Ruminococcus sp.

<400> 10

atgattacat taaatgatta tctgtattca ggagatacgg ttttaaaaat tttacagaaa 60

tatacccggg atctgagaaa agaagcgaaa cttactcata atgaaattga tatgatgcat 120

gtgaatttcc tgattcagat cacagagctt ttagaacaca atgatttcct tacggcccag 180

tcccagaaga tccgggaatt ttataaatat atggctcatg aatatccgtt tcttgcgttt 240

acatttaaag gaaggatcaa atcgctgatc cgggcagaag agaaatttaa cggctatatt 300

gtggaatata tttacgatta ttatacaaaa catggaacct atcctcctgt atcggaactg 360

aaaaataaac tcagttgttt tcgggatttt attgcataca gaattgtgtt gtgtatgccc 420

aagtgtcatc tgaaaccagg agaagatcag gaaacggcgt cgatccgcta tctttatgag 480

attgccaatg ttcttcccgg gttcctggaa gaacgagggt ttacggtgga gtctgcctat 540

ggagtaaaga aaagtacgtc gccgcttctt aatgaagatg tgaagatgta ttaccgggat 600

tatatctgtg gaaccagcgg ggaaggatat cagtcattac atatcacagt ttatgataac 660

agttcccgtt ctttcatgga ggtgcagcta cgtacccaga aaatggatga cactgcggaa 720

attggtccgg cgaatcatct gggatatgag aagaaacagc aggatgagcg tgcaagacgt 780

gatgctattc cagaaggcga gtgcgtttat ttcgatgaag cctatgaacg gggcatgcgt 840

cttttgcagc tggaactggc agatctggat gtaaatatgt tttccgcagt agataacagt 900

ctgatcaatg atgggtgcgg actgttcagg ggaagactga tattacctta tgaacatttg 960

tcaagattac agaatgatgc cattattgaa taa 993

<210> 11

<211> 990

<212> DNA

<213> Megamonas funiformis

<220>

<223> Megamonas funiformis isolated from intestinal tract

<400> 11

atgtttgatt tttttgttga acaaatacta ggaatgcaat ggctgaatga tttagtttgg 60

caagtcttag taaattattt taatgcagat aaaagttcaa attggtggtc tagtattcat 120

tttttctttt atgatacgat aaagataact atcttattag taactttaat ttatttgata 180

tcatatattc agtcgtattt tccaccagaa aagacaaaac gaattttaac gcattttgat 240

ggttttactg gaaatataat tgctgcacta cttggaacag taacgccatt ttgttcttgt 300

tcaagtgtgc ctatttttat aggttttact agagcgggat tacctgtagg catgactttt 360

tcttttttga tatcttctcc tatggtagat gtggcatcta tgcttatgtt gatgtcattt 420

tttggacctg aatttgctat tttttataca gttgtaggcg taatattggc tgttgttggc 480

ggtaaagtct tatcgataat gaaggttgaa aaatatttga aaaaatatga tggagcaatt 540

agagattttt atgctgaatc agaatatttt aatcaaagac aaagattaaa ttatgcttta 600

aatgatacta aaattatcgt gagaaaagtt tttccttatg tattattggg cgtattaata 660

ggtgcagtaa ttcataattg gataccacaa gaatttattt taagtatttt gggagataat 720

aatccttttg ctgttatttt ggctacaatt attggtatac ctatttatgc tgatatattt 780

ggaactttgc caatcgctga agctttatat ttagtaggtg tgccagctgg tacgatttta 840

gcattgatga tggcgataac agctttatca cttccatcac ttattatgct tagtcaagta 900

ttacagagaa aattgttatt tttatttata ggcattgtaa gtataggaat tatttttgta 960

ggctatattt ttaattttgt ttttggttaa 990

<210> 12

<211> 798

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 12

ctggccgctg ttgtaggcaa ggaaaagctg gcctgcatcg actgcgtcac cgacgctctg 60

accaagcgca tcgagttctt tgccgagatg ggctgccgcg cttcggacca cggtctggat 120

tacatcccct accgtgaggc caccaaggaa gaggtcaata ccatctacca gaaggtcgtg 180

gccggcgaga gctgcaccgt ggaagaggcc gaaaagtacc agacctacat cctgatccat 240

ctgggcaagg agtatcaccg cctgaacatc gccatgcagc tgcacttcaa ctgcctgcgc 300

aatgtcaacc gcaagcagta tgcaaagttc ggcccggata ccggctttga catgatcaac 360

accgcttcct gcggcggcga gatcgcagcc ctgctgagcg ctctggacga gaccaacgag 420

tgccccaaga ccatcatcta cagcctgaac ccggccgaca acgagcagat cggcaccatt 480

ctgggctgct tccagagcac cgaggtgccg ggcaagatcc agcacggttc tgcatggtgg 540

ttcaatgacc agaagatcgg catggagaac cagatgaaga gtctggcaaa tctgggcctg 600

ctgggcaact tcgttggcat gctgaccgac agccgcagct tcctgagcta cacccgccac 660

gactacttcc gccgcatcct gtgcaacctg atcggccagt gggtcgagga tggcgagtat 720

cccaacgacg agaaggctct ggagaagatc gtcaagggca tctgcttcga caacgcaaag 780

cgctacttca acctgtaa 798

<210> 13

<211> 2604

<212> DNA

<213> Bacteroides sp.

<220>

<223> isolated from the intestinal tract, Bacteroides sp.

<400> 13

atgaaaaaca ggagcattac ctacctatta ctatttggta tttgcagcat gttggcaatc 60

atgcaatcgt gccagaaaac agatgacctg gaagcagaca tcaattctct gaaagaccgt 120

gtcgctgctt tggaaaaagc aacggaagga ctgaatactt catttgccag tctgcaagcc 180

cttatgcaaa aaaacaaagt cattatcgga attactccga ctaaagatgg tctgggatat 240

ttgctggaac ttagtgacgg aacaagcatt aaagtcatgg aaagtgaagc agtacaggcg 300

tctgtccctg aattttccgt agacgaagaa ggctactgga tatataaaac aagtaatgat 360

accgatttca aatatctgcc aggagccgat ggagagaaag tatcagcatg gccccgtgat 420

gaagaaggca atgtggtggc aactccatta atcagcgtat cttccagtgg atattggcaa 480

gtgagctacg ataatggaca aacgtataca agcttaggaa ctaaggccga aggtggaagt 540

cagggaggaa ccagtatatt cagtaaggta gaatataatg aagcaaacca tactttctca 600

ttcacattat ccgatggagg aaaaacatat acctttccgg tagatgacac ctttggactg 660

ataatttatg gtctcaatga tgccaacagc gaacaagctg tgcaggtttt cgtccccaat 720

gagaatcata aggaatataa agtagaacag aatgacgtac aacaagccgt agtacaagct 780

cccaaaggat ggaatgttct tttatccgag aacttgttaa ctatcacacc acaagcaact 840

gcgacaaagg atgtggaaga aactattaaa attgtactga cttcatcaaa gaattatatc 900

cgtattgtat ctattgaagt taaacaactt tccagcgaag ctggagcaga agcctggcag 960

cagtttgtta acgctgacca acagaatgtg ttattagact tttcatacgc cggctataag 1020

tatggtgaaa tggctcctcc cgaaatagaa acgctaacag cgcaaggata taaagtatac 1080

gatgttacag atcccaaata tggagctatc cccaacgacg gcaaatctga cagagtagct 1140

ttcatgaagg ttttggaaga aatagcgagt caaacaaagc aagaagacaa caatatgacc 1200

gaccgatata tcaaagaaaa tgctaaagct attatctact tccccgaagg aaattatatc 1260

ctgcaagatg agggttctaa agatcgcagg attcgtatat cgatgagtga tattgtttta 1320

aaaggagccg gaaaaaacaa aacgacactc gaaatgaccg ctgctaataa ttctcccaaa 1380

ccaaccgaag agatgtggaa tgctcctgtc atgatggagt tcaaacacaa tacaggatta 1440

aaagaatcaa taggtgttat cactgaagat gctcccatag gcagcagaac cataacggcc 1500

agtttaaccg gagtaagcgc aggcagttgg gtatgccttg tactgggaac cccagagttg 1560

ggaaacacga atgatgatgt tattaattcc gaactatctc cctatcgatg gcaagatatt 1620

aaagtacagc aaggcactac tcccaatatc aaaacaaatg gtattcaaat attcgaatac 1680

catcagattg aaaaaatcag cggcaatagt gtcaccttca aagaaccgat catgcacgcc 1740

atcaataaag attggggatg gaatgtacat aaattcgcca attatgccaa tgttggtgta 1800

gaagatctta cattcaaagg acatgccaaa gagaagttca ttcaccacgg ctctgacatt 1860

gatgatggtg gttttaagct catcgatttt gtgcgtctga ccaattcgtg gatgcgccgg 1920

gttaattttg agagtgttag tgaagccatg tcaatcacca gttctgccaa ctgttctgct 1980

tatgacatca ctattggcgg taatcgtgga catgcctcta tccgttcaca ggcttcttcc 2040

cgcattttta ttggaaaagt aactgaaaac agcaatggct acacacttag aaaaggagaa 2100

ggtgaaagca cattgatgga atataaaacg aacgttggcc aatatcacgc ttgcggtgtt 2160

tcaaagcaaa gtatgggtgc tgtaatatgg aatgttagat ggggagatga ttcttgcttt 2220

gaatcacatg ccacacagcc acgtgcaaca ctaatcgact gctgttccgg tggtttcatg 2280

cattggagac aaggaggaga ctctgctcaa atgcctaatc atatggagaa tctgacaata 2340

tggaatttct acgcaacaaa tgcacaaaca gaccaggaca tcgatacaga aggtaaattt 2400

acctggtggg atggtaacgg cttttggtgg aaatttatgc ctcctatcat tgtcggtttc 2460

cacggaagtc ctttagattt tgatgatact caaatgaagc gactcgaaag caacggaact 2520

gctgtggaac catattcact ctatgaggct caattacgta aacgtttggg atatgttcct 2580

tcctggctat cctctttaaa ataa 2604

<210> 14

<211> 333

<212> DNA

<213> Bacteroides ovatus

<220>

<223> isolated from the intestinal tract, Bacteroides ovatus

<400> 14

gaggaaagtc cgggcaacat agagcatcct acttcctaac agaaagctgt ccgcgagggt 60

agagtaacgt agaagaaaat aaccgccgct cctttcgggg agaggtaagg gtgagaaggt 120

ggggtaagag cccaccagtc gcatggcgac atgcgggctg tacgtcttag gagttgtaag 180

gtcatgtaaa ccggcgttat gagagttgct cgctccatgt cggagggtag accgctaaag 240

tgtcgtggtg acacgccact cagataaatg acaggcacct gatataatta aagattaata 300

atcggaaatt atatgggtac agaactcggc tta 333

<210> 15

<211> 1152

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 15

gtgacccttg cccccctgaa aggaggaaac gccatgtatc gaagcgtatt ggcccttctt 60

ttcgcctcgg tactgctgct cagcggctgc gccgcagaac ggcagcgtgt accgcgggaa 120

aaggacacgc aggaagcgga gaaaaccgga cagtcctcgg atatgtccgg cgaggagggt 180

atatatatgg acaccacgga aaatgtcatc tatctggcag gcggctgctt ctggggcatg 240

gagcagctca tgcaatccat ccccggcgtc atcgacgccc aaagcggcta tgccaacggc 300

acctgcgagg cagacgccac ctaccagacc gtctgcaagg gcgacaccgg cttccgggag 360

accgtgcggg tggaatacga ccccgagcag gtgagtctcg acgcgctgct gctggcttac 420

ttctatgtga tcgaccccac ggtgcagaac cggcagggca acgaccgggg cagccagtat 480

cagaccggcg tgtactacac caacgacaag gtcagggaaa cggtggagcg catcgcaggc 540

attgagcggg gccgcagcga gaaattcttt gtggagatcg gtccgctgaa gaattactat 600

cccgccgagg aatatcacca aaactaccta gaaaagaacc ccaacggcta ctgccacatc 660

ccccgggcgg agatggagct gttctccagg cttcgcatcg atccgggcga ctaccggaag 720

cccgcggcgg agaccatccg ggacacgctg acggcggagc agtaccgcat cacgcaggag 780

agcggcacgg agcgcgcctt taccggtgag ctctgggaca agtttgaaaa gggcatctat 840

gtggacatcg tcaccggcga gccgctgttc tcctccacgg ataagtttga aagcggctgc 900

ggctggcctg ccttcacaaa gcccatcgag agtcctgctg tggtggaacg ggaggacttg 960

agccacggca tgcgccgcac agaggtgcgc agccgcgccg gtgactccca tttgggtcat 1020

gtgttcaccg gcgacccgga gtcgcccaac ggtgtgcgct actgcatcaa cagcgccgcc 1080

ctccgcttcg tgccttacgc gaaaatggag gccgagggct acggatacct cctgaatttg 1140

tttgagggat ag 1152

<210> 16

<211> 276

<212> DNA

<213> Bacteroides coprocola

<220>

<223> isolation from intestinal tract, bacteriodes coprocola

<400> 16

gatatgaaaa caactaagat tcaattcaac ggcaggtctt attactcccg catcgtggaa 60

tctgtcgatg gcgaagaatt gcttatcggt tcgacaatcc tgctcgatgc cttgcagccc 120

ggcagtttca atgacgagaa cgaaggtttt gccagtaaag aagccgaaag gatttatgac 180

gagattttct tctttaccga cgagagaacg cttcagcttc ccgaaaacga gttgatagcc 240

gaactcaaaa aggataaccc cgattggttc gagtaa 276

<210> 17

<211> 1023

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 17

atgagtaatg aagttgaatt aaagcttgca gtcacccctg acgccttcga tatcctaaaa 60

acacacctcg atcaattcaa tatcttagag caaaatacga tctttctagg aaatacttat 120

ttcgattatc ccgatcattt tttggcgaaa caaaaaatgg gattgcgtgt ccgtcgggaa 180

aacaacgatt tcacacttac cttaaaaact gacggaaaag tagttggcgg attgcatagt 240

cgtccagaat ataatttatc tatacctgat gattcagtac ctacatcggc acaattaaca 300

tcattgtatc catttgaaca tttgccttcc tctacattac agccgatttt ctcaacagat 360

tttaaccgca ctttttggtt gattgcattt ggtgcatcaa aaattgaagt ggcttttgat 420

caagggaaga ttctgttagg agagaaaaca cagcctatct gcgaaattga atttgaatta 480

aaagaagggc ttgtttcaga tcttttctat tttgtctcac ttttaccttt tgaacaggat 540

gtgtatttta gttcggcaag taaagcgaaa cgaggctatc aactaggtag taaacagctt 600

ctgattgatt ggttaaataa atggcgtgat ttcttaaaag aagaacgtga gggaagtgca 660

gttgattctc gcgaacaatt aagtgcagtc atgaaaatgg aacagcaatt gattgaggaa 720

acgctttcat tcccaacgga tgtgtttgct ttcgatttta tgaaaaccgt tgaacgtgtc 780

ggcgcatttt tcaatcttta tcattattat gatgacaaca aagccttgtt tgaaaaattg 840

gaagaaaatc gatcagctga gttattagta agtaatcaat ttttccttaa tgaaataaaa 900

ggcttgatta cctttcatag tcaaacaaaa gataattttc agaccgttga gaagttaaaa 960

gcgttattaa agactcgtgc gtattttgaa agaatgcttg gcttaatgat gttgatggcg 1020

taa 1023

<210> 18

<211> 1989

<212> DNA

<213> Bacteroides xylanisolvens

<220>

<223> isolated from the intestinal tract, bacteriodes xylanisolvens

<400> 18

atgaaacaag aagaagacaa attcaccggg ttacccgaga atgcgttcag agagttgaaa 60

cctggggaag tgtacaaccc gttgatgggt ccctctaaaa attatccgga agttaatatc 120

tggtcagtag catggggtat tgccatggca atcctttttt cagccgccgc tgcctatctg 180

gggttgaagg tcggacaggt atttgaagca gctattccaa ttgcgatcat tgccgtagga 240

gtttccggag ctgccaaaag aaaaaatgcg ttgggcgaaa atgtgattat ccagtctatc 300

ggagcttgtt ccggagtaat cgttgccgga gcgatcttca ccctccccgc tctttatatt 360

ctccaagcta aatatcccga gatgacggtt accttcatgc aggtattcat cagctcttta 420

ttgggtggcg tattgggtat tctattcctg atccctttcc gtaaatactt cgtcagcgac 480

atgcatggaa aatatccttt cccggaagca acagctacta cacaggtgtt gatctccggc 540

gaaaaaggcg gcagtcaggc caaaccgtta ttgatggcag gtatgatcgg cggattatat 600

gacttcatcg tagcgacatt cggatggtgg aacgagaatt ttactacccg tgtttgcagt 660

gccggagaaa tgctggcaga aaaggcaaaa ttagtattca aggtaaacac cggtgccgct 720

gtgcttggtt tgggatatat cgtcggactg aaatatgctt ccatcatttg tgccggttca 780

ctggctgtgt ggtggattat cattccggga atgtctgcta tctggggtga cagtgtactg 840

aatgcatgga atccggaaat cacctctacc gtaggtatga tgagtccgga agaaatattc 900

aaatattatg ccaagagcat cggtatcggc ggtatcgcta tggcaggtgt gatcggtatt 960

atccgttcct ggagcatcat caagagcgct gtcggactgg cagccaaaga gatgggcggt 1020

aaagggaatg tagagaaaag cattatccgt acccaacggg atctttccat gaagattatc 1080

gctatcggtt ccatcatcac actgatatta atcgtattat tcttctatct cgatgtcatg 1140

caagggaatc tgttgcatac attggtagcc attgttttgg ttgccggcat ctctttcctg 1200

ttcaccacag tagcagctaa cgccattgcg attgttggta ctaatccggt ttcgggaatg 1260

acattgatga cgctgatttt ggcttccgta gtgatggtag ctgtcggact gaaaggtcct 1320

tcaggcatgg ttgccgcatt ggtaatgggt ggtgtagtat gtaccgcact gtcgatggca 1380

ggaggtttta tcaccgactt gaagattggt tactggctcg gaagcacgcc tgccaaacag 1440

gaaacatgga agttcttggg aacaatcgta tcggctgcaa cagtaggcgg tgtaatgatt 1500

atcctgaata agacatacgg atttacaagt ggcgcactgg ctgctccgca agcaaatgcg 1560

atggcagccg tgatcgaacc actgatgagt ggtgtaggtg caccctggtt gttatatggc 1620

attggggctg tcttagcaat tattctgact ctttgtaaaa ttcctgcatt agctttcgca 1680

ttgggtatgt ttattccatt ggaactgaat gtgccgctgg tagtgggtgg agcagtgaat 1740

tggtttgtta caactcgtag caaagatgca tctctgaata cggaaagagg tgaaaaagga 1800

accttactgg catccggttt cattgccgga ggtgcattga tgggagtaat cagtgccgca 1860

atgcgctttg gtggaatcaa cctggttaat gaaacatggt tgaacaatac ttggtcagaa 1920

gcgttggcat taggagcgta tgccttattg attctttact ttataaaggc ttcaatgaaa 1980

gttaaataa 1989

<210> 19

<211> 525

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 19

atgaagctca atctcgatcg attcaccgcc gcgcagctgg ctgcgcacgt tgccgccggc 60

gatttctctg ctaccgaggt cgcgcgggcc tcgctcgagg ccatcgaggc acgcgagggc 120

ggggtccagg cattcctgca ggtggcgccc gagcttgcgc tcgaggccgc cgcgcgcgtg 180

gatgccgacc gtgccgcagg caagccgctg cccccgctcg cgggcgtgcc gctggccatt 240

aaggacaaca tgaacctcgt gggcacgcgc accacctgcg cttcccgtat gctcgaggac 300

taccagagcg tctacaccgc cacctgcgtc cagcgcatgc tcgacgccgg ctgcctgccc 360

atgggcaagg ccaacatgga cgagtttgcc tttggcagct ccaccgagag ctcggcgttc 420

catcgcacta acaacccctg ggataccgag cgcgtgcccg gcggctcctc gggcggctcc 480

gctgcagccg tcgccgcggg cgaggtcgcg ctctcgctgg gctcg 525

<210> 20

<211> 396

<212> DNA

<213> Bacteroides intestinalis

<220>

<223> isolated from intestinal tract, Bacteroides intestinalis

<400> 20

tcctccggtg ataagtggca ttttcatcac caaccaggat acgaatatca gtatcgccca 60

aaagaaaaga aaaagaggaa tatgccagta ccactccggg cgggaagcaa aaaattcact 120

tctcaggtta aagaaataat agaggcaata atgataggga acagccacca gccccgtcag 180

caatcctacg aagatgctga cgaaatacag gcgtgcgtca accagtttca gcttcacgac 240

ataccacttc ctcagttttc taaagttttt gaacctttga aacattcctc agatttttct 300

tttattaatt tgagtatcta caacaaatta accctgttga agtttcaaga ctttgcgttt 360

tttacttttt ccccatcata ttcaatcgca ggatag 396

<210> 21

<211> 849

<212> DNA

<213> Faecalibacterium prausnitzii

<220>

<223> Faecalibacterium praussnitzii isolated from the gut

<400> 21

acggcggaga tcggcgacaa ctgcaccatc taccacggcg tgaccctcgg cggcaccggc 60

aaggacaccg gcaagcgcca ccccaccctg ggcaacaatg tccttatcgg cgctggcacc 120

aaggtgctgg gtccggtgta tatcggcgac aatgcccgca tcggcgcggg cagcgtggtg 180

ctcaagaacc tgcccgccaa ctgcaccgcc gtcggcgtcc ctgccgaagt ggtgcgcatc 240

aacaacaagg ccatcaaccc cgccgacgat ctcgaccagc aggatctgcc ggacatcatg 300

gcccagcgtc tcatcgacct cgaccgccgc atcggtcagc tggaaaggcc gcacagggcg 360

atattccccc cacggcccag caggtggccg cacggcagcg caagcattaa aaaacggaag 420

ctttccctcg ggagagcttc cgtttttctt ttatcatatc aactctttga ccttctccac 480

cgctgctgct accagctggc cgatgggctg gtccggcacc cccaccacga tgttatcaca 540

gtggttgatg gggatgagca cccgcgctgc actgctctgg cagaccgccg ccgccatggc 600

cggggtgatc tcacccagca gcgaatcggc gatgacgatg ccgatggggc cgacgatgat 660

gtccgcccgg cggcagttga ccaccacggc attttcgccg gtggcggcgc ggtgtgcgcc 720

cgccttgagc atagccgaag ccgcgatgct gttggttcct acggcggtca gctcgatgtc 780

cgggcatccg gcggcaagcg ccgccgtcag ctgacggcca agaccgccgc cctgcccgtc 840

aatgactaa 849

<210> 22

<211> 672

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 22

ttttgtggga aatatgtgaa agtgggagtc tttttgactg agataaattt ccttgcagtg 60

cgtctttatt catggcatac tataaggaag tgcagtgtac cgtggtttct gaaacaaaga 120

ggtcagattc cggggggtac atgggtggaa tatggaggaa tgagaagcga tatgaagaaa 180

aaaggaatgg tgctttttct gaccggtctg ctgggcatgt cccttttcct gggcggctgt 240

accagcaaat cagagaagaa ggcactggaa tataagcagc ttggtatcag tcagatgaaa 300

gaagctaaat atgaggatgc ggtcagcagt tttcagaagg ctctggatca gtctctggga 360

aggatccgtg cacaggaatt ggatgtgtgc tactacaagg cactggcaca gtacaaatcc 420

ggagatacca aggcagccat caagacttat gacgaactgg tggattatga taagaaaaac 480

tgggaagtat actatctcag aggaagtgta ttgctggcag acggacagcc ggatgcctgc 540

ctgaaggatt atgataaggc agtgtccctg aatgattcgg atgcggaact gtatggacat 600

atcagcgaga atctgcagaa tgcaggcagg acgcaggaag cacagaaata tctggagcag 660

gggttgaaac tg 672

<210> 23

<211> 1554

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 23

atgacactgg ccttatttca aaaccagacg cttcctgccc gcaggccttc cgaggatgcg 60

cggcagtatg cctaccgtat tatacgaaaa ttcatccttg agcttcatct tcttcccggc 120

cagaagatga atgaagcgga tcttgcctca tctctgaata tgagccggac tccaatccac 180

gattcgtttt tcaaattatc cagagaaaat ctggtagacg tcataccaaa gcggggcgca 240

tttgtctcca aaatcgacca gaaacgcatt gatgactctg tatggattca tcaaaagctg 300

ggaagcgcca tgatccattc catttatata agaaaggttc ccaaatccga gctggagctt 360

ctgtactgtc ccatccagca gatgaacagc tatatgctta acggaaattc agctcagacc 420

gttcagcttc tgtcggaata ctaccatcag ctttatatgc tggccggaaa tctggatctg 480

gtatggtctt ctcttcagaa aaccgatacg gatttgagac gcctgctgtc tctggctgta 540

agcagcccca atgtactgga aggcttgatg caggagctga cgaatctgac agacgccctt 600

ataaaccgtg attatgacaa tgcggggctg atctatgacc accatttctc cagaatccgg 660

atgcttacag cccctctcag aacccatacc ccccagtact ttacagaacc tgtgctgcca 720

gcgcttctcc acagagttct gatttatcct cttaaagaaa ggcggtatca cattatgact 780

gactatcatg cattattttc tctcaaagga aaaacagcac tgatcaccgg aggaaccggc 840

ggtttgggaa gtgcgattgc cagagcattt cttcagcagg gcgctcaggt cgctgtctgc 900

ggtacccgtc cggaaaaagc agctcccata gaagaactgg ctaaaagcct ggaccgtcca 960

tttttatccc tctcctgcga tctgactgat acagaaagca caaaccagat gctcaactcc 1020

atagaacaca ccttcggcac tgtagatatc cttgtaaaca gtgccggaat caaccgtctg 1080

atccctgctg aggaatatga cgaccagacc ttccagacgg ttatggatat aaacatcgcc 1140

tctctccacc ggattacccg tgaagtagga aaacggttta tgatccctca aaaacaggga 1200

cggattctga atctttcctc tgtaaaaagc ctgatcggaa caaagcagga ctacattgcc 1260

tactgtacaa gtaaaggcgc tgtcaatatg tatacccgcc agctggcctg tgaatgggga 1320

aaatacggca tcacctgcaa cgccattgct cccacttttg tatacactcc cattaatgca 1380

ttccagctgg atgatccggt attttacaaa acattaaccg aacgtattcc ccttggaaga 1440

atcggcaaag agcaggatat tgcttctgct gccctttatc tgtgcagcga cgccgcctct 1500

tttgtaaccg gacagattct ctgcgtagac ggaggtctga ctgctatgca gtaa 1554

<210> 24

<211> 786

<212> DNA

<213> Dorea formicigenerans

<220>

<223> Dorea formicenerans isolated from the intestinal tract

<400> 24

atggcggata agaaaaaaga acaggcaatg gttgtttctc aggagcagat cgctgatggg 60

attttcagta tgtggattca tacagaggca gcgcagagcg ccagacctgg acagtttatt 120

tctatgtata caaaggatgc aacaaagtta ttgcctcgtc cgatcagtat ctgtgagatt 180

aacaaagaag acagtcgcct tcgtgttgta tacagagtga ctggagagaa tacaggaaca 240

gaagaatttt caaagttaaa aacaggagat gcaatcccaa tcatcggacc tcttggaaat 300

ggatttccgt ttgaagcggc tgaaggaaag aaagtatttc ttatgggtgg aggaattggt 360

gtaccaccga ttctggaact tgcaaaacag atgaaatgcg acaaaaaaca gattgtcgta 420

ggctatcgtg atgcacagac atttttaaag gaagagttcg agcagaacgg tgaattatac 480

atttccacag agaatggaag tgtcggaaca acgggaaatg ttatggacgc aatccgtgaa 540

aatggactgg aagcggatat gatctatgcc tgtggaccga ctcctatgct tcgtgcgatc 600

aagcagtatg ccgaggaaca taagattgta tgctatatat ctctggaaga gcgtatggca 660

tgtggaattg gtgcctgtct tggctgtgtg tgcaagacaa aagagaaaga tgcccacagc 720

aatgtgtgca acaagcgaat ctgtaaagat ggtccggtat tccttgctac ggaggtggaa 780

atctaa 786

<210> 25

<211> 1485

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 25

aataaaacca tgtcagactt ttcaagaaga aaattcctta aaacgggagc cgccgcttta 60

gcaggcatca ccattgctcc cagctctatc ttaggtatga gccacgggca cgtttcacca 120

accgacaaac tgaacctcgc agccgtaggt atcggaggta tgggacacgc caacatcaac 180

aatgtgaagg gcaccgaaaa catcgtagcc ctctgtgatg tggactggaa atatgccaaa 240

ggcgtattcg acgagttccc caaagctaag aaatactggg actaccgcaa gatgtacgac 300

gaaatgggca aatccattga cggtgtcatc attgccactg ccgaccatac tcacgccatt 360

atcacggcag acgccatgac aatgggcaaa catgtatatt gccagaaacc gctgacacac 420

tccgtatatg aatcccgtct gctgaccaga cttgccgctt ccaccggagt agtaacccag 480

atgggtaacc agggagcatc aggcgaaggt accgaccttg tttgcgaatg gatctggaat 540

ggagaaatcg gtgaagtgac taaagtggaa tgcgccaccg accgtcctat ctggccgcaa 600

ggactgaatg cacccgaaaa ggcggaccgc atccccaaca cattgaactg ggatcttttc 660

accggacctg ccaaactgaa cccgtacaac aacgtttatc atccctggaa ctggcgcgga 720

tggtgggatt acggaaccgg cgcattgggt gacatggcct gccatatcct gcatcagccg 780

ttcagagctc tgaaactggg ttatcctacc aaagtggaag gttcttctac attattactg 840

agtgcctgcg ctcctcaggc ccagcatgtg aaaatgattt tccccgcccg cgacaatatg 900

ccgaaagttg ccttgccgga agtggaggta cactggtatg acggcggtat gatgcccgaa 960

cgtccgaaag gtttcccgga aggaaaacag ctgatgggcc ccggtggcgg tctgaccatt 1020

ttccacggaa caaaggacac attgatttgc ggctgctacg gagaacaacc tttcttgttg 1080

tcaggccgtg tgcccaacgc accgaaagta tgccgccgtg tcaaatgttc ccatgaaatg 1140

gactgggtac gtgcttgcaa ggaagacaaa tccaaccgcg tgatgccgaa agcggatttc 1200

tccgaatcgg gaccgatgaa cgaaatggta gtaatgggtg tattggctat ccgtctgcaa 1260

ggcttgaaca agacactgga atgggacggc gccaacatgt gcttcactaa tatcggtgac 1320

aatgaaacac tcagaacctg tatcaaagac ggatttacga ttcatgacgg tcacccgtcc 1380

ttcaacaaga cctggacaga tccgatcaac gcgaaacaat tcgcagccga actggtgaaa 1440

cataattatc gtgaaggctg gagactgccc gatatgccac gataa 1485

<210> 26

<211> 1827

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 26

atgacaaaac ccgaaagcaa gaccgctctg aagtggatgg acgcggcaag cgccctcacg 60

aaaaacgagg ccgttctcca gtggatggag gaaatggccg ccatgacgca gccggacaag 120

atcgtctgga tcaccggtga ggaggaacag ctcgaggcgc tgcggaagca ggccgtcgag 180

gagggcatcc tcatcaagct caacgagtat aagctccccg gctgctatct ccaccgcacc 240

gacccggacg atgtcgcccg cgtcgaggac cgcaccttca tctgctgtga gcgcgaggag 300

gacgccggtc ccaccaacca ctggatggac cccaaggaaa tgtacgagaa gatgtacgct 360

ctcgccgacg gcgcctacaa gggccgcacg atgtacgtca ttccgtactc catgtccatc 420

atcggctcgc cgttcgccaa gtacggcttt gagctgacgg actccatcta cgtcgttttg 480

aatatggcga tcatgacccg tatcggcaag gccgtgtgcg acgcgctcgg cgacgatacc 540

ggttttatca agggactgca ctgccagtgc aatctcgata aagagaacaa gtacattgtc 600

cacttcccgc aggacaatac catcatttcc atgaactcca actacggcgg caacgtgctc 660

cagggcaaga agtgcttcgc cctgcgcatt gcctccaacc tcggccggaa ggagggctgg 720

atggcggagc acatgctcat cctcggcatc cagaatcccc gtggcgagat caagtacatc 780

tccgccgcct tcccctccgc ctgcggcaag acgaacctcg ccatgctcat cccgcccgag 840

ggcctgcagc gctggggctg gcgcgtgtgg tgcgtgggcg acgacattgc gtggctgcgc 900

atcggcaagg acggccgcct ctgggcggtg aaccccgaaa acggcttctt cggcgtcgcg 960

ccgggcacct ccatgaagtc caaccccaac gccctcatct ccacgcataa ggacaccatc 1020

ttcaccaacg tcgccctcga cctgagcgac aacaccgtgt ggtgggaggg gctgaacaag 1080

accccgcctg cccgcgcgat cgactggcgc ggccgtccct ggacgccgca gagccccgaa 1140

aaggcggcgc atcccaacag ccgcttcacc gcgcccgccc gcaactgccc ctgcctcagc 1200

cccgagtttg acaatcccga gggtgtgccc atcagcgcca tcatcttcgg cggccgccgc 1260

gccaagaccg cgccgctggt ctaccagtcc ttcaactggc agcacggtgt gttcgtcggc 1320

tccatcatgg cgtcggagac caccgccgcc gcgaccggtc aagtcggcgt cgtgcgccgc 1380

gacccgatgg cgatgctgcc cttctgcggc taccacatgg gcgactactg gcagcactgg 1440

ctggatatgg gcaagaagat cccgcacccg cccaagatct tcaacgtcaa ctggttccgt 1500

accgacgacg agggtcactt cgcctggccc ggcttcggcg acaatatgcg cgtgctgcag 1560

tggatcatct cccgcgcgga cgatgagatc ggcgcggcgg agaccgcgct cggctacgag 1620

cccaacaccc acgacatcga catggagggt cttgaaatgt ccatgtacga tatgcgccgc 1680

ctgctctccg tcgaccgtga cctttggctg caggagtgct ccgacgcccg cgcctactac 1740

gagaagatcg gcaaggtgcc cgaggagctc tacgaggagc ttgacgccct tgagatgcgt 1800

ctcaaccgcg gctacaagag aaagtaa 1827

<210> 27

<211> 4293

<212> DNA

<213> Bacteroides intestinalis

<220>

<223> isolated from intestinal tract, Bacteroides intestinalis

<400> 27

atggctttta gaaaagataa caagataaag agtaatttct cgaagatctc tattggtttg 60

gcttctccgg aagaaatcct tgagaattcg agtggtgaag ttttaaagcc tgaaactatc 120

aactatcgta cgtataagcc tgaacgtgac ggtttgttct gtgagcgcat ttttggtcct 180

atcaaggatt atgaatgtca ttgcggtaag tacaaacgta tccgttacaa aggtattgtc 240

tgcgaccgtt gtggtgtgga agtgacagaa aaaaaagtac gtcgtgagcg tatgggacac 300

attcagttgg ttgtgccggt agctcatatc tggtatttcc gctcacttcc taacaagatt 360

ggttatttgt tgggtcttcc tacaaagaaa cttgattcta ttatttatta tgagcgttat 420

gtggtaatcc aggctggtgt gatgtcaggt gagattgctg aatatgattt gctctcggaa 480

gaagaatatc tggatttgat ggagaagttg ccgaaagaca atcaattcct ggaagattca 540

gatcctaata aattcatagc taagatgggt gctgaagcca tttatgactt gctggcccgt 600

ctggatttgg atgcattgtc ttatgaattg cgtcaccgtg ctggtaacga cgcttctcaa 660

caacgtaaga atgaggcttt gaagcgtctt caggttgttg aatcattccg tgcatctcgt 720

ggacgtaata agccggaatg gatgatcgta cgtattgtac cggttatccc acccgaactt 780

cgtccattgg ttccgttaga tggtggtcgt ttcgctactt ctgacttgaa tgacctttat 840

cgtcgtgtaa ttattcgtaa taatcgtctg aagagactta ttgagatcaa agctcctgaa 900

gtaattttgc gtaatgaaaa acgtatgttg caggaagctg ttgattcttt gttcgataac 960

tcacgtaaat ccagtgcagt aaaaacggat gctaaccgtc ctttgaaatc tctttctgat 1020

agtttgaaag gtaagcaagg tcgtttccgt cagaacctgt tgggtaaacg tgttgactat 1080

tctgcacgtt cggtaatcgt tgtaggtccg gaattaagaa tgggtgaatg cggtattcct 1140

aaattaatgg cagctgaact gtacaaaccg ttcattatac gtaagttgat tgaacgtggt 1200

attgtgaaga ccgtgaagag tgccaagaag attgtagatc gtaaagaagc tgttatctgg 1260

gatattctgg aacatgtaat gaaaggtcat ccggtactgt tgaaccgtgc accgacattg 1320

caccgtttgg gtattcaggc gttccagcct aagatgattg aaggaaaagc tattcagctg 1380

cacccgttgg catgtacggc tttcaatgcc gactttgacg gtgaccagat ggctgttcac 1440

ttgccgttga gcaatgaagc tgttctggag gctcagatgt tgatgcttca atctcataat 1500

attttgaacc cggcgaatgg tgctcctatt actgtgcctt ctcaggatat ggtgcttggt 1560

ttgtactaca ttactaaatt acgtaagggt gcaaaaggcg aaggcttgat attctatggt 1620

ccggaagaag ccttgattgc ttacaatgag ggtaaagttg atattcatgc tccgatcagc 1680

gttgttgtga aggatgtgga cgagaatgga aacgttgttg acgtaatgat gcatgacacg 1740

tctgtaggtc gtgtaattgt taacgaaatt gttccccctg aagctggtta tatcaatacc 1800

attatttcta agaaatcact tcgtgatatt attagtgatg taattaaggt atgtggtgtg 1860

gctaaggctg ccgacttcct ggatggaatc aagaacttag gttatcagat ggcgttcaag 1920

ggtggtttgt cattcaactt gggtgatatt atcattccgg aacaaaaaga ggcattggta 1980

cagaaaggat atgatgaagt tgagcaggtt atcaacaact ataacatggg tttcattacc 2040

aataatgaac gttacaatca ggtgatcgat atttggactc atgttaattc agagttgtct 2100

aatatattga tgaagaccat ttcgagtgat gatcaagggt tcaatgctgt atatatgatg 2160

ttggattctg gtgcccgtgg ttctaaagag cagattcgtc agttgtcagg tatgcgtggt 2220

ttgatggcaa aaccgcagaa agcaggtgca gaaggtggcc agattattga gaacccgatc 2280

ttgtcgaact ttaaagaagg actttcggtg ttggagtact ttatctctac ccacggtgcc 2340

cgtaaaggtt tggcagatac ggcgttgaaa acggctgatg ccggatattt gactcgtcgt 2400

ctggttgacg tatctcatga tgtgattatt aatgaagaag actgcggtac gctccgtgga 2460

ttggtttgta cagacttgaa gaataatgat gaaatcattg ctacattgta tgagcgtatc 2520

cttggccgtg tatctgtaca tgatattgtt catcctacaa caggtgaatt gctggttgca 2580

ggtggtgaag aaataaccga agaagttgct aagaagattc aggattctcc gattgaaagt 2640

gttgaaatac gttctgtatt gacttgtgaa tcgaagaagg gtgtttgtgc aaaatgttat 2700

gggcgtaatc ttgcaacgag ccagatggtt cagagaggtg aggctgttgg tgtaattgct 2760

gcacagtcta tcggtgagcc gggtacgcag ttgacgttgc gtactttcca cgccggtggt 2820

acggctgcca atattgcagc caatgcaagt atcacagcta agaatccggc acgtctggaa 2880

tttgaagaac ttcgtactgt agatgtggta gatgaagcag gcgaaccggc aaaggttgta 2940

gtaggtcgtt tggctgaagt acgctttgtg gatgtgaata ctggtattgt tctttctact 3000

cataatgtac cttatggttc tactctttat gcagctgatg gtgaggtcgt agaaaaaggt 3060

aagatgattg cccgctggga tccgttcaac gctgttatta taacagaagc tacaggtagg 3120

attgagtttg aaggtgttat tgagaatgtt acctataaga tagaatctga tgagtctacc 3180

ggtctgcgcg aaattatcat catcgagtct aaggataaga cgaaagttcc gacagctcac 3240

attatgacag aagatggtga cttgattcgt acatataacc tccctgtggg tggtcacgtt 3300

gttgtcgaaa acaagcaaaa ggtaaaagcc ggtgaagtta tcgtgaaaat acctcgtgca 3360

gttggtaagg ctggtgatat cacgggtggt cttccacgtg taacagagtt gtttgaagct 3420

cgtaatccgt ctaatcctgc tgttgtttct gaaatcgatg gtgaggttac aatgggtaag 3480

gttaaacgtg gtaatcgtga aatagttgta acttctaaga ctggtgaagt taagaagtat 3540

ctggtaccgt tgtcaaaaca gattctggta caagagaacg actatgtacg cgctggaact 3600

ccattgtctg acggtgctac aactcctgct gatattcttg ctattaaagg tcctacggct 3660

gtgcaggaat atatcgtaaa tgaggtacag gatgtatatc gtctgcaggg tgtgaagatt 3720

aatgataagc actttgaaat tatagttcgc cagatgatgc gtaaagttga aatcgatgaa 3780

ccgggcgata ctcgcttcct ggaacaacag gtcgtggata agttggagtt catggaagaa 3840

aatgatcgta tttggggtaa gaaggtggtt gttgatgctg gtgattctca gaatttgcag 3900

ccgggacaga ttgttactgc tcgcaaatta cgtgatgaaa acagtatgct aaaacgccgt 3960

gacttgaaac cagttgaagt gcgtgatgct gttcctgcaa cttctactca gattcttcag 4020

ggtataactc gtgctgcttt gcaaacttca agcttcatgt cagctgcttc attccaggaa 4080

actacaaagg ttttgaacga agctgctatt aatggtaaga ttgataagtt ggaaggtatg 4140

aaagagaacg ttatctgcgg tcacttgata cctgccggta ctggtcaacg tgaatatgaa 4200

aagataattg ttggctctaa agaggaatat gatcgtattc ttgcaaataa gaaaacggtt 4260

cttgattata actcaatgga tgttgaagaa taa 4293

<210> 28

<211> 1221

<212> DNA

<213> Ruminococcus obeum

<220>

<223> isolation from intestinal tract, Ruminococcus obeum

<400> 28

atgaaaaaaa ataatttctt tacaagcctg cctttcaaac tgttagtcgg tgttatcatc 60

ggcatcattg caggtatcat tttaaacaaa acggacggtg cttcactgac tgttgcgatt 120

ctgaatatca ttgttacagt gaaatatgtt ctcggtcagc tgatcaactt ctgtgtacca 180

ctgatcatta tcggttttat cgcaccatct atcacaaagc tcggcaacag tgcctcccgt 240

atgctgggtg ttgctcttgt gatcgcttat gtatcttcta tcggtgcagc tcttttttcc 300

acaggtgcag gatatatact tatcccacat ctttccattg ttactgatgt agaagggctt 360

aagacacttc cggaagtcgt atttgaactt tccattccac agatcatgtc cgttatgagt 420

gctctggtat tttctatcat ggtaggtctg gctgctacat ggaatcagac aaagctgatc 480

accggtgtac tggatgaatt ccagaaaatc gtgctttcca tcgtatccaa gatcattatt 540

ccgattcttc cgttctttat cgcacttact ttctgcggac ttgcttacga aggatctatt 600

acccaacagc ttcctgtatt ccttcaggtt atcgtaatcg tactgattgg tcactttatc 660

tggatggcac tgctctattt tcttgctggc gcatattcac atgaaaaccc gatggaagtt 720

gttcgccact atggaccggc ttatctgact gcagtaggaa ccatgtcctc tgctgctact 780

cttgcagtcg ctcttcagtg tgcaggcaaa gcaaaaccac ttcgtaaaga tatggtacag 840

tttggtattc ctctttttgc caacatccat ctctgcggat ctgttcttac tgaagtattt 900

tttgttatga ctatttcaaa gatcctgtat ggctctatcc cggcacctgg caccatgatt 960

cttttctgcc tgctgctcgg aatcttcgca ctcggagcac ctggtgttcc gggaggaact 1020

gttatggcat ccctcggact gattaccggt gttctcggtt tcaacgaaac aggtactgct 1080

cttatgctga cgatctttgc ccttcaggac agctttggta cagcatgtaa cgttaccgga 1140

gatggtgctc tcacccttat cctcaccgga tacgccaaga gacacaatat taaagaacag 1200

aatatcagcg tagatttata a 1221

<210> 29

<211> 237

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 29

accgaagaac ctgctttcat ccggacgtac actggcagta cacatgtatg tactttccca 60

ggaaggtctg catacggatc aggcatttgc caccgcagtt attttgctgg taatggtact 120

tctgatcaac attttatctt ccgttatcgc aaagaaaatg cagagggact aaagtattgg 180

aatcttgatt acgaaacaaa agtgacagga aacgtatttg caaacagcaa tatgtga 237

<210> 30

<211> 1764

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 30

gtgaaacact ttatttctcg ccgcaatttc ctgaaggccg ccggtgtcac cgccgctgcc 60

tcggccatgg ccgctgccgt cccgcaggct tccgccggct ggctgacggg caaggacgcc 120

gccgtcacca tcctgtacac caacgacgta cacacctaca ttgataacaa gtcccccaag 180

ctgacctatg ccgccatcgc cgccctgaag cagggctatg ttgatgaggg caagcccgtc 240

ctgctggtgg acgcaggcga ccacattcag ggcaccgcct acggctcgat ggacgacggc 300

gcgaccatca tcgagctgat gaacgaggcg ggctacgatg tggccacgct gggcaaccac 360

gagttcgact acggcatggc ccgcgccaag gccgtcctca aggaagcgga cttcccctat 420

gtctcctgca actgggtgga cctgcgcacc ggtctgcgcg tcctgcctgc cgtcaagctg 480

ttccccgcag gcggcaagtg gatcgcattc gtgggcatca ccacccccga gtcctttacc 540

aaatccaccc cggcctactt catgaacgcc aagcagacca agtacatcta cgacattctg 600

ggcggtgacg acggcaagaa gctgtacgac gccgtgcaga agggcatcga caaggccaag 660

gcactgggcg cagacgtcat catcggtctg ggccatctgg gcgttgaccc ctcctcctct 720

ccgtggacca gcgaagaggt catcgccaac accaccggct tcgacgcctt catcgacggc 780

cactcccaca ccgtcatgga gaacaagcag gtctacgacg ccgccggtaa ggccgttacc 840

ctgacccaga ccggctccta cctcgccaat gtcggcaaga tgaccctcgc cgaggacggc 900

accatcacca ccgagctgat ctccaccgca gacgtctccg acgccgccgt ggccgctacc 960

gccgcaacgt ggatcaagga agtggacgag atgctgggcg agcagatcgc cgtcaccgac 1020

atcaacttct acatcagcga ccccgccacc ggcaagcgcc gcatccgctc gggcgagacc 1080

aacctcggcg actttgtggc cgacggcatc tacacctact tcaacgagat cgagcagctg 1140

gactgcgaca tcgccatgat gaacggcggc ggcatccgca ccgacgtgag cgcgggcgcc 1200

tggtccttca agacctgcaa gcagatcagc cccttcggca acgtggcctg cctgatggcc 1260

gtcaccggcc agcagatcca ggatgcgctg gagttcggtg cccgcttcgt cggcccggag 1320

ggcaaggaga acggcggctt cctgcacgtc gccggtgcga cctatgagat ccacaccgac 1380

atccccaaca ccgtccgcac cgacgacaag aacgtctggc agggcagcgc caccggcacg 1440

ccccgcgtcc agaacgtgcg catctacgac cgaaagaccg gcacctatga gccgctggac 1500

ctgaaccgca cctacgcgct ggccggtatg aactacaccc tgcgcaatct gggcgacggc 1560

ttcgccatgt tcgacggcgc agagctgatc aaggactatg tcgcggagga ctacctcgtc 1620

atgtccacct acgccatgat ctttgacggc gcgaccgagg gtatgccggt tctggcaagc 1680

gccaacagcc cgctggcaag ctaccccggc tacctgctgg attatgagaa cccgtatggc 1740

gcaggacgca ttcaggtcgt ctga 1764

<210> 31

<211> 1893

<212> DNA

<213> Dorea longicatena

<220>

<223> Dorea longicatena isolated from intestinal tract

<400> 31

atgaataagg gaaaacgtcc aattgggaag atccttatga tactgtcggt tgtattcttc 60

taccttccga tcctgtacat gattatattc tcatttaacg atggaaaatc acttacaagc 120

tttaccggat tttcgctgcg ctggtataag catatgctgg aatcggcaga tatgatggat 180

gcactgtcga cgacattcag tatcgcgctg cttgcgacat ttatctcaac agtagcggga 240

acaattgcag cgatcggagt cagcaagtcg aagaaactga tacgtgacct gatggagcag 300

gcgaataacc tgccgatcat gaacccggag atcgtaactg cgatcggatt catgttatta 360

ttcatcacat tccatgtgga aaaaggatac gttaccatgc ttctggcaca tattgcattc 420

tgtataccgt atgttatgtt atcggtaatg ccgaagatca agtcacttga tccgaacctc 480

gcagatgcgg cgatggactt aggtgcaaca ccgtggcagg cactgacaaa agttgtcgtg 540

ccgcagatca cgccgggaat cgtagccggg gcgctgatcg catttaccat gtcggtggat 600

gatttcatca tctcttactt tgtaaccgga cgtggtgtga agaacttaag tatcatggtg 660

tatacaatga gtaaacgtgt caacccgagt atcaatgcaa tctcgacact ggtggttgtg 720

atcatcacga tcgtacttgt gatcatcaat gctgcaccgg cactttttgc aaaacgtgcg 780

aagagaaaca gcatcggcag acgaaatgta ctgttaccgg cagttgcggt tgcgtgtgta 840

ctggcgatcg tagccggagt tgtgacatat aataacaaga aagagccatt gccattcgag 900

gggcagacgc ttcgtatcta caactggggc gagtatgtag gtgaacatat tatccaggat 960

ttccagaaag agacaggggc aacggtactt cttgaaaatt ttgattcgaa tgaacagatg 1020

tatattaagg ttgcaaatgg agaagcttac gacattcttg taccaagtga ttatatgatc 1080

cagagactca tttccgaagg atatttacag aaattggata agtcgaaact gaactgtatg 1140

gataaattat atgatgcggt caaaggactt ccgtatgatc cggagaatga atattctgtt 1200

ccgtatttct ggggaacagt cggaatcgtg tacgataaga caaaagtcga catcaaagac 1260

ctggaaaaag aaggctataa cattttcctg gatcagaaat ataaagggga catctatctg 1320

tatgactcag agagagattc ctttatgatg gcattgaagg cattgggata ttcgatgaat 1380

acgtctgacg aaagccagat ccaggaagcg tataactggc tggttcaatg tgtacagacc 1440

atgaagccgg agatcgttac cgatgagatc attgataaca tggcacaggg aaggaaggca 1500

cttggtctga tctattccgg agatgcgaca tatgtgatca atgagaatga aaacatggga 1560

tactacctac cggaaggcgg aaccaaccag tggtccgacg ccatggtcat tccgaagaat 1620

gcgaagaatc cggaacttgc acatgcattt atcaattatg caagtgatta tgacggagct 1680

tatgacaatt caagttatgt aggttataca tcggcgaata agaaggttat gaaagatctg 1740

tatggcaagg gcggagagtt cgaaggaatt gatgcatata taccaagaac agataataag 1800

aatgatgaag tctttgagta taatgaggag acgaagaaag agatcagtaa tctttggagt 1860

aaggtgaaga ttgcggctag taatacgaat taa 1893

<210> 32

<211> 996

<212> DNA

<213> Veillonella sp.

<220>

<223> isolated from the intestinal tract, Veillonella sp.

<400> 32

atgactatga tgaataaacc tgtagggatc attggtactg ggagcttcct tcctgacaat 60

gtagtaacaa actttgatct tgaaaaaatg gttgacacta acgaccaatg gattagagaa 120

cgtactggta tagaagaacg acgcattgca ccggaaggta tgaacacatc ctatatggca 180

acagaggcag ctaagaaagc attgcaaatg gctaaggtca atgcagaaga catcgatatg 240

attatctttg ctaccttaac accagatatg attattcctt ctgcagcttg tattttacaa 300

gctaacttag gagctaaaaa agcagcagct tacgatttac aagcggcatg ttctggcttt 360

gtatatggac tgattactgc agctagttat attagttctg gcatttataa gaaagtactc 420

gttgtagggg cagaaattct ttcccgtcga gtaaattgga atgatcgtgg cacatgtatc 480

ctcttcggtg atggtgcagg tgctgcagta gtatctgaag tacctgaagg ttacggtatt 540

aaaggtattg atatgggcgc cgacggcact ggtggaccag cactttgtat tccagcaggt 600

ggtactgctg tagttgctaa tgatcaacgc gtagaagaag gcttgacatt cattcacatg 660

gatggctctg aagtatataa atttgctgtt aaaacgatgg gacgtactgt tttaaaatct 720

ttagaacgtg ctggaatgga gctcaatgaa ttagattact tcattcctca tcaagcaaat 780

attcggatta ttgattcggc agcaaaacga ttacatctgc cgatggaaaa agtttttgtt 840

aatttacata agtatggtaa tacatctgcc gcgtctgtag cgattgctct tgatgaagca 900

aatcgtgaag gacatttcaa acgtggagat aatattgcgt ttgcaggatt tggtgcgggt 960

ctaacatggg caagcctagt cttgaaatgg tattaa 996

<210> 33

<211> 339

<212> DNA

<213> Eubacterium hallii

<220>

<223> isolated from the intestinal tract, Eubacterium villii

<400> 33

atgtcttttt ttgaagtgtt ttcagcatac tacaacaata attcaagtat cagtccttca 60

aaaaacaact ccacagaaag ccacatcctt ctggagccta ttcaaatccc tccagcaata 120

aatagaattt ccctccccag ccaaagcaca gcccccgaag agagtctagc cccctatatt 180

tgtggcctag actctcttcg ggggcgtctc tttgcctgcg gcaaattcat atttactctc 240

catagaagtg gaagccttcc tccctctgtt ttctccgaag agatcgtcgg atttctgccc 300

gctcccactc ttttcggtct tcttccgatt ctaaaataa 339

<210> 34

<211> 1017

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 34

atgaatgtta atgaaataag agaaaaaagt agaagtgtta ttggacctta ttgtaagtca 60

tgtccagtat gtaatggaaa agcttgttcg aataaaatcc ctggaccagg ggctaaggga 120

acaggaacag ttgctattaa aaattatgat gcttggcaaa agatagaagt taaaatggat 180

actcttgctg aaaataaaga aattgataca acctttaaaa tgtttggaaa aacatttaag 240

tatcctattt ttgctggacc agtaggagca gtaagtcaac attatagtga tttatataat 300

gataatatgt ttaatgaaat ttatgtacgt gcttgtgcaa aaagtgggat tgctgctttt 360

acaggggatg gtttaaatga agaaatcatg atgcatgcaa cacattatat taaagaaaat 420

aatggcattg gtgttccaac gattaaacct tgggataaag atacttgctt taaaaaatta 480

gatcttgcta atgaatcaaa tgcttttgcg attgctatgg atattgatgc tgcaggactt 540

ccttttttac aaggaagaat tcctccggct ggtagaaaga gtgtagaaga attaaaagag 600

attattgatt atgcccacgt tccttttatt gttaaaggga ttatgtcagt tgaaggtgca 660

caaaaagcgg tagatgcagg tgccaaagcg attgttgttt ccaatcatgg aggaagagtt 720

ttagatggga ctcctgcaac agcatgtgtt ttagaagaaa ttgctgatca atttaaagga 780

caagttaaaa ttattgttga tggtggtatt cgtagtggtt tagatgtttt taaagccctt 840

gctttaggtg cggatgcagt cattatagca agaccatttg ttaatatgat ttatggtggt 900

aaagatgaag gtgttgaagc ttatgttcaa cagttaggtc aagaattaga aaatacgatg 960

atgatgacag gtgtttcttc tttaagagaa atttctaaaa agaatattta taaatag 1017

<210> 35

<211> 2211

<212> DNA

<213> Coprococcus sp.

<220>

<223> Coprococcus sp, isolated from the intestinal tract.

<400> 35

atgttaaatc cgggaacgat gctcagcggt cgttatgaga tcatagaatt ggttggatct 60

ggcggtatgt cagaggtata taaggcaaaa tgtcatgtgt taaacagata tgttgccata 120

aaggtgctta agcctgagtt cagctcagac gttaattttg ttacaaagtt caggatagag 180

gcccagtctg cggcggggct gtcacatcct aatattgtga atgtatacga tgtcggtgag 240

gacaacggaa tatattatat agtaatggag cttgtggagg gaaccaccct caaggaatat 300

atacaggccc acggtagaat agagcctatg caggcgattg attttgccat acagatagcc 360

cagggacttc aggcagccca tgagcatcac acgatacaca gagatataaa gcctcagaac 420

atcatcatgt cgaacaacgg cacgctgaag gttactgatt tcggaatcgc aagagccgca 480

tcgtcaagca cgaccacgac aaatgcgatg ggcagtgttc actacatatc accggagcag 540

gcaaggggcg gattctcaga tgagagaagt gatatttatt cactcggtat caccatgtat 600

gagatgctca caggacatgt tccatttgaa ggcgagaaca atgtggctat agcgctgatg 660

cacattcagg gcgagatggt atcacctagg gagtattacc cggatatacc tacaagcctt 720

gagaagatca taagaaagtg tacacagaag aagccggaga gacgatatct cacagcaaat 780

gcgcttatag ctgatctcaa cagagtaaaa gagaatccta atatagattg tatagtagtg 840

cctaagacca gcgttcctac atcccctacc attgagatga caaagcagga gatggagatg 900

atcaagaccg gaagacaggt cgataaggca gatactacgg aggttccacc tgtaagtccg 960

agaacgtcag agatcaaggc taacgtgcca gttatgccgc cgtcacagtt cgacgatctg 1020

ttcccagatg acgacgatga agaggatgat acggacgagt cagtggcaga tgatggagaa 1080

caggagcagg aatacgatgg agaagaggag cttgatccaa gacttaagaa gataatcatg 1140

gtatcaagta ttgccatcgc ggtggttctt gccatacttg ccatacttct cataggcaag 1200

atagccggct ggaatattct gggcggtaag ggtaagacgg aggaaagcac cgatgtcata 1260

accatagagg gatcgaacga cacaactgca caggagacag tacctatggt caatgtagta 1320

ggtctgtata agactgcggc tgaggagcag atgaagaaga atggcttcac caactataca 1380

tttgtggaga agacagatgc cacagttgag aagggctatg taatatcaca gagtatagat 1440

gatggaacat ccataacaaa ggatacacag ataactattt acgtgtcatc tggcaaggaa 1500

aagaccaagg tgccgaatgt tctcggatat gaggacagtc aggccacaac attgctcgag 1560

gaggcaggac ttaaggtaac acatggctat gcgtacgatg ataatgttga gaaggatcat 1620

gtaatatcct cagatcctgt tgccggaaca gaggttgagg agggctcaac agtcaagata 1680

atcatcagta acggtaagga gactaagaag atcgcagttc ctaacctcaa gggtaggtct 1740

gaggcagatg cagcagctaa gcttcaggag gtcaacctgg taggcaatcc aagctatgag 1800

tacagcgatg atgtgaagga aggccaggta atatcacaag atcctgaggt caacactgag 1860

gttgacgagg gaactaccgt gtcatatgtt gtcagtaagg gacctcagaa ggttacatac 1920

tctgtatctt tcagcggttc aatatcgaat atagagtttg attttgatac atttggaagt 1980

gtgacagttg acatctcata cacaataggc agacagacat tctctgtata cagcggcaag 2040

gctggtgcag gagacttccc tatcaatata gatggagttg agggtcctac aggacttgag 2100

agcaaccagg gatcattctc tgtgaccatc acggattcag agggaataga tgtcacatca 2160

agctttaaca cgggcggttt gtccgctaca tttacacagg agagtaatta g 2211

<210> 36

<211> 930

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 36

ttggaaagat acggttataa tgttattata ttaacaatat ttttaaaaca aatggaggat 60

ctcattatgt tagtttcagc aaaagaaatg cttgaaaaag caaaagcagg tcactatgca 120

gttggtcagt tcaatatcaa taaccttgag tggacaaaag caatcctgtt aaccgctcag 180

gagaataatt ccccggttat tcttggtgtt tccgaaggtg ccggcaaata catgactggt 240

tacaaaacgg ttgttggtat ggtaaatggt atgctggaag agttaaatat cactgttccg 300

gttgcattac atctggatca cggcagctat gatgcctgct acaaatgtat cgaagctggt 360

ttttcttctg taatgtttga tggttctcat tatccgatcg aagagaatgt tgcaaaaaca 420

aaagaacttg ttgctgtatg caaagagaaa ggattatctc ttgaggcaga agttggttcc 480

atcggcggtg aggaagacgg cgttgtcgga atgggcgaat gtgcagatcc ggatgagtgt 540

aaatctatcg ctgatcttgg agtaacaatg cttgcagctg gtatcggtaa tattcatggt 600

aaatatccgg aaaactgggc aggattaagc tttgagactc tggatgcgat ccagcagaag 660

accggtgata tgccattagt tcttcacggt ggtacaggta ttccggatga tatgatcaag 720

aaagcaatct ctctcggtgt tgcaaaaatc aacgtaaata cagagtgcca gcttgtattt 780

gctgaagcta caagaaaata tatcgaagag ggcaaagacc tggcaggtaa gggattcgat 840

cctagaaaat tacttgctcc tggttttgaa gcaatcaaag caaaagtaaa agagaaaatg 900

gaaattttcg gttctattga caaagcctga 930

<210> 37

<211> 522

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 37

gccgatgccg gagggcgtgg taccatagcg cgaacgcaaa atcggaaaag tgaggcccag 60

atggtcgatc aggaaaaagt cgaggccgcc attaagctgt tgcttgaggg cataggcgag 120

gacccaactc gtgagggcct gctggagacc ccggcgcgcg ttgcccgtat gtatggcgag 180

atcgccggcg gtatggacca gagtgccgag gagcacctgt ccaaaacctt tgccgtcgct 240

tcgaacgatt tggttatcga gcgcgacatc acgttttact cgctgtgcga gcatcatctg 300

ctgccgtttt acggcaaggc tgccattggc tatatcccca acggccgtgt cgcagggctt 360

tctaagcttg cccgcacggt agaggtctac gcccgtcgtc tgcagctgca ggagcagttg 420

tgcgcacaag ttgccgatgc cctcatggag tatctcgctc cccagggagc gattgtgctc 480

atggaagctg agcatatgtg catgaccatg cgcggcgtgc aa 522

<210> 38

<211> 2826

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 38

atgaagaaac ttattgcaat gacactactg ctcggaggca gtactctgct ctgcgcacaa 60

aagaccgtga agataccggc tgtgtacaaa cccgtcaaaa acgagatgta caaaaaaggc 120

tggattgatt tcaacaaaaa cggagtgaaa gatgtgtacg aagaccctaa tgccacgctg 180

gatgcacgta tcgaagatct actcagccag atgacactgg aagaaaagac atgccagatg 240

gtaaccctct acggttacaa acgtgtgcta aaggacgacc tccccacccc cgaatggaaa 300

cagatgcttt ggaaagacgg catcggtgcc atcgacgaac acttgaacgg ttttcagcaa 360

tggggacttc ccccatcgga taatccctat gtatggccgg catcccgcca tgcctgggca 420

ctgaacgaag tgcaacgctt cttcatcgaa gagacgcgtc tcggcatccc cgtagatttt 480

acgaacgaag gtatccgagg agtagaaagt taccgtgcca ccaatttccc cacgcaactg 540

ggattgggac atacctggaa ccgcgaactg atacgccaga ttggactaat caccggacgg 600

gaggcccgta tgctgggata taccaatgtg tatgccccca tcctcgacgt cggtcgtgac 660

cagcgctggg gacgctatga agaagtatat ggtgaatctc cttacctcgt tgccgaactg 720

ggtattgaaa tggtacacgg catgcaacat aaccatcagg tagcagcaac cggaaaacat 780

ttcgttgctt acagtaacaa caagggcgcc cgcgaaggta tggcacgtgt agacccgcaa 840

atgtcaccgc gcgaagtgga aatgatacat gtctacccct tcaaacgggt gataaaggaa 900

gcaggactat tgggagtgat gagttcgtac aatgattacg acggtgtccc catacaaggc 960

agttattatt ggctgactac acgcctgcgt ggcgagatgg gattccgggg gtatgtggtt 1020

tcggacagtg atgccgttga atatctctat accaaacaca gcaccgccaa agacatgaaa 1080

gaagccgtgc gccaaagtgt agaagccggc ctgaatgtac gttgcacctt ccggtcaccg 1140

gattcatatg tattgccttt gcgcgaattg gtaaaggagg gcggactaag cgaagaagtt 1200

attaacgacc gtgtacgtga cattctgcgt gtaaaattcc tcgtagggct gttcgacgct 1260

ccctatcaga ctgaccttgc aggagctgat aaagaagtag agaaagctga gaatgaagcc 1320

tttgcactgc aagcctcacg cgagagcctc gtcttgttga agaatgaaaa caacgtgttg 1380

ccattggata tcaacaacgt gaagaaaata gcagtctgcg gccccaatgc ggacgaagag 1440

ggatatgcac tgacacacta cggtcctttg gcagtagaag taaccacagt gctggaaggc 1500

attcgtcaaa aatccgaagg taaagcggaa gtgctctaca ccaaaggctg tgaccttgta 1560

gatgccaatt ggccggaaag cgaacttatc gactatccga tgacagatag cgaacaagct 1620

gaaatcaata aagcagtaga aaacgcacgc caggcagatg tagccgtagt ggtattagga 1680

ggcggacaac gcacctgcgg tgagaataag tcacgcagca gtctcgacct tccgggacgg 1740

caactgaaac ttctccaagc tgtgcaggca acaggtaagc ccgtcgtgct ggtattgatt 1800

aacggccgtc cgctttccat caattgggcg gataagtttg ttcccgccat tctggaaacc 1860

tggtatccgg gctctaaagg tggtacggca gtggccgatg tactcttcgg agattacaat 1920

ccgggcggaa agctgactgt taccttccct aaaagtgtgg gacaaatccc tttcaatttc 1980

ccttgcaaac cttcttcgca gattgacgga ggaaagaatc cgggaccgga tggaaatatg 2040

tcacgtgtca acggtgcact ttatcccttc ggttacggtc tgagttatac caccttcgag 2100

tattccgata ttgagatcag cccgaaagtg ataactgcca atcagaaagc aaccgtgcgc 2160

tgcaaagtta ccaataccgg aaagcgggta ggcgatgaag tagtgcaact ctatatccgc 2220

gatatgttaa gcagcgtcac cacttacgaa aagaaccttg cagggttcga gcgtgtccat 2280

ctgcaaccgg gagagacgaa agaaatcacc ttcaccctcg accgcaaaca cctggaacta 2340

ctggacaaac acatggaatg ggtggtagag cccggagact tcagcgtcat ggtaggcgcc 2400

tcttccgaag acatccgcct gaatggaatg ttgacagtag aagacccgaa cgctcctgta 2460

caaacgcaag cccaaacaga cagtcccgtg actgccagca ccaatccgga aagcgtaatg 2520

aacgtacttg ataaaaaaat gaatactgtt tgggagggaa ataaaggaga ttatattacc 2580

tttgccttag agaacggctc caaggtagat ggtgtttcca ttgcattcag cagaagcaac 2640

ggtctgccgg cagagtttga gatacagctt tcgggcggtg gcggacagtt cctgacggtt 2700

tattcgggca cagtcagcga gtacggcaag ctgatttcat acacgttcaa gggaactact 2760

gccagcgact tgcgcatcgt gctgaacgat gaccgcgtag gggtggcgga agtgaaactg 2820

gaataa 2826

<210> 39

<211> 1224

<212> DNA

<213> Eubacterium hallii

<220>

<223> isolated from the intestinal tract, Eubacterium villii

<400> 39

atgcaagaga gaaatttaaa ggtaatagaa ggtttattgt ttgtcctttt tattttgtta 60

tggggcttat ttttatcggg aggaagtgga aaggtaaagc ggagaacact ctctttatgg 120

caatatattc cagtgcacac ggaggtgttt catgattttt ttgaaaatag tataagaaaa 180

gggcagtatg aattgacgcc aattcttgga tacgatacat acgagggggg aaagggaatg 240

ctccttcctt tttttcgaaa tggaatgcag gaggagcttt tcccaattca aagatttgcg 300

cttgattact ggaaaggaaa ggggtactat aaggcaaaat acagtgatac ggtgccggat 360

tattttacag aatcggatga tcagtctgta gaagaaaaga aaaaagatcc tggaaaaatg 420

aagcagatgg gaacgggaag agcctataca ataaaagaac tggcaagtta ttcttttttg 480

ctggaacatt tttatattgt ggatgagaca acatctatga caaaacagga tttaaatgga 540

acaaagcttg tgacgatgga tttgtctgca aaattaggaa aggaggagcc actggttctt 600

atttaccata cacacggaag tgaaacgtac aaaaaggtaa atggaaaaga aggttctgtg 660

attgaagtag gaactgctct gacaaaagaa ctggaaaatg tctacggaat aaaaacggta 720

catgacaaat ctgtttatga tatggtagac ggacagcttg accgcaatgc cgcttataac 780

tttgcaggtg atagtgtgga ggcggcactt aggaaaaatc cttcggtaaa agtagtgatt 840

gacctgcaca gggattctgt agaaaataat attcaccttc gcacaaaagt caatggaaag 900

tctgcagcac agattatgtt ttttaatgga gtcagccgcc ttgcaagtaa gggggatatc 960

ggttatcttt ataatccaaa caaggaggca aatctggcat tcagcctgca gatgcagctt 1020

ttatgtggaa aatattatcc agatctgaca cgaagaatct atataaaagg ctaccgttat 1080

aaccttcatt tagcaaaaag agcgatgctt gtggaggtag gagcacagaa caatacagta 1140

gaggaagcga aaaatgcaat gcaaccatta gctgagatgc tatatcggct cttatctggg 1200

gaaaaatctt ataaaaagaa ttaa 1224

<210> 40

<211> 702

<212> DNA

<213> Klebsiella oxytoca

<220>

<223> Klebsiella oxytoca isolated from intestinal tract

<400> 40

atgaataaga tcctgctgca atgcgacaac ctgtgcaaac gctatcagga aggcagcgtg 60

caaaccgacg tgttgcatga tgtcagcttc agcattgaag agggcgagat gatggcgatt 120

gtcggcacct ccggttccgg caaaagtacg ctgctgcatc tgcttggcgg gctggatacg 180

ccgacctccg gcgacgttat cttttccggt cagtcgatga gccgactctc agccgccgcg 240

cgcgccgatc tgcgcaaccg cgagctgggc tttatttatc agtttcacca cctgctgccg 300

gatttcaccg cgctggaaaa cgtggcgatg ccgctgttaa tcggtaaaca gaaggcggcg 360

gatattgagc gtcaggcgaa agcgatgctg cgcgccgtcg ggcttgagca ccgcagcgac 420

caccgcccgt cggagctctc cggcggcgag cgtcagcgcg tagcgattgc ccgcgcgctg 480

gtcaataaac cgcggctggt gctggcggat gaacctaccg gtaaccttga tgcccgcaac 540

gccgacagca tttttcagct gcttggcgaa ctgaacgtgg cgcagcgcac cgcctttctg 600

gtggtgaccc acgacctgca gctggcaaaa cggatgagcc gccagctgga gatgcgcgac 660

gggcgcctga ccgccgaact ctccctgatg ggggctgagt aa 702

<210> 41

<211> 2487

<212> DNA

<213> Bacteroides xylanisolvens

<220>

<223> isolated from the intestinal tract, bacteriodes xylanisolvens

<400> 41

aaaagcaatt ggaaggaaat gaataaaaag agattaatcg gatatctgtt cgtcacgatg 60

tctgttggtt gtattcaggc gcaggaacag aagtcttctg tacccgaata caagctatgg 120

tacgactgtc ccgcacaggt atggaccgag gcattacccc ttgggaatgg tcgtttgggg 180

gccatggtat atggaacacc aggcacagaa caaatacaac taaacgaaga aagcatttgg 240

gcgggacgtc ccaataataa cgccaatccg aatgcactgg aatatattcc gaaagtgcgt 300

gagctggtct ttgccggaaa atatcttgaa gcacagacac tggccacaga aaaggtgatg 360

gcaaagacta attcaggaat gccataccaa agttttggtg acctgcgaat cgcttttcca 420

ggtcatacac gttattccaa ttattacagg gaattaagtc tggattccgc acgtgcaatc 480

gtacgttatg aagtagacgg agtgcagtat caacgcgaaa cgattacttc atttaccgat 540

caggtagtaa tggtacgact gaccgccaat cgtcccggac aaattacttt caatgcacaa 600

ctcacttccc ctcaccagga tgttatgatc gcttctgaag agggaaactg tgtgacactc 660

tcgggagtct cttccttgca tgaaggatta aaagggaaag tagaatttca aggcagattg 720

actgctaaaa ataaaggtgg gaaaatagct tgtgctgatg gaatactttc tgttgagaaa 780

gccgacgaag cggttatcta tgtatccatt gcaactaatt tcaacaacta tcaagatatc 840

accggtaatc agacagagcg ggcaaagaac tatttggcga aagcaatggt tcatccgttc 900

attgaatcga agaagaatca tgttgatttt taccgacaat atctgacacg cgtttctctt 960

gatttgggac gagaccaata tgcgaatgta accaccgata agcgtgtgga gaatttcaaa 1020

aacaccaatg acacacatct ggttgccacc tattttcaat tcggacgtta tttgttgatc 1080

tgttcttccc aaccgggagg acaacccgcc aatttacaag gaatttggaa tgacaaatta 1140

ttcccttcct gggatagcaa atatacttgt aacatcaatt tggaaatgaa ttactggcct 1200

tcggaagtaa cgaatctaag tgaactgaat gaaccgttat tccgtctgat aaaagaagtg 1260

agtgacacag gtaaagagac ggctaagatt atgtatggag ccaacggatg ggtgttgcat 1320

cataacacgg atatatggcg tattacagga gcagttgaca aagctccttc gggtatgtgg 1380

cccagtggtg gcgcatggct ttgccgacat ctttgggagc gttatctata caccggagat 1440

gtggaattcc tgcgttctgt ctatccgata ctgaaagagt ccggacgttt ctttgatgaa 1500

attatggtga aagaacccgt acacaactgg ctggtagtct gccccagtaa ttcaccggag 1560

aatgtacatt cgggaagtaa tgggaaagct actacagctg ccggatgcac catggacaac 1620

caattgattt tcgatctttg gacagcaatt atatctgctt ctcaaatact ggatacagat 1680

caggagtttg cctctcatct gacacaacgt ctcaaagaaa tggctcccat gcaggtggga 1740

cattgggggc aattacaaga gtggatgttc gactgggatg atccgaagga cgtacaccgt 1800

cacatttctc atttgtacgg actcttcccc agcaatcaga tttcccctta ccgtactcct 1860

gaattatttg atgcagcacg tacttctctg attcaccgtg gcgacccgtc gaccgggtgg 1920

agcatgggat ggaaagtttg cctttgggca cgcctgcttg atggtgacca cgcctacaag 1980

ctgataactg accagctgac tctcgtacgc aatgaaaaga aaaaaggaag cacctaccct 2040

aacctgttcg atgcgcaccc accatttcag attgatggaa actttggttg tgccgcaggc 2100

attgcagaga tgttgatgca aagctacgac ggttttatat atttgcttcc cgcactgcct 2160

accgtttgga cagaaggttc tatcaaagga attattgccc gtggaggttt cgaacttgat 2220

ttgagttgga aaaacgggaa agtcagccga ctggttgtca aatctcataa gggaggaaat 2280

tgtcgtttac gttcgctcaa cccattaacc gggaatggct tgaagcgtgc caaaggtgaa 2340

aatccgaacc ctctttatgc agtccccacc attccggagc cattgattaa cgaaaaggcc 2400

aacttgaaca aagtggaaat tgcagagaca tacctctatg acctacctac caaagcagga 2460

aaagaatatg tattgattgg aaaataa 2487

<210> 42

<211> 2385

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 42

atgcaggaca tatataaagt agaaacacat cctctggcgt taagtgaaaa tatgatcaca 60

ggggataaat acaggattac gtttctcaca gagggattga tccgtctgga gtatgatgag 120

gagggcgtat ttgaggatcg ccccacgcag atggtgtttt ttagggattt tccgaaaacg 180

gactatcgtg tagtgcgtac agaggatgga atcgaggtcc atacgaaaag aatccacctt 240

atttacaatg agaaggaatt taccagctgg ggattaagta ttcaggtaaa aggaaaccta 300

agtgcctatc acagcatctg gcactacgga gaagaaatcc atgatctgaa gggaactgcc 360

aggacactgg atatggtgaa cggagctaca gagctggagc atggtattct ttccaggttc 420

ggatattctc tggtggacga cagcaggtcg caggtgcttt tgccggacgg ctggatcgag 480

gcaagaagaa agggcatcaa ggatctgtac ttcttcggat acggacatga ctacaaagaa 540

gcacttcatg atttttacag actctgcgga aaaacaccga tgcttccaag gtttgctctc 600

ggaaactggt ggagcagata ttataaatat tctgaagaaa gctacaatga gctgatggat 660

aaattccagg aaaaaaatat cccatttacg gttgcagtca ttgatatgga ctggcatgtg 720

accgatgttg atccgaaata cggaagtggc tggaccggat atacctggaa taaagagctc 780

ttccctgacc cgaagcgttt tctggatggt ttacataaaa gaggaatgcg taccacatta 840

aatgtacatc ctgcagacgg cgtgcagggc tgtgaagaaa tgtatgagga catggcaaag 900

gccatgggtg tggattatga aaatgaagat ccggtagtat gcgatccggc aagtccgaaa 960

tttatagaag cctatttcaa atatcttcat catccgagag aggaagaagg tgtggatttc 1020

tggtggatcg actggcagca gggaagcaat tgtaagatcg agggtcttga tcctctttgg 1080

atcttcaatc acttccattt cctggacagt gccagaaacg gaaaacgtcc catgaccttt 1140

tccagatatg caggtcccgg aagccacaga tatccgatag gcttttccgg agatactcat 1200

attacctggg attcccttga ttttcagcca tactttacat ccacagcctc caacatcggt 1260

tatggatggt ggagtcatga tattggtgga catatgcttg gatataagga tgacgagatg 1320

accgcccgct ggacacagta cgggatcttt tctccgatca tgcgtctcca cagctcatgc 1380

agtgacttca acggaaaaga gccgtggcgt ttcaaaaagg agacagaggt tgtcatggaa 1440

gaagctctcc gtgagcgcca cagaatgatg ccgtatcttt atacaatgaa ctacagaagc 1500

taccaggaag atcttcctct ggtggagccg atgtattacg aatatccgga ggcaccggag 1560

gcatatgcag taaaaaatca gtactttttc ggaaatcagc tgatggtagc ggctgtgaca 1620

acaccgagag taaaaggcct taatgtagcg aagacggcag tatggctgcc ggatggggta 1680

tggtatgata tttacacagg tctgcgctat gagggtggac gaatggtgga tatgtaccgt 1740

actctggaca gtatcccggt tcttgcaaaa gcaggcggca ttctggtgat gaccgatgag 1800

atccgtggaa cagaggcgga aaagaatccg gaaagcctga atatccgggt attcccgggt 1860

gctgacggaa gcttcagatt gtatgaggat gacaacgaaa cctgtgctta cgaaaatggt 1920

gcctgtgtat ttacggaaat ggattataag gaaaagaatc agggagtgtt taccattcat 1980

cccgcacagg gaaaaacaga gctgatcccg gcaaagagag cttataccgt ggagttctgt 2040

aattttgcga aaacaggcac agatactgtg aaggttctgg ttaacggagc agagactgag 2100

gctgctgtaa aatatgagga gaagcttcag aagatctgtg tggaagtggc agcagataca 2160

gcggcagagg tgcagatcat tctcgcagga gatgtagcgg ataatcgtat agagaaacgt 2220

atctttgatt tcctgaacca ggcagagatt ggttttgtgc tgaaggacag attatatcag 2280

ctgatcacag ccggaaagaa gctgccggtt cttctgtcgg agcttcagtc tatggaactg 2340

gaaaaggatc tttacggagc actgatggag atcctgactg cgtaa 2385

<210> 43

<211> 2520

<212> DNA

<213> Haemophilus parainfluenzae

<220>

<223> Haemophilus parainfluenzae isolated from intestinal tract

<400> 43

atgactgaag atgtaaaaaa tgcggatgcg aatgcaccga aaaaactaag tattcaacgc 60

agaacaaaaa caaccgtaag cagtacaaca gctggcggta aagcaaaagc ggtacaagtt 120

gaagttcgta aaaaacgcac tgtgccaaca gatgctgcgc gaaaagccgc tgaggcagaa 180

aaattaaaag cacagcaaga agcagagaag aaagcggcag aagaaaaagc acgtttagct 240

gctgaaaaag ctgcagcgga aaaagcgaaa gccgaaaaag cgaaggttga agcagaaaaa 300

gcagcgaaac cagcacaaag tgcggttgaa aataaaccta aatctgttga tccagaaaaa 360

gaaaaacgta aagcggaaga agcagaactt cgtcgcaaag cggaagagct ggctcgtcaa 420

aaggctgaag agcaagcacg taaagcagct gaagatgcta aacgttatgc tgaagcggat 480

acatcaagca atgaaagcgc atcagaagat tatactgatt acaatttaag ctctcgttat 540

gctttagaag cagaagatga agaagaacgc cgtaatgaaa atcgtggtcg cggcaaaaat 600

aaaatggcta aagcgaaaaa agaacgcgat gacgtgaagg gcggtaataa aaatgagcgt 660

gaatctaacc gtaaaaatca aaaagacggt aaattcggta aaggtaaaaa tggtaagaaa 720

ggtgcagcat tacaacaagc cttcacaaaa cctgttcagg ttgtgaaaca ggatgttgtt 780

attggtgaaa caattaccgt tgcggaatta gcaaacaaaa tggcaaccaa agcgactgaa 840

atcatcaaag ctatgatgaa gatgggcgaa atggtaacca ttaaccaagt gcttgaccaa 900

gaaacagcac agttagtggc tgaagaatta ggtcacaaag tgattcttcg caatgaaaat 960

gaacttgaag aagaagtatt aggcgatcgc gatgttaatg ctgaaaaagt aactcgtgca 1020

ccagttgtaa cgatcatggg gcacgttgac catggtaaaa cctcattgct tgactatatt 1080

cgtaaagcga aagttgcagc aggtgaggcg ggtggtatta ctcagcacat cggtgcgtac 1140

cacgtagaaa tggatgacgg caaaatgatc accttcttag atacgccagg acacgccgca 1200

tttacctcaa tgcgtgcacg tggtgcgaaa gcaacagata tcgtcgttct tgttgttgcg 1260

gcagatgatg gtgtgatgcc tcaaaccatt gaagcgattc aacacgcgaa agcagcaggt 1320

gcgcctttag tggttgcagt gaacaaaatt gataaaccag aagcaaatcc agaccgagta 1380

gaacaagaat tacttcaaca cgaagtgatt tctgagaaat tcggtggtga tgtgcaattc 1440

gttcctgttt ctgcgaagaa aggtaccggt gttgatgact tattagatgc catcttgctt 1500

caatcagaag tacttgaatt aacggctgta aaagacggta tggcaagcgg tgtagtgatt 1560

gaatcttacc ttgataaagg tcgtggtccg gttgcgacaa ttcttgttca atctggtact 1620

ttacgtaaag gtgatatcgt actttgcggt tttgaatacg gtcgtgttcg tgcaatgcgc 1680

gatgaaaacg gtaaagaaat cgatgaagcc ggcccatcta ttcctgttga ggtgttaggt 1740

ctttcgggcg tgccagcagc gggtgatgaa gcaactgtag tacgtgatga gaaaaaagca 1800

cgtgaagtag cattacaccg tcaaggtaag ttccgtgaag tgaaacttgc tcgtcagcaa 1860

aaagcgaaac ttgaaaatat gtttagtaat atggctgaag gtgatgttgc tgaactgaat 1920

atcatcgtga aagcagacgt acagggttct gtggaagcga ttattcaatc gttacaagat 1980

ctttctactg atgaagtgaa agtgaaagtt gtgggttctg gtgtcggtgg tatttctgaa 2040

actgatgcaa ccctagcggc agcctctaat gcgattattg ttggctttaa cgtacgtgcg 2100

gacgcttctg ctcgccgtat tatcgaagca gaaaatattg accttcgtta ttactctatc 2160

atttatgaac tattaaatga tgtgaaagca gcgatgagcg gtatgctaca acctgaattc 2220

aaacaagaaa tcattggctt ggctgaagtg cgtgatgtat ttaaacatcc gaaatttggc 2280

gcaatcgcag gttgtatggt gactgaaggt gtagtaaaac gtaacaaccc aatccgcgta 2340

ttacgtgata acgtggtaat ctttgaaggt gaattggaat ctctccgtcg ctttaaagat 2400

gacgttgctg aagtccgtaa tggtatggaa tgtggtatcg gcgttaaaaa ttacaatgac 2460

gtaaaagtcg gcgaccaaat cgaggtgttt gaagtcgttg aaattaaacg ttcaatctaa 2520

<210> 44

<211> 3558

<212> DNA

<213> Eubacterium hallii

<220>

<223> isolated from the intestinal tract, Eubacterium villii

<400> 44

atgcctagag caaaacagtc tatggatgga aataccgcag cagctcacgt agcatatgcg 60

tatacagatg ttgcagctat ctatcctatt acaccatcta gtccaatggc tgattccgtt 120

gaccagtggg cagcagcagg acaggaaaat atttttggta accaggtagt tgttagtgag 180

atgcagtctg aggcaggtgc agcaggtact gttcacggtt ctcttgcagc tggtgctatc 240

acaacaacat ttacagcatc ccagggtctt cttcttatga tccctaacat gtataagatc 300

gcagctgagc agcttccatg tgtatttgat gtatctgcac gtacagttgc tacacagtct 360

ctgaacattt tcggtgacca cagtgacgta tatgcatgtc gtcagacagg atttgctatg 420

ctggcagaaa ctaacccaca ggaagttatg gacttaagcc ctgtagcaca tcttgctgca 480

atcgaaggta aagttccatt catcaacttc ttcgatggtt tccgtacatc tcatgagatt 540

cagaagatcg aaaaatggga ttatgcagac ttaaaagaaa tgtgcaacat ggatgcagtt 600

aaagctttcc gtgagcatgc tcttaaccca gaacacccag ctatgcgtgg ttctcacgaa 660

aacggtgacg ttttcttcca gcacagagaa gcttgtaact ctgtttacga agctttacca 720

gctgttgtta agaaatacat gggcaaaatc aacgaaaaac tgggaactaa ctacgattta 780

ttcaactact atggtgcaga agatgcagac cgcgtaatca tcgctatggg atctatctgt 840

gacgtagcag aagaagtaat tgattactta acagcaaaag gtgaaaaggt tggtttaatc 900

aaagttcgtt tatatcgtcc ttgggtatct tctgcattat tagacgttat tcctaagaca 960

gctaagaaga tcgctgttct tgaccgtaca aaagagcctg gagcattagg agatccttta 1020

tatcttgatg ttgctacaac tcttagagaa gcaggtctta acgatatcgt tcttacagct 1080

ggacgttacg gattaggttc taaagatact cctccttcat ctgtatttgc tatttataaa 1140

gaacttgaaa aagacgctcc aaagagccgt ttcacaattg gtatcgtgga tgatgttaca 1200

aacttatctt taccagaagt taaaccagct cctattacat ctgctcctgg tacagtagag 1260

tgtaagttct ggggtcttgg tggagacggt actgtaggtg ctaacaagaa ctctacaaag 1320

atcatcggtg accacacaga taaatacatt caggcatact tccagtatga ctccaagaag 1380

acaggtggta ttacaatttc tcaccttcgt tttggtgaca atccaatcag aagcccatac 1440

tacattaatc aggctgactt cgtagcatgt cacaacccat cttatgtagt taaaggttac 1500

aagatggttc aggatgttaa accaggcgga atcttcatga tcaactgcca gtggtctgat 1560

gaagaattag atcatcatat gcctgcagca gctaagaagt atattgctga caacaacatt 1620

cagttataca caatcaacgc gattgataag gctattgaga ttggtatggg taaacgtact 1680

aacacaatct tacagtccgc attcttcaaa ttagcgaaca tcatgcctat cgacgaagca 1740

gtagaattca tgaaagcagc agctaagaag tcctacagca agaagggtga cgctgttgta 1800

gagatgaact acaaagctat cgacgctggt gtagatgctg ttcataaagt agagattcca 1860

gcttcctggt ctaacccagc tgctgatgct cctgctaaag aacttacagg acgtccagag 1920

acagttaaga tggttaagga aatgatggaa cctatcggaa tcatggatgg tgacagcctg 1980

ccagtatcta cattcgtaga caacgctgac ggacagttcg aacttggtgc ttccgcatac 2040

gaaaaacgtg gtactgctgt atctgttcca gaatggaatc cagaagcttg tatccagtgt 2100

aaccagtgtg cgtttgtatg ttcccatgct acaattcgtc ctttcttagt aagcgaagac 2160

gaaattaagg cagctcctga caacatgaaa gttgcagata caaagccaaa ggcaagcgaa 2220

tacaaattta caatgagcgt atctccatta gactgtatgg gatgtggtga gtgtatcaca 2280

gtttgtcctg ctgcagctaa gggcgcatta aagatggttc cacaggaaag ccaggcagca 2340

gaacagccag tattcgatta cttagttgca actgttggaa gaaaaccagg tgctcctgca 2400

gatactacag ttaagggatc tcagttcaac cagccattac ttgagttctc cggatcttgt 2460

gcaggatgtg cagagacatc ttacgctcgt ctgattactc agttattcgg agagcatatg 2520

tacatctcca acgcaacagg atgttcatcc atctggggtg gtcctgcagc aacagctcca 2580

tatacagtaa acaaagattc caaacaggga ccagcttggg caaactcctt attcgaggat 2640

aacgcagagc acggctttgg tatgtatctt ggacagaaga ctttacgtga tcaggctatc 2700

gctaaattag agaagatggc agcatccgat aaagcatccg atgaatttaa agcagcattt 2760

gctaagttca tggaaacaaa agacaataca aaagaaaata cacctgatgc tcaggcatta 2820

atcgctgagg tagaaaaagc agcagcagct ggatgcccag atgcagcaga agtattagag 2880

aagaaacagt atctcgctaa gaagtccgta tggatcttcg gtggtgacgg atgggcatat 2940

gatatcggtt tcggtggatt agaccatgta ttagcttccg gtgagaacgt aaacgttatg 3000

gtattcgata ctgagatgta ctccaacaca ggtggacagg cttctaaagc ttctaacatc 3060

ggtgaggttt gtcagttcgc agcagctggt aaagaaatca gcaagaagag ccttgcagag 3120

atcgctatga gctacggata cgtatacgtt gcacagattg ctcttggtgc taaccctaac 3180

caggcagtta aatgtcttgc agaagcagaa gcttacaacg gaccatccct gatcatcgga 3240

tacgctccat gtgaacttca cggtatcgct aagggtggta tgaaccactg ccaggatgag 3300

atgaagaaag ccgttaaggc tggttactgg aacctcttct ccttcgaccc agctaagaaa 3360

gctgaaggaa agaatccatt cacattaaca tctaaagaag gcgatggatc atatcaggaa 3420

ttcttaaata acgaatcccg ttacactcgt ctgattaagc cattcccaga aagagctgaa 3480

agactcttca acgaatctga gaaggttgct aaggctcgtt acgagcacct gcagagactc 3540

gttgagcttt ataaataa 3558

<210> 45

<211> 387

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 45

atggaggtta aaaagcacat ggctcgtatc gcaggtgtag atttaccaag agaaaaacgt 60

gtagagattg gcttgactta tatttatggt attggcgttg caagttccaa tcgtatcctt 120

gcagaagcaa aagttgatcc taatatccgc tgcaaagact taacagatga tgatgttaag 180

agaatcagtg cagtaatcga tgcgtctcag actgtagaag gtgacttaag aagagaagtt 240

gctatgaaca tcaagagact tcaggagatt ggatgctatc gtggtatccg tcacagaaaa 300

ggacttccgg ttcgtggtca gaaaacaaag accaatgcaa gaacaagaaa aggtccgaag 360

agaacagtag caaacaagaa aaaataa 387

<210> 46

<211> 135

<212> DNA

<213> Ruminococcus torques

<220>

<223> isolation from intestinal tract, Ruminococcus torques

<400> 46

tatataaggg gttcgaggaa ctgtgaaatt attcacagaa aatgtgaaaa actctttaaa 60

atgaaaaaag gccagaaatt gagggaaaga ttgttgataa agcttgactt tatgcggttt 120

tccctctata attaa 135

<210> 47

<211> 837

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 47

atggcaaaat tggttgatgg gaaaagaatt tctcaggaga taaaagacga attaaaagca 60

gaagtggcag atctggcaca gcagggaaag acagcttccc tggcagttat tcaggttggc 120

ggagatccgg catccagcgt atatgtacgc aataagaaaa atgcatgtgc atatatcgga 180

attggatcag aatcttatga attaccggag gagaccaccg aagaagaact gcttgctttg 240

attcaggaat tgaatcagaa ggaagaagta cacggaattc tggtacagct tccgcttccg 300

gctcatattg atgaggagaa ggtaattctt gccatcgatc catccaaaga tgtagatgga 360

tttcatccgc agagcgcagg agcactgatg atcggaagta aaggattttt accatgtaca 420

cctgcgggcg tcattcagct gctgaaacgt tccggtgtgg aaatggaagg aaaaaactgt 480

gtgatcgtag gcagaagtaa catcgttggt aaaccaatgg caatgctgat gcttcgtgaa 540

aatgcaacag ttaccgttgc acattccaga acaaagaatc tgaaagaatt gtgtaaacag 600

gcagatatcc tgatcgttgc catcggaaaa ccgaaattta tcacagaaga atatgtaaaa 660

gagggtgcgg ttgtaattga tgttggaatc caccgtctgg aaggtaagaa attatgcgga 720

gacgtggatt ttgatgttgt ggagccgctt gcaagtgcga ttacaccggt accaggtggt 780

gttggaccga tgacgatcgc aatgctgatg aataattgtg tagagacgat gcgctaa 837

<210> 48

<211> 879

<212> DNA

<213> Dialister invisus

<220>

<223> Diarister invisus isolated from intestinal tract

<400> 48

atgggagaaa ccaaaactgc catatattac aatcacaatt tttactatga tttttcacaa 60

attatcagtg ctgatgatcg ggcgatccag tttggagacg gtgcttatga atggatccgt 120

atacaccatg gacattcttt cgggctttct tatcataccg accgattata tcggtccatg 180

cgccttttag gaatccgccc cgtaacagct cctgatgaat ttactgaaat cgtagaagtc 240

gttgtagaag agtctgaaat tgaagaaggg tatgtgaaga ttattgtgac tcgggggagt 300

gatgaccata acttcacgat ccctgcaaga agtgcgcttc aaccgaatgt ccttgtatat 360

gggaagccta ttgatttgaa tgcaattgaa aaagtccagt ccggagtgaa ctgtattaca 420

cttgatgatg atcgcggaca tcactgcgat attttgagtc tgaatcaatt aaataatatg 480

atggcaagag cagaggcgca gaaaaaaaat tgttatgatg ctgtctttat taaagacgga 540

cttttgacag aagcatctca ttcaaatgta tgtattgtca aagcgggagt tatttggacc 600

ccggcaacga atgagcatat gattcgtggg gttacccgtt ctcttgtact cactcgcgca 660

gctccatctg caggggtaac atctattgat gaggcatctg agcctgtgcg gtttgaagat 720

atgataacag ccgaagaggt atttctgaca aattcccagg atgggatcat tccggtctta 780

tccattgatg gtaaccttat tggcggcggt aaggtgggcg atgtaaccca aaagatccaa 840

gaacattatg cgcatttaat ggaggacgga ctcccgtaa 879

<210> 49

<211> 2616

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 49

atgagcgagc aaacgaaaat caagcacagc cccgccgtgg aaggcaaaaa gcatcacaag 60

gtacgttgga ttgtgctggc ggtgctgctg gtgattgtgg cggcagtggc tgcgtggtat 120

ctgattcgct tcgagtttaa ccgggactat caggcgtaca ttgaagcgcc cgcgggcgcg 180

gcagcgcagt cagaactggc ggcactcacg gacgaaaacc cgaatgtcgc cgcgcctttc 240

cagctgtttg cggaaaacga caacttgaag ctctacgcgg atttggagac ggcgaacgtc 300

gccatctacg acaagcgcag cggcgaaacg atctattcca accccgtgga cgcggatgcg 360

gacaaggtgg cgaacaagac caacaagaac tacttgaaga gccagttcct gctggactac 420

tacaacgccg gtttgacgag cagcacctac gacagctact cgatgagcgt ggcgctgggc 480

aacttccgcg cggcggcgat tgaggacggc attgccttca tctacgacgt gggcgtggac 540

tccatccagt acctgacgcc gtcttacctg ccgacggagc gctacgaggg actctacgag 600

cagctgagcg cacaggcgaa gaaggcagcg gacattgtgt acgaaaagcg cgacgacggc 660

tactggatca gcgcaaccgg tgcgagccgt acccgcgaaa tggcgcgcgt ctccaaggag 720

tttgtggcgc tgggcatgac aaaggcggaa taccttgaaa tggaagcgct ggcgggcaag 780

gaacaggcgg aaacgctggg cttcaccgtg acgctggagt ggcatctggg cgaggacaac 840

gtggaagcca agctgcctgt gagcgcgatt gaagagcgtg gcggcggcaa ggtctaccgc 900

atccagctgc tgccctatat ggcggcagcg ggcagcaacg aaaacggcta catggttgtg 960

ccgaacggtt cgggcagcct gattcgtttc aacaacggca aaaacagtgc tgcggtgtac 1020

agccagtacg tctacgatat ggacttgatt gacggcgact acacgcagac acagacgatt 1080

cagtccgtgc gtctgccatt gtggggcgtg tgccgcgaga acaccagcgt gctggcgacg 1140

attgaaaacg gcgcttcgct ggcggctatc agcgcggatg ttgcggggcg caacaactcc 1200

tacaacaacg cgttcgccat gttcaccctg cgcgggtcgg agctgctgtc tctcttcggc 1260

gcgggcgaaa cggcggaaat gcctatcgtc gaggacaact actacggcga agatctcgtc 1320

atccgctaca cattcctgac ggcggagaac aaggggtaca gcggtctggc gaactactac 1380

cgggcgcgcc tgattgctga aaacgtgctt tctccgctgg aagagacggg cgacattccc 1440

ttctactacg atgtgattgg cggcgtgaag gaaacgaccc acttcatggg cattcagtac 1500

ctgcacgtcc gtgccatgac caccttcgat gaagcggcgg aaattgcgca gtcgctgaat 1560

aaggcgggca tcaagcatca agtgatgaac ttccaaggct ggatgaacgg cggctactac 1620

cacgatgtcg cgaacaacgt gtccgtgctg aggcagctgg gcggcaaaga tcggctggag 1680

aacctgaaca gcgcaatagc acggctgggc ggcgaactgt acgccgacgt cgcgttccag 1740

aaggtgacgc aaatctccaa gcactacatg gagaatcagg agacgtcccg ttattacggc 1800

gcgggttaca ttgcgcagtt cggcgtgacg aatccggcat ccctgcggcg cacgtccgcg 1860

ctgggttaca gcgagaacat ttacaacctg ctgtccccga agttcctgcc gtactacgtc 1920

agtgggttct taagcagcac gaaggattat tctctgaacg gttactccct ccgcgacctc 1980

ggctgtgagc tgcacgcgga taagcgccgc acggagctga ttaaccgcga acaggcgctg 2040

atgattgtcg aaagccagct gcaggcgatt gcggacagcg gcaagaatgt catggtgtcc 2100

ggcggcaacc tctacgccct gaacggtgtc aagcacgtca ttgacgcgcc catgaccgcg 2160

acggagtacg tcatcgtgga tgaaacgatt ccgctttacg agatgattct gcatggctgt 2220

gtggattaca ccggtcaggc gctgaacact atcgtgagcg acgattggca ggcgaagctg 2280

ctgaaaatgg tggaatacgg cgcgtccccg cgctacacct ttacggcgca gcaagcatcc 2340

gacatgaagt acacggcgct gaacagactc tacgccacga ctgtggccaa ctggacggat 2400

accgcggcgg aacagtacgc gtacctgaac gcggcgctcg cctccgtcag cggcgcgcag 2460

atggtgaaac acacgattct ctctgatacg ctgcgcagcg tcgaatacga caacggcgtg 2520

accatctaca tcaactacgg cagtcaggat gcgcaggcgg gcggcggcac cgttcccgcg 2580

aaggcgtatc tggtcacagg aggtgcaaac gaatga 2616

<210> 50

<211> 3696

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 50

atgattagtt tcttcaaaaa tgtgtcgggt tcgattatcg cagttgatac cgaccacaag 60

ctggaaaaca gcgaaattga aaaattaaaa tggttgttca acgacgccca gctgcttgac 120

gcctctacgg tggaaggcaa gtttgtggga ccgcgccggg agatgataac cccttggagt 180

acgaacgcgg tggaaataac ccagaacatg ggtctttccg gagttaaacg cattgaagag 240

tttttcccga caacagcaaa aaatccaacc tacgacaaga tgcttcagcg cctttatgag 300

aatcttggcg ccgacatctt tgaaattctc aaaaaaccgg atgagattgt ttacatctcc 360

gacatcgagg catacaacga gcaagaaggc ttggcgctca atccggaaga ggtggattac 420

ctgaagtcgt tgagcgcaaa gctcaaccgt ccgctgaccg acagcgaggt gttcggtttc 480

tctcaggtca actctgagca ctgccgtcat aaaatcttca acggcgtgtt tgtaatcgac 540

ggtgtggaga aaccgtcttc tctttttaag ctcattaaaa agacatcgca ggaaaatcct 600

aataagcttg tgtcagcata taaagacaac gtggcttttg tagaaggtcc ggtgatagag 660

cagtttgctc cgaaaaatcc gcaaggcccc gacttcttcc aaatcaagga tgttgaaaca 720

gtaatctcgt tgaaggcgga aacccacaac ttcccgacga ccgtagagcc gttcaacggc 780

gcggctacag gaacgggagg tgaaatccgt gaccgcctcg gaggaggaaa ggcgagtctg 840

ccgatagccg gaacggcggt ctatatgacc tcgtatccgc gcacggagga aaaccgtgtg 900

tgggaggctt cggcgatggc gccgcgcaaa tggctttacc aaacgcctga ggaaatcctt 960

atcaaggcat caaacggtgc gtccgacttc ggaaacaaat tcggacagcc gctgatttgc 1020

ggctctgtgc tcacgttcga gcataaggaa aacggagagg aatttgccta cgacaaagtg 1080

attatgcttg ccggtggcgt gggattcgca gcaaagcgcg atgccctgaa gggcgagcct 1140

aagcctcacg aaagcgtggt gctgatgggc ggcgacaact atcgtatcgg tatgggcggc 1200

ggcgccgtgt cgtcggtcaa caccggacaa tacgacaact ctatcgagct caacgccgtg 1260

cagcgcgcca atccggagat gcagaagcgt gtgtcaaacg taatccgtgc gcttgccgag 1320

tctgacaaca atccgattgt gtcaatccac gaccacggtg ccggcggaca cttgaacgct 1380

ttgtcggaac tcgttgagtc gacaggcgga aaaattgaaa tcggagctct cccggtaggc 1440

gaccctacac tgtcgtcgaa agaaatcatc ggcaatgagt cgcaggagcg tatgggcatg 1500

gtgattgagc aaaaagacat tgagtgggtg cgcacaattg ccgagcgcga acgcgctccg 1560

ttctatgtgg ttggagaaac caccggcgac catcgttttg tctttgagca gaccgacggt 1620

gtgtgcccaa tcgacttggc tatgagcgac atgttcggca gctcgccaaa gacttatatg 1680

accgactcta cggtagaacg ccgtctgaag tcggttaaat acaagaaatc agacgtcaac 1740

acatatatag aaaatgtgct tcagctcgag gcggttgcct gcaaggactg gttgacaaac 1800

aaggttgacc gttcggtgac aggcaaggtg gcacgccagc aatgtcaggg cgagatacag 1860

ttgcctctca gcgactgcgg cgttgtggca cttgactata caggaacgaa aggcatcgca 1920

acatcaatcg gccacgctcc gcaattggct ttggctgatt cggcacaagg ctctgtggcg 1980

tcgatagcgg aagctttgac aaacattgtg ggcgcgcctc tcagcgacgg attgaagggc 2040

gtttcgctca gcgccaactg gatgtggcct tgccgcaacg ccggcgagga tgccgctctc 2100

tacaaggcag tggaggcatg cagcgacttt gcctgccgtc ttggcatcaa catccctact 2160

ggcaaggaca gcctctcgat gacgcagaaa tacggcaaga aaaaggtgat ggctccaggc 2220

acggtgatta tctcggctgc cgctgaggtg tctgacgtgc gcaaggttgt gtcgccggtg 2280

gttcaaaaca agaagaacac cactcttgtc tacgttgatt tcagcaattc tccgcttgcg 2340

ctcggtggct ctgcgttcgc gcagatgctc ggcagtgtgg gcagcgacgt gccacgcatc 2400

gactccgctg aatatttcgc aaaggcattt gccgctgttc agtcgcttgt cgacgaaggc 2460

aaggtgcttg caatgcacga tgtctcggcg ggcggtcttg tcaccacgtt gcttgagatg 2520

acattcgcca acacgaaggg cggtttgaac atcaacctcg acggatttga cgacaaggat 2580

gtcgtgaagc tgttgtttgc tgaaaacagt gctgtcgtga tgcaagttga gaacaaaaac 2640

ttgaaggctg tggaagcggc tgtcgacggt ctgcatgccg tggcaatcgg cacggttcag 2700

gacgagcgtg tggttctcgt gtcaaatctc ggttgcgagt atctgctcga cattgattat 2760

ctgcgcgact tgtggttcaa gtcatcgtat ctgctcgacc gccgtcagag cggcgcggca 2820

tgtgcgaaaa agcgttttga gaactataag aagcagccta tacgctataa attgccgaag 2880

gatttcaaag gcacgcttgc ctcttacgga cttaccggca accgccgcac tccgacaggc 2940

atcaaggctg caattatccg cgagaagggc gtgaacggcg accgcgagat ggcatattcg 3000

ctttatctcg ccggctttga tgtgaaagac gttcacatga ccgaccttat gtcgggtcgc 3060

gaaactcttg acgacgtcaa tatgattgtg ttctgtggag gattctccaa ttccgacgtg 3120

ctgggctcgg cgaaaggatg ggctggcggc tttgtctgga acgagaacgc aaaggctgct 3180

ctgcagcgct tcttcgcccg taaggacact ctgagccttg gcatctgcaa cggttgccag 3240

ctgatgatag agctgaacct catcaatccg gaattcaagc ataaggcaac gatggagcac 3300

aacgactcgc ataaattcga gtcgcagttc cttggcctcc gcattccgga aaacaactcg 3360

gtgatgttca agtcgctttc aggctccaaa cttggcatct gggtggctca cggcgaaggt 3420

aagttcaatt tgcctgaagg cgttgaaaac tacaacgtgg ttgcggaata tgcgtatgcg 3480

tcatatccag ccaaccctaa cggatcgcct aaggctgttg cgggtatagc gtcggccgac 3540

ggccgccatt tggcgatgat gcctcacctt gagcgtgcca tcttcccatg gcagtgcgga 3600

ttctatcctg aaaaccgccg tcttaccgac gaggttacac cttggataga ggcgtttgtc 3660

aatgcacgca agtggattga gcagaacaag aaataa 3696

<210> 51

<211> 1524

<212> DNA

<213> Ruminococcus obeum

<220>

<223> isolation from intestinal tract, Ruminococcus obeum

<400> 51

atgaaattca cagacaaatt taaagcaaag aagacagaaa atcggggaaa tccggcaaat 60

gaagctccaa agaaagaaaa gaagcctctg aagcagaaga tcaaggattc catgaacaaa 120

aagtatctca aaaatggttc ttacagtgta gtcatcagtg ccatttttat tgtaatcgta 180

cttgtagtaa atatgatcgt cggctcactt ccatccaaat atacggaagt tgatgtcagc 240

agcgaaaagc tgtacagcat cagcgatgat acaaaggatt tcctgaaaaa actggacaaa 300

gatatcacaa tctatcaggt ggtccagagt ggttctgaag atgagaccat caaaaaactt 360

ctggaaaaat atgatgagga atccgatcat attaccgtag aacagaaaga cccggtagta 420

aatccgaaat ttacatccga atatacaagt gacgatgttg ctgcaaacag cctgatcgtt 480

gtatgcggtg acagaagcaa agtggtaaat tacagcagta tttatgaatc ttctatagac 540

tacaatactt atcagtctac aacgacagga tttgacggag aaggccagat tgacagtgca 600

atttcctatg tgacatcaga aaatcttcca gtgctttata cgctggacgg acatggtgaa 660

aaagaactgg attccacact tcaggaggat atccagaagg caaatattga cattaagtcc 720

ctcaatctga tcacagaaga aaaagttccg gatgatgcgg catgcctgtt gatcaatgca 780

cctgcttctg atatttccga atctgaaaaa gatgcaatta ttgattatct ggaaaatggc 840

ggaaaagcca tgatcttctc tgattataca gaagaatcta tggaaaactt tgatgctgtt 900

cttgcaaatt atggtgtgga aagaacagat ggtattgtga tcgagggtga ttcacagcat 960

tatgcacaga tgccttatta tctggtaccg acagtcaaca gcacagatgc aatctctgat 1020

tttgcatcca aaggctatta tgtcctgatg ccgtatgcgc agggaatcaa acagacagat 1080

aatcttcgtg atacagttac agtcaacagc cttctgacta cttctgacag tgcatattca 1140

aaagtagatg tacacagtgg ttcgattgac aagaccgacg atgatgttga cggtccgttt 1200

gacctgggtg taagtattac agaaacttta gatgatgaca aagaaactca gattgtttat 1260

tatacaagtt ccaatcttat ggacagccag atcaaccaga tggtatccgg cggaaacgaa 1320

cagatgatca tgagttctct gaactggatg tgcagctctg atgaaacttc tgtcatctct 1380

gttccgtcca aaaacctgca gatttcctat ctgacgctga ctgcttatga tgtaagcttc 1440

tggaaaatta tggtaatggg aattatcccg gttattttcc ttgtcattgg atttatggta 1500

tggctgaaaa ggagaaaagc ataa 1524

<210> 52

<211> 1020

<212> DNA

<213> Bacteroides intestinalis

<220>

<223> isolated from intestinal tract, Bacteroides intestinalis

<400> 52

ataaatcata aatctgaaat cataaatcat aagatggata ccaaatcaca aaacagtaac 60

atgctactag cgttcttaac gctgacaggc gtcattgcca ttgtagccgt agtgggtttc 120

ttcatgttgc gcaaaggacc ggaaatcgta caaggtcaag ctgaagtcac agagtatcgg 180

gtatcgagca aagtaccggg acgaattctg gaattccgcg taaaggaagg acaaagtgta 240

caagccgggg ataccctcgc catactggaa gctccggatg tgttagctaa gctggaacag 300

gctcgggccg cagaagctgc tgctcaggca cagaatgaga aagcactgaa aggcgcccgt 360

catgaacagg tacaagctgc ctttgagatg tggcaaaaag ccaaagcagg acttgaaata 420

gccgagaaat cttacaaacg tgtgaagaat cttgctgatc agggcgtgat gtcagcccaa 480

aagctggatg aggttactgc acagcgggac gctgccgtcg ctacagaaaa agccgccaaa 540

gctcaatacg acatggcaaa aaacggtgcg gaacgagaag ataaagcagc agctgccgcc 600

ttggtagacc gtgccaaagg agccgttgca gaagtagaat cttatatcaa agaaacttat 660

cttatagccc agacagccgg agaagtttcg gaaatcttcc ccaaagtagg tgaattggta 720

ggaaccggtg caccgatcat gaacatagcc atactggatg atatgtgggt aacttttaat 780

gttcgggaag atttgctgca aggtctgaca atgggaactg aattcgaagc tttcgttccg 840

gcattggata aaaatatccg cctgaaagtg aactacatga aggatcttgg tacttatgca 900

gcctggaagg ctacgaaaac aaccgggcaa tttgatctga agacttttga ggtaaaagct 960

ttaccacagg agaaagtgga aggcttacgt cccggaatgt cggtgatact gaagaagtga 1020

<210> 53

<211> 1053

<212> DNA

<213> Klebsiella variicola

<220>

<223> isolation from intestinal tract, Klebsiella variicola

<400> 53

atggttttca gaatcgcaag ctccccctat acccataacc agcggcagac ctcgcgtatt 60

atgctgctgg tgctgctcgc cgccgtgcct ggcattgtgg tccagacctg gtttttcggc 120

tggggtaccg tactgcagat tgtcctcgcc gcattgacgg cctgggcaac ggaagccgct 180

attctcaaac tgcgcaaaca gcatattgtg gcgaccctga aggacaactc cgccctgctc 240

actggcctgt tgctggcggt gagtattcca ccgctggccc cctggtggat ggtggtgctc 300

ggcactgcct ttgccgtggt aatcgccaag cagctgtacg gtggattagg ccataacccc 360

ttcaacccgg cgatgatcgg ctacgtcgtg ctgttgattt cattcccggt acagatgacc 420

tcctggctgc cttcatatga aattgccgcc caggttccgg cgttcagcga tgtgctgcag 480

atgatcttca ccgggcatac cgccgccggg ggcgacatgg ccagcctgcg actgggtatc 540

gacggcatca gccaggccac cccgctggat acctttaaaa cctctctgca tgccgggcat 600

agcgtccagc aggtgctgca attgccggtt tacggtggcg tactggcggg cctcggctgg 660

cagtgggtga atatcgcctg gctggcgggc ggtctgttcc tgctgtggca gaaagcgatc 720

cgctggcata ttccggtcag ctttctcgtt agccttggtc tgtgtgccac cctcggctgg 780

ttattctcgc cgcaaagcct ggcgtcgccg cagatgcatt tgttctctgg cgccaccatg 840

ctcggtgctt tctttattct gaccgacccg gtgacggctt ctacaaccaa ccgcggccgc 900

ctgatcttcg gcgccctcgc cggcctgctg gtgtggctta ttcgcagctt cggcggttac 960

ccggatggcg tggcatttgc cgttctgctg gcgaatatta ccgtgccgct gatcgattat 1020

tacacgcgcc cgcgcgtgta cggccaccgt tga 1053

<210> 54

<211> 1830

<212> DNA

<213> unknown

<220>

<223> isolated from intestinal tract, not identified

<400> 54

cttttcaaca gtgcgaccga tccctcgggc gacggcagcc acatccgcaa ttttacgtcc 60

aagggcgttt atctgctgct ctacagccgc ggcgagccgt cgtatgagac gggtgacgcg 120

ggcgcgcttc gctatgcgcc gcagcgcatc gcggagaagc tcggcgagac catcgagacc 180

gtctacggcg agcgggaggg cggctttctg cgcgccctgc tcctcggcga caagcgctat 240

cttgacgtgg aggattcgtc gaacctgagc gaggcgggca tctatcacat caccgccgtc 300

tcgggcctgc actgtatgtt cctcgtcgag ctgcttgacc gactgctccg cgcgtcgcac 360

cggcgtacac gctgcgcggt gacgatcccg ctgctgtttc tctacgcggt gatggtcggc 420

gcgtcgccga gcatcctgcg cgcctgcgtg atgctctcga tgacgcagct cgcgccgctt 480

ttcaggcggg agaaggaccc gccgacggcg ctcggccttg cattgctgct cattttgctc 540

aaaaatccct acgccattgc gagcatcagc ttacagctct ccttctcggc ggtggcgggc 600

cttgtgtggc tgacgccgaa gatgacggga gccatccaca ccggaagcag gctcctgcgc 660

ttcgtgctca catcgttcgc gacgacgctc ggggcgctgg cgttctccac gccgcttggg 720

gcatattatt tcggctcctt ctccgtcgtt tcacttttga gtaacctcct gtgcctgtgg 780

atggtgagcc ttgtcttcgg cacgggtctg ctcggattgc tgctgagtat ggcggctccg 840

gcgctcggcg cggcctttgt gccgctcgtg tcgctcagcg tgcggtatgt gctttttgtt 900

tcgggactgc tggaggatct gccgctccac gcgctctatt tcaacaccgc gctttccgtg 960

ctgtggctgt tctacgtcta tgcgctcttc gccgtctgcg ccatcgcgcg gcgggggcgg 1020

taccgctggt ggatcgccgg cgcgctggcg gtgacgatgc tccttgcgac agcgcgcttc 1080

aacgcgctgc aatttgagcg cgggagcctg catgtcacgg cgctcgatgt cggacagggc 1140

gagagcgttg cgctgctctc caggcgtcat ggcgtgctcg tggactgcgg cagcagcaat 1200

tcctacatca gcgcgggcga cgtggccgcg gatcatttgc tcagcgccgg tatccggcgg 1260

ctcgacgcgg tggtgctcac gcactatcac gccgaccatg ccaacggtct ggcgctgctg 1320

cttgcccgtg tcggcgtgga tacactgtat ctgcccgaca ttgcggagga ggacggcgaa 1380

aagtccgagg tgctcgcgct ctcggagcgc tacggcgtgg aggtccgcta tgtgacggag 1440

gaaacgcaga ccgccgtcgg cgaggcgtcg ctgacgctct atccgccggt gggcgagggc 1500

ggagaaaacg agctggggct gacgatcctc tgctcggcgg gcgactttga tacgctcatc 1560

accggcgata tggacagcag gaccgagcgc gtgctcgtga gcagctatcc gctgccggac 1620

attgaggtgc tgctcgtcgg acaccacggc tcgcgctact cgacgagcga ggagctgctg 1680

gagaccgtca cgccggaggt cggcgtggtg agcgtcggca gcaacagcta cgggcatccg 1740

acgcgggacg cgctgctgcg cctgacggac gcgggcgtga ccgtttaccg gacggacctg 1800

cagggaaata tttatatcac agtgaattga 1830

Claims

1. A biomarker panel for predicting a subject's microbiota-associated disease, consisting of:

SEQ ID NO: a sequence of 1 to 54, and,

wherein the disease is obesity.

2. A kit for determining the biomarker set of claim 1, comprising primers required for PCR amplification designed according to the biomarker set of claim 1.

3. A kit for determining the biomarker set of claim 1, comprising one or more sequences according to SEQ ID NO: 1 to 54, respectively.

4. Use of reagents for detecting the biomarker set of claim 1 in the manufacture of a kit for predicting the risk of obesity in a subject comprising primers and/or probes required for PCR amplification, the primers and/or probes designed according to the biomarker set, the predicting comprising:

(1) collecting sample j from the subject;

(3) calculated from I as follows_jIndex of expression:

A_ijis the relative abundance of marker i in sample j, wherein i refers to each of the biomarkers in the biomarker set;

n is a first subset of all patient-enriched markers among the selected biomarkers associated with the abnormal condition,

m is a second subset of all control-enriched markers among the selected biomarkers associated with the abnormal condition,

wherein the biomarkers associated with abnormal conditions comprise: a subset of the markers in the set of gene markers that are enriched in all obesity,

| N | and | M | are the number of the biomarkers in the first subset and the second subset, respectively,

wherein an index greater than a threshold value indicates that the subject has or is at risk of developing an abnormal condition, which is obesity,

where | N | is 24, | M | is 30, and the threshold is 0.5834.