CN106726868B - A kind of preparation method for the China rose anthocyanidin having whitening capability - Google Patents
A kind of preparation method for the China rose anthocyanidin having whitening capability Download PDFInfo
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- 240000008254 Rosa chinensis Species 0.000 title claims abstract description 71
- 235000000664 Rosa chinensis Nutrition 0.000 title claims abstract description 70
- 229930014669 anthocyanidin Natural products 0.000 title claims abstract description 43
- 150000001452 anthocyanidin derivatives Chemical class 0.000 title claims abstract description 43
- 235000008758 anthocyanidins Nutrition 0.000 title claims abstract description 43
- 235000000100 Hibiscus rosa sinensis Nutrition 0.000 title claims abstract description 32
- 235000016785 Rosa della China Nutrition 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 230000002087 whitening effect Effects 0.000 title claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000001256 steam distillation Methods 0.000 claims abstract description 11
- 239000002826 coolant Substances 0.000 claims abstract description 10
- 238000001816 cooling Methods 0.000 claims abstract description 6
- 238000004821 distillation Methods 0.000 claims abstract description 6
- 239000004615 ingredient Substances 0.000 claims abstract description 5
- 210000002752 melanocyte Anatomy 0.000 claims abstract description 5
- 238000003860 storage Methods 0.000 claims abstract description 5
- 239000000706 filtrate Substances 0.000 claims abstract description 3
- 230000002401 inhibitory effect Effects 0.000 claims description 8
- 239000003205 fragrance Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 2
- 239000008132 rose water Substances 0.000 claims description 2
- 230000014759 maintenance of location Effects 0.000 claims 1
- 239000000047 product Substances 0.000 abstract 1
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 20
- 230000031700 light absorption Effects 0.000 description 12
- 235000004515 gallic acid Nutrition 0.000 description 11
- 229940074391 gallic acid Drugs 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 9
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 8
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 7
- 229930003935 flavonoid Natural products 0.000 description 7
- 150000002215 flavonoids Chemical class 0.000 description 7
- 235000017173 flavonoids Nutrition 0.000 description 7
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 6
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- -1 DPPH free radical Chemical class 0.000 description 5
- 210000003491 skin Anatomy 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 4
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 4
- 229930003268 Vitamin C Natural products 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 4
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 4
- 229960001285 quercetin Drugs 0.000 description 4
- 235000005875 quercetin Nutrition 0.000 description 4
- 235000019154 vitamin C Nutrition 0.000 description 4
- 239000011718 vitamin C Substances 0.000 description 4
- 238000005259 measurement Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- 206010015150 Erythema Diseases 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 206010030113 Oedema Diseases 0.000 description 2
- 206010033733 Papule Diseases 0.000 description 2
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 2
- 206010040880 Skin irritation Diseases 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 2
- 235000011056 potassium acetate Nutrition 0.000 description 2
- 230000036556 skin irritation Effects 0.000 description 2
- 231100000475 skin irritation Toxicity 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- OCZVHBZNPVABKX-UHFFFAOYSA-N 1,1-diphenyl-2-(2,4,6-trinitrophenyl)hydrazine;ethanol Chemical compound CCO.[O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1NN(C=1C=CC=CC=1)C1=CC=CC=C1 OCZVHBZNPVABKX-UHFFFAOYSA-N 0.000 description 1
- 206010012442 Dermatitis contact Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 101000891579 Homo sapiens Microtubule-associated protein tau Proteins 0.000 description 1
- 231100000002 MTT assay Toxicity 0.000 description 1
- 238000000134 MTT assay Methods 0.000 description 1
- 102400000740 Melanocyte-stimulating hormone alpha Human genes 0.000 description 1
- 101710200814 Melanotropin alpha Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 230000001857 anti-mycotic effect Effects 0.000 description 1
- 239000002543 antimycotic Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000007541 cellular toxicity Effects 0.000 description 1
- 208000010247 contact dermatitis Diseases 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 102000057063 human MAPT Human genes 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000008099 melanin synthesis Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L sodium carbonate Substances [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- WHNFPRLDDSXQCL-UAZQEYIDSA-N α-msh Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(N)=O)NC(=O)[C@H](CO)NC(C)=O)C1=CC=C(O)C=C1 WHNFPRLDDSXQCL-UAZQEYIDSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of preparation methods of China rose anthocyanidin for having whitening capability, new fresh China rose is distilled with steam distillation, the ratio of water and flower is 4.5-5.5:1, water vapour after distillation is through cooling, obtain coolant liquid, when the coolant liquid weight distilled out is that 3-4 times of Chinese rose petal weight is added, that is, stop distillation；After steam distillation, China rose is boiled 0.5-1.5 hours jointly and is filtered in distiller bottom China rose Liquid Residue and distiller, filtrate removes highly polar ingredient by DIAION HP-20 tubing string, it is concentrated in vacuo and drains after collection efflux, it is freeze-dried up to Chinese rose anthocyanidin again, set -20 DEG C of ice storage hiding.Product of the present invention can inhibit melanocyte and generate melanin.
Description
Technical field
The present invention relates to cosmetic material fields, more particularly to a kind of China rose anthocyanidin preparation side for having whitening capability
Method.
Background technique
China rose (scientific name: Rosa chinensis Jacq.): referred to as spending middle queen, also known as " Chinese rose ", be it is evergreen,
Half evergreen short shrub, four Ji Kaihua ﹐ are generally red color ﹐ or pink colour, occasionally have white and Huang color ﹐ to can be used as ornamental plant, can also
As medicinal plant, also known as Chinese rose.There are three natural mutation, modern distance education system flower pattern multiplicity, have single-lobe and polyphyll, there are also Gao Xinjuan
The grace flower pattern such as side；Its is beautiful in colour, abundant, not only there is that red, powder is yellow, the monochromes such as white, and there are also the kinds such as colour mixture, silver-colored side；It is most
Kind has fragrance.Chinese rose it is various in style, have nearly ten thousand kinds in the world, China also has thousand kinds or more.
Natural florescence August is to April next year, and flower is at large size, from inside to outside, is in divergence form, has strong fragrance, can be widely used for
Horticultural gardening and cut-flower.Chinese rose it is adaptable, resist cold, it is cold-resistant, though ground plant, potting, suitable for beautifying the yard, decorateeing
Gardens arrange flower bed, plant colored hedge, flower stand, and Chinese rose cultivation is easy, and can make cut-flower, for doing bouquet and the various gailys decorated basket, China rose
Piece extractable essence, and can be used as medicine.Also there is preferable antimycotic and collaboration overriding resistance fungi activity.
Summary of the invention
The purpose of the present invention is to provide a kind of China rose anthocyanidin preparation methods for having whitening capability.
Technical solution provided by the invention is as follows:
An object of the present invention is to provide a kind of China rose anthocyanidin preparation method for having whitening capability, including such as
Lower step: new fresh China rose is distilled with steam distillation, water and the ratio spent are 4.5-5.5:1, the water vapour after distilling
Through cooling, coolant liquid is obtained, when the coolant liquid weight distilled out is that 3-4 times of Chinese rose petal weight is added, that is, stops distillation；With
After steam distillation, China rose is boiled 0.5-1.5 hours jointly and is filtered in distiller bottom China rose Liquid Residue and distiller, filter
Liquid removes highly polar ingredient by DIAION HP-20 tubing string, collects be concentrated in vacuo after efflux and drain, then freeze-dried is
Chinese rose anthocyanidin is obtained, -20 DEG C of ice storage hiding are set.
Preferably, distilling new fresh China rose with steam distillation, water and the ratio spent are 5:1, with steam distillation
Coolant liquid through cooling gained stops steaming when the coolant liquid weight distilled out is that 3-4 times of Chinese rose petal weight is added
It evaporates；After steam distillation, China rose is boiled 1 hour jointly and is filtered in distiller bottom China rose Liquid Residue and distiller, filter
Liquid removes highly polar ingredient by the DIAION HP-20 tubing string to configure completion, is concentrated in vacuo and drains after collection efflux, then
Up to Chinese rose anthocyanidin, -20 DEG C of ice storage hiding are set within freeze-dried 24 hours.
It is still a further object of the present invention is to provide a kind of purposes of China rose anthocyanidin, it is used to inhibit DPPH free radical.
It is still a further object of the present invention is to provide a kind of purposes of China rose anthocyanidin, it is used to inhibit to inhibit tyraminase
Activity.
It is still a further object of the present invention is to provide a kind of purposes of China rose anthocyanidin, for inhibiting melanocyte raw
At melanin.
Seen from the above description, the present invention provides a kind of China rose anthocyanidin preparation method for having whitening capability, this hairs
The China rose anthocyanidin of bright preparation can be used for inhibiting DPPH free radical, inhibit tyrasamine enzymatic activity or inhibit melanocyte
Generate melanin.And to human skin without stimulate the reactions such as redness, edema, papule, bubbles.
Detailed description of the invention
Fig. 1 is that the season anthocyanidin of the embodiment of the present invention 5 inhibits the experimental result picture of DPPH free radical；Wherein abscissa is dense
It spends (ppm), ordinate is inhibiting rate (%)；
Fig. 2 is 8 patch tests result of the embodiment of the present invention；
Specific embodiment
Embodiment 1
Chinese rose anthosin dew, Chinese rose anthocyanidin production technology:
1. distilling new fresh China rose (selecting bright red system's China rose) with steam distillation, water and the ratio spent are 5:1, with
Water vapour after distillation obtains coolant liquid through cooling, when the coolant liquid weight distilled out is that 3 times of Chinese rose petal weight are added,
Stop distillation, this is Chinese rose essence dew, containing natural Chinese rose fragrance, can be used as natural Chinese rose water and is used.
2. it is small that China rose Liquid Residue and the China rose in distiller of distiller bottom boil 1 jointly after steam distillation
When filter, filtrate is by removing highly polar ingredient to configure the DIAION HP-20 tubing string completed, and vacuum is dense after collecting efflux
Contracting is drained, then freeze-dried 24 hours up to Chinese rose anthocyanidin, set -20 DEG C of ice storage hiding.
Total Flavonoid Content of 2 Chinese rose anthocyanidin of embodiment measures
Flavonoids can form stable yellow misfit object under alkaline condition with aluminium chloride, and in wavelength 415nm
Under have extinction, when color is deeper, Flavonoid Content is higher.Take one 96 porose discs that sample or various concentration standard items 20mL is added again
60mL ethyl alcohol (95%), 10% aluminium chloride of 4mL (aluminum chloride), 4mL 1M potassium acetate is sequentially added
(potassium acetate) and 112mL deionized water are protected from light 30 minutes, at room temperature by ELISA reader in wave
Long 415nm surveys its light absorption value.According to Flavonoid Content number in light absorption value discriminating test sample, previously system is substituted into using its light absorption value
Quercetin (quercetin) standard curve of work makes the concentration expression relative to Quercetin.Finally, conversing every gram of sample
In contain how many milligram Flavonoid Content.
Total Flavonoid Content measurement result of Chinese rose anthocyanidin
| Sample | Total Flavonoid Content (mg Quercetin eq./g) |
| Chinese rose anthocyanidin extract | 26.6±2.0 |
The total phenol content of 3 Chinese rose anthocyanidin of embodiment measures
The measurement of total phenol content for standard items, makes the gallic acid of various concentration with gallic acid (gallic acid)
The calibration curve of light absorption value surveys the light absorption value of 750nm using ELISA, and using light absorption value as the longitudinal axis, gallic acid concentration of standard solution is
Horizontal axis draws standard curve, and calculates quantitative linearity regression formula.Light absorption value measured by sample is added by gallic acid calibration curve
After conversion, the Determination of Polyphenols that sample to be tested can be obtained is equivalent to the content of how many gallic acids, the amount of gallic acid
Higher, the antioxidant activity of representation compound is higher.
Gallic acid standard curve making:
(1) the gallic acid aqueous solution that compound concentration is 12.5,25,50,100,200 and 400 μ g/mL respectively.
(2) standard aqueous solution of the 50 above-mentioned concentration of μ L is respectively taken, 50 μ L forint phenol reagent (Folin-Ciocalteu's are added
Phenol reagent), after evenly mixing in being stored at room temperature 20 minutes.
(3) adding 100 μ L concentration is 20%Na2CO3Aqueous solution, mixing be placed on room temperature reaction 10 minutes, measurement in
The light absorption value (working as blank control with water) of 750nm.
It (4) is the longitudinal axis using light absorption value, gallic acid concentration of standard solution is that horizontal axis draws standard curve, and calculates quantitative
Linear regression formula.
The total phenol content of Chinese rose anthocyanidin measures
| Sample | Total phenol content (mg Gallic acid eq./g) |
| Chinese rose anthocyanidin extract | 102.2±1.4 |
4 Chinese rose anthocyanidin Cytotoxicity evaluation of embodiment (Colorimetric MTT assay)
SCC25 cell (human tau neck squamous cancer cell line) and HaCaT (human skin horn cell) is selected to assess the moon
Season 72 hour cell toxicity test of anthocyanidin, Chinese rose anthocyanidin 100mg/mL slightly has a little toxicity to SCC25 cell as the result is shown,
But cell survival degree is still above 70% or more；Anthocyanidin, which does not have toxicity, to be shown to HaCaT (human skin horn cell).
The assessment of 5 Chinese rose anthocyanidin of embodiment inhibition DPPH free radical
Method: being protected from light 30 minutes after taking 99 μ L DPPH ethanol solutions (100 μ L) to mix the extract of 1 μ L, finally in
Light absorption value (BioTek, SynergyTM2, USA) is measured under wavelength 517nm.Compared with control group Vitamin C.
For anthocyanidin when for 100ppm concentration, display can reach 94.2% rejection ability as the result is shown, show China rose
Blueness is known as the efficiency of very excellent inhibition DPPH free radical.
| Sample ID | Inhibiting rate (%) |
| Vit C (control group) | 97.7±0.9 |
| Anthocyanidin | 94.2±0.2 |
6 Chinese rose anthocyanidin of embodiment inhibits tyraminase Activity Assessment
Under concentration 100ppm, Chinese rose anthocyanidin shows the inhibition Tyrosinase effect more than 50%.
| Sample ID | Inhibiting rate (%) |
| Vit C (control group) | 98.2±1.0 |
| Anthocyanidin | 58.6±2.2 |
7 Chinese rose anthocyanidin of embodiment inhibits the Activity Assessment of melanocyte (B16) generation melanin
By B16 cell, with 1 × 105The density of/mL is inoculated in cultivated 24 hours in 96 orifice plates after, by extract and control
Group vitamin C is mixed in taking after reaction 72 hours without phenol red (phenol red free) culture solution containing 100nM α-MSH
100 μ L of supernatant measures light absorption value (BioTek, SynergyTM2, USA) in 96 orifice plates, in 405nm wavelength.Calculate extract
With the light absorption value of vitamin C itself, conversion extract and vitamin C act on inhibition Melanin productions ability after cell.
Chinese rose anthocyanidin had inhibitory effect under 100ppm concentration, in height under 72 hours action time as the result is shown
The dosage 500ppm of concentration can have found the effect of significantly inhibiting B16 cell to generate melanin.
Embodiment August season anthocyanidin skin irritation tests (patch test)
Assessment result: Chinese rose anthocyanidin skin irritation test result is shown in observation in 24,48,72 hours and pierces without skin
Swash property reaction.
Experimental method: according to the regulation of International Contact Dermatitis study group (ICDRG), is observed in a manner of vision,
By obtained sample configuration at 10% aqueous solution, then 0.1ml is taken to be placed in the plastics that 1.0 × 1.0cm encloses gauze with pipette
Disk separately takes 0.1ml filtering secondary water as blank control group, is attached to Medial upper arm, is fixed with breathable adhesive tape, avoids having a bath and do
It is wet, every 24 hours gauzes more renewed, and the tested substance of addition 0.1ml and blank group again, it observes 72 hours.Observation skin has
Without stimulate the reactions such as redness, edema, papule, bubbles.(experiment person-time is 10 people).
Claims (3)
1. a kind of China rose anthocyanidin preparation method for having whitening capability, it is characterised in that: distilled with steam distillation fresh
The ratio of China rose, water and flower is 4.5-5.5:1, and the water vapour after distilling obtains coolant liquid through cooling, when the cooling distilled out
Liquid weight is when 3-4 times of Chinese rose petal weight is added, that is, to stop distillation；After steam distillation, distiller bottom China rose
China rose is boiled 0.5-1.5 hours jointly and is filtered in Liquid Residue and distiller, and filtrate removes high pole by DIAIONHP-20 tubing string
Property ingredient, be concentrated in vacuo and drains after collecting efflux, then is freeze-dried up to Chinese rose anthocyanidin, set -20 DEG C of ice storages and hide.
2. a kind of China rose anthocyanidin preparation method for having whitening capability, it is characterised in that: the coolant liquid distilled out is Chinese rose essence
Dew, containing natural Chinese rose fragrance, uses as natural Chinese rose water.
3. the purposes of China rose anthocyanidin is used to prepare the preparation for inhibiting melanocyte to generate melanin, wherein the moon
Season, flower anthocyanidin was prepared using method of claim 1, and the use concentration of China rose anthocyanidin is 100-500ppm.
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| CN201611026841.5A CN106726868B (en) | 2016-11-22 | 2016-11-22 | A kind of preparation method for the China rose anthocyanidin having whitening capability |
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|---|---|---|---|
| CN201611026841.5A CN106726868B (en) | 2016-11-22 | 2016-11-22 | A kind of preparation method for the China rose anthocyanidin having whitening capability |
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|---|---|
| CN106726868A CN106726868A (en) | 2017-05-31 |
| CN106726868B true CN106726868B (en) | 2019-10-29 |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1511583A (en) * | 2002-12-28 | 2004-07-14 | 石家庄制药集团制药技术开发有限公司 | Chinese rose extract and its preparing method and use |
| CN101311225A (en) * | 2007-05-26 | 2008-11-26 | 王晓明 | Process for abstracting kohlrabi pigment |
| CN102048662A (en) * | 2009-10-30 | 2011-05-11 | 爱普香料集团股份有限公司 | Method for manufacturing rose water containing natural rose pigment |
| CN102838884A (en) * | 2012-01-18 | 2012-12-26 | 天津天康源生物技术有限公司 | Prevention method of anthocyanin pigment crystal precipitate |
| CN103044953A (en) * | 2011-10-13 | 2013-04-17 | 黎嘉纯 | Extraction process for Chinese rose pigment |
| CN105106047A (en) * | 2015-08-07 | 2015-12-02 | 刘学勤 | Water-replenishing and skin-tendering mask |
| CN105693678A (en) * | 2016-01-15 | 2016-06-22 | 甘肃寿鹿山药业有限公司 | Method for extracting procyanidine from grape seeds |
-
2016
- 2016-11-22 CN CN201611026841.5A patent/CN106726868B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1511583A (en) * | 2002-12-28 | 2004-07-14 | 石家庄制药集团制药技术开发有限公司 | Chinese rose extract and its preparing method and use |
| CN101311225A (en) * | 2007-05-26 | 2008-11-26 | 王晓明 | Process for abstracting kohlrabi pigment |
| CN102048662A (en) * | 2009-10-30 | 2011-05-11 | 爱普香料集团股份有限公司 | Method for manufacturing rose water containing natural rose pigment |
| CN103044953A (en) * | 2011-10-13 | 2013-04-17 | 黎嘉纯 | Extraction process for Chinese rose pigment |
| CN102838884A (en) * | 2012-01-18 | 2012-12-26 | 天津天康源生物技术有限公司 | Prevention method of anthocyanin pigment crystal precipitate |
| CN105106047A (en) * | 2015-08-07 | 2015-12-02 | 刘学勤 | Water-replenishing and skin-tendering mask |
| CN105693678A (en) * | 2016-01-15 | 2016-06-22 | 甘肃寿鹿山药业有限公司 | Method for extracting procyanidine from grape seeds |
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