CN106561719B - High-efficiency insecticide and fungicide containing Beauveria bassiana and pyraclostrobin and its application - Google Patents

Abstract

The invention discloses a high-efficiency insecticide and fungicide containing beauveria bassiana and pyraclostrobin, the active ingredients of which are two components of beauveria bassiana and pyraclostrobin, and the mass percentage of beauveria bassiana is 10-30 %, the mass percentage of pyraclostrobin is 10-25%. The dosage form of the insecticide is oil suspension, wettable powder or water dispersible granule. The insecticide and fungicide can be used for the prevention and control of plant diseases and insect pests, and has the effect of treating various plant diseases and insect pests such as main vegetable pest whitefly, diamondback moth and important vegetable diseases powdery mildew, downy mildew, late blight, early blight and anthracnose , and more importantly, pyraclostrobin can increase the pathogenicity of Beauveria bassiana to Plutella xylostella.

Description

High-efficiency insecticide and fungicide containing Beauveria bassiana and pyraclostrobin and its application

technical field

The invention belongs to the technical field of pesticides, and relates to an insecticidal and bactericidal composition, in particular to a high-efficiency insecticidal and bactericidal agent containing Beauveria bassiana and pyraclostrobin, which is mainly used for preventing and treating powdery mildew, downy mildew and late blight , Early blight, anthracnose, whitefly, diamondback moth and other pests and diseases.

Background technique

Beauveria bassiana is currently the most researched and applied entomopathogenic fungus in the world. It has the characteristics of strong pathogenicity, wide insecticidal spectrum, no killing of natural enemies, and ecological safety to humans and animals. For a long time, Beauveria bassiana has been widely used at home and abroad to control various agricultural and forestry pests such as grubs, whiteflies, leaf beetles, locusts, tea leafhoppers, corn borers, and pine caterpillars, and has achieved remarkable control effects. An important alternative control drug for agricultural green control system. Beauveria bassiana preparation is a living biopesticide whose effective active ingredient is conidia. When Beauveria bassiana is used to control plant pests, it will inevitably encounter various fungicides for plant disease control. The conidia of B. bassiana have different degrees of inhibitory or killing effects, resulting in different degrees of reduction in the pathogenicity of Beauveria bassiana. However, some scholars believe that the inhibition of conidial germination or growth of Beauveria bassiana does not mean that the viability of conidia must decrease.

Pyraclostrobin is a new type of broad-spectrum fungicide, the mechanism of action is mitochondrial respiration inhibitor, and it has the functions of protection, treatment and leaf penetration. All safe and friendly. Pyraclostrobin is widely used to control cucumbers, tomatoes, peppers, soybeans, peanuts, grapes, potatoes, coffee, walnuts, tea trees, tobacco, ornamental plants, lawns and other field crops caused by Ascomycetes, Basidiomycetes, and S. Diseases caused by almost all types of fungal pathogens including fungi and oomycetes. Field efficacy tests show that pyraclostrobin EC has a good effect on cucumber, watermelon powdery mildew and downy mildew, tomato and potato late blight, early blight, cabbage, cucumber, mango, tea anthracnose, corn leaf spot, etc. Control effect. Pyraclostrobin is also a plant health product, which can be used as a growth regulator, which is beneficial to crop growth, enhances crop tolerance to environmental impacts, and increases crop yield. In addition, pyraclostrobin can induce physiological changes in many crops, especially grains. For example, it can enhance the activity of nitrate (nitrification) reductase, thereby increasing the nitrogen absorption of crops during the rapid growth stage. At the same time, it can reduce the biosynthesis of ethylene and delay the senescence of crops. Even in the absence of disease in plants, pyraclostrobin can increase crop yield by controlling secondary disease and reducing stress from abiotic factors.

Pesticide compounding or mixed application technology is conducive to overcoming or delaying the resistance of pests, and can also achieve the effect of multi-directional control with one application. However, the compound use of Beauveria bassiana strains and fungicides often causes the fungicides to kill the strains, thus failing to achieve the purpose of mixed application. The inventor found that Beauveria bassiana is used in conjunction with pyraclostrobin, and pyraclostrobin not only does not kill Beauveria bassiana, but also increases the pathogenicity of Beauveria bassiana to certain pests. It is of great significance to develop insecticides and fungicides compounded with Beauveria bassiana and pyraclostrobin.

Contents of the invention

The technical problem to be solved by the present invention is to provide a high-efficiency insecticide and fungicide containing Beauveria bassiana and pyraclostrobin, wherein the pyraclostrobin can improve the pathogenicity of Beauveria bassiana to pests such as diamondback moth, It can be used to control cucumber powdery mildew, downy mildew, tomato late blight, early blight, cabbage anthracnose, whitefly, diamondback moth and other crop diseases and insect pests.

In order to achieve the above object, the present invention takes the following technical solutions:

High-efficiency insecticide and fungicide containing Beauveria bassiana and pyraclostrobin, its active ingredients are two components of Beauveria bassiana and pyraclostrobin, the mass percentage of Beauveria bassiana is 10-30%, pyrazole ether The mass percentage of strobactyl is 10-25%, and the balance is auxiliary agent.

Preferably, the Beauveria bassiana is conidia powder of Beauveria bassiana, the spore content is 5×10 ¹⁰ -1×10 ¹¹ spores/g, and the viable spore rate is >90%.

Preferably, the dosage form of the above-mentioned high-efficiency insecticide and fungicide containing Beauveria bassiana and pyraclostrobin is oil suspension, wettable powder or water dispersible granule; when the dosage form is oil suspension, the mass percentage of the auxiliary agent is: Emulsifier 2-5%, wetting agent 1-5%, antifreeze 0.5-5%, and the balance is carrier oil. Among them, the emulsifier is one of Pesticide Emulsifier 2000, Pesticide Emulsifier AE03, Pesticide Emulsifier FM or Various, the wetting agent is selected from one or more of sodium dodecylbenzene sulfonate, hydroxyethyl cellulose, sodium lauryl sulfate or sodium lignosulfonate, and the antifreeze is one of ethylene glycol or propylene glycol One or two, the carrier oil is refined mineral oil or vegetable oil; when the dosage form is wettable powder, the mass percentage of additives is: wetting agent 1-15%, dispersing agent 1-10%, and the balance is filler, of which wetting agent One or more of sodium dodecylbenzenesulfonate, sodium lauryl sulfate or sodium lignosulfonate is selected, and the dispersant is selected from one of PVP, diffusing agent MF, and alkylphenol polyoxyethylene phosphate or more, the filler is kaolin, attapulgite; when the dosage form is water-dispersible granules, the mass percentage of additives is: wetting agent 1-15%, dispersing agent 1-15%, disintegrant 0.5-4%, viscose The binder is 0.5-8%, and the balance is filler. The wetting agent is selected from one or more of sodium dodecylbenzenesulfonate, sodium lauryl sulfate or sodium lignosulfonate, and the dispersant is selected from PVP and diffusing agent. One or more of MF and alkylphenol polyoxyethylene phosphate, the disintegrant is one or more of bentonite, white carbon black, and PEG, and the binder is xanthan gum, polyvinyl alcohol, polyvinyl alcohol, One or more of sodium acrylate, and the filler is kaolin or attapulgite.

Preferably, the refined mineral oil is refined aviation kerosene or transformer oil, and the physical and chemical indicators of the refined mineral oil should meet: the relative carbon number difference of normal alkanes should not be greater than 8, and the average carbon number of relative normal alkanes should be 21-24 Between, the non-sulfonate content should not be less than 92%; the vegetable oil is corn oil or sunflower oil or rapeseed oil.

Further preferably, the Beauveria bassiana is the HFW-05 strain of Beauveria bassiana , which was deposited in the General Microorganism Center of China Committee for the Collection of Microbial Cultures on January 24, 2008, with the preservation number: CGMCCNo. 2356 , the strain has been disclosed by the invention patent application "A Beauveria bassiana HFW-05 strain and its application" (application number: 200810089529.X; publication number: CN 101245319A).

The present invention also provides the application of the high-efficiency insecticide and fungicide containing Beauveria bassiana and pyraclostrobin in the control of powdery mildew, downy mildew, late blight, early blight, anthracnose, whitefly and diamondback moth.

The advantage of the present invention is that the compound use of Beauveria bassiana and pyraclostrobin not only prevents pyraclostrobin from killing Beauveria bassiana, but also realizes the respective effects of Beauveria bassiana and pyraclostrobin at the same time. The control function improves the efficiency of treatment, and especially improves the pathogenicity of Beauveria bassiana to diamondback moth through pyraclostrobin, which brings better treatment effect. The present invention can effectively prevent and control important vegetable pests such as whitefly, diamondback moth, cucumber powdery mildew, downy mildew, tomato late blight, early blight and cabbage anthracnose, and is especially suitable for the prevention and control of green vegetable industry crop diseases and insect pests, reducing the The frequency of spraying can effectively reduce the cost of spraying for the control of crop diseases and insect pests, and achieve multiple treatments with one medicine. At the same time, it reduces the difficulty of selecting pesticides when pests and diseases occur simultaneously in vegetable green comprehensive prevention and control, which can effectively overcome or delay the resistance of whitefly, diamondback moth and other pests to chemical pesticides, and reduce the killing effect of chemical pesticides on natural enemies. Protect the ecological environment of farmland.

Detailed ways

The present invention will be further described below in conjunction with specific examples, but the examples do not limit the present invention in any form.

The percentages mentioned in the following examples are all percentages by mass, and the Beauveria bassiana is conidia powder of Beauveria bassiana, the spore content is 5×10 ¹⁰ to 1×10 ¹¹ spores/g, and the viable spore rate is >90%.

Example 1 (Preparation method of 35% Beauveria bassiana pyraclostrobin oil suspension)

1×10 ¹¹ spores/g Beauveria bassiana spore powder 10%

Pyraclostrobin 25%

Pesticide emulsifier 2000 4%

Hydroxyethyl Cellulose 2%

Ethylene glycol 1%

Refined transformer oil 58%

The preparation method is as follows:

According to the formula, weigh each component in proportion, add it to the grinder, the ambient temperature during processing is not higher than 40°C, grind for 5-10 minutes at a speed of 1000-1500rpm, and mix well.

Example 2 (Preparation method of 35% Beauveria bassiana pyraclostrobin oil suspension)

9×10 ¹⁰ spores/g Beauveria bassiana spore powder 15%

Pyraclostrobin 20%

Pesticide emulsifier AE03 4.5%

Sodium lignosulfonate 2%

Carboxyethyl Cellulose 1%

Ethylene glycol 1%

Corn Oil 56.5%

The preparation method is as follows:

According to the formula, weigh each component in proportion, add it to the grinder, the ambient temperature during processing is not higher than 40°C, grind for 5-10 minutes at a speed of 1000-1500rpm, and mix well.

Example 3 (Preparation method of 40% Beauveria bassiana pyraclostrobin wettable powder)

5×10 ¹⁰ spores/g Beauveria bassiana spore powder 30%

Pyraclostrobin 10%

Sodium dodecylbenzenesulfonate 6%

Sodium lignosulfonate 2%

Diffuser MF 5%

Kaolin 47%

Preparation:

According to the formula, each component is weighed in proportion, added to a mixer and mixed evenly, then jet milled, and fully stirred evenly after the milling is completed, and the wettable powder product can be obtained.

Example 4 (preparation method of 40% Beauveria bassiana pyraclostrobin water-dispersible granules)

1×10 ¹¹ spores/g Beauveria bassiana spore powder 20%

Pyraclostrobin 20%

Sodium dodecylbenzenesulfonate 6%

Sodium lignosulfonate 5%

Alkylphenol polyoxyethylene phosphate 5%

Polyvinyl alcohol 3%

Silica 10%

Attapulgite 31%

Preparation:

According to the formula, each component is weighed in proportion, added to a mixer to mix evenly, and then jet milled. After the milling is completed, a small amount of purified water is added to stir evenly, extruded to granulate, and dried to obtain water-dispersible granules.

Control effect:

Insecticides and fungicides containing Beauveria bassiana pyraclostrobin can effectively prevent and control the occurrence of vegetable whitefly, cucumber powdery mildew, downy mildew, tomato late blight, early blight, cabbage diamondback moth, anthracnose and other pests and diseases . As can be seen from Table 1, the control effect of this insecticide and fungicide on whitefly is equivalent to that of Beauveria bassiana, and the control effect on Plutella xylostella is better than that of Beauveria bassiana. Compared with 25% pyraclostrobin EC has the same control effect on cucumber powdery mildew, downy mildew, tomato late blight, early blight, and cabbage anthracnose, and has obvious effects on both diseases and insect pests. Farmers can control vegetable diseases and insect pests by spraying once, which reduces the workload of farmers applying pesticides. , improve the control effect on diseases and insect pests.

Table 1 Field control effect of Beauveria bassiana pyraclostrobin insecticide and fungicide after 3 applications

The above-mentioned embodiment is a preferred embodiment of the present invention, but the embodiment of the present invention is not limited by the above-mentioned embodiment, and any other changes, substitutions, combinations or simplifications made without departing from the essence and principle of the present invention, All should be equivalent replacement methods, and all are included in the protection scope of the present invention.

Claims (4)

1. A high-efficiency insecticide fungicide containing Beauveria bassiana and pyraclostrobin, characterized in that: its active ingredients are two components of Beauveria bassiana and pyraclostrobin, and the mass percentage of Beauveria bassiana is 10-30%, the mass percentage of pyraclostrobin is 10-25%, and the balance is an auxiliary agent. The Beauveria bassiana ( Beauveria bassiana ) HFW-05 strain was released in January 2008 On the 24th, it was preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the preservation number: CGMCC No. 2356. 2. the efficient insecticide bactericide containing Beauveria bassiana and pyraclostrobin as claimed in claim 1, is characterized in that, described Beauveria bassiana is Beauveria bassiana conidia powder, and spore content is 5 × 10 ¹⁰ ~1×10 ¹¹ spores/g, the viable spore rate >90%. 3. the efficient insecticide fungicide containing Beauveria bassiana and pyraclostrobin as claimed in claim 2, is characterized in that, its dosage form is oil suspension, and each component is as follows by mass percentage: 1× 10 ¹¹ spores/g Beauveria bassiana spore powder 10% Pyraclostrobin 25% Pesticide emulsifier 2000 4% Hydroxyethyl Cellulose 2% Ethylene glycol 1% Refined transformer oil 58% The preparation method is as follows: According to the formula, weigh each component in proportion, add it to the grinder, the ambient temperature during processing is not higher than 40°C, grind for 5-10 minutes at a speed of 1000-1500rpm, and mix well. 4. the high-efficiency insecticide fungicide containing Beauveria bassiana and pyraclostrobin as claimed in claim 1 is controlling powdery mildew, downy mildew, late blight, early blight, anthracnose and whitefly, diamondback moth application.