CN106561455A - Konjak interplanting method - Google Patents
Konjak interplanting method Download PDFInfo
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- CN106561455A CN106561455A CN201610959223.XA CN201610959223A CN106561455A CN 106561455 A CN106561455 A CN 106561455A CN 201610959223 A CN201610959223 A CN 201610959223A CN 106561455 A CN106561455 A CN 106561455A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention belongs to the field of agricultural plantation, and concretely relates to a konjak interplanting method. The method comprises the following steps: 1, land selecting and soil preparation; 2, konjak seedling growing; 3, konjak plantation; 4, mushroom planting; 5, field management; and 6, harvesting. The method adopting stereo planting of fruit trees, konjak and mushroom improves the output of the konjak, promotes growth of the fruit trees and increases the economic benefit.
Description
Technical field
The invention belongs to field of agricultural cultivation, and in particular to a kind of interplanting method of konjaku.
Background technology
Konjaku (Amorphophallusrivieri), You Ming Amorphophalus rivieris are Araeceae Amorphophallus herbaceos perennials.
The torrid zone and subtropical zone in Asia and Africa are mainly distributed on, the whole world there are about 170 kinds, and China there are 21 kinds.Konjaku is
One of important industrial crops, yield is high, excellent in efficiency, is the effective way that hill farmer is got rich.Konjaku is first-class vegetables,
It is also useful anoretic, contained glucomannan has functions that reducing blood lipid, hypotensive, promotion enterogastric peristalsis, to digestion, the heart
Vascular system disease and tumour have certain preventive and therapeutic action.Konjak starch or a kind of efficient adhesive, are again choosings
Ore deposit, liquefaction, printing and dyeing, the fining agent used by chemical industry.
Konjaku is single plant with compound leaves that single petiole is supported, and generally carries out vegetative propagation with underground root-like stock, but, with bulb
When making ganoid konjaku taro, its sowing quantity is larger, breeding coefficient is low and epidermis is easily damaged when preserving, transporting and sowing, easily susceptible,
Rot and lose many breedings, plus be used ball to make ganoid konjaku taro, it is sprouted, and Emerging ability is different, and the seedling of production has been difficult to meet market
Demand.
Shen Qing Publication day is on January 9th, 2013, and application publication number is the Chinese invention patent application of CN102860208A,
Disclose a kind of young economic fruit interplanting and thank to monarch's konjaku diversity cultural method, it includes that site preparation, ganoid konjaku taro are processed, sow, broadcast
Kind, field management, disease pest and weed integrated control, harvesting step, specifically include selection young economic fruit, in the ranks carry out ploughing deeply thin
Rake, selects the sowing of stripping and slicing ganoid konjaku taro, adheres to the principle of " based on base manure, farm manure, topdress, chemical fertilizer supplemented by " and carries out field management, into
Ripe harvesting.The method improves Amorphophallus Yield, promotes economic fruit from konjaku and the growth and development characteristicses of economic fruit
Growth, but, the invention does not have maximized utilization woodland soil.Shen Qing Publication day be on October 8th, 2014, application publication number
For the Chinese patent application of CN104082005A, a kind of intercropping culture method of konjaku and ambrette is disclosed, step includes that (1) is whole
Ground ridging；(2) okra nursery；(3) konjaku sowing；(4) plant；(5) field management；(6) okra harvesting；(7) konjaku is adopted
Receive.Konjaku belongs to the shady crop of happiness, and the yield and quality of konjaku can be improved under certain shading condition, and okra is happiness sun
The crop of light, makees just to take what one needs between okra and konjaku, effectively utilizes sunlight.But, deficiency is there is also in the present invention,
Most of Root Distributions of konjaku are in earth's surface 25cm or so, and the root system of okra is between 20～40cm, therefore, konjaku and Huang
Make well absorb the moisture and nutrient in different depth soil between certain herbaceous plants with big flowers.
Accordingly, it is now desired to a kind of method comes maximized raising woodland land utilization ratio and different depth soil moisture
With the utilization of nutrient.
The content of the invention
It is an object of the invention to provide a kind of interplanting method of konjaku, for solving konjaku growth happiness the moon, woodland soil profit
With and different depth soil horizon in moisture and nutrien utilization problem.
In order to solve above-mentioned technical problem, the technical scheme is that：
A kind of interplanting method of konjaku, comprises the steps：
S1, selection of land and site preparation
Two fruit tree the forest lands more than age are selected to interplant land used as konjaku, it is desirable to which the soil is porous, rich in organic matter, pH
6.5～7.5 (acid-base value of soil affects larger to Amorphophallus Yield), are in the winter time ploughed deeply timbered soil, after spring has set in,
40～50cm is spaced in forest land and excavates wide 20cm, the ditch of deep 30～50cm, as the plantation ditch of konjaku；Konjaku is ombrophyte,
Intense light irradiation is not liked；Fruit tree woods can provide the environment for sheltering from heat or light for the growth of konjaku, while the fallen leaves of fruit tree can also be konjaku
Growth provide humus；When konjaku is planted, forest land is turned over, increased the throughput of soil, increased fruit tree root
The breathing in portion, improves output of the fruit tree.
S2, konjaku nursery
1) culture medium prepares：Callus inducing medium：MS+1.0mg/L NAA+1.0mg/L 6-BA+3g/L sucrose+
5.0g/L agar+0.2mg/L matrines, adjust pH 6.0～7.0；Callus differential medium：MS+1.0mg/L NAA+
0.1mg/L 6-BA+3g/L sucrose+5.0g/L agar+0.5mg/L PVP+5mg/L gentamicins, adjust pH 6.0～7.0；Take root
Inducing culture：MS+1.0mg/L NAA+1.0mg/L 6-BA+3g/L sucrose+5.0g/L agar+0.05mg/L GA3+
0.1mg/L matrines, adjust pH 6.0～7.0；Through the present inventor discovery pH 6.0～7.0 is studied for a long period of time for konjaku group
Knit the optimal pH of culture.
2) explant prepares：Take konjaku terminal bud and do explant, terminal bud is soaked into 60s in 75% ethanol, then 0.2%
30min is soaked in HgCl solution, with aseptic water washing 5～8 times；Then, terminal bud is cut into the fritter of 5mm*5mm*5mm；
3) it is inoculated with：Ready explant is seeded on callus inducing medium, under conditions of 20～25 DEG C
Light culture 5～10 days, then cultivates 35～40 days under conditions of 20～25 DEG C, 1500～2000Lx, daily illumination 12 hours,
Obtain callus；
4) squamous subculture：Above-mentioned callus is transferred on callus differential medium, 20～25 DEG C, 1500～
Cultivate under conditions of 1800Lx, daily illumination 12 hours, proliferation times are 1 per 10～15 days subcultures once:5～8；
5) culture of rootage：The seedling that squamous subculture is obtained is transferred on root induction culture medium, 20～25 DEG C, 1800
Cultivate 7～15 days under conditions of～2000Lx, daily illumination 12 hours；
6) hardening：Seedling after taking root takes out, and cleans the culture medium of root, is transplanted in the sandy seedbed in greenhouse,
Natural lighting, hardening 3～7 days, obtains konjaku seedling；Selecting the mode of tissue cultures carries out konjaku breeding, solves konjaku plantation
During, the big problem of ganoid konjaku taro consumption.
S3, konjaku plantation
The Nutrition Soil of 15～35cm of thick layer is backfilled in the plantation ditch for excavating in step sl, thick 8 are covered on Nutrition Soil
The hay of～10cm, then the Nutrition Soil of thickness 5cm is covered on hay, by konjaku Seeding planting in plantation ditch, spacing in the rows 40～
50cm, pours root water；
S4, mushroom sowing
When konjaku length to more than 20cm, after mushroom-cultivating bacterial classification is well mixed with culture matrix, two row konjakus it
Between make fabric width for 30cm, the plantation ridge of a height of 20cm, now, into ditch between two plantation ridges, konjaku is located in described ditch,
One layer of thin soil is covered on the face of ridge, then covers one layer of straw, water drenched to plantation ridge, and keep moist；The growth of mushroom is not required to
Want sunlight and do not like high light, happiness humidity, be planted in and konjaku interplanting, konjaku plant can provide sunshade, tide for mushroom
Wet environment；Remaining culture matrix also provides nutrient for konjaku after mushroom harvesting.
S5, field management
Intertillage fertilising is carried out in the phase of lifting of konjaku growth, in every plant of 3～5g of potassium application, the calcium magnesium phosphate 8 away from taro strain 5cm
～10g, is covered with ditch soil；Expanding stage is grown in konjaku, the thick farm manures of 5～10cm are applied into furrow, then in farm manure overlying
3～5cm of soil, pours permeable；
Cannot directly spray water pouring when mushroom fruiting is buddingged, by implementing to irrigate to pouring water between two plantation ridges,
The effect irrigated the mushroom in plantation ridge is reached by the capillarity of soil while irrigation konjaku, in dry weather
When, also the plantation ridge of mushroom is sprayed, to keep with the humid air；In the growth course of whole konjaku, weeds are found
To pull out by hand in time；
S6, harvesting
Harvest in time before mushroom parachute-opening, mushroom can be harvested to before entering the winter, konjaku plantation is not harvested then, and maternal plant yellow is fallen
In after seedling and mushroom plantation ridge no longer after fruiting, plantation ridge is scooped up, is smashed to pieces, being covered to konjaku plantation ditch；Next year, original
Fabric width reform for 30cm, the plantation ridge of a height of 20cm in the position for doing plantation ridge；After seedling is fallen in next year konjaku maternal plant yellow carries out konjaku
Harvesting.
In the interplanting method of the konjaku that the present invention is provided, it is preferable that the weight ratio of the Nutrition Soil composition is garden mould：Grass
Wood ash：Sheep excrement：Slow-release fertilizer=4:1:2:1；The weight ratio of the Nutrition Soil composition can also be garden mould：Plant ash：Sheep excrement：Ox
Excrement：Slow-release fertilizer=4:1:2:1:1；Described sheep excrement is the sheep excrement become thoroughly decomposed；The cow dung is the cow dung become thoroughly decomposed；Described slow-release fertilizer
For bar-shaped slow-release fertilizer, the raw material of the bar-shaped slow-release fertilizer consisting of by weight：10～18 parts of potassium chloride, potassium dihydrogen phosphate 10
3～5 parts of～20 parts, 5～10 parts of Humic acid Magnesium, 3～5 parts of zinc sulfate and copper sulphate.
In the interplanting method of the konjaku that the present invention is provided, it is preferable that the weight ratio of the culture matrix composition is garden mould：
Cotton seed hulls：Wood chip：Corncob：Cutted straw：Cow dung：Chicken manure：Lime：Calcium superphosphate=5:1:3:2:1:1:1:0.2:0.2；It is described
It is the cow dung become thoroughly decomposed that cow dung is the cow dung become thoroughly decomposed；The chicken manure is the chicken manure for becoming thoroughly decomposed.
In the interplanting method of the konjaku that the present invention is provided, it is preferable that the fruit tree is pear tree or walnut；Further preferably
Ground, the fruit tree is walnut, and the optimum pH of walnut growth is with the optimum pH of konjaku growth relatively.
In the interplanting method of the konjaku that the present invention is provided, it is preferable that the konjaku is white konjaku or elephant-foot yam；Further
Preferably, the konjaku is elephant-foot yam.
In the interplanting method of the konjaku that the present invention is provided, it is preferable that the mushroom is coprinus comatus.
Using technical scheme, due to fruit tree, konjaku and mushroom being interplanted, solid growth is realized；Evil spirit
Taro is ombrophyte, does not like intense light irradiation；Fruit tree woods can provide the environment for sheltering from heat or light for the growth of konjaku, while the fallen leaves of fruit tree
Humus can also be provided for the growth of konjaku；When konjaku is planted, forest land is turned over, increased the throughput of soil,
The breathing of fruit tree root is increased, output of the fruit tree is improve.The growth of mushroom does not need sunlight and does not like high light, happiness humidity,
It is planted in and konjaku interplanting, konjaku plant can provide sunshade, moist environment for mushroom；Remaining training after mushroom harvesting
Foster matrix also provides nutrient for konjaku.Selecting the mode of tissue cultures carries out konjaku breeding, in solving konjaku planting process, plants
The big problem of taro consumption.
Specific embodiment
The specific embodiment of the present invention is described further below.Here is it should be noted that for these enforcements
The explanation of mode is used to help understand the present invention, but does not constitute limitation of the invention.Additionally, invention described below
As long as involved technical characteristic does not constitute each other conflict and just can be mutually combined in each embodiment.
Embodiment 1
A kind of interplanting method of konjaku, comprises the steps：
S1, selection of land and site preparation
Two the walnut forest lands more than age are selected to interplant land used as konjaku, it is desirable to which the soil is porous, rich in organic matter, pH
6.5～7.5 (acid-base value of soil affects larger to Amorphophallus Yield), are in the winter time ploughed deeply timbered soil, after spring has set in,
40～50cm is spaced in forest land and excavates wide 20cm, the ditch of deep 30～50cm, as the plantation ditch of konjaku；
S2, konjaku nursery
1) culture medium prepares：Callus inducing medium：MS+1.0mg/L NAA+1.0mg/L 6-BA+3g/L sucrose+
5.0g/L agar+0.2mg/L matrines, adjust pH 6.2；Callus differential medium：MS+1.0mg/L NAA+0.1mg/L
6-BA+3g/L sucrose+5.0g/L agar+0.5mg/L PVP+5mg/L gentamicins, adjust pH 6.5；Root induction culture medium：MS
+ 1.0mg/L NAA+1.0mg/L 6-BA+3g/L sucrose+5.0g/L agar+0.05mg/L GA3+0.1mg/L matrines, adjust pH
6.2；
2) explant prepares：Take konjaku terminal bud and do explant, terminal bud is soaked into 60s in 75% ethanol, then 0.2%
30min is soaked in HgCl solution, with aseptic water washing 5 times；Then, terminal bud is cut into the fritter of 5mm*5mm*5mm；
3) it is inoculated with：Ready explant is seeded on callus inducing medium, under conditions of 20～25 DEG C
Light culture 10 days, then cultivates 35 days under conditions of 20～25 DEG C, 1500Lx, daily illumination 12 hours, obtains callus；
4) squamous subculture：Above-mentioned callus is transferred on callus differential medium, 20～25 DEG C,
Cultivate under conditions of 1800Lx, daily illumination 12 hours, proliferation times are 1 per 15 days subcultures once:5；
5) culture of rootage：The seedling that squamous subculture is obtained is transferred on root induction culture medium, 20～25 DEG C,
Cultivate 8 days under conditions of 1800Lx, daily illumination 12 hours；
6) hardening：Seedling after taking root takes out, and cleans the culture medium of root, is transplanted in the sandy seedbed in greenhouse,
Natural lighting, hardening 5 days, obtains konjaku seedling；
S3, konjaku plantation
The Nutrition Soil of 15～35cm of thick layer is backfilled in the plantation ditch for excavating in step sl, cover on Nutrition Soil it is thick by 8～
The hay of 10cm, then the Nutrition Soil of thickness 5cm is covered on hay, by konjaku Seeding planting in plantation ditch, 40～50cm of spacing in the rows,
Pour root water；The weight ratio of Nutrition Soil composition is garden mould：Plant ash：Sheep excrement：Slow-release fertilizer=4:1:2:1；Slow-release fertilizer is bar-shaped
Slow-release fertilizer, its raw material consisting of by weight：10 parts of potassium chloride, 20 parts of potassium dihydrogen phosphate, 8 parts of Humic acid Magnesium, 3 parts of zinc sulfate
With 5 parts of copper sulphate.
S4, mushroom sowing
When konjaku length is to more than 20cm, after cultivation bacterial classification is well mixed with culture matrix, between two row konjakus
It is 30cm, the plantation ridge of a height of 20cm to make fabric width, and now, into ditch between two plantation ridges, konjaku is located in ditch, in ridge face overlying
One layer of thin soil of lid, then one layer of straw is covered, water drenched to plantation ridge, and keep moist；The weight ratio of culture matrix composition is
Garden mould：Cotton seed hulls：Wood chip：Corncob：Cutted straw：Cow dung：Chicken manure：Lime：Calcium superphosphate=5:1:3:2:1:1:1:0.2:
0.2。
S5, field management
Intertillage fertilising is carried out in the phase of lifting of konjaku growth, in every plant of potassium application 5g, the calcium magnesium phosphate 8g away from taro strain 5cm,
Covered with ditch soil；Grow expanding stage in konjaku, apply the thick farm manures of 5～10cm into furrow, then on farm manure earthing 3～
5cm, pours permeable；
Cannot directly spray water pouring when mushroom fruiting is buddingged, by implementing to irrigate to pouring water between two plantation ridges,
The effect irrigated the mushroom in plantation ridge is reached by the capillarity of soil while irrigation konjaku, in dry weather
When, also the plantation ridge of mushroom is sprayed, to keep with the humid air；In the growth course of whole konjaku, weeds are found
To pull out by hand in time；
S6, harvesting
Harvest in time before mushroom parachute-opening, mushroom can be harvested to before entering the winter, konjaku plantation is not harvested then, and maternal plant yellow is fallen
After seedling and mushroom plantation ridge no longer fruiting, plantation ridge is scooped up, is smashed to pieces, being covered to konjaku plantation ditch；Next year, plant originally
Fabric width reform for 30cm, the plantation ridge of a height of 20cm in the position for planting ridge；After seedling is fallen in next year konjaku maternal plant yellow carries out adopting for konjaku
Receive.
The konjaku selected in the present embodiment is elephant-foot yam, mushroom is coprinus comatus.
Embodiment 2
A kind of interplanting method of konjaku, comprises the steps：
S1, selection of land and site preparation
Two pear tree the forest lands more than age are selected to interplant land used as konjaku, it is desirable to which the soil is porous, rich in organic matter, pH
6.5～7.5 (acid-base value of soil affects larger to Amorphophallus Yield), are in the winter time ploughed deeply timbered soil, after spring has set in,
40～50cm is spaced in forest land and excavates wide 20cm, the ditch of deep 30～50cm, as the plantation ditch of konjaku；
S2, konjaku nursery
1) culture medium prepares：Callus inducing medium：MS+1.0mg/L NAA+1.0mg/L 6-BA+3g/L sucrose+
5.0g/L agar+0.2mg/L matrines, adjust pH7.0；Callus differential medium：MS+1.0mg/L NAA+0.1mg/L 6-
BA+3g/L sucrose+5.0g/L agar+0.5mg/L PVP+5mg/L gentamicins, adjust pH 6.0；Root induction culture medium：MS+
1.0mg/L NAA+1.0mg/L 6-BA+3g/L sucrose+5.0g/L agar+0.05mg/L GA3+0.1mg/L matrines, adjust pH
7.0；
2) explant prepares：Take konjaku terminal bud and do explant, terminal bud is soaked into 60s in 75% ethanol, then 0.2%
30min is soaked in HgCl solution, with aseptic water washing 8 times；Then, terminal bud is cut into the fritter of 5mm*5mm*5mm；
3) it is inoculated with：Ready explant is seeded on callus inducing medium, under conditions of 20～25 DEG C
Light culture 5 days, then cultivates 40 days under conditions of 20～25 DEG C, 2000Lx, daily illumination 12 hours, obtains callus；
4) squamous subculture：Above-mentioned callus is transferred on callus differential medium, 20～25 DEG C,
Cultivate under conditions of 1500Lx, daily illumination 12 hours, proliferation times are 1 per 10 days subcultures once:8；
5) culture of rootage：The seedling that squamous subculture is obtained is transferred on root induction culture medium, 20～25 DEG C,
Cultivate 15 days under conditions of 2000Lx, daily illumination 12 hours；
6) hardening：Seedling after taking root takes out, and cleans the culture medium of root, is transplanted in the sandy seedbed in greenhouse,
Natural lighting, hardening 7 days, obtains konjaku seedling；
S3, konjaku plantation
The Nutrition Soil of 15～35cm of thick layer is backfilled in the plantation ditch for excavating in step sl, thick 8 are covered on Nutrition Soil
The hay of～10cm, then the Nutrition Soil of thickness 5cm is covered on hay, by konjaku Seeding planting in plantation ditch, spacing in the rows 40～
50cm, pours root water；The weight ratio of Nutrition Soil composition is garden mould：Plant ash：Sheep excrement：Cow dung：Slow-release fertilizer=4:1:2:1:1；
Slow-release fertilizer is bar-shaped slow-release fertilizer, its raw material consisting of by weight：18 parts of potassium chloride, 12 parts of potassium dihydrogen phosphate, Humic acid Magnesium
3 parts of 10 parts, 5 parts of zinc sulfate and copper sulphate.
S4, mushroom sowing
When konjaku length is to more than 20cm, after cultivation bacterial classification is well mixed with culture matrix, between two row konjakus
It is 30cm, the plantation ridge of a height of 20cm to make fabric width, now, into ditch between two plantation ridges, in the ditch described in konjaku umbrella shape, on ridge
One layer of thin soil is covered on face, then covers one layer of straw, water drenched to plantation ridge, and keep moist；The weight of culture matrix composition
Amount is than being garden mould：Cotton seed hulls：Wood chip：Corncob：Cutted straw：Cow dung：Chicken manure：Lime：Calcium superphosphate=5:1:3:2:1:1:1:
0.2:0.2。
S5, field management
Intertillage fertilising is carried out in the phase of lifting of konjaku growth, in every plant of potassium application 3g, the calcium magnesium phosphate 10g away from taro strain 5cm,
Covered with ditch soil；Grow expanding stage in konjaku, apply the thick farm manures of 5～10cm into furrow, then on farm manure earthing 3～
5cm, pours permeable；
Cannot directly spray water pouring when mushroom fruiting is buddingged, by implementing to irrigate to pouring water between two plantation ridges,
The effect irrigated the mushroom in plantation ridge is reached by the capillarity of soil while irrigation konjaku, in dry weather
When, also the plantation ridge of mushroom is sprayed, to keep with the humid air；In the growth course of whole konjaku, weeds are found
To pull out by hand in time；
S6, harvesting
Harvest in time before mushroom parachute-opening, mushroom can be harvested to before entering the winter, konjaku plantation is not harvested then, and maternal plant yellow is fallen
On seedling and mushroom plantation ridge no longer after fruiting, plantation ridge is scooped up, is smashed to pieces, being covered to konjaku plantation ditch；Next year, do originally
Fabric width reform for 30cm, the plantation ridge of a height of 20cm in the position of plantation ridge；After seedling is fallen in next year konjaku maternal plant yellow carries out konjaku
Harvesting.
Konjaku selected in the present embodiment is elephant-foot yam, mushroom is coprinus comatus.
Embodiments of the present invention are explained in detail above, but the invention is not restricted to described embodiment.It is right
For those skilled in the art, in the case of without departing from the principle of the invention and spirit, these embodiments are carried out many
Plant change, modification, replace and modification, still fall within protection scope of the present invention.
Claims (6)
1. a kind of interplanting method of konjaku, it is characterised in that comprise the steps：
S1, selection of land and site preparation
Select two fruit tree the forest lands more than age as konjaku interplant land used, it is desirable to the soil is porous, rich in organic matter, pH6.5～
7.5, timbered soil is ploughed deeply in the winter time, after spring has set in, be spaced in forest land 40～50cm excavate wide 20cm, deep 30～
The ditch of 50cm, as the plantation ditch of konjaku；
S2, konjaku nursery
1) culture medium prepares：Callus inducing medium：MS+1.0mg/L NAA+1.0mg/L6-BA+3g/L sucrose+5.0g/
L agar+0.2mg/L matrines, adjust pH 6.0～7.0；Callus differential medium：MS+1.0mg/L NAA+0.1mg/L
6-BA+3g/L sucrose+5.0g/L agar+0.5mg/L PVP+5mg/L gentamicins, adjust pH 6.0～7.0；Root induction culture
Base：MS+1.0mg/L NAA+1.0mg/L 6-BA+3g/L sucrose+5.0g/L agar+0.05mg/L GA3+0.1mg/L kuh-sengs
Alkali, adjusts pH 6.0～7.0；
2) explant prepares：Take konjaku terminal bud and do explant, terminal bud is soaked into 60s in 75% ethanol, then in 0.2%HgCl
30min is soaked in solution, with aseptic water washing 5～8 times；Then, terminal bud is cut into the fritter of 5mm*5mm*5mm；
3) it is inoculated with：Ready explant is seeded on callus inducing medium, is secretly trained under conditions of 20～25 DEG C
Support 5～10 days, then cultivate 35～40 days under conditions of 20～25 DEG C, 1500～2000Lx, daily illumination 12 hours, obtain more
Injured tissue；
4) squamous subculture：Above-mentioned callus is transferred on callus differential medium, 20～25 DEG C, 1500～
Cultivate under conditions of 1800Lx, daily illumination 12 hours, proliferation times are 1 per 10～15 days subcultures once:5～8；
5) culture of rootage：The seedling that squamous subculture is obtained is transferred on root induction culture medium, 20～25 DEG C, 1800～
Cultivate 7～15 days under conditions of 2000Lx, daily illumination 12 hours；
6) hardening：Seedling after taking root takes out, and cleans the culture medium of root, is transplanted in the sandy seedbed in greenhouse, natural
Illumination, hardening 3～7 days, obtains konjaku seedling；
S3, konjaku plantation
The Nutrition Soil of 15～35cm of thick layer is backfilled in the plantation ditch for excavating in step sl, 8～10cm of thickness is covered on Nutrition Soil
Hay, then on hay cover thickness 5cm Nutrition Soil, by konjaku Seeding planting in plant ditch in, 40～50cm of spacing in the rows is poured
Root water；
S4, mushroom sowing
When konjaku length to more than 20cm, after mushroom-cultivating bacterial classification is well mixed with culture matrix, do between two row konjakus
Into the plantation ridge that fabric width is 30cm, a height of 20cm, one layer of thin soil is covered on the face of ridge, then cover one layer of straw, watered to plantation
Ridge drenches, and keeps moist；
S5, field management
Carry out intertillage fertilising in the phase of lifting of konjaku growth, every plant of 3～5g of potassium application away from taro strain 5cm, calcium magnesium phosphate 8～
10g, is covered with ditch soil；Expanding stage is grown in konjaku, the thick farm manures of 5～10cm, then the earthing on farm manure are applied into furrow
3～5cm of thickness, pours permeable；
Cannot directly spray water pouring when mushroom fruiting is buddingged, by implementing to irrigate to pouring water between two plantation ridges, to evil spirit
The effect irrigated the mushroom in plantation ridge is reached by the capillarity of soil while taro is irrigated, in dry weather, also
The plantation ridge of mushroom is sprayed, to keep with the humid air；In the growth course of whole konjaku, it is found that weeds are timely
Pull out by hand；
S6, harvesting
Harvest in time before mushroom parachute-opening, konjaku is not harvested then in plantation, after seedling is fallen in next year maternal plant yellow carries out the harvesting of konjaku.
2. the interplanting method of konjaku according to claim 1, it is characterised in that the weight ratio of the Nutrition Soil composition is,
Garden mould:Plant ash:Sheep excrement:Slow-release fertilizer=4:1:2:1.
3. the interplanting method of konjaku according to claim 1, it is characterised in that the weight ratio of the culture matrix composition
For garden mould:Cotton seed hulls:Wood chip:Corncob:Cutted straw:Cow dung:Chicken manure:Lime:Calcium superphosphate=5:1:3:2:1:1:1:0.2:
0.2。
4. the interplanting method of konjaku according to claim 1, it is characterised in that the fruit tree is pear tree or walnut.
5. the interplanting method of konjaku according to claim 1, it is characterised in that the konjaku is elephant-foot yam.
6. the interplanting method of konjaku according to claim 1, it is characterised in that the mushroom is coprinus comatus.
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