CN106377338A - Syringe needle head and use method of syringe - Google Patents

Abstract

The invention provides a syringe needle head and a use method of a syringe. The syringe needle head comprises a needle stalk front section and a needle stalk tail section which are mutually separated; the first end of the needle stalk front section is a needle head; the first end of the needle stalk tail section is connected with a pintle; the syringe needle head also comprises a transparent soft catheter; one end of the soft catheter is butted with the second end of the needle stalk front section; the other end of the soft catheter is butted with the second end of the needle stalk tail section; inner diameters of two ends of the soft catheter are not greater than the outer diameter of the needle stalk. When the syringe needle head and the use method of the syringe provided by the embodiment of the invention are adopted for a mouse tail vein injection experiment, if needle insertion is successful, a blood return phenomenon can be found by the transparent soft catheter, so that the reliability of the mouse tail vein injection experiment is improved.

Description

A syringe needle and a method for using the syringe

technical field

The invention relates to the technical field of experimental equipment and methods, in particular to a stable and efficient syringe needle and a method for using the syringe.

Background technique

In all kinds of basic medical research, in order to observe the pharmacodynamics and toxicology of drugs, new compounds, etc., or in the etiology, pathogenesis, and pathological research of diseases, in order to observe the function, metabolism, and morphological changes of animals, it is necessary to carry out In animal experiments, a certain method is used to administer drugs to the animals to be tested. And mouse tail vein injection is one of the most commonly used ways of administration. It is the premise of using the tail vein injection method to formulate accurate and fast single-person operation method and related equipment.

Tail vein injection of mice is one of the commonly used methods of drug administration in animal experiments, but the drug administration process has the disadvantages of difficult operation and low success rate. The operation process of the traditional mouse tail vein injection method is as follows: fix the mouse with a mouse fixer; straighten the mouse tail, wipe the mouse tail with alcohol to fully expand the vein; use a syringe with a needle (usually No. Insert the needle at the distal end of the tail (generally 1/4 or 1/3 from the tip of the tail). If the needle advances in the blood vessel, you can obviously feel the needle travel smoothly. There is no resistance when trying to push the liquid, which means that the injection is successful; otherwise If it fails, the needle needs to be inserted again (move to the proximal injection point and try again). Since the blood vessel will shrink after being stimulated, it will increase the difficulty of injection. Therefore, if the same vein fails three times, take a short rest and switch to another vein. injection.

In the above experimental process, when the syringe is directly injected into the tail vein, the blood return phenomenon cannot be used to judge whether the needle is inserted successfully.

Contents of the invention

In view of this, the embodiment of the present invention provides a stable and efficient method for using a syringe needle and a syringe, so as to improve the reliability of the mouse tail vein experiment.

In order to achieve the above purpose, embodiments of the present invention provide the following technical solutions:

A syringe needle, comprising: a needle plug and a needle barrel, the needle barrel includes: a front section of the needle barrel and a tail section of the needle barrel, which are independent of each other, the first end of the front section of the needle barrel is a needle, and the tail section of the needle barrel the first end is connected to the pintle;

The syringe needle also includes: a transparent flexible conduit, one end of the flexible conduit is docked with the second end of the front section of the needle barrel, the other end of the flexible conduit is docked with the second end of the tail section of the needle barrel, The inner diameters at both ends of the soft catheter are not greater than the outer diameter of the needle barrel.

Preferably, for the above-mentioned syringe needle, the inner diameter of the flexible catheter is not greater than 0.28mm.

Preferably, in the above-mentioned syringe needle, the outer diameter of the needle barrel is not greater than 0.3mm.

Preferably, for the above-mentioned syringe needle, one end of the flexible conduit is sleeved on the second end of the front section of the needle barrel, and the other end of the flexible conduit is sleeved on the second end of the tail section of the needle barrel.

A method for using a syringe, which is applied in a mouse tail vein injection experiment, the syringe is equipped with any one of the disclosed syringe needles above, and the method includes:

The needle tip at the front section of the needle barrel is used to insert the needle at the injection point, wherein the injection point is: the injection point on the tail vein of the mouse that is tightened at a preset distance up and down; the vein is: Straighten, wipe with alcohol swab until the vein is dilated and facing the user's vein on the tail side of the mouse;

Operate the syringe for dosing or cell injection.

Preferably, in the method for using the above-mentioned syringe, the needle tip of the front section of the needle barrel is used to insert the needle at the injection point, including:

Keep the angle between the front section of the needle barrel and the tail of the mouse within the preset angle range, use the needle tip of the front section of the needle barrel to insert the needle at the injection point, and then insert the needle in parallel after a set distance.

Preferably, in the above method of using the syringe, the preset angle range is: not less than 10°, not more than 20°.

Preferably, in the method for using the above-mentioned syringe, the needle tip of the front section of the needle barrel is used to insert the needle at the injection point, including:

Use straight-toothed tweezers to fix the 1/3 position from the second end of the front section of the needle barrel, use the needle tip of the front section of the needle barrel to insert the needle at the injection point, and insert the needle in parallel after a set distance.

Preferably, in the method for using the above-mentioned syringe, the mouse is an anesthetized living mouse.

Preferably, in the above method of using the syringe, the preset distance is 1 cm.

Based on the above-mentioned technical scheme, when using the syringe needle provided in the embodiment of the present invention and the use method of the syringe to carry out the mouse tail vein injection experiment, when the needle is inserted successfully, the phenomenon of blood return can be found through the transparent soft catheter, thus improving the blood flow rate of the mouse. Reliability of tail vein injection experiments.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only It is an embodiment of the present invention, and those skilled in the art can also obtain other drawings according to the provided drawings without creative work.

Fig. 1 is a schematic structural view of a syringe pillow disclosed in an embodiment of the present application;

Fig. 2 is a flow chart of a method for using a syringe disclosed in an embodiment of the present application.

detailed description

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

The applicant conducted research on the experimental methods in the prior art and found that the reason why the blood return phenomenon could not be found during the mouse tail vein injection experiment in the prior art is that the mouse tail vein injection experiment was performed directly using a syringe + a conventional syringe needle At this time, since the conventional syringe needle is an opaque metal needle, if you want to find the blood return phenomenon, you must enter the syringe in large quantities after the tail vein return blood of the mouse flows through the conventional syringe needle. The author found that, however, due to the very low blood pressure in the tail vein of the mouse and the limited amount of blood flowing in the tail vein of the mouse, it was not enough for the return blood of the tail vein to flow into the syringe. The method of venous experiment cannot find the phenomenon of tail vein blood return, which leads to low reliability of the experiment.

In view of the above research, the applicant has invented a syringe needle for improving the reliability of intravenous injection to solve the problem of low reliability during tail vein injection of mice, and during the tail vein injection of mice, the How to use the syringe.

Referring to Fig. 1, the syringe needle disclosed in the embodiment of the present application includes: a needle plug 100, a needle barrel 200 and a transparent soft catheter 300, and the needle barrel 200 includes: a needle barrel front section 201 and a needle barrel tail section 202, which are independent of each other, The first end of the front section 201 of the needle barrel is a needle head, the first end of the tail section 202 of the needle barrel is connected to the pintle 100; one end of the flexible catheter 300 is connected to the second end of the front section 201 of the needle barrel The other end of the soft catheter 300 is docked with the second end of the needle barrel tail section 202, and the inner diameters of both ends of the soft catheter 300 are not greater than the outer diameter of the needle barrel 200, so as to achieve no leakage between the two. Missing butt.

When using the syringe needle pair disclosed in the above embodiments of the present application for tail vein injection of mice, since the needle barrel 200 is divided into two sections, the front section of the needle barrel 201 and the tail section of the needle barrel 202, and the two sections of the needle barrel It is connected by a transparent soft catheter. When the mouse tail vein is injected, as long as the mouse tail vein blood flows into the soft catheter, the user can find the phenomenon of blood return. By setting the inner diameter of the soft catheter reasonably, few Therefore, it can be seen that the mouse tail vein experiment carried out using the syringe needle disclosed in the embodiment of the present application can judge whether the needle is inserted successfully by judging the blood return of the tail vein of the mouse. Thereby improving the reliability of the mouse tail vein experiment.

Wherein, when selecting the soft catheter 300, in order to use as little mouse tail vein blood as possible to find the blood return phenomenon, the inner diameter of the soft catheter 300 should be as small as possible. In this case, the inner diameter of the soft conduit 300 should not be greater than 28mm, and its length can be selected according to the needs. For example, for the convenience of experiments, the length can be 8-10cm.

Except that the inner diameter of the soft catheter 300 affects the amount of blood in the tail vein of the mouse used when the phenomenon of blood return is found, the inner diameter of the needle plug 200 and the length of the front section 201 of the needle plug also affect the amount of blood used when the phenomenon of blood return is found. Therefore, when selecting the pintle 200, the inner diameter of the pintle 200 should be as thin as possible, and the front section 201 of the pintle should be as short as possible without affecting the needle insertion, for example, The length of the front section 201 of the needle barrel is preferably not greater than 1.25 cm.

Further, in order to ensure that there is no leakage between the soft catheter and the needle barrel 200, in the above embodiment, one end of the soft catheter 300 is sleeved on the second end of the front section 201 of the needle barrel, and the soft catheter 300 The other end of the sleeve is sleeved on the second end of the needle barrel tail section 202, and the sleeve length ratio is less than 2mm without affecting the needle insertion.

Regarding the above-mentioned syringe needle, the present application also discloses a method for using the syringe used in the mouse tail vein injection experiment, the syringe is equipped with the syringe needle disclosed in any one of the above-mentioned embodiments of the present application, see Figure 2, Methods include:

Step S101: using the needle tip at the front section of the needle barrel to insert a needle at the injection point;

Wherein, the injection point is: the injection point on the tail vein of the mouse that is tightened at the preset distance up and down; The vein on the side end of the rat tail, preferably, in order to ensure smooth insertion of the needle, the preset distance is 1cm;

Step S102: Operate the syringe to perform quantitative drug administration or cell injection.

When carrying out the above method of using the syringe, it is possible to find the phenomenon of back blood in the tail vein of the mouse, thereby improving the reliability of the tail vein injection of the mouse.

The process of carrying out the mouse tail vein injection experiment using the above-mentioned syringe method includes:

Step 1. Preparation of experimental materials;

Step 2. Straighten the tail of the mouse and deflect it slightly (because the tail vein of the mouse is on both sides of the tail), wipe the tail vein with alcohol cotton to see its expansion, fix and tighten the tail about 1cm above and below the injection point (you can use the left index finger and middle finger to finish);

Of course, in this step, in order to prevent the mouse from moving and increase the difficulty of needle insertion, the mice used in the mouse tail vein injection experiment and the mice used in the method of using the syringe are all anesthetized mice.

Step 3, inserting the needle;

Of course, in order to ensure the success rate of needle insertion, the present application also defines the needle insertion step, that is, in the above method of using the syringe, the step S101 may specifically include: keeping the front section of the needle barrel in contact with the tail of the mouse. When the angle is within the preset angle range, the needle tip at the front section of the needle barrel is used to insert the needle at the injection point, and the needle is inserted in parallel after a set distance. Wherein, the preset angle range is an interval of [10°, 20°]. Preferably, the angle between the front section of the needle stem and the tail of the mouse is 15°.

During the use of the above-mentioned syringe, in addition to limiting the needle insertion angle, the way of holding the needle is also limited, that is, the step S101 may specifically include: using straight toothed forceps to fix the second end of the front section of the needle barrel from 1/ At position 3, use the needle tip at the front section of the needle barrel to insert the needle at the injection point, and then advance the needle parallel to the set distance.

Of course, in the method disclosed in the embodiment of the present application, the further optimal solution of step S101 is: use straight toothed forceps to fix the position at 1/3 of the second end of the front section of the needle barrel, and keep the front section of the needle barrel in contact with the The angle of the tail of the mouse is within the preset angle range, and the needle tip of the front section of the needle barrel is used to insert the needle at the injection point, and then the needle is inserted in parallel after a set distance.

When performing mouse tail vein injection, the needle insertion process can specifically be: use the right thumb and forefinger to fix and control the straight tweezers (self-made injection needle) at the position of 1/3 of the front section of the needle plug from the second end, at the Insert the needle at 1/4 or 1/3 of the tail tip of the tail and the vein at 15°. After inserting the needle for a certain set distance, insert the needle in parallel. If blood returns to the front end of the soft catheter, it means that the needle is inserted successfully;

Step 4. Fix the injection needle with the right hand and operate the syringe with the left hand to perform quantitative drug administration or cell injection;

Step 5. After the injection is completed, take a dry cotton swab and press the injection point after quickly pulling out the needle to avoid backflow or bleeding of the injection.

Compared with the currently commonly used mouse tail vein injection experiment, the mouse tail vein injection experiment carried out by using the syringe method disclosed in the above-mentioned embodiments of the present application has a high success rate of needle insertion, reduced experimental cost, guaranteed experimental quality, and improved overall experimental results. Efficiency and other advantages.

Using the syringe needles disclosed in the above embodiments of the present application, according to the characteristics of the mouse tail veins being thin, brittle, and low pressure, a soft catheter and needle of a specific specification can be selected, and the needle can be inserted smoothly. , judging whether the needle is successfully inserted has high reliability; on this basis, continue to administer drugs or inject cells, avoiding the waste of injections caused by traditional operations, especially precious drugs, or the leakage of injections caused by trial needles. It will not affect the precise amount of medicine, reduce the bias of the experiment, and reduce the cost of the experiment.

After proper practice, experimenters can quickly master the injection skills of this method, ensuring a one-time injection success rate (greater than 90%). It is more intuitive and credible to judge whether the needle is inserted successfully based on the presence or absence of blood return than the "smooth and non-resistance feel" of the traditional method. The whole process only takes about 15 seconds, which is fast and accurate, and saves experimental time. In general, the present invention can greatly reduce the waste of experimental manpower, material resources, and financial resources, improve experimental efficiency and accuracy, and lay a solid foundation for further experimental operations.

Each embodiment in this specification is described in a progressive manner, each embodiment focuses on the difference from other embodiments, and the same and similar parts of each embodiment can be referred to each other. As for the device or method disclosed in the embodiment, since it corresponds to the method or device disclosed in the embodiment, the description is relatively simple, and for relevant details, please refer to the description of the method part.

The above description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the present invention will not be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (10)

1. a kind of syringe needle, including：Pintle and pin stalk are it is characterised in that described pin stalk includes：Before separate pin stalk Section and pin obstruct rear, and the first end that described pin obstructs leading portion is syringe needle, and the first end that described pin obstructs rear is connected with described pintle；

Described syringe needle also includes：Transparent flexible catheter, one end of described flexible catheter obstructs the second end of leading portion with described pin Docking, the second end that the other end of described flexible catheter obstructs rear with described pin is docked, and the internal diameter at described flexible catheter two ends is not more than The external diameter of described pin stalk.

2. syringe needle according to claim 1 is it is characterised in that the internal diameter of described flexible catheter is not more than 0.28mm.

3. syringe needle according to claim 1 is it is characterised in that the external diameter of described pin stalk is not more than 0.3mm.

4. syringe needle according to claim 1 is it is characterised in that one end of described flexible catheter is set in described pin stalk Second end of leading portion, the other end of described flexible catheter is set in the second end that described pin obstructs rear.

5. a kind of using method of syringe is it is characterised in that be applied in mouse tail vein injection experiment, described syringe is pacified Equipped with syringe needle disclosed in claim 1-5 any one, method includes：

Using described pin obstruct leading portion needle tip on described injection point inserting needle, wherein, described injection point is：Predeterminable range up and down Place is tauted, the supravenous injection point of mousetail；Described vein is：It is straightened, wiped to venectasia using alcohol swab Vein afterwards and on user oriented mousetail side；

Described syringe is operated to carry out dosed administration or cell infusion.

6. the using method of syringe according to claim 5 it is characterised in that obstruct the needle tip of leading portion using described pin Inserting needle on described injection point, including：

Described pin is kept to obstruct leading portion with the angle of described mousetail in the range of predetermined angle, using the pin of described pin stalk leading portion Most advanced and sophisticated inserting needle, parallel inserting needle after inserting needle setpoint distance on described injection point.

7. the using method of syringe according to claim 6 is it is characterised in that described predetermined angle scope is：Not little In 10 °, no more than 20 °.

8. the using method of syringe according to claim 6 it is characterised in that obstruct the needle tip of leading portion using described pin Inserting needle on described injection point, including：

Risen at 1/3 position using tooth straight forceps fixation described pin stalk leading portion second end, the needle tip of leading portion is obstructed in institute using described pin State inserting needle on injection point, parallel inserting needle after inserting needle setpoint distance.

9. the using method of syringe according to claim 5 is it is characterised in that described mice is postanesthetic Mus alive.

10. the using method of syringe according to claim 5 is it is characterised in that described predeterminable range is 1cm.