CN106365729A - Soil remediation organic fertilizer for overcoming panax notoginseng continuous cropping obstruction, preparation method and application of soil remediation organic fertilizer - Google Patents
Soil remediation organic fertilizer for overcoming panax notoginseng continuous cropping obstruction, preparation method and application of soil remediation organic fertilizer Download PDFInfo
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- CN106365729A CN106365729A CN201610737676.8A CN201610737676A CN106365729A CN 106365729 A CN106365729 A CN 106365729A CN 201610737676 A CN201610737676 A CN 201610737676A CN 106365729 A CN106365729 A CN 106365729A
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- 239000002689 soil Substances 0.000 title claims abstract description 62
- 238000009335 monocropping Methods 0.000 title claims abstract description 47
- 238000005067 remediation Methods 0.000 title claims abstract description 38
- 235000003143 Panax notoginseng Nutrition 0.000 title claims abstract description 31
- 241000180649 Panax notoginseng Species 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 239000003895 organic fertilizer Substances 0.000 title abstract 5
- 239000010902 straw Substances 0.000 claims abstract description 66
- 239000003337 fertilizer Substances 0.000 claims abstract description 48
- 238000000855 fermentation Methods 0.000 claims abstract description 40
- 230000004151 fermentation Effects 0.000 claims abstract description 40
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 14
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 14
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 11
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 11
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 11
- 239000002054 inoculum Substances 0.000 claims description 30
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 20
- 238000004519 manufacturing process Methods 0.000 claims description 20
- 239000012153 distilled water Substances 0.000 claims description 13
- 241000193755 Bacillus cereus Species 0.000 claims description 12
- 241000223259 Trichoderma Species 0.000 claims description 12
- 239000000084 colloidal system Substances 0.000 claims description 12
- 230000008485 antagonism Effects 0.000 claims description 10
- 239000004202 carbamide Substances 0.000 claims description 10
- 235000013877 carbamide Nutrition 0.000 claims description 10
- 238000011534 incubation Methods 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 235000013379 molasses Nutrition 0.000 claims description 10
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- 239000008103 glucose Substances 0.000 claims description 7
- 239000002361 compost Substances 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 238000003501 co-culture Methods 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- 239000013028 medium composition Substances 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000009331 sowing Methods 0.000 claims description 2
- 230000007480 spreading Effects 0.000 claims description 2
- 238000003892 spreading Methods 0.000 claims description 2
- 238000009333 weeding Methods 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 20
- 241000894006 Bacteria Species 0.000 abstract description 13
- 235000015097 nutrients Nutrition 0.000 abstract description 6
- 241000607479 Yersinia pestis Species 0.000 abstract description 5
- 230000002401 inhibitory effect Effects 0.000 abstract description 3
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 3
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 3
- 229910052700 potassium Inorganic materials 0.000 abstract description 3
- 241000499912 Trichoderma reesei Species 0.000 abstract description 2
- 238000009825 accumulation Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 239000000835 fiber Substances 0.000 abstract description 2
- 244000000034 soilborne pathogen Species 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 abstract 3
- 238000000354 decomposition reaction Methods 0.000 abstract 2
- 241000881860 Paenibacillus mucilaginosus Species 0.000 abstract 1
- 230000003042 antagnostic effect Effects 0.000 abstract 1
- 239000007633 bacillus mucilaginosus Substances 0.000 abstract 1
- 244000052616 bacterial pathogen Species 0.000 abstract 1
- 235000013601 eggs Nutrition 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 239000004016 soil organic matter Substances 0.000 abstract 1
- 230000001502 supplementing effect Effects 0.000 abstract 1
- 238000009423 ventilation Methods 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 description 6
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 239000000306 component Substances 0.000 description 4
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000009630 liquid culture Methods 0.000 description 4
- 239000012533 medium component Substances 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- 238000012549 training Methods 0.000 description 4
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- 235000009392 Vitis Nutrition 0.000 description 3
- 241000219095 Vitis Species 0.000 description 3
- 210000000481 breast Anatomy 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000013589 supplement Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 description 1
- 244000303040 Glycyrrhiza glabra Species 0.000 description 1
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 description 1
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 206010044278 Trace element deficiency Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000003967 crop rotation Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 229940010454 licorice Drugs 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
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- Genetics & Genomics (AREA)
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- Biomedical Technology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Soil Sciences (AREA)
- Mechanical Engineering (AREA)
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Abstract
The invention provides a soil remediation organic fertilizer for overcoming panax notoginseng continuous cropping obstruction, a preparation method and application of the soil remediation organic fertilizer. The soil remediation organic fertilizer is prepared by conducting mixed fermentation on crop straw, a straw decomposition agent and N, P and K fertilizer. The straw decomposition agent is obtained by conducting crossing antagonistic culture on four strains, wherein bacillus subtilis is used for inhibiting and killing harmful bacteria and pathogenic bacteria in soil, trichoderma reesei is used for decomposing straw fibers and quickly decomposing the straw, lactobacillus plantarum is used for inhibiting and killing pests and pest eggs in the soil, and bacillus mucilaginosus is used for removing autotoxicity residues of panax notoginseng. The microbial agent cooperates with the crop straw and the N, P and K fertilizer to achieve the effects of overcoming continuous cropping obstruction inducements such as soil-borne pathogen accumulation, soil nutrient unbalance and plant autotoxicity, supplementing soil organic matter and optimizing the aggregate structure of the soil, therefore, water retention, water drainage and ventilation are achieved, and crops are developed in root system and healthy and strong in plant.
Description
Technical field
The invention belongs to agricultural fertilizer field and in particular to a kind of soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle,
Preparation method and its usage.
Background technology
At present, the many regional scales having defined some high added value crops of China, industrialization, specialized kind
Plant, much area and peasant household all had oneself brand, public praise, customers.But due to continuous cropping continuous cropping obstacle, cause
The land resource scarcity in the place of production.Many peasant households have to crop rotation or Planting in the different location, have a strong impact on market efficiency and sustainable development.
When continuous cropping continuous cropping obstacle refers to continuously plant same crop or nearly edge crop in same soil, even if
Under the conditions of normal cultivation management, also occur plant strain growth die down, the phenomenon of yield reduction, quality decline.Main cause
It is: (1) soil transmissibility pest and disease damage increases: soil-borne disease insect pest is the topmost factor in the continuous cropping obstacle factor.After carrying out continuous cropping, due to
Its soil physico-chemical property and illumination, humiture, the change of gas, the life of some beneficial microbes (ammonium bacterium, nitrifier etc.)
Length is suppressed, and some harmful microorganisms are bred, and the balance of nature of soil microorganism is destroyed, and does not so only result in
The obstacle of manure digestion process, and pest and disease damage generation is many, it is fast to spread, and increase year by year, so that Producer can only lean on increasing
Dose and frequent medication, to control, cause the severe contamination to environment and agricultural product.(2) soil physical and chemical property changes: continuous cropping soil
Earth long-term cropping species is single, and crop has selectivity to the absorption of nutrition and fertilizer, therefore certainly will make after continuous cropping for many years
Become out of proportion, especially some trace element deficiencies of nutrient in soil.(3) Auto toxicity of plant: so-called Auto toxicity
Just refer to that some materials that certain plants discharges are of the same race to same stubble or lower stubble or growth of root of Roundfruit Licorice produces inhibitory action
Phenomenon.These materials can be secreted by overground part leaching, root system, plant residue the approach such as decomposes in soil and discharges,
They produce material impact to metabolic activities such as the photosynthesis of plant, Nutrient Absorption.
At present, the main method overcoming Radix Notoginseng continuous cropping continuous cropping obstacle is: efficent rotation, from disease-resistant variety, grafting and life
Thing is prevented and treated.Also there is no the fertilizer that can effectively overcome Radix Notoginseng continuous cropping continuous cropping obstacle, increase soil fertility in prior art.
Content of the invention
It is an object of the invention to provide a kind of soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle, overcome soil-borne disease former
Bacterium accumulation, soil nutrient is unbalance and plant self poisoning etc. continuous cropping continuous cropping obstacle inducement, supplement the soil organism, optimize soil
Crumb structure, enable water conservation, draining, ventilative, make crop root flourishing, plant is healthy and strong.
Present invention also offers a kind of preparation method of the soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle, through culture
Fermentation, prepares soil remediation fertilizer.
Present invention also offers a kind of purposes of the soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle, overcome Radix Notoginseng continuous cropping
Continuous cropping obstacle, improves yield.
A kind of preparation method of soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle that the present invention provides, walks including following
Rapid:
By crushed stalk, add straw decomposing inoculant, compost fermentation, prepared soil remediation fertilizer.
Further, crushed stalk is to 3～5cm；
Compost fermentation method particularly includes: after straw and straw decomposing inoculant mixing, build heap fermentation, fermentation temperature was at the 3rd day
After reaching 60-70 DEG C, once, water content is maintained at 50%～80%, ph value for 6.5～7.5 for daily turning；After fermentation three days,
Add n, p, k fertile, continue fermentation, the tenth day afterwards, temperature is 30-40 DEG C, water content is 30%～50%, turning one in every two days
Secondary；After fermenting the 15th day, temperature is identical with room temperature, and water content is 15%～25%, prepared soil remediation fertilizer.
Further, the addition of straw decomposing inoculant is that straw per ton adds 3～5kg straw decomposing inoculant.Fertile the adding of n, p, k
Entering amount is, and: n is fertile: straw per ton adds 1～5kg, and p is fertile: straw per ton adds 0.5～2kg, and k is fertile: straw per ton adds 0.5～
1kg.
Further, the preparation method of described straw decomposing inoculant is:
(1) bacillus subtilises, Li Trichoderma, Lactobacillus plantarum and colloid bacillus cereus are inoculated in solid training respectively
Foster base, culture；
(2) strain after step (1) culture is inoculated in fluid medium respectively, culture；
(3) strain after step (2) culture is respectively placed in fermentation tank, and volume production is cultivated；
(4) by the strain mixing after the culture of step (3) volume production, antagonism culture, prepared straw decomposing inoculant are intersected.
Bacillus subtilises (bacillus subtilis), described Li Trichoderma described in step (1)
(trichoderma reesei) described Lactobacillus plantarum (lactobacillus plantarum) described colloid bacillus cereus
(bacillusmucilaginosuskrassilnikov), all buy from market.
Cultivate described in step (1), condition is at 30 DEG C of temperature, cultivates 36～48h.
In step (1), the solid medium of four kinds of strains is agar.
Cultivate described in step (2), condition is: at 30 DEG C, shaking flask speed 100r/min, cultivate 36～48h.Step (2)
Middle liquid culture based component is: sucrose 10g, k2hpo40.2g、caco35g, nacl 0.4g, distilled water is settled to 1l, four kinds
The culture medium of bacterium is identical.
Volume production described in step (3) is cultivated particularly as follows: fermentation medium components are: glucose 30～60g/l, and molasses 50～
100g/l, carbamide 1～10g/l, kh2po45～10g/l, remaining is distilled water；Ph value 6.5～7.5；Volume production condition of culture is:
Strain inoculum concentration is 3～10%；Lower culture 120h at 30 DEG C, in incubation, every 12h stirs 5min, and every 2h inflates 10min.
After step (3) culture, in fermentation liquid, bacterium number reaches 10～15x1010/ml.
In step (4), strain presses the mixing of following part by weight: bacillus subtilises 10～50%, Li Trichoderma 10～
40%th, Lactobacillus plantarum 5～20%, colloid bacillus cereus 10～50%.
Antagonism culture is intersected, used medium composition is: glucose 20～40g/l described in step (4), molasses 30～
50g/l, carbamide 5～12g/l, p2o55～10g/l, kh2po42～10g/l, remaining is distilled water ph value 6.5～7.5.
Antagonism culture is intersected, condition is: in incubation, every 12h stirs 5min, every 2h inflation described in step (4)
10min, co-cultures 120h.
A kind of soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle that the present invention provides, is prepared into using above method
Arrive.
A kind of soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle that the present invention provides is used as to overcome Radix Notoginseng continuous cropping continuous cropping
Obstacle, improves its yield.
Specifically used method is: in site preparation, uniform spreading on the ground, 3～4 tons/mu.And/or, and compound fertilizer, specially
With fertile mixing, spread on the ground, ploughing and weeding is buried 35～45cm.Rest and reorganize 1 year.And/or, site preparation ditching, ridging, furrow width 60～
80cm, deep 80cm, row spacing 1.8～2m, sowing of transplanting seedlings, pour root water.
Compared with prior art, the present invention is by agricultural crop straw and straw decomposing inoculant and n, p, k fertilizer mixed fermentation, wherein,
Straw supplements the soil organism, builds soil water-retaining, draining, ventilative optimum crumb structure.Straw decomposing inoculant utilizes four kinds of bacterium
Plant mixed culture fermentation to prepare；In incubation, the composition of culture medium act as: glucose: provides strain to spread cultivation at the beginning of volume production
The nutrient of phase；Molasses: the nutrient that strain spreads cultivation volume production middle and late stage is provided；Carbamide: provide strain to spread cultivation the nitrogen needed for volume production；
Potassium dihydrogen phosphate: provide strain to spread cultivation phosphorus needed for volume production, potassium element.Through intersecting antagonism culture, its effect divides four kinds of strains
It is not: bacillus subtilises；Harmful bacteria in suppression and killing soil, pathogen.Li Trichoderma；Decomposing straw fiber, soon
Speed is become thoroughly decomposed straw.Lactobacillus plantarum: the insect in suppression and killing soil and worm's ovum.Colloid bacillus cereus: release Radix Notoginseng from
Poison residual.Being provided as soil in conjunction with n, p, k fertilizer provides fertility, nutrient.In a word, agricultural crop straw and straw decomposing inoculant in the present invention
Mutually work in coordination with n, p, k fertilizer and play a role, overcome the continuous cropping such as soil-borne pathogen is accumulated, soil nutrient is unbalance and plant self is poisoned
The inducement of continuous cropping obstacle, supplements the soil organism, optimizes the crumb structure of soil, enables water conservation, draining, breathes freely, makes work
Thing well developed root system, plant is healthy and strong, improves yield.
Specific embodiment
Embodiment 1
A kind of preparation method of the soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle, comprises the following steps:
By crops stalk crushing to 3～5cm, straw per ton adds 5kg straw decomposing inoculant, straw and straw decomposing inoculant to mix
After conjunction, build heap fermentation, after fermentation temperature reached 60-70 DEG C at the 3rd day, daily turning once, water content is maintained at 50%～
80%, ph value is 6.5～7.5；Being subsequently adding the fertile addition of n, p, k fertilizer n, p, k is: n is fertile: straw per ton adds 5kg, and p is fertile:
Straw per ton adds 1.5kg, and k is fertile: straw per ton adds 0.5kg；Continue fermentation, the tenth day afterwards, temperature is 30-40 DEG C, contains
The water yield is 30%～50%, and turning in every two days is once；After fermenting the 15th day, temperature is identical with room temperature, water content be 15%～
25%, prepared soil remediation fertilizer.
The preparation method of described straw decomposing inoculant is:
(1) bacillus subtilises, Li Trichoderma, Lactobacillus plantarum and colloid bacillus cereus are inoculated in solid training respectively
Foster base, condition is at 30 DEG C of temperature, cultivates 36～48h.；
(2) strain after step (1) culture is inoculated in fluid medium respectively, culture, at 30 DEG C, shaking flask speed
100r/min, cultivates 36～48h；Liquid culture based component is: sucrose 10g, k2hpo40.2g, caco35g, nacl 0.4g,
Distilled water is settled to 1l, and the culture medium of four kinds of bacterium is identical.
(3) strain after step (2) culture is respectively placed in fermentation tank, and volume production is cultivated；Fermentation medium components are: Fructus Vitis viniferae
Sugared 30～60g/l, molasses 50～100g/l, carbamide 1～10g/l, kh2po45～10g/l, remaining is distilled water；Ph value 6.5～
7.5；Volume production condition of culture is: strain inoculum concentration is 3～10%；Lower culture 120h at 30 DEG C, every 12h stirring in incubation
5min, every 2h inflates 10min.After culture, in fermentation liquid, bacterium number reaches 10～15x1010/ml
(4) strain after the culture of step (3) volume production is pressed bacillus subtilises 45%, Li Trichoderma 15%, plant breast
Bacillus 20%, the mixing of colloid bacillus cereus 20% ratio, intersect antagonism culture, used medium composition is: glucose 20～
40g/l, molasses 30～50g/l, carbamide 5～12g/l, p2o55～10g/l, kh2po42～10g/l, remaining is distilled water ph
Value 6.5～7.5.Condition is: in incubation, every 12h stirs 5min, and every 2h inflates 10min, co-cultures 120h, and prepared straw is rotten
Ripe dose.
In the trial zone of the soil remediation fertilizer of embodiment 1 preparation, 3 tons every mu of usage amount average out to.
Result of the test；First Year seed bar survival rate 96%, Second Year n plant survival rate is 90%.
Embodiment 2
A kind of preparation method of the soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle, comprises the following steps:
By crops stalk crushing to 3～5cm, straw per ton adds 5kg straw decomposing inoculant, straw and straw decomposing inoculant to mix
After conjunction, build heap fermentation, after fermentation temperature reached 60-70 DEG C at the 3rd day, daily turning once, water content is maintained at 50%～
80%, ph value is 6.5～7.5；It is subsequently adding n, p, k fertilizer, the fertile addition of n, p, k is:: n is fertile: straw per ton adds 3kg, p
Fertile: straw per ton adds 0.7kg, k is fertile: straw per ton adds 1kg；Continue fermentation, the tenth day afterwards, temperature is 30-40 DEG C, contains
The water yield is 30%～50%, and turning in every two days is once；After fermenting the 15th day, temperature is identical with room temperature, water content be 15%～
25%, prepared soil remediation fertilizer.
The preparation method of described straw decomposing inoculant is:
(1) bacillus subtilises, Li Trichoderma, Lactobacillus plantarum and colloid bacillus cereus are inoculated in solid training respectively
Foster base, condition is at 30 DEG C of temperature, cultivates 36～48h.；
(2) strain after step (1) culture is inoculated in fluid medium respectively, culture, at 30 DEG C, shaking flask speed
100r/min, cultivates 36～48h；Liquid culture based component is: sucrose 10g, k2hpo40.2g, caco35g, nacl 0.4g,
Distilled water is settled to 1l, and the culture medium of four kinds of bacterium is identical.
(3) strain after step (2) culture is respectively placed in fermentation tank, and volume production is cultivated；Fermentation medium components are: Fructus Vitis viniferae
Sugared 30～60g/l, molasses 50～100g/l, carbamide 1～10g/l, kh2po45～10g/l, remaining is distilled water；Ph value 6.5～
7.5；Volume production condition of culture is: strain inoculum concentration is 3～10%；Lower culture 120h at 30 DEG C, every 12h stirring in incubation
5min, every 2h inflates 10min.After culture, in fermentation liquid, bacterium number reaches 10～15x1010/ml
(4) strain after the culture of step (3) volume production is pressed bacillus subtilises 30%, Li Trichoderma 20%, plant breast
Bacillus 15%, the mixing of colloid bacillus cereus 35% ratio, intersect antagonism culture, used medium composition is: glucose 20～
40g/l, molasses 30～50g/l, carbamide 5～12g/l, p2o55～10g/l, kh2po42～10g/l, remaining is distilled water ph
Value 6.5～7.5.Condition is: in incubation, every 12h stirs 5min, and every 2h inflates 10min, co-cultures 120h, and prepared straw is rotten
Ripe dose.
In the trial zone of the soil remediation fertilizer of embodiment 2 preparation, 3 tons every mu of usage amount average out to.
Result of the test；First Year seed bar survival rate 95%, Second Year n plant survival rate is 89%.
Embodiment 3
A kind of preparation method of the soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle, comprises the following steps:
By crops stalk crushing to 3～5cm, straw per ton adds 5kg straw decomposing inoculant, straw and straw decomposing inoculant to mix
After conjunction, build heap fermentation, after fermentation temperature reached 60-70 DEG C at the 3rd day, daily turning once, water content is maintained at 50%～
80%, ph value is 6.5～7.5；It is subsequently adding n, p, k fertilizer, the fertile addition of n, p, k is that n is fertile: straw per ton adds 2kg, p fertilizer:
Straw per ton adds 1kg, and k is fertile: straw per ton adds 0.6kg；Continue fermentation, the tenth day afterwards, temperature is 30-40 DEG C, aqueous
Measure as 30%～50%, turning in every two days is once；After fermenting the 15th day, temperature is identical with room temperature, water content be 15%～
25%, prepared soil remediation fertilizer.
The preparation method of described straw decomposing inoculant is:
(1) bacillus subtilises, Li Trichoderma, Lactobacillus plantarum and colloid bacillus cereus are inoculated in solid training respectively
Foster base, condition is at 30 DEG C of temperature, cultivates 36～48h.；
(2) strain after step (1) culture is inoculated in fluid medium respectively, culture, at 30 DEG C, shaking flask speed
100r/min, cultivates 36～48h；Liquid culture based component is: sucrose 10g, k2hpo40.2g, caco35g, nacl 0.4g,
Distilled water is settled to 1l, and the culture medium of four kinds of bacterium is identical.
(3) strain after step (2) culture is respectively placed in fermentation tank, and volume production is cultivated；Fermentation medium components are: Fructus Vitis viniferae
Sugared 30～60g/l, molasses 50～100g/l, carbamide 1～10g/l, kh2po45～10g/l, remaining is distilled water；Ph value 6.5～
7.5；Volume production condition of culture is: strain inoculum concentration is 3～10%；Lower culture 120h at 30 DEG C, every 12h stirring in incubation
5min, every 2h inflates 10min.After culture, in fermentation liquid, bacterium number reaches 10～15x1010/ml
(4) strain after the culture of step (3) volume production is pressed bacillus subtilises 15%, Li Trichoderma 35%, plant breast
Bacillus 15%, the mixing of colloid bacillus cereus 35% ratio, intersect antagonism culture, used medium composition is: glucose 20～
40g/l, molasses 30～50g/l, carbamide 5～12g/l, p2o55～10g/l, kh2po42～10g/l, remaining is distilled water ph
Value 6.5～7.5.Condition is: in incubation, every 12h stirs 5min, and every 2h inflates 10min, co-cultures 120h, and prepared straw is rotten
Ripe dose.
In the trial zone of the soil remediation fertilizer of embodiment 3 preparation, 3 tons every mu of usage amount average out to.
Result of the test；First Year seed bar survival rate 97%, Second Year n plant survival rate is 92%.
Claims (10)
1. a kind of preparation method of the soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle is it is characterised in that described preparation method
Comprise the following steps: crushed stalk adds straw decomposing inoculant, compost fermentation, prepared soil remediation fertilizer；
The preparation method of described straw decomposing inoculant is:
(1) bacillus subtilises, Li Trichoderma, Lactobacillus plantarum and colloid bacillus cereus are inoculated in solid culture respectively
Base, culture；
(2) strain after step (1) culture is inoculated in fluid medium respectively, culture；
(3) strain after step (2) culture is respectively placed in fermentation tank, and volume production is cultivated；
(4) by the strain mixing after the culture of step (3) volume production, antagonism culture, prepared straw decomposing inoculant are intersected.
2. the preparation method of the soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle according to claim 1, its feature exists
In compost fermentation method particularly includes: after straw straw and straw decomposing inoculant mixing, build heap fermentation, fermentation temperature reached at the 3rd day
To after 60-70 DEG C, once, water content is maintained at 50%～80%, ph value for 6.5～7.5 for daily turning；After fermentation three days, plus
Enter n, p, k fertile, continue fermentation, the tenth day afterwards, temperature is 30-40 DEG C, water content is 30%～50%, turning one in every two days
Secondary；After fermenting the 15th day, temperature is identical with room temperature, and water content is 15%～25%, prepared soil remediation fertilizer.
3. the preparation method of the soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle according to claim 1 and 2, its feature
It is, in compost fermentation, the addition of straw decomposing inoculant is that straw per ton adds 3～5kg straw decomposing inoculant.
4. the preparation method of the soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle according to claim 1 and 2, its feature
It is, in compost fermentation, the fertile addition of n, p, k is: n is fertile: straw per ton adds 1～5kg, and p is fertile: straw per ton adds 0.5
～2kg, k are fertile: straw per ton adds 0.5～1kg.
5. the preparation method of the soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle according to claim 1 and 2, its feature
It is, in the preparation method step (4) of straw decomposing inoculant, strain presses the mixing of following part by weight: bacillus subtilises 10～
50%th, Li Trichoderma 10～40%, Lactobacillus plantarum 5～20%, colloid bacillus cereus 10～50%.
6. overcome the preparation method of the soil remediation fertilizer of Radix Notoginseng continuous cropping obstacle, its feature according to claim 1 or 5
It is, intersect antagonism culture described in the preparation method step (4) of straw decomposing inoculant, used medium composition is: glucose 20
～40g/l, molasses 30～50g/l, carbamide 5～12g/l, p2o55～10g/l, kh2po42～10g/l, remaining is distilled water
Ph value 6.5～7.5.
7. the preparation method of the soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle according to claim 1 or 6, its feature
It is, intersects antagonism culture described in step (4), condition is: in incubation, every 12h stirs 5min, every 2h inflates 30min,
Co-culture 120h.
8. a kind of soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle is it is characterised in that adopt any one of claim 1-7 institute
The method of stating prepares.
9. a kind of purposes of the soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle is it is characterised in that for overcoming Radix Notoginseng continuous cropping
Continuous cropping obstacle, improves its yield.
10. the soil remediation fertilizer overcoming Radix Notoginseng continuous cropping obstacle according to claim 9 purposes it is characterised in that
The specifically used method overcoming the soil remediation fertilizer of Radix Notoginseng continuous cropping obstacle is: in site preparation, uniform spreading on the ground, 3
～4 tons/mu；And/or, and compound fertilizer, special fertilizer mixes, and spreads on the ground, and ploughing and weeding is buried 35～45cm.Rest and reorganize 1 year；With/
Or, site preparation ditching, ridging, furrow width 60～80cm, deep 80cm, row spacing 1.8～2m, sowing of transplanting seedlings, pour root water.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106916771A (en) * | 2017-04-25 | 2017-07-04 | 安徽瑞驰兰德生物科技有限公司 | A kind of straw decomposing inoculant and using this kind of straw decomposing inoculant realize agricultural crop straw year after year continuous cropping full dose on the spot also field method |
| CN108034589A (en) * | 2018-01-11 | 2018-05-15 | 云南农业大学 | A kind of method for screening pseudo-ginseng rhizosphere biocontrol microorganisms microorganism |
| CN110564650A (en) * | 2019-09-29 | 2019-12-13 | 云南农业大学 | pseudomonas mendii and application thereof |
| CN112314101A (en) * | 2020-11-11 | 2021-02-05 | 木篱笆(南京)农业科技有限公司 | Method for repairing soil with different continuous cropping obstacles by using composts of different plant residues |
| CN112913369A (en) * | 2021-01-22 | 2021-06-08 | 东北农业大学 | Method for preventing and controlling alkalization of sunshine greenhouse planting soil salt |
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| CN104694410A (en) * | 2013-12-06 | 2015-06-10 | 大连三科生物工程有限公司 | Preparation method of straw decomposition agent |
| CN105272769A (en) * | 2015-11-30 | 2016-01-27 | 潍坊泽宇生物科技有限公司 | Composite microbial straw decomposing agent and preparation method thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104694410A (en) * | 2013-12-06 | 2015-06-10 | 大连三科生物工程有限公司 | Preparation method of straw decomposition agent |
| CN105272769A (en) * | 2015-11-30 | 2016-01-27 | 潍坊泽宇生物科技有限公司 | Composite microbial straw decomposing agent and preparation method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106916771A (en) * | 2017-04-25 | 2017-07-04 | 安徽瑞驰兰德生物科技有限公司 | A kind of straw decomposing inoculant and using this kind of straw decomposing inoculant realize agricultural crop straw year after year continuous cropping full dose on the spot also field method |
| CN108034589A (en) * | 2018-01-11 | 2018-05-15 | 云南农业大学 | A kind of method for screening pseudo-ginseng rhizosphere biocontrol microorganisms microorganism |
| CN110564650A (en) * | 2019-09-29 | 2019-12-13 | 云南农业大学 | pseudomonas mendii and application thereof |
| CN112314101A (en) * | 2020-11-11 | 2021-02-05 | 木篱笆(南京)农业科技有限公司 | Method for repairing soil with different continuous cropping obstacles by using composts of different plant residues |
| CN112314101B (en) * | 2020-11-11 | 2023-10-24 | 木篱笆(南京)农业科技有限公司 | Method for repairing different continuous cropping obstacle soil by using different plant residue compost |
| CN112913369A (en) * | 2021-01-22 | 2021-06-08 | 东北农业大学 | Method for preventing and controlling alkalization of sunshine greenhouse planting soil salt |
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